Spg033765 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg033765 vs. NCBI nr
Match: XP_038885396.1 (protein ESSENTIAL FOR POTEXVIRUS ACCUMULATION 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 3152.8 bits (8173), Expect = 0.0e+00
Identity = 1646/1827 (90.09%), Postives = 1720/1827 (94.14%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHGIGTGENHF+ QPAYGN MD MKGSENYED+N+IQ
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDTMKGSENYEDINDIQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKEVFRPSLTD+EIGRRDRWHDEERENNSSMRKDRWRDGEKE GDSRKMDRW EDSS R
Sbjct: 84   KKKEVFRPSLTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDSRKMDRWNEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSDS+NRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRLDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
            LDKN SHVSNYGKNDRDGDHYRPWRS++SQ RGKG+P HHQ+QTPIKQV AFSHRGR DN
Sbjct: 204  LDKNLSHVSNYGKNDRDGDHYRPWRSNSSQVRGKGDPPHHQSQTPIKQVPAFSHRGRGDN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
            TPPT+S+GRGIISSGVNPTNSIYSSSN LGASSEKSGREPYHYKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSIYSSSNYLGASSEKSGREPYHYKYSRTKLLDVFRTTNLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
            SQ   KDGFVPVPTLTLD+PLEPLALCAPT EEMTFLKGIDKGEIVSSGAPQVSKDGRNS
Sbjct: 324  SQQTPKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGF-DDYNDDKDDSTTKPGHTNYSEVSTERQVPYHR 435
            SEF QTRRTKL VSPS GSREDL HGF DDYNDDKDD TTKPGHTNYSEVSTERQVPYHR
Sbjct: 384  SEFMQTRRTKLSVSPSLGSREDLPHGFVDDYNDDKDDGTTKPGHTNYSEVSTERQVPYHR 443

Query: 436  PQSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDAS 495
            PQSKIEAIQEHM H SS FKSEAFRED+NAMRK DEVPVNRESSVKGG+ VHPGSTWDAS
Sbjct: 444  PQSKIEAIQEHMTHTSSTFKSEAFREDNNAMRKTDEVPVNRESSVKGGTNVHPGSTWDAS 503

Query: 496  SLEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGE 555
            SLEQP NTS+PD R+NPNNISSGTPDKGWLQ SKNLNDGWGSNTATPSYPKDNPKWQTGE
Sbjct: 504  SLEQPLNTSLPDRRENPNNISSGTPDKGWLQSSKNLNDGWGSNTATPSYPKDNPKWQTGE 563

Query: 556  ESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 615
            ESIIRRQLSGILDKEQLARKT QSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL
Sbjct: 564  ESIIRRQLSGILDKEQLARKTVQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 623

Query: 616  DLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHT 675
            DLPVRP N PNDLPF+ALGDVMPHLRSKAKPPPGFSGPK NEFAD++GNASYGSLGKLHT
Sbjct: 624  DLPVRPVNAPNDLPFAALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKLHT 683

Query: 676  GLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNL 735
            GLNEIDT+RNE RHKHGSTVEAENRFLESLMSGN+GSSPLEK+AFSEGVPGYFGNN ++L
Sbjct: 684  GLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKNAFSEGVPGYFGNNSSSL 743

Query: 736  SSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLS 795
            SSLGIDNGNNLFLLAKRMELERQRSL+NPYAFWPGID TSKVSKPDIGLDDPIQQAKLLS
Sbjct: 744  SSLGIDNGNNLFLLAKRMELERQRSLSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKLLS 803

Query: 796  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDL 855
            SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFA QCA DPLQSKLDLHHDL
Sbjct: 804  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFA-QCAPDPLQSKLDLHHDL 863

Query: 856  NLSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 915
            NLSSQAPFGFQQQRL PQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL
Sbjct: 864  NLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 923

Query: 916  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 975
            QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHF DPSFG
Sbjct: 924  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFIDPSFG 983

Query: 976  QLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNVN 1035
            QLQGAP+PIGNAS DP+QVQ SREKFQIGSQ PLNV+TDRA  FGNMALQVTQG SYNVN
Sbjct: 984  QLQGAPMPIGNASTDPAQVQQSREKFQIGSQKPLNVVTDRAAPFGNMALQVTQGASYNVN 1043

Query: 1036 SEDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNED 1095
             EDP + LPHQMFGNVVQQKSW  AL EQLND  P D+L GSKV EGS+ PGLT++SNED
Sbjct: 1044 LEDPPLALPHQMFGNVVQQKSWTPALSEQLNDTCPNDMLPGSKVAEGSLFPGLTAQSNED 1103

Query: 1096 VNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEPLPLKTDEVSVAIPPSEVRN 1155
            VNLVP SSDSHT KALEQI+EDVPRLDAT TS A DA+VEPLPLKT E+SVAI P+EV +
Sbjct: 1104 VNLVPKSSDSHTSKALEQIAEDVPRLDATVTSLASDALVEPLPLKTAEISVAIQPAEVHD 1163

Query: 1156 ---SIPVSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEVQEPKKSSDKKTKKQKS 1215
               SIP SVP +KV+EASMPVEKLERDVCKD+TSLETELKNVEVQEP+KSSDKKTKKQKS
Sbjct: 1164 FEISIPDSVPALKVQEASMPVEKLERDVCKDETSLETELKNVEVQEPRKSSDKKTKKQKS 1223

Query: 1216 SKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSSDTSSSPQKTRDGDAKI 1275
            SKLLSSDQ KDSK+SAIQQ KQSKSGK ENDLKLKADNIVGKSSDTSSSP+K RDGD K 
Sbjct: 1224 SKLLSSDQAKDSKSSAIQQLKQSKSGKSENDLKLKADNIVGKSSDTSSSPRKIRDGDGKT 1283

Query: 1276 AIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWKVASSFKPKS 1335
            ++VDSQPVQSSAS+IN+WND ET+Q+K+D+RL+GSDSV NSQ QSGQRAWKVASSFKPKS
Sbjct: 1284 SVVDSQPVQSSASAINTWNDGETIQMKDDARLVGSDSVLNSQTQSGQRAWKVASSFKPKS 1343

Query: 1336 LLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHKDSVNSEPSE 1395
            LLEIQEEEQK AHTETAVS+ISTSINS+SLSTPWAGIVS+ DPK SREIHKDS  SE SE
Sbjct: 1344 LLEIQEEEQKRAHTETAVSDISTSINSMSLSTPWAGIVSSSDPKTSREIHKDSGISESSE 1403

Query: 1396 KHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSDSAQIASSPQVMAAQAEPMDDNFIE 1455
            KHE+LL SRSRKSQLHDLLAEDDMEKS AGDVRVSDS QIASSP+VMA QAEP+DDNFIE
Sbjct: 1404 KHENLLTSRSRKSQLHDLLAEDDMEKSTAGDVRVSDSVQIASSPRVMATQAEPLDDNFIE 1463

Query: 1456 AKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKDAMPAIPSGP 1515
            AKDTKKSRKKSAKAK VGTKAT +VPSADV V SSPIEKGKISRQTQQEK+AMPAIPSGP
Sbjct: 1464 AKDTKKSRKKSAKAKGVGTKATPAVPSADV-VASSPIEKGKISRQTQQEKEAMPAIPSGP 1523

Query: 1516 SFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQERKISSAQHSHQIPTPQKA 1575
            SFGDFVLWKGE ANVAP PAWSSDSGKVPKPTSLRDIQKEQ RKIS+AQHSHQ PTPQKA
Sbjct: 1524 SFGDFVLWKGETANVAPPPAWSSDSGKVPKPTSLRDIQKEQGRKISAAQHSHQNPTPQKA 1583

Query: 1576 QPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGPIESKQENQQV 1635
            QPTQ GRSSSTSTPSWALSASSPSKAASSPLQN+P+QSK+GGDDDLFWGPIE+KQENQQV
Sbjct: 1584 QPTQAGRSSSTSTPSWALSASSPSKAASSPLQNIPTQSKHGGDDDLFWGPIEAKQENQQV 1643

Query: 1636 DVRLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDSVTKHSE 1695
            DVRLGSHGSWGNR+  AKA ASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQD VTKHSE
Sbjct: 1644 DVRLGSHGSWGNRSASAKAVASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDPVTKHSE 1703

Query: 1696 AMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQFLNFK 1755
            AMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAEL LIENLGSYDPDH+FIDQFLN+K
Sbjct: 1704 AMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELFLIENLGSYDPDHDFIDQFLNYK 1763

Query: 1756 ELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAGGDLDPDVP-GRDGSAKGGGKKKGKKG 1815
            ELLPADVLEIAFQSRNDRKVSAIA+REVNSGNAGGD+DPDVP GRDGSAK GGKKKGKKG
Sbjct: 1764 ELLPADVLEIAFQSRNDRKVSAIASREVNSGNAGGDVDPDVPLGRDGSAKSGGKKKGKKG 1823

Query: 1816 KKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            KKVSPSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 KKVSPSVLGFNVVSNRIMMGEIQTVED 1848

BLAST of Spg033765 vs. NCBI nr
Match: XP_038885397.1 (protein ESSENTIAL FOR POTEXVIRUS ACCUMULATION 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 3140.9 bits (8142), Expect = 0.0e+00
Identity = 1643/1827 (89.93%), Postives = 1717/1827 (93.98%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHGIGTGENHF+ QPAYGN MD MKGSENYED+N+IQ
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDTMKGSENYEDINDIQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKEVFRPSLTD+EIGRRDRWHDEERENNSSMRKDRWRDGEKE GDSRKMDRW EDSS R
Sbjct: 84   KKKEVFRPSLTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDSRKMDRWNEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSDS+NRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRLDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
            LDKN SHVSNYGKNDRDGDHYRPWRS++SQ RGKG+P HHQ+QTPIKQV AFSHRGR DN
Sbjct: 204  LDKNLSHVSNYGKNDRDGDHYRPWRSNSSQVRGKGDPPHHQSQTPIKQVPAFSHRGRGDN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
            TPPT+S+GRGIISSGVNPTNSIYSSSN LGASSEKSGREPYHYKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSIYSSSNYLGASSEKSGREPYHYKYSRTKLLDVFRTTNLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
            SQ   KDGFVPVPTLTLD+PLEPLALCAPT EEMTFLKGIDKGEIVSSGAPQVSKDGRNS
Sbjct: 324  SQQTPKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGF-DDYNDDKDDSTTKPGHTNYSEVSTERQVPYHR 435
            SEF QTRRTKL VSPS GSREDL HGF DDYNDDKDD TTKPGHTNYSEVSTERQVPYHR
Sbjct: 384  SEFMQTRRTKLSVSPSLGSREDLPHGFVDDYNDDKDDGTTKPGHTNYSEVSTERQVPYHR 443

Query: 436  PQSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDAS 495
            PQSKIEAIQEHM H SS FKSEAFRED+NAMRK DEVPVNRESSVKGG+ VHPGSTWDAS
Sbjct: 444  PQSKIEAIQEHMTHTSSTFKSEAFREDNNAMRKTDEVPVNRESSVKGGTNVHPGSTWDAS 503

Query: 496  SLEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGE 555
            SLEQP NTS+PD R+NPNNISSGTPDKGWLQ SKNLNDGWGSNTATPSYPKDNPKWQTGE
Sbjct: 504  SLEQPLNTSLPDRRENPNNISSGTPDKGWLQSSKNLNDGWGSNTATPSYPKDNPKWQTGE 563

Query: 556  ESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 615
            ESIIRRQLSGILDKEQLARKT QSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL
Sbjct: 564  ESIIRRQLSGILDKEQLARKTVQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 623

Query: 616  DLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHT 675
            DLPVRP N PNDLPF+ALGDVMPHLRSKAKPPPGFSGPK NEFAD++GNASYGSLGKLHT
Sbjct: 624  DLPVRPVNAPNDLPFAALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKLHT 683

Query: 676  GLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNL 735
            GLNEIDT+RNE RHKHGSTVEAENRFLESLMSGN+GSSPLEK+AFSE   GYFGNN ++L
Sbjct: 684  GLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKNAFSE---GYFGNNSSSL 743

Query: 736  SSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLS 795
            SSLGIDNGNNLFLLAKRMELERQRSL+NPYAFWPGID TSKVSKPDIGLDDPIQQAKLLS
Sbjct: 744  SSLGIDNGNNLFLLAKRMELERQRSLSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKLLS 803

Query: 796  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDL 855
            SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFA QCA DPLQSKLDLHHDL
Sbjct: 804  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFA-QCAPDPLQSKLDLHHDL 863

Query: 856  NLSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 915
            NLSSQAPFGFQQQRL PQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL
Sbjct: 864  NLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 923

Query: 916  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 975
            QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHF DPSFG
Sbjct: 924  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFIDPSFG 983

Query: 976  QLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNVN 1035
            QLQGAP+PIGNAS DP+QVQ SREKFQIGSQ PLNV+TDRA  FGNMALQVTQG SYNVN
Sbjct: 984  QLQGAPMPIGNASTDPAQVQQSREKFQIGSQKPLNVVTDRAAPFGNMALQVTQGASYNVN 1043

Query: 1036 SEDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNED 1095
             EDP + LPHQMFGNVVQQKSW  AL EQLND  P D+L GSKV EGS+ PGLT++SNED
Sbjct: 1044 LEDPPLALPHQMFGNVVQQKSWTPALSEQLNDTCPNDMLPGSKVAEGSLFPGLTAQSNED 1103

Query: 1096 VNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEPLPLKTDEVSVAIPPSEVRN 1155
            VNLVP SSDSHT KALEQI+EDVPRLDAT TS A DA+VEPLPLKT E+SVAI P+EV +
Sbjct: 1104 VNLVPKSSDSHTSKALEQIAEDVPRLDATVTSLASDALVEPLPLKTAEISVAIQPAEVHD 1163

Query: 1156 ---SIPVSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEVQEPKKSSDKKTKKQKS 1215
               SIP SVP +KV+EASMPVEKLERDVCKD+TSLETELKNVEVQEP+KSSDKKTKKQKS
Sbjct: 1164 FEISIPDSVPALKVQEASMPVEKLERDVCKDETSLETELKNVEVQEPRKSSDKKTKKQKS 1223

Query: 1216 SKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSSDTSSSPQKTRDGDAKI 1275
            SKLLSSDQ KDSK+SAIQQ KQSKSGK ENDLKLKADNIVGKSSDTSSSP+K RDGD K 
Sbjct: 1224 SKLLSSDQAKDSKSSAIQQLKQSKSGKSENDLKLKADNIVGKSSDTSSSPRKIRDGDGKT 1283

Query: 1276 AIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWKVASSFKPKS 1335
            ++VDSQPVQSSAS+IN+WND ET+Q+K+D+RL+GSDSV NSQ QSGQRAWKVASSFKPKS
Sbjct: 1284 SVVDSQPVQSSASAINTWNDGETIQMKDDARLVGSDSVLNSQTQSGQRAWKVASSFKPKS 1343

Query: 1336 LLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHKDSVNSEPSE 1395
            LLEIQEEEQK AHTETAVS+ISTSINS+SLSTPWAGIVS+ DPK SREIHKDS  SE SE
Sbjct: 1344 LLEIQEEEQKRAHTETAVSDISTSINSMSLSTPWAGIVSSSDPKTSREIHKDSGISESSE 1403

Query: 1396 KHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSDSAQIASSPQVMAAQAEPMDDNFIE 1455
            KHE+LL SRSRKSQLHDLLAEDDMEKS AGDVRVSDS QIASSP+VMA QAEP+DDNFIE
Sbjct: 1404 KHENLLTSRSRKSQLHDLLAEDDMEKSTAGDVRVSDSVQIASSPRVMATQAEPLDDNFIE 1463

Query: 1456 AKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKDAMPAIPSGP 1515
            AKDTKKSRKKSAKAK VGTKAT +VPSADV V SSPIEKGKISRQTQQEK+AMPAIPSGP
Sbjct: 1464 AKDTKKSRKKSAKAKGVGTKATPAVPSADV-VASSPIEKGKISRQTQQEKEAMPAIPSGP 1523

Query: 1516 SFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQERKISSAQHSHQIPTPQKA 1575
            SFGDFVLWKGE ANVAP PAWSSDSGKVPKPTSLRDIQKEQ RKIS+AQHSHQ PTPQKA
Sbjct: 1524 SFGDFVLWKGETANVAPPPAWSSDSGKVPKPTSLRDIQKEQGRKISAAQHSHQNPTPQKA 1583

Query: 1576 QPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGPIESKQENQQV 1635
            QPTQ GRSSSTSTPSWALSASSPSKAASSPLQN+P+QSK+GGDDDLFWGPIE+KQENQQV
Sbjct: 1584 QPTQAGRSSSTSTPSWALSASSPSKAASSPLQNIPTQSKHGGDDDLFWGPIEAKQENQQV 1643

Query: 1636 DVRLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDSVTKHSE 1695
            DVRLGSHGSWGNR+  AKA ASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQD VTKHSE
Sbjct: 1644 DVRLGSHGSWGNRSASAKAVASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDPVTKHSE 1703

Query: 1696 AMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQFLNFK 1755
            AMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAEL LIENLGSYDPDH+FIDQFLN+K
Sbjct: 1704 AMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELFLIENLGSYDPDHDFIDQFLNYK 1763

Query: 1756 ELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAGGDLDPDVP-GRDGSAKGGGKKKGKKG 1815
            ELLPADVLEIAFQSRNDRKVSAIA+REVNSGNAGGD+DPDVP GRDGSAK GGKKKGKKG
Sbjct: 1764 ELLPADVLEIAFQSRNDRKVSAIASREVNSGNAGGDVDPDVPLGRDGSAKSGGKKKGKKG 1823

Query: 1816 KKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            KKVSPSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 KKVSPSVLGFNVVSNRIMMGEIQTVED 1845

BLAST of Spg033765 vs. NCBI nr
Match: XP_022144321.1 (uncharacterized protein LOC111014032 [Momordica charantia])

HSP 1 Score: 3118.2 bits (8083), Expect = 0.0e+00
Identity = 1637/1830 (89.45%), Postives = 1705/1830 (93.17%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHGIGTGENHF+  PA+ N MDMMK SENYED N++Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASENYEDTNDMQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKEVFRPSL DAEIGRRDRWHDEERENNSSMRKDRWRDGEKE GDSRKMDRW EDSS R
Sbjct: 84   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSD SNRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
             DKN SHVSNYGKNDRDGDHYRPWRSSASQGRGKGEP HHQTQTP+KQV AFSHRGRADN
Sbjct: 204  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
             PPT+S+GRGIISSGV+ TN+IYSSSNSLGASSEKSGR+PYHYKYSRTKLLDVYRTTNLT
Sbjct: 264  IPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
             +P +KDGFVPVP+LTL++PLEPLALCAP+ EEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTERQV-PYHR 435
            SEF QTRR KLGVSPSPGSREDL HGFDDYNDDKDDSTTKPGH NYSE+STERQ+ PYHR
Sbjct: 384  SEFMQTRRAKLGVSPSPGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYHR 443

Query: 436  PQSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDAS 495
            PQSKIE IQEHMAHAS KFKSEAFREDDNA+RKADEVPVNRESSVKGG+TVHPGS WDAS
Sbjct: 444  PQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDAS 503

Query: 496  SLEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGE 555
            SLEQP NTS+PDWRDN NNI SGTPDKGWLQP KNLNDGWGSNTATPSYPKDNPKWQTGE
Sbjct: 504  SLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQTGE 563

Query: 556  ESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 615
            ESIIRRQLSGILDKEQLARKT QSAPED+QLHYIDPSGAIQGPFSGADIIQWFEGGYFGL
Sbjct: 564  ESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 623

Query: 616  DLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHT 675
            DLPVRP + PNDLPFSALGDVMPHLRSKAKPPPGFSGPK NEF D +GN SYGSLGKLHT
Sbjct: 624  DLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKLHT 683

Query: 676  GLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNL 735
            GLNEIDTLRNE  HKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE   GYFGNNPNNL
Sbjct: 684  GLNEIDTLRNETTHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE---GYFGNNPNNL 743

Query: 736  SSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLS 795
             SLGIDNGNNLFLLAKRMELERQRSL NPYAFWPGID T+KVSKPD+GLDDPIQQAKLLS
Sbjct: 744  PSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLLS 803

Query: 796  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDL 855
            SI+DHSRQTSHSQ ADMSAILQGLSDKAPPGINDVA WSKFASQCASDPLQSKLDLHHDL
Sbjct: 804  SIMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHDL 863

Query: 856  NLSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 915
            NLSSQAPFGFQQQRL PQPSLTNLLAQATDNPTLTPDKFL SSLSQDPQLISKLQQQHLL
Sbjct: 864  NLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHLL 923

Query: 916  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 975
            QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG
Sbjct: 924  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 983

Query: 976  QLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNVN 1035
            QLQGAPIPIGNAS DPSQVQLSREKFQIGSQ PLNV    ATTFGNM LQVTQG SYNVN
Sbjct: 984  QLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNV----ATTFGNMPLQVTQGASYNVN 1043

Query: 1036 SEDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNED 1095
             EDPS+ LPHQMFGNV+QQKSWNAALPEQ ND HPKD+L GSKVGEG  LPG+ SKS+ED
Sbjct: 1044 PEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSSED 1103

Query: 1096 VNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEPLPLKTDEVSVAIPPSEVR- 1155
            VNLVP SSD++T K LE+ SEDVP LDAT TS A DA +EPLPLKT EVSVAIPP EVR 
Sbjct: 1104 VNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEVRI 1163

Query: 1156 --NSIPVSVPVVKVEEASMPVEKLERD-VCKDDTSLETELKNVEVQEPKKSSDKKTKKQK 1215
               SIP SVPV+KV+EASMPVEKL RD VCKD+T LETELKNVEVQEP+KSSDKKTKKQK
Sbjct: 1164 SEISIPESVPVLKVQEASMPVEKLGRDVVCKDET-LETELKNVEVQEPRKSSDKKTKKQK 1223

Query: 1216 SSKLLSSDQVKDSKNSAIQQSKQSKSGKPE-NDLKLKADNIVGKSSDTSSSPQKTRDGDA 1275
            SSKLLSSDQ KDSKNSAIQQSKQSKSGKPE NDLKLKAD++VGKSSDTSSSP+K RD DA
Sbjct: 1224 SSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDADA 1283

Query: 1276 KIAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWKVASSFKP 1335
            KIA+VDSQP QSSAS++NSWND ETVQVK+DSRL GSDSVPNSQ QSGQRAWK A+SF+ 
Sbjct: 1284 KIAVVDSQPFQSSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSFRA 1343

Query: 1336 KSLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHKDSVNSEP 1395
            KSLLEIQEEEQKMAHTE AVSEIS S+NS SLSTPWAGIVSNLDPKASREIHKD VNSEP
Sbjct: 1344 KSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLDPKASREIHKDFVNSEP 1403

Query: 1396 SEKHESLLNSRSRKSQLHDLLAEDDMEKS-GAGDVRVSDSAQIASSPQVMAAQAEPMDDN 1455
            SEKHESLLNSRSRKSQLHDLLAEDDMEKS G GDVRVSDS QIASSPQVM  QAE  DDN
Sbjct: 1404 SEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTDDN 1463

Query: 1456 FIEAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKDAMPAIP 1515
            FIEAK+TKKSRKKSAKAK VGTKA++S PSADVPV SSP+EKGKISRQTQQEKDA PAIP
Sbjct: 1464 FIEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPAIP 1523

Query: 1516 SGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQERKISSAQHSHQIPTP 1575
            SGPSFGDFVLWKGEAANVAPAPAWSSDSGKV KPTSLRDIQKEQ RK+SSAQHSHQIPTP
Sbjct: 1524 SGPSFGDFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIPTP 1583

Query: 1576 QKAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGPIESKQEN 1635
            QKAQPTQVGRSSSTSTPSWALSASSPSKAA SPLQN+PSQSKYGGDDDLFWGPIESKQEN
Sbjct: 1584 QKAQPTQVGRSSSTSTPSWALSASSPSKAA-SPLQNIPSQSKYGGDDDLFWGPIESKQEN 1643

Query: 1636 QQVDVRLGSHGSWGNRNTPAK-AAASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDSVT 1695
            QQVDVR GSH SWGNRNTP+K AAASTGLLSRQKSSGGKADY SSSPAQS+QKGKQD +T
Sbjct: 1644 QQVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDPIT 1703

Query: 1696 KHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQF 1755
            KHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGS+DPDHEFIDQF
Sbjct: 1704 KHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFIDQF 1763

Query: 1756 LNFKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAGGDLDPDVPGRDGSAKGGGKKKG 1815
            LN+KELLPADVL+IAFQ RNDRKVS IA R++NSG+AGGDLDPDVP   GSAKGGGKKKG
Sbjct: 1764 LNYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVP--VGSAKGGGKKKG 1823

Query: 1816 KKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            KKGKKVSPSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 KKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1842

BLAST of Spg033765 vs. NCBI nr
Match: XP_023546983.1 (uncharacterized protein LOC111805921 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3048.1 bits (7901), Expect = 0.0e+00
Identity = 1600/1832 (87.34%), Postives = 1697/1832 (92.63%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHG+GTGENHF+ QP YGN MD MKGSEN EDMNEIQ
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGMGTGENHFSHQPVYGNRMDTMKGSENSEDMNEIQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKE+FRPSLTD+EIGRRDRWHDEEREN+SS+RKDRWRDGEKE GD RKMDRW EDSS R
Sbjct: 84   KKKEIFRPSLTDSEIGRRDRWHDEERENSSSVRKDRWRDGEKEIGDGRKMDRWNEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
            LDKNFSHVSNYGKNDRDGDHYRPWRSS+SQGRGKGEP HHQTQTPIKQV AFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSSQGRGKGEPPHHQTQTPIKQVPAFSHRGRADN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
            TPPT+S+GRGIISSGVNP NSIYSS +SLGASSEKSGREPY+YKYSRTKLLDV+RTT+LT
Sbjct: 264  TPPTFSLGRGIISSGVNPPNSIYSSPHSLGASSEKSGREPYYYKYSRTKLLDVFRTTSLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
            SQ  +KDGFVPVPTLTLD+PLEPLALC PT EEMTFLKGIDKGEIVSSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCVPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTERQVPYHRP 435
            SEF QTRRTKLGVSPS GSREDL HGFDD NDDKDDSTTKPGHTNYSEV+TERQ+PYHRP
Sbjct: 384  SEFMQTRRTKLGVSPSLGSREDLPHGFDDCNDDKDDSTTKPGHTNYSEVTTERQLPYHRP 443

Query: 436  QSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDASS 495
                      +AHASS FKSEAFREDDNAMRKADE PV+RESSVKGG+ V  G TWDASS
Sbjct: 444  ----------LAHASSTFKSEAFREDDNAMRKADEGPVSRESSVKGGTNVQHGRTWDASS 503

Query: 496  LEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGEE 555
            LEQ  NTS+PDWRDNPNNI+SGTPDKG LQ SKNLNDGWGSN+ATPSYPK+NPKW TG+E
Sbjct: 504  LEQLLNTSLPDWRDNPNNINSGTPDKG-LQSSKNLNDGWGSNSATPSYPKENPKWHTGDE 563

Query: 556  SIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGLD 615
            SI+RRQLSGILDKEQLARKT QSAPEDLQ HYIDPSGAIQGPFSGADIIQWFEGGYFGLD
Sbjct: 564  SILRRQLSGILDKEQLARKTVQSAPEDLQFHYIDPSGAIQGPFSGADIIQWFEGGYFGLD 623

Query: 616  LPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHTG 675
            LPVR AN PNDLPFSALGD MPHLRSKAKPPPGF GPK NEFADT+GNAS GSLGKLHTG
Sbjct: 624  LPVRLANAPNDLPFSALGDAMPHLRSKAKPPPGFCGPKQNEFADTLGNASIGSLGKLHTG 683

Query: 676  LNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNLS 735
            LNEID LRNE RHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSEG+PGYFGNN NNL 
Sbjct: 684  LNEIDPLRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGLPGYFGNNSNNLP 743

Query: 736  SLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLSS 795
            SLGIDNGNNLFLLAKRMELERQRSL+NPYAFWPGID +SKVSKPDIGLDDPIQQAKLLSS
Sbjct: 744  SLGIDNGNNLFLLAKRMELERQRSLSNPYAFWPGIDASSKVSKPDIGLDDPIQQAKLLSS 803

Query: 796  IIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDLN 855
            IIDHSRQTSH Q ADMSAILQGLSDKAPPGINDVAGWSKFASQCA DPLQSKLDLHHDLN
Sbjct: 804  IIDHSRQTSHPQSADMSAILQGLSDKAPPGINDVAGWSKFASQCAPDPLQSKLDLHHDLN 863

Query: 856  LSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLLQ 915
            LSSQAPFGFQQQRL PQP LTNLLAQATDN TLTPDKFLPSSLSQDPQLISKLQQQHLLQ
Sbjct: 864  LSSQAPFGFQQQRLQPQPPLTNLLAQATDNSTLTPDKFLPSSLSQDPQLISKLQQQHLLQ 923

Query: 916  LHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLL-QQQQLLSQVLSEHQSRQHFGDPSFG 975
            LHSQVPFSAQQMSLLDK+LLLKQQQKQEEQQQLL QQQQLLSQVLS+HQSRQHF DPSFG
Sbjct: 924  LHSQVPFSAQQMSLLDKILLLKQQQKQEEQQQLLQQQQQLLSQVLSDHQSRQHFVDPSFG 983

Query: 976  QLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNVN 1035
            QL GAPIPIGNAS DPSQVQLSREKFQIGSQ PLNVLTD ATT+GN+ALQ TQG SYNVN
Sbjct: 984  QLHGAPIPIGNASNDPSQVQLSREKFQIGSQKPLNVLTDHATTYGNIALQATQGASYNVN 1043

Query: 1036 SEDPSVVLPHQMFGNVV-QQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNE 1095
            SEDPS++LP QMFGNVV QQKSW  A+PEQLND  PKD++ GS V EGS+LPG++SKSNE
Sbjct: 1044 SEDPSLILPPQMFGNVVQQQKSWTTAIPEQLNDTRPKDVIPGSNVVEGSLLPGMSSKSNE 1103

Query: 1096 DVNLVPTSSDSHT-IKALEQISEDVPRLDATDTSFAP-DAIVEPLPLKTDEVSVAIPPSE 1155
            DVNLVP SSDSHT IK  E+ISEDVPRLDAT TSFA  DA VEPLPLK  E+SVAIPP  
Sbjct: 1104 DVNLVPKSSDSHTIIKTSEKISEDVPRLDATVTSFASVDATVEPLPLKNAEISVAIPPPA 1163

Query: 1156 VRN---SIPVSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEVQEPKKSSDKKTKK 1215
            V N   S+P SVP VKV+EASMP+EKL RD  +D+TSLE E+KNVEVQEP+KSSDKKTKK
Sbjct: 1164 VHNIEISVPDSVPAVKVQEASMPMEKLARDASRDETSLEAEVKNVEVQEPRKSSDKKTKK 1223

Query: 1216 QKSSKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSSDTSSSPQKTRDGD 1275
            QKSSKLLSSDQ KDSKNS IQQSKQSKSGK ENDLKLK+DNIVGKSSDT+ SP+K RDGD
Sbjct: 1224 QKSSKLLSSDQAKDSKNSGIQQSKQSKSGKSENDLKLKSDNIVGKSSDTACSPRKIRDGD 1283

Query: 1276 AKIAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWKVASSFK 1335
             KIAIVDSQ  QS AS++NSWND ETVQV+++SRLIGSDSV NSQ QS QRAWK+AS+FK
Sbjct: 1284 GKIAIVDSQLDQSCASAVNSWNDGETVQVRDESRLIGSDSVLNSQTQSSQRAWKIASNFK 1343

Query: 1336 PKSLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHKDSVNSE 1395
            PKSLLEIQEEEQKMAHTET VS+ISTSIN++SLSTPWAGIVS+ DPKASREIHKDSVNSE
Sbjct: 1344 PKSLLEIQEEEQKMAHTETVVSDISTSINTMSLSTPWAGIVSSSDPKASREIHKDSVNSE 1403

Query: 1396 PSEKHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSDSAQIASSPQVMAAQAEPMDDN 1455
             SEKHE+LL SRSRKSQLHDLLAEDDMEKSGAGDVRVSD+ QIASSPQVMA +AEPMD+N
Sbjct: 1404 SSEKHENLLTSRSRKSQLHDLLAEDDMEKSGAGDVRVSDTVQIASSPQVMAVRAEPMDEN 1463

