Spg033543 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg033543
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionshort-chain dehydrogenase reductase 2a-like
Locationscaffold5: 726434 .. 728045 (-)
RNA-Seq ExpressionSpg033543
SyntenySpg033543
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAATATATTGTGGTTAGGAAGATTTAGATTGAATAAGAAGAAGACAAAGGGAAGGGAAGGGGGGTGGTTTTGTGGGGGGGCAAGCGAAACAGCACTAAGAAAGGAAGCTGCCTTGAAGTTGAAGCCCTCAAACACAAAACACAAAACACAACACACACACTCTCTTACACCAACTTCACAATTTCCTCTCCCTATAAATTCCCTTCCATTTCTCTCCTCTCTCACTTCCTCCTATACGCTTCTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTTCTCTCTCTCTCTAGCCATGACTGCCCAAGTGCTTTCCGAGCAAGCGCTTCAGAGCAATATTCACCTCTTGCAAAGGGACAATCCCACTCCACATAGAAGGTAATATTTTCACTCCATTTTGCCCCTTTATTCATCAACTTGGCATAAAGTTGTACTAATAACCAAGAAGTCTGGTTAAAAAACAAAAAATTAACCAAGAAGTCTGGTTAAAAAACAAAAAATTAACCGGTGGGATTAATTAACTATTTTGTTTAAAAATTTAAAGCGTAATTAATTATGTAGTGTGTATTATTGTTGATTTTAGGTTGGAGGGAAAAGTGGCGATCGTAACGGGCGGGGCAAAGGGAATCGGAGAAGCGACAGTGAGACTGTTCGCCAAACACGGAGCAAAAGTAGTAATCGCCGATGTGGAGGATCTCCTCGGCGAGGCTTTGGCCGACACGCTTTCGCCGTGTCCGGTGTCGTTCGTCCACTGCGACGTCAGCTGCGAGGAAGACGTGGAGAATCTAATCAACTCGACGATTCGTCGGCACGGCCAAGTCGACATAATATTCAACAACGCCGGAGTTTTGGGCAAACAGTCCAAGACCCACAAGAGCATCGTTGACTTTGACCCCGACGAGTTCGAGCGCGTGATGCGCGTGAACGTCAAAGGCGTCGCCTTGGGTATAAAGCACGCGGCGCGCGTGATGATTCCCAGAGCCACCGGCTGCATAATCTCCACTGCCAGCGTGGCAGGCGTTTTGGGGGGCATGGGCCCACACGCCTACACGGCATCTAAACACGCCATTGTCGGCCTCACAAAGAACACCGCCTGTGAGCTCGGCCCTTACGGCATACGTGTCAACTGTATTTCGCCGTTCGGAGTTGCCACGTCGATGCTGGTGAACGCCTGGAGGGCTGACGGGGACGAGGATGAGGACGAGTGCATGAACTTCGGGATCCCGTCGGCTGCTGAGGTGGACAAAATGGAGGAGTTCGTTAGGGGTTTGGCCAATCTGAAGGGCCCCACATTGAGGCCTAAGGATATTGCTGAGGCGGCGCTTTATCTCGCCAGCGATGAATCTAAATACATCAGTGGCCATAATCTCGTCGTCGATGGTGGAATCACCACTTCAAGAAATTGTGTTGGCTTGTAATTTTTATTTTTATTTTTCTTTTTATTTTTTATATTTTTTTTTCCAGGTATTATTGGTGGAAAAGAAAAAGAATTGACACTGAAATGCCTGTTGGTTGTACTTTGTAGTAGGATTAATTTAATTTTTATTTTTTTAATTGTTGTTTGTTCTATAATTCGAAGTACCAATAGCTGAATATGAATATATATTAGTTCCTTC

mRNA sequence

ATGACTGCCCAAGTGCTTTCCGAGCAAGCGCTTCAGAGCAATATTCACCTCTTGCAAAGGGACAATCCCACTCCACATAGAAGGTTGGAGGGAAAAGTGGCGATCGTAACGGGCGGGGCAAAGGGAATCGGAGAAGCGACAGTGAGACTGTTCGCCAAACACGGAGCAAAAGTAGTAATCGCCGATGTGGAGGATCTCCTCGGCGAGGCTTTGGCCGACACGCTTTCGCCGTGTCCGGTGTCGTTCGTCCACTGCGACGTCAGCTGCGAGGAAGACGTGGAGAATCTAATCAACTCGACGATTCGTCGGCACGGCCAAGTCGACATAATATTCAACAACGCCGGAGTTTTGGGCAAACAGTCCAAGACCCACAAGAGCATCGTTGACTTTGACCCCGACGAGTTCGAGCGCGTGATGCGCGTGAACGTCAAAGGCGTCGCCTTGGGTATAAAGCACGCGGCGCGCGTGATGATTCCCAGAGCCACCGGCTGCATAATCTCCACTGCCAGCGTGGCAGGCGTTTTGGGGGGCATGGGCCCACACGCCTACACGGCATCTAAACACGCCATTGTCGGCCTCACAAAGAACACCGCCTGTGAGCTCGGCCCTTACGGCATACGTGTCAACTGTATTTCGCCGTTCGGAGTTGCCACGTCGATGCTGGTGAACGCCTGGAGGGCTGACGGGGACGAGGATGAGGACGAGTGCATGAACTTCGGGATCCCGTCGGCTGCTGAGGTGGACAAAATGGAGGAGTTCGTTAGGGGTTTGGCCAATCTGAAGGGCCCCACATTGAGGCCTAAGGATATTGCTGAGGCGGCGCTTTATCTCGCCAGCGATGAATCTAAATACATCAGTGGCCATAATCTCGTCGTCGATGGTGGAATCACCACTTCAAGAAATTGTGTTGGCTTGTAA

Coding sequence (CDS)

ATGACTGCCCAAGTGCTTTCCGAGCAAGCGCTTCAGAGCAATATTCACCTCTTGCAAAGGGACAATCCCACTCCACATAGAAGGTTGGAGGGAAAAGTGGCGATCGTAACGGGCGGGGCAAAGGGAATCGGAGAAGCGACAGTGAGACTGTTCGCCAAACACGGAGCAAAAGTAGTAATCGCCGATGTGGAGGATCTCCTCGGCGAGGCTTTGGCCGACACGCTTTCGCCGTGTCCGGTGTCGTTCGTCCACTGCGACGTCAGCTGCGAGGAAGACGTGGAGAATCTAATCAACTCGACGATTCGTCGGCACGGCCAAGTCGACATAATATTCAACAACGCCGGAGTTTTGGGCAAACAGTCCAAGACCCACAAGAGCATCGTTGACTTTGACCCCGACGAGTTCGAGCGCGTGATGCGCGTGAACGTCAAAGGCGTCGCCTTGGGTATAAAGCACGCGGCGCGCGTGATGATTCCCAGAGCCACCGGCTGCATAATCTCCACTGCCAGCGTGGCAGGCGTTTTGGGGGGCATGGGCCCACACGCCTACACGGCATCTAAACACGCCATTGTCGGCCTCACAAAGAACACCGCCTGTGAGCTCGGCCCTTACGGCATACGTGTCAACTGTATTTCGCCGTTCGGAGTTGCCACGTCGATGCTGGTGAACGCCTGGAGGGCTGACGGGGACGAGGATGAGGACGAGTGCATGAACTTCGGGATCCCGTCGGCTGCTGAGGTGGACAAAATGGAGGAGTTCGTTAGGGGTTTGGCCAATCTGAAGGGCCCCACATTGAGGCCTAAGGATATTGCTGAGGCGGCGCTTTATCTCGCCAGCGATGAATCTAAATACATCAGTGGCCATAATCTCGTCGTCGATGGTGGAATCACCACTTCAAGAAATTGTGTTGGCTTGTAA

Protein sequence

MTAQVLSEQALQSNIHLLQRDNPTPHRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMGPHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVGL
Homology
[Close]
BLAST of Spg033543 vs. NCBI nr
Match: XP_022934318.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 559.3 bits (1440), Expect = 2.0e-155
Identity = 285/306 (93.14%), Postives = 294/306 (96.08%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDN-PTPHRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVL EQALQSNIHLL RDN P+ H+RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGK 120
           IADVEDLLG+ALADTLSP PVSFVHCDVS EEDVENLI+ST+ RHGQVDIIFNNAGVLG 
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 121 QSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMG 180
           QSKTHKSIVDFDP+EFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG+G
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNF 240
           PHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWRADGDE EDECMN 
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDE-EDECMNL 240

Query: 241 GIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300
           GIPS AEVDKMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS
Sbjct: 241 GIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300

Query: 301 RNCVGL 306
           RNC+GL
Sbjct: 301 RNCIGL 305

[Close]
BLAST of Spg033543 vs. NCBI nr
Match: XP_023528551.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 557.8 bits (1436), Expect = 6.0e-155
Identity = 284/306 (92.81%), Postives = 293/306 (95.75%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDN-PTPHRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVL EQALQSNIHLL RDN P+ H+RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 93  MTAQVLPEQALQSNIHLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 152

Query: 61  IADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGK 120
           IADVEDLLG+ALADTLSP PVSFVHCDVS EEDVENLI+ST+ RHGQVDIIFNNAGVLG 
Sbjct: 153 IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 212

Query: 121 QSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMG 180
           QSKTHKSIVDFDP+EFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG+G
Sbjct: 213 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 272

Query: 181 PHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNF 240
           PHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWRADGDE EDECMN 
Sbjct: 273 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDE-EDECMNL 332

Query: 241 GIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300
           GIPS  EVDKMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS
Sbjct: 333 GIPSVGEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 392

Query: 301 RNCVGL 306
           RNC+GL
Sbjct: 393 RNCIGL 397

[Close]
BLAST of Spg033543 vs. NCBI nr
Match: XP_022983300.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita maxima])

HSP 1 Score: 553.9 bits (1426), Expect = 8.6e-154
Identity = 283/306 (92.48%), Postives = 293/306 (95.75%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDN-PTPHRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVL EQALQ+NI LL RDN P+ H+RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQTNILLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGK 120
           IADVEDLLG+ALADTLSP PVSFVHCDVS EEDVENLI+ST+ RHGQVDIIFNNAGVLG 
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 121 QSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMG 180
           QSKTHKSIVDFDP+EFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG+G
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNF 240
           PHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWRADGDE EDECMN 
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDE-EDECMNL 240

Query: 241 GIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300
           GIPS AEVDKMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS
Sbjct: 241 GIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300

Query: 301 RNCVGL 306
           RNC+GL
Sbjct: 301 RNCIGL 305

[Close]
BLAST of Spg033543 vs. NCBI nr
Match: XP_022971590.1 (short-chain dehydrogenase reductase 2a-like [Cucurbita maxima])

HSP 1 Score: 549.7 bits (1415), Expect = 1.6e-152
Identity = 281/306 (91.83%), Postives = 290/306 (94.77%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDNPTP-HRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVL EQALQSNIHLLQRD+PTP ++RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGK 120
           IADVEDLLGEALADTLSP PV+FVHCDVS EED+ENLINST+ RHGQVDI+FNNAGVLG 
Sbjct: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 121 QSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMG 180
           QSKT KSIVDFDPDEFERVM VNVKGVALGIKHAARVMIPRATGCIISTASVAGV GGMG
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNF 240
           PHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWR DG    DECMNF
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG----DECMNF 240

Query: 241 GIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300
           GIPS AEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTS
Sbjct: 241 GIPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTS 300

Query: 301 RNCVGL 306
           RNC+GL
Sbjct: 301 RNCIGL 302

[Close]
BLAST of Spg033543 vs. NCBI nr
Match: XP_022947494.1 (short-chain dehydrogenase reductase 2a [Cucurbita moschata] >KAG6596319.1 Short-chain dehydrogenase reductase 2a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 548.5 bits (1412), Expect = 3.6e-152
Identity = 281/306 (91.83%), Postives = 288/306 (94.12%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDNPTP-HRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVL EQALQSNIHLLQRD P P ++RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDTPAPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGK 120
           IADVEDLLGEALADTLSP PVSFVHCDVS EED+ENLINST+ RHGQVDI+FNNAGVLG 
Sbjct: 61  IADVEDLLGEALADTLSPNPVSFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 121 QSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMG 180
           QSKT KSIVDFDPDEFERVM VNVKGVALGIKHAARVMIPRATGCIISTASVAGV GGMG
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNF 240
           PHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWR DG    DECMNF
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG----DECMNF 240

Query: 241 GIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300
           GIPS AEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTS
Sbjct: 241 GIPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTS 300

Query: 301 RNCVGL 306
           RNC+GL
Sbjct: 301 RNCIGL 302

[Close]
BLAST of Spg033543 vs. ExPASy Swiss-Prot
Match: Q9SCU0 (Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a PE=3 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 3.6e-110
Identity = 212/315 (67.30%), Postives = 252/315 (80.00%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIH--LLQRDNPTPH-RRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           M AQV++EQ    ++H  +++  N T + +RLEGKVAI+TGGA GIG+ATV LFA+HGA 
Sbjct: 1   MPAQVIAEQTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGAT 60

Query: 61  VVIADVEDLLGEALADTLSPCP----VSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNN 120
           VVIADV+++ G +LA +LS       V+F+ CDVS E DVENL+N T+ R+G++DI+FNN
Sbjct: 61  VVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNN 120

Query: 121 AGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRA-TGCIISTASVA 180
           AGVLG Q K HKSI+DFD DEF+ VMRVNV+GV LG+KH AR MI R   GCIISTASVA
Sbjct: 121 AGVLGDQKK-HKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVLGGMGPHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWR--ADGD 240
           GV+GGMGPHAYTASKHAIVGLTKN ACELG YGIRVNCISPFGVATSMLVNAWR  + GD
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGD 240

Query: 241 EDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNL 300
            ++D           +V++MEEFVR LANLKG TLR  DIAEAALYLASDESKY++GHNL
Sbjct: 241 VEDD-----------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNL 300

Query: 301 VVDGGITTSRNCVGL 306
           VVDGG+TT+RNCVGL
Sbjct: 301 VVDGGVTTARNCVGL 303

[Close]
BLAST of Spg033543 vs. ExPASy Swiss-Prot
Match: P50160 (Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 3.4e-105
Identity = 195/292 (66.78%), Postives = 232/292 (79.45%), Query Frame = 0 

Query: 23  PTP-HRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPCPVS 82
           PTP  +RL+GKVAIVTGGA+GIGEA VRLFAKHGA+VVIAD++D  GEALA  L P  VS
Sbjct: 46  PTPMPKRLDGKVAIVTGGARGIGEAIVRLFAKHGARVVIADIDDAAGEALASALGP-QVS 105

Query: 83  FVHCDVSCEEDVENLINSTIRRH-GQVDIIFNNAGVLGKQSKTHKSIVDFDPDEFERVMR 142
           FV CDVS E+DV   ++  + RH G++D+  NNAGVLG+Q++  +SI+ FD  EF+RV+R
Sbjct: 106 FVRCDVSVEDDVRRAVDWALSRHGGRLDVYCNNAGVLGRQTRAARSILSFDAAEFDRVLR 165

Query: 143 VNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMGPHAYTASKHAIVGLTKNTACE 202
           VN  G ALG+KHAAR M PR  G I+S ASVA VLGG+GPHAYTASKHAIVGLTKN ACE
Sbjct: 166 VNALGAALGMKHAARAMAPRRAGSIVSVASVAAVLGGLGPHAYTASKHAIVGLTKNAACE 225

Query: 203 LGPYGIRVNCISPFGVATSMLVNAWR-------ADGDEDEDECMNFGIPSAAEVDKMEEF 262
           L  +G+RVNC+SPFGVAT ML+NAWR       AD D D D  ++  +PS  EV+KMEE 
Sbjct: 226 LRAHGVRVNCVSPFGVATPMLINAWRQGHDDATADADRDLDLDLDVTVPSDQEVEKMEEV 285

Query: 263 VRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCVGL 306
           VRGLA LKGPTLRP+DIAEA L+LASDE++YISGHNLVVDGG+TTSRN +GL
Sbjct: 286 VRGLATLKGPTLRPRDIAEAVLFLASDEARYISGHNLVVDGGVTTSRNLIGL 336

[Close]
BLAST of Spg033543 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 6.8e-69
Identity = 132/277 (47.65%), Postives = 187/277 (67.51%), Query Frame = 0 

Query: 27  RRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPCPVSFVHCD 86
           RRLEGKVA++TGGA GIGE T +LF++HGAKV IADV+D LG ++ + +     +++HCD
Sbjct: 13  RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSVVEAIGTSNSTYIHCD 72

Query: 87  VSCEEDVENLINSTIRRHGQVDIIFNNAGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGV 146
           V+ E+ V+N +++T+  +G++DI+F+NAG+          I+D +  +FERV  VNV GV
Sbjct: 73  VTNEDGVKNAVDNTVSTYGKLDIMFSNAGI---SDPNRPRIIDNEKADFERVFSVNVTGV 132

Query: 147 ALGIKHAARVMIPRATGCIISTASVAGVLGGMGPHAYTASKHAIVGLTKNTACELGPYGI 206
            L +KHAARVMIP  +G IISTAS++  +GG   HAY  SKHA++GLT+N A ELG +GI
Sbjct: 133 FLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLAVELGQFGI 192

Query: 207 RVNCISPFGVATSMLVNAWRADGDEDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTLR 266
           RVNC+SPFG+ T++     +  G ++E+E               E  +    NLKGP   
Sbjct: 193 RVNCLSPFGLPTAL---GKKFSGIKNEEE--------------FENVINFAGNLKGPKFN 252

Query: 267 PKDIAEAALYLASDESKYISGHNLVVDGGITTSRNCV 304
            +D+A AALYLASDE+KY+SGHNL +DGG +   + +
Sbjct: 253 VEDVANAALYLASDEAKYVSGHNLFIDGGFSVCNSVI 269

[Close]
BLAST of Spg033543 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 9.8e-68
Identity = 135/275 (49.09%), Postives = 183/275 (66.55%), Query Frame = 0 

Query: 28  RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPCP-VSFVHCD 87
           RLEGKVA+VTGGA GIGE+  RLF +HGAK+ I DV+D LG+ ++  L   P   + HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 88  VSCEEDVENLINSTIRRHGQVDIIFNNAGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGV 147
           V+ E+DV   ++ T  ++G +DI+ NNAG+ G +      I D D +EF++V  +NV GV
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKV---IDIRDADFNEFKKVFDINVNGV 121

Query: 148 ALGIKHAARVMIPRATGCIISTASVAGVLGGMGPHAYTASKHAIVGLTKNTACELGPYGI 207
            LG+KHAAR+MIP+  G I+S ASV+ V+ G GPH YT +KHA+VGLTK+ A ELG +GI
Sbjct: 122 FLGMKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGI 181

Query: 208 RVNCISPFGVATSMLVNAWRADGDEDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTLR 267
           RVNC+SP+ V T + +  +  + +  ED    F             FVR  ANLKG  L 
Sbjct: 182 RVNCVSPYAVPTRLSM-PYLPESEMQEDALRGF-----------LTFVRSNANLKGVDLM 241

Query: 268 PKDIAEAALYLASDESKYISGHNLVVDGGITTSRN 302
           P D+AEA LYLA++ESKY+SG NLV+DGG + + +
Sbjct: 242 PNDVAEAVLYLATEESKYVSGLNLVIDGGFSIANH 261

[Close]
BLAST of Spg033543 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.2e-66
Identity = 137/272 (50.37%), Postives = 184/272 (67.65%), Query Frame = 0 

Query: 28  RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTL-SPCPVSFVHCD 87
           RL+ KVAI+TGGA GIGE T +LF ++GAKVVIAD+ D  G+ + + + SP  +SFVHCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 88  VSCEEDVENLINSTIRRHGQVDIIFNNAGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGV 147
           V+ +EDV NL+++TI +HG++DI+F N GVL   S T  SI++   ++F+RVM +NV G 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVL---STTPYSILEAGNEDFKRVMDINVYGA 132

Query: 148 ALGIKHAARVMIPRATGCIISTASVAGVLGGMG-PHAYTASKHAIVGLTKNTACELGPYG 207
            L  KHAARVMIP   G I+ TAS++    G G  H YTA+KHA++GLT +   ELG YG
Sbjct: 133 FLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYG 192

Query: 208 IRVNCISPFGVATSMLVNAWRADGDEDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTL 267
           IRVNC+SP+ VA+ +L +               FG+ S+    ++EE     ANLKG  L
Sbjct: 193 IRVNCVSPYIVASPLLTDV--------------FGVDSS----RVEELAHQAANLKGTLL 252

Query: 268 RPKDIAEAALYLASDESKYISGHNLVVDGGIT 298
           R +D+A+A  YLA DESKY+SG NLV+DGG T
Sbjct: 253 RAEDVADAVAYLAGDESKYVSGLNLVIDGGYT 263

[Close]
BLAST of Spg033543 vs. ExPASy TrEMBL
Match: A0A6J1F7B6 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC111441518 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 9.9e-156
Identity = 285/306 (93.14%), Postives = 294/306 (96.08%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDN-PTPHRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVL EQALQSNIHLL RDN P+ H+RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGK 120
           IADVEDLLG+ALADTLSP PVSFVHCDVS EEDVENLI+ST+ RHGQVDIIFNNAGVLG 
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 121 QSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMG 180
           QSKTHKSIVDFDP+EFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG+G
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNF 240
           PHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWRADGDE EDECMN 
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDE-EDECMNL 240

Query: 241 GIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300
           GIPS AEVDKMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS
Sbjct: 241 GIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300

Query: 301 RNCVGL 306
           RNC+GL
Sbjct: 301 RNCIGL 305

[Close]
BLAST of Spg033543 vs. ExPASy TrEMBL
Match: A0A6J1J5H2 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111481921 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.2e-154
Identity = 283/306 (92.48%), Postives = 293/306 (95.75%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDN-PTPHRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVL EQALQ+NI LL RDN P+ H+RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQTNILLLGRDNSPSLHKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGK 120
           IADVEDLLG+ALADTLSP PVSFVHCDVS EEDVENLI+ST+ RHGQVDIIFNNAGVLG 
Sbjct: 61  IADVEDLLGQALADTLSPSPVSFVHCDVSSEEDVENLISSTVCRHGQVDIIFNNAGVLGN 120

Query: 121 QSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMG 180
           QSKTHKSIVDFDP+EFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGG+G
Sbjct: 121 QSKTHKSIVDFDPNEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGLG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNF 240
           PHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWRADGDE EDECMN 
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRADGDE-EDECMNL 240

Query: 241 GIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300
           GIPS AEVDKMEEFVRGL+NLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS
Sbjct: 241 GIPSVAEVDKMEEFVRGLSNLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300

Query: 301 RNCVGL 306
           RNC+GL
Sbjct: 301 RNCIGL 305

[Close]
BLAST of Spg033543 vs. ExPASy TrEMBL
Match: A0A6J1I659 (short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC111470263 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 7.9e-153
Identity = 281/306 (91.83%), Postives = 290/306 (94.77%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDNPTP-HRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVL EQALQSNIHLLQRD+PTP ++RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDSPTPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGK 120
           IADVEDLLGEALADTLSP PV+FVHCDVS EED+ENLINST+ RHGQVDI+FNNAGVLG 
Sbjct: 61  IADVEDLLGEALADTLSPNPVTFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 121 QSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMG 180
           QSKT KSIVDFDPDEFERVM VNVKGVALGIKHAARVMIPRATGCIISTASVAGV GGMG
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNF 240
           PHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWR DG    DECMNF
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG----DECMNF 240

Query: 241 GIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300
           GIPS AEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTS
Sbjct: 241 GIPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTS 300

Query: 301 RNCVGL 306
           RNC+GL
Sbjct: 301 RNCIGL 302

[Close]
BLAST of Spg033543 vs. ExPASy TrEMBL
Match: A0A6J1G6Z3 (short-chain dehydrogenase reductase 2a OS=Cucurbita moschata OX=3662 GN=LOC111451343 PE=4 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.7e-152
Identity = 281/306 (91.83%), Postives = 288/306 (94.12%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDNPTP-HRRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60
           MTAQVL EQALQSNIHLLQRD P P ++RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV
Sbjct: 1   MTAQVLPEQALQSNIHLLQRDTPAPSYKRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVV 60

Query: 61  IADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGVLGK 120
           IADVEDLLGEALADTLSP PVSFVHCDVS EED+ENLINST+ RHGQVDI+FNNAGVLG 
Sbjct: 61  IADVEDLLGEALADTLSPNPVSFVHCDVSLEEDMENLINSTMCRHGQVDILFNNAGVLGN 120

Query: 121 QSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLGGMG 180
           QSKT KSIVDFDPDEFERVM VNVKGVALGIKHAARVMIPRATGCIISTASVAGV GGMG
Sbjct: 121 QSKTRKSIVDFDPDEFERVMCVNVKGVALGIKHAARVMIPRATGCIISTASVAGVSGGMG 180

Query: 181 PHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDECMNF 240
           PHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWR DG    DECMNF
Sbjct: 181 PHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRDDG----DECMNF 240

Query: 241 GIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGITTS 300
           GIPS AEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKY+SGHNLVVDGGITTS
Sbjct: 241 GIPSPAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYVSGHNLVVDGGITTS 300

Query: 301 RNCVGL 306
           RNC+GL
Sbjct: 301 RNCIGL 302

[Close]
BLAST of Spg033543 vs. ExPASy TrEMBL
Match: A0A0A0LF57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.1e-149
Identity = 271/309 (87.70%), Postives = 292/309 (94.50%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIHLLQRDN----PTPHRRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60
           MTAQVL EQ+LQSNIHLL RDN    P  H+RLEGKVAIVTGGAKGIGEATVRLFAKHGA
Sbjct: 1   MTAQVLPEQSLQSNIHLLVRDNSSSPPPFHKRLEGKVAIVTGGAKGIGEATVRLFAKHGA 60

Query: 61  KVVIADVEDLLGEALADTLSPCPVSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNNAGV 120
           KVVIADVED+LG+ALA+TLSP PVSFVHCDVS E+DVENL+++T+  HGQVDIIFNNAGV
Sbjct: 61  KVVIADVEDILGQALANTLSPSPVSFVHCDVSSEDDVENLVSTTVCLHGQVDIIFNNAGV 120

Query: 121 LGKQSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180
           LG QS +HKSI+DFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG
Sbjct: 121 LGNQSNSHKSILDFDPDEFERVMRVNVKGVALGIKHAARVMIPRATGCIISTASVAGVLG 180

Query: 181 GMGPHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWRADGDEDEDEC 240
           G+GPHAYTASKHAIVGLTKNTACELG YGIRVNCISPFGVATSMLVNAWRAD  E+E+EC
Sbjct: 181 GLGPHAYTASKHAIVGLTKNTACELGRYGIRVNCISPFGVATSMLVNAWRAD-VEEEEEC 240

Query: 241 MNFGIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNLVVDGGI 300
           MN+G+PSAAEVDKMEEFVRGLANLKGPTL+PKDIA+AALYLASDESKYISGHNLVVDGG+
Sbjct: 241 MNYGVPSAAEVDKMEEFVRGLANLKGPTLKPKDIAQAALYLASDESKYISGHNLVVDGGV 300

Query: 301 TTSRNCVGL 306
           TTSRNC+GL
Sbjct: 301 TTSRNCIGL 308

[Close]
BLAST of Spg033543 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 399.4 bits (1025), Expect = 2.5e-111
Identity = 212/315 (67.30%), Postives = 252/315 (80.00%), Query Frame = 0 

Query: 1   MTAQVLSEQALQSNIH--LLQRDNPTPH-RRLEGKVAIVTGGAKGIGEATVRLFAKHGAK 60
           M AQV++EQ    ++H  +++  N T + +RLEGKVAI+TGGA GIG+ATV LFA+HGA 
Sbjct: 1   MPAQVIAEQTTFHSVHDTIMEETNTTLYPKRLEGKVAIITGGAHGIGKATVMLFARHGAT 60

Query: 61  VVIADVEDLLGEALADTLSPCP----VSFVHCDVSCEEDVENLINSTIRRHGQVDIIFNN 120
           VVIADV+++ G +LA +LS       V+F+ CDVS E DVENL+N T+ R+G++DI+FNN
Sbjct: 61  VVIADVDNVAGSSLAKSLSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNN 120

Query: 121 AGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGVALGIKHAARVMIPRA-TGCIISTASVA 180
           AGVLG Q K HKSI+DFD DEF+ VMRVNV+GV LG+KH AR MI R   GCIISTASVA
Sbjct: 121 AGVLGDQKK-HKSILDFDADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVA 180

Query: 181 GVLGGMGPHAYTASKHAIVGLTKNTACELGPYGIRVNCISPFGVATSMLVNAWR--ADGD 240
           GV+GGMGPHAYTASKHAIVGLTKN ACELG YGIRVNCISPFGVATSMLVNAWR  + GD
Sbjct: 181 GVMGGMGPHAYTASKHAIVGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGD 240

Query: 241 EDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTLRPKDIAEAALYLASDESKYISGHNL 300
            ++D           +V++MEEFVR LANLKG TLR  DIAEAALYLASDESKY++GHNL
Sbjct: 241 VEDD-----------DVEEMEEFVRSLANLKGETLRANDIAEAALYLASDESKYVNGHNL 300

Query: 301 VVDGGITTSRNCVGL 306
           VVDGG+TT+RNCVGL
Sbjct: 301 VVDGGVTTARNCVGL 303

[Close]
BLAST of Spg033543 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 248.1 bits (632), Expect = 9.4e-66
Identity = 132/271 (48.71%), Postives = 179/271 (66.05%), Query Frame = 0 

Query: 28  RLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPCPVSFVHCDV 87
           RL+GK+AI+TGGA GIG   VRLF  HGAKVVI D+++ LG+ LA ++     SF  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 88  SCEEDVENLINSTIRRHGQVDIIFNNAGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGVA 147
           + E DVEN +  T+ +HG++D++F+NAGVL    +   S++D D + F+R M VNV+G A
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVL----EAFGSVLDLDLEAFDRTMAVNVRGAA 124

Query: 148 LGIKHAARVMIPRAT-GCIISTASVAGVLGGMGPHAYTASKHAIVGLTKNTACELGPYGI 207
             IKHAAR M+   T G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG YGI
Sbjct: 125 AFIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGI 184

Query: 208 RVNCISPFGVATSMLVNAWRADGDEDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTLR 267
           RVN ++P+GVAT M  +A+  +                  V  +EE+   L NLKG  L+
Sbjct: 185 RVNGVAPYGVATGM-TSAYNEEA-----------------VKMLEEYGEALGNLKGVVLK 244

Query: 268 PKDIAEAALYLASDESKYISGHNLVVDGGIT 298
            + IAEAAL+LASD+S YISG NLVVDGG +
Sbjct: 245 ARHIAEAALFLASDDSVYISGQNLVVDGGFS 253

[Close]
BLAST of Spg033543 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 1.2e-65
Identity = 128/271 (47.23%), Postives = 178