Spg033869 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg033869
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationscaffold13: 33791071 .. 33793542 (-)
RNA-Seq ExpressionSpg033869
SyntenySpg033869
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCTGGTTTAGGGCAAATATCTATACCGTCGGTTTTTTTGGACCCATAAAAGGGACGACCGTGATATACCTTCGCAGTTCGCGCCAATGAGCAAGCAAGGCAATCGATGACGCGGTGGCTCGGCGCTCCAGAGATCGCCGCTCGAACGGAGTCGGTGATGAAGACGTCTCTCCGCTCCGGCACCGGACGGTGAGCCTTGTACATTTGTCTCTTTGTTTTCCTAGCTTTAATAAATAACAACTCCGAATTTCATCGTTTCATTGTCCACTTCTGGTCTGAACCAACGAGTTGTTGATTCTACAAATGCAGAATTCACTTCCTTCTCGAAGCATGGAGGCCTATTTCGTCAGTTACTTCTCCAGTTGAAGGCACCATTTCTACTGTATCGCCCAATACCCTACAAAATGGCGATGACGATGGCAACTTCAAGCGGGAATTTATGAAGTTCAAGTCACCTCGGAGTAGCATGACCACCGTACTTCAGAGTGGTAACCGTGAAGCTCAAAGGACCTCAATCATTAAGCTCCGGCGTGTTGCCCAGAAGCTCTACAAATCGAAGCGCTTTGCTCGTGCGCTAGAGGTTGGTAACGAACAGTTAGGAAATCGAAATTTCTCACTTCATTTTTATGCCATTGGAAGAATAGAATCGAAATCACAGAATATTGCATCTAACTATATAGTTCATATATTTAGGTACTAATGTTGATGGAAACTCGAAATAATTTGCGTATATCAACGGCTGATCATGCTCTCAGATTGGAATTGACCATCAAATTGCAAGGTTTACTGAAAGCTGAAGAATACTTCAATCAATTGCCCAACATAGCCTCTCAAAAAGCTTCATCTCTCCCCCTTCTTCATGGTTATGTCAAAGCGAGGAACACTGAGAAAGCTGAAGCCTTCATGGTGAAACTGAGGGACTTGGGACTGGTCGTAGACCCACATCTTTACAATGAGATGATGAAACTGTATGTGGCCACATCTCAAACTGAGAAAGTTCCTCTTGTCATAAGGGACATGAAGCAAAATCAAATAATGAGAAATGTTCTCTCTTACAACCTTTGGATGAATGCTTGCAGTGAGATATATGGGGTTGGGTCAGTGGAGTTGGTGTACAAAGAAATGCTGACTGATAAGAACGTTCAAGTGGGATGGAGCACCCTATCTACCCTGGCCAAAGTTTATATACAGGCAGGCCTTGTTAACAAAGCGTTTGCAGCCTTAAAAGATGCAGAGAAGAAGCTATCCCCATGTAACCGGCTTGGATATTTCTTCTTAATCACATTGTATGCCTCTTTGAACAATAAGGAGGGAGTTCTTCGACTATGGAGAGGTAGTAAAGCTGTAAGTGGCAGACCTACTTGTGCCAATTACATGTGTATATTGCTGTGTTTGGTGAAGCTAGGAGACATCGACGAGGCCGAGAAAATATTTAGGGAATGGGAGTTCGATTGTCGTAACTACGACATTAGAGTCTCCAATGTTCTTCTGGGTGCATATGTGAGAAATGGATTGTTAGAGAAGGCCGAGTCGTTACATATGCGCATATTGGAGAGGGGTGGTAGTCCAAATTGTAAGACATGGGAGATTCTAATGGAGGGATGGGTGAGAAGCCAAAACGTGGCTAGAGATATTGATTTTCTGAAGGATTTTGCTTTGTTGAAATGTCATGGTTGAAGTCTTCACTTCCTTTTGTACTGCCTATTGCATATTATTTGGAGAATCATGGGATGCTGGGAAGTGGGAACCATAAAATTTGAGTCTTTAACCTTTTTGGTTTTGAGCTTGTACATTGAAACGGCTTCTGGCGGCCTTCAAATGTTGGACCATTTCCCTTGCCATGGTCATAGCCTCAAAATCTCAAATGCAGACAAACATCAGACGTTTTTGGACCATTTATCCATCTGTTGACTGAAGGATTTGATAAGACAATCTGTTGGATCCTTCGGAATTGGGGAGGAAACTATTTGAAGAGGAAACTGTTGAAGTTGACGTGTTCTATCTCAACATTATGTGAAGTTGGATGTAAGTTTCAGTAAGTGTACTGGTGAAGGATCTGATACTGAATGTTATGAAGATTTTCTGCTATACGATACCTGTGCTAATTGATATGATCCACTACATATGTTTCGATAGAGCGTCTACATGTGCATTAATCTGTTGGTAATGCCCATAGAAGCATAGTTTGGCATGAAAAGCCTGCCTCTCCATTAGGGCATTTACATTTCCCCAAATGCAGATGCAAGATCCCTCGGCAATGGGAATAGGATGAATTTCCTGAGAATAAATTTGCTGTGTTATCTAGGAATGGATGCATCTGCAACCAATTTCCAGTATCTTTTACAGTAGTCTTTTGCCTTTTCTGTTAAGGTCTCTACTCCACTCTTGAACAAAAATAGATGCATTTCAATTATTAGTTTGAAACCGACTTCGAGGATTGAATGAGAATCAAATTTCTATATTATGAATAAT

mRNA sequence

ATGACGCGGTGGCTCGGCGCTCCAGAGATCGCCGCTCGAACGGAGTCGGTGATGAAGACGTCTCTCCGCTCCGGCACCGGACGAATTCACTTCCTTCTCGAAGCATGGAGGCCTATTTCGTCAGTTACTTCTCCAGTTGAAGGCACCATTTCTACTGTATCGCCCAATACCCTACAAAATGGCGATGACGATGGCAACTTCAAGCGGGAATTTATGAAGTTCAAGTCACCTCGGAGTAGCATGACCACCGTACTTCAGAGTGGTAACCGTGAAGCTCAAAGGACCTCAATCATTAAGCTCCGGCGTGTTGCCCAGAAGCTCTACAAATCGAAGCGCTTTGCTCGTGCGCTAGAGGTACTAATGTTGATGGAAACTCGAAATAATTTGCGTATATCAACGGCTGATCATGCTCTCAGATTGGAATTGACCATCAAATTGCAAGGTTTACTGAAAGCTGAAGAATACTTCAATCAATTGCCCAACATAGCCTCTCAAAAAGCTTCATCTCTCCCCCTTCTTCATGGTTATGTCAAAGCGAGGAACACTGAGAAAGCTGAAGCCTTCATGGTGAAACTGAGGGACTTGGGACTGGTCGTAGACCCACATCTTTACAATGAGATGATGAAACTGTATGTGGCCACATCTCAAACTGAGAAAGTTCCTCTTGTCATAAGGGACATGAAGCAAAATCAAATAATGAGAAATGTTCTCTCTTACAACCTTTGGATGAATGCTTGCAGTGAGATATATGGGGTTGGGTCAGTGGAGTTGGTGTACAAAGAAATGCTGACTGATAAGAACGTTCAAGTGGGATGGAGCACCCTATCTACCCTGGCCAAAGTTTATATACAGGCAGGCCTTGTTAACAAAGCGTTTGCAGCCTTAAAAGATGCAGAGAAGAAGCTATCCCCATGTAACCGGCTTGGATATTTCTTCTTAATCACATTGTATGCCTCTTTGAACAATAAGGAGGGAGTTCTTCGACTATGGAGAGGTAGTAAAGCTGTAAGTGGCAGACCTACTTGTGCCAATTACATGTGTATATTGCTGTGTTTGGTGAAGCTAGGAGACATCGACGAGGCCGAGAAAATATTTAGGGAATGGGAGTTCGATTGTCGTAACTACGACATTAGAGTCTCCAATGTTCTTCTGGGTGCATATGTGAGAAATGGATTGTTAGAGAAGGCCGAGTCGTTACATATGCGCATATTGGAGAGGGGTGGTAGTCCAAATTGTAAGACATGGGAGATTCTAATGGAGGGATGGGTGAGAAGCCAAAACGTGGCTAGAGATATTGATTTTCTGAAGGATTTTGCTTTGTTGAAATGTCATGGTTGA