Query: 1456 FIEAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKDAMPAIP 1515
            FIEAKDTKKSRKKSAKAK +GTKA+S+VPSADVPVG SP+EKGKISRQTQQEK+AMPAIP
Sbjct: 1464 FIEAKDTKKSRKKSAKAKGIGTKASSAVPSADVPVGLSPVEKGKISRQTQQEKEAMPAIP 1523

Query: 1516 SGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQERKISSAQHSHQIPTP 1575
            SGPSFGDFVLWKGE ANVAPAPAWSSDSGKV KPTSLRDIQ EQERKIS+AQHS QI TP
Sbjct: 1524 SGPSFGDFVLWKGEVANVAPAPAWSSDSGKVAKPTSLRDIQMEQERKISAAQHSQQISTP 1583

Query: 1576 QKAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGPIESKQEN 1635
            QKAQPTQVGRSS T+TPSW+LSASSPSKAASSPLQN+P+QSK+GGDDDLFWGPIESKQEN
Sbjct: 1584 QKAQPTQVGRSSRTTTPSWSLSASSPSKAASSPLQNIPTQSKHGGDDDLFWGPIESKQEN 1643

Query: 1636 QQVDVRLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDSVTK 1695
            Q+VDVR GSHG+WGNRNTP KA ASTGLLSRQKSSGGKAD+LSSSPAQSSQKGKQD +TK
Sbjct: 1644 QRVDVRPGSHGNWGNRNTPVKAVASTGLLSRQKSSGGKADHLSSSPAQSSQKGKQDPITK 1703

Query: 1696 HSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQFL 1755
            HSEAMGFRDWCE+ECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQFL
Sbjct: 1704 HSEAMGFRDWCENECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQFL 1763

Query: 1756 NFKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNA-GGDLDPDV-PGRDGSAK-GGGKK 1815
            N+KELLPADV+EIAF+SRN+RKVSA+A+R+VNSGNA GGDLDPDV  GRDGSAK GGGKK
Sbjct: 1764 NYKELLPADVIEIAFRSRNERKVSAMASRDVNSGNAGGGDLDPDVSAGRDGSAKSGGGKK 1823

Query: 1816 KGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            KGKKGKK++PSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 KGKKGKKINPSVLGFNVVSNRIMMGEIQTVED 1844

BLAST of Spg033765 vs. NCBI nr
Match: KAA0065243.1 (uncharacterized protein E6C27_scaffold82G005840 [Cucumis melo var. makuwa])

HSP 1 Score: 3043.8 bits (7890), Expect = 0.0e+00
Identity = 1603/1837 (87.26%), Postives = 1695/1837 (92.27%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHGIGTGENHF+ QPAYGN MDMMKGSENYEDMN+ Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDMMKGSENYEDMNDTQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKEVFRPS+TD+EIGRRDRWHDEERENNSSMRKDRWRDGEKE GD RKMDRW EDSS R
Sbjct: 84   KKKEVFRPSVTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDGRKMDRWNEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSDS+NRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
            LDKNFSHVSNYGKNDRDGDHYRPWRSS++QGRGKGEP HHQTQTP KQV AFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSAQGRGKGEPPHHQTQTPSKQVPAFSHRGRADN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
            TPPT+S+GRGIISSGVNPTNS+YSS N LGASSEKSGREP +YKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSVYSSPNYLGASSEKSGREPCYYKYSRTKLLDVFRTTNLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
            SQ  +KDGFVPVPTLTLD+PLEPLALCAPT EEMTFLKGIDKGEIVSSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTERQVPYHRP 435
            SEF Q RRTKLGVSPS GSREDL HGFDDYNDDKDDSTTK GHTNYSEVSTERQVPYHRP
Sbjct: 384  SEFMQARRTKLGVSPSLGSREDLPHGFDDYNDDKDDSTTKLGHTNYSEVSTERQVPYHRP 443

Query: 436  QSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDASS 495
            QSK EAIQE M H S  FKSEAFREDDNA+RK DEVP NRESSVKG + +H  STWDASS
Sbjct: 444  QSKNEAIQEQMGHTSGNFKSEAFREDDNALRKTDEVPGNRESSVKGATNIHSSSTWDASS 503

Query: 496  LEQPRNTSMPDWRDNPNN-ISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGE 555
            LEQ  NTS+PDWRDNPNN ISSGTPDKGW+Q SKNL+DGWGSNT TPSY KDN KWQ+ E
Sbjct: 504  LEQSLNTSLPDWRDNPNNIISSGTPDKGWVQSSKNLSDGWGSNTTTPSYAKDNSKWQSTE 563

Query: 556  ESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 615
            ESIIRRQLSGILDKEQL+RKT Q APED+QLHYIDPSGAIQGPF GADIIQWFEGGYFGL
Sbjct: 564  ESIIRRQLSGILDKEQLSRKTVQPAPEDMQLHYIDPSGAIQGPFGGADIIQWFEGGYFGL 623

Query: 616  DLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHT 675
            DLPVRP N P+DLPFSALGDVMPHLRSKAKPPPGFSGPK NEFAD++GNASYGSLGKLHT
Sbjct: 624  DLPVRPTNAPSDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKLHT 683

Query: 676  GLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNL 735
            GLNEIDT+RNE RHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSEGVPGYFG NPN+L
Sbjct: 684  GLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGVPGYFGTNPNSL 743

Query: 736  SSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLS 795
            SSLG+DNGNNLFLLAKRMELERQRS++NPYAFWPGID TSKVSKPDIGLDDPIQQAKLLS
Sbjct: 744  SSLGMDNGNNLFLLAKRMELERQRSMSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKLLS 803

Query: 796  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDL 855
            SIIDHSRQTSHSQ  DMSAILQGLSDKAPPGIN+VAGWSKFA   A DPLQSKLDLHH+L
Sbjct: 804  SIIDHSRQTSHSQSPDMSAILQGLSDKAPPGINEVAGWSKFAH--APDPLQSKLDLHHEL 863

Query: 856  NLSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 915
            NLSSQAPFGFQQQRL PQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL
Sbjct: 864  NLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 923

Query: 916  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 975
            QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQH  DPSFG
Sbjct: 924  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHLIDPSFG 983

Query: 976  QLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNVN 1035
            QLQGAPIPIGNAS DPSQVQ  REKFQIGSQ PLNV+TDRA  FGNMALQVTQG SYNVN
Sbjct: 984  QLQGAPIPIGNASTDPSQVQQPREKFQIGSQKPLNVVTDRAIPFGNMALQVTQGASYNVN 1043

Query: 1036 SEDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNED 1095
             EDPS+ LPHQMFGN VQQK W   LPEQL D  PKD+L GS VGE S+ PGLTSK  ED
Sbjct: 1044 PEDPSLALPHQMFGN-VQQKGWTPGLPEQLTDTRPKDMLPGSIVGEASLFPGLTSKQIED 1103

Query: 1096 VNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEPLPLKTDEVSVAIPPSEVRN 1155
            V+ V  SSDSHT++ALEQI E VPRLD T TS A DA+VEPLPLKT ++SVA+ P+EV +
Sbjct: 1104 VSHVQKSSDSHTVQALEQIGEAVPRLDETATSLASDAMVEPLPLKTADISVALQPAEVDD 1163

Query: 1156 ---SIP--------VSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEVQEPKKSSD 1215
               SIP         SVPV+KV+EAS+PV+KLERD CKDDTSLETELKNVEVQEPKKSSD
Sbjct: 1164 TEVSIPDSCPTQTADSVPVLKVQEASVPVQKLERDGCKDDTSLETELKNVEVQEPKKSSD 1223

Query: 1216 KKTKKQKSSKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSSDTSSSPQK 1275
            KKTKKQKSSK LSSDQ KDSKNSAIQQSKQSKSGK ENDLKLKADNI+GK+SD +SSP+K
Sbjct: 1224 KKTKKQKSSKSLSSDQAKDSKNSAIQQSKQSKSGKSENDLKLKADNIMGKASDMASSPRK 1283

Query: 1276 TRDG-DAKIAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWK 1335
             RDG D KI+IVD+QPVQSSAS++N+W+D +TVQVK+D++L+GSDSV NSQ QS QRAWK
Sbjct: 1284 IRDGDDGKISIVDNQPVQSSASAMNTWSDGDTVQVKDDAKLVGSDSVLNSQTQSSQRAWK 1343

Query: 1336 VASSFKPKSLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHK 1395
            VASSFKPKSLLEIQEEEQK AHTETAVSEISTSI S+SLSTPWAGIVS+ DPKAS+EIHK
Sbjct: 1344 VASSFKPKSLLEIQEEEQKRAHTETAVSEISTSITSMSLSTPWAGIVSSSDPKASKEIHK 1403

Query: 1396 DSVNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSDSAQIASSPQVMAAQA 1455
            DSV SE SEKHE+LL SRSRKSQLHDLLAED+MEKSGA DVRVSDS QIASSP+V+A QA
Sbjct: 1404 DSVISESSEKHENLLTSRSRKSQLHDLLAEDNMEKSGASDVRVSDSVQIASSPRVVATQA 1463

Query: 1456 EPMDDNFIEAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKD 1515
            EPMDDNFIEAKDTKKSRKKSAKAK VGTK ++ VPSADVPV SSPIEKGKISRQTQQEK+
Sbjct: 1464 EPMDDNFIEAKDTKKSRKKSAKAKGVGTKPSAPVPSADVPVASSPIEKGKISRQTQQEKE 1523

Query: 1516 AMPAIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQERKIS-SAQH 1575
            AMP IPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQ RK S +AQH
Sbjct: 1524 AMPVIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQGRKTSAAAQH 1583

Query: 1576 SHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGP 1635
            SHQIPTPQKAQP+QVGRSSSTSTPSWALSASSPSKAASSPLQNVP+QS +GGDDDLFWGP
Sbjct: 1584 SHQIPTPQKAQPSQVGRSSSTSTPSWALSASSPSKAASSPLQNVPTQSNHGGDDDLFWGP 1643

Query: 1636 IESKQENQQVDVRLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKADYLSSSPAQSSQKG 1695
            IESK+ENQQVDVRLGS+ +WGNRNTPAK AASTG+LSRQKSSGGKADYLSSSPAQSSQKG
Sbjct: 1644 IESKKENQQVDVRLGSN-NWGNRNTPAK-AASTGVLSRQKSSGGKADYLSSSPAQSSQKG 1703

Query: 1696 KQDSVTKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDH 1755
            KQD VTKHSEAMGFRDWCESECVRLIGTKDTSFLE+CLKQSRSEAELLLI+NLGSYDPDH
Sbjct: 1704 KQDPVTKHSEAMGFRDWCESECVRLIGTKDTSFLEYCLKQSRSEAELLLIQNLGSYDPDH 1763

Query: 1756 EFIDQFLNFKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAGGDLDPDVP-GRDGSAK 1815
            +FIDQFLN+KELL ADVLEIAFQSRNDRKVSAIA+REVNSGNAGGDLDPDVP GRDGSAK
Sbjct: 1764 DFIDQFLNYKELLAADVLEIAFQSRNDRKVSAIASREVNSGNAGGDLDPDVPVGRDGSAK 1823

Query: 1816 GGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
             GGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQT+ED
Sbjct: 1824 SGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTIED 1855

BLAST of Spg033765 vs. ExPASy Swiss-Prot
Match: Q9FMM3 (Protein ESSENTIAL FOR POTEXVIRUS ACCUMULATION 1 OS=Arabidopsis thaliana OX=3702 GN=EXA1 PE=1 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 816/1855 (43.99%), Postives = 1117/1855 (60.22%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            ++QGS+N IPLSPQWLL KPGE+K G+GTG+ +      YGNH D+++ + N E+  +  
Sbjct: 28   DIQGSDNAIPLSPQWLLSKPGENKTGMGTGDPN-----QYGNHSDVVRTTGNGEETLDNL 87

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKK+VFRPSL DAE GRRDRW DEER+  SS+R DRWR+G+K++GD++K+DRW  D+ A 
Sbjct: 88   KKKDVFRPSLLDAESGRRDRWRDEERDTLSSVRNDRWRNGDKDSGDNKKVDRW--DNVAP 147

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
             F E RRGP++RW+DS N+D    +QRRESKWN+RWGPDDKE E  R K  + G+DG++ 
Sbjct: 148  KFGEQRRGPNDRWTDSGNKDAAP-EQRRESKWNSRWGPDDKEAEIPRNKWDEPGKDGEII 207

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSH-RGRAD 255
             +K  S  ++      DGDHYRPWR   SQGRG+GE  H+Q+ TP KQV +FSH RGR +
Sbjct: 208  REKGPSLPTS------DGDHYRPWR--PSQGRGRGEALHNQS-TPNKQVTSFSHSRGRGE 267

Query: 256  NTPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEK--SG-REPYHYKYSRTKLLDVYRT 315
            NT   +S GRG +S G +   S  + S+  G++S+K  SG  EP H +YSR KLLDVYR 
Sbjct: 268  NT-AIFSAGRGRMSPGGSIFTSAPNQSHPPGSASDKGESGPGEPPHLRYSRMKLLDVYRM 327

Query: 316  TNLTSQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKD 375
             +        DGF+ VP+LT ++P +PLALCAP+++E+  L  I+KG+IVSSGAPQ SKD
Sbjct: 328  ADTECYEKFPDGFIEVPSLTSEEPTDPLALCAPSSDEVNVLDAIEKGKIVSSGAPQTSKD 387

Query: 376  ---GRNSSEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTER 435
               GRN  EF+Q RR +       GSRED++ G ++  D+  ++   P            
Sbjct: 388  GPTGRNPVEFSQPRRIR-----PAGSREDMTFGAEESKDESGETRNYP------------ 447

Query: 436  QVPYHRPQSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVN--RESSVKGGSTVH 495
                                   KF+ EA  E     R+ +E PV   +E S++G + V 
Sbjct: 448  ---------------------DDKFRPEASHEGYAPFRRGNEAPVRELKEPSMQGNAHVQ 507

Query: 496  PGSTWDASSLEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKD 555
              S W  SS  +  N +  DW D   +    + D  W  P  ++N   G+N   P   K 
Sbjct: 508  SASPWRQSSGGERSNRNSHDWNDPSADSRLKSSDSVWSHPKDSINHLGGNNMMLPQ-SKG 567

Query: 556  NPKWQTGEESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQW 615
              +WQ  E+  +RRQ S + D+EQ  RK   S+PE+L L+Y DP G IQGPFSG+DII W
Sbjct: 568  ESRWQISEDPSLRRQPSLVFDREQEVRKLLPSSPEELSLYYKDPQGLIQGPFSGSDIIGW 627

Query: 616  FEGGYFGLDLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASY 675
            FE GYFG+DL VR A+ PND PFS LGDVMPHLR+K+ PPPGF+G K NEF D  G +++
Sbjct: 628  FEAGYFGIDLLVRLASAPNDSPFSLLGDVMPHLRAKSGPPPGFTGAKQNEFVDAAGTSAF 687

Query: 676  GSLGKLHTGLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGY 735
              +GK+H+G+ E D L+N+ R+KH +   AENRF+ESLMSG L +S       ++GV GY
Sbjct: 688  PGVGKVHSGMGETDMLQNDMRYKHVAGTVAENRFIESLMSGGLTNS-------AQGVQGY 747

Query: 736  FGNNPNNLSSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDP 795
              N+   LS    D G +++LLAK++ELERQRS+ +PY++WPG +  + +   +      
Sbjct: 748  GVNSSGGLSLPVTDGGADMYLLAKKLELERQRSIPSPYSYWPGRESANLMPGSE------ 807

Query: 796  IQQAKLLSSIIDHSRQTSHSQGADMSAILQGLSDKAPPGIN-DVAGWSKFASQCASDPLQ 855
                    ++ ++++Q + S  +D+ +ILQG++D++ P ++  +  WS+        P+Q
Sbjct: 808  --------NVSENAQQPTRSPSSDLLSILQGVTDRSSPAVSGPLPAWSQ--------PIQ 867

Query: 856  SKLDLHHDLNLSSQAPFGFQQQRLPPQP-SLTNLLAQATDN---PTLTPDKFLPSSLSQD 915
             + DLHH     +Q PFG QQQRLP Q   L+ LL Q  +N     L+PD  L + LSQ+
Sbjct: 868  KESDLHHAKTFQTQIPFGVQQQRLPEQNLPLSGLLGQPMENNPGGMLSPDMMLAAGLSQE 927

