Spg033644 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg033644
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferase
Locationscaffold13: 38838124 .. 38839659 (+)
RNA-Seq ExpressionSpg033644
SyntenySpg033644
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGATAAAGAGAATGGCTTGCCTGATGGCCCAGGGTGGTCTGAGCTAAAGCTTCCAGATATGTTGTTGGCCGACAGTGTACGTGAGGTCCATGCTCGAATTGAGGCTGAATGGGATTACCTCCAACGCTCGGCTTGTCAAATGGCAGCAGGAAGAGCGCTTTGGAAGCATGTGATCCATGATCCACTAGCGGAGTTACTTGCTGGAGAGGCATGCCTGAGAAACCTTCATGAGAAGATAAAGAAAGATCGAAGCAACAATGCAAGAGAGATTTCTGGAGTGATTCTTGCTGTTCGTACCCTCTGGTTTGATTCAAAAATTGAAGGTGCTCTAGCATCCTTCAATGGCAGAGATTCACAGGTCGTTCTCCTTGGTGCAGGTTGGTATTTCAACTCCCAGACGCTTGTGTATTTTAGCTAAAATTCATATTGCGTTAAGAGGTCCCAAACATAGCAGCAGATGGTTAGAAGATAGCAACAGCTAAAATTGCACAAATAACCAATTTGTTTTTCTTTTCCAGCAAAACGAGTCCCAATCCAGGTTGTTACTAGGCATGTTGGAATTGCAAAATAAGATAGCAGTGAAATTAGAATCTCACACACTGATATCTAAAAAATGTGCATACTCCTTTTGCTTTTTTTCTTTTTCTCCTCTCTCTATGTAGTTGGAGTGGGGGGAGGGGGATAAAACACTAGGTCAGGATAAATGAACACTATAACCTATTTAGTGACTAAAAAATGAGGCCATCAAAACAATCAAAATGGAAACTGTATGCTTAGTCAAAGAGAATTACTTGCAGTTGCATCTGAGACATTAGAGTTGGGAGAAGTGTATTAGACTTCAATTTAAGTTTTATCTGTCACTTCTATAAGTTCAAATAACAGAGATCTCCTTTACAATGTAGGAATGGATACGAGGGCTTACAGATTAAGTTGCTTGAAGCAAAGTGATGTTTTTGAAGTTGATTTCCCTGAATTGTTGCAAGTGAAAGCCACCCTTCTAAAGACAGCAATAGAATCTCCATACGGGCGCCATCATCAACAAATGGCAAAATCGTTAAACAGAGTAGCAGCTGACATCAGAAGTAATGACTGGCTTGAAAAGCTTCAATCAGCTGGCTTTGCACCAGAGAAGAGTACAGTGTGGGTTCTAGAAGGATTGCTCTACTATCTTACCCACTCCCAGGCGATGCAAGTATTGGAGACAATAGCTGACAAATGCACCATAACCCACACAGTCCTCTTAGCAGATTTTATGAACAAACCCTCAACCACTCTTTCAAATTCTGTCTTCCAATTCTACTGCGATTGGCCTGATCATCTACTTCCATCCCTTGGATTTTCCAACACTAAACTATCACAGATTGGAGACCCAGATGCTCACTTTGGGCTTATGCACGACCCATTAAATCTCTTCAATAAAATCCGCAGTCTACCTAGGTCATTGCAGACTCACCCTGATGATGGAAAACCCTGCTGCCGCTTGTATTTGGTACAGGCTTCTGGTTCACCTCAGCTACCACCAGTTGCATAA

mRNA sequence

ATGACTGATAAAGAGAATGGCTTGCCTGATGGCCCAGGGTGGTCTGAGCTAAAGCTTCCAGATATGTTGTTGGCCGACAGTGTACGTGAGGTCCATGCTCGAATTGAGGCTGAATGGGATTACCTCCAACGCTCGGCTTGTCAAATGGCAGCAGGAAGAGCGCTTTGGAAGCATGTGATCCATGATCCACTAGCGGAGTTACTTGCTGGAGAGGCATGCCTGAGAAACCTTCATGAGAAGATAAAGAAAGATCGAAGCAACAATGCAAGAGAGATTTCTGGAGTGATTCTTGCTGTTCGTACCCTCTGGTTTGATTCAAAAATTGAAGGTGCTCTAGCATCCTTCAATGGCAGAGATTCACAGGTCGTTCTCCTTGGTGCAGGAATGGATACGAGGGCTTACAGATTAAGTTGCTTGAAGCAAAGTGATGTTTTTGAAGTTGATTTCCCTGAATTGTTGCAAGTGAAAGCCACCCTTCTAAAGACAGCAATAGAATCTCCATACGGGCGCCATCATCAACAAATGGCAAAATCGTTAAACAGAGTAGCAGCTGACATCAGAAGTAATGACTGGCTTGAAAAGCTTCAATCAGCTGGCTTTGCACCAGAGAAGAGTACAGTGTGGGTTCTAGAAGGATTGCTCTACTATCTTACCCACTCCCAGGCGATGCAAGTATTGGAGACAATAGCTGACAAATGCACCATAACCCACACAGTCCTCTTAGCAGATTTTATGAACAAACCCTCAACCACTCTTTCAAATTCTGTCTTCCAATTCTACTGCGATTGGCCTGATCATCTACTTCCATCCCTTGGATTTTCCAACACTAAACTATCACAGATTGGAGACCCAGATGCTCACTTTGGGCTTATGCACGACCCATTAAATCTCTTCAATAAAATCCGCAGTCTACCTAGGTCATTGCAGACTCACCCTGATGATGGAAAACCCTGCTGCCGCTTGTATTTGGTACAGGCTTCTGGTTCACCTCAGCTACCACCAGTTGCATAA

Coding sequence (CDS)

ATGACTGATAAAGAGAATGGCTTGCCTGATGGCCCAGGGTGGTCTGAGCTAAAGCTTCCAGATATGTTGTTGGCCGACAGTGTACGTGAGGTCCATGCTCGAATTGAGGCTGAATGGGATTACCTCCAACGCTCGGCTTGTCAAATGGCAGCAGGAAGAGCGCTTTGGAAGCATGTGATCCATGATCCACTAGCGGAGTTACTTGCTGGAGAGGCATGCCTGAGAAACCTTCATGAGAAGATAAAGAAAGATCGAAGCAACAATGCAAGAGAGATTTCTGGAGTGATTCTTGCTGTTCGTACCCTCTGGTTTGATTCAAAAATTGAAGGTGCTCTAGCATCCTTCAATGGCAGAGATTCACAGGTCGTTCTCCTTGGTGCAGGAATGGATACGAGGGCTTACAGATTAAGTTGCTTGAAGCAAAGTGATGTTTTTGAAGTTGATTTCCCTGAATTGTTGCAAGTGAAAGCCACCCTTCTAAAGACAGCAATAGAATCTCCATACGGGCGCCATCATCAACAAATGGCAAAATCGTTAAACAGAGTAGCAGCTGACATCAGAAGTAATGACTGGCTTGAAAAGCTTCAATCAGCTGGCTTTGCACCAGAGAAGAGTACAGTGTGGGTTCTAGAAGGATTGCTCTACTATCTTACCCACTCCCAGGCGATGCAAGTATTGGAGACAATAGCTGACAAATGCACCATAACCCACACAGTCCTCTTAGCAGATTTTATGAACAAACCCTCAACCACTCTTTCAAATTCTGTCTTCCAATTCTACTGCGATTGGCCTGATCATCTACTTCCATCCCTTGGATTTTCCAACACTAAACTATCACAGATTGGAGACCCAGATGCTCACTTTGGGCTTATGCACGACCCATTAAATCTCTTCAATAAAATCCGCAGTCTACCTAGGTCATTGCAGACTCACCCTGATGATGGAAAACCCTGCTGCCGCTTGTATTTGGTACAGGCTTCTGGTTCACCTCAGCTACCACCAGTTGCATAA

Protein sequence

MTDKENGLPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVIHDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDSQVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPYGRHHQQMAKSLNRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTVLLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFNKIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPPVA
Homology
BLAST of Spg033644 vs. NCBI nr
Match: XP_023537171.1 (uncharacterized protein LOC111798329 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 648.7 bits (1672), Expect = 2.8e-182
Identity = 313/336 (93.15%), Postives = 322/336 (95.83%), Query Frame = 0

Query: 1   MTDKENGLPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60
           MT+KENGLPD PGW ELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLPDDPGWFELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDS 120
           HDPLA+LLAGEACLRNLHEKIKKDRSNNARE+SGVILAVRTLWFDSKIEGAL SFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPY-GRHHQQMAKSL 180
           QVVLLGAGMDTRAYRLSCLK+SDVFEVDFPELLQ KA L+K AIESPY   HHQQ+AKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180

Query: 181 NRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTV 240
           NRVAADIRS+DWLEKLQSAGFAPEK+TVWVLEGLLYY THSQAMQVLETIADKC ITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVF FYCDWPDHLLPSLGFS TKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSTTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPPV 336
           KIRSLPRSLQTHPDDGKPCCRLYLVQASGSP LPP+
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPI 336

BLAST of Spg033644 vs. NCBI nr
Match: KAG7020511.1 (hypothetical protein SDJN02_17196 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 645.6 bits (1664), Expect = 2.4e-181
Identity = 311/336 (92.56%), Postives = 323/336 (96.13%), Query Frame = 0

Query: 1   MTDKENGLPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60
           MT+KENGLPD PGW ELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLPDDPGWFELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDS 120
           HDPLA+LLAGEACLRNLHEKIKKDRSNNARE+SGVILAVRTLWFDSKIEGAL SFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPY-GRHHQQMAKSL 180
           QVVLLGAGMDTRAYRLSCLK+SDVFEVDFPELLQ KA L+K AIESP+   HHQQ+AKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPHEHHHHQQIAKSL 180

Query: 181 NRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTV 240
           NRVAADIRS+DWLEKLQSAGFAPEK+TVWVLEGLLYY THSQAMQVLETIADKC ITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVF FYCDWP+HLLPSLGFS+TKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPEHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPPV 336
           KIRSLPRSLQTHPDDGKPCCRLYLVQASGSP LPP+
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPI 336

BLAST of Spg033644 vs. NCBI nr
Match: XP_022951671.1 (uncharacterized protein LOC111454414 [Cucurbita moschata])

HSP 1 Score: 645.2 bits (1663), Expect = 3.1e-181
Identity = 311/336 (92.56%), Postives = 322/336 (95.83%), Query Frame = 0

Query: 1   MTDKENGLPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60
           MT+KENGLPD PGW ELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLPDDPGWFELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDS 120
           HDPLA+LLAGEACLRNLHEKIKKDRSNNARE+SGVILAVRTLWFDSKIEGAL SFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPY-GRHHQQMAKSL 180
           QVVLLGAGMDTRAYRLSCLK+SDVFEVDFPELLQ KA L+K AIESPY   HHQQ+AKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180

Query: 181 NRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTV 240
           NRVAADIRS+DWLEKLQSAGFAPEK+TVWVLEGLLYY THSQAMQVLETIADKC ITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVF FYCDWP+HLLPSLGFS+TKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPEHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPPV 336
           KIRSLPRSLQTHPDDGKPCCRLYLVQASG P LPP+
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGLPLLPPI 336

BLAST of Spg033644 vs. NCBI nr
Match: XP_022144259.1 (uncharacterized protein LOC111013987 [Momordica charantia] >XP_022144260.1 uncharacterized protein LOC111013987 [Momordica charantia] >XP_022144261.1 uncharacterized protein LOC111013987 [Momordica charantia])

HSP 1 Score: 645.2 bits (1663), Expect = 3.1e-181
Identity = 311/337 (92.28%), Postives = 323/337 (95.85%), Query Frame = 0

Query: 1   MTDKENG--LPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKH 60
           MTDKENG  LP+ PGWSELKLPDMLLA+SVRE HARIEAEWDYLQRSACQMAAGRALW H
Sbjct: 1   MTDKENGLRLPEDPGWSELKLPDMLLANSVREAHARIEAEWDYLQRSACQMAAGRALWSH 60

Query: 61  VIHDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGR 120
           VIHDPLA LLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGAL+SFNGR
Sbjct: 61  VIHDPLAGLLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALSSFNGR 120

Query: 121 DSQVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPYGRHHQQMAKS 180
           DSQVVLLGAGMDTRAYRLSCLK+SDVFEVDFPE+LQVK  LLKTAIESPYG HHQQMAKS
Sbjct: 121 DSQVVLLGAGMDTRAYRLSCLKESDVFEVDFPEVLQVKTALLKTAIESPYGHHHQQMAKS 180

Query: 181 LNRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHT 240
           LNR+AADIR NDWLEKLQSAGFA EK+TVWVLEGLLYYLTHSQAMQVLETIADKCTIT T
Sbjct: 181 LNRIAADIRGNDWLEKLQSAGFASEKNTVWVLEGLLYYLTHSQAMQVLETIADKCTITPT 240

Query: 241 VLLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLF 300
           VLLADFMNKPSTTLSNS+F FYCDWPDHLLPSLGFSNTKLSQIGDPDA+FGL+HDPLNLF
Sbjct: 241 VLLADFMNKPSTTLSNSIFHFYCDWPDHLLPSLGFSNTKLSQIGDPDANFGLVHDPLNLF 300

Query: 301 NKIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPPV 336
           NKIRSLPRS+QTHPDDGKPCCRLYLVQASGSP LPP+
Sbjct: 301 NKIRSLPRSMQTHPDDGKPCCRLYLVQASGSPHLPPL 337

BLAST of Spg033644 vs. NCBI nr
Match: KAG7029387.1 (hypothetical protein SDJN02_07726, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 638.6 bits (1646), Expect = 2.9e-179
Identity = 307/334 (91.92%), Postives = 317/334 (94.91%), Query Frame = 0

Query: 1   MTDKENGLPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60
           MTDKENG PD PGWSELKLPD+LL DSVRE+HARIEAEWDYLQRSACQMAAGRALWKH+I
Sbjct: 1   MTDKENGWPDNPGWSELKLPDLLLVDSVREIHARIEAEWDYLQRSACQMAAGRALWKHMI 60

Query: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDS 120
           HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGAL S NG+DS
Sbjct: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALTSINGKDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPYGRHHQQMAKSLN 180
           QVVLLGAGMDTRAYRLSCLK+SDVFEVDF ELLQ KATLLK AIE P G HHQQ AKSLN
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFSELLQAKATLLKAAIEPPNGHHHQQKAKSLN 180

Query: 181 RVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTVL 240
           RVAADIRSNDWLEKL++AGFA EK+TVWVLEGLLYYLTHSQA+QVLETIAD+CTITHTVL
Sbjct: 181 RVAADIRSNDWLEKLKTAGFASEKNTVWVLEGLLYYLTHSQALQVLETIADQCTITHTVL 240

Query: 241 LADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFNK 300
           LADFMNKPSTTLSNSVF FYCDWPDHLLPSLGFSNT LSQIGD DAHFGLMHDPLNLFNK
Sbjct: 241 LADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSNTTLSQIGDMDAHFGLMHDPLNLFNK 300

Query: 301 IRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPP 335
           IRSLPRSLQTHPDDGKPCCRLYLVQASGSPQL P
Sbjct: 301 IRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLQP 334

BLAST of Spg033644 vs. ExPASy Swiss-Prot
Match: B2HD96 (Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_2791 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=MMAR_2791 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 2.1e-15
Identity = 58/174 (33.33%), Postives = 83/174 (47.70%), Query Frame = 0

Query: 96  ILAVRTLWFDSKIEGALASFNGRDSQVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQV 155
           +  V+T +FD     A A+      Q V++ AG+D+RAYRL     + VFE+D P++L+ 
Sbjct: 80  LYGVQTRFFDDFFRSAAAA---GTKQAVIVAAGLDSRAYRLEWPSGTTVFEIDLPQVLEF 139

Query: 156 KATLLKTAIESPYGRHHQQMAKSLNRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLY 215
           KA +L+        RH  +     N VAAD+R+ DW   L +AGF P + + W +EGLL 
Sbjct: 140 KARVLQ--------RHGVEPKARRNEVAADLRT-DWPATLHAAGFEPGQPSAWSVEGLLP 199

Query: 216 YLTHSQAMQVLETIADKCT-------------ITHTVLLADFMNKPSTTLSNSV 257
           YLT      +   I D C              + H  L+A     P   LS  V
Sbjct: 200 YLTADAQDALFARIDDLCAPGSRIATGALGSRLDHDQLVALEQTHPGVNLSGDV 241

BLAST of Spg033644 vs. ExPASy Swiss-Prot
Match: A8LHA4 (Putative S-adenosyl-L-methionine-dependent methyltransferase Franean1_4929 OS=Frankia sp. (strain EAN1pec) OX=298653 GN=Franean1_4929 PE=3 SV=2)

HSP 1 Score: 84.3 bits (207), Expect = 2.8e-15
Identity = 49/139 (35.25%), Postives = 77/139 (55.40%), Query Frame = 0

Query: 91  EISGVILAVRTLWFDSKIEGALASFNGRDSQVVLLGAGMDTRAYRLSCLKQSDVFEVDFP 150
           E+    LAVRT +FD ++   LA+      QVVLL AG+D+RA+RL     + +FEVD P
Sbjct: 82  EMMNAYLAVRTRFFDDEL---LAAAEAGVRQVVLLAAGLDSRAFRLPWPAGTRLFEVDRP 141

Query: 151 ELLQVKATLLKTAIESPYGRHHQQMAKSLNRVAADIRSNDWLEKLQSAGFAPEKSTVWVL 210
           ++L  K  +L      P    H         V+AD+ + DW +++  AGF P + T W+ 
Sbjct: 142 DILAFKEQVLAAGETGPRCERH--------AVSADL-TEDWADEILDAGFRPAEPTAWLA 201

Query: 211 EGLLYYLTHSQAMQVLETI 230
           EG++ YL+  +A ++L  +
Sbjct: 202 EGIIVYLSAEEAERLLTDV 208

BLAST of Spg033644 vs. ExPASy Swiss-Prot
Match: B1MGB7 (Putative S-adenosyl-L-methionine-dependent methyltransferase MAB_3787 OS=Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543) OX=561007 GN=MAB_3787 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.2e-15
Identity = 66/191 (34.55%), Postives = 95/191 (49.74%), Query Frame = 0

Query: 45  SACQMAAGRALWKHV----IHDPLAELLAGEACLRNLHEKIKKDRSN--NAREISGVI-- 104
           +A  +AA RAL  HV    I+DP AE L     +    E   K  S   +  +++G++  
Sbjct: 18  TATMVAAQRALASHVPNPLINDPYAEPLVRAVGI----EYFTKLASGAFDPEQMAGLVQL 77

Query: 105 ----------LAVRTLWFDSKIEGALASFNGRDSQVVLLGAGMDTRAYRLSCLKQSDVFE 164
                     +A RT ++D+  E + A+      Q V+L +G+DTRAYRL+    + V+E
Sbjct: 78  GITADGMANGMASRTWYYDTAFESSTAA---GVRQAVILASGLDTRAYRLTWPAGTTVYE 137

Query: 165 VDFPELLQVKATLLKTAIESPYGRHHQQMAKSLNRVAADIRSNDWLEKLQSAGFAPEKST 218
           +D PE++  K   L     SP        +     VA D+R  DW   LQ+AGF PE+ T
Sbjct: 138 LDQPEVITFKTDTLAELGASP--------SAERRTVAIDLR-EDWPAALQAAGFDPEQPT 192

BLAST of Spg033644 vs. ExPASy Swiss-Prot
Match: B1MEP9 (Putative S-adenosyl-L-methionine-dependent methyltransferase MAB_0213c OS=Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC 1543) OX=561007 GN=MAB_0213c PE=3 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.4e-14
Identity = 62/194 (31.96%), Postives = 97/194 (50.00%), Query Frame = 0

Query: 45  SACQMAAGRALWKHVIH----DPLAELL--AGEACLRNLHEKIKKDRSNNAREIS---GV 104
           +A  +AAGRAL +H  +    DP AEL   A      +L +  + D + ++ +       
Sbjct: 17  TALAVAAGRALARHPANGAPIDPYAELFCRAAGGQWSDLVQGKQSDHALSSEDFGRSFAN 76

Query: 105 ILAVRTLWFDSKIEGALASFNGRDSQVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQV 164
             A RT +FD+       + +    QVVLL +G+D RAYRL    +++V+E+D P + Q 
Sbjct: 77  FQAARTAFFDNFF---TTTSDTGVRQVVLLASGLDCRAYRLQWPAETEVYELDQPLVQQF 136

Query: 165 KATLLKTAIESPYGRHHQQMAKSLNRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLY 224
           K   L     +P    H         ++ D+R  DW   LQ +GF P + + W++EGLL+
Sbjct: 137 KQETLDAHGAAPSAVRHP--------ISVDLR-QDWSTILQESGFDPSRPSAWLVEGLLF 196

Query: 225 YLTHSQAMQVLETI 230
           +L  S    +LETI
Sbjct: 197 FLKSSAQDLLLETI 198

BLAST of Spg033644 vs. ExPASy Swiss-Prot
Match: A0QKY8 (Putative S-adenosyl-L-methionine-dependent methyltransferase MAV_4441 OS=Mycobacterium avium (strain 104) OX=243243 GN=MAV_4441 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 4.0e-14
Identity = 62/180 (34.44%), Postives = 90/180 (50.00%), Query Frame = 0

Query: 55  LWKHVIHDPLAELLAGE---ACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGA 114
           L K V  D L+ L  GE   A L ++H+         +R      +AVRT +FD   E  
Sbjct: 47  LVKAVGVDLLSRLAGGELDPAELNDVHDGAAGSAGAMSRMADN--MAVRTKFFD---EFF 106

Query: 115 LASFNGRDSQVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPYGRH 174
           L +     +QVV+L +G+D RAYRL+    + V+EVD P+++  K T L     +P    
Sbjct: 107 LNATKAGIAQVVILASGLDARAYRLAWPAGTVVYEVDQPQVIDFKTTALAQLGAAPTAER 166

Query: 175 HQQMAKSLNRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIAD 232
                     VA D+R +DW   L++AGF P + T W  EGLL YL      ++L+TI +
Sbjct: 167 RV--------VAVDLR-DDWPAALRAAGFDPTRPTAWSAEGLLGYLPPEAQDRLLDTITE 212

BLAST of Spg033644 vs. ExPASy TrEMBL
Match: A0A6J1CRT3 (uncharacterized protein LOC111013987 OS=Momordica charantia OX=3673 GN=LOC111013987 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.5e-181
Identity = 311/337 (92.28%), Postives = 323/337 (95.85%), Query Frame = 0

Query: 1   MTDKENG--LPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKH 60
           MTDKENG  LP+ PGWSELKLPDMLLA+SVRE HARIEAEWDYLQRSACQMAAGRALW H
Sbjct: 1   MTDKENGLRLPEDPGWSELKLPDMLLANSVREAHARIEAEWDYLQRSACQMAAGRALWSH 60

Query: 61  VIHDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGR 120
           VIHDPLA LLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGAL+SFNGR
Sbjct: 61  VIHDPLAGLLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALSSFNGR 120

Query: 121 DSQVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPYGRHHQQMAKS 180
           DSQVVLLGAGMDTRAYRLSCLK+SDVFEVDFPE+LQVK  LLKTAIESPYG HHQQMAKS
Sbjct: 121 DSQVVLLGAGMDTRAYRLSCLKESDVFEVDFPEVLQVKTALLKTAIESPYGHHHQQMAKS 180

Query: 181 LNRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHT 240
           LNR+AADIR NDWLEKLQSAGFA EK+TVWVLEGLLYYLTHSQAMQVLETIADKCTIT T
Sbjct: 181 LNRIAADIRGNDWLEKLQSAGFASEKNTVWVLEGLLYYLTHSQAMQVLETIADKCTITPT 240

Query: 241 VLLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLF 300
           VLLADFMNKPSTTLSNS+F FYCDWPDHLLPSLGFSNTKLSQIGDPDA+FGL+HDPLNLF
Sbjct: 241 VLLADFMNKPSTTLSNSIFHFYCDWPDHLLPSLGFSNTKLSQIGDPDANFGLVHDPLNLF 300

Query: 301 NKIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPPV 336
           NKIRSLPRS+QTHPDDGKPCCRLYLVQASGSP LPP+
Sbjct: 301 NKIRSLPRSMQTHPDDGKPCCRLYLVQASGSPHLPPL 337

BLAST of Spg033644 vs. ExPASy TrEMBL
Match: A0A6J1GI61 (uncharacterized protein LOC111454414 OS=Cucurbita moschata OX=3662 GN=LOC111454414 PE=3 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.5e-181
Identity = 311/336 (92.56%), Postives = 322/336 (95.83%), Query Frame = 0

Query: 1   MTDKENGLPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60
           MT+KENGLPD PGW ELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLPDDPGWFELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDS 120
           HDPLA+LLAGEACLRNLHEKIKKDRSNNARE+SGVILAVRTLWFDSKIEGAL SFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPY-GRHHQQMAKSL 180
           QVVLLGAGMDTRAYRLSCLK+SDVFEVDFPELLQ KA L+K AIESPY   HHQQ+AKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180

Query: 181 NRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTV 240
           NRVAADIRS+DWLEKLQSAGFAPEK+TVWVLEGLLYY THSQAMQVLETIADKC ITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVF FYCDWP+HLLPSLGFS+TKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPEHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPPV 336
           KIRSLPRSLQTHPDDGKPCCRLYLVQASG P LPP+
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGLPLLPPI 336

BLAST of Spg033644 vs. ExPASy TrEMBL
Match: A0A6J1KNW1 (uncharacterized protein LOC111496264 OS=Cucurbita maxima OX=3661 GN=LOC111496264 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 1.8e-179
Identity = 308/336 (91.67%), Postives = 319/336 (94.94%), Query Frame = 0

Query: 1   MTDKENGLPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60
           MT+KENGL D PGW ELK PD+L ADSVREVHARI AEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTEKENGLADDPGWFELKFPDLLSADSVREVHARIVAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDS 120
           HDPLA+LLAGEACLRNLHEKIKKDRSNNARE+SGVILAVRTLWFDSKIEGAL SFNGRDS
Sbjct: 61  HDPLADLLAGEACLRNLHEKIKKDRSNNAREMSGVILAVRTLWFDSKIEGALTSFNGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPY-GRHHQQMAKSL 180
           QVVLLGAGMDTRAYRLSCLK+SDVFEVDFPELLQ KA L+K AIESPY   HHQQ+AKSL
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFPELLQAKANLIKIAIESPYEHHHHQQIAKSL 180