Coding sequence (CDS)

ATGACGCGGTGGCTCGGCGCTCCAGAGATCGCCGCTCGAACGGAGTCGGTGATGAAGACGTCTCTCCGCTCCGGCACCGGACGAATTCACTTCCTTCTCGAAGCATGGAGGCCTATTTCGTCAGTTACTTCTCCAGTTGAAGGCACCATTTCTACTGTATCGCCCAATACCCTACAAAATGGCGATGACGATGGCAACTTCAAGCGGGAATTTATGAAGTTCAAGTCACCTCGGAGTAGCATGACCACCGTACTTCAGAGTGGTAACCGTGAAGCTCAAAGGACCTCAATCATTAAGCTCCGGCGTGTTGCCCAGAAGCTCTACAAATCGAAGCGCTTTGCTCGTGCGCTAGAGGTACTAATGTTGATGGAAACTCGAAATAATTTGCGTATATCAACGGCTGATCATGCTCTCAGATTGGAATTGACCATCAAATTGCAAGGTTTACTGAAAGCTGAAGAATACTTCAATCAATTGCCCAACATAGCCTCTCAAAAAGCTTCATCTCTCCCCCTTCTTCATGGTTATGTCAAAGCGAGGAACACTGAGAAAGCTGAAGCCTTCATGGTGAAACTGAGGGACTTGGGACTGGTCGTAGACCCACATCTTTACAATGAGATGATGAAACTGTATGTGGCCACATCTCAAACTGAGAAAGTTCCTCTTGTCATAAGGGACATGAAGCAAAATCAAATAATGAGAAATGTTCTCTCTTACAACCTTTGGATGAATGCTTGCAGTGAGATATATGGGGTTGGGTCAGTGGAGTTGGTGTACAAAGAAATGCTGACTGATAAGAACGTTCAAGTGGGATGGAGCACCCTATCTACCCTGGCCAAAGTTTATATACAGGCAGGCCTTGTTAACAAAGCGTTTGCAGCCTTAAAAGATGCAGAGAAGAAGCTATCCCCATGTAACCGGCTTGGATATTTCTTCTTAATCACATTGTATGCCTCTTTGAACAATAAGGAGGGAGTTCTTCGACTATGGAGAGGTAGTAAAGCTGTAAGTGGCAGACCTACTTGTGCCAATTACATGTGTATATTGCTGTGTTTGGTGAAGCTAGGAGACATCGACGAGGCCGAGAAAATATTTAGGGAATGGGAGTTCGATTGTCGTAACTACGACATTAGAGTCTCCAATGTTCTTCTGGGTGCATATGTGAGAAATGGATTGTTAGAGAAGGCCGAGTCGTTACATATGCGCATATTGGAGAGGGGTGGTAGTCCAAATTGTAAGACATGGGAGATTCTAATGGAGGGATGGGTGAGAAGCCAAAACGTGGCTAGAGATATTGATTTTCTGAAGGATTTTGCTTTGTTGAAATGTCATGGTTGA

Protein sequence

MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVEGTISTVSPNTLQNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALEVLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVKARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDIDEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEILMEGWVRSQNVARDIDFLKDFALLKCHG
Homology
BLAST of Spg033869 vs. NCBI nr
Match: XP_022997144.1 (pentatricopeptide repeat-containing protein At5g27460 isoform X1 [Cucurbita maxima])

HSP 1 Score: 729.9 bits (1883), Expect = 1.3e-206
Identity = 371/447 (83.00%), Postives = 406/447 (90.83%), Query Frame = 0

Query: 1   MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVEGT--ISTVSPNTL 60
           MTRWLGA E+AART+SVMKT+LRSGTGRIHFLL AWRPISS TSPV+ +  IS VSP TL
Sbjct: 1   MTRWLGASEVAARTKSVMKTTLRSGTGRIHFLLRAWRPISSHTSPVDSSDIISAVSPKTL 60

Query: 61  QNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALE 120
           Q G D+GNFK++FMK K PRSS+TTVLQSGN EAQRTSI KLRRV +KL KSKRF RALE
Sbjct: 61  QKGGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKSKRFERALE 120

Query: 121 VLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVK 180
           VLMLMET+N +R+S ADHALRLELTIK+ GLLKAEE+FNQLPNIASQKASSL LLHGYVK
Sbjct: 121 VLMLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVK 180

Query: 181 ARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLS 240
            +NTEKAEAFM KL+ LGLVVDPHLYNEMMKLYVATSQ EKVPLVI+DMKQNQI+RNVLS
Sbjct: 181 EQNTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNQILRNVLS 240

Query: 241 YNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDA 300
           YNLWMNACSE+YGVGSVELVY+EML+DKNVQVGWSTLSTLAKVY QAGLV+KAFAAL++A
Sbjct: 241 YNLWMNACSELYGVGSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALREA 300

Query: 301 EKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDI 360
           EKKLS  NRLGYFFLITLYA+LN+KEGVLR+W  SKAVS RPTCANYMCILLCLVKLGDI
Sbjct: 301 EKKLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDI 360

Query: 361 DEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEIL 420
           +EAEKIF+EWE DCRNYDIRVSNVLLGAYVRNGLL++AESLHM  LERGG+PN KTWEIL
Sbjct: 361 NEAEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLDQAESLHMHTLERGGTPNYKTWEIL 420

Query: 421 MEGWVRSQNVARDIDFL-KDFALLKCH 445
           MEG VRSQN+ R I+FL  DFA LKCH
Sbjct: 421 MEGCVRSQNLDRGINFLNNDFAGLKCH 447

BLAST of Spg033869 vs. NCBI nr
Match: XP_023545813.1 (pentatricopeptide repeat-containing protein At5g27460 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 728.8 bits (1880), Expect = 2.8e-206
Identity = 375/447 (83.89%), Postives = 402/447 (89.93%), Query Frame = 0

Query: 1   MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVEGT--ISTVSPNTL 60
           MTRWLGA E+AART SVMKT+LRSGTGRIHFLL AWRPISS TSPV+ +  IS VSP TL
Sbjct: 1   MTRWLGASEVAARTNSVMKTTLRSGTGRIHFLLRAWRPISSHTSPVDSSDIISAVSPKTL 60

Query: 61  QNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALE 120
           Q G D+GNFK++FMK KSPRSS+TTVLQSGN EAQRTSI KLRRV +KL KSKRF RALE
Sbjct: 61  QKGGDNGNFKQDFMKLKSPRSSVTTVLQSGNCEAQRTSITKLRRVVKKLCKSKRFERALE 120

Query: 121 VLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVK 180
           VLMLMET+N +R+S ADHALRLELTIKL GLLKAEE FNQLPNIASQKASSL LLHGYVK
Sbjct: 121 VLMLMETQNKIRMSPADHALRLELTIKLHGLLKAEELFNQLPNIASQKASSLLLLHGYVK 180

Query: 181 ARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLS 240
            +NTEKAEAFM KL+ LGLVVDPHLYNEMMKLYVATSQ EKVPLVI+DMKQN I+RNVLS
Sbjct: 181 EKNTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNHILRNVLS 240

Query: 241 YNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDA 300
           YNLWMNACSE+YGV SVELVY+EML+DKNVQVGWSTLSTLAKVY QAGLV+KAFAALK A
Sbjct: 241 YNLWMNACSELYGVRSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALKAA 300

Query: 301 EKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDI 360
           EKKLS  NRLGYFFLITLYA+LN+KEGVLR+W  SKAVS RPTCANYMCILLCLVKLGDI
Sbjct: 301 EKKLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDI 360

Query: 361 DEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEIL 420
            EAEKIF+EWE DCRNYDIRVSNVLLGAYVRNGLLEKAESLHM  LERGG+PN KTWEIL
Sbjct: 361 HEAEKIFQEWELDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMHTLERGGTPNYKTWEIL 420

Query: 421 MEGWVRSQNVARDIDFLK-DFALLKCH 445
           MEG VRSQN+ R I+FLK DFA LKCH
Sbjct: 421 MEGCVRSQNLDRGINFLKNDFAGLKCH 447

BLAST of Spg033869 vs. NCBI nr
Match: KAG6598713.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 719.9 bits (1857), Expect = 1.3e-203
Identity = 368/447 (82.33%), Postives = 401/447 (89.71%), Query Frame = 0

Query: 1   MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVEGT--ISTVSPNTL 60
           MT WLGA E+AART+SVMKT+LRSG GRIHFLL AWRPISS TSPV+ +  IS VSP TL
Sbjct: 1   MTLWLGASEVAARTKSVMKTTLRSGAGRIHFLLRAWRPISSHTSPVDSSDIISAVSPKTL 60

Query: 61  QNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALE 120
           Q G D+GNFK++FMK K PRSS+TTVLQSGN EAQRTSI KLRRV +KL KSKRF RALE
Sbjct: 61  QKGGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKSKRFERALE 120

Query: 121 VLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVK 180
            LMLMET+N +R+S ADHALRLELTIK+ GLLKAEE+FNQLPNIASQKASSL LLHGYVK
Sbjct: 121 ALMLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVK 180

Query: 181 ARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLS 240
            +NTEKAEAFM KL+ LGLVVDPHLYNEMMKLYVATSQ EKVPLVI+DMKQN I+RNVLS
Sbjct: 181 EKNTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNHILRNVLS 240

Query: 241 YNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDA 300
           YNLWMNACSE+YGV SVELVY+EML+DKNVQVGWSTLSTLAKVY QAGLV+KAFAALK A
Sbjct: 241 YNLWMNACSELYGVRSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALKAA 300

Query: 301 EKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDI 360
           EKKLS  NRLGYFFLITLYA+LN+KEGVLR+W  SKAVS RPTCANYMCILLCLVKLGDI
Sbjct: 301 EKKLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDI 360

Query: 361 DEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEIL 420
           +EAEKIF+EWE DCRNYDIRVSNVLLGAYVRNGLLEKAESLHM  LERGG+PN KTWEIL
Sbjct: 361 NEAEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMHTLERGGTPNYKTWEIL 420

Query: 421 MEGWVRSQNVARDIDFLK-DFALLKCH 445
           MEG VRSQN+ R I+FL+ +FA LKCH
Sbjct: 421 MEGCVRSQNLDRGINFLENNFAGLKCH 447

BLAST of Spg033869 vs. NCBI nr
Match: XP_038885083.1 (pentatricopeptide repeat-containing protein At5g27460 [Benincasa hispida])

HSP 1 Score: 719.5 bits (1856), Expect = 1.7e-203
Identity = 367/443 (82.84%), Postives = 395/443 (89.16%), Query Frame = 0

Query: 1   MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVE--GTISTVSPNTL 60
           MTRWL A E+AARTESVMK  LRSGT RIHFL +AW+PISS+TSPV+    IS VSP TL
Sbjct: 14  MTRWLRASEVAARTESVMKAPLRSGTERIHFLFQAWKPISSLTSPVDSLSNISAVSPKTL 73

Query: 61  QNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALE 120
           QN  DDGN K+EFMK K PRSS++TVLQSG  EAQRTSI KLRRV +KLY SKRF RALE
Sbjct: 74  QNSVDDGNSKQEFMKLKLPRSSVSTVLQSGKCEAQRTSITKLRRVVKKLYTSKRFERALE 133

Query: 121 VLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVK 180
           VLMLMETR+NLR+S A+HALRLELTIK+ G+LKAEEYFNQLP IASQKASSLPLLHGYVK
Sbjct: 134 VLMLMETRDNLRMSPAEHALRLELTIKVHGVLKAEEYFNQLPTIASQKASSLPLLHGYVK 193

Query: 181 ARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLS 240
            RNTEKAEAFMVKLRDLGLVV+ HLYNEMMKLYVATSQ EKVPLVIRDMKQNQI RNVLS
Sbjct: 194 GRNTEKAEAFMVKLRDLGLVVNHHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIQRNVLS 253

Query: 241 YNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDA 300
           YNLWMNACSE+YGVGSVELV++EMLTDKNVQVGWSTLSTLAKVYIQ  LV+KAFAALKDA
Sbjct: 254 YNLWMNACSEVYGVGSVELVFEEMLTDKNVQVGWSTLSTLAKVYIQEDLVDKAFAALKDA 313

Query: 301 EKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDI 360
           EKKLSPCNRLGYFFLITLYASL +K+GV R+WR SKAVSGRPTCANYMCILLCLVK+GDI
Sbjct: 314 EKKLSPCNRLGYFFLITLYASLKDKKGVSRVWRASKAVSGRPTCANYMCILLCLVKVGDI 373

Query: 361 DEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEIL 420
           DEAEK+F+EWE +CRNYDIRVSNVLLGAYVRNGLLEKAESLHM  L RGGSPN KTWEIL
Sbjct: 374 DEAEKVFKEWELNCRNYDIRVSNVLLGAYVRNGLLEKAESLHMHTLGRGGSPNYKTWEIL 433

Query: 421 MEGWVRS-QNVARDIDFLKDFAL 441
           MEGW R+ QNV R I FL+   L
Sbjct: 434 MEGWARNQQNVDRAISFLRKILL 456

BLAST of Spg033869 vs. NCBI nr
Match: XP_022961834.1 (pentatricopeptide repeat-containing protein At5g27460 isoform X1 [Cucurbita moschata])

HSP 1 Score: 718.8 bits (1854), Expect = 2.9e-203
Identity = 367/447 (82.10%), Postives = 402/447 (89.93%), Query Frame = 0

Query: 1   MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVEGT--ISTVSPNTL 60
           MTRWLGA E+AART+SVMKT+LRSGTGRI+FLL AWRPISS TSPV+ +  IS VSP TL
Sbjct: 1   MTRWLGASEVAARTKSVMKTTLRSGTGRINFLLRAWRPISSHTSPVDSSDIISAVSPKTL 60

Query: 61  QNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALE 120
           Q G D+GNFK++FMK K PRSS+TTVLQSGN EAQRTSI KLRRV +KL K KRF RALE
Sbjct: 61  QKGGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKPKRFERALE 120

Query: 121 VLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVK 180
           VLMLMET+N +R+S ADHALRLELTIK+ GLLKAEE+FNQLPNIASQKASSL LLHGYVK
Sbjct: 121 VLMLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVK 180

Query: 181 ARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLS 240
            +NTEKAEAFM KL+ LGLVVDPHLYNEMMKLYVATSQ EKVPLVI+DMKQN I+RNVLS
Sbjct: 181 EKNTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNHILRNVLS 240

Query: 241 YNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDA 300
           YNLWMNACSE+YG  SVELVY+EML+DKNVQVGWSTLSTLAKVY QAGLV+KAFAALK A
Sbjct: 241 YNLWMNACSELYGFRSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALKAA 300

Query: 301 EKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDI 360
           EKKLS  NRLGYFFLITLYA+LN+KEGVLR+W  SKAVS RPTCANYMCILLCLVKLGD+
Sbjct: 301 EKKLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDL 360

Query: 361 DEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEIL 420
           +EAEKIF+EWE DCRNYDIRVSNVLLGAYVRNGLLEKAESLHM  LERGG+PN KTWEIL
Sbjct: 361 NEAEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMHTLERGGTPNYKTWEIL 420

Query: 421 MEGWVRSQNVARDIDFLK-DFALLKCH 445
           MEG VRSQN+ R I+FL+ +FA LKCH
Sbjct: 421 MEGCVRSQNLDRGINFLENNFAGLKCH 447

BLAST of Spg033869 vs. ExPASy Swiss-Prot
Match: Q3E911 (Pentatricopeptide repeat-containing protein At5g27460 OS=Arabidopsis thaliana OX=3702 GN=At5g27460 PE=2 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 2.2e-113
Identity = 212/410 (51.71%), Postives = 286/410 (69.76%), Query Frame = 0

Query: 41  SVTSPVEGTISTVSPNTLQNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKL 100
           + T  V   +S+++  +  +   + N  +E ++   PR S+T++LQ         S+ +L
Sbjct: 14  NTTGVVSRLVSSLADGSDTSSVANRNSLKEILRKNGPRRSVTSLLQERIDSGHAVSLSEL 73

Query: 101 RRVAQKLYKSKRFARALEVLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQL- 160
           R ++++L +S R+  AL+++  ME + ++  S  D ALRL+L IK  GL + EEYF +L 
Sbjct: 74  RLISKRLIRSNRYDLALQMMEWMENQKDIEFSVYDIALRLDLIIKTHGLKQGEEYFEKLL 133

Query: 161 ---PNIASQKASSLPLLHGYVKARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQ 220
               ++   K++ LPLL  YVK +  ++AEA M KL  LG +V PH +NEMMKLY A+ Q
Sbjct: 134 HSSVSMRVAKSAYLPLLRAYVKNKMVKEAEALMEKLNGLGFLVTPHPFNEMMKLYEASGQ 193

Query: 221 TEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLS 280
            EKV +V+  MK N+I RNVLSYNLWMNAC E+ GV +VE VYKEM+ DK+V+VGWS+L 
Sbjct: 194 YEKVVMVVSMMKGNKIPRNVLSYNLWMNACCEVSGVAAVETVYKEMVGDKSVEVGWSSLC 253

Query: 281 TLAKVYIQAGLVNKAFAALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAV 340
           TLA VYI++G   KA   L+DAEK L+  NRLGYFFLITLYASL NKEGV+RLW  SK+V
Sbjct: 254 TLANVYIKSGFDEKARLVLEDAEKMLNRSNRLGYFFLITLYASLGNKEGVVRLWEVSKSV 313

Query: 341 SGRPTCANYMCILLCLVKLGDIDEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKA 400
            GR +C NY+C+L  LVK GD++EAE++F EWE  C NYD+RVSNVLLGAYVRNG + KA
Sbjct: 314 CGRISCVNYICVLSSLVKTGDLEEAERVFSEWEAQCFNYDVRVSNVLLGAYVRNGEIRKA 373

Query: 401 ESLHMRILERGGSPNCKTWEILMEGWVRSQNVARDIDFLKDFALL--KCH 445
           ESLH  +LERGG+PN KTWEILMEGWV+ +N+ + ID +    +L  +CH
Sbjct: 374 ESLHGCVLERGGTPNYKTWEILMEGWVKCENMEKAIDAMHQVFVLMRRCH 423

BLAST of Spg033869 vs. ExPASy Swiss-Prot
Match: Q8LPS6 (Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX=3702 GN=At1g02150 PE=2 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 2.8e-63
Identity = 132/364 (36.26%), Postives = 216/364 (59.34%), Query Frame = 0

Query: 75  KSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALEVLMLMETR-NNLRIST 134
           + P     +VL    +  ++ +  +L RV ++L K KR  +ALEV   M  R    R+S 
Sbjct: 77  EKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKRANQALEVYDWMNNRGERFRLSA 136

Query: 135 ADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVKARNTEKAEAFMVKLR 194
           +D A++L+L  K++G+  AEE+F QLP     +     LL+ YV+A++ EKAEA +  +R
Sbjct: 137 SDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKSREKAEALLNTMR 196

Query: 195 DLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEIYGVG 254
           D G  + P  +N MM LY+   + +KV  ++ +MKQ  I  ++ SYN+W+++C  +  V 
Sbjct: 197 DKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNIWLSSCGSLGSVE 256

Query: 255 SVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDAEKKLSPCNRLGYFFL 314
            +ELVY++M +D ++   W+T ST+A +YI+ G   KA  AL+  E +++  NR+ Y +L
Sbjct: 257 KMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEARITGRNRIPYHYL 316

Query: 315 ITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDIDEAEKIFREWEFDCR 374
           ++LY SL NK+ + R+W   K+V        Y  ++  LV++GDI+ AEK++ EW     
Sbjct: 317 LSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDIEGAEKVYEEWLPVKS 376

Query: 375 NYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEILMEGWVRSQNVARDID 434
           +YD R+ N+L+ AYV+N  LE AE L   ++E GG P+  TWEIL  G  R + ++  + 
Sbjct: 377 SYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEILAVGHTRKRCISEALT 436

Query: 435 FLKD 438
            L++
Sbjct: 437 CLRN 440

BLAST of Spg033869 vs. ExPASy Swiss-Prot
Match: O22714 (Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX=3702 GN=At1g60770 PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.1e-58
Identity = 121/329 (36.78%), Postives = 188/329 (57.14%), Query Frame = 0

Query: 105 QKLYKSKRFARALEVLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIAS 164
           +KL     +  AL++  +ME R  +  + +D A+ L+L  K + +   E YF  LP  + 
Sbjct: 63  KKLRNRGLYYPALKLSEVMEER-GMNKTVSDQAIHLDLVAKAREITAGENYFVDLPETSK 122

Query: 165 QKASSLPLLHGYVKARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVI 224
            + +   LL+ Y K   TEKAE  + K+++L +      YN +M LY  T +TEKVP +I
Sbjct: 123 TELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVPAMI 182

Query: 225 RDMKQNQIMRNVLSYNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQ 284
           +++K   +M +  +YN+WM A +    +  VE V +EM  D  V   W+T S +A +Y+ 
Sbjct: 183 QELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASIYVD 242

Query: 285 AGLVNKAFAALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCAN 344
           AGL  KA  AL++ E K +  +   Y FLITLY  L     V R+WR  +    + +   
Sbjct: 243 AGLSQKAEKALQELEMKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTSNVA 302

Query: 345 YMCILLCLVKLGDIDEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRIL 404
           Y+ ++  LVKL D+  AE +F+EW+ +C  YDIR+ NVL+GAY + GL++KA  L  +  
Sbjct: 303 YLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKEKAP 362

Query: 405 ERGGSPNCKTWEILMEGWVRSQNVARDID 434
            RGG  N KTWEI M+ +V+S ++AR ++
Sbjct: 363 RRGGKLNAKTWEIFMDYYVKSGDMARALE 390

BLAST of Spg033869 vs. ExPASy Swiss-Prot
Match: Q9SY07 (Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g02820 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.2e-51
Identity = 124/429 (28.90%), Postives = 225/429 (52.45%), Query Frame = 0

Query: 15  ESVMKTSLRSGTGRIH--FLLEAWRPISSVTSPV--------EGTISTVSPNTLQNGDDD 74
           ++++  S R     IH  F   A   + + T+PV        +G  S     T+  G D 
Sbjct: 3   KNMLVRSARPTLASIHRLFSAAAAATVDTATAPVVKPRSGGGKGGESANKKETVVGGRD- 62

Query: 75  GNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALEVLMLME 134
                  +     + S    ++    E       +L R+ ++L K KR+  ALE+   M 
Sbjct: 63  -TLGGRLLSLVYTKRSAVVTIRKWKEEGHSVRKYELNRIVRELRKIKRYKHALEICEWMV 122

Query: 135 TRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVKARNTEK 194
            + ++++   D+A+ L+L  K++GL  AE++F  +P+     A+   LLH YV+ + ++K
Sbjct: 123 VQEDIKLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTSLLHSYVQNKLSDK 182

Query: 195 AEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLSYNLWMN 254
           AEA   K+ + G +     YN M+ +Y++  Q EKVP++I+++K  +   ++++YNLW+ 
Sbjct: 183 AEALFEKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKELK-IRTSPDIVTYNLWLT 242

Query: 255 ACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDAEKKLSP 314
           A +    V   E VY +   +K +   W T S L  +Y +   V KA  ALK+ EK +S 
Sbjct: 243 AFASGNDVEGAEKVYLKAKEEK-LNPDWVTYSVLTNLYAKTDNVEKARLALKEMEKLVSK 302

Query: 315 CNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDIDEAEKI 374
            NR+ Y  LI+L+A+L +K+GV   W+  K+   +   A Y+ ++  +VKLG+ ++A+ +
Sbjct: 303 KNRVAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMISAVVKLGEFEQAKGL 362

Query: 375 FREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEILMEGWVR 434
           + EWE      D R+ N++L  Y+    +   E  + RI+E+G +P+  TWEIL   +++
Sbjct: 363 YDEWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGINPSYSTWEILTWAYLK 422

BLAST of Spg033869 vs. ExPASy Swiss-Prot
Match: Q9FZ24 (Pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g02370 PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.3e-49
Identity = 111/321 (34.58%), Postives = 180/321 (56.07%), Query Frame = 0

Query: 100 LRRVAQKLYKSKRFARALEVLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQL 159
           L R A+ L K +R   A E+   ME R  +  S +DHA+ L+L  K +GL  AE YFN L
Sbjct: 106 LFRCAKTLRKFRRPQHAFEIFDWMEKR-KMTFSVSDHAICLDLIGKTKGLEAAENYFNNL 165

Query: 160 -PNIASQKASSLPLLHGYVKARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTE 219
            P+  + +++   L++ Y      EKA+A    + +L  V +   +N MM +Y+  SQ E
Sbjct: 166 DPSAKNHQSTYGALMNCYCVELEEEKAKAHFEIMDELNFVNNSLPFNNMMSMYMRLSQPE 225

Query: 220 KVPLVIRDMKQNQIMRNVLSYNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTL 279
           KVP+++  MKQ  I    ++Y++WM +C  +  +  +E +  EM  D   +  W+T S L
Sbjct: 226 KVPVLVDAMKQRGISPCGVTYSIWMQSCGSLNDLDGLEKIIDEMGKDSEAKTTWNTFSNL 285

Query: 280 AKVYIQAGLVNKAFAALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSG 339
           A +Y +AGL  KA +ALK  E+K++P NR  + FL++LYA ++    V R+W   K    
Sbjct: 286 AAIYTKAGLYEKADSALKSMEEKMNPNNRDSHHFLMSLYAGISKGPEVYRVWESLKKARP 345

Query: 340 RPTCANYMCILLCLVKLGDIDEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAES 399
                +Y+ +L  + KLGD+D  +KIF EWE  C  YD+R++N+ +  Y++  + E+AE 
Sbjct: 346 EVNNLSYLVMLQAMSKLGDLDGIKKIFTEWESKCWAYDMRLANIAINTYLKGNMYEEAEK 405

Query: 400 LHMRILERGGSPNCKTWEILM 420
           +    +++   P  K  ++LM
Sbjct: 406 ILDGAMKKSKGPFSKARQLLM 425

BLAST of Spg033869 vs. ExPASy TrEMBL
Match: A0A6J1KAM1 (pentatricopeptide repeat-containing protein At5g27460 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492138 PE=4 SV=1)

HSP 1 Score: 729.9 bits (1883), Expect = 6.2e-207
Identity = 371/447 (83.00%), Postives = 406/447 (90.83%), Query Frame = 0

Query: 1   MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVEGT--ISTVSPNTL 60
           MTRWLGA E+AART+SVMKT+LRSGTGRIHFLL AWRPISS TSPV+ +  IS VSP TL
Sbjct: 1   MTRWLGASEVAARTKSVMKTTLRSGTGRIHFLLRAWRPISSHTSPVDSSDIISAVSPKTL 60

Query: 61  QNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALE 120
           Q G D+GNFK++FMK K PRSS+TTVLQSGN EAQRTSI KLRRV +KL KSKRF RALE
Sbjct: 61  QKGGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKSKRFERALE 120

Query: 121 VLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVK 180
           VLMLMET+N +R+S ADHALRLELTIK+ GLLKAEE+FNQLPNIASQKASSL LLHGYVK
Sbjct: 121 VLMLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVK 180

Query: 181 ARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLS 240
            +NTEKAEAFM KL+ LGLVVDPHLYNEMMKLYVATSQ EKVPLVI+DMKQNQI+RNVLS
Sbjct: 181 EQNTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNQILRNVLS 240

Query: 241 YNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDA 300
           YNLWMNACSE+YGVGSVELVY+EML+DKNVQVGWSTLSTLAKVY QAGLV+KAFAAL++A
Sbjct: 241 YNLWMNACSELYGVGSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALREA 300

Query: 301 EKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDI 360
           EKKLS  NRLGYFFLITLYA+LN+KEGVLR+W  SKAVS RPTCANYMCILLCLVKLGDI
Sbjct: 301 EKKLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDI 360

Query: 361 DEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEIL 420
           +EAEKIF+EWE DCRNYDIRVSNVLLGAYVRNGLL++AESLHM  LERGG+PN KTWEIL
Sbjct: 361 NEAEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLDQAESLHMHTLERGGTPNYKTWEIL 420

Query: 421 MEGWVRSQNVARDIDFL-KDFALLKCH 445
           MEG VRSQN+ R I+FL  DFA LKCH
Sbjct: 421 MEGCVRSQNLDRGINFLNNDFAGLKCH 447

BLAST of Spg033869 vs. ExPASy TrEMBL
Match: A0A6J1HF62 (pentatricopeptide repeat-containing protein At5g27460 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462481 PE=4 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 1.4e-203
Identity = 367/447 (82.10%), Postives = 402/447 (89.93%), Query Frame = 0

Query: 1   MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVEGT--ISTVSPNTL 60
           MTRWLGA E+AART+SVMKT+LRSGTGRI+FLL AWRPISS TSPV+ +  IS VSP TL
Sbjct: 1   MTRWLGASEVAARTKSVMKTTLRSGTGRINFLLRAWRPISSHTSPVDSSDIISAVSPKTL 60

Query: 61  QNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALE 120
           Q G D+GNFK++FMK K PRSS+TTVLQSGN EAQRTSI KLRRV +KL K KRF RALE
Sbjct: 61  QKGGDNGNFKQDFMKLKQPRSSVTTVLQSGNSEAQRTSITKLRRVVKKLCKPKRFERALE 120

Query: 121 VLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVK 180
           VLMLMET+N +R+S ADHALRLELTIK+ GLLKAEE+FNQLPNIASQKASSL LLHGYVK
Sbjct: 121 VLMLMETQNKIRMSPADHALRLELTIKVHGLLKAEEHFNQLPNIASQKASSLLLLHGYVK 180

Query: 181 ARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLS 240
            +NTEKAEAFM KL+ LGLVVDPHLYNEMMKLYVATSQ EKVPLVI+DMKQN I+RNVLS
Sbjct: 181 EKNTEKAEAFMAKLKGLGLVVDPHLYNEMMKLYVATSQNEKVPLVIKDMKQNHILRNVLS 240

Query: 241 YNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDA 300
           YNLWMNACSE+YG  SVELVY+EML+DKNVQVGWSTLSTLAKVY QAGLV+KAFAALK A
Sbjct: 241 YNLWMNACSELYGFRSVELVYEEMLSDKNVQVGWSTLSTLAKVYTQAGLVHKAFAALKAA 300

Query: 301 EKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDI 360
           EKKLS  NRLGYFFLITLYA+LN+KEGVLR+W  SKAVS RPTCANYMCILLCLVKLGD+
Sbjct: 301 EKKLSSGNRLGYFFLITLYATLNDKEGVLRVWTASKAVSDRPTCANYMCILLCLVKLGDL 360

Query: 361 DEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEIL 420
           +EAEKIF+EWE DCRNYDIRVSNVLLGAYVRNGLLEKAESLHM  LERGG+PN KTWEIL
Sbjct: 361 NEAEKIFKEWELDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMHTLERGGTPNYKTWEIL 420

Query: 421 MEGWVRSQNVARDIDFLK-DFALLKCH 445
           MEG VRSQN+ R I+FL+ +FA LKCH
Sbjct: 421 MEGCVRSQNLDRGINFLENNFAGLKCH 447

BLAST of Spg033869 vs. ExPASy TrEMBL
Match: A0A6J1BVG4 (pentatricopeptide repeat-containing protein At5g27460-like OS=Momordica charantia OX=3673 GN=LOC111005082 PE=4 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.3e-196
Identity = 355/433 (81.99%), Postives = 385/433 (88.91%), Query Frame = 0