Query: 916  PQLISKLQQQH-LLQLHSQVPFSAQ-QMSLLDKLLLLKQQQKQEEQQQLL-QQQQLLSQV 975
             Q ++ LQQQ  LLQL++Q P SAQ Q  L++K+LLLK Q KQEEQQQLL QQQQL SQV
Sbjct: 928  HQSLNLLQQQQLLLQLNAQTPLSAQHQRLLVEKMLLLKHQHKQEEQQQLLRQQQQLYSQV 987

Query: 976  LSEHQ-SRQHFGDPSFGQLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVL-TDRAT 1035
             ++ Q S+Q FGDPS+GQLQ        AS D  ++Q S++  Q+  Q  + V   +R  
Sbjct: 988  FADQQRSQQRFGDPSYGQLQ--------ASLDALRLQPSKDMSQVNQQVQVPVSHEERGI 1047

Query: 1036 TFGNMALQVTQGPSYNVNS-EDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSG 1095
               ++ L VT   +  V S E PS+ L +Q+FGNV  +      LP+Q++D H K+  S 
Sbjct: 1048 NLADL-LPVTHATNQTVASFETPSLHLQNQLFGNVDPR----MVLPDQIDDTHKKE--SK 1107

Query: 1096 SKVGEGSVLPGLTSKSNEDVNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEP 1155
            S+         + S  +E   L P    +H ++  E +S   P  +++  +     IVE 
Sbjct: 1108 SEYERTVSADYVNSLYSEKPVLSPGYHATHNVE--EPVS--YPNNESSTATMTAPEIVES 1167

Query: 1156 LPLKTDEVSVAIPPSEVRNSIPVSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEV 1215
              L+     +     EV   +    P                          TE+KN +V
Sbjct: 1168 KLLEEQSKDMYAGKGEVSIELSGETPA-------------------------TEVKNNDV 1227

Query: 1216 QEPKKSSDKKTKKQKSSKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSS 1275
               +K+S+KK++KQ++ +  ++D  K +  + +Q++K+ + G  ++        I GK  
Sbjct: 1228 SVARKTSEKKSRKQRAKQ--AADLAKSTSRAPLQETKKPQPGSADD------SEIKGK-- 1287

Query: 1276 DTSSSPQKTRDGDAKIAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQ 1335
             T  S     D D  + I  S    S+ S ++S  +V++V+ +E        S+ N++ Q
Sbjct: 1288 -TKKSADTLIDNDTHL-IKSSTATASNTSQMSS--EVDSVRGEE-------SSLQNTRTQ 1347

Query: 1336 SGQRAWKVASSFKPKSLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPK 1395
             G RAWK A  FKPKSLLEIQ EEQ++A  E    +IS+++NSV  + PWAGIV+N D  
Sbjct: 1348 PG-RAWKPAPGFKPKSLLEIQMEEQRVAQAEALAPKISSTVNSVGSAAPWAGIVTNSDSN 1407

Query: 1396 ASREIHKDSVNSEPS-EKHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSD-SAQIAS 1455
              RE H +S  ++    K ES+   +++KS LHDLLA+D   KS   +  V +  +   +
Sbjct: 1408 ILRETHGESAITQTGVVKPESVPTLKAKKSHLHDLLADDVFAKSSDKEREVMEIISNNDA 1467

Query: 1456 SPQVMAAQAEPM-DDNFIEAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGK 1515
              QV    AE   DDNFI+A++TKKSRKKSA+AK  G K  + VP+ D  + ++ +EKGK
Sbjct: 1468 FMQVTTTNAESFDDDNFIDARETKKSRKKSARAKTSGAKIAAHVPAVDTSLQTNSVEKGK 1527

Query: 1516 ISR-QTQQEKDAMPAIPSGPSFGDFVLWKGEAA-NVAPAPAWSSDSGKVPKPTSLRDIQK 1575
             SR   QQEK+ +PAIPSGPS GDFVLWKGE+  N  PA AWSS   K  KP+SLRDI K
Sbjct: 1528 SSRILQQQEKEVLPAIPSGPSLGDFVLWKGESVNNPPPAAAWSSGPKKSTKPSSLRDIVK 1587

Query: 1576 EQERKISSAQ-HSHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQS 1635
            EQE+  +S+      +PT QKA P Q  +  +    SW+ SASSPS+A S       SQS
Sbjct: 1588 EQEKMTTSSHPPPSPVPTTQKAIPPQAHQGGA----SWSRSASSPSQAVSQS----SSQS 1647

Query: 1636 KYGGDDDLFWGPIE-SKQENQQVDV-RLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKA 1695
            K  GDDDLFWGP+E S Q+ +Q D   L S  SWG +NTP K  A T L  ++  S G A
Sbjct: 1648 KSKGDDDLFWGPVEQSTQDTKQGDFPHLTSQNSWGTKNTPGKVNAGTSLNRQKSVSMGSA 1707

Query: 1696 DYLSSSP--AQSSQKGKQDSVTKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSE 1755
            D + SSP   Q+S KGK+++VTK +EA GFRDWC+SEC+RL+G++DTS LEFCLK SRSE
Sbjct: 1708 DRVLSSPVVTQASHKGKKEAVTKLTEANGFRDWCKSECLRLLGSEDTSVLEFCLKLSRSE 1714

Query: 1756 AELLLIENLGSYDPDHEFIDQFLNFKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAG 1815
            AE LLIENLGS DPDH+FID+FLN+K+LLP++V+EIAFQS    K S +  R     N G
Sbjct: 1768 AETLLIENLGSRDPDHKFIDKFLNYKDLLPSEVVEIAFQS----KGSGVGTR----NNTG 1714

Query: 1816 GDLDPD-VPGRDGSAKGGGKKKGKKGKKV--SPSVLGFNVVSNRIMMGEIQTVED 1838
             D   +     DG +K GGKKK KKGKKV  S SVLGFNVVSNRIMMGEIQT+ED
Sbjct: 1828 EDYYYNTTAANDGFSKVGGKKKAKKGKKVSLSASVLGFNVVSNRIMMGEIQTIED 1714

BLAST of Spg033765 vs. ExPASy Swiss-Prot
Match: Q6Y7W6 (GRB10-interacting GYF protein 2 OS=Homo sapiens OX=9606 GN=GIGYF2 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 7.6e-06
Identity = 38/130 (29.23%), Postives = 57/130 (43.85%), Query Frame = 0

Query: 533 GWGSNTATPSYPKDNPKWQTGEESIIRRQLSGILDKEQLARKTYQSAPEDLQL------- 592
           G GS +  P   +     +   E ++       LD E+LA K  +   + + +       
Sbjct: 474 GMGSVSTEPDDEEGLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAM 533

Query: 593 ---HYIDPSGAIQGPFSGADIIQWFEGGYFGLDLPVRPANPPNDLPFSALGDVM------ 647
              +Y DP G IQGPF+  ++ +WF+ GYF + L V+ A    D  F  LGD+M      
Sbjct: 534 QKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRA---CDESFQPLGDIMKMWGRV 593

BLAST of Spg033765 vs. ExPASy TrEMBL
Match: A0A6J1CT22 (uncharacterized protein LOC111014032 OS=Momordica charantia OX=3673 GN=LOC111014032 PE=4 SV=1)

HSP 1 Score: 3118.2 bits (8083), Expect = 0.0e+00
Identity = 1637/1830 (89.45%), Postives = 1705/1830 (93.17%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHGIGTGENHF+  PA+ N MDMMK SENYED N++Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSNPPAFRNRMDMMKASENYEDTNDMQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKEVFRPSL DAEIGRRDRWHDEERENNSSMRKDRWRDGEKE GDSRKMDRW EDSS R
Sbjct: 84   KKKEVFRPSLADAEIGRRDRWHDEERENNSSMRKDRWRDGEKELGDSRKMDRWVEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSD SNRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDLSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
             DKN SHVSNYGKNDRDGDHYRPWRSSASQGRGKGEP HHQTQTP+KQV AFSHRGRADN
Sbjct: 204  PDKNLSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPPHHQTQTPVKQVPAFSHRGRADN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
             PPT+S+GRGIISSGV+ TN+IYSSSNSLGASSEKSGR+PYHYKYSRTKLLDVYRTTNLT
Sbjct: 264  IPPTFSLGRGIISSGVSSTNNIYSSSNSLGASSEKSGRDPYHYKYSRTKLLDVYRTTNLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
             +P +KDGFVPVP+LTL++PLEPLALCAP+ EEMTFLKGIDKGEI+SSGAPQVSKDGRNS
Sbjct: 324  LEPSLKDGFVPVPSLTLEEPLEPLALCAPSTEEMTFLKGIDKGEIISSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTERQV-PYHR 435
            SEF QTRR KLGVSPSPGSREDL HGFDDYNDDKDDSTTKPGH NYSE+STERQ+ PYHR
Sbjct: 384  SEFMQTRRAKLGVSPSPGSREDLPHGFDDYNDDKDDSTTKPGHINYSELSTERQMPPYHR 443

Query: 436  PQSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDAS 495
            PQSKIE IQEHMAHAS KFKSEAFREDDNA+RKADEVPVNRESSVKGG+TVHPGS WDAS
Sbjct: 444  PQSKIETIQEHMAHASGKFKSEAFREDDNAIRKADEVPVNRESSVKGGATVHPGSAWDAS 503

Query: 496  SLEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGE 555
            SLEQP NTS+PDWRDN NNI SGTPDKGWLQP KNLNDGWGSNTATPSYPKDNPKWQTGE
Sbjct: 504  SLEQPLNTSVPDWRDNHNNIGSGTPDKGWLQPPKNLNDGWGSNTATPSYPKDNPKWQTGE 563

Query: 556  ESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 615
            ESIIRRQLSGILDKEQLARKT QSAPED+QLHYIDPSGAIQGPFSGADIIQWFEGGYFGL
Sbjct: 564  ESIIRRQLSGILDKEQLARKTIQSAPEDMQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 623

Query: 616  DLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHT 675
            DLPVRP + PNDLPFSALGDVMPHLRSKAKPPPGFSGPK NEF D +GN SYGSLGKLHT
Sbjct: 624  DLPVRPVSAPNDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFGDALGNTSYGSLGKLHT 683

Query: 676  GLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNL 735
            GLNEIDTLRNE  HKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE   GYFGNNPNNL
Sbjct: 684  GLNEIDTLRNETTHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSE---GYFGNNPNNL 743

Query: 736  SSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLS 795
             SLGIDNGNNLFLLAKRMELERQRSL NPYAFWPGID T+KVSKPD+GLDDPIQQAKLLS
Sbjct: 744  PSLGIDNGNNLFLLAKRMELERQRSLNNPYAFWPGIDATAKVSKPDVGLDDPIQQAKLLS 803

Query: 796  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDL 855
            SI+DHSRQTSHSQ ADMSAILQGLSDKAPPGINDVA WSKFASQCASDPLQSKLDLHHDL
Sbjct: 804  SIMDHSRQTSHSQSADMSAILQGLSDKAPPGINDVAAWSKFASQCASDPLQSKLDLHHDL 863

Query: 856  NLSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 915
            NLSSQAPFGFQQQRL PQPSLTNLLAQATDNPTLTPDKFL SSLSQDPQLISKLQQQHLL
Sbjct: 864  NLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLSSSLSQDPQLISKLQQQHLL 923

Query: 916  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 975
            QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG
Sbjct: 924  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 983

Query: 976  QLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNVN 1035
            QLQGAPIPIGNAS DPSQVQLSREKFQIGSQ PLNV    ATTFGNM LQVTQG SYNVN
Sbjct: 984  QLQGAPIPIGNASIDPSQVQLSREKFQIGSQKPLNV----ATTFGNMPLQVTQGASYNVN 1043

Query: 1036 SEDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNED 1095
             EDPS+ LPHQMFGNV+QQKSWNAALPEQ ND HPKD+L GSKVGEG  LPG+ SKS+ED
Sbjct: 1044 PEDPSLALPHQMFGNVIQQKSWNAALPEQFNDTHPKDMLPGSKVGEGPTLPGMISKSSED 1103

Query: 1096 VNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEPLPLKTDEVSVAIPPSEVR- 1155
            VNLVP SSD++T K LE+ SEDVP LDAT TS A DA +EPLPLKT EVSVAIPP EVR 
Sbjct: 1104 VNLVPHSSDNNTNKVLEKASEDVPSLDATVTSLASDATIEPLPLKTAEVSVAIPPVEVRI 1163

Query: 1156 --NSIPVSVPVVKVEEASMPVEKLERD-VCKDDTSLETELKNVEVQEPKKSSDKKTKKQK 1215
               SIP SVPV+KV+EASMPVEKL RD VCKD+T LETELKNVEVQEP+KSSDKKTKKQK
Sbjct: 1164 SEISIPESVPVLKVQEASMPVEKLGRDVVCKDET-LETELKNVEVQEPRKSSDKKTKKQK 1223

Query: 1216 SSKLLSSDQVKDSKNSAIQQSKQSKSGKPE-NDLKLKADNIVGKSSDTSSSPQKTRDGDA 1275
            SSKLLSSDQ KDSKNSAIQQSKQSKSGKPE NDLKLKAD++VGKSSDTSSSP+K RD DA
Sbjct: 1224 SSKLLSSDQAKDSKNSAIQQSKQSKSGKPELNDLKLKADSVVGKSSDTSSSPRKIRDADA 1283

Query: 1276 KIAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWKVASSFKP 1335
            KIA+VDSQP QSSAS++NSWND ETVQVK+DSRL GSDSVPNSQ QSGQRAWK A+SF+ 
Sbjct: 1284 KIAVVDSQPFQSSASAVNSWNDAETVQVKDDSRLTGSDSVPNSQSQSGQRAWKTATSFRA 1343

Query: 1336 KSLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHKDSVNSEP 1395
            KSLLEIQEEEQKMAHTE AVSEIS S+NS SLSTPWAGIVSNLDPKASREIHKD VNSEP
Sbjct: 1344 KSLLEIQEEEQKMAHTEPAVSEISISVNSTSLSTPWAGIVSNLDPKASREIHKDFVNSEP 1403

Query: 1396 SEKHESLLNSRSRKSQLHDLLAEDDMEKS-GAGDVRVSDSAQIASSPQVMAAQAEPMDDN 1455
            SEKHESLLNSRSRKSQLHDLLAEDDMEKS G GDVRVSDS QIASSPQVM  QAE  DDN
Sbjct: 1404 SEKHESLLNSRSRKSQLHDLLAEDDMEKSGGVGDVRVSDSVQIASSPQVMTTQAESTDDN 1463

Query: 1456 FIEAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKDAMPAIP 1515
            FIEAK+TKKSRKKSAKAK VGTKA++S PSADVPV SSP+EKGKISRQTQQEKDA PAIP
Sbjct: 1464 FIEAKETKKSRKKSAKAKGVGTKASASAPSADVPVASSPVEKGKISRQTQQEKDATPAIP 1523

Query: 1516 SGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQERKISSAQHSHQIPTP 1575
            SGPSFGDFVLWKGEAANVAPAPAWSSDSGKV KPTSLRDIQKEQ RK+SSAQHSHQIPTP
Sbjct: 1524 SGPSFGDFVLWKGEAANVAPAPAWSSDSGKVHKPTSLRDIQKEQGRKLSSAQHSHQIPTP 1583

Query: 1576 QKAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGPIESKQEN 1635
            QKAQPTQVGRSSSTSTPSWALSASSPSKAA SPLQN+PSQSKYGGDDDLFWGPIESKQEN
Sbjct: 1584 QKAQPTQVGRSSSTSTPSWALSASSPSKAA-SPLQNIPSQSKYGGDDDLFWGPIESKQEN 1643

Query: 1636 QQVDVRLGSHGSWGNRNTPAK-AAASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDSVT 1695
            QQVDVR GSH SWGNRNTP+K AAASTGLLSRQKSSGGKADY SSSPAQS+QKGKQD +T
Sbjct: 1644 QQVDVRQGSHSSWGNRNTPSKAAAASTGLLSRQKSSGGKADYPSSSPAQSTQKGKQDPIT 1703

Query: 1696 KHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQF 1755
            KHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGS+DPDHEFIDQF
Sbjct: 1704 KHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSFDPDHEFIDQF 1763

Query: 1756 LNFKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAGGDLDPDVPGRDGSAKGGGKKKG 1815
            LN+KELLPADVL+IAFQ RNDRKVS IA R++NSG+AGGDLDPDVP   GSAKGGGKKKG
Sbjct: 1764 LNYKELLPADVLDIAFQRRNDRKVSEIATRDMNSGSAGGDLDPDVP--VGSAKGGGKKKG 1823

Query: 1816 KKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            KKGKKVSPSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 KKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1842

BLAST of Spg033765 vs. ExPASy TrEMBL
Match: A0A5A7VAQ0 (GYF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005840 PE=4 SV=1)

HSP 1 Score: 3043.8 bits (7890), Expect = 0.0e+00
Identity = 1603/1837 (87.26%), Postives = 1695/1837 (92.27%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHGIGTGENHF+ QPAYGN MDMMKGSENYEDMN+ Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDMMKGSENYEDMNDTQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKEVFRPS+TD+EIGRRDRWHDEERENNSSMRKDRWRDGEKE GD RKMDRW EDSS R
Sbjct: 84   KKKEVFRPSVTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDGRKMDRWNEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSDS+NRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
            LDKNFSHVSNYGKNDRDGDHYRPWRSS++QGRGKGEP HHQTQTP KQV AFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSAQGRGKGEPPHHQTQTPSKQVPAFSHRGRADN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
            TPPT+S+GRGIISSGVNPTNS+YSS N LGASSEKSGREP +YKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSVYSSPNYLGASSEKSGREPCYYKYSRTKLLDVFRTTNLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
            SQ  +KDGFVPVPTLTLD+PLEPLALCAPT EEMTFLKGIDKGEIVSSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTERQVPYHRP 435
            SEF Q RRTKLGVSPS GSREDL HGFDDYNDDKDDSTTK GHTNYSEVSTERQVPYHRP
Sbjct: 384  SEFMQARRTKLGVSPSLGSREDLPHGFDDYNDDKDDSTTKLGHTNYSEVSTERQVPYHRP 443

Query: 436  QSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDASS 495
            QSK EAIQE M H S  FKSEAFREDDNA+RK DEVP NRESSVKG + +H  STWDASS
Sbjct: 444  QSKNEAIQEQMGHTSGNFKSEAFREDDNALRKTDEVPGNRESSVKGATNIHSSSTWDASS 503

Query: 496  LEQPRNTSMPDWRDNPNN-ISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGE 555
            LEQ  NTS+PDWRDNPNN ISSGTPDKGW+Q SKNL+DGWGSNT TPSY KDN KWQ+ E
Sbjct: 504  LEQSLNTSLPDWRDNPNNIISSGTPDKGWVQSSKNLSDGWGSNTTTPSYAKDNSKWQSTE 563

Query: 556  ESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 615
            ESIIRRQLSGILDKEQL+RKT Q APED+QLHYIDPSGAIQGPF GADIIQWFEGGYFGL
Sbjct: 564  ESIIRRQLSGILDKEQLSRKTVQPAPEDMQLHYIDPSGAIQGPFGGADIIQWFEGGYFGL 623

Query: 616  DLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHT 675
            DLPVRP N P+DLPFSALGDVMPHLRSKAKPPPGFSGPK NEFAD++GNASYGSLGKLHT
Sbjct: 624  DLPVRPTNAPSDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKLHT 683

Query: 676  GLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNL 735
            GLNEIDT+RNE RHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSEGVPGYFG NPN+L
Sbjct: 684  GLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGVPGYFGTNPNSL 743

Query: 736  SSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLS 795
            SSLG+DNGNNLFLLAKRMELERQRS++NPYAFWPGID TSKVSKPDIGLDDPIQQAKLLS
Sbjct: 744  SSLGMDNGNNLFLLAKRMELERQRSMSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKLLS 803

Query: 796  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDL 855
            SIIDHSRQTSHSQ  DMSAILQGLSDKAPPGIN+VAGWSKFA   A DPLQSKLDLHH+L
Sbjct: 804  SIIDHSRQTSHSQSPDMSAILQGLSDKAPPGINEVAGWSKFAH--APDPLQSKLDLHHEL 863

Query: 856  NLSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 915
            NLSSQAPFGFQQQRL PQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL
Sbjct: 864  NLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 923

Query: 916  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 975
            QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQH  DPSFG
Sbjct: 924  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHLIDPSFG 983

Query: 976  QLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNVN 1035
            QLQGAPIPIGNAS DPSQVQ  REKFQIGSQ PLNV+TDRA  FGNMALQVTQG SYNVN
Sbjct: 984  QLQGAPIPIGNASTDPSQVQQPREKFQIGSQKPLNVVTDRAIPFGNMALQVTQGASYNVN 1043

Query: 1036 SEDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNED 1095
             EDPS+ LPHQMFGN VQQK W   LPEQL D  PKD+L GS VGE S+ PGLTSK  ED
Sbjct: 1044 PEDPSLALPHQMFGN-VQQKGWTPGLPEQLTDTRPKDMLPGSIVGEASLFPGLTSKQIED 1103

Query: 1096 VNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEPLPLKTDEVSVAIPPSEVRN 1155
            V+ V  SSDSHT++ALEQI E VPRLD T TS A DA+VEPLPLKT ++SVA+ P+EV +
Sbjct: 1104 VSHVQKSSDSHTVQALEQIGEAVPRLDETATSLASDAMVEPLPLKTADISVALQPAEVDD 1163

Query: 1156 ---SIP--------VSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEVQEPKKSSD 1215
               SIP         SVPV+KV+EAS+PV+KLERD CKDDTSLETELKNVEVQEPKKSSD
Sbjct: 1164 TEVSIPDSCPTQTADSVPVLKVQEASVPVQKLERDGCKDDTSLETELKNVEVQEPKKSSD 1223

Query: 1216 KKTKKQKSSKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSSDTSSSPQK 1275
            KKTKKQKSSK LSSDQ KDSKNSAIQQSKQSKSGK ENDLKLKADNI+GK+SD +SSP+K
Sbjct: 1224 KKTKKQKSSKSLSSDQAKDSKNSAIQQSKQSKSGKSENDLKLKADNIMGKASDMASSPRK 1283

Query: 1276 TRDG-DAKIAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWK 1335
             RDG D KI+IVD+QPVQSSAS++N+W+D +TVQVK+D++L+GSDSV NSQ QS QRAWK
Sbjct: 1284 IRDGDDGKISIVDNQPVQSSASAMNTWSDGDTVQVKDDAKLVGSDSVLNSQTQSSQRAWK 1343

Query: 1336 VASSFKPKSLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHK 1395
            VASSFKPKSLLEIQEEEQK AHTETAVSEISTSI S+SLSTPWAGIVS+ DPKAS+EIHK
Sbjct: 1344 VASSFKPKSLLEIQEEEQKRAHTETAVSEISTSITSMSLSTPWAGIVSSSDPKASKEIHK 1403

Query: 1396 DSVNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSDSAQIASSPQVMAAQA 1455
            DSV SE SEKHE+LL SRSRKSQLHDLLAED+MEKSGA DVRVSDS QIASSP+V+A QA
Sbjct: 1404 DSVISESSEKHENLLTSRSRKSQLHDLLAEDNMEKSGASDVRVSDSVQIASSPRVVATQA 1463

Query: 1456 EPMDDNFIEAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKD 1515
            EPMDDNFIEAKDTKKSRKKSAKAK VGTK ++ VPSADVPV SSPIEKGKISRQTQQEK+
Sbjct: 1464 EPMDDNFIEAKDTKKSRKKSAKAKGVGTKPSAPVPSADVPVASSPIEKGKISRQTQQEKE 1523

Query: 1516 AMPAIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQERKIS-SAQH 1575
            AMP IPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQ RK S +AQH
Sbjct: 1524 AMPVIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQGRKTSAAAQH 1583

Query: 1576 SHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGP 1635
            SHQIPTPQKAQP+QVGRSSSTSTPSWALSASSPSKAASSPLQNVP+QS +GGDDDLFWGP
Sbjct: 1584 SHQIPTPQKAQPSQVGRSSSTSTPSWALSASSPSKAASSPLQNVPTQSNHGGDDDLFWGP 1643

Query: 1636 IESKQENQQVDVRLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKADYLSSSPAQSSQKG 1695
            IESK+ENQQVDVRLGS+ +WGNRNTPAK AASTG+LSRQKSSGGKADYLSSSPAQSSQKG
Sbjct: 1644 IESKKENQQVDVRLGSN-NWGNRNTPAK-AASTGVLSRQKSSGGKADYLSSSPAQSSQKG 1703

Query: 1696 KQDSVTKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDH 1755
            KQD VTKHSEAMGFRDWCESECVRLIGTKDTSFLE+CLKQSRSEAELLLI+NLGSYDPDH
Sbjct: 1704 KQDPVTKHSEAMGFRDWCESECVRLIGTKDTSFLEYCLKQSRSEAELLLIQNLGSYDPDH 1763

Query: 1756 EFIDQFLNFKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAGGDLDPDVP-GRDGSAK 1815
            +FIDQFLN+KELL ADVLEIAFQSRNDRKVSAIA+REVNSGNAGGDLDPDVP GRDGSAK
Sbjct: 1764 DFIDQFLNYKELLAADVLEIAFQSRNDRKVSAIASREVNSGNAGGDLDPDVPVGRDGSAK 1823

Query: 1816 GGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
             GGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQT+ED
Sbjct: 1824 SGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTIED 1855

BLAST of Spg033765 vs. ExPASy TrEMBL
Match: A0A1S3BBQ1 (uncharacterized protein LOC103487961 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487961 PE=4 SV=1)

HSP 1 Score: 3039.6 bits (7879), Expect = 0.0e+00
Identity = 1602/1837 (87.21%), Postives = 1694/1837 (92.22%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHGIGTGENHF+ QPAYGN MDMMKGSENYEDMN+ Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDMMKGSENYEDMNDTQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKEVFRPS+TD+EIGRRDRWHDEERENNSSMRKDRWRDGEKE GD RKMDRW EDSS R
Sbjct: 84   KKKEVFRPSVTDSEIGRRDRWHDEERENNSSMRKDRWRDGEKEMGDGRKMDRWNEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSDS+NRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
            LDKNFSHVSNYGKNDRDGDHYRPWRSS++QGRGKGEP HHQTQTP KQV AFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSAQGRGKGEPPHHQTQTPSKQVPAFSHRGRADN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
            TPPT+S+GRGIISSGVNPTNS+YSS N LGASSEKSGREP +YKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSVYSSPNYLGASSEKSGREPCYYKYSRTKLLDVFRTTNLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
            SQ  +KDGFVPVPTLTLD+PLEPLALCAPT EEMTFLKGIDKGEIVSSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTERQVPYHRP 435
            SEF Q RRTKLGVSPS GSREDL HGFDDYNDDKDDSTTK GHTNYSEVSTERQVPYHRP
Sbjct: 384  SEFMQARRTKLGVSPSLGSREDLPHGFDDYNDDKDDSTTKLGHTNYSEVSTERQVPYHRP 443

Query: 436  QSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDASS 495
            QSK EAIQE M H S  FKSEAFREDDNA+RK DEVP NRESSVKG + +H  STWDASS
Sbjct: 444  QSKNEAIQEQMGHTSGNFKSEAFREDDNALRKTDEVPGNRESSVKGATNIHSSSTWDASS 503

Query: 496  LEQPRNTSMPDWRDNPNN-ISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGE 555
            LEQ  NTS+PDWRDNPNN ISSGTPDKGW+Q SKNL+DGWGSNT TPSY KDN KWQ+ E
Sbjct: 504  LEQSLNTSLPDWRDNPNNIISSGTPDKGWVQSSKNLSDGWGSNTTTPSYAKDNSKWQSTE 563

Query: 556  ESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 615
            ESIIRRQLSGILDKEQL+RKT Q APED+QLHYIDPSGAIQGPF GADIIQWFEGGYFGL
Sbjct: 564  ESIIRRQLSGILDKEQLSRKTVQPAPEDMQLHYIDPSGAIQGPFGGADIIQWFEGGYFGL 623

Query: 616  DLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHT 675
            DLPVRP N P+DLPFSALGDVMPHLRSKAKPPPGFSGPK NEFAD++GNASYGSLGKLHT
Sbjct: 624  DLPVRPTNAPSDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNASYGSLGKLHT 683

Query: 676  GLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNL 735
            GLNEIDT+RNE RHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSEGVPGYFG NPN+L
Sbjct: 684  GLNEIDTMRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGVPGYFGTNPNSL 743

Query: 736  SSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLS 795
            SSLG+DNGNNLFLLAKRMELERQRS++NPYAFWPGID TSKVSKPDIGLDDPIQQAKLLS
Sbjct: 744  SSLGMDNGNNLFLLAKRMELERQRSMSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKLLS 803

Query: 796  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDL 855
            SIIDHSRQTSHSQ  DMSAILQGLSDKAPPGIN+VAGWSKFA   A DPLQSKLDLHH+L
Sbjct: 804  SIIDHSRQTSHSQSPDMSAILQGLSDKAPPGINEVAGWSKFAH--APDPLQSKLDLHHEL 863

Query: 856  NLSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 915
            NLSSQAPFGFQQQRL PQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL
Sbjct: 864  NLSSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 923

Query: 916  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 975
            QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQH  DPSFG
Sbjct: 924  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHLIDPSFG 983

Query: 976  QLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNVN 1035
            QLQGAPIPIGNAS DPSQVQ  REKFQIGSQ PLNV+TDRA  FGNMALQVTQG SYNVN
Sbjct: 984  QLQGAPIPIGNASTDPSQVQQPREKFQIGSQKPLNVVTDRAIPFGNMALQVTQGASYNVN 1043

Query: 1036 SEDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNED 1095
             EDPS+ LPHQMFGN VQQK W   LPEQL D  PKD+L GS VGE S+ PGLTSK  ED
Sbjct: 1044 PEDPSLALPHQMFGN-VQQKGWTPGLPEQLTDTRPKDMLPGSIVGEASLFPGLTSKQIED 1103

Query: 1096 VNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEPLPLKTDEVSVAIPPSEVRN 1155
            V+ V  SSDSHT++ALEQI E VPRLD T TS A DA+VEPLPLKT ++SVA+ P+EV +
Sbjct: 1104 VSHVQKSSDSHTVQALEQIGEAVPRLDETATSLASDAMVEPLPLKTADISVALQPAEVDD 1163

Query: 1156 ---SIP--------VSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEVQEPKKSSD 1215
               SIP         SVPV+KV+EAS+PV+KLERD  KDDTSLETELKNVEVQEPKKSSD
Sbjct: 1164 TEVSIPDSCSTQTADSVPVLKVQEASVPVQKLERDGYKDDTSLETELKNVEVQEPKKSSD 1223

Query: 1216 KKTKKQKSSKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSSDTSSSPQK 1275
            KKTKKQKSSK LSSDQ KDSKNSAIQQSKQSKSGK ENDLKLKADNI+GK+SD +SSP+K
Sbjct: 1224 KKTKKQKSSKSLSSDQAKDSKNSAIQQSKQSKSGKSENDLKLKADNIMGKASDMASSPRK 1283

Query: 1276 TRDG-DAKIAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWK 1335
             RDG D KI+IVD+QPVQSSAS++N+W+D +TVQVK+D++L+GSDSV NSQ QS QRAWK
Sbjct: 1284 IRDGDDGKISIVDNQPVQSSASAMNTWSDGDTVQVKDDAKLVGSDSVLNSQTQSSQRAWK 1343

Query: 1336 VASSFKPKSLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHK 1395
            VASSFKPKSLLEIQEEEQK AHTETAVSEISTSI S+SLSTPWAGIVS+ DPKAS+EIHK
Sbjct: 1344 VASSFKPKSLLEIQEEEQKRAHTETAVSEISTSITSMSLSTPWAGIVSSSDPKASKEIHK 1403

Query: 1396 DSVNSEPSEKHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSDSAQIASSPQVMAAQA 1455
            DSV SE SEKHE+LL SRSRKSQLHDLLAED+MEKSGA DVRVSDS QIASSP+V+A QA
Sbjct: 1404 DSVISESSEKHENLLTSRSRKSQLHDLLAEDNMEKSGASDVRVSDSVQIASSPRVVATQA 1463

Query: 1456 EPMDDNFIEAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKD 1515
            EPMDDNFIEAKDTKKSRKKSAKAK VGTK ++ VPSADVPV SSPIEKGKISRQTQQEK+
Sbjct: 1464 EPMDDNFIEAKDTKKSRKKSAKAKGVGTKPSAPVPSADVPVASSPIEKGKISRQTQQEKE 1523

Query: 1516 AMPAIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQERKIS-SAQH 1575
            AMP IPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQ RK S +AQH
Sbjct: 1524 AMPVIPSGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQGRKTSAAAQH 1583

Query: 1576 SHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGP 1635
            SHQIPTPQKAQP+QVGRSSSTSTPSWALSASSPSKAASSPLQNVP+QS +GGDDDLFWGP
Sbjct: 1584 SHQIPTPQKAQPSQVGRSSSTSTPSWALSASSPSKAASSPLQNVPTQSNHGGDDDLFWGP 1643

Query: 1636 IESKQENQQVDVRLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKADYLSSSPAQSSQKG 1695
            IESK+ENQQVDVRLGS+ +WGNRNTPAK AASTG+LSRQKSSGGKADYLSSSPAQSSQKG
Sbjct: 1644 IESKKENQQVDVRLGSN-NWGNRNTPAK-AASTGVLSRQKSSGGKADYLSSSPAQSSQKG 1703

Query: 1696 KQDSVTKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDH 1755
            KQD VTKHSEAMGFRDWCESECVRLIGTKDTSFLE+CLKQSRSEAELLLI+NLGSYDPDH
Sbjct: 1704 KQDPVTKHSEAMGFRDWCESECVRLIGTKDTSFLEYCLKQSRSEAELLLIQNLGSYDPDH 1763

Query: 1756 EFIDQFLNFKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAGGDLDPDVP-GRDGSAK 1815
            +FIDQFLN+KELL ADVLEIAFQSRNDRKVSAIA+REVNSGNAGGDLDPDVP GRDGSAK
Sbjct: 1764 DFIDQFLNYKELLAADVLEIAFQSRNDRKVSAIASREVNSGNAGGDLDPDVPVGRDGSAK 1823

Query: 1816 GGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
             GGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQT+ED
Sbjct: 1824 SGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTIED 1855

BLAST of Spg033765 vs. ExPASy TrEMBL
Match: A0A0A0LRG9 (GYF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361560 PE=4 SV=1)

HSP 1 Score: 3034.6 bits (7866), Expect = 0.0e+00
Identity = 1596/1829 (87.26%), Postives = 1686/1829 (92.18%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHGIGTGENHF+ QPAYGN MDMMKGSENYEDMN+ Q
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFSHQPAYGNRMDMMKGSENYEDMNDTQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKEVFRPSLTD+E GRRDRWHDEERENNSSMRKDRWRDGEKE GDSRKMDRW EDSS R
Sbjct: 84   KKKEVFRPSLTDSETGRRDRWHDEERENNSSMRKDRWRDGEKEMGDSRKMDRWNEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSDS+NRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSNNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
            LDKNFSHVSNYGKNDRDGDHYRPWRSS++QGRGKGE  HHQTQTP KQV AFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSAQGRGKGELPHHQTQTPSKQVPAFSHRGRADN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
            TPPT+S+GRGIISSGVNPTNSIYSS N LGASSEKSGREPY+YKYSRTKLLDV+RTTNLT
Sbjct: 264  TPPTFSLGRGIISSGVNPTNSIYSSPNYLGASSEKSGREPYYYKYSRTKLLDVFRTTNLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
            SQ  +KD FVPVPTLTLD+PLEPLALCAPT EEMTFLKGIDKGEIVSSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDVFVPVPTLTLDEPLEPLALCAPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTERQVPYHRP 435
            SEF Q RRTKLGVSPS GSREDL HGFDDYNDDKDDSTTK GHTNYSEVSTERQVPYHRP
Sbjct: 384  SEFMQARRTKLGVSPSLGSREDLPHGFDDYNDDKDDSTTKLGHTNYSEVSTERQVPYHRP 443

Query: 436  QSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDASS 495
            QSK EAIQE M H S  FKSEAFREDDNA+RK DEVP NRESSVKGG+ +HP STWDASS
Sbjct: 444  QSKNEAIQEQMGHTSGTFKSEAFREDDNALRKTDEVPGNRESSVKGGTNIHPSSTWDASS 503

Query: 496  LEQPRNTSMPDWRDNPNN-ISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGE 555
            LEQP NTS+PDWRDNPNN ISSGTPDKGW+Q SKNLNDGWGSN   PSY KDN KWQT E
Sbjct: 504  LEQPLNTSLPDWRDNPNNIISSGTPDKGWVQSSKNLNDGWGSNATNPSYAKDNSKWQTAE 563

Query: 556  ESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGL 615
            ESI+RRQLSGILDKEQL+RKT Q A EDLQLHYIDPSGAIQGPF GADIIQWFEGGYFGL
Sbjct: 564  ESILRRQLSGILDKEQLSRKTVQPAAEDLQLHYIDPSGAIQGPFGGADIIQWFEGGYFGL 623

Query: 616  DLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHT 675
            DLPVRP N P+DLPFSALGDVMPHLRSKAKPPPGFSGPK NEFAD++GN S+GSLGKLHT
Sbjct: 624  DLPVRPTNAPSDLPFSALGDVMPHLRSKAKPPPGFSGPKQNEFADSLGNPSFGSLGKLHT 683

Query: 676  GLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNL 735
            GLNEIDTLRNE RHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSEGVPGYFGNNPN+L
Sbjct: 684  GLNEIDTLRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGVPGYFGNNPNSL 743

Query: 736  SSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLS 795
            SSLGIDNGNNLFLLAKRMELERQRSL+NPYAFWPGID TSKVSKPDIGLDDPIQQAKLLS
Sbjct: 744  SSLGIDNGNNLFLLAKRMELERQRSLSNPYAFWPGIDATSKVSKPDIGLDDPIQQAKLLS 803

Query: 796  SIIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDL 855
            SIIDHSRQTSHSQ  DMSAILQGLSDKAPPGIN+VAGWSKF+   A DPLQSKLDLHHDL
Sbjct: 804  SIIDHSRQTSHSQSPDMSAILQGLSDKAPPGINEVAGWSKFSH--APDPLQSKLDLHHDL 863

Query: 856  NLSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 915
            NL SQAPFGFQQQRL PQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL
Sbjct: 864  NLPSQAPFGFQQQRLQPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLL 923

Query: 916  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHFGDPSFG 975
            QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQH  DPSFG
Sbjct: 924  QLHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLLQQQQLLSQVLSEHQSRQHLIDPSFG 983

Query: 976  QLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNVN 1035
            QLQGAPIPIGNASADPSQVQ  REKFQIGSQ PLNV+TDRA  FGNMALQVTQG SYNVN
Sbjct: 984  QLQGAPIPIGNASADPSQVQQPREKFQIGSQKPLNVVTDRAIPFGNMALQVTQGASYNVN 1043

Query: 1036 SEDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNED 1095
            SEDPS+ LPHQMFGN VQQK W   LPEQL D   KD+L GS VGE S+ PGLTSK +ED
Sbjct: 1044 SEDPSLALPHQMFGN-VQQKGWTPGLPEQLTDTRSKDMLPGSIVGEVSLFPGLTSKPSED 1103

Query: 1096 VNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEPLPLKTDEVSVAIPPSEVRN 1155
            V+ V  SSDSHTI+ALEQI EDVPRLDAT TS A D +VEPLPLKT ++SVA+ P+EV +
Sbjct: 1104 VSHVQKSSDSHTIQALEQIGEDVPRLDATATSLASDVMVEPLPLKTADISVALQPAEVHD 1163

Query: 1156 ---SIPVSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEVQEPKKSSDKKTKKQKS 1215
               SIP SVPV+KV+EASMPV+KLER  CKDDT+LETELKN+EVQEPKK SDKKTKKQKS
Sbjct: 1164 IEVSIPDSVPVLKVQEASMPVQKLERGGCKDDTTLETELKNIEVQEPKKPSDKKTKKQKS 1223

Query: 1216 SKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSSDTSSSPQKTRDG-DAK 1275
            SK LSSDQ KDSKNSAIQQSKQSKSGK ENDLKLKADNI+GKSSD +SSP+K RDG D K
Sbjct: 1224 SKSLSSDQAKDSKNSAIQQSKQSKSGKSENDLKLKADNIMGKSSDLASSPRKIRDGDDGK 1283

Query: 1276 IAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWKVASSFKPK 1335
            I++VD QP+QSSAS++N+W+D +TVQVK+D+RL+GSDSV NSQ QS QRAWKVASSFKPK
Sbjct: 1284 ISVVDHQPIQSSASAMNTWSDGDTVQVKDDARLVGSDSVLNSQTQSAQRAWKVASSFKPK 1343

Query: 1336 SLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHKDSVNSEPS 1395
            SLLEIQEEEQK AHTETAVSEISTSI S+SLSTPWAGIVS+ DPKAS+EIHKDSV SE S
Sbjct: 1344 SLLEIQEEEQKRAHTETAVSEISTSITSMSLSTPWAGIVSSSDPKASKEIHKDSVISESS 1403

Query: 1396 EKHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSDSAQIASSPQVMAAQAEPMDDNFI 1455
            EKHE+LL S+ R+SQLHDLLAED+MEKSGA DVRVSDS QIASSP+V+A QAEPMDDNFI
Sbjct: 1404 EKHENLLISKIRRSQLHDLLAEDNMEKSGASDVRVSDSVQIASSPRVLATQAEPMDDNFI 1463

Query: 1456 EAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKDAMPAIPSG 1515
            EAKDTKKSRKKSAKAK VG+K ++ VPS DVPVGSSP EKGKISRQTQQEK+AMPAIPSG
Sbjct: 1464 EAKDTKKSRKKSAKAKGVGSKPSAPVPSGDVPVGSSPNEKGKISRQTQQEKEAMPAIPSG 1523

Query: 1516 PSFGDFVLWKGEAANVAPAPAW-SSDSGKVPKPTSLRDIQKEQERKISSAQHSHQIPTPQ 1575
            PSFGDFVLWKGE ANVAP+PAW SSDSGKVPKPTSLRDIQKEQ RK S+AQHSHQIPTPQ
Sbjct: 1524 PSFGDFVLWKGEVANVAPSPAWSSSDSGKVPKPTSLRDIQKEQGRKTSAAQHSHQIPTPQ 1583

Query: 1576 KAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGPIESKQENQ 1635
            K QP+QVGRSSSTSTPSWALSASSPSKAASSPLQNVP+QS +GGDDDLFWGPIESK+ENQ
Sbjct: 1584 KGQPSQVGRSSSTSTPSWALSASSPSKAASSPLQNVPTQSNHGGDDDLFWGPIESKKENQ 1643

Query: 1636 QVDVRLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDSVTKH 1695
            QVDVRL S+ +WGNRN PAK AASTG+LSRQKSSGGKADYLSSSPAQSSQKGKQD VTKH
Sbjct: 1644 QVDVRLVSN-NWGNRNAPAK-AASTGVLSRQKSSGGKADYLSSSPAQSSQKGKQDPVTKH 1703

Query: 1696 SEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQFLN 1755
            SEAMGFRDWCESEC RLIG KDTSFLEFCLKQSRSEAEL LIENLGSYDPDH+FIDQFLN
Sbjct: 1704 SEAMGFRDWCESECERLIGIKDTSFLEFCLKQSRSEAELYLIENLGSYDPDHDFIDQFLN 1763

Query: 1756 FKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAGGDLDPDVP-GRDGSAKGGGKKKGK 1815
            +K+LLPADVLEIAFQSRNDRKVSA+A+REVNSGNAGGDLDPDVP GRDGSAK GGKKKGK
Sbjct: 1764 YKDLLPADVLEIAFQSRNDRKVSAVASREVNSGNAGGDLDPDVPVGRDGSAKSGGKKKGK 1823

Query: 1816 KGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            KGKKV+PSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 KGKKVNPSVLGFNVVSNRIMMGEIQTVED 1847

BLAST of Spg033765 vs. ExPASy TrEMBL
Match: A0A6J1K6T8 (uncharacterized protein LOC111491700 OS=Cucurbita maxima OX=3661 GN=LOC111491700 PE=4 SV=1)

HSP 1 Score: 3030.7 bits (7856), Expect = 0.0e+00
Identity = 1592/1832 (86.90%), Postives = 1691/1832 (92.30%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            +VQGSENPIPLSPQWLLPKPGESKHG+GTGENHF+ QP YGN MD MKGSEN EDMNEIQ
Sbjct: 24   DVQGSENPIPLSPQWLLPKPGESKHGMGTGENHFSHQPVYGNRMDTMKGSENSEDMNEIQ 83

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKKE+FRPSLTD+EIGRRDRWHDEEREN+SS+RKDRWRDGEKE GD RKMDRW EDSS R
Sbjct: 84   KKKEIFRPSLTDSEIGRRDRWHDEERENSSSVRKDRWRDGEKEIGDGRKMDRWNEDSSTR 143

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
            VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKR+DSGRDGDLH
Sbjct: 144  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRVDSGRDGDLH 203

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSHRGRADN 255
            LDKNFSHVSNYGKNDRDGDHYRPWRSS+SQGRGKGEP HHQTQTPIKQV AFSHRGRADN
Sbjct: 204  LDKNFSHVSNYGKNDRDGDHYRPWRSSSSQGRGKGEPPHHQTQTPIKQVPAFSHRGRADN 263

Query: 256  TPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKLLDVYRTTNLT 315
            TPPT+S+GRGIISSGVNP NSIYSS +SLGASSEKSGREPY+YKYSRTKLLDV+RTT+LT
Sbjct: 264  TPPTFSLGRGIISSGVNPPNSIYSSPHSLGASSEKSGREPYYYKYSRTKLLDVFRTTSLT 323

Query: 316  SQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 375
            SQ  +KDGFVPVPTLTLD+PLEPLALC PT EEMTFLKGIDKGEIVSSGAPQVSKDGRNS
Sbjct: 324  SQQTLKDGFVPVPTLTLDEPLEPLALCVPTTEEMTFLKGIDKGEIVSSGAPQVSKDGRNS 383

Query: 376  SEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTERQVPYHRP 435
            SEF QTRRTKLGVSPS GSREDL HGFDD NDDKDDSTTKPGHTNYSEV+TERQ+PYHRP
Sbjct: 384  SEFMQTRRTKLGVSPSLGSREDLPHGFDDCNDDKDDSTTKPGHTNYSEVTTERQLPYHRP 443

Query: 436  QSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGSTVHPGSTWDASS 495
                      +AHASS FKSEAFREDDNAMRKADE PV+RESSVKGG+ V  G TWDASS
Sbjct: 444  ----------LAHASSTFKSEAFREDDNAMRKADEGPVSRESSVKGGTNVQHGRTWDASS 503

Query: 496  LEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKDNPKWQTGEE 555
            LEQ  NTS+PDWRDNPNNI+SGTPDKG LQ SKNLNDGWGSN+ATPSYPK+NPKW TG+E
Sbjct: 504  LEQLLNTSLPDWRDNPNNINSGTPDKG-LQSSKNLNDGWGSNSATPSYPKENPKWHTGDE 563

Query: 556  SIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQWFEGGYFGLD 615
            SI+RRQLSGILDKEQLARKT QSAPEDLQ HYIDPSGAIQGPFSGADIIQWFEGGYFGLD
Sbjct: 564  SILRRQLSGILDKEQLARKTVQSAPEDLQFHYIDPSGAIQGPFSGADIIQWFEGGYFGLD 623

Query: 616  LPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASYGSLGKLHTG 675
            LPVR AN PN+LPFSALGD MPHLRSKAKPPPGF GPK NEFADT+GNAS GSLGKLHTG
Sbjct: 624  LPVRLANAPNELPFSALGDAMPHLRSKAKPPPGFCGPKQNEFADTLGNASIGSLGKLHTG 683

Query: 676  LNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGYFGNNPNNLS 735
            LNEID LRNE RHKHGSTVEAENRFLESLMSGN+GSSPLEKSAFSEG+PGYFGNN +NL 
Sbjct: 684  LNEIDPLRNETRHKHGSTVEAENRFLESLMSGNIGSSPLEKSAFSEGLPGYFGNNSSNLP 743

Query: 736  SLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDPIQQAKLLSS 795
            SLGIDNGNNLFLLAKRMELERQRSL+NPYAFWPGID +SKVSKPDIGLDDPIQQAKLLSS
Sbjct: 744  SLGIDNGNNLFLLAKRMELERQRSLSNPYAFWPGIDASSKVSKPDIGLDDPIQQAKLLSS 803

Query: 796  IIDHSRQTSHSQGADMSAILQGLSDKAPPGINDVAGWSKFASQCASDPLQSKLDLHHDLN 855
            IIDHSRQTSH Q ADMSAILQGLSDKAPPGINDVAGWSKFASQCA DPLQSKLDLHHDLN
Sbjct: 804  IIDHSRQTSHPQSADMSAILQGLSDKAPPGINDVAGWSKFASQCAPDPLQSKLDLHHDLN 863

Query: 856  LSSQAPFGFQQQRLPPQPSLTNLLAQATDNPTLTPDKFLPSSLSQDPQLISKLQQQHLLQ 915
            LSSQAPFGFQQQRL PQP LTNLLAQATDN TLT DKFLPSSLSQDPQLISKLQQQHLLQ
Sbjct: 864  LSSQAPFGFQQQRLQPQPPLTNLLAQATDNSTLTSDKFLPSSLSQDPQLISKLQQQHLLQ 923

Query: 916  LHSQVPFSAQQMSLLDKLLLLKQQQKQEEQQQLL--QQQQLLSQVLSEHQSRQHFGDPSF 975
            LHSQVPFSAQQMSLLDK+LLLKQQQKQEEQQQLL  QQQQLLSQVLSEHQS QHF DPSF
Sbjct: 924  LHSQVPFSAQQMSLLDKILLLKQQQKQEEQQQLLQQQQQQLLSQVLSEHQSHQHFVDPSF 983

Query: 976  GQLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVLTDRATTFGNMALQVTQGPSYNV 1035
            GQL GA IPIGNAS DPSQVQLSREKFQIGSQ PLNVLTD ATT+GNMALQ TQG SYNV
Sbjct: 984  GQLHGASIPIGNASNDPSQVQLSREKFQIGSQKPLNVLTDHATTYGNMALQATQGASYNV 1043

Query: 1036 NSEDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSGSKVGEGSVLPGLTSKSNE 1095
            NSEDPS++LP QMFGNVVQQKSW AA+PEQLND  PKD++ GS V EGS+LPG++SKSNE
Sbjct: 1044 NSEDPSLILPPQMFGNVVQQKSWTAAIPEQLNDTRPKDVIPGSNVVEGSLLPGMSSKSNE 1103

Query: 1096 DVNLVPTSSDSHT-IKALEQISEDVPRLDATDTSFA-PDAIVEPLPLKTDEVSVAIPPSE 1155
            DVNLVP SSDSHT IK LE+ISEDVPRLDAT TSFA  DA VEPLPLK  E+SVAIPP+ 
Sbjct: 1104 DVNLVPKSSDSHTIIKTLEKISEDVPRLDATVTSFASDDATVEPLPLKNAEISVAIPPAA 1163

Query: 1156 VRN---SIPVSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEVQEPKKSSDKKTKK 1215
            V N   S+P SVP VKV+EA MP+EKL RD  +D+TSLE E+KNVEVQEP+KSSDKKTKK
Sbjct: 1164 VHNIEISVPNSVPAVKVQEAIMPMEKLARDGSRDETSLEAEVKNVEVQEPRKSSDKKTKK 1223

Query: 1216 QKSSKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSSDTSSSPQKTRDGD 1275
            QKSSKLLSSDQ KD KNS IQQSKQSKSGK ENDLKLK+DNIVGKSSDT+ SP+K RDGD
Sbjct: 1224 QKSSKLLSSDQAKDFKNSGIQQSKQSKSGKSENDLKLKSDNIVGKSSDTACSPRKIRDGD 1283

Query: 1276 AKIAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQSGQRAWKVASSFK 1335
             KIAIVDSQ  QSSAS++NSWND ETV V+++SRL GSDSV NSQ QSGQRAWK+AS+FK
Sbjct: 1284 GKIAIVDSQLDQSSASAVNSWNDGETVPVRDESRLSGSDSVLNSQTQSGQRAWKIASNFK 1343

Query: 1336 PKSLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPKASREIHKDSVNSE 1395
            PKSLLEIQEEEQKMAHTET VS+ISTSIN++SLSTPWAGIVS+ DPKASREIHKDSVNSE
Sbjct: 1344 PKSLLEIQEEEQKMAHTETVVSDISTSINTMSLSTPWAGIVSSSDPKASREIHKDSVNSE 1403

Query: 1396 PSEKHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSDSAQIASSPQVMAAQAEPMDDN 1455
             SEKHE+LL SRSRKSQLHDLLAEDDMEKSGAGDVRVSD+ QIASSPQVMA +AEPMD+N
Sbjct: 1404 SSEKHENLLTSRSRKSQLHDLLAEDDMEKSGAGDVRVSDTVQIASSPQVMAVRAEPMDEN 1463

Query: 1456 FIEAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGKISRQTQQEKDAMPAIP 1515
            FIEAKDTKKSRKKSAKAK +GTKA+S+VPSADVPVG SP+EKGKISRQ QQEK+AMPAIP
Sbjct: 1464 FIEAKDTKKSRKKSAKAKGIGTKASSAVPSADVPVGLSPVEKGKISRQMQQEKEAMPAIP 1523

Query: 1516 SGPSFGDFVLWKGEAANVAPAPAWSSDSGKVPKPTSLRDIQKEQERKISSAQHSHQIPTP 1575
            SGPSFGDFVLWKGE ANVAPA AWSSDSGKV KPTSLRDIQ EQERKIS+AQH  QI TP
Sbjct: 1524 SGPSFGDFVLWKGEVANVAPALAWSSDSGKVAKPTSLRDIQMEQERKISAAQHPQQISTP 1583

Query: 1576 QKAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQSKYGGDDDLFWGPIESKQEN 1635
            QKAQPTQVGRSS T+TPSW+LSASSPSKAAS+PLQN+ +QSK+GGDDDLFWGPIESKQEN
Sbjct: 1584 QKAQPTQVGRSSRTTTPSWSLSASSPSKAASTPLQNIATQSKHGGDDDLFWGPIESKQEN 1643

Query: 1636 QQVDVRLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKADYLSSSPAQSSQKGKQDSVTK 1695
            Q+VDVR GSHG+WGNRNTP KA ASTGLLSRQKSSGGKAD+LSSSPAQSSQKGKQD +TK
Sbjct: 1644 QRVDVRPGSHGNWGNRNTPVKAVASTGLLSRQKSSGGKADHLSSSPAQSSQKGKQDPITK 1703

Query: 1696 HSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQFL 1755
            HSEAMGFRDWCE+ECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQFL
Sbjct: 1704 HSEAMGFRDWCENECVRLIGTKDTSFLEFCLKQSRSEAELLLIENLGSYDPDHEFIDQFL 1763

Query: 1756 NFKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNA-GGDLDPDV-PGRDGSAK-GGGKK 1815
            N+KELLPAD++EIAFQSRN+RKVSA+A+R+VNSGNA GGDLDPDV  GRDGSAK GGGKK
Sbjct: 1764 NYKELLPADIIEIAFQSRNERKVSAMASRDVNSGNAGGGDLDPDVSAGRDGSAKSGGGKK 1823

Query: 1816 KGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            KGKKGKK++PSVLGFNVVSNRIMMGEIQTVED
Sbjct: 1824 KGKKGKKINPSVLGFNVVSNRIMMGEIQTVED 1844

BLAST of Spg033765 vs. TAIR 10
Match: AT5G42950.1 (GYF domain-containing protein )

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 816/1855 (43.99%), Postives = 1117/1855 (60.22%), Query Frame = 0

Query: 16   NVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMDMMKGSENYEDMNEIQ 75
            ++QGS+N IPLSPQWLL KPGE+K G+GTG+ +      YGNH D+++ + N E+  +  
Sbjct: 28   DIQGSDNAIPLSPQWLLSKPGENKTGMGTGDPN-----QYGNHSDVVRTTGNGEETLDNL 87

Query: 76   KKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDRWTEDSSAR 135
            KKK+VFRPSL DAE GRRDRW DEER+  SS+R DRWR+G+K++GD++K+DRW  D+ A 
Sbjct: 88   KKKDVFRPSLLDAESGRRDRWRDEERDTLSSVRNDRWRNGDKDSGDNKKVDRW--DNVAP 147

Query: 136  VFRESRRGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRMDSGRDGDLH 195
             F E RRGP++RW+DS N+D    +QRRESKWN+RWGPDDKE E  R K  + G+DG++ 
Sbjct: 148  KFGEQRRGPNDRWTDSGNKDAAP-EQRRESKWNSRWGPDDKEAEIPRNKWDEPGKDGEII 207

Query: 196  LDKNFSHVSNYGKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVAAFSH-RGRAD 255
             +K  S  ++      DGDHYRPWR   SQGRG+GE  H+Q+ TP KQV +FSH RGR +
Sbjct: 208  REKGPSLPTS------DGDHYRPWR--PSQGRGRGEALHNQS-TPNKQVTSFSHSRGRGE 267

Query: 256  NTPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEK--SG-REPYHYKYSRTKLLDVYRT 315
            NT   +S GRG +S G +   S  + S+  G++S+K  SG  EP H +YSR KLLDVYR 
Sbjct: 268  NT-AIFSAGRGRMSPGGSIFTSAPNQSHPPGSASDKGESGPGEPPHLRYSRMKLLDVYRM 327

Query: 316  TNLTSQPGVKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSGAPQVSKD 375
             +        DGF+ VP+LT ++P +PLALCAP+++E+  L  I+KG+IVSSGAPQ SKD
Sbjct: 328  ADTECYEKFPDGFIEVPSLTSEEPTDPLALCAPSSDEVNVLDAIEKGKIVSSGAPQTSKD 387

Query: 376  ---GRNSSEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEVSTER 435
               GRN  EF+Q RR +       GSRED++ G ++  D+  ++   P            
Sbjct: 388  GPTGRNPVEFSQPRRIR-----PAGSREDMTFGAEESKDESGETRNYP------------ 447

Query: 436  QVPYHRPQSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVN--RESSVKGGSTVH 495
                                   KF+ EA  E     R+ +E PV   +E S++G + V 
Sbjct: 448  ---------------------DDKFRPEASHEGYAPFRRGNEAPVRELKEPSMQGNAHVQ 507

Query: 496  PGSTWDASSLEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTATPSYPKD 555
              S W  SS  +  N +  DW D   +    + D  W  P  ++N   G+N   P   K 
Sbjct: 508  SASPWRQSSGGERSNRNSHDWNDPSADSRLKSSDSVWSHPKDSINHLGGNNMMLPQ-SKG 567

Query: 556  NPKWQTGEESIIRRQLSGILDKEQLARKTYQSAPEDLQLHYIDPSGAIQGPFSGADIIQW 615
              +WQ  E+  +RRQ S + D+EQ  RK   S+PE+L L+Y DP G IQGPFSG+DII W
Sbjct: 568  ESRWQISEDPSLRRQPSLVFDREQEVRKLLPSSPEELSLYYKDPQGLIQGPFSGSDIIGW 627

Query: 616  FEGGYFGLDLPVRPANPPNDLPFSALGDVMPHLRSKAKPPPGFSGPKPNEFADTIGNASY 675
            FE GYFG+DL VR A+ PND PFS LGDVMPHLR+K+ PPPGF+G K NEF D  G +++
Sbjct: 628  FEAGYFGIDLLVRLASAPNDSPFSLLGDVMPHLRAKSGPPPGFTGAKQNEFVDAAGTSAF 687

Query: 676  GSLGKLHTGLNEIDTLRNEARHKHGSTVEAENRFLESLMSGNLGSSPLEKSAFSEGVPGY 735
              +GK+H+G+ E D L+N+ R+KH +   AENRF+ESLMSG L +S       ++GV GY
Sbjct: 688  PGVGKVHSGMGETDMLQNDMRYKHVAGTVAENRFIESLMSGGLTNS-------AQGVQGY 747

Query: 736  FGNNPNNLSSLGIDNGNNLFLLAKRMELERQRSLTNPYAFWPGIDPTSKVSKPDIGLDDP 795
              N+   LS    D G +++LLAK++ELERQRS+ +PY++WPG +  + +   +      
Sbjct: 748  GVNSSGGLSLPVTDGGADMYLLAKKLELERQRSIPSPYSYWPGRESANLMPGSE------ 807

Query: 796  IQQAKLLSSIIDHSRQTSHSQGADMSAILQGLSDKAPPGIN-DVAGWSKFASQCASDPLQ 855
                    ++ ++++Q + S  +D+ +ILQG++D++ P ++  +  WS+        P+Q
Sbjct: 808  --------NVSENAQQPTRSPSSDLLSILQGVTDRSSPAVSGPLPAWSQ--------PIQ 867

Query: 856  SKLDLHHDLNLSSQAPFGFQQQRLPPQP-SLTNLLAQATDN---PTLTPDKFLPSSLSQD 915
             + DLHH     +Q PFG QQQRLP Q   L+ LL Q  +N     L+PD  L + LSQ+
Sbjct: 868  KESDLHHAKTFQTQIPFGVQQQRLPEQNLPLSGLLGQPMENNPGGMLSPDMMLAAGLSQE 927

Query: 916  PQLISKLQQQH-LLQLHSQVPFSAQ-QMSLLDKLLLLKQQQKQEEQQQLL-QQQQLLSQV 975
             Q ++ LQQQ  LLQL++Q P SAQ Q  L++K+LLLK Q KQEEQQQLL QQQQL SQV
Sbjct: 928  HQSLNLLQQQQLLLQLNAQTPLSAQHQRLLVEKMLLLKHQHKQEEQQQLLRQQQQLYSQV 987

Query: 976  LSEHQ-SRQHFGDPSFGQLQGAPIPIGNASADPSQVQLSREKFQIGSQNPLNVL-TDRAT 1035
             ++ Q S+Q FGDPS+GQLQ        AS D  ++Q S++  Q+  Q  + V   +R  
Sbjct: 988  FADQQRSQQRFGDPSYGQLQ--------ASLDALRLQPSKDMSQVNQQVQVPVSHEERGI 1047

Query: 1036 TFGNMALQVTQGPSYNVNS-EDPSVVLPHQMFGNVVQQKSWNAALPEQLNDIHPKDILSG 1095
               ++ L VT   +  V S E PS+ L +Q+FGNV  +      LP+Q++D H K+  S 
Sbjct: 1048 NLADL-LPVTHATNQTVASFETPSLHLQNQLFGNVDPR----MVLPDQIDDTHKKE--SK 1107

Query: 1096 SKVGEGSVLPGLTSKSNEDVNLVPTSSDSHTIKALEQISEDVPRLDATDTSFAPDAIVEP 1155
            S+         + S  +E   L P    +H ++  E +S   P  +++  +     IVE 
Sbjct: 1108 SEYERTVSADYVNSLYSEKPVLSPGYHATHNVE--EPVS--YPNNESSTATMTAPEIVES 1167

Query: 1156 LPLKTDEVSVAIPPSEVRNSIPVSVPVVKVEEASMPVEKLERDVCKDDTSLETELKNVEV 1215
              L+     +     EV   +    P                          TE+KN +V
Sbjct: 1168 KLLEEQSKDMYAGKGEVSIELSGETPA-------------------------TEVKNNDV 1227

Query: 1216 QEPKKSSDKKTKKQKSSKLLSSDQVKDSKNSAIQQSKQSKSGKPENDLKLKADNIVGKSS 1275
               +K+S+KK++KQ++ +  ++D  K +  + +Q++K+ + G  ++        I GK  
Sbjct: 1228 SVARKTSEKKSRKQRAKQ--AADLAKSTSRAPLQETKKPQPGSADD------SEIKGK-- 1287

Query: 1276 DTSSSPQKTRDGDAKIAIVDSQPVQSSASSINSWNDVETVQVKEDSRLIGSDSVPNSQIQ 1335
             T  S     D D  + I  S    S+ S ++S  +V++V+ +E        S+ N++ Q
Sbjct: 1288 -TKKSADTLIDNDTHL-IKSSTATASNTSQMSS--EVDSVRGEE-------SSLQNTRTQ 1347

Query: 1336 SGQRAWKVASSFKPKSLLEIQEEEQKMAHTETAVSEISTSINSVSLSTPWAGIVSNLDPK 1395
             G RAWK A  FKPKSLLEIQ EEQ++A  E    +IS+++NSV  + PWAGIV+N D  
Sbjct: 1348 PG-RAWKPAPGFKPKSLLEIQMEEQRVAQAEALAPKISSTVNSVGSAAPWAGIVTNSDSN 1407

Query: 1396 ASREIHKDSVNSEPS-EKHESLLNSRSRKSQLHDLLAEDDMEKSGAGDVRVSD-SAQIAS 1455
              RE H +S  ++    K ES+   +++KS LHDLLA+D   KS   +  V +  +   +
Sbjct: 1408 ILRETHGESAITQTGVVKPESVPTLKAKKSHLHDLLADDVFAKSSDKEREVMEIISNNDA 1467

Query: 1456 SPQVMAAQAEPM-DDNFIEAKDTKKSRKKSAKAKAVGTKATSSVPSADVPVGSSPIEKGK 1515
              QV    AE   DDNFI+A++TKKSRKKSA+AK  G K  + VP+ D  + ++ +EKGK
Sbjct: 1468 FMQVTTTNAESFDDDNFIDARETKKSRKKSARAKTSGAKIAAHVPAVDTSLQTNSVEKGK 1527

Query: 1516 ISR-QTQQEKDAMPAIPSGPSFGDFVLWKGEAA-NVAPAPAWSSDSGKVPKPTSLRDIQK 1575
             SR   QQEK+ +PAIPSGPS GDFVLWKGE+  N  PA AWSS   K  KP+SLRDI K
Sbjct: 1528 SSRILQQQEKEVLPAIPSGPSLGDFVLWKGESVNNPPPAAAWSSGPKKSTKPSSLRDIVK 1587

Query: 1576 EQERKISSAQ-HSHQIPTPQKAQPTQVGRSSSTSTPSWALSASSPSKAASSPLQNVPSQS 1635
            EQE+  +S+      +PT QKA P Q  +  +    SW+ SASSPS+A S       SQS
Sbjct: 1588 EQEKMTTSSHPPPSPVPTTQKAIPPQAHQGGA----SWSRSASSPSQAVSQS----SSQS 1647

Query: 1636 KYGGDDDLFWGPIE-SKQENQQVDV-RLGSHGSWGNRNTPAKAAASTGLLSRQKSSGGKA 1695
            K  GDDDLFWGP+E S Q+ +Q D   L S  SWG +NTP K  A T L  ++  S G A
Sbjct: 1648 KSKGDDDLFWGPVEQSTQDTKQGDFPHLTSQNSWGTKNTPGKVNAGTSLNRQKSVSMGSA 1707

Query: 1696 DYLSSSP--AQSSQKGKQDSVTKHSEAMGFRDWCESECVRLIGTKDTSFLEFCLKQSRSE 1755
            D + SSP   Q+S KGK+++VTK +EA GFRDWC+SEC+RL+G++DTS LEFCLK SRSE
Sbjct: 1708 DRVLSSPVVTQASHKGKKEAVTKLTEANGFRDWCKSECLRLLGSEDTSVLEFCLKLSRSE 1714

Query: 1756 AELLLIENLGSYDPDHEFIDQFLNFKELLPADVLEIAFQSRNDRKVSAIAAREVNSGNAG 1815
            AE LLIENLGS DPDH+FID+FLN+K+LLP++V+EIAFQS    K S +  R     N G
Sbjct: 1768 AETLLIENLGSRDPDHKFIDKFLNYKDLLPSEVVEIAFQS----KGSGVGTR----NNTG 1714

Query: 1816 GDLDPD-VPGRDGSAKGGGKKKGKKGKKV--SPSVLGFNVVSNRIMMGEIQTVED 1838
             D   +     DG +K GGKKK KKGKKV  S SVLGFNVVSNRIMMGEIQT+ED
Sbjct: 1828 EDYYYNTTAANDGFSKVGGKKKAKKGKKVSLSASVLGFNVVSNRIMMGEIQTIED 1714

BLAST of Spg033765 vs. TAIR 10
Match: AT1G24300.1 (GYF domain-containing protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.1e-31
Identity = 186/648 (28.70%), Postives = 273/648 (42.13%), Query Frame = 0

Query: 10  ESNKTVNVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMD--MMKGSEN 69
           +S+  +    S+N IPLSPQWL  K  ESK  + +        P+  N  D   +   E+
Sbjct: 16  KSSDQLKELASDNSIPLSPQWLYTKSSESKMDVRSPTPMPMGNPSDPNLKDAWRLDAPED 75

Query: 70  YEDMNEIQKKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDR 129
            +D  +I  + E  R            RW +EERE            G ++  D RK +R
Sbjct: 76  KKDWKKIVSENETNR------------RWREEERETGLL--------GARKV-DRRKTER 135

Query: 130 WTEDSSARVFRESR-RGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRM 189
             ++ S+R   E +    S+RW+D ++R  VH + RR++KW++RWGPDDKE E  R +++
Sbjct: 136 RIDNVSSRETGEVKTTAASDRWNDVNSRAAVH-EPRRDNKWSSRWGPDDKEKEA-RCEKV 195

Query: 190 DSGRDGDLHLDKNFSHVSNY-GKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVA 249
           +  +D +    ++ S VSN    ++RD D    WR         G PT ++T        
Sbjct: 196 EINKDKEEPQSESQSVVSNVRATSERDSDPRDKWRPRHRMESQSGVPTSYRTAPGFG--- 255

Query: 250 AFSHRGRADNTPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKL 309
               RGRA+     +++GRG  S     T    SS++ +GA S  +      ++Y R KL
Sbjct: 256 --LDRGRAEGPNLGFTVGRGRAS-----TIGRGSSTSLIGAGSASAP----VFRYPRGKL 315

Query: 310 LDVYRTTNLTSQPGVKDGFVP-----VPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEI 369
           LD+YR      +P    G +P     V ++T    +EPLA  AP  EE   + GI KG I
Sbjct: 316 LDMYR----KQKPDPSLGRIPTEMDEVASITQVALIEPLAFIAPDTEEEASINGIWKGRI 375

Query: 370 VSSGAPQVSKDGRNSSEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTN 429
           +S            S  +T +    LG                      ++S  K     
Sbjct: 376 IS------------SEVYTSSGEESLG----------------------ENSLLKCRIPE 435

Query: 430 YSEVSTERQVPYHRPQSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVK 489
             E   +  +                           F   DN   K      N +S + 
Sbjct: 436 SGETKVDGAL-------------------------LGFMNGDNGSMK------NNDSGLL 495

Query: 490 GGSTVHPGSTWDASSLEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTAT 549
           G    H G    ASS+  PR  S+        + S G+   G+           GS  A 
Sbjct: 496 GS---HNGGLGAASSV--PRLNSVA-------SESYGSFGAGY-------QVSHGSPEAV 537

Query: 550 PSYPKDNPKWQTGEESII---RRQLSGILDKEQLARKTYQSA--PEDLQLHYIDPSGAIQ 609
            S    +P    G ES++    +   G L +  +     ++A  PED    YIDP G IQ
Sbjct: 556 RSVFTKSPVLD-GSESVVGSFEQDYMGKLQQPDVEVDQSEAAMPPEDFLFLYIDPQGVIQ 537

Query: 610 GPFSGADIIQWFEGGYFGLDLPVRPANPPNDLPFSALGDVMPHLRSKA 644
           GPF G+DII WFE G+FG DL VR AN P   PF  LG VM +L++++
Sbjct: 616 GPFIGSDIISWFEQGFFGTDLQVRLANAPEGTPFQDLGRVMSYLKTES 537

HSP 2 Score: 51.2 bits (121), Expect = 1.0e-05
Identity = 26/42 (61.90%), Postives = 33/42 (78.57%), Query Frame = 0

Query: 1796 DGSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            +GS  GGGKKKGKKG+++ P++LGF V SNRI MGEI   +D
Sbjct: 1454 EGSKGGGGKKKGKKGRQIDPALLGFKVTSNRI-MGEIHRADD 1494

BLAST of Spg033765 vs. TAIR 10
Match: AT1G24300.2 (GYF domain-containing protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.1e-31
Identity = 186/648 (28.70%), Postives = 273/648 (42.13%), Query Frame = 0

Query: 10  ESNKTVNVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMD--MMKGSEN 69
           +S+  +    S+N IPLSPQWL  K  ESK  + +        P+  N  D   +   E+
Sbjct: 16  KSSDQLKELASDNSIPLSPQWLYTKSSESKMDVRSPTPMPMGNPSDPNLKDAWRLDAPED 75

Query: 70  YEDMNEIQKKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDR 129
            +D  +I  + E  R            RW +EERE            G ++  D RK +R
Sbjct: 76  KKDWKKIVSENETNR------------RWREEERETGLL--------GARKV-DRRKTER 135

Query: 130 WTEDSSARVFRESR-RGPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRM 189
             ++ S+R   E +    S+RW+D ++R  VH + RR++KW++RWGPDDKE E  R +++
Sbjct: 136 RIDNVSSRETGEVKTTAASDRWNDVNSRAAVH-EPRRDNKWSSRWGPDDKEKEA-RCEKV 195

Query: 190 DSGRDGDLHLDKNFSHVSNY-GKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVA 249
           +  +D +    ++ S VSN    ++RD D    WR         G PT ++T        
Sbjct: 196 EINKDKEEPQSESQSVVSNVRATSERDSDPRDKWRPRHRMESQSGVPTSYRTAPGFG--- 255

Query: 250 AFSHRGRADNTPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKL 309
               RGRA+     +++GRG  S     T    SS++ +GA S  +      ++Y R KL
Sbjct: 256 --LDRGRAEGPNLGFTVGRGRAS-----TIGRGSSTSLIGAGSASAP----VFRYPRGKL 315

Query: 310 LDVYRTTNLTSQPGVKDGFVP-----VPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEI 369
           LD+YR      +P    G +P     V ++T    +EPLA  AP  EE   + GI KG I
Sbjct: 316 LDMYR----KQKPDPSLGRIPTEMDEVASITQVALIEPLAFIAPDTEEEASINGIWKGRI 375

Query: 370 VSSGAPQVSKDGRNSSEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTN 429
           +S            S  +T +    LG                      ++S  K     
Sbjct: 376 IS------------SEVYTSSGEESLG----------------------ENSLLKCRIPE 435

Query: 430 YSEVSTERQVPYHRPQSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVK 489
             E   +  +                           F   DN   K      N +S + 
Sbjct: 436 SGETKVDGAL-------------------------LGFMNGDNGSMK------NNDSGLL 495

Query: 490 GGSTVHPGSTWDASSLEQPRNTSMPDWRDNPNNISSGTPDKGWLQPSKNLNDGWGSNTAT 549
           G    H G    ASS+  PR  S+        + S G+   G+           GS  A 
Sbjct: 496 GS---HNGGLGAASSV--PRLNSVA-------SESYGSFGAGY-------QVSHGSPEAV 537

Query: 550 PSYPKDNPKWQTGEESII---RRQLSGILDKEQLARKTYQSA--PEDLQLHYIDPSGAIQ 609
            S    +P    G ES++    +   G L +  +     ++A  PED    YIDP G IQ
Sbjct: 556 RSVFTKSPVLD-GSESVVGSFEQDYMGKLQQPDVEVDQSEAAMPPEDFLFLYIDPQGVIQ 537

Query: 610 GPFSGADIIQWFEGGYFGLDLPVRPANPPNDLPFSALGDVMPHLRSKA 644
           GPF G+DII WFE G+FG DL VR AN P   PF  LG VM +L++++
Sbjct: 616 GPFIGSDIISWFEQGFFGTDLQVRLANAPEGTPFQDLGRVMSYLKTES 537

HSP 2 Score: 51.2 bits (121), Expect = 1.0e-05
Identity = 26/42 (61.90%), Postives = 33/42 (78.57%), Query Frame = 0

Query: 1796 DGSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            +GS  GGGKKKGKKG+++ P++LGF V SNRI MGEI   +D
Sbjct: 1449 EGSKGGGGKKKGKKGRQIDPALLGFKVTSNRI-MGEIHRADD 1489

BLAST of Spg033765 vs. TAIR 10
Match: AT1G27430.1 (GYF domain-containing protein )

HSP 1 Score: 126.7 bits (317), Expect = 1.9e-28
Identity = 177/646 (27.40%), Postives = 264/646 (40.87%), Query Frame = 0

Query: 10  ESNKTVNVQGSENPIPLSPQWLLPKPGESKHGIGTGENHFNQQPAYGNHMD--MMKGSEN 69
           +S+  +    S+N IPLSPQWL  K  E K  + +        P+  N  D   +   E+
Sbjct: 16  KSSDQLKELASDNSIPLSPQWLYTKSSEYKMDVRSPTPVPMGNPSDPNPKDAWRLDAPED 75

Query: 70  YEDMNEIQKKKEVFRPSLTDAEIGRRDRWHDEERENNSSMRKDRWRDGEKEAGDSRKMDR 129
            +D  +I  + E  R            RW +EERE            G ++  D RK +R
Sbjct: 76  KKDWKKIVHENETSR------------RWREEERETGLL--------GARKV-DRRKTER 135

Query: 130 WTEDSSARVFRESRR-GPSERWSDSSNRDNVHYDQRRESKWNTRWGPDDKETEGFREKRM 189
             +  S+R   + +    S+RW+D ++R  VH + RR++KW++RWGPDDKE E  R +++
Sbjct: 136 RIDSVSSRETGDIKNAAASDRWNDVNSRAAVH-EPRRDNKWSSRWGPDDKEKEA-RCEKV 195

Query: 190 DSGRDGDLHLDKNFSHVSNY-GKNDRDGDHYRPWRSSASQGRGKGEPTHHQTQTPIKQVA 249
           D  +D +    ++ S VSN    ++RD D    WR         G P+ ++         
Sbjct: 196 DINKDKEEPQSESQSVVSNVRATSERDSDTRDKWRPRHRMESQSGGPSSYRAAPGFG--- 255

Query: 250 AFSHRGRADNTPPTYSIGRGIISSGVNPTNSIYSSSNSLGASSEKSGREPYHYKYSRTKL 309
               RGRA+     +++GRG  S     T    SS++ +GA S  S      ++Y R KL
Sbjct: 256 --LDRGRAEGPNLGFTVGRGRAS-----TIGRGSSTSLIGAGSALSP----VFRYPRGKL 315

Query: 310 LDVYRTTNLTSQPG-VKDGFVPVPTLTLDDPLEPLALCAPTAEEMTFLKGIDKGEIVSSG 369
           LD+YR     S  G +      V ++T    +EPLA  AP AEE   L GI KG I+S  
Sbjct: 316 LDMYRKQKPDSSLGRILTEMDEVASITQVALIEPLAFIAPDAEEEANLNGIWKGRIIS-- 375

Query: 370 APQVSKDGRNSSEFTQTRRTKLGVSPSPGSREDLSHGFDDYNDDKDDSTTKPGHTNYSEV 429
                     S  +T +    L      G    L     +  + K D             
Sbjct: 376 ----------SEVYTSSGEESL------GGNSLLKCRIPESGETKVDGALL--------- 435

Query: 430 STERQVPYHRPQSKIEAIQEHMAHASSKFKSEAFREDDNAMRKADEVPVNRESSVKGGST 489
                                            F   DN   K      N +S + G   
Sbjct: 436 --------------------------------GFMNGDNGSMK------NNDSGLLGS-- 495

Query: 490 VHPGSTWDASSLEQPRNTSMPDWRDN--PNNISSGTPDKGWLQPSKNLNDGWGSNTATPS 549
            H G    ASS+ +  + +   +        +S G+P+                +  T S
Sbjct: 496 -HNGGLGAASSVPRLNSVASESYGSGGAGYQLSHGSPEA-------------VRSVFTKS 537

Query: 550 YPKDNPKWQTGEESII---RRQLSGILDKEQLARKTYQSA--PEDLQLHYIDPSGAIQGP 609
              D      G ES++    +  +G L +        + A  PE+    YIDP G IQGP
Sbjct: 556 SVLD------GSESVVGSFEQAYTGKLQQPDTEVDHSEGAMPPEEFLFLYIDPQGVIQGP 537

Query: 610 FSGADIIQWFEGGYFGLDLPVRPANPPNDLPFSALGDVMPHLRSKA 644
           F G+DII WFE G+FG DL VR A+ P   PF  LG VM ++++++
Sbjct: 616 FIGSDIISWFEQGFFGTDLQVRLASAPEGTPFQDLGRVMSYIKAES 537

HSP 2 Score: 56.6 bits (135), Expect = 2.4e-07
Identity = 26/42 (61.90%), Postives = 34/42 (80.95%), Query Frame = 0

Query: 1796 DGSAKGGGKKKGKKGKKVSPSVLGFNVVSNRIMMGEIQTVED 1838
            +GS  GGGKKKGKKG+++ P++LGF V SNRI+MGEI   +D
Sbjct: 1450 EGSKGGGGKKKGKKGRQIDPALLGFKVTSNRILMGEIHRADD 1491