Spg033793 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg033793
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionENTH domain-containing protein
Locationscaffold13: 34100048 .. 34101136 (-)
RNA-Seq ExpressionSpg033793
SyntenySpg033793
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGCTTCTGTGAAAAAATATGCGATGGGCATAGACCAAAACAAGAAGCTCAAGAATCTCATACACGCTCTTAAAGATCAGGCCTCAATAATCAAAGCCACTTTCTCGATTCATCGCCGATCATCTTCCATTAAAGTCGCCGTCGTCCGCGCCACCACCCACGACCCCGGAAACCCCCCTTCCGACGGCCGAGTCGCCGCCGTGCTCGCCCTTGGAAATGACTTCCGTTCCTCAACTGCATTCGCCTGCATCGAAGCGCTTATGCAGCGTCTTCACACAACTTCCAGCGCCGCCGTGGCCATGAAATCGCTTTTCACTCTGCATATAATCGTAATTCGAGGTCCGTTCAACCTGAGGGATCAAGTGGCGTTTTGCCCCTTTTACGGAGGGAGAAACTTTCTAAACTTGTCCGCGTTTCGCGACGTATCGGACTCGGAGATGAGCGACTTGTCGTCTTGGGTGAGATGGTACGCGGCCGTCGTGGAGCATAACGTGATCGTCCAGAGGAAATTGGATGGGATTCTGTATCTGCGTTCAAGAAATTGCGAAATCGAAGATAAAGAAGGGAGGAAGGTTGATTTATCGGGGGAATTGGACGTTCTTGTGGGTTTTGTGGAGCGGATTTGCGAAGTTCCCGAGTCGTTGCATCTTCAGAGGAACGATTTGGTTTACGAGGTGGTGAGATTGGTTATGGAGAATTACAGGTTGGTTCAACGGGAGATTTGGGTCCGAGTTAAGGAAATCGGAGACAGAGTGGAGAGTTTGAGTCTGGACGAGTTGACTCAGTTGGTGGGTATCTTGACGCGGTTCGAGAATTGCAGAAGGAAACTCAGTGTGTTGTTTGTGAACAGAGGGAAGAACGAGGATTTGTGGGAATTGGTGAAGAACACGAAAGGGAAACTGGTGGAGCAGAAGGAAAGGAAAGAGGAGAAGAGGATGATCGTGGTGGAGATGAGAGCGGACTCGGTTGAGTCGACTCGGTTCTGGAACCCGTTTGTTGAACCGGGTCAGTTGCTGTGGGTCCCAACCGGTGATGGACCGCTGGGCCCGGCTCTGCTTCCACTGACCGTTTCAACGGTAGGATAG

mRNA sequence

ATGATTGCTTCTGTGAAAAAATATGCGATGGGCATAGACCAAAACAAGAAGCTCAAGAATCTCATACACGCTCTTAAAGATCAGGCCTCAATAATCAAAGCCACTTTCTCGATTCATCGCCGATCATCTTCCATTAAAGTCGCCGTCGTCCGCGCCACCACCCACGACCCCGGAAACCCCCCTTCCGACGGCCGAGTCGCCGCCGTGCTCGCCCTTGGAAATGACTTCCGTTCCTCAACTGCATTCGCCTGCATCGAAGCGCTTATGCAGCGTCTTCACACAACTTCCAGCGCCGCCGTGGCCATGAAATCGCTTTTCACTCTGCATATAATCGTAATTCGAGGTCCGTTCAACCTGAGGGATCAAGTGGCGTTTTGCCCCTTTTACGGAGGGAGAAACTTTCTAAACTTGTCCGCGTTTCGCGACGTATCGGACTCGGAGATGAGCGACTTGTCGTCTTGGGTGAGATGGTACGCGGCCGTCGTGGAGCATAACGTGATCGTCCAGAGGAAATTGGATGGGATTCTGTATCTGCGTTCAAGAAATTGCGAAATCGAAGATAAAGAAGGGAGGAAGGTTGATTTATCGGGGGAATTGGACGTTCTTGTGGGTTTTGTGGAGCGGATTTGCGAAGTTCCCGAGTCGTTGCATCTTCAGAGGAACGATTTGGTTTACGAGGTGGTGAGATTGGTTATGGAGAATTACAGGTTGGTTCAACGGGAGATTTGGGTCCGAGTTAAGGAAATCGGAGACAGAGTGGAGAGTTTGAGTCTGGACGAGTTGACTCAGTTGGTGGGTATCTTGACGCGGTTCGAGAATTGCAGAAGGAAACTCAGTGTGTTGTTTGTGAACAGAGGGAAGAACGAGGATTTGTGGGAATTGGTGAAGAACACGAAAGGGAAACTGGTGGAGCAGAAGGAAAGGAAAGAGGAGAAGAGGATGATCGTGGTGGAGATGAGAGCGGACTCGGTTGAGTCGACTCGGTTCTGGAACCCGTTTGTTGAACCGGGTCAGTTGCTGTGGGTCCCAACCGGTGATGGACCGCTGGGCCCGGCTCTGCTTCCACTGACCGTTTCAACGGTAGGATAG

Coding sequence (CDS)

ATGATTGCTTCTGTGAAAAAATATGCGATGGGCATAGACCAAAACAAGAAGCTCAAGAATCTCATACACGCTCTTAAAGATCAGGCCTCAATAATCAAAGCCACTTTCTCGATTCATCGCCGATCATCTTCCATTAAAGTCGCCGTCGTCCGCGCCACCACCCACGACCCCGGAAACCCCCCTTCCGACGGCCGAGTCGCCGCCGTGCTCGCCCTTGGAAATGACTTCCGTTCCTCAACTGCATTCGCCTGCATCGAAGCGCTTATGCAGCGTCTTCACACAACTTCCAGCGCCGCCGTGGCCATGAAATCGCTTTTCACTCTGCATATAATCGTAATTCGAGGTCCGTTCAACCTGAGGGATCAAGTGGCGTTTTGCCCCTTTTACGGAGGGAGAAACTTTCTAAACTTGTCCGCGTTTCGCGACGTATCGGACTCGGAGATGAGCGACTTGTCGTCTTGGGTGAGATGGTACGCGGCCGTCGTGGAGCATAACGTGATCGTCCAGAGGAAATTGGATGGGATTCTGTATCTGCGTTCAAGAAATTGCGAAATCGAAGATAAAGAAGGGAGGAAGGTTGATTTATCGGGGGAATTGGACGTTCTTGTGGGTTTTGTGGAGCGGATTTGCGAAGTTCCCGAGTCGTTGCATCTTCAGAGGAACGATTTGGTTTACGAGGTGGTGAGATTGGTTATGGAGAATTACAGGTTGGTTCAACGGGAGATTTGGGTCCGAGTTAAGGAAATCGGAGACAGAGTGGAGAGTTTGAGTCTGGACGAGTTGACTCAGTTGGTGGGTATCTTGACGCGGTTCGAGAATTGCAGAAGGAAACTCAGTGTGTTGTTTGTGAACAGAGGGAAGAACGAGGATTTGTGGGAATTGGTGAAGAACACGAAAGGGAAACTGGTGGAGCAGAAGGAAAGGAAAGAGGAGAAGAGGATGATCGTGGTGGAGATGAGAGCGGACTCGGTTGAGTCGACTCGGTTCTGGAACCCGTTTGTTGAACCGGGTCAGTTGCTGTGGGTCCCAACCGGTGATGGACCGCTGGGCCCGGCTCTGCTTCCACTGACCGTTTCAACGGTAGGATAG

Protein sequence

MIASVKKYAMGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAVLALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFYGGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKEGRKVDLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVKEIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKERKEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG
Homology
BLAST of Spg033793 vs. NCBI nr
Match: XP_038884022.1 (putative clathrin assembly protein At4g40080 [Benincasa hispida])

HSP 1 Score: 586.6 bits (1511), Expect = 1.4e-163
Identity = 303/354 (85.59%), Postives = 323/354 (91.24%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           M IDQNKKLKNL HALKD+ASIIKAT SI RRSSSIKVAVVRATTH   NPPSD RVAAV
Sbjct: 1   MAIDQNKKLKNLTHALKDKASIIKATLSIPRRSSSIKVAVVRATTHGSRNPPSDARVAAV 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LALGNDFRSSTAFACIEALM+RLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVA+ P Y
Sbjct: 61  LALGNDFRSSTAFACIEALMERLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAYFPCY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEI-EDK 189
           GGRNFLNLS FRDVSDSEM+DLSSWVRWYA VVE NVIV RKLD ILY RSRNCEI E++
Sbjct: 121 GGRNFLNLSTFRDVSDSEMNDLSSWVRWYAGVVESNVIVDRKLDRILYFRSRNCEIVEEQ 180

Query: 190 EGRKVDLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVKE 249
             RK+D+  EL+VLVGFVERICEVPESL+LQ+ DLVYEVVRLV+ENYRLVQREIWVRVKE
Sbjct: 181 RKRKIDVPEELEVLVGFVERICEVPESLYLQKKDLVYEVVRLVLENYRLVQREIWVRVKE 240

Query: 250 IGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKER 309
           IGDRVESLSLDELT+LVGI+TR ENCRRKLSVLFVNRGKNE+ WELVK TKGKL E+K  
Sbjct: 241 IGDRVESLSLDELTELVGIMTRLENCRRKLSVLFVNRGKNEEFWELVKITKGKLAEKKRM 300

Query: 310 KEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
           KEEKRMI+VEM+A+S ESTR WNPFVEPGQLLWVP GDGP+GPALLPLTVSTVG
Sbjct: 301 KEEKRMIMVEMKANSGESTRLWNPFVEPGQLLWVPAGDGPMGPALLPLTVSTVG 354

BLAST of Spg033793 vs. NCBI nr
Match: XP_022131457.1 (putative clathrin assembly protein At4g40080 [Momordica charantia])

HSP 1 Score: 582.8 bits (1501), Expect = 2.1e-162
Identity = 297/355 (83.66%), Postives = 325/355 (91.55%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           MGIDQ KKLKNLI ALKD+ASIIKATFS HRRSSSIK+AVVRATTHDP NPPSD R+AAV
Sbjct: 1   MGIDQKKKLKNLIDALKDKASIIKATFSTHRRSSSIKLAVVRATTHDPSNPPSDRRLAAV 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LALGNDF  STA ACI+ +M RLHTTSSA VAMKSLFTLHI+VIRGPF+LRDQV FCP+Y
Sbjct: 61  LALGNDFGRSTAIACIQTIMDRLHTTSSAVVAMKSLFTLHIVVIRGPFDLRDQVVFCPYY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKE 189
           GGRNFLNLSAFRDVSDSEMSDLSSWVRWYA VVEHNVIV+R+LD ILYLRS NC+IEDK+
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAGVVEHNVIVRRRLDRILYLRSGNCKIEDKQ 180

Query: 190 GR--KVDLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVK 249
           G+  ++DL GELDVLVGFVE ICE P+SLHLQ+N++VYEVVRLV+ENYRLVQREI VRV+
Sbjct: 181 GKISELDLWGELDVLVGFVEGICEFPDSLHLQKNEMVYEVVRLVLENYRLVQREISVRVR 240

Query: 250 EIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKE 309
            IGDR +SLSLDELTQLV ILTRFENCRRKL+VLFVNR KNEDLWELVKNTK KLVEQK+
Sbjct: 241 GIGDRADSLSLDELTQLVVILTRFENCRRKLTVLFVNRAKNEDLWELVKNTKAKLVEQKQ 300

Query: 310 RKEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
            KEEKRMI+VE+RA+SVE TR WNPFVEPGQLLWVP+GD PLGPALLPLTVSTVG
Sbjct: 301 MKEEKRMIMVEIRAESVELTRLWNPFVEPGQLLWVPSGDEPLGPALLPLTVSTVG 355

BLAST of Spg033793 vs. NCBI nr
Match: XP_008445571.1 (PREDICTED: putative clathrin assembly protein At4g40080 [Cucumis melo] >KAA0065124.1 putative clathrin assembly protein [Cucumis melo var. makuwa])

HSP 1 Score: 565.1 bits (1455), Expect = 4.4e-157
Identity = 297/355 (83.66%), Postives = 318/355 (89.58%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           M I Q+KKLKNL HALKD+ASIIKA FSI+RRSSSIKVAVVRATTH   NPPSD RVAAV
Sbjct: 1   MAIHQSKKLKNLTHALKDKASIIKANFSINRRSSSIKVAVVRATTHGARNPPSDARVAAV 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LALGNDFRSSTAFACIEALM RLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQV+F P Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKE 189
           GGRNFLNLSAFRDVSDSEM+DLSSWVRWYA VVEHNVIV RKLD ILY RSRNCEI D+ 
Sbjct: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI-DEG 180

Query: 190 GR--KVDLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVK 249
           GR  KVDL+ EL VLVGFVERICEVPESLHLQ+ DLVYEVVRLV+ENYRLVQREIWVRVK
Sbjct: 181 GRKWKVDLAEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLENYRLVQREIWVRVK 240

Query: 250 EIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKE 309
           EIG+RVE LS+DEL++LVGIL R ENCR K+SVLFVNRGKNE+ WELVK TKGK+ E+K 
Sbjct: 241 EIGERVERLSVDELSELVGILIRLENCRWKVSVLFVNRGKNEEFWELVKITKGKVAEKKR 300

Query: 310 RKEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
            KEEKRM++V    DSVESTR WNPFVEPGQL+WVP GDGP+GPALLPLTVSTVG
Sbjct: 301 LKEEKRMVMV---VDSVESTRLWNPFVEPGQLVWVPGGDGPMGPALLPLTVSTVG 351

BLAST of Spg033793 vs. NCBI nr
Match: XP_004152749.1 (putative clathrin assembly protein At4g40080 [Cucumis sativus] >KGN62720.1 hypothetical protein Csa_022647 [Cucumis sativus])

HSP 1 Score: 545.4 bits (1404), Expect = 3.6e-151
Identity = 291/355 (81.97%), Postives = 315/355 (88.73%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           M I QNKKL NL+HALKD+AS+IKATFSI+RRSSSIKVAVVRATTH   NPPSD RV+AV
Sbjct: 1   MAIHQNKKLNNLLHALKDKASLIKATFSINRRSSSIKVAVVRATTHGARNPPSDARVSAV 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LALGNDFRSSTAFACIEALM RLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQV+F P Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKE 189
           GGRNFLNLSAFRDVSDSEMSDLSSWVRWYA VVEHNVIV RKLD ILY RSRNCEI D++
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI-DED 180

Query: 190 GR--KVDLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVK 249
           GR  KVDLS EL VLVGFVERICEVPESLHLQ+ DLVYEVVRLV++NYRLVQ+EIWVRVK
Sbjct: 181 GRKGKVDLSEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLQNYRLVQKEIWVRVK 240

Query: 250 EIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKE 309
           EIG+RVE LS+DEL++LVGILTR ENCR K+SVLFVNRGK+E+ WELVK T+GKL E+K 
Sbjct: 241 EIGERVERLSVDELSELVGILTRLENCRWKVSVLFVNRGKSEEFWELVKKTRGKLGEKKR 300

Query: 310 RKEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
            KEEKRMI+V    +SVESTR  NPFVEPGQL+WVP      GPALLPLTVSTVG
Sbjct: 301 LKEEKRMIMV---VESVESTRLRNPFVEPGQLMWVPG-----GPALLPLTVSTVG 346

BLAST of Spg033793 vs. NCBI nr
Match: KAG6598708.1 (putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 485.7 bits (1249), Expect = 3.4e-133
Identity = 263/354 (74.29%), Postives = 287/354 (81.07%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           M IDQ KK KNLI A KDQASIIKATFSIHRRSSSIKVAVVRATTH   NPPSD R+AA+
Sbjct: 1   MAIDQTKKFKNLIDAFKDQASIIKATFSIHRRSSSIKVAVVRATTHGARNPPSDARLAAL 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LA GNDFRSSTAF CI+ALM+RLHTT+SAAVAMKSLFTLHII IRGPFNLR +VAF P+Y
Sbjct: 61  LAFGNDFRSSTAFLCIQALMERLHTTTSAAVAMKSLFTLHIIAIRGPFNLRGEVAFSPYY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKE 189
           GGRNFLNLSAFRDVSDSEMS+LS WVRWYA VVEHN    RKLD ILY RSRN EI + +
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSELSCWVRWYAGVVEHN----RKLDRILYFRSRNPEIVEGK 180

Query: 190 GRKV-DLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVKE 249
            RK+ +L  ELDVLVGF ERI EVPESLH+Q++DLVYEVVRLV+E+YRLVQREIWVRV E
Sbjct: 181 DRKIPELLEELDVLVGFSERISEVPESLHVQKSDLVYEVVRLVLESYRLVQREIWVRVNE 240

Query: 250 IGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKER 309
           IG+RVE +S DELT+ V ILTR ENCRRK+SVLFVNRGKNE+LWELV  TKGKLVE++ R
Sbjct: 241 IGNRVEWVSRDELTESVEILTRMENCRRKVSVLFVNRGKNEELWELVTCTKGKLVERRRR 300

Query: 310 KEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
                     M     ESTR WNPFVEPG L        P GPA LPLTVSTVG
Sbjct: 301 ----------MTTTMTESTRLWNPFVEPGSL-------RPFGPAFLPLTVSTVG 333

BLAST of Spg033793 vs. ExPASy Swiss-Prot
Match: Q8L936 (Putative clathrin assembly protein At4g40080 OS=Arabidopsis thaliana OX=3702 GN=At4g40080 PE=2 SV=2)

HSP 1 Score: 178.7 bits (452), Expect = 1.2e-43
Identity = 118/325 (36.31%), Postives = 178/325 (54.77%), Query Frame = 0

Query: 20  NLIHALKDQASIIKATF---SIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAVLALGNDF 79
           +LI  +KD+AS  KA     +   ++ S  ++V+RATTHDP  PP +  +A +L+ G   
Sbjct: 9   DLIGRIKDKASQSKAALVSSNTKSKTLSFHLSVLRATTHDPSTPPGNRHLAVILSAGTGS 68

Query: 80  RSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFYGGRNFLN 139
           R +TA + +E++M+RLHTT  A VA+KSL  +H IV  G F L+DQ++  P  GGRN+L 
Sbjct: 69  R-ATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSVFPASGGRNYLK 128

Query: 140 LSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKEGRKV--- 199
           LSAFRD     M +LSSWVRWYA  +EH +   R +    ++ S +  I  +E  ++   
Sbjct: 129 LSAFRDEKSPLMWELSSWVRWYALYLEHLLSTSRIMG--FFISSTSSTIHKEEYEEMVSS 188

Query: 200 ----DLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVKEI 259
               DL  E+D LVG +E  C++P+        L  ++ +LV E+Y     E++ R  E 
Sbjct: 189 LTNSDLLREIDALVGLLEEACKIPDLPFSGGKSLADKITQLVGEDYVSSINELYTRFNEF 248

Query: 260 GDRVESLSLDELTQLVGILTRFENCRRKLSVLF---VNRGKNEDLWELVKNTKGKL--VE 319
            +R  +LS  +  +LV  L R E+C+ +LS +      RG  +  W LV   KG +  +E
Sbjct: 249 KERSNTLSFGDTIELVCALKRLESCKERLSEICHGNWKRGWIDGFWGLVLEVKGIIGNLE 308

Query: 320 QKERKEEKRMIVVEMRADSVESTRF 330
               + EK ++    R    ES RF
Sbjct: 309 DNYGQIEKSIVGFGKRDKGYESARF 330

BLAST of Spg033793 vs. ExPASy Swiss-Prot
Match: Q8H0W9 (Putative clathrin assembly protein At5g10410 OS=Arabidopsis thaliana OX=3702 GN=At5g10410 PE=2 SV=2)

HSP 1 Score: 105.5 bits (262), Expect = 1.3e-21
Identity = 97/313 (30.99%), Postives = 157/313 (50.16%), Query Frame = 0

Query: 20  NLIHALKDQASIIKATFSIHRRSSSIK---VAVVRATTHDPGNPPSDGRVAAVLALGNDF 79
           ++I   KD+ASI KA       S+++K   +A++++TT  P  PP+   V+AV++  N  
Sbjct: 7   HIIGKFKDKASIGKARLVHSFGSTAVKYIHLALLKSTTRTPNKPPNSDYVSAVISYSNSR 66

Query: 80  RSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFYGGRNFLN 139
            +  AF+   A + RL  T +A VA KSL  +H ++     + RD+  F     GRN L 
Sbjct: 67  YAPAAFS---AALWRLRVTKNAIVATKSLIVIHKLI----KSSRDK--FEGLGHGRNNLK 126

Query: 140 LSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGI-LYLRSRNCEIEDKEGRKVDL 199
           L+ F D S +   +LS W+RWY   ++    V + L      L +   ++E+K+      
Sbjct: 127 LNEFSDKSSNLTLELSQWIRWYGQYLDRLSWVPKVLGSFPNLLVNPKDKVEEKDRVSSYQ 186

Query: 200 SG----ELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVKEIGD 259
           +G    + D LV F E IC  PE   + +N +V E+  LV+E+Y  + R + VR++ + +
Sbjct: 187 TGYIIRQTDSLVSFFEHICTRPEIPPMFQNKIVDEIRELVIEDYFKIVRLVMVRLQVLFE 246

Query: 260 R--------VESLSLDELTQLVGILTRFENCRRKLSVLFVN-RGKNEDLWELVKNTKGKL 316
           R        +  L L++ + L   L R   C+  LS LF   R   +D W LV+  K   
Sbjct: 247 RLIKPGVKPIGDLGLNDFSLL---LVRLVECKESLSGLFWRCRRLADDFWCLVEMLKA-- 303

BLAST of Spg033793 vs. ExPASy Swiss-Prot
Match: Q9FKQ2 (Putative clathrin assembly protein At5g65370 OS=Arabidopsis thaliana OX=3702 GN=At5g65370 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.3e-15
Identity = 91/310 (29.35%), Postives = 144/310 (46.45%), Query Frame = 0

Query: 17  KLKNLIHALKDQASIIKATFSIHRRSS----SIKVAVVRATTHDPGNPPSDGRVAAVLAL 76
           KL  L   LKD+AS +K    +H  SS    +I +A+++AT+H   NPPSD  V      
Sbjct: 3   KLATLNGILKDEASQMKLNV-VHLCSSVNAKTIDLALLKATSHTSNNPPSDKYVT----- 62

Query: 77  GNDFRSSTAFAC-----IEALMQRLHTTSSAAVAMKSLFTLHIIV-----IRGPFNLRDQ 136
              F  ST   C     ++A++ RL  T+   VA K L  LH +V       G  +LR+ 
Sbjct: 63  ---FLQSTIDTCYGPDTVDAILHRLRVTTDVCVAAKCLILLHKMVKSESGYNGEDSLRNN 122

Query: 137 V---AFCPFYGGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLR 196
           +         GG N L L+     S     +L+ WV+WY   ++  + +   L     ++
Sbjct: 123 INHRTLIYTQGGSN-LKLNDLNVNSSRFTRELTPWVQWYKQYLDCYLSIAEVLGITPNIK 182

Query: 197 SRNCEIEDK--EGRKVD------LSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLV 256
            +N   EDK  E ++V       +  ++D LV   E I + P++   + N +V E+  L+
Sbjct: 183 EKN---EDKRLETQRVSSYPMDCILKQIDFLVELFEHISDRPKAPQSKLNKIVIEMTELM 242

Query: 257 MENYRLVQREIWVRVKEIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKN--E 300
           +++Y    R + +R +E+  RV      +  +LV +L + ENC+  LS  F  R K    
Sbjct: 243 VQDYFSAIRLMRIRFEELNVRVA-----KPNELVPVLEKLENCKEGLSE-FSWRSKYLIA 293

BLAST of Spg033793 vs. ExPASy Swiss-Prot
Match: Q8GX47 (Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=At4g02650 PE=2 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 2.6e-11
Identity = 54/155 (34.84%), Postives = 86/155 (55.48%), Query Frame = 0

Query: 15  NKKLKNLIHALKDQASIIKATFSIHRRSSS---IKVAVVRATTHDPGNPPSDGRVAAVLA 74
           + KLK  I A+KDQ S+  A   +  RSSS   +++AVV+AT HD   P  D  +  +L 
Sbjct: 3   SSKLKRAIGAVKDQTSVGLA--KVGGRSSSLTELEIAVVKATRHD-DYPAEDKYIREILC 62

Query: 75  LGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFYGG 134
           L +  R+  + AC+  L +RL+ T + +VA+K+L  +  ++  G      ++ F    G 
Sbjct: 63  LTSYSRNYVS-ACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGT 122

Query: 135 RNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNV 167
           R  LN+S FRD S S+  D S++VR YA  ++  +
Sbjct: 123 R-LLNMSDFRDASQSDSWDYSAFVRTYALYLDERL 152

BLAST of Spg033793 vs. ExPASy Swiss-Prot
Match: Q9SA65 (Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=At1g03050 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.9e-09
Identity = 51/155 (32.90%), Postives = 82/155 (52.90%), Query Frame = 0

Query: 15  NKKLKNLIHALKDQASIIKATFSIHRRSSSIK---VAVVRATTHDPGNPPSDGRVAAVLA 74
           + K K  I A+KDQ S+  A   ++ RS+S+    VA+V+AT H+   P  +  +  +L+
Sbjct: 3   SSKFKRAIGAVKDQTSVGLA--KVNGRSASLSELDVAIVKATRHEE-FPAEEKYIREILS 62

Query: 75  LGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFYGG 134
           L   +  S   AC+  L +RL+ T    VA+K+L  +  ++  G      ++ F    G 
Sbjct: 63  L-TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGT 122

Query: 135 RNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNV 167
           R  LN+S FRDVS S   D S++VR YA  ++  +
Sbjct: 123 R-LLNMSDFRDVSRSNSWDYSAFVRTYALYLDERL 152

BLAST of Spg033793 vs. ExPASy TrEMBL
Match: A0A6J1BR25 (putative clathrin assembly protein At4g40080 OS=Momordica charantia OX=3673 GN=LOC111004659 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 9.9e-163
Identity = 297/355 (83.66%), Postives = 325/355 (91.55%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           MGIDQ KKLKNLI ALKD+ASIIKATFS HRRSSSIK+AVVRATTHDP NPPSD R+AAV
Sbjct: 1   MGIDQKKKLKNLIDALKDKASIIKATFSTHRRSSSIKLAVVRATTHDPSNPPSDRRLAAV 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LALGNDF  STA ACI+ +M RLHTTSSA VAMKSLFTLHI+VIRGPF+LRDQV FCP+Y
Sbjct: 61  LALGNDFGRSTAIACIQTIMDRLHTTSSAVVAMKSLFTLHIVVIRGPFDLRDQVVFCPYY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKE 189
           GGRNFLNLSAFRDVSDSEMSDLSSWVRWYA VVEHNVIV+R+LD ILYLRS NC+IEDK+
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAGVVEHNVIVRRRLDRILYLRSGNCKIEDKQ 180

Query: 190 GR--KVDLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVK 249
           G+  ++DL GELDVLVGFVE ICE P+SLHLQ+N++VYEVVRLV+ENYRLVQREI VRV+
Sbjct: 181 GKISELDLWGELDVLVGFVEGICEFPDSLHLQKNEMVYEVVRLVLENYRLVQREISVRVR 240

Query: 250 EIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKE 309
            IGDR +SLSLDELTQLV ILTRFENCRRKL+VLFVNR KNEDLWELVKNTK KLVEQK+
Sbjct: 241 GIGDRADSLSLDELTQLVVILTRFENCRRKLTVLFVNRAKNEDLWELVKNTKAKLVEQKQ 300

Query: 310 RKEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
            KEEKRMI+VE+RA+SVE TR WNPFVEPGQLLWVP+GD PLGPALLPLTVSTVG
Sbjct: 301 MKEEKRMIMVEIRAESVELTRLWNPFVEPGQLLWVPSGDEPLGPALLPLTVSTVG 355

BLAST of Spg033793 vs. ExPASy TrEMBL
Match: A0A5A7VCW1 (Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004590 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.1e-157
Identity = 297/355 (83.66%), Postives = 318/355 (89.58%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           M I Q+KKLKNL HALKD+ASIIKA FSI+RRSSSIKVAVVRATTH   NPPSD RVAAV
Sbjct: 1   MAIHQSKKLKNLTHALKDKASIIKANFSINRRSSSIKVAVVRATTHGARNPPSDARVAAV 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LALGNDFRSSTAFACIEALM RLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQV+F P Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKE 189
           GGRNFLNLSAFRDVSDSEM+DLSSWVRWYA VVEHNVIV RKLD ILY RSRNCEI D+ 
Sbjct: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI-DEG 180

Query: 190 GR--KVDLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVK 249
           GR  KVDL+ EL VLVGFVERICEVPESLHLQ+ DLVYEVVRLV+ENYRLVQREIWVRVK
Sbjct: 181 GRKWKVDLAEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLENYRLVQREIWVRVK 240

Query: 250 EIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKE 309
           EIG+RVE LS+DEL++LVGIL R ENCR K+SVLFVNRGKNE+ WELVK TKGK+ E+K 
Sbjct: 241 EIGERVERLSVDELSELVGILIRLENCRWKVSVLFVNRGKNEEFWELVKITKGKVAEKKR 300

Query: 310 RKEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
            KEEKRM++V    DSVESTR WNPFVEPGQL+WVP GDGP+GPALLPLTVSTVG
Sbjct: 301 LKEEKRMVMV---VDSVESTRLWNPFVEPGQLVWVPGGDGPMGPALLPLTVSTVG 351

BLAST of Spg033793 vs. ExPASy TrEMBL
Match: A0A1S3BDW7 (putative clathrin assembly protein At4g40080 OS=Cucumis melo OX=3656 GN=LOC103488553 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.1e-157
Identity = 297/355 (83.66%), Postives = 318/355 (89.58%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           M I Q+KKLKNL HALKD+ASIIKA FSI+RRSSSIKVAVVRATTH   NPPSD RVAAV
Sbjct: 1   MAIHQSKKLKNLTHALKDKASIIKANFSINRRSSSIKVAVVRATTHGARNPPSDARVAAV 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LALGNDFRSSTAFACIEALM RLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQV+F P Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKE 189
           GGRNFLNLSAFRDVSDSEM+DLSSWVRWYA VVEHNVIV RKLD ILY RSRNCEI D+ 
Sbjct: 121 GGRNFLNLSAFRDVSDSEMNDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI-DEG 180

Query: 190 GR--KVDLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVK 249
           GR  KVDL+ EL VLVGFVERICEVPESLHLQ+ DLVYEVVRLV+ENYRLVQREIWVRVK
Sbjct: 181 GRKWKVDLAEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLENYRLVQREIWVRVK 240

Query: 250 EIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKE 309
           EIG+RVE LS+DEL++LVGIL R ENCR K+SVLFVNRGKNE+ WELVK TKGK+ E+K 
Sbjct: 241 EIGERVERLSVDELSELVGILIRLENCRWKVSVLFVNRGKNEEFWELVKITKGKVAEKKR 300

Query: 310 RKEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
            KEEKRM++V    DSVESTR WNPFVEPGQL+WVP GDGP+GPALLPLTVSTVG
Sbjct: 301 LKEEKRMVMV---VDSVESTRLWNPFVEPGQLVWVPGGDGPMGPALLPLTVSTVG 351

BLAST of Spg033793 vs. ExPASy TrEMBL
Match: A0A0A0LLA1 (ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369220 PE=4 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 1.8e-151
Identity = 291/355 (81.97%), Postives = 315/355 (88.73%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           M I QNKKL NL+HALKD+AS+IKATFSI+RRSSSIKVAVVRATTH   NPPSD RV+AV
Sbjct: 1   MAIHQNKKLNNLLHALKDKASLIKATFSINRRSSSIKVAVVRATTHGARNPPSDARVSAV 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LALGNDFRSSTAFACIEALM RLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQV+F P Y
Sbjct: 61  LALGNDFRSSTAFACIEALMNRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVSFFPSY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKE 189
           GGRNFLNLSAFRDVSDSEMSDLSSWVRWYA VVEHNVIV RKLD ILY RSRNCEI D++
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAGVVEHNVIVDRKLDRILYFRSRNCEI-DED 180

Query: 190 GR--KVDLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVK 249
           GR  KVDLS EL VLVGFVERICEVPESLHLQ+ DLVYEVVRLV++NYRLVQ+EIWVRVK
Sbjct: 181 GRKGKVDLSEELVVLVGFVERICEVPESLHLQKKDLVYEVVRLVLQNYRLVQKEIWVRVK 240

Query: 250 EIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKE 309
           EIG+RVE LS+DEL++LVGILTR ENCR K+SVLFVNRGK+E+ WELVK T+GKL E+K 
Sbjct: 241 EIGERVERLSVDELSELVGILTRLENCRWKVSVLFVNRGKSEEFWELVKKTRGKLGEKKR 300

Query: 310 RKEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
            KEEKRMI+V    +SVESTR  NPFVEPGQL+WVP      GPALLPLTVSTVG
Sbjct: 301 LKEEKRMIMV---VESVESTRLRNPFVEPGQLMWVPG-----GPALLPLTVSTVG 346

BLAST of Spg033793 vs. ExPASy TrEMBL
Match: A0A6J1K8Z0 (putative clathrin assembly protein At4g40080 OS=Cucurbita maxima OX=3661 GN=LOC111492184 PE=4 SV=1)

HSP 1 Score: 485.3 bits (1248), Expect = 2.2e-133
Identity = 265/354 (74.86%), Postives = 291/354 (82.20%), Query Frame = 0

Query: 10  MGIDQNKKLKNLIHALKDQASIIKATFSIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAV 69
           M IDQ KKLKNLI A KDQASIIKATFSIHRRSSSIKVAVVRATTH   NPPSD R+AA+
Sbjct: 1   MAIDQTKKLKNLIDAFKDQASIIKATFSIHRRSSSIKVAVVRATTHGARNPPSDARLAAL 60

Query: 70  LALGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFY 129
           LALGNDFRSSTAF CI+ALM+RLHTT+SAAVAMKSLFTLHIIVIRGPFNLRD+VAF P+Y
Sbjct: 61  LALGNDFRSSTAFVCIQALMERLHTTTSAAVAMKSLFTLHIIVIRGPFNLRDEVAFSPYY 120

Query: 130 GGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKE 189
           GGRNFLNLSAFRDVSDSEMS+LS WVRWYA VVEH     RKLD ILY RSRN EI + +
Sbjct: 121 GGRNFLNLSAFRDVSDSEMSELSCWVRWYAGVVEH----IRKLDWILYFRSRNPEIVEGK 180

Query: 190 GRKV-DLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVKE 249
            RK+ +L  ELDVL+GF+ERI EVPESLH+Q++DLVYEVVRLV+E+YRLVQREI VRV E
Sbjct: 181 DRKIPELLEELDVLLGFLERISEVPESLHVQKSDLVYEVVRLVLESYRLVQREIRVRVNE 240

Query: 250 IGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKNEDLWELVKNTKGKLVEQKER 309
           IG+RVE LS DELT+ V ILTR ENCRRK+SVLFVNRGKN++LWELV  TKGKLVE++ R
Sbjct: 241 IGNRVEWLSRDELTESVEILTRMENCRRKVSVLFVNRGKNDELWELVTCTKGKLVERRRR 300

Query: 310 KEEKRMIVVEMRADSVESTRFWNPFVEPGQLLWVPTGDGPLGPALLPLTVSTVG 363
                           ESTR WNPFVEPG L        PLGPALLPLTVSTVG
Sbjct: 301 -----------MTTMTESTRLWNPFVEPGSL-------RPLGPALLPLTVSTVG 332

BLAST of Spg033793 vs. TAIR 10
Match: AT4G40080.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 8.3e-45
Identity = 118/325 (36.31%), Postives = 178/325 (54.77%), Query Frame = 0

Query: 20  NLIHALKDQASIIKATF---SIHRRSSSIKVAVVRATTHDPGNPPSDGRVAAVLALGNDF 79
           +LI  +KD+AS  KA     +   ++ S  ++V+RATTHDP  PP +  +A +L+ G   
Sbjct: 9   DLIGRIKDKASQSKAALVSSNTKSKTLSFHLSVLRATTHDPSTPPGNRHLAVILSAGTGS 68

Query: 80  RSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFYGGRNFLN 139
           R +TA + +E++M+RLHTT  A VA+KSL  +H IV  G F L+DQ++  P  GGRN+L 
Sbjct: 69  R-ATASSAVESIMERLHTTGDACVALKSLIIIHHIVKHGRFILQDQLSVFPASGGRNYLK 128

Query: 140 LSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLRSRNCEIEDKEGRKV--- 199
           LSAFRD     M +LSSWVRWYA  +EH +   R +    ++ S +  I  +E  ++   
Sbjct: 129 LSAFRDEKSPLMWELSSWVRWYALYLEHLLSTSRIMG--FFISSTSSTIHKEEYEEMVSS 188

Query: 200 ----DLSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVKEI 259
               DL  E+D LVG +E  C++P+        L  ++ +LV E+Y     E++ R  E 
Sbjct: 189 LTNSDLLREIDALVGLLEEACKIPDLPFSGGKSLADKITQLVGEDYVSSINELYTRFNEF 248

Query: 260 GDRVESLSLDELTQLVGILTRFENCRRKLSVLF---VNRGKNEDLWELVKNTKGKL--VE 319
            +R  +LS  +  +LV  L R E+C+ +LS +      RG  +  W LV   KG +  +E
Sbjct: 249 KERSNTLSFGDTIELVCALKRLESCKERLSEICHGNWKRGWIDGFWGLVLEVKGIIGNLE 308

Query: 320 QKERKEEKRMIVVEMRADSVESTRF 330
               + EK ++    R    ES RF
Sbjct: 309 DNYGQIEKSIVGFGKRDKGYESARF 330

BLAST of Spg033793 vs. TAIR 10
Match: AT5G10410.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 105.5 bits (262), Expect = 8.9e-23
Identity = 97/313 (30.99%), Postives = 157/313 (50.16%), Query Frame = 0

Query: 20  NLIHALKDQASIIKATFSIHRRSSSIK---VAVVRATTHDPGNPPSDGRVAAVLALGNDF 79
           ++I   KD+ASI KA       S+++K   +A++++TT  P  PP+   V+AV++  N  
Sbjct: 7   HIIGKFKDKASIGKARLVHSFGSTAVKYIHLALLKSTTRTPNKPPNSDYVSAVISYSNSR 66

Query: 80  RSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFYGGRNFLN 139
            +  AF+   A + RL  T +A VA KSL  +H ++     + RD+  F     GRN L 
Sbjct: 67  YAPAAFS---AALWRLRVTKNAIVATKSLIVIHKLI----KSSRDK--FEGLGHGRNNLK 126

Query: 140 LSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGI-LYLRSRNCEIEDKEGRKVDL 199
           L+ F D S +   +LS W+RWY   ++    V + L      L +   ++E+K+      
Sbjct: 127 LNEFSDKSSNLTLELSQWIRWYGQYLDRLSWVPKVLGSFPNLLVNPKDKVEEKDRVSSYQ 186

Query: 200 SG----ELDVLVGFVERICEVPESLHLQRNDLVYEVVRLVMENYRLVQREIWVRVKEIGD 259
           +G    + D LV F E IC  PE   + +N +V E+  LV+E+Y  + R + VR++ + +
Sbjct: 187 TGYIIRQTDSLVSFFEHICTRPEIPPMFQNKIVDEIRELVIEDYFKIVRLVMVRLQVLFE 246

Query: 260 R--------VESLSLDELTQLVGILTRFENCRRKLSVLFVN-RGKNEDLWELVKNTKGKL 316
           R        +  L L++ + L   L R   C+  LS LF   R   +D W LV+  K   
Sbjct: 247 RLIKPGVKPIGDLGLNDFSLL---LVRLVECKESLSGLFWRCRRLADDFWCLVEMLKA-- 303

BLAST of Spg033793 vs. TAIR 10
Match: AT5G65370.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 84.7 bits (208), Expect = 1.6e-16
Identity = 91/310 (29.35%), Postives = 144/310 (46.45%), Query Frame = 0

Query: 17  KLKNLIHALKDQASIIKATFSIHRRSS----SIKVAVVRATTHDPGNPPSDGRVAAVLAL 76
           KL  L   LKD+AS +K    +H  SS    +I +A+++AT+H   NPPSD  V      
Sbjct: 3   KLATLNGILKDEASQMKLNV-VHLCSSVNAKTIDLALLKATSHTSNNPPSDKYVT----- 62

Query: 77  GNDFRSSTAFAC-----IEALMQRLHTTSSAAVAMKSLFTLHIIV-----IRGPFNLRDQ 136
              F  ST   C     ++A++ RL  T+   VA K L  LH +V       G  +LR+ 
Sbjct: 63  ---FLQSTIDTCYGPDTVDAILHRLRVTTDVCVAAKCLILLHKMVKSESGYNGEDSLRNN 122

Query: 137 V---AFCPFYGGRNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNVIVQRKLDGILYLR 196
           +         GG N L L+     S     +L+ WV+WY   ++  + +   L     ++
Sbjct: 123 INHRTLIYTQGGSN-LKLNDLNVNSSRFTRELTPWVQWYKQYLDCYLSIAEVLGITPNIK 182

Query: 197 SRNCEIEDK--EGRKVD------LSGELDVLVGFVERICEVPESLHLQRNDLVYEVVRLV 256
            +N   EDK  E ++V       +  ++D LV   E I + P++   + N +V E+  L+
Sbjct: 183 EKN---EDKRLETQRVSSYPMDCILKQIDFLVELFEHISDRPKAPQSKLNKIVIEMTELM 242

Query: 257 MENYRLVQREIWVRVKEIGDRVESLSLDELTQLVGILTRFENCRRKLSVLFVNRGKN--E 300
           +++Y    R + +R +E+  RV      +  +LV +L + ENC+  LS  F  R K    
Sbjct: 243 VQDYFSAIRLMRIRFEELNVRVA-----KPNELVPVLEKLENCKEGLSE-FSWRSKYLIA 293

BLAST of Spg033793 vs. TAIR 10
Match: AT4G02650.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 1.9e-12
Identity = 54/155 (34.84%), Postives = 86/155 (55.48%), Query Frame = 0

Query: 15  NKKLKNLIHALKDQASIIKATFSIHRRSSS---IKVAVVRATTHDPGNPPSDGRVAAVLA 74
           + KLK  I A+KDQ S+  A   +  RSSS   +++AVV+AT HD   P  D  +  +L 
Sbjct: 3   SSKLKRAIGAVKDQTSVGLA--KVGGRSSSLTELEIAVVKATRHD-DYPAEDKYIREILC 62

Query: 75  LGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFYGG 134
           L +  R+  + AC+  L +RL+ T + +VA+K+L  +  ++  G      ++ F    G 
Sbjct: 63  LTSYSRNYVS-ACVATLSRRLNKTKNWSVALKTLILIQRLLTDGDRAYEQEIFFATRRGT 122

Query: 135 RNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNV 167
           R  LN+S FRD S S+  D S++VR YA  ++  +
Sbjct: 123 R-LLNMSDFRDASQSDSWDYSAFVRTYALYLDERL 152

BLAST of Spg033793 vs. TAIR 10
Match: AT1G03050.1 (ENTH/ANTH/VHS superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 1.3e-10
Identity = 51/155 (32.90%), Postives = 82/155 (52.90%), Query Frame = 0

Query: 15  NKKLKNLIHALKDQASIIKATFSIHRRSSSIK---VAVVRATTHDPGNPPSDGRVAAVLA 74
           + K K  I A+KDQ S+  A   ++ RS+S+    VA+V+AT H+   P  +  +  +L+
Sbjct: 3   SSKFKRAIGAVKDQTSVGLA--KVNGRSASLSELDVAIVKATRHEE-FPAEEKYIREILS 62

Query: 75  LGNDFRSSTAFACIEALMQRLHTTSSAAVAMKSLFTLHIIVIRGPFNLRDQVAFCPFYGG 134
           L   +  S   AC+  L +RL+ T    VA+K+L  +  ++  G      ++ F    G 
Sbjct: 63  L-TSYSRSYINACVSTLSRRLNKTKCWTVALKTLILIQRLLGEGDQAYEQEIFFATRRGT 122

Query: 135 RNFLNLSAFRDVSDSEMSDLSSWVRWYAAVVEHNV 167
           R  LN+S FRDVS S   D S++VR YA  ++  +
Sbjct: 123 R-LLNMSDFRDVSRSNSWDYSAFVRTYALYLDERL 152

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038884022.11.4e-16385.59putative clathrin assembly protein At4g40080 [Benincasa hispida][more]
XP_022131457.12.1e-16283.66putative clathrin assembly protein At4g40080 [Momordica charantia][more]
XP_008445571.14.4e-15783.66PREDICTED: putative clathrin assembly protein At4g40080 [Cucumis melo] >KAA00651... [more]
XP_004152749.13.6e-15181.97putative clathrin assembly protein At4g40080 [Cucumis sativus] >KGN62720.1 hypot... [more]
KAG6598708.13.4e-13374.29putative clathrin assembly protein, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
Q8L9361.2e-4336.31Putative clathrin assembly protein At4g40080 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8H0W91.3e-2130.99Putative clathrin assembly protein At5g10410 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9FKQ22.3e-1529.35Putative clathrin assembly protein At5g65370 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8GX472.6e-1134.84Putative clathrin assembly protein At4g02650 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SA651.9e-0932.90Putative clathrin assembly protein At1g03050 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1BR259.9e-16383.66putative clathrin assembly protein At4g40080 OS=Momordica charantia OX=3673 GN=L... [more]
A0A5A7VCW12.1e-15783.66Putative clathrin assembly protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3BDW72.1e-15783.66putative clathrin assembly protein At4g40080 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0LLA11.8e-15181.97ENTH domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369220 PE=4 S... [more]
A0A6J1K8Z02.2e-13374.86putative clathrin assembly protein At4g40080 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G40080.18.3e-4536.31ENTH/ANTH/VHS superfamily protein [more]
AT5G10410.18.9e-2330.99ENTH/ANTH/VHS superfamily protein [more]
AT5G65370.11.6e-1629.35ENTH/ANTH/VHS superfamily protein [more]
AT4G02650.11.9e-1234.84ENTH/ANTH/VHS superfamily protein [more]
AT1G03050.11.3e-1032.90ENTH/ANTH/VHS superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013809ENTH domainSMARTSM00273enth_2coord: 43..176
e-value: 0.0011
score: 19.3
IPR013809ENTH domainPROSITEPS50942ENTHcoord: 37..176
score: 12.054218
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 16..173
e-value: 1.3E-26
score: 95.1
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 43..172
IPR011417AP180 N-terminal homology (ANTH) domainPFAMPF07651ANTHcoord: 45..174
e-value: 3.3E-12
score: 46.0
NoneNo IPR availablePANTHERPTHR22951:SF24CLATHRIN ASSEMBLY PROTEINcoord: 13..361
NoneNo IPR availablePANTHERPTHR22951CLATHRIN ASSEMBLY PROTEINcoord: 13..361
NoneNo IPR availableCDDcd16987ANTH_N_AP180_plantcoord: 45..168
e-value: 2.51465E-48
score: 157.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg033793.1Spg033793.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0072583 clathrin-dependent endocytosis
biological_process GO:0006900 vesicle budding from membrane
cellular_component GO:0005905 clathrin-coated pit
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0005794 Golgi apparatus
molecular_function GO:0005545 1-phosphatidylinositol binding
molecular_function GO:0032050 clathrin heavy chain binding
molecular_function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
molecular_function GO:0000149 SNARE binding
molecular_function GO:0005543 phospholipid binding