Query: 17  VMKTSLRSGTGRIHFLLEAWRPISSVTSPVE----GTISTVSPNTLQNGDDDGNFKREFM 76
           +M+T LRSG GR+HFLLEAWRPISS+ SP       TIS VSP TL+ G DD NFKRE  
Sbjct: 1   MMRTYLRSGAGRVHFLLEAWRPISSLISPSPVNSFCTISAVSPKTLKYGGDDDNFKRESK 60

Query: 77  KFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALEVLMLMETRNNLRIS 136
           K K PRSS+TTVLQSGNRE QR SI KLR+V +KL KSKRFA ALEVLMLMETRN LR+S
Sbjct: 61  KIKLPRSSVTTVLQSGNRETQRISITKLRKVTKKLCKSKRFASALEVLMLMETRNKLRMS 120

Query: 137 TADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVKARNTEKAEAFMVKL 196
            ADHALRLELTIKL GLL+AEEYF++LPNIASQKASSLPLLHGYVK RNTEKAEAFM+KL
Sbjct: 121 PADHALRLELTIKLHGLLEAEEYFSKLPNIASQKASSLPLLHGYVKDRNTEKAEAFMMKL 180

Query: 197 RDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEIYGV 256
           +DLGLVV+PHLYNEMMKLYVATSQ EKVPLVIRDMKQNQIMRNVLSYNLWMNACSE+YGV
Sbjct: 181 KDLGLVVEPHLYNEMMKLYVATSQNEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEMYGV 240

Query: 257 GSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDAEKKLSPCNRLGYFF 316
           GSVE V+KEML D+NVQVGWSTLSTLAKVYI+AG+V KAF+ALKDAEKKLSPCNRLGYFF
Sbjct: 241 GSVEWVHKEMLKDENVQVGWSTLSTLAKVYIEAGVVEKAFSALKDAEKKLSPCNRLGYFF 300

Query: 317 LITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDIDEAEKIFREWEFDC 376
           LITLYASLNNKEGVLRLWR SKAV GRPTCANYMC+LLCLVKLG+I+EAEK F+EWE  C
Sbjct: 301 LITLYASLNNKEGVLRLWRASKAVIGRPTCANYMCLLLCLVKLGEIEEAEKKFKEWELSC 360

Query: 377 RNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEILMEGWVRSQNVARDI 436
           RNYDIRVSNVLLGAYVRNGLLEKAE LH+R LERGGSPN KTWEILMEGW++ QN+ R I
Sbjct: 361 RNYDIRVSNVLLGAYVRNGLLEKAELLHLRTLERGGSPNYKTWEILMEGWMKRQNMDRAI 420

Query: 437 DFL-KDFALLKCH 445
           D L KD+ LLK H
Sbjct: 421 DSLKKDYDLLKHH 433

BLAST of Spg033869 vs. ExPASy TrEMBL
Match: A0A5A7VI21 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004540 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.1e-192
Identity = 350/438 (79.91%), Postives = 382/438 (87.21%), Query Frame = 0

Query: 1   MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVE--GTISTVSPNTL 60
           MT WL A E+AARTE VMK  LRSGTGRI+FLL+AWR ISS+TSPV+   T S VS   L
Sbjct: 1   MTLWLRALEVAARTEPVMKMPLRSGTGRIYFLLQAWRSISSLTSPVDSLATNSVVSSKIL 60

Query: 61  QNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALE 120
           QNG DDGNFK+EF K K PR S+ TVLQSGN EAQRTSI KLR V +K  K+KRF RAL+
Sbjct: 61  QNGGDDGNFKQEFRKLKLPRRSVNTVLQSGNCEAQRTSITKLRCVVKKFCKTKRFERALK 120

Query: 121 VLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVK 180
            LMLMETR+N R+  A++ALRLELTIK  GLLKAEEYFNQLP IASQKASSLPLLHGYVK
Sbjct: 121 ALMLMETRDNFRMYPAEYALRLELTIKAHGLLKAEEYFNQLPTIASQKASSLPLLHGYVK 180

Query: 181 ARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLS 240
            RNTEKAEAFMVKLRDLGLVV+ HLYNEMMKLYVAT Q EKVPLVI+DMKQNQI RNVLS
Sbjct: 181 ERNTEKAEAFMVKLRDLGLVVNHHLYNEMMKLYVATYQNEKVPLVIKDMKQNQIPRNVLS 240

Query: 241 YNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDA 300
           YNLWMNACS++YGV S+ELV++EMLTDKNVQVGWST+ TLAKVYIQ GLV KAFAALK+A
Sbjct: 241 YNLWMNACSQVYGVRSIELVFEEMLTDKNVQVGWSTMCTLAKVYIQEGLVEKAFAALKEA 300

Query: 301 EKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDI 360
           EKKLSPC RLGYFFLITLYASL +KEGVLR+WR SKAVSG PTCANYMCILLCLVKLG++
Sbjct: 301 EKKLSPCKRLGYFFLITLYASLKDKEGVLRVWRASKAVSGNPTCANYMCILLCLVKLGEM 360

Query: 361 DEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEIL 420
           D+AEK+F+EWEF+CRNYDIRVSNVLLGAYVRNGLLEKAESLH   L RGGSPN KTWEIL
Sbjct: 361 DKAEKVFKEWEFNCRNYDIRVSNVLLGAYVRNGLLEKAESLHRHTLGRGGSPNYKTWEIL 420

Query: 421 MEGWVRS-QNVARDIDFL 436
           MEGWVRS QNV R I+FL
Sbjct: 421 MEGWVRSQQNVDRAINFL 438

BLAST of Spg033869 vs. ExPASy TrEMBL
Match: A0A1S3BBE1 (pentatricopeptide repeat-containing protein At5g27460 OS=Cucumis melo OX=3656 GN=LOC103488093 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.1e-192
Identity = 350/438 (79.91%), Postives = 382/438 (87.21%), Query Frame = 0

Query: 1   MTRWLGAPEIAARTESVMKTSLRSGTGRIHFLLEAWRPISSVTSPVE--GTISTVSPNTL 60
           MT WL A E+AARTE VMK  LRSGTGRI+FLL+AWR ISS+TSPV+   T S VS   L
Sbjct: 1   MTLWLRALEVAARTEPVMKMPLRSGTGRIYFLLQAWRSISSLTSPVDSLATNSVVSSKIL 60

Query: 61  QNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALE 120
           QNG DDGNFK+EF K K PR S+ TVLQSGN EAQRTSI KLR V +K  K+KRF RAL+
Sbjct: 61  QNGGDDGNFKQEFRKLKLPRRSVNTVLQSGNCEAQRTSITKLRCVVKKFCKTKRFERALK 120

Query: 121 VLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVK 180
            LMLMETR+N R+  A++ALRLELTIK  GLLKAEEYFNQLP IASQKASSLPLLHGYVK
Sbjct: 121 ALMLMETRDNFRMYPAEYALRLELTIKAHGLLKAEEYFNQLPTIASQKASSLPLLHGYVK 180

Query: 181 ARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLS 240
            RNTEKAEAFMVKLRDLGLVV+ HLYNEMMKLYVAT Q EKVPLVI+DMKQNQI RNVLS
Sbjct: 181 ERNTEKAEAFMVKLRDLGLVVNHHLYNEMMKLYVATYQNEKVPLVIKDMKQNQIPRNVLS 240

Query: 241 YNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDA 300
           YNLWMNACS++YGV S+ELV++EMLTDKNVQVGWST+ TLAKVYIQ GLV KAFAALK+A
Sbjct: 241 YNLWMNACSQVYGVRSIELVFEEMLTDKNVQVGWSTMCTLAKVYIQEGLVEKAFAALKEA 300

Query: 301 EKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDI 360
           EKKLSPC RLGYFFLITLYASL +KEGVLR+WR SKAVSG PTCANYMCILLCLVKLG++
Sbjct: 301 EKKLSPCKRLGYFFLITLYASLKDKEGVLRVWRASKAVSGNPTCANYMCILLCLVKLGEM 360

Query: 361 DEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEIL 420
           D+AEK+F+EWEF+CRNYDIRVSNVLLGAYVRNGLLEKAESLH   L RGGSPN KTWEIL
Sbjct: 361 DKAEKVFKEWEFNCRNYDIRVSNVLLGAYVRNGLLEKAESLHRHTLGRGGSPNYKTWEIL 420

Query: 421 MEGWVRS-QNVARDIDFL 436
           MEGWVRS QNV R I+FL
Sbjct: 421 MEGWVRSQQNVDRAINFL 438

BLAST of Spg033869 vs. TAIR 10
Match: AT5G27460.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 410.6 bits (1054), Expect = 1.6e-114
Identity = 212/410 (51.71%), Postives = 286/410 (69.76%), Query Frame = 0

Query: 41  SVTSPVEGTISTVSPNTLQNGDDDGNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKL 100
           + T  V   +S+++  +  +   + N  +E ++   PR S+T++LQ         S+ +L
Sbjct: 14  NTTGVVSRLVSSLADGSDTSSVANRNSLKEILRKNGPRRSVTSLLQERIDSGHAVSLSEL 73

Query: 101 RRVAQKLYKSKRFARALEVLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQL- 160
           R ++++L +S R+  AL+++  ME + ++  S  D ALRL+L IK  GL + EEYF +L 
Sbjct: 74  RLISKRLIRSNRYDLALQMMEWMENQKDIEFSVYDIALRLDLIIKTHGLKQGEEYFEKLL 133

Query: 161 ---PNIASQKASSLPLLHGYVKARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQ 220
               ++   K++ LPLL  YVK +  ++AEA M KL  LG +V PH +NEMMKLY A+ Q
Sbjct: 134 HSSVSMRVAKSAYLPLLRAYVKNKMVKEAEALMEKLNGLGFLVTPHPFNEMMKLYEASGQ 193

Query: 221 TEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLS 280
            EKV +V+  MK N+I RNVLSYNLWMNAC E+ GV +VE VYKEM+ DK+V+VGWS+L 
Sbjct: 194 YEKVVMVVSMMKGNKIPRNVLSYNLWMNACCEVSGVAAVETVYKEMVGDKSVEVGWSSLC 253

Query: 281 TLAKVYIQAGLVNKAFAALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAV 340
           TLA VYI++G   KA   L+DAEK L+  NRLGYFFLITLYASL NKEGV+RLW  SK+V
Sbjct: 254 TLANVYIKSGFDEKARLVLEDAEKMLNRSNRLGYFFLITLYASLGNKEGVVRLWEVSKSV 313

Query: 341 SGRPTCANYMCILLCLVKLGDIDEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKA 400
            GR +C NY+C+L  LVK GD++EAE++F EWE  C NYD+RVSNVLLGAYVRNG + KA
Sbjct: 314 CGRISCVNYICVLSSLVKTGDLEEAERVFSEWEAQCFNYDVRVSNVLLGAYVRNGEIRKA 373

Query: 401 ESLHMRILERGGSPNCKTWEILMEGWVRSQNVARDIDFLKDFALL--KCH 445
           ESLH  +LERGG+PN KTWEILMEGWV+ +N+ + ID +    +L  +CH
Sbjct: 374 ESLHGCVLERGGTPNYKTWEILMEGWVKCENMEKAIDAMHQVFVLMRRCH 423

BLAST of Spg033869 vs. TAIR 10
Match: AT1G02150.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 2.0e-64
Identity = 132/364 (36.26%), Postives = 216/364 (59.34%), Query Frame = 0

Query: 75  KSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALEVLMLMETR-NNLRIST 134
           + P     +VL    +  ++ +  +L RV ++L K KR  +ALEV   M  R    R+S 
Sbjct: 77  EKPELGAASVLNQWEKAGRKLTKWELCRVVKELRKYKRANQALEVYDWMNNRGERFRLSA 136

Query: 135 ADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVKARNTEKAEAFMVKLR 194
           +D A++L+L  K++G+  AEE+F QLP     +     LL+ YV+A++ EKAEA +  +R
Sbjct: 137 SDAAIQLDLIGKVRGIPDAEEFFLQLPENFKDRRVYGSLLNAYVRAKSREKAEALLNTMR 196

Query: 195 DLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLSYNLWMNACSEIYGVG 254
           D G  + P  +N MM LY+   + +KV  ++ +MKQ  I  ++ SYN+W+++C  +  V 
Sbjct: 197 DKGYALHPLPFNVMMTLYMNLREYDKVDAMVFEMKQKDIRLDIYSYNIWLSSCGSLGSVE 256

Query: 255 SVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDAEKKLSPCNRLGYFFL 314
            +ELVY++M +D ++   W+T ST+A +YI+ G   KA  AL+  E +++  NR+ Y +L
Sbjct: 257 KMELVYQQMKSDVSIYPNWTTFSTMATMYIKMGETEKAEDALRKVEARITGRNRIPYHYL 316

Query: 315 ITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDIDEAEKIFREWEFDCR 374
           ++LY SL NK+ + R+W   K+V        Y  ++  LV++GDI+ AEK++ EW     
Sbjct: 317 LSLYGSLGNKKELYRVWHVYKSVVPSIPNLGYHALVSSLVRMGDIEGAEKVYEEWLPVKS 376

Query: 375 NYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEILMEGWVRSQNVARDID 434
           +YD R+ N+L+ AYV+N  LE AE L   ++E GG P+  TWEIL  G  R + ++  + 
Sbjct: 377 SYDPRIPNLLMNAYVKNDQLETAEGLFDHMVEMGGKPSSSTWEILAVGHTRKRCISEALT 436

Query: 435 FLKD 438
            L++
Sbjct: 437 CLRN 440

BLAST of Spg033869 vs. TAIR 10
Match: AT1G60770.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 228.0 bits (580), Expect = 1.5e-59
Identity = 121/329 (36.78%), Postives = 188/329 (57.14%), Query Frame = 0

Query: 105 QKLYKSKRFARALEVLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIAS 164
           +KL     +  AL++  +ME R  +  + +D A+ L+L  K + +   E YF  LP  + 
Sbjct: 63  KKLRNRGLYYPALKLSEVMEER-GMNKTVSDQAIHLDLVAKAREITAGENYFVDLPETSK 122

Query: 165 QKASSLPLLHGYVKARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVI 224
            + +   LL+ Y K   TEKAE  + K+++L +      YN +M LY  T +TEKVP +I
Sbjct: 123 TELTYGSLLNCYCKELLTEKAEGLLNKMKELNITPSSMSYNSLMTLYTKTGETEKVPAMI 182

Query: 225 RDMKQNQIMRNVLSYNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQ 284
           +++K   +M +  +YN+WM A +    +  VE V +EM  D  V   W+T S +A +Y+ 
Sbjct: 183 QELKAENVMPDSYTYNVWMRALAATNDISGVERVIEEMNRDGRVAPDWTTYSNMASIYVD 242

Query: 285 AGLVNKAFAALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCAN 344
           AGL  KA  AL++ E K +  +   Y FLITLY  L     V R+WR  +    + +   
Sbjct: 243 AGLSQKAEKALQELEMKNTQRDFTAYQFLITLYGRLGKLTEVYRIWRSLRLAIPKTSNVA 302

Query: 345 YMCILLCLVKLGDIDEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRIL 404
           Y+ ++  LVKL D+  AE +F+EW+ +C  YDIR+ NVL+GAY + GL++KA  L  +  
Sbjct: 303 YLNMIQVLVKLNDLPGAETLFKEWQANCSTYDIRIVNVLIGAYAQEGLIQKANELKEKAP 362

Query: 405 ERGGSPNCKTWEILMEGWVRSQNVARDID 434
            RGG  N KTWEI M+ +V+S ++AR ++
Sbjct: 363 RRGGKLNAKTWEIFMDYYVKSGDMARALE 390

BLAST of Spg033869 vs. TAIR 10
Match: AT4G02820.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 204.1 bits (518), Expect = 2.3e-52
Identity = 124/429 (28.90%), Postives = 225/429 (52.45%), Query Frame = 0