Query: 181 NRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTV 240
           NRVAADIRS+DWLEKLQSAGFAPEK+TVWVLEGLLYY THSQAMQVLETIADKC ITHTV
Sbjct: 181 NRVAADIRSSDWLEKLQSAGFAPEKNTVWVLEGLLYYFTHSQAMQVLETIADKCNITHTV 240

Query: 241 LLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFN 300
           LLADFMNKPSTTLSNSVF FYCDWPDHLLPSLGFS+TKLSQIGDPDAHFGLMHDPLNLFN
Sbjct: 241 LLADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSSTKLSQIGDPDAHFGLMHDPLNLFN 300

Query: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPPV 336
           KIRSLPRSLQTHPDDGKPCCRLYLVQASGSP LPP+
Sbjct: 301 KIRSLPRSLQTHPDDGKPCCRLYLVQASGSPLLPPI 336

BLAST of Spg033644 vs. ExPASy TrEMBL
Match: A0A6J1KE32 (uncharacterized protein LOC111492397 OS=Cucurbita maxima OX=3661 GN=LOC111492397 PE=3 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 5.4e-179
Identity = 307/334 (91.92%), Postives = 315/334 (94.31%), Query Frame = 0

Query: 1   MTDKENGLPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60
           MTDKENG PD PGWSELKLPD+LL DSVRE+HARIEAEWDYLQRSACQMAAGRALWKHVI
Sbjct: 1   MTDKENGWPDNPGWSELKLPDLLLVDSVREIHARIEAEWDYLQRSACQMAAGRALWKHVI 60

Query: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDS 120
           HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGAL S NGRDS
Sbjct: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALTSINGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPYGRHHQQMAKSLN 180
           QVVLLGAGMDTRAYRLSCLK+SDVFEVDF ELLQ KATLL  AIE P G HHQQ AKSLN
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFSELLQAKATLLNAAIEPPNGHHHQQKAKSLN 180

Query: 181 RVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTVL 240
           RVAADIRSNDWLEKL++AGFA EK+TVWVLEGLLYYLTHSQA+QVLETIAD+CTITHTVL
Sbjct: 181 RVAADIRSNDWLEKLKTAGFASEKNTVWVLEGLLYYLTHSQALQVLETIADQCTITHTVL 240

Query: 241 LADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFNK 300
           LADFMNKPSTTLSNSVF FYCDWPDHLLPSLGFS T LSQIGD DAHFGLMHDPLNLFNK
Sbjct: 241 LADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSTTTLSQIGDMDAHFGLMHDPLNLFNK 300

Query: 301 IRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPP 335
           IRSLPRSLQTHPDDGKPCCRLYLVQASGSPQL P
Sbjct: 301 IRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLQP 334

BLAST of Spg033644 vs. ExPASy TrEMBL
Match: A0A6J1HBI5 (uncharacterized protein LOC111462501 OS=Cucurbita moschata OX=3662 GN=LOC111462501 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.2e-178
Identity = 306/334 (91.62%), Postives = 317/334 (94.91%), Query Frame = 0

Query: 1   MTDKENGLPDGPGWSELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVI 60
           MTDKENG PD PGWSELKLPD+LL DSVRE+HARIEAEWDYLQRSACQMAAGRALWKH+I
Sbjct: 1   MTDKENGWPDNPGWSELKLPDLLLVDSVREIHARIEAEWDYLQRSACQMAAGRALWKHMI 60

Query: 61  HDPLAELLAGEACLRNLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDS 120
           HDPLAELLAGEACLRNL+EKIKKDRSNNAREISGVILAVRTLWFDSKIEGAL S +GRDS
Sbjct: 61  HDPLAELLAGEACLRNLYEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALTSISGRDS 120

Query: 121 QVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLLKTAIESPYGRHHQQMAKSLN 180
           QVVLLGAGMDTRAYRLSCLK+SDVFEVDF ELLQ KATLLK AIE P G HHQQ AKSLN
Sbjct: 121 QVVLLGAGMDTRAYRLSCLKESDVFEVDFSELLQAKATLLKAAIEPPNGHHHQQKAKSLN 180

Query: 181 RVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTVL 240
           RVAADIRSNDWLEKL++AGFA EK+TVWVLEGLLYYLTHSQA+QVLETIAD+CTITHTVL
Sbjct: 181 RVAADIRSNDWLEKLKTAGFASEKNTVWVLEGLLYYLTHSQALQVLETIADQCTITHTVL 240

Query: 241 LADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFNK 300
           LADFMNKPSTTLSNSVF FYCDWPDHLLPSLGFSNT LSQIGD DAHFGLMHDPLNLFNK
Sbjct: 241 LADFMNKPSTTLSNSVFHFYCDWPDHLLPSLGFSNTTLSQIGDMDAHFGLMHDPLNLFNK 300

Query: 301 IRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLPP 335
           IRSLPRSLQTHPDDGKPCCRLYLVQASGSPQL P
Sbjct: 301 IRSLPRSLQTHPDDGKPCCRLYLVQASGSPQLQP 334

BLAST of Spg033644 vs. TAIR 10
Match: AT5G42760.1 (Leucine carboxyl methyltransferase )

HSP 1 Score: 460.3 bits (1183), Expect = 1.3e-129
Identity = 220/316 (69.62%), Postives = 264/316 (83.54%), Query Frame = 0

Query: 16  ELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVIHDPLAELLAGEACLR 75
           EL LPD+L ++ V+++H  I++EWDYLQ+SACQ AAGRALWKHVIHDPLA L AGE  LR
Sbjct: 28  ELVLPDVLRSEGVKKLHMSIQSEWDYLQKSACQTAAGRALWKHVIHDPLAHLFAGETHLR 87

Query: 76  NLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDSQVVLLGAGMDTRAYR 135
           NLH KI+ DR NNARE+SGVILAVRTLWFD++I+ AL SF+G  +QVVLLGAGMD R+YR
Sbjct: 88  NLHTKIQTDRLNNAREVSGVILAVRTLWFDTRIQAALESFHGDAAQVVLLGAGMDARSYR 147

Query: 136 LSCLKQSDVFEVDFPELLQVKATLLKTAIESPYGRHHQQM-AKSLNRVAADIRSNDWLEK 195
           L+CL +SDVFEVDF ++L+ KA+L++ A+ S   R   +M AKSL RVA DIR NDW E+
Sbjct: 148 LNCLNKSDVFEVDFQDVLETKASLVQAAVNS---RDELRMTAKSLVRVAIDIRDNDWFEQ 207

Query: 196 LQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTVLLADFMNKPSTTLSN 255
           L+ +GF PE +TVWVLEG+LYYL+H++AMQVL  IA+KC +T TVLLADFMNKPS TL N
Sbjct: 208 LKKSGFLPEINTVWVLEGILYYLSHTEAMQVLNLIAEKCGLTSTVLLADFMNKPSATLPN 267

Query: 256 SVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFNKIRSLPRSLQTHPDD 315
           SVF FY DWPD LLP+LGFS+ KLSQIGDPDA+FGL+HDP NLFNK+  LPR+ Q HPDD
Sbjct: 268 SVFHFYSDWPDQLLPTLGFSHVKLSQIGDPDANFGLLHDPRNLFNKLLRLPRTAQIHPDD 327

Query: 316 GKPCCRLYLVQASGSP 331
           GKPCCRLYLV+ASGSP
Sbjct: 328 GKPCCRLYLVEASGSP 340

BLAST of Spg033644 vs. TAIR 10
Match: AT5G42760.2 (Leucine carboxyl methyltransferase )

HSP 1 Score: 448.7 bits (1153), Expect = 4.0e-126
Identity = 215/313 (68.69%), Postives = 258/313 (82.43%), Query Frame = 0

Query: 16  ELKLPDMLLADSVREVHARIEAEWDYLQRSACQMAAGRALWKHVIHDPLAELLAGEACLR 75
           EL LPD+L ++ V+++H  I++EWDYLQ+SACQ AAGRALWKHVIHDPLA L AGE  LR
Sbjct: 24  ELVLPDVLRSEGVKKLHMSIQSEWDYLQKSACQTAAGRALWKHVIHDPLAHLFAGETHLR 83

Query: 76  NLHEKIKKDRSNNAREISGVILAVRTLWFDSKIEGALASFNGRDSQVVLLGAGMDTRAYR 135
           NLH KI+ DR NNARE+SGVILAVRTLWFD++I+ AL SF+G  +QVVLLGAGMD R+YR
Sbjct: 84  NLHTKIQTDRLNNAREVSGVILAVRTLWFDTRIQAALESFHGDAAQVVLLGAGMDARSYR 143

Query: 136 LSCLKQSDVFEVDFPELLQVKATLLKTAIESPYGRHHQQM-AKSLNRVAADIRSNDWLEK 195
           L+CL +SDVFEVDF ++L+ KA+L++ A+ S   R   +M AKSL RVA DIR NDW E+
Sbjct: 144 LNCLNKSDVFEVDFQDVLETKASLVQAAVNS---RDELRMTAKSLVRVAIDIRDNDWFEQ 203

Query: 196 LQSAGFAPEKSTVWVLEGLLYYLTHSQAMQVLETIADKCTITHTVLLADFMNKPSTTLSN 255
           L+ +GF PE +TVWVLEG+LYYL+H++AMQVL  IA+KC +T TVLLADFMNKPS TL N
Sbjct: 204 LKKSGFLPEINTVWVLEGILYYLSHTEAMQVLNLIAEKCGLTSTVLLADFMNKPSATLPN 263

Query: 256 SVFQFYCDWPDHLLPSLGFSNTKLSQIGDPDAHFGLMHDPLNLFNKIRSLPRSLQTHPDD 315
           SVF FY DWPD LLP+LGFS+ KLSQIGDPDA+FGL+HDP NLFNK+  LPR+ Q HPDD
Sbjct: 264 SVFHFYSDWPDQLLPTLGFSHVKLSQIGDPDANFGLLHDPRNLFNKLLRLPRTAQIHPDD 323

Query: 316 GKPCCRLYLVQAS 328
           GKPCCRLYL   S
Sbjct: 324 GKPCCRLYLFYIS 333

BLAST of Spg033644 vs. TAIR 10
Match: AT4G02405.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 1.3e-04
Identity = 43/197 (21.83%), Postives = 85/197 (43.15%), Query Frame = 0

Query: 101 TLWFDSKIEGALASFNGRDSQVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLL 160
           T + D K+       +G   QVVL   GMDTR YRL+    + +F+V   ++ ++ +  L
Sbjct: 115 TKFIDDKLLEIAKRIDGL-KQVVLFTDGMDTRPYRLNWPTSTMIFDVSPEKVFEIASEKL 174

Query: 161 KTAIESPYGRHHQQMAKSLNRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHS 220
           +            ++ KS       +   +  ++L+S GF+  + ++W ++GL   L   
Sbjct: 175 QGV--------GARIPKSCLFFHIPVEFENIEQRLRSKGFSGNRPSIWAMQGL--PLESQ 234

Query: 221 QAMQVLETIADKCTITHTVLLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQ 280
              + + +      +    L+ +     + TL + + +    W + L  S GF   K+  
Sbjct: 235 SGFEAILSAISSLAMNECYLIGEL--PTNITLQSDLSK----WMEKLFMSNGF-RVKIVS 293

Query: 281 IGDPDAHFG-LMHDPLN 297
             +  A  G ++H P+N
Sbjct: 295 YEEIAASLGVVLHSPVN 293

BLAST of Spg033644 vs. TAIR 10
Match: AT4G02405.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 45.1 bits (105), Expect = 1.3e-04
Identity = 43/197 (21.83%), Postives = 85/197 (43.15%), Query Frame = 0

Query: 101 TLWFDSKIEGALASFNGRDSQVVLLGAGMDTRAYRLSCLKQSDVFEVDFPELLQVKATLL 160
           T + D K+       +G   QVVL   GMDTR YRL+    + +F+V   ++ ++ +  L
Sbjct: 115 TKFIDDKLLEIAKRIDGL-KQVVLFTDGMDTRPYRLNWPTSTMIFDVSPEKVFEIASEKL 174

Query: 161 KTAIESPYGRHHQQMAKSLNRVAADIRSNDWLEKLQSAGFAPEKSTVWVLEGLLYYLTHS 220
           +            ++ KS       +   +  ++L+S GF+  + ++W ++GL   L   
Sbjct: 175 QGV--------GARIPKSCLFFHIPVEFENIEQRLRSKGFSGNRPSIWAMQGL--PLESQ 234

Query: 221 QAMQVLETIADKCTITHTVLLADFMNKPSTTLSNSVFQFYCDWPDHLLPSLGFSNTKLSQ 280
              + + +      +    L+ +     + TL + + +    W + L  S GF   K+  
Sbjct: 235 SGFEAILSAISSLAMNECYLIGEL--PTNITLQSDLSK----WMEKLFMSNGF-RVKIVS 293

Query: 281 IGDPDAHFG-LMHDPLN 297
             +  A  G ++H P+N
Sbjct: 295 YEEIAASLGVVLHSPVN 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023537171.12.8e-18293.15uncharacterized protein LOC111798329 [Cucurbita pepo subsp. pepo][more]
KAG7020511.12.4e-18192.56hypothetical protein SDJN02_17196 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022951671.13.1e-18192.56uncharacterized protein LOC111454414 [Cucurbita moschata][more]
XP_022144259.13.1e-18192.28uncharacterized protein LOC111013987 [Momordica charantia] >XP_022144260.1 uncha... [more]
KAG7029387.12.9e-17991.92hypothetical protein SDJN02_07726, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
B2HD962.1e-1533.33Putative S-adenosyl-L-methionine-dependent methyltransferase MMAR_2791 OS=Mycoba... [more]
A8LHA42.8e-1535.25Putative S-adenosyl-L-methionine-dependent methyltransferase Franean1_4929 OS=Fr... [more]
B1MGB76.2e-1534.55Putative S-adenosyl-L-methionine-dependent methyltransferase MAB_3787 OS=Mycobac... [more]
B1MEP92.4e-1431.96Putative S-adenosyl-L-methionine-dependent methyltransferase MAB_0213c OS=Mycoba... [more]
A0QKY84.0e-1434.44Putative S-adenosyl-L-methionine-dependent methyltransferase MAV_4441 OS=Mycobac... [more]
Match NameE-valueIdentityDescription
A0A6J1CRT31.5e-18192.28uncharacterized protein LOC111013987 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J1GI611.5e-18192.56uncharacterized protein LOC111454414 OS=Cucurbita moschata OX=3662 GN=LOC1114544... [more]
A0A6J1KNW11.8e-17991.67uncharacterized protein LOC111496264 OS=Cucurbita maxima OX=3661 GN=LOC111496264... [more]
A0A6J1KE325.4e-17991.92uncharacterized protein LOC111492397 OS=Cucurbita maxima OX=3661 GN=LOC111492397... [more]
A0A6J1HBI51.2e-17891.62uncharacterized protein LOC111462501 OS=Cucurbita moschata OX=3662 GN=LOC1114625... [more]
Match NameE-valueIdentityDescription
AT5G42760.11.3e-12969.62Leucine carboxyl methyltransferase [more]
AT5G42760.24.0e-12668.69Leucine carboxyl methyltransferase [more]
AT4G02405.11.3e-0421.83S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G02405.21.3e-0421.83S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011610S-adenosyl-L-methionine-dependent methyltransferase, putativeTIGRFAMTIGR00027TIGR00027coord: 50..234
e-value: 3.2E-39
score: 132.9
IPR007213Methyltransferase Ppm1/Ppm2/TcmpPFAMPF04072LCMcoord: 46..230
e-value: 2.9E-49
score: 167.6
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 37..287
e-value: 1.4E-49
score: 171.0
NoneNo IPR availablePIRSRPIRSR016305-1PIRSR016305-1coord: 99..240
e-value: 5.2E-13
score: 46.5
NoneNo IPR availablePANTHERPTHR43619:SF8LEUCINE CARBOXYL METHYLTRANSFERASEcoord: 16..325
NoneNo IPR availablePANTHERPTHR43619S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE YKTD-RELATEDcoord: 16..325
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 43..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg033644.1Spg033644.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity