Spg033707 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg033707
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING-type domain-containing protein
Location	scaffold13: 39097005 .. 39102318 (+)
RNA-Seq Expression	Spg033707
Synteny	Spg033707

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAGCAAACGGGACCAACACAACGTCCACCCGGAACAAGAGTCGGGTCCCAGCCTCGCCCTCGGGCCGAGGCCGACCCCAGGCTTATGCCCGGGGCACGCCCGGTGCTCCTGGGTCGAGCCTCCGAGGTTGTAGGAGAGTCTAGTTGGACCGGACCGGATCCCGAATCGTCCTAGAACGGAGGCAAAACCCTTCCAAGTTGACTCAGGTATAAATATCATTCTTCATGTCTGGAGAAGGGTTCGAAACCTTGATTCATGACACTCTTTACTTCTTATTTGCTAACTTAAGCATCGGATTAGGTGTGGCAAGCACCACACCGGTGTGCAGTTTCTTTTAGTTTTGCAGGTCACGTCTTCTCTAGCCTCTACAAATTCACTGTTGGTGTCTGTCATGTGAAGGTCAAGTGAATCTTTTGTCCAGATTTTGACATCAACACATATACTTCTGAAAATATTTGTTCAAAACAATCAAATAAGAAAATTATATTTCCTAAAACATATAAGCATTAATCAAATATCAAAGGTAAGAAACAGTTTAAAATTTATATCGTTCCTTTTATTTCCAAAATTAATTAATTAATTAATTAATATTTCTACCTGTCTCTTAAAATATATGTTTTTAACATAACGATAAAGATACAAGACACAATACACATATAACAACCACAAACCATTTGATTTTTTTTAGTACAATAAATTGAACGCATTTGAAATTTACAAATCAGTTGGACAAAATCGAAGACCTGACCTTGGGGTATTTTTGTCCTTCTAATAATGACATAATTGATCAAATCAACAATTGGTCAATCAAGATCCAATGGTGCACACTCTTGCATTTACCTAACCTTTTTTTCTTTTTTGAAGGTTCTTACCTAACCATTGACACATGCAACTTTGAAAAATAATATTTCTTTCAAGAAATAACACTGGTATCAATTTTTATTATCAGTTTTCAATTTTATTATATTACACCTTAAATTTAGATAAATATTATAATTTTTACCATTTAAAAAAAAATTATAATATTTACATTAAATTTCAAATATTGTTACAAATAAGTTTTTGTCCCAACGATCATTAAATTATACTACAAATATATCATACAATTTTGATAGGGTTTCAAAGAAAAAAAACTATAAGTTTTACCTTAAGCTTAAAAGTTAAAATATTTTACAATTTTTACTTTGTGTTAAGTGTTTATTTGAGCGATTATTTAAAAATATTATGAAGCATATTCGATATACTATAAAACAAATATATGATACAAATTTGATAGGATTTCAACTTTACTTTCTAAAAAAAATGATAGAATTTCCAATGAAAAATAACATCAGAGAAACTAGTTTTGTAATATTTTACTTTATTTATATTATAATAAAAAGTCTTAATTTTATTCATTAGAAAAAGTAAGTTATCTACAATCTATTAATCTATTATCTTTTATTTCTCTTTTTTCTTTTTTCATTTTTAAATTTAATACATATTATATTATGTATTATTTATAAATTTAATTATTAATAATTCAGTATAAATTATGAGTATGGCTTGCAATTTTCAATATAATTGCAATATTTAGGTTCATCCTTTTTCTCATGTTTCATTTGTTTTATTGTAATGGCCATGTAATTTCAATGTCTAGATTCATCTATTTTGCCTTGTTTTATTTATATATTATAGATATATTATTTTAGTAATATATTTTAACTTATTATAATTAATTTATAATAATAATAATAAAAATAAATTACAAACTAAACTTTAATTCATACTTATCTTCTTTTCTCAAAAAAAAAAAAAAAAGAATCATACTTATCTTCTTCGGCGGGTTTAATAAGTATATTATTTTAATAATATATTTTAGTTTAAGTATTAATAATTAATTAAAAATACTTATAAAAAAATTAGTTTAAAATAATAATCGATTATAAAAGTTAATTGATGATCAACATTTTCAGAAAAGCCTATTACAATTCCTCAATTTAGGCCATTAATAGTTTAATCTTTCCCACAGTCAATTGGTATTCTCAAGTTTTGGTAAATGTGAAAGGTTCATGTGGATACTAACCATCATTAAATTATATTTTAGTTTTAAATTTTACAGAGCACGCGATATTAACTAGTAACTTTAAGATCTAATTCAATAAAACTGAACTAATCTAAACGCTAAATAAAACACTAATTACTATTCTAAAGATGAAGGGTTGAATTAGTAATTATTAAATTCAAAAAAGAAAAAATGGAATGGAATTGAAAATTGATACACGTGTCAAACTTTAAGGATAAAAATTAATTCATTACCTATATTTTTTTCCTATTGAACTAAAAAAAATCTAATATCCCTACTCTCTTTTGCAGCCGTTGATAGTTTGACTTCAATGGGAAGACGTAACCAATAATCTTATGACACGTGGGGACAAGTTGACGAGAATCACGGAGAAAGTGTGATGATGCACGCAGTGAATATTGTTTAGGGCACGGCTGCGTGAAGCTTCCATCTTCAAACGTATATTTTCAGCATTTCTGTCAGACGCGTTCGTCTTCGGTTTCAGATTCTCTTCTTCATCGATCGGAACGCGGGATCGGCCGGTTTGTTCTTCTCAATCAATAGGTCCGTGTTTTCATTTTTCCGATTCTTTTTGTAATTGTATTTTGATTTCTGAGTTTTTTTCTTTTTGTTGGATTGTTCGCATTGAATTTGATTCTGTTGGTCTCTGTTTTGGGATTTTGAAACCCTAGGATTTGAGATTTGGCTGTATATTTCCCGCTTGTGTTGTTCTGCTGGTCGATCCATTGTTAAGATTGTTGATTTTCTTTAGGTTCTCCCATTGGTTTTTGTTGGTCGAAGGACTATTTCCGACCCCGAAGATTTTTTTTTCTGTTGACTTTCGTGGATTTCTTTCTCTTATCTCAACTTTGTGGTTTAATGTGATTTAATAGGTAGGGCGTGTAGAAATGTCTAGCGGGTTTGGGGAATCGACGAGTAGGCAACCCCAAAGCCCTTCGTGTTCCGACAACAATGGTGATGCTAGTAATTTTGAGTGCAATATTTGCCTGGATTTAGCTCAAGATCCTATTGTAACCCTATGCGGTCATCTTTTTTGTTGGCCATGCCTTTATAAGTGGCTGCACATTCATTCCCATCCCCAGGAATGTCCAGTTTGTAAGGCTATCATAGAAGAGCAAAAATTGGTTCCCTTATATGGTAGGGGAAAGACATCGACTGACCCGAGATCGAAAAGTATTCCAGGGATTAATATTCCTAATCGTCCTGCGGGACAGAGACCTGAAGCAGCAGGTCCTCCCCCTGAACCAAATCCTTTTCCTCACAATGGGTTTGGATTCATGGGAGGTCTTGGAGGGTTTGCCCCAATGGCGTCGACGAGGTTTGGTAATTTTACATTGTCTGCAGCTTTTGGTGGTCTTGTCCCATCTTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTAACTTTCCTTATGGGTTTCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGATACTATCACCATCTCCGTCAAGGTCAGCAAGATTATTACCTGAAGCGGCTGTTTTTGTTTATTGGTTTCTGTGTTCTTCTTGCCCTAATCTGGCAATAGATGAAAGAGTCTATTAGGGCTTATATTGCTTCGTGGTGTTTAGTGTATTTAATCTCTAATGTAAGAATTAAAAAGAAAATCTGATGGTACATTGTCTTGATACCTCATCATTCGTCCTAATTAGTCTTACTGTATGTTGAAATGGACATCGACTTTTATATGTTTAGGCATGGTGAACATTAGTTGATGCAATTGTATTTGAAGGTTTCGATTTCTGCACATATATATATTCATTTACATGCTGACCCACCAAATGCAATCACATGGCAGAGTGAACAAAACACATGCTGTGATGGCTTTTTCTCTCTAAGATGATTTATTTGCCTAAGTGGTTTCTCTAGTGCTCTTACCTAATGCACTACTTGCAGCATGTGTTTCCTGTTTTTTCTTTGGTCGATATATCTAGGCTGATTTTGGTGAAGAAGATAGGGTGCGAATGTGTTGCTGCTGCACATGGTGTTAGTAAGTATACCAAAAACTTGGTTATTAAATAGTGTCTGGATGTGGTTCTATCGATGTCTATTGTTTCAATTAACAATTCTAGCTTGGTATTTGTTTCGTTTTCTCAATCTTGCACTAGCTTCATGTACCATGTCATTTCATTATATGATAGAAAGGGAGCAGGATCGGGATCAGGATCATCCCCTGTTGCCATGTTGGGAACTAGTAGGTTGGTGTTCTAGCTTTCAAGGTCTTTTTTGTTTAATTTATAGGGAGTCAACTAACCAAAGGGGGATCTTTTAGCTGGATGATAGTTGACCATGTTTATCAGAAGTGTGCAGAGAAGAAGTATAAAGTGCAGTGGCTTGTAGTGTTCAATTGTCTGATGTTAATATTTTTTTTCTTAAGCCTGGAGTATTATGTTAGGATGTGGCGATTGTGGGTCCAATGAAATATTTAACACCCCTTCATCTGGCAAAGGTTAGCATTTAGTGGTTTATGCTAGTTGATACGTTATTTATTTTTGTCTGTTACTTTGTTATTGTTTATCTTTTGAAGGCCAGACGATGTCAAAATTTCTCAGAATGCATTTTCTTTTCCTTTTTAAAGTTTAGCTCCTAGCTTCATTTTAGAAAATAATTTGCAAGCCTGTTTTAGAGATGTCAATTGGGGGGTGGGGGGATTGTCTCCCTATCCCCGCTCCCAAATCATCCCGTTAAATTTTGCGAGAATCGGAATGAGTTGTGTGAAAATGGGAATGGAAATGAGGAGTGCAATCCATTCCCCCACCCTTTGTCTCACAGGGTGATAGGGCCCTATCGTAGAAAGGAAGAATTGAGGTTTTTTCTTTTTTGAGTCTAGGGATTCGAACTTTAGACCTCTTGATCGAAGATACATATCAATTGTATTTGAAATTTGTAAGCTCAAGGATTAAGTAGACATCGAAGTTTATGGACTAAATTTTAAGTTACACCGTAATATTTTTTTACTATTGAATTCTCGTGTCATCTTTCACTTTTATTTTAATCTTTTAAAAACTTTAATTCCATCTTACACATGATGATTTTTTTTTTTTTCTTTTTGAAAAGAAGCTTAAGTCCTAACTCCTAACTGTTGAAGGTCTCATATTAAAAGATAAGTGGAGACCTTACAAGTTATAAGATAGATGGATCACTCCACTCAATTGTCAATTGGTTTTGAAATGGAACTCAATGTCATCTAAT

mRNA sequence

ATGTCGAGCAAACGGGACCAACACAACGTCCACCCGGAACAAGAGTCGGGTCCCAGCCTCGCCCTCGGGCCGAGGCCGACCCCAGGCTTATGCCCGGGGCACGCCCGGTGCTCCTGGGTCGAGCCTCCGAGGTGTGGCAAGCACCACACCGGTGTGCAGTTTCTTTTAGTTTTGCAGGTCACGTCTTCTCTAGCCTCTACAAATTCACTGTTGGTGTCTGTCATGGCACGGCTGCGTGAAGCTTCCATCTTCAAACGTATATTTTCAGCATTTCTGTCAGACGCGTTCGTCTTCGGTTTCAGATTCTCTTCTTCATCGATCGGAACGCGGGATCGGCCGGTAGGGCGTGTAGAAATGTCTAGCGGGTTTGGGGAATCGACGAGTAGGCAACCCCAAAGCCCTTCGTGTTCCGACAACAATGGTGATGCTAGTAATTTTGAGTGCAATATTTGCCTGGATTTAGCTCAAGATCCTATTGTAACCCTATGCGGTCATCTTTTTTGTTGGCCATGCCTTTATAAGTGGCTGCACATTCATTCCCATCCCCAGGAATGTCCAGTTTGTAAGGCTATCATAGAAGAGCAAAAATTGGTTCCCTTATATGGTAGGGGAAAGACATCGACTGACCCGAGATCGAAAAGTATTCCAGGGATTAATATTCCTAATCGTCCTGCGGGACAGAGACCTGAAGCAGCAGGTCCTCCCCCTGAACCAAATCCTTTTCCTCACAATGGGTTTGGATTCATGGGAGGTCTTGGAGGGTTTGCCCCAATGGCGTCGACGAGGTTTGGTAATTTTACATTGTCTGCAGCTTTTGGTGGTCTTGTCCCATCTTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTAACTTTCCTTATGGGTTTCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGATACTATCACCATCTCCGTCAAGGTCAGCAAGATTATTACCTGAAGCGGCTGTTTTTGTTTATTGGTTTCTGTGTTCTTCTTGCCCTAATCTGGCAATAG

Coding sequence (CDS)

ATGTCGAGCAAACGGGACCAACACAACGTCCACCCGGAACAAGAGTCGGGTCCCAGCCTCGCCCTCGGGCCGAGGCCGACCCCAGGCTTATGCCCGGGGCACGCCCGGTGCTCCTGGGTCGAGCCTCCGAGGTGTGGCAAGCACCACACCGGTGTGCAGTTTCTTTTAGTTTTGCAGGTCACGTCTTCTCTAGCCTCTACAAATTCACTGTTGGTGTCTGTCATGGCACGGCTGCGTGAAGCTTCCATCTTCAAACGTATATTTTCAGCATTTCTGTCAGACGCGTTCGTCTTCGGTTTCAGATTCTCTTCTTCATCGATCGGAACGCGGGATCGGCCGGTAGGGCGTGTAGAAATGTCTAGCGGGTTTGGGGAATCGACGAGTAGGCAACCCCAAAGCCCTTCGTGTTCCGACAACAATGGTGATGCTAGTAATTTTGAGTGCAATATTTGCCTGGATTTAGCTCAAGATCCTATTGTAACCCTATGCGGTCATCTTTTTTGTTGGCCATGCCTTTATAAGTGGCTGCACATTCATTCCCATCCCCAGGAATGTCCAGTTTGTAAGGCTATCATAGAAGAGCAAAAATTGGTTCCCTTATATGGTAGGGGAAAGACATCGACTGACCCGAGATCGAAAAGTATTCCAGGGATTAATATTCCTAATCGTCCTGCGGGACAGAGACCTGAAGCAGCAGGTCCTCCCCCTGAACCAAATCCTTTTCCTCACAATGGGTTTGGATTCATGGGAGGTCTTGGAGGGTTTGCCCCAATGGCGTCGACGAGGTTTGGTAATTTTACATTGTCTGCAGCTTTTGGTGGTCTTGTCCCATCTTTATTCAACTTTCAGATGCATGGATATGCAGATCCAAGCATGTATGGTGCAGGTGCTAACTTTCCTTATGGGTTTCCTAACATGTTTCATGGTAGCCATGGTCACGCCCATGGATACTATCACCATCTCCGTCAAGGTCAGCAAGATTATTACCTGAAGCGGCTGTTTTTGTTTATTGGTTTCTGTGTTCTTCTTGCCCTAATCTGGCAATAG

Protein sequence

MSSKRDQHNVHPEQESGPSLALGPRPTPGLCPGHARCSWVEPPRCGKHHTGVQFLLVLQVTSSLASTNSLLVSVMARLREASIFKRIFSAFLSDAFVFGFRFSSSSIGTRDRPVGRVEMSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGANFPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ

Homology

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BLAST of Spg033707 vs. NCBI nr
Match: XP_038884023.1 (E3 ubiquitin ligase rnf-5 [Benincasa hispida])

HSP 1 Score: 481.9 bits (1239), Expect = 4.7e-132
Identity = 227/230 (98.70%), Postives = 228/230 (99.13%), Query Frame = 0


Query: 119 MSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 178
           M+SGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 179 HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 238
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 239 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 298
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 299 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ 349
           FPYGFPNMFHGSHGHAHGYYH LRQGQQDYYLKRL LFIGFCVLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHQLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Spg033707 vs. NCBI nr
Match: KAG6598412.1 (E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 480.3 bits (1235), Expect = 1.4e-131
Identity = 229/242 (94.63%), Postives = 233/242 (96.28%), Query Frame = 0


Query: 107 IGTRDRPVGRVEMSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHL 166
           IGT+   VG  +M+SGFGESTSRQPQ PS SDNNGDASNFECNICLDLAQDPIVTLCGHL
Sbjct: 8   IGTQ---VGCADMASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHL 67

Query: 167 FCWPCLYKWLHIHSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG 226
           FCWPCLYKWLHIHSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG
Sbjct: 68  FCWPCLYKWLHIHSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG 127

Query: 227 QRPEAAGPPPEPNPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHG 286
           QRPEAAGPPPEPNPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHG
Sbjct: 128 QRPEAAGPPPEPNPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHG 187

Query: 287 YADPSMYGAGANFPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALI 346
           YADPSMYGAGAN PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRL LFIGFCVLLALI
Sbjct: 188 YADPSMYGAGANVPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALI 246

Query: 347 WQ 349
           WQ
Sbjct: 248 WQ 246

[Close]

BLAST of Spg033707 vs. NCBI nr
Match: XP_023547002.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >XP_023547003.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >KAG7029364.1 RING finger protein 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 475.7 bits (1223), Expect = 3.4e-130
Identity = 224/230 (97.39%), Postives = 226/230 (98.26%), Query Frame = 0


Query: 119 MSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 178
           M+SGFGESTSRQPQ PS SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 179 HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 238
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 239 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 298
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 299 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ 349
            PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRL LFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Spg033707 vs. NCBI nr
Match: XP_022996954.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita maxima] >XP_022996955.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita maxima])

HSP 1 Score: 474.9 bits (1221), Expect = 5.8e-130
Identity = 224/230 (97.39%), Postives = 225/230 (97.83%), Query Frame = 0


Query: 119 MSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 178
           MS GFGESTSRQPQ PS SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MSRGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 179 HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 238
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 239 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 298
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 299 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ 349
            PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRL LFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Spg033707 vs. NCBI nr
Match: XP_022962412.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022962413.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata])

HSP 1 Score: 474.6 bits (1220), Expect = 7.6e-130
Identity = 223/230 (96.96%), Postives = 226/230 (98.26%), Query Frame = 0


Query: 119 MSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 178
           M+SGFGESTSRQPQ PS SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 179 HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 238
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRP+GQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPSGQRPEAAGPPPEP 120

Query: 239 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 298
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 299 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ 349
            PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRL LFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Spg033707 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-31
Identity = 90/242 (37.19%), Postives = 121/242 (50.00%), Query Frame = 0


Query: 127 TSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECP 186
           T    + P+ S N  +++ FECNICLD A+D +V+LCGHLFCWPCL +WL    + Q CP
Sbjct: 5   TKAPSEEPTSSSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNNQVCP 64

Query: 187 VCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGF 246
           VCK+ I+  K+VP+YGRG  S+DPR K      +P RP GQR E     P P  F    +
Sbjct: 65  VCKSAIDGNKVVPIYGRGGDSSDPRKK------VPPRPKGQRSE-----PPPQSFAGFNW 124

Query: 247 GFMGG-LGGFAPMASTRFGNFTLSAAF---------GGLVPSLFNFQMHGYADPSMYGAG 306
           G  GG +GG  P     FG  T++  F          G+ P  F     G  +     AG
Sbjct: 125 GGDGGMMGGGGPNVHFSFGIGTVNGLFPLMFMLPFIQGIFPLSFVASFFGNGNQGAAAAG 184

Query: 307 ANFPYGFPNMFHGSHGHAHGYYH-----------HLRQGQQDYYLKRLFLFIGFCVLLAL 348
                G      G+H H HG+ H             R  Q++ YL  +F +IGF +L  L
Sbjct: 185 GGNGGGNDGN-DGTHAHTHGHTHGPRGHGESAAPGSRMAQEEEYLSNIFKYIGFFMLFWL 234

[Close]

BLAST of Spg033707 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 4.7e-26
Identity = 69/144 (47.92%), Postives = 80/144 (55.56%), Query Frame = 0


Query: 113 PVGRVEMSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCL 172
           P       S     TS    S    DN    + FECNICLD A+D +++LCGHLFCWPCL
Sbjct: 6   PTTSASTKSSSTGGTSGSSSSNGAGDNTNQDNTFECNICLDTAKDAVISLCGHLFCWPCL 65

Query: 173 YKWLHIHSHPQECPVCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEA 232
           ++WL    + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE 
Sbjct: 66  HQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE- 125

Query: 233 AGPPPEPNPFPHNGFGFMGGLGGF 256
               PE N     GFGF  G GGF
Sbjct: 126 ----PE-NRGGFQGFGF--GDGGF 135

[Close]

BLAST of Spg033707 vs. ExPASy Swiss-Prot
Match: Q96GF1 (E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.4e-25
Identity = 71/139 (51.08%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 118 EMSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLH 177
           E SS  G S S    S    ++ G  S FECNICLD A+D +++LCGHLFCWPCL++WL 
Sbjct: 13  ENSSAGGPSGS----SNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLE 72

Query: 178 IHSHPQECPVCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAAGPPP 237
              + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE     P
Sbjct: 73  TRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-----P 132

Query: 238 EPNPFPHNGFGFMGGLGGF 256
           E N     GFGF  G GGF
Sbjct: 133 E-NRGGFQGFGF--GDGGF 133

[Close]

BLAST of Spg033707 vs. ExPASy Swiss-Prot
Match: Q5RFK9 (E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 1.4e-25
Identity = 71/139 (51.08%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 118 EMSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLH 177
           E SS  G S S    S    ++ G  S FECNICLD A+D +++LCGHLFCWPCL++WL 
Sbjct: 13  ENSSAGGPSGS----SNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLE 72

Query: 178 IHSHPQECPVCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAAGPPP 237
              + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE     P
Sbjct: 73  TRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-----P 132

Query: 238 EPNPFPHNGFGFMGGLGGF 256
           E N     GFGF  G GGF
Sbjct: 133 E-NRGGFQGFGF--GDGGF 133

[Close]

BLAST of Spg033707 vs. ExPASy Swiss-Prot
Match: Q568Y3 (E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.8e-25
Identity = 71/139 (51.08%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 118 EMSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLH 177
           E SS  G S S    S    ++ G  S FECNICLD A+D +++LCGHLFCWPCL++WL 
Sbjct: 13  ENSSAGGPSGS----SNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLE 72

Query: 178 IHSHPQECPVCKAIIEEQKLVPLYGRGKT-STDPRSKSIPGINIPNRPAGQRPEAAGPPP 237
              + Q CPVCKA I   K++PLYGRG T   DPR K+      P RP GQRPE     P
Sbjct: 73  TRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKT------PPRPQGQRPE-----P 132

Query: 238 EPNPFPHNGFGFMGGLGGF 256
           E N     GFGF  G GGF
Sbjct: 133 E-NRGGFQGFGF--GDGGF 133

[Close]

BLAST of Spg033707 vs. ExPASy TrEMBL
Match: A0A6J1KA44 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111492032 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.8e-130
Identity = 224/230 (97.39%), Postives = 225/230 (97.83%), Query Frame = 0


Query: 119 MSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 178
           MS GFGESTSRQPQ PS SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MSRGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 179 HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 238
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 239 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 298
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 299 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ 349
            PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRL LFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Spg033707 vs. ExPASy TrEMBL
Match: A0A6J1HCN0 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC111462860 PE=4 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 3.7e-130
Identity = 223/230 (96.96%), Postives = 226/230 (98.26%), Query Frame = 0


Query: 119 MSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 178
           M+SGFGESTSRQPQ PS SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQGPSYSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 179 HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 238
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRP+GQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKALIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPSGQRPEAAGPPPEP 120

Query: 239 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 298
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 299 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ 349
            PYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRL LFIGFCVLLALIWQ
Sbjct: 181 VPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLLFIGFCVLLALIWQ 230

[Close]

BLAST of Spg033707 vs. ExPASy TrEMBL
Match: A0A6J1CT42 (E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC111014071 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 6.2e-130
Identity = 223/230 (96.96%), Postives = 225/230 (97.83%), Query Frame = 0


Query: 119 MSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 178
           M+SGFGESTSRQPQSPSCSDNN DASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNSDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 179 HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 238
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 239 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 298
           N FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGA 
Sbjct: 121 NHFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAG 180

Query: 299 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ 349
           FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRL + IGFCVLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLLMLIGFCVLLALIWQ 230

[Close]

BLAST of Spg033707 vs. ExPASy TrEMBL
Match: A0A0A0LLD2 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360850 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 8.2e-130
Identity = 224/230 (97.39%), Postives = 224/230 (97.39%), Query Frame = 0


Query: 119 MSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 178
           M SGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MESGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 179 HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 238
           HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 120

Query: 239 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 298
           NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 299 FPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ 349
           FPYGFPNMFHGSHGHAHGYYH LRQGQQDYYLKRL   IGF VLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHAHGYYHQLRQGQQDYYLKRLLFLIGFFVLLALIWQ 230

[Close]

BLAST of Spg033707 vs. ExPASy TrEMBL
Match: A0A6J1GGW7 (RING finger protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111454080 PE=4 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 1.1e-129
Identity = 224/234 (95.73%), Postives = 227/234 (97.01%), Query Frame = 0


Query: 119 MSSGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 178
           M+SGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI
Sbjct: 1   MASGFGESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHI 60

Query: 179 HSHPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP 238
           HSHPQECPVCKA+IEEQKLVPLYGRGKTSTDPRSKSIPG+NIPNRPAGQRPEAAGPPPEP
Sbjct: 61  HSHPQECPVCKAMIEEQKLVPLYGRGKTSTDPRSKSIPGVNIPNRPAGQRPEAAGPPPEP 120

Query: 239 NPFPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 298
           NPF HNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN
Sbjct: 121 NPFAHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN 180

Query: 299 FPYGFPNMFHGSHG----HAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALIWQ 349
           FPYGFPNMFHGSHG    HAHGYYHHLRQGQQDYYLKRL  FIGFCVLLALIWQ
Sbjct: 181 FPYGFPNMFHGSHGHGHAHAHGYYHHLRQGQQDYYLKRLLFFIGFCVLLALIWQ 234

[Close]

BLAST of Spg033707 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 5.0e-79
Identity = 146/220 (66.36%), Postives = 170/220 (77.27%), Query Frame = 0


Query: 134 PSCSD----NNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCK 193
           PSCS     NN D+SNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLH+HS  ++CPVCK
Sbjct: 5   PSCSSGNDTNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKDCPVCK 64

Query: 194 AIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGFGFM 253
           A+IEE +LVPLYGRGK+S DPRSKSIPG+ +PNRP+GQRPE A PP   + F H+  GF 
Sbjct: 65  AVIEEDRLVPLYGRGKSSADPRSKSIPGLEVPNRPSGQRPETAQPPDPNHGFAHH-HGFG 124

Query: 254 GGLGGF-APMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGAN--FPYGFPNM 313
           G +GGF APMAS RFGN TLSAAFGGL+PSLFN   HG+ D +MYGA A+  FP+GF N 
Sbjct: 125 GFMGGFAAPMASARFGNVTLSAAFGGLIPSLFNLHFHGFPDAAMYGAAASGGFPHGFSNP 184

Query: 314 FHGSHGHAHGYYHHL-RQGQQDYYLKRLFLFIGFCVLLAL 346
           FHG H H H Y  H  RQGQQD++L+ L L +   V+ +L
Sbjct: 185 FHGGHSHMHSYQRHTGRQGQQDHHLRILLLIVFVVVVFSL 223

[Close]

BLAST of Spg033707 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 2.6e-67
Identity = 130/230 (56.52%), Postives = 160/230 (69.57%), Query Frame = 0


Query: 124 GESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQ 183
           GES++    S S +D N    +FECNIC +LAQDPIVTLCGHLFCWPCLY+WLH HSH Q
Sbjct: 4   GESSTSTSYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLHHHSHSQ 63

Query: 184 ECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEP---NP 243
           ECPVCKA++++ KLVPLYGRGK  TDPRSK  PG+ IPNRP GQRPE A PPP+P   + 
Sbjct: 64  ECPVCKAVVQDDKLVPLYGRGKNQTDPRSKRYPGLRIPNRPTGQRPETAAPPPQPEAASN 123

Query: 244 FPHNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMYGAGANFP 303
           F + G G MGG+     MA+TRFGNF  S  FGGL+PSLFNFQ HG+ D ++YG+   +P
Sbjct: 124 FFNYGIGLMGGI--MPMMATTRFGNF--SMGFGGLLPSLFNFQFHGFHDATLYGSTPGYP 183

Query: 304 Y-GFPNMFHGSHGHAHGYYHHLRQG--QQDYYLKRLFLFIGFCVLLALIW 348
           Y G+ N F G      G    + +G  Q D +LK +  F+G CV++ LIW
Sbjct: 184 YGGYHNGFRGV--PPRGQERPMARGGNQSDAFLKNILFFVGICVVIFLIW 227

[Close]

BLAST of Spg033707 vs. TAIR 10
Match: AT1G74990.1 (RING/U-box superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 2.5e-38
Identity = 76/133 (57.14%), Postives = 91/133 (68.42%), Query Frame = 0


Query: 137 SDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHSHPQECPVCKAIIEEQK 196
           ++ +  ++NF CNICL+LA++PIVTLCGHLFCWPCLYKWLH HS    CPVCKA+++E  
Sbjct: 8   NEEDDASNNFGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKSNHCPVCKALVKEDT 67

Query: 197 LVPLYGRGKTSTDPRSKSIPGINIPNRPAGQRPEAAGPPPEPNPFPHNGFGFMGGLGGFA 256
           LVPLYG GK S+DPRSK   G+ +PNRPA  R E A P  E     H+G  F GG   FA
Sbjct: 68  LVPLYGMGKPSSDPRSKLNSGVTVPNRPAATRTETARPRLEQR---HHGSSFFGGHSSFA 127

Query: 257 PM-ASTRFGNFTL 269
            M    RF NF L
Sbjct: 128 AMPTGLRFSNFLL 137

[Close]

BLAST of Spg033707 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-20
Identity = 72/233 (30.90%), Postives = 111/233 (47.64%), Query Frame = 0


Query: 125 ESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHS---- 184
           +S   +P + S   ++ D SNF+CNICLD  Q+P+VTLCGHLFCWPC++KWL + S    
Sbjct: 25  KSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTS 84

Query: 185 ----HPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG--QRPEAAGP 244
                 ++CPVCK+ +    LVPLYGRG+ +T    K+    ++P RP G   R E    
Sbjct: 85  DEYQRHRQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKN----SVPKRPVGPVYRLEMPNS 144

Query: 245 PPEPNPFP-HNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMY 304
           P             F     G+ P++     N   S ++  ++  +    +       ++
Sbjct: 145 PYASTDLRLSQRVHFNSPQEGYYPVSGVMSSN---SLSYSAVLDPVM-VMVGEMVATRLF 204

Query: 305 GAGANFPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALI 347
           G      + +P+ +  +     G     R  Q D  L R+F F   CV+L L+
Sbjct: 205 GTRVMDRFAYPDTY--NLAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLL 247

[Close]

BLAST of Spg033707 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 97.8 bits (242), Expect = 1.8e-20
Identity = 72/233 (30.90%), Postives = 111/233 (47.64%), Query Frame = 0


Query: 125 ESTSRQPQSPSCSDNNGDASNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHIHS---- 184
           +S   +P + S   ++ D SNF+CNICLD  Q+P+VTLCGHLFCWPC++KWL + S    
Sbjct: 25  KSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTS 84

Query: 185 ----HPQECPVCKAIIEEQKLVPLYGRGKTSTDPRSKSIPGINIPNRPAG--QRPEAAGP 244
                 ++CPVCK+ +    LVPLYGRG+ +T    K+    ++P RP G   R E    
Sbjct: 85  DEYQRHRQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKN----SVPKRPVGPVYRLEMPNS 144

Query: 245 PPEPNPFP-HNGFGFMGGLGGFAPMASTRFGNFTLSAAFGGLVPSLFNFQMHGYADPSMY 304
           P             F     G+ P++     N   S ++  ++  +    +       ++
Sbjct: 145 PYASTDLRLSQRVHFNSPQEGYYPVSGVMSSN---SLSYSAVLDPVM-VMVGEMVATRLF 204

Query: 305 GAGANFPYGFPNMFHGSHGHAHGYYHHLRQGQQDYYLKRLFLFIGFCVLLALI 347
           G      + +P+ +  +     G     R  Q D  L R+F F   CV+L L+
Sbjct: 205 GTRVMDRFAYPDTY--NLAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLL 247

The following BLAST results are available for this feature:

BLAST of Spg033707 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884023.1	4.7e-132	98.70	E3 ubiquitin ligase rnf-5 [Benincasa hispida]	[more]
KAG6598412.1	1.4e-131	94.63	E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023547002.1	3.4e-130	97.39	E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >XP_0235470...	[more]
XP_022996954.1	5.8e-130	97.39	E3 ubiquitin-protein ligase RNF185-like [Cucurbita maxima] >XP_022996955.1 E3 ub...	[more]
XP_022962412.1	7.6e-130	96.96	E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022962413.1 E3 ...	[more]

BLAST of Spg033707 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q09463	1.7e-31	37.19	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]
Q5ZIR9	4.7e-26	47.92	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1	[more]
Q96GF1	1.4e-25	51.08	E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1	[more]
Q5RFK9	1.4e-25	51.08	E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1	[more]
Q568Y3	1.8e-25	51.08	E3 ubiquitin-protein ligase RNF185 OS=Rattus norvegicus OX=10116 GN=Rnf185 PE=2 ...	[more]

BLAST of Spg033707 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KA44	2.8e-130	97.39	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111492...	[more]
A0A6J1HCN0	3.7e-130	96.96	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1CT42	6.2e-130	96.96	E3 ubiquitin-protein ligase RNF185 OS=Momordica charantia OX=3673 GN=LOC11101407...	[more]
A0A0A0LLD2	8.2e-130	97.39	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360850 P...	[more]
A0A6J1GGW7	1.1e-129	95.73	RING finger protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111454080 PE=4 SV=1	[more]

BLAST of Spg033707 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G19310.1	5.0e-79	66.36	RING/U-box superfamily protein	[more]
AT2G23780.1	2.6e-67	56.52	RING/U-box superfamily protein	[more]
AT1G74990.1	2.5e-38	57.14	RING/U-box superfamily protein	[more]
AT4G03510.1	1.8e-20	30.90	RING membrane-anchor 1	[more]
AT4G03510.2	1.8e-20	30.90	RING membrane-anchor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 148..188 e-value: 6.4E-8 score: 42.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 148..189 score: 13.256433
None	No IPR available	PFAM	PF13923	zf-C3HC4_2	coord: 147..188 e-value: 3.5E-8 score: 33.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 113..138
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 202..240
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..25
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 121..138
None	No IPR available	PANTHER	PTHR12313:SF39	OS12G0636000 PROTEIN	coord: 120..348
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 146..190 e-value: 2.965E-30 score: 107.39
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 144..201
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 128..202 e-value: 5.5E-17 score: 62.9
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 120..348
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 163..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg033707.1	Spg033707.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity