Spg033600 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg033600 vs. NCBI nr
Match: XP_023527355.1 (influenza virus NS1A-binding protein homolog [Cucurbita pepo subsp. pepo] >XP_023527356.1 influenza virus NS1A-binding protein homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 969.1 bits (2504), Expect = 1.8e-278
Identity = 504/677 (74.45%), Postives = 552/677 (81.54%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNTG++ALKFKAGSS+RL+ G V RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLP+QHF
Sbjct: 1   MNTGQRALKFKAGSSSRLDAGEVYRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPSQHF 60

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYVKNID GLPLFLFNYSERKL+GIFEAASSGQ+NINPYGWTTDGSERTLYPAQVQILVR
Sbjct: 61  SYVKNIDPGLPLFLFNYSERKLNGIFEAASSGQININPYGWTTDGSERTLYPAQVQILVR 120

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
           K CQPLLENQFKP+I DNYY P+HFWFELDHAQTNKL+SLL+SQA+ PHVPQ TT  +  
Sbjct: 121 KLCQPLLENQFKPIIKDNYYSPNHFWFELDHAQTNKLISLLSSQAMTPHVPQFTTNCKPC 180

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C  LP LE+ DE EKIK QI+E+HF L SQV D VDV SSLDAGNSA G H  ANE++EE
Sbjct: 181 CTVLPSLESRDEGEKIKSQIMEEHFDLESQVVDAVDVTSSLDAGNSALGAHCYANESDEE 240

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
            K R+LHKLQQ AI HHES +LP TSD NHTT N+  NL NN  SGEPIKS+ESNEED G
Sbjct: 241 VKCRILHKLQQFAIKHHESPMLPLTSDMNHTTINKGNNLVNNGSSGEPIKSKESNEEDFG 300

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMMS-YLPNSN 360
           +LT  QS IAKLVQE QELK+S AEQA++IV LEEKLLAAE+EI ELK R+ S YLPNSN
Sbjct: 301 TLTGLQSPIAKLVQELQELKESNAEQARRIVFLEEKLLAAEDEIQELKGRITSNYLPNSN 360

Query: 361 AVEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRA 420
           AVEA+R +VEEQ  D CLDP+ESIFLIGGYDG SHLSTL LYDPS D+IKSL PM SVRA
Sbjct: 361 AVEAKRVVVEEQLEDLCLDPDESIFLIGGYDGASHLSTLELYDPSGDMIKSLRPMSSVRA 420

Query: 421 YASVAWLNGQLYVFGGGNGYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFA 480
           YASVAWLN QLYVFGGGNG AWY+TVESY+LETD+W+ CPSLN+AKGSLGGV+IGNKLFA
Sbjct: 421 YASVAWLNSQLYVFGGGNGCAWYNTVESYNLETDQWTLCPSLNLAKGSLGGVSIGNKLFA 480

Query: 481 IGGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLY 540
           IGGGNGIESFSDVEMLD+DLGRWICTRSMLQR              RFA+AAVELNGV+Y
Sbjct: 481 IGGGNGIESFSDVEMLDMDLGRWICTRSMLQR--------------RFAVAAVELNGVIY 540

Query: 541 ATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVST 600
           ATGGFDG+DY++SAERFDIREHSWTQIP+MNAKRGCHSLV+LNEKLYALGGFDGDSMVS+
Sbjct: 541 ATGGFDGNDYMRSAERFDIREHSWTQIPNMNAKRGCHSLVTLNEKLYALGGFDGDSMVSS 600

Query: 601 VE----------------------------------------------VESYKEGCGWQE 629
           VE                                              VESYKEGCGWQE
Sbjct: 601 VEVYDPRMESWIVGEPMNKTRGYAAAGVINESIYIIGGVLIDDKILNTVESYKEGCGWQE 660

BLAST of Spg033600 vs. NCBI nr
Match: XP_022982773.1 (influenza virus NS1A-binding protein homolog [Cucurbita maxima] >XP_022982774.1 influenza virus NS1A-binding protein homolog [Cucurbita maxima])

HSP 1 Score: 968.4 bits (2502), Expect = 3.0e-278
Identity = 506/676 (74.85%), Postives = 550/676 (81.36%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNTG++ALKFKAGSSARL+   V RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLP+QHF
Sbjct: 1   MNTGQRALKFKAGSSARLDADEVYRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPSQHF 60

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYVKNID GLPLFLFNYSERKL+GIFEAASSGQ+NINPYGWTTDGSERTLYPAQVQILVR
Sbjct: 61  SYVKNIDPGLPLFLFNYSERKLNGIFEAASSGQININPYGWTTDGSERTLYPAQVQILVR 120

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
           K CQPLLENQFKP+I DNYY P+HFWFELDHAQTNKL+SLL+SQA+ PHVPQ TT  R  
Sbjct: 121 KLCQPLLENQFKPIIKDNYYSPNHFWFELDHAQTNKLISLLSSQAMTPHVPQFTTNCRPC 180

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C  LP LE+ DE EKIK QI+E+HF L SQV D VDV SSLDAGNS+ G H  ANE++EE
Sbjct: 181 CTVLPSLESRDEGEKIKSQIMEEHFDLESQVVDAVDVTSSLDAGNSSLGAHCYANESDEE 240

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
            K R+LHKLQQ AI HHESS+LP TSD NHTT N+  NL NN  SGEPIKS+ESNEED G
Sbjct: 241 LKCRILHKLQQFAIKHHESSMLPLTSDMNHTTINKGNNLVNNGSSGEPIKSKESNEEDFG 300

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMMS-YLPNSN 360
           +LT  QS IAKLVQE QELKDS AEQA++IV LEEKLLAAE EI ELK R+ S YLPNSN
Sbjct: 301 TLTGLQSPIAKLVQELQELKDSNAEQARRIVFLEEKLLAAEGEIQELKGRITSNYLPNSN 360

Query: 361 AVEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRA 420
           AVEA+R +VEEQ  D CLDP+ESIFLIGGYDG SHLSTL LYDPS D+IKSL PM SVRA
Sbjct: 361 AVEAKRVVVEEQLEDLCLDPDESIFLIGGYDGASHLSTLELYDPSSDMIKSLRPMSSVRA 420

Query: 421 YASVAWLNGQLYVFGGGNGYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFA 480
           YASVAWLN QLYVFGGGNG AWY+TVESY+LETD+W+ CPSLN+AKGSLGGV+IGNKLFA
Sbjct: 421 YASVAWLNSQLYVFGGGNGCAWYNTVESYNLETDQWTLCPSLNLAKGSLGGVSIGNKLFA 480

Query: 481 IGGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLY 540
           IGGGNGIESFS VEMLD++LGRWICTRSMLQR              RFA+AAVELNGVLY
Sbjct: 481 IGGGNGIESFSGVEMLDMELGRWICTRSMLQR--------------RFAVAAVELNGVLY 540

Query: 541 ATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVST 600
           ATGGFDG+DY++SAERFDIREHSWTQIPSMNAKRGCHSLV+LNEKLYALGGFDGDSMVS+
Sbjct: 541 ATGGFDGNDYMRSAERFDIREHSWTQIPSMNAKRGCHSLVTLNEKLYALGGFDGDSMVSS 600