Query: 15  ESVMKTSLRSGTGRIH--FLLEAWRPISSVTSPV--------EGTISTVSPNTLQNGDDD 74
           ++++  S R     IH  F   A   + + T+PV        +G  S     T+  G D 
Sbjct: 3   KNMLVRSARPTLASIHRLFSAAAAATVDTATAPVVKPRSGGGKGGESANKKETVVGGRD- 62

Query: 75  GNFKREFMKFKSPRSSMTTVLQSGNREAQRTSIIKLRRVAQKLYKSKRFARALEVLMLME 134
                  +     + S    ++    E       +L R+ ++L K KR+  ALE+   M 
Sbjct: 63  -TLGGRLLSLVYTKRSAVVTIRKWKEEGHSVRKYELNRIVRELRKIKRYKHALEICEWMV 122

Query: 135 TRNNLRISTADHALRLELTIKLQGLLKAEEYFNQLPNIASQKASSLPLLHGYVKARNTEK 194
            + ++++   D+A+ L+L  K++GL  AE++F  +P+     A+   LLH YV+ + ++K
Sbjct: 123 VQEDIKLQAGDYAVHLDLISKIRGLNSAEKFFEDMPDQMRGHAACTSLLHSYVQNKLSDK 182

Query: 195 AEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTEKVPLVIRDMKQNQIMRNVLSYNLWMN 254
           AEA   K+ + G +     YN M+ +Y++  Q EKVP++I+++K  +   ++++YNLW+ 
Sbjct: 183 AEALFEKMGECGFLKSCLPYNHMLSMYISRGQFEKVPVLIKELK-IRTSPDIVTYNLWLT 242

Query: 255 ACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTLAKVYIQAGLVNKAFAALKDAEKKLSP 314
           A +    V   E VY +   +K +   W T S L  +Y +   V KA  ALK+ EK +S 
Sbjct: 243 AFASGNDVEGAEKVYLKAKEEK-LNPDWVTYSVLTNLYAKTDNVEKARLALKEMEKLVSK 302

Query: 315 CNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSGRPTCANYMCILLCLVKLGDIDEAEKI 374
            NR+ Y  LI+L+A+L +K+GV   W+  K+   +   A Y+ ++  +VKLG+ ++A+ +
Sbjct: 303 KNRVAYASLISLHANLGDKDGVNLTWKKVKSSFKKMNDAEYLSMISAVVKLGEFEQAKGL 362

Query: 375 FREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAESLHMRILERGGSPNCKTWEILMEGWVR 434
           + EWE      D R+ N++L  Y+    +   E  + RI+E+G +P+  TWEIL   +++
Sbjct: 363 YDEWESVSGTGDARIPNLILAEYMNRDEVLLGEKFYERIVEKGINPSYSTWEILTWAYLK 422

BLAST of Spg033869 vs. TAIR 10
Match: AT1G02370.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-50
Identity = 111/321 (34.58%), Postives = 180/321 (56.07%), Query Frame = 0

Query: 100 LRRVAQKLYKSKRFARALEVLMLMETRNNLRISTADHALRLELTIKLQGLLKAEEYFNQL 159
           L R A+ L K +R   A E+   ME R  +  S +DHA+ L+L  K +GL  AE YFN L
Sbjct: 106 LFRCAKTLRKFRRPQHAFEIFDWMEKR-KMTFSVSDHAICLDLIGKTKGLEAAENYFNNL 165

Query: 160 -PNIASQKASSLPLLHGYVKARNTEKAEAFMVKLRDLGLVVDPHLYNEMMKLYVATSQTE 219
            P+  + +++   L++ Y      EKA+A    + +L  V +   +N MM +Y+  SQ E
Sbjct: 166 DPSAKNHQSTYGALMNCYCVELEEEKAKAHFEIMDELNFVNNSLPFNNMMSMYMRLSQPE 225

Query: 220 KVPLVIRDMKQNQIMRNVLSYNLWMNACSEIYGVGSVELVYKEMLTDKNVQVGWSTLSTL 279
           KVP+++  MKQ  I    ++Y++WM +C  +  +  +E +  EM  D   +  W+T S L
Sbjct: 226 KVPVLVDAMKQRGISPCGVTYSIWMQSCGSLNDLDGLEKIIDEMGKDSEAKTTWNTFSNL 285

Query: 280 AKVYIQAGLVNKAFAALKDAEKKLSPCNRLGYFFLITLYASLNNKEGVLRLWRGSKAVSG 339
           A +Y +AGL  KA +ALK  E+K++P NR  + FL++LYA ++    V R+W   K    
Sbjct: 286 AAIYTKAGLYEKADSALKSMEEKMNPNNRDSHHFLMSLYAGISKGPEVYRVWESLKKARP 345

Query: 340 RPTCANYMCILLCLVKLGDIDEAEKIFREWEFDCRNYDIRVSNVLLGAYVRNGLLEKAES 399
                +Y+ +L  + KLGD+D  +KIF EWE  C  YD+R++N+ +  Y++  + E+AE 
Sbjct: 346 EVNNLSYLVMLQAMSKLGDLDGIKKIFTEWESKCWAYDMRLANIAINTYLKGNMYEEAEK 405

Query: 400 LHMRILERGGSPNCKTWEILM 420
           +    +++   P  K  ++LM
Sbjct: 406 ILDGAMKKSKGPFSKARQLLM 425

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022997144.11.3e-20683.00pentatricopeptide repeat-containing protein At5g27460 isoform X1 [Cucurbita maxi... [more]
XP_023545813.12.8e-20683.89pentatricopeptide repeat-containing protein At5g27460 [Cucurbita pepo subsp. pep... [more]
KAG6598713.11.3e-20382.33Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_038885083.11.7e-20382.84pentatricopeptide repeat-containing protein At5g27460 [Benincasa hispida][more]
XP_022961834.12.9e-20382.10pentatricopeptide repeat-containing protein At5g27460 isoform X1 [Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
Q3E9112.2e-11351.71Pentatricopeptide repeat-containing protein At5g27460 OS=Arabidopsis thaliana OX... [more]
Q8LPS62.8e-6336.26Pentatricopeptide repeat-containing protein At1g02150 OS=Arabidopsis thaliana OX... [more]
O227142.1e-5836.78Pentatricopeptide repeat-containing protein At1g60770 OS=Arabidopsis thaliana OX... [more]
Q9SY073.2e-5128.90Pentatricopeptide repeat-containing protein At4g02820, mitochondrial OS=Arabidop... [more]
Q9FZ242.3e-4934.58Pentatricopeptide repeat-containing protein At1g02370, mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1KAM16.2e-20783.00pentatricopeptide repeat-containing protein At5g27460 isoform X1 OS=Cucurbita ma... [more]
A0A6J1HF621.4e-20382.10pentatricopeptide repeat-containing protein At5g27460 isoform X1 OS=Cucurbita mo... [more]
A0A6J1BVG41.3e-19681.99pentatricopeptide repeat-containing protein At5g27460-like OS=Momordica charanti... [more]
A0A5A7VI211.1e-19279.91Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BBE11.1e-19279.91pentatricopeptide repeat-containing protein At5g27460 OS=Cucumis melo OX=3656 GN... [more]
Match NameE-valueIdentityDescription
AT5G27460.11.6e-11451.71Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G02150.12.0e-6436.26Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G60770.11.5e-5936.78Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G02820.12.3e-5228.90Pentatricopeptide repeat (PPR) superfamily protein [more]
AT1G02370.11.6e-5034.58Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 280..441
e-value: 3.4E-21
score: 77.9
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 68..252
e-value: 5.5E-12
score: 47.4
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 381..407
e-value: 3.6E-4
score: 20.6
coord: 345..368
e-value: 0.079
score: 13.2
IPR002885Pentatricopeptide repeatPFAMPF13812PPR_3coord: 193..246
e-value: 0.0013
score: 18.8
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 381..412
e-value: 3.7E-4
score: 18.5
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 376..410
score: 10.018685
NoneNo IPR availablePANTHERPTHR45717:SF13OS02G0796400 PROTEINcoord: 48..442
NoneNo IPR availablePANTHERPTHR45717OS12G0527900 PROTEINcoord: 48..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg033869.1Spg033869.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005515 protein binding