Query: 601 VE----------------------------------------------VESYKEGCGWQE 628
           VE                                              VESYKEGCGWQE
Sbjct: 601 VEVYDPRMESWIDGEPMNKTRGYAAAGVINESIYIIGGVLVDDKILNTVESYKEGCGWQE 660

BLAST of Spg033600 vs. NCBI nr
Match: KAG6580447.1 (Kelch-like protein 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 961.1 bits (2483), Expect = 4.8e-276
Identity = 502/677 (74.15%), Postives = 550/677 (81.24%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNTG++ALKFKAGSS+RL+ G V RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLP+QHF
Sbjct: 32  MNTGQRALKFKAGSSSRLDAGEVYRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPSQHF 91

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYVKNID GLPLFLFNYSERKL+GIFEAASSGQ+NINPYGWTTDGSERTLYPAQVQILVR
Sbjct: 92  SYVKNIDPGLPLFLFNYSERKLNGIFEAASSGQININPYGWTTDGSERTLYPAQVQILVR 151

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
           K CQPLLENQFKP+I DNYY P+HFWFELDHAQTNKL+SLL+SQA+  HVPQ TT  +  
Sbjct: 152 KLCQPLLENQFKPIIKDNYYSPNHFWFELDHAQTNKLISLLSSQAMTLHVPQFTTNCKPC 211

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C  LP LE+ DE EKIK QI+E+HF L SQV D VDV SSLDAGNSA G H  ANE++EE
Sbjct: 212 CTVLPSLESRDEGEKIKSQIMEEHFDLESQVVDAVDVTSSLDAGNSALGAHCYANESDEE 271

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
            K R+LHKLQQ AI HHES +LP TSD NHTT N+  NL NN  SGEPIKS+ES+EED G
Sbjct: 272 VKCRILHKLQQFAIKHHESPMLPLTSDMNHTTINKGNNLVNNGSSGEPIKSKESDEEDFG 331

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMMS-YLPNSN 360
           +LT  QS IAKLVQE QELK+S AEQA++IV LEEKLLAAE EI ELK R+ S  LPNSN
Sbjct: 332 TLTGLQSPIAKLVQELQELKESNAEQARRIVFLEEKLLAAEGEIQELKGRITSNNLPNSN 391

Query: 361 AVEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRA 420
           AVEA+R +VEEQ  D CLDP+ESIFLIGGYDG SHLSTL LYDPS D+IKSL PM SVRA
Sbjct: 392 AVEAKRVVVEEQLEDLCLDPDESIFLIGGYDGASHLSTLELYDPSSDMIKSLRPMSSVRA 451

Query: 421 YASVAWLNGQLYVFGGGNGYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFA 480
           YASVAWLN QLYVFGGGNG AWY+TVESY+LETD+W+ CPSLN+AKGSLGGV+IGNKLFA
Sbjct: 452 YASVAWLNSQLYVFGGGNGCAWYNTVESYNLETDQWTLCPSLNLAKGSLGGVSIGNKLFA 511

Query: 481 IGGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLY 540
           IGGGNGIESFSDVEMLD+DLGRWICTRSMLQR              RFA+AAVELNGVLY
Sbjct: 512 IGGGNGIESFSDVEMLDMDLGRWICTRSMLQR--------------RFAVAAVELNGVLY 571

Query: 541 ATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVST 600
           ATGGFDG+DY++SAERFDIREHSWTQIP+MNAKRGCHSLV+LNEKLYA+GGFDGDSMVS+
Sbjct: 572 ATGGFDGNDYMRSAERFDIREHSWTQIPNMNAKRGCHSLVTLNEKLYAVGGFDGDSMVSS 631

Query: 601 VE----------------------------------------------VESYKEGCGWQE 629
           VE                                              VESYKEGCGWQE
Sbjct: 632 VEVYDPRMESWIVGEPMNKTRGYAAAGVINESIYIIGGVLIDDKILNTVESYKEGCGWQE 691

BLAST of Spg033600 vs. NCBI nr
Match: XP_022934311.1 (influenza virus NS1A-binding protein homolog [Cucurbita moschata] >XP_022934312.1 influenza virus NS1A-binding protein homolog [Cucurbita moschata] >XP_022934314.1 influenza virus NS1A-binding protein homolog [Cucurbita moschata])

HSP 1 Score: 961.1 bits (2483), Expect = 4.8e-276
Identity = 502/677 (74.15%), Postives = 550/677 (81.24%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNTG++ALKFKAGSS+RL+ G V RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLP+QHF
Sbjct: 1   MNTGQRALKFKAGSSSRLDAGEVYRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPSQHF 60

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYVKNID GLPLFLFNYSERKL+GIFEAASSGQ+NINPYGWTTDGSERTLYPAQVQILVR
Sbjct: 61  SYVKNIDPGLPLFLFNYSERKLNGIFEAASSGQININPYGWTTDGSERTLYPAQVQILVR 120

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
           K CQPLLENQFKP+I DNYY P+HFWFELDHAQTNKL+SLL+SQA+  HVPQ TT  +  
Sbjct: 121 KLCQPLLENQFKPIIKDNYYSPNHFWFELDHAQTNKLISLLSSQAMTLHVPQFTTNCKPC 180

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C  LP LE+ DE EKIK QI+E+HF L SQV D VDV SSLDAGNSA G H  ANE++EE
Sbjct: 181 CTVLPSLESRDEGEKIKSQIMEEHFDLESQVVDAVDVTSSLDAGNSALGAHCYANESDEE 240

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
            K R+LHKLQQ AI HHES +LP TSD NHTT N+  NL NN  SGEPIKS+ES+EED G
Sbjct: 241 VKCRILHKLQQFAIKHHESPMLPLTSDMNHTTINKGNNLVNNGSSGEPIKSKESDEEDFG 300

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMMS-YLPNSN 360
           +LT  QS IAKLVQE QELK+S AEQA++IV LEEKLLAAE EI ELK R+ S  LPNSN
Sbjct: 301 TLTGLQSPIAKLVQELQELKESNAEQARRIVFLEEKLLAAEGEIQELKGRITSNNLPNSN 360

Query: 361 AVEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRA 420
           AVEA+R +VEEQ  D CLDP+ESIFLIGGYDG SHLSTL LYDPS D+IKSL PM SVRA
Sbjct: 361 AVEAKRVVVEEQLEDLCLDPDESIFLIGGYDGASHLSTLELYDPSSDMIKSLRPMSSVRA 420

Query: 421 YASVAWLNGQLYVFGGGNGYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFA 480
           YASVAWLN QLYVFGGGNG AWY+TVESY+LETD+W+ CPSLN+AKGSLGGV+IGNKLFA
Sbjct: 421 YASVAWLNSQLYVFGGGNGCAWYNTVESYNLETDQWTLCPSLNLAKGSLGGVSIGNKLFA 480

Query: 481 IGGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLY 540
           IGGGNGIESFSDVEMLD+DLGRWICTRSMLQR              RFA+AAVELNGVLY
Sbjct: 481 IGGGNGIESFSDVEMLDMDLGRWICTRSMLQR--------------RFAVAAVELNGVLY 540

Query: 541 ATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVST 600
           ATGGFDG+DY++SAERFDIREHSWTQIP+MNAKRGCHSLV+LNEKLYA+GGFDGDSMVS+
Sbjct: 541 ATGGFDGNDYMRSAERFDIREHSWTQIPNMNAKRGCHSLVTLNEKLYAVGGFDGDSMVSS 600

Query: 601 VE----------------------------------------------VESYKEGCGWQE 629
           VE                                              VESYKEGCGWQE
Sbjct: 601 VEVYDPRMESWIVGEPMNKTRGYAAAGVINESIYIIGGVLIDDKILNTVESYKEGCGWQE 660

BLAST of Spg033600 vs. NCBI nr
Match: KAG7017201.1 (Kelch-like protein 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 957.2 bits (2473), Expect = 6.9e-275
Identity = 501/677 (74.00%), Postives = 549/677 (81.09%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNTG++ALKFKAGSS+RL+ G V RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLP+QHF
Sbjct: 1   MNTGQRALKFKAGSSSRLDAGEVYRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPSQHF 60

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYVKNID GLPLFLFNYSERKL+GIFEAASSGQ+NINPYGWTTDGSERTLYPAQVQILVR
Sbjct: 61  SYVKNIDPGLPLFLFNYSERKLNGIFEAASSGQININPYGWTTDGSERTLYPAQVQILVR 120

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
           K CQPLLENQFKP+I DNYY P+HFWFELDHAQTNKL+SLL+SQA+  HVPQ TT  +  
Sbjct: 121 KLCQPLLENQFKPIIKDNYYSPNHFWFELDHAQTNKLISLLSSQAMTLHVPQFTTNCKPC 180

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C  L  LE+ DE EKIK QI+E+HF L SQV D VDV SSLDAGNSA G H  ANE++EE
Sbjct: 181 CTVLLSLESRDEGEKIKSQIMEEHFDLESQVVDAVDVTSSLDAGNSALGAHCYANESDEE 240

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
            K R+LHKLQQ AI HHES +LP TSD NHTT N+  NL NN  SGEPIKS+ES+EED G
Sbjct: 241 VKCRILHKLQQFAIKHHESPMLPLTSDMNHTTINKGNNLVNNGSSGEPIKSKESDEEDFG 300

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMMS-YLPNSN 360
           +LT  QS IAKLVQE QELK+S AEQA++IV LEEKLLAAE EI ELK R+ S  LPNSN
Sbjct: 301 TLTGLQSPIAKLVQELQELKESNAEQARRIVFLEEKLLAAEGEIQELKGRITSNNLPNSN 360

Query: 361 AVEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRA 420
           AVEA+R +VEEQ  D CLDP+ESIFLIGGYDG SHLSTL LYDPS D+IKSL PM SVRA
Sbjct: 361 AVEAKRVVVEEQLEDLCLDPDESIFLIGGYDGASHLSTLELYDPSSDMIKSLRPMSSVRA 420

Query: 421 YASVAWLNGQLYVFGGGNGYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFA 480
           YASVAWLN QLYVFGGGNG AWY+TVESY+LETD+W+ CPSLN+AKGSLGGV+IGNKLFA
Sbjct: 421 YASVAWLNSQLYVFGGGNGCAWYNTVESYNLETDQWTLCPSLNLAKGSLGGVSIGNKLFA 480

Query: 481 IGGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLY 540
           IGGGNGIESFSDVEMLD+DLGRWICTRSMLQR              RFA+AAVELNGVLY
Sbjct: 481 IGGGNGIESFSDVEMLDMDLGRWICTRSMLQR--------------RFAVAAVELNGVLY 540

Query: 541 ATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVST 600
           ATGGFDG+DY++SAERFDIREHSWTQIP+MNAKRGCHSLV+LNEKLYA+GGFDGDSMVS+
Sbjct: 541 ATGGFDGNDYMRSAERFDIREHSWTQIPNMNAKRGCHSLVTLNEKLYAVGGFDGDSMVSS 600

Query: 601 VE----------------------------------------------VESYKEGCGWQE 629
           VE                                              VESYKEGCGWQE
Sbjct: 601 VEVYDPRMESWIVGEPMNKTRGYAAAGVINESIYIIGGVLIDDKILNTVESYKEGCGWQE 660

BLAST of Spg033600 vs. ExPASy Swiss-Prot
Match: Q9P2G9 (Kelch-like protein 8 OS=Homo sapiens OX=9606 GN=KLHL8 PE=1 SV=4)

HSP 1 Score: 142.1 bits (357), Expect = 2.1e-32
Identity = 72/221 (32.58%), Postives = 122/221 (55.20%), Query Frame = 0

Query: 381 IFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRAYASVAWLNGQLYVFGGGNGYAWY 440
           ++ +GG+DG  HL ++ ++DP  +       M + R   ++A L G +Y  GG +    +
Sbjct: 368 VYAVGGHDGNEHLGSMEMFDPLTNKWMMKASMNTKRRGIALASLGGPIYAIGGLDDNTCF 427

Query: 441 DTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFAIGGGNGIESFSDVEMLDLDLGRW 500
           + VE Y +E+D+WS+   +N  +G +G V + N ++A+GG +G+ S S VE  D  L +W
Sbjct: 428 NDVERYDIESDQWSTVAPMNTPRGGVGSVALVNHVYAVGGNDGMASLSSVERYDPHLDKW 487

Query: 501 ICTRSMLQR--VNGVSSMFVMFNFKRFALAAVELNGVLYATGGFDGSDYLKSAERFDIRE 560
           I  + M QR   NGVS                +L+G LY  GGFD +  L S ER+D R 
Sbjct: 488 IEVKEMGQRRAGNGVS----------------KLHGCLYVVGGFDDNSPLSSVERYDPRS 547

Query: 561 HSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVSTVE 600
           + W  + ++   RG   + ++  K++A+GG +G++ ++TVE
Sbjct: 548 NKWDYVAALTTPRGGVGIATVMGKIFAVGGHNGNAYLNTVE 572

BLAST of Spg033600 vs. ExPASy Swiss-Prot
Match: P59280 (Kelch-like protein 8 OS=Mus musculus OX=10090 GN=Klhl8 PE=2 SV=2)

HSP 1 Score: 141.0 bits (354), Expect = 4.7e-32
Identity = 72/221 (32.58%), Postives = 120/221 (54.30%), Query Frame = 0

Query: 381 IFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRAYASVAWLNGQLYVFGGGNGYAWY 440
           ++ +GG+DG  HL ++ ++DP  +       M + R   ++A L G +Y  GG +    +
Sbjct: 377 VYAVGGHDGNEHLGSMEMFDPLTNKWMMKASMNTKRRGIALASLGGPIYAIGGLDDNTCF 436

Query: 441 DTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFAIGGGNGIESFSDVEMLDLDLGRW 500
             VE Y +E+D+WS+   +N  +G +G V + N ++A+GG +G+ S S VE     L +W
Sbjct: 437 SDVERYDIESDQWSTVAPMNTPRGGVGSVALINHVYAVGGNDGVASLSSVERYHPHLDKW 496

Query: 501 ICTRSMLQR--VNGVSSMFVMFNFKRFALAAVELNGVLYATGGFDGSDYLKSAERFDIRE 560
           I  + M QR   NGVS                EL+G LY  GGFD +  L S ER+D R 
Sbjct: 497 IEVKEMGQRRAGNGVS----------------ELHGCLYVVGGFDDNSPLSSVERYDPRS 556

Query: 561 HSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVSTVE 600
           + W  + ++   RG   + ++  K++A+GG +G++ ++TVE
Sbjct: 557 NKWDYVAALTTPRGGVGIATVMGKIFAVGGHNGNAYLNTVE 581

BLAST of Spg033600 vs. ExPASy Swiss-Prot
Match: G5ED84 (Kelch-like protein 8 OS=Caenorhabditis elegans OX=6239 GN=kel-8 PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 5.2e-31
Identity = 76/222 (34.23%), Postives = 113/222 (50.90%), Query Frame = 0

Query: 378 NESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRAYASVAWLNGQLYVFGGGNGY 437
           N +++ IGG+DG +HL+T   + PS    K +  M++ R   +VA +   +Y  GG +  
Sbjct: 429 NGNLYAIGGHDGTAHLATAEAFQPSIRQWKRIASMKTARRGIAVASIENVIYAVGGLDDT 488

Query: 438 AWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFAIGGGNGIESFSDVEMLDLDL 497
             Y TVE Y +E D WS+   ++V +G +G   IG  LFAIGG +G  S    E  D  +
Sbjct: 489 TCYKTVERYDIEEDEWSTVADMDVQRGGVGVAVIGRYLFAIGGNDGTSSLETCERFDPMI 548

Query: 498 GRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLYATGGFDGSDYLKSAERFDIR 557
            +W    SM  R              R       L+G LYA GGFD +  L++ ER+D  
Sbjct: 549 DKWKRIASMKNR--------------RAGSGVCVLDGYLYAIGGFDDNAPLETCERYDPD 608

Query: 558 EHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVSTVE 600
              W  +  M++ RG   + +L  K+YA+GG DG   ++TVE
Sbjct: 609 ADKWITLDKMSSPRGGVGVAALGGKVYAIGGHDGSDYLNTVE 636

BLAST of Spg033600 vs. ExPASy Swiss-Prot
Match: O94889 (Kelch-like protein 18 OS=Homo sapiens OX=9606 GN=KLHL18 PE=1 SV=3)

HSP 1 Score: 131.7 bits (330), Expect = 2.8e-29
Identity = 75/230 (32.61%), Postives = 116/230 (50.43%), Query Frame = 0

Query: 378 NESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRAYASVAWLNGQLYVFGGGNGY 437
           N  ++ IGGYDG+  LST+  Y+P  D    +  M S R+      L+GQ+YV GG +G 
Sbjct: 335 NGLLYAIGGYDGQLRLSTVEAYNPETDTWTRVGSMNSKRSAMGTVVLDGQIYVCGGYDGN 394

Query: 438 AWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFAIGGGNGIESFSDVEMLDLDL 497
           +   +VE+YS ETD+W+   S++  + + G      +++  GG +G++ FS VE  +   
Sbjct: 395 SSLSSVETYSPETDKWTVVTSMSSNRSAAGVTVFEGRIYVSGGHDGLQIFSSVEHYNHHT 454

Query: 498 GRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLYATGGFDGSDYLKSAERFDIR 557
             W     ML               KR    A  L   ++  GG+DGS +L  AE +   
Sbjct: 455 ATWHPAAGMLN--------------KRCRHGAASLGSKMFVCGGYDGSGFLSIAEMYSSV 514

Query: 558 EHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVSTVEVESYKEGC 608
              W  I  M+ +R   SLV+   +LYA+GG+DG S +S+VE+   +  C
Sbjct: 515 ADQWCLIVPMHTRRSRVSLVASCGRLYAVGGYDGQSNLSSVEMYDPETDC 550

BLAST of Spg033600 vs. ExPASy Swiss-Prot
Match: E9Q4F2 (Kelch-like protein 18 OS=Mus musculus OX=10090 GN=Klhl18 PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 6.3e-29
Identity = 74/223 (33.18%), Postives = 115/223 (51.57%), Query Frame = 0

Query: 378 NESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRAYASVAWLNGQLYVFGGGNGY 437
           N  ++ IGGYDG+  LST+  Y+P  D    +  M S R+      L+GQ+YV GG +G 
Sbjct: 335 NGLLYAIGGYDGQLRLSTVEAYNPETDTWTRVGSMNSKRSAMGTVVLDGQIYVCGGYDGN 394

Query: 438 AWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFAIGGGNGIESFSDVEMLDLDL 497
           +  ++VE+YS ETD+W+    ++  + + G      +++  GG +G++ FS VE  +   
Sbjct: 395 SSLNSVETYSPETDKWTVVTPMSSNRSAAGVTVFEGRIYVSGGHDGLQIFSSVEHYNHHT 454

Query: 498 GRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLYATGGFDGSDYLKSAERFDIR 557
             W    SML               KR    A  L   ++  GG+DGS +L  AE +   
Sbjct: 455 ATWHPAASMLN--------------KRCRHGAASLGSKMFVCGGYDGSGFLSIAEMYSSV 514

Query: 558 EHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVSTVEV 601
              W  I  M+ +R   SLV+   +LYA+GG+DG S +S+VE+
Sbjct: 515 ADQWCLIVPMHTRRSRVSLVASCGRLYAVGGYDGQSNLSSVEM 543

BLAST of Spg033600 vs. ExPASy TrEMBL
Match: A0A6J1J5H4 (influenza virus NS1A-binding protein homolog OS=Cucurbita maxima OX=3661 GN=LOC111481533 PE=4 SV=1)

HSP 1 Score: 968.4 bits (2502), Expect = 1.5e-278
Identity = 506/676 (74.85%), Postives = 550/676 (81.36%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNTG++ALKFKAGSSARL+   V RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLP+QHF
Sbjct: 1   MNTGQRALKFKAGSSARLDADEVYRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPSQHF 60

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYVKNID GLPLFLFNYSERKL+GIFEAASSGQ+NINPYGWTTDGSERTLYPAQVQILVR
Sbjct: 61  SYVKNIDPGLPLFLFNYSERKLNGIFEAASSGQININPYGWTTDGSERTLYPAQVQILVR 120

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
           K CQPLLENQFKP+I DNYY P+HFWFELDHAQTNKL+SLL+SQA+ PHVPQ TT  R  
Sbjct: 121 KLCQPLLENQFKPIIKDNYYSPNHFWFELDHAQTNKLISLLSSQAMTPHVPQFTTNCRPC 180

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C  LP LE+ DE EKIK QI+E+HF L SQV D VDV SSLDAGNS+ G H  ANE++EE
Sbjct: 181 CTVLPSLESRDEGEKIKSQIMEEHFDLESQVVDAVDVTSSLDAGNSSLGAHCYANESDEE 240

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
            K R+LHKLQQ AI HHESS+LP TSD NHTT N+  NL NN  SGEPIKS+ESNEED G
Sbjct: 241 LKCRILHKLQQFAIKHHESSMLPLTSDMNHTTINKGNNLVNNGSSGEPIKSKESNEEDFG 300

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMMS-YLPNSN 360
           +LT  QS IAKLVQE QELKDS AEQA++IV LEEKLLAAE EI ELK R+ S YLPNSN
Sbjct: 301 TLTGLQSPIAKLVQELQELKDSNAEQARRIVFLEEKLLAAEGEIQELKGRITSNYLPNSN 360

Query: 361 AVEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRA 420
           AVEA+R +VEEQ  D CLDP+ESIFLIGGYDG SHLSTL LYDPS D+IKSL PM SVRA
Sbjct: 361 AVEAKRVVVEEQLEDLCLDPDESIFLIGGYDGASHLSTLELYDPSSDMIKSLRPMSSVRA 420

Query: 421 YASVAWLNGQLYVFGGGNGYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFA 480
           YASVAWLN QLYVFGGGNG AWY+TVESY+LETD+W+ CPSLN+AKGSLGGV+IGNKLFA
Sbjct: 421 YASVAWLNSQLYVFGGGNGCAWYNTVESYNLETDQWTLCPSLNLAKGSLGGVSIGNKLFA 480

Query: 481 IGGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLY 540
           IGGGNGIESFS VEMLD++LGRWICTRSMLQR              RFA+AAVELNGVLY
Sbjct: 481 IGGGNGIESFSGVEMLDMELGRWICTRSMLQR--------------RFAVAAVELNGVLY 540

Query: 541 ATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVST 600
           ATGGFDG+DY++SAERFDIREHSWTQIPSMNAKRGCHSLV+LNEKLYALGGFDGDSMVS+
Sbjct: 541 ATGGFDGNDYMRSAERFDIREHSWTQIPSMNAKRGCHSLVTLNEKLYALGGFDGDSMVSS 600

Query: 601 VE----------------------------------------------VESYKEGCGWQE 628
           VE                                              VESYKEGCGWQE
Sbjct: 601 VEVYDPRMESWIDGEPMNKTRGYAAAGVINESIYIIGGVLVDDKILNTVESYKEGCGWQE 660

BLAST of Spg033600 vs. ExPASy TrEMBL
Match: A0A6J1F1G5 (influenza virus NS1A-binding protein homolog OS=Cucurbita moschata OX=3662 GN=LOC111441514 PE=4 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 2.3e-276
Identity = 502/677 (74.15%), Postives = 550/677 (81.24%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNTG++ALKFKAGSS+RL+ G V RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLP+QHF
Sbjct: 1   MNTGQRALKFKAGSSSRLDAGEVYRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPSQHF 60

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYVKNID GLPLFLFNYSERKL+GIFEAASSGQ+NINPYGWTTDGSERTLYPAQVQILVR
Sbjct: 61  SYVKNIDPGLPLFLFNYSERKLNGIFEAASSGQININPYGWTTDGSERTLYPAQVQILVR 120

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
           K CQPLLENQFKP+I DNYY P+HFWFELDHAQTNKL+SLL+SQA+  HVPQ TT  +  
Sbjct: 121 KLCQPLLENQFKPIIKDNYYSPNHFWFELDHAQTNKLISLLSSQAMTLHVPQFTTNCKPC 180

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C  LP LE+ DE EKIK QI+E+HF L SQV D VDV SSLDAGNSA G H  ANE++EE
Sbjct: 181 CTVLPSLESRDEGEKIKSQIMEEHFDLESQVVDAVDVTSSLDAGNSALGAHCYANESDEE 240

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
            K R+LHKLQQ AI HHES +LP TSD NHTT N+  NL NN  SGEPIKS+ES+EED G
Sbjct: 241 VKCRILHKLQQFAIKHHESPMLPLTSDMNHTTINKGNNLVNNGSSGEPIKSKESDEEDFG 300

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMMS-YLPNSN 360
           +LT  QS IAKLVQE QELK+S AEQA++IV LEEKLLAAE EI ELK R+ S  LPNSN
Sbjct: 301 TLTGLQSPIAKLVQELQELKESNAEQARRIVFLEEKLLAAEGEIQELKGRITSNNLPNSN 360

Query: 361 AVEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRA 420
           AVEA+R +VEEQ  D CLDP+ESIFLIGGYDG SHLSTL LYDPS D+IKSL PM SVRA
Sbjct: 361 AVEAKRVVVEEQLEDLCLDPDESIFLIGGYDGASHLSTLELYDPSSDMIKSLRPMSSVRA 420

Query: 421 YASVAWLNGQLYVFGGGNGYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFA 480
           YASVAWLN QLYVFGGGNG AWY+TVESY+LETD+W+ CPSLN+AKGSLGGV+IGNKLFA
Sbjct: 421 YASVAWLNSQLYVFGGGNGCAWYNTVESYNLETDQWTLCPSLNLAKGSLGGVSIGNKLFA 480

Query: 481 IGGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLY 540
           IGGGNGIESFSDVEMLD+DLGRWICTRSMLQR              RFA+AAVELNGVLY
Sbjct: 481 IGGGNGIESFSDVEMLDMDLGRWICTRSMLQR--------------RFAVAAVELNGVLY 540

Query: 541 ATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVST 600
           ATGGFDG+DY++SAERFDIREHSWTQIP+MNAKRGCHSLV+LNEKLYA+GGFDGDSMVS+
Sbjct: 541 ATGGFDGNDYMRSAERFDIREHSWTQIPNMNAKRGCHSLVTLNEKLYAVGGFDGDSMVSS 600

Query: 601 VE----------------------------------------------VESYKEGCGWQE 629
           VE                                              VESYKEGCGWQE
Sbjct: 601 VEVYDPRMESWIVGEPMNKTRGYAAAGVINESIYIIGGVLIDDKILNTVESYKEGCGWQE 660

BLAST of Spg033600 vs. ExPASy TrEMBL
Match: A0A6J1DU24 (influenza virus NS1A-binding protein homolog OS=Momordica charantia OX=3673 GN=LOC111024010 PE=4 SV=1)

HSP 1 Score: 948.3 bits (2450), Expect = 1.6e-272
Identity = 506/677 (74.74%), Postives = 541/677 (79.91%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNTG++ALKFK GSSA LN  A +RNLSKCHLGSVIFGCTNSTIKECLS QLFGLP QHF
Sbjct: 1   MNTGRRALKFKGGSSAPLNASAATRNLSKCHLGSVIFGCTNSTIKECLSKQLFGLPTQHF 60

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYVKNID GLPLFLFNYS RKLHGIFEAAS GQMNINPYGWTTDGSERTLYPAQVQI VR
Sbjct: 61  SYVKNIDPGLPLFLFNYSNRKLHGIFEAASYGQMNINPYGWTTDGSERTLYPAQVQIHVR 120

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
            +CQPLLENQFKP+ITDNYYGPSHFWFELDHAQTNKL+SLLASQAVA  VPQ TTKWR  
Sbjct: 121 MECQPLLENQFKPIITDNYYGPSHFWFELDHAQTNKLISLLASQAVASRVPQFTTKWRTF 180

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C GL  LETSD S+KIKP+ILE+HF L SQVSDTVDV SSLDAGN AF  H DAN  NEE
Sbjct: 181 CTGLSSLETSDGSDKIKPEILEEHFDLASQVSDTVDVNSSLDAGNRAFEAHFDANLANEE 240

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
           E+ R+  KLQQLA NHHESS LP TSDT  TT N+DTNLEN  +SGEPI+S+++NEED G
Sbjct: 241 EEYRIFLKLQQLATNHHESS-LPLTSDTECTTSNKDTNLENKGNSGEPIESKDNNEEDCG 300

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMM-SYLPNSN 360
           S TE QSL+AKLVQE QELKD KAEQA+KI  LE KLL AE EI ELKSRMM S L NSN
Sbjct: 301 SSTELQSLMAKLVQEIQELKDYKAEQAEKIAFLETKLLEAEGEIQELKSRMMSSSLSNSN 360

Query: 361 AVEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRA 420
           AVE ERA+ EEQ  D CLDPNESIFLIGGYDGES LSTL LYDP RD+IKSL PMR+VR+
Sbjct: 361 AVEPERAVGEEQFEDSCLDPNESIFLIGGYDGESWLSTLELYDPFRDVIKSLRPMRNVRS 420

Query: 421 YASVAWLNGQLYVFGGGNGY-AWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLF 480
           YASVAWLN QLYVFGGGNGY AWY+TVESY+LETD+W+  PSL+  KGSLGGV IGNKLF
Sbjct: 421 YASVAWLNSQLYVFGGGNGYSAWYNTVESYNLETDQWTLGPSLSAEKGSLGGVTIGNKLF 480

Query: 481 AIGGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVL 540
           AIGGGNGIESFSDVEMLDLDLGRWICTRSMLQR              RFA+AAVEL+G+L
Sbjct: 481 AIGGGNGIESFSDVEMLDLDLGRWICTRSMLQR--------------RFAVAAVELDGLL 540

Query: 541 YATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVS 600
           YATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLV+LN+KLY +GGFDGDSMVS
Sbjct: 541 YATGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVALNDKLYVIGGFDGDSMVS 600

Query: 601 TVEV----------------------------------------------ESYKEGCGWQ 628
           +VEV                                              ESYKEG GWQ
Sbjct: 601 SVEVYDPRMESWIIGEPMKNSRGYAAAGVINESIHVIGGVRLDGTIMDTAESYKEGRGWQ 660

BLAST of Spg033600 vs. ExPASy TrEMBL
Match: A0A0A0L9S8 (DCD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G730840 PE=4 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.7e-258
Identity = 481/675 (71.26%), Postives = 523/675 (77.48%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNT KKALKFK G +AR N  A  RNLSKCHLG VIFGCTNSTIKECLS QLFGLP+QHF
Sbjct: 1   MNTRKKALKFKPGPAARFNADASCRNLSKCHLGGVIFGCTNSTIKECLSKQLFGLPSQHF 60

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYV NID GLPLFLFNYSERKLHGIFEAASSGQMNIN YGWT DGSERT YPAQVQI+VR
Sbjct: 61  SYVMNIDPGLPLFLFNYSERKLHGIFEAASSGQMNINTYGWTADGSERTPYPAQVQIMVR 120

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
           +QCQPLLENQFKP+ITDNYYG +HFWFELDHAQTNKL+SLLASQA+AP V   TT  R  
Sbjct: 121 RQCQPLLENQFKPIITDNYYGLNHFWFELDHAQTNKLISLLASQAMAPSVRPSTTNHRPF 180

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C  LP LET + SEKIKPQ ++  + L SQV+DT+DV SSLDAGNSAF  H D NE NEE
Sbjct: 181 CTVLPSLETREGSEKIKPQNMDVQWDLASQVADTIDVTSSLDAGNSAFEVHCDENEVNEE 240

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
           EK RLLHKLQ+LA NHHES +LP TSDT+HT  N+D NLEN+    EPIKS+ES+ ED G
Sbjct: 241 EKNRLLHKLQELARNHHESPMLPLTSDTDHTALNKDKNLENSDRYVEPIKSKESSVEDFG 300

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMMSYLPNSNA 360
           S TEF SLIAKLVQE  EL++SKAEQ +KIVLLEEKLL AE EI+ELKS     L NSNA
Sbjct: 301 SSTEFPSLIAKLVQEIHELRESKAEQTEKIVLLEEKLLVAEGEIMELKS--FFTLQNSNA 360

Query: 361 VEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRAY 420
           +EA+R + EEQ  + CLDP ESIFLIGGYDG SHLSTL LYDPSRD+IKSL  MRSVR Y
Sbjct: 361 LEAKRVVEEEQIENSCLDPRESIFLIGGYDGASHLSTLELYDPSRDMIKSLRAMRSVRGY 420

Query: 421 ASVAWLNGQLYVFGGGNGYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFAI 480
           ASVAWLN QLYV GGGNG  WY+TVESY+LETD+W+  PSLN+ KGSLGGVNIG+KLFAI
Sbjct: 421 ASVAWLNSQLYVLGGGNGCVWYNTVESYNLETDQWTLSPSLNLEKGSLGGVNIGDKLFAI 480

Query: 481 GGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLYA 540
           GGGNGIES SDVEMLDL LGRWI TRSMLQR              RFA+ AVELNGVLYA
Sbjct: 481 GGGNGIESLSDVEMLDLYLGRWIRTRSMLQR--------------RFAVGAVELNGVLYA 540

Query: 541 TGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVSTV 600
           TGGFDGSDYLKSAERFDIREHSWTQI SMN KRGCHSLV+LN+KLYALGGFDG SMVS+V
Sbjct: 541 TGGFDGSDYLKSAERFDIREHSWTQIASMNEKRGCHSLVTLNDKLYALGGFDGRSMVSSV 600

Query: 601 E----------------------------------------------VESYKEGCGWQEK 628
           E                                              VE+YKEG GWQEK
Sbjct: 601 EVYDPRMESWIIGEPMKRMRGYAAAGVINESIYIIGGVLVDDKILDTVETYKEGYGWQEK 659

BLAST of Spg033600 vs. ExPASy TrEMBL
Match: A0A5A7TLJ1 (Kelch-like protein 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005610 PE=4 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 8.1e-253
Identity = 473/675 (70.07%), Postives = 517/675 (76.59%), Query Frame = 0

Query: 1   MNTGKKALKFKAGSSARLNVGAVSRNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHF 60
           MNTGKKALKFK   +AR N  A  RNL KCHLG VIFGCT STIKECLS Q+FGLP+QHF
Sbjct: 1   MNTGKKALKFKPEPAARYNAHASCRNLGKCHLGGVIFGCTKSTIKECLSKQMFGLPSQHF 60

Query: 61  SYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVR 120
           SYV NID GLPLFLFNYS+RKLHGIFEAASSGQMNIN YGWT DG+ERT YPAQVQI+VR
Sbjct: 61  SYVMNIDPGLPLFLFNYSDRKLHGIFEAASSGQMNINTYGWTADGAERTPYPAQVQIIVR 120

Query: 121 KQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAV 180
           + CQPLLENQFKP+I DNYYGP+ FWFELDHAQTNKL+SLLASQA+AP V   TT  R  
Sbjct: 121 RLCQPLLENQFKPIIIDNYYGPNRFWFELDHAQTNKLISLLASQAIAPSVRPSTTNRRPF 180

Query: 181 CAGLPYLETSDESEKIKPQILEDHFGLTSQVSDTVDVMSSLDAGNSAFGPHSDANETNEE 240
           C  LP LET D SEKIKPQI++    L SQ +DT+DV SSLDAGNSAFG H DANE NEE
Sbjct: 181 CTVLPSLETRDGSEKIKPQIMDVQCDLASQEADTIDVTSSLDAGNSAFGAHCDANEVNEE 240

Query: 241 EKRRLLHKLQQLAINHHESSLLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMG 300
           E   LLHKL+QLA N H S +LP TSDT+HT  N+D NLENN  S EPIKS+ES+ ED  
Sbjct: 241 ES-LLLHKLEQLARNLHASPMLPLTSDTDHTALNKDENLENNDRSVEPIKSKESSVEDFD 300

Query: 301 SLTEFQSLIAKLVQETQELKDSKAEQAKKIVLLEEKLLAAEEEILELKSRMMSYLPNSNA 360
           S TE   LIA+LVQE QEL++SKAEQ +KIV LEEKLL AE EILELKS +   LPNSNA
Sbjct: 301 SSTELPPLIAELVQEIQELRESKAEQTEKIVFLEEKLLVAEGEILELKSLLT--LPNSNA 360

Query: 361 VEAERAMVEEQ--DPCLDPNESIFLIGGYDGESHLSTLALYDPSRDLIKSLWPMRSVRAY 420
           +EA+R + EEQ  + CLDP ESIFLIGGYDG SHLSTL LYDPSRD+IKSL  MRSVR Y
Sbjct: 361 LEAKRVVEEEQIENSCLDPRESIFLIGGYDGASHLSTLELYDPSRDMIKSLRAMRSVRGY 420

Query: 421 ASVAWLNGQLYVFGGGNGYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFAI 480
           ASVAWLN QLYV GGGNG  WY+TVESY+LETD+W+  PSLN+AKGSLGGV+IGNKLFAI
Sbjct: 421 ASVAWLNSQLYVLGGGNGCVWYNTVESYNLETDQWTLSPSLNLAKGSLGGVSIGNKLFAI 480

Query: 481 GGGNGIESFSDVEMLDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLYA 540
           GGGNGIES S+VEMLDLDLGRWI TRSM QR              RFA+ AVELNG+LYA
Sbjct: 481 GGGNGIESLSEVEMLDLDLGRWIFTRSMRQR--------------RFAVGAVELNGILYA 540

Query: 541 TGGFDGSDYLKSAERFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVSTV 600
           TGGFDGSDYLKSAERFDIREHSWTQI SMN KRGCHSLV+LNEKLYALGGFDG SMVS+V
Sbjct: 541 TGGFDGSDYLKSAERFDIREHSWTQIASMNEKRGCHSLVTLNEKLYALGGFDGRSMVSSV 600

Query: 601 E----------------------------------------------VESYKEGCGWQEK 628
           E                                              VE+YKEG GWQEK
Sbjct: 601 EVYDPRMESWIFEEPMERTRGYAAAGVINESIYVIGGLFVDHKILDTVETYKEGYGWQEK 658

BLAST of Spg033600 vs. TAIR 10
Match: AT5G01660.1 (CONTAINS InterPro DOMAIN/s: Galactose oxidase/kelch, beta-propeller (InterPro:IPR011043), Kelch repeat type 1 (InterPro:IPR006652), Development/cell death domain (InterPro:IPR013989), Kelch related (InterPro:IPR013089), Kelch-type beta propeller (InterPro:IPR015915); BEST Arabidopsis thaliana protein match is: DCD (Development and Cell Death) domain protein (TAIR:AT3G11000.1); Has 16133 Blast hits to 7053 proteins in 482 species: Archae - 40; Bacteria - 1227; Metazoa - 10756; Fungi - 311; Plants - 1896; Viruses - 673; Other Eukaryotes - 1230 (source: NCBI BLink). )

HSP 1 Score: 491.5 bits (1264), Expect = 1.0e-138
Identity = 293/661 (44.33%), Postives = 381/661 (57.64%), Query Frame = 0

Query: 25  RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHFSYVKNIDSGLPLFLFNYSERKLHG 84
           RNL+K  LG V+FGCT +TIKEC+S QLFGLP+ H+ YV+ ID GLPLFLFNYS+R LHG
Sbjct: 11  RNLTKSQLGGVVFGCTKNTIKECMSKQLFGLPSNHYPYVQKIDIGLPLFLFNYSDRTLHG 70

Query: 85  IFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVRKQCQPLLENQFKPVITDNYYGPSH 144
           IFEAA  GQ+N +PYGWT+DGSERT YPAQV I VR QC+PL E +FKP I DNYY   H
Sbjct: 71  IFEAAGCGQLNFDPYGWTSDGSERTSYPAQVPISVRLQCEPLSEEKFKPAIADNYYSSHH 130

Query: 145 FWFELDHAQTNKLLSLLASQAVAPHVPQCTTKWRAVCAGLPYLETSDESEKIKPQILEDH 204
           FWFELDH QT KL  LL S AV P  P  T   R +   +   E  + S+++KP   E  
Sbjct: 131 FWFELDHFQTRKLTCLLTSFAVKPKPPMNTPNTRQIFRLISSSEKKENSDEVKPSENEPV 190

Query: 205 FGLTSQVSDTVDVMSSLDAGNSAFGP-HSDANETNEEEKRRLLHKLQQLAINHHE---SS 264
             L   +S   +  SS  A +  F   H D     + +K  +L KL+ L  +H E   +S
Sbjct: 191 GSLEVSLSSGGESDSSAAASHPGFSENHPDVQNPKQIDKDHVLEKLKDLVFSHDEHGDNS 250

Query: 265 LLPSTSDTNHTTHNEDTNLENNCHSGEPIKSEESNEEDMGSLTEFQSLIAKLVQETQELK 324
           L  +    N  T     NLE+     E   SE   +      +     I++L+ E +EL+
Sbjct: 251 LTETVEQANIPTCK---NLEDRDTLEEETCSEGKIDGSCLVSSPLPHTISQLMHEVKELR 310

Query: 325 DSKAEQAKKIVLLEEKLLAAEEEILELKSR--MMSYLPNSNAVEAERAMVE---EQDPCL 384
               E + KI  LEEKL  A +EI +L  R  M+  +      +A  + +E     D  L
Sbjct: 311 ACGLENSTKICYLEEKLDKAHKEIYQLTERCNMLESISGPLITKAGGSDLEIHSPDDTSL 370

Query: 385 DPNESIFLIGGY--DGESHLSTLALYDPSRDLIKSLWPMRSVRAYASVAWLNGQLYVFGG 444
           DP E+I L+GG+  D E+ LS++  Y PSR+++K+   M  +R+ ASVA L+G++YVFGG
Sbjct: 371 DPTEAILLLGGFDKDSETWLSSVQSYFPSRNVVKAHSSMSCIRSNASVAKLDGKIYVFGG 430

Query: 445 GN-GYAWYDTVESYSLETDRWSSCPSLNVAKGSLGGVNIGNKLFAIGGGNGIESFSDVEM 504
            + G  W ++ ES++    +WS CP LN  KGSLGG  +  K+FAIGGGNG+ SFSDVEM
Sbjct: 431 DDGGRGWTNSAESFNQTDGQWSLCPPLNERKGSLGGATLDGKIFAIGGGNGMVSFSDVEM 490

Query: 505 LDLDLGRWICTRSMLQRVNGVSSMFVMFNFKRFALAAVELNGVLYATGGFDGSDYLKSAE 564
           LD D+GRWI TRSM Q              +RFA+A+VE    +YA GG+DG +YL +AE
Sbjct: 491 LDPDIGRWIRTRSMGQ--------------ERFAVASVEHKSSIYAVGGYDGKEYLNTAE 550

Query: 565 RFDIREHSWTQIPSMNAKRGCHSLVSLNEKLYALGGFDGDSMVSTVE------------- 624
           RFD REHSW  I SM ++RGCHSLV LNEKLYA+GGFDG++MVS+VE             
Sbjct: 551 RFDPREHSWMNIASMKSRRGCHSLVVLNEKLYAIGGFDGETMVSSVEIYEPRTGTWMTGE 610

Query: 625 ----------------------------------VESYKEGCGWQEKTSRVLKKRCFQSA 627
                                             VE +KEG GW+   S  + +RCF SA
Sbjct: 611 PMKDLRGYSAVAVVKDSIYVIGGYKGEEDDILDTVECFKEGEGWKNVPSSSIGRRCFLSA 654

BLAST of Spg033600 vs. TAIR 10
Match: AT3G11000.1 (DCD (Development and Cell Death) domain protein )

HSP 1 Score: 176.8 bits (447), Expect = 5.5e-44
Identity = 85/145 (58.62%), Postives = 104/145 (71.72%), Query Frame = 0

Query: 25  RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHFSYVKNIDSGLPLFLFNYSERKLHG 84
           RNL K  L  VIFGC  STIKEC +  LFGLPA H +Y+KNID GL LFLFNYS+R LHG
Sbjct: 4   RNLKKGDLCGVIFGCKFSTIKECYAKNLFGLPAPHMAYIKNIDPGLTLFLFNYSDRTLHG 63

Query: 85  IFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVRKQCQPLLENQFKPVITDNYYGPSH 144
           IFEAAS G++NI+   W+ +G++ + YPAQV++ VR +C+PL E +F PVI +NY     
Sbjct: 64  IFEAASEGKLNIDSKAWSPNGTDPSPYPAQVKVRVRVRCEPLPEEKFSPVIVENYNDDKM 123

Query: 145 FWFELDHAQTNKLLSLL-ASQAVAP 169
           FWFELD  QTNKLL L   S +V P
Sbjct: 124 FWFELDRGQTNKLLRLFKPSPSVRP 148

BLAST of Spg033600 vs. TAIR 10
Match: AT3G11000.2 (DCD (Development and Cell Death) domain protein )

HSP 1 Score: 176.8 bits (447), Expect = 5.5e-44
Identity = 85/145 (58.62%), Postives = 104/145 (71.72%), Query Frame = 0

Query: 25  RNLSKCHLGSVIFGCTNSTIKECLSNQLFGLPAQHFSYVKNIDSGLPLFLFNYSERKLHG 84
           RNL K  L  VIFGC  STIKEC +  LFGLPA H +Y+KNID GL LFLFNYS+R LHG
Sbjct: 123 RNLKKGDLCGVIFGCKFSTIKECYAKNLFGLPAPHMAYIKNIDPGLTLFLFNYSDRTLHG 182

Query: 85  IFEAASSGQMNINPYGWTTDGSERTLYPAQVQILVRKQCQPLLENQFKPVITDNYYGPSH 144
           IFEAAS G++NI+   W+ +G++ + YPAQV++ VR +C+PL E +F PVI +NY     
Sbjct: 183 IFEAASEGKLNIDSKAWSPNGTDPSPYPAQVKVRVRVRCEPLPEEKFSPVIVENYNDDKM 242

Query: 145 FWFELDHAQTNKLLSLL-ASQAVAP 169
           FWFELD  QTNKLL L   S +V P
Sbjct: 243 FWFELDRGQTNKLLRLFKPSPSVRP 267

BLAST of Spg033600 vs. TAIR 10
Match: AT2G35140.1 (DCD (Development and Cell Death) domain protein )

HSP 1 Score: 117.1 bits (292), Expect = 5.2e-26
Identity = 56/131 (42.75%), Postives = 84/131 (64.12%), Query Frame = 0

Query: 36  IFGCTNSTIKECLSNQLFGLPAQHFSYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMN 95
           IF   NST KECLS +LFGLP     +VK++ +G+ LFLF + +R+LHG+F+A S G +N
Sbjct: 25  IFMSNNSTRKECLSRKLFGLPIGLGGFVKHVKAGMMLFLFEFEKRELHGVFQACSDGAIN 84

Query: 96  INPYGWTTDGSERTLYPAQVQILVRKQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTN 155
           I P  + + G +   +PAQV+   + +C+PL E++F   I +NY+ P+ F F L  AQ  
Sbjct: 85  IEPNAFRSSGKQ---FPAQVKFTEKWRCRPLCESEFGNAIHENYFTPTKFNFGLSKAQVQ 144

Query: 156 KLLSLLASQAV 167
           +LL L + + V
Sbjct: 145 RLLKLFSMKKV 152

BLAST of Spg033600 vs. TAIR 10
Match: AT2G35140.2 (DCD (Development and Cell Death) domain protein )

HSP 1 Score: 117.1 bits (292), Expect = 5.2e-26
Identity = 56/131 (42.75%), Postives = 84/131 (64.12%), Query Frame = 0

Query: 36  IFGCTNSTIKECLSNQLFGLPAQHFSYVKNIDSGLPLFLFNYSERKLHGIFEAASSGQMN 95
           IF   NST KECLS +LFGLP     +VK++ +G+ LFLF + +R+LHG+F+A S G +N
Sbjct: 25  IFMSNNSTRKECLSRKLFGLPIGLGGFVKHVKAGMMLFLFEFEKRELHGVFQACSDGAIN 84

Query: 96  INPYGWTTDGSERTLYPAQVQILVRKQCQPLLENQFKPVITDNYYGPSHFWFELDHAQTN 155
           I P  + + G +   +PAQV+   + +C+PL E++F   I +NY+ P+ F F L  AQ  
Sbjct: 85  IEPNAFRSSGKQ---FPAQVKFTEKWRCRPLCESEFGNAIHENYFTPTKFNFGLSKAQVQ 144

Query: 156 KLLSLLASQAV 167
           +LL L + + V
Sbjct: 145 RLLKLFSMKKV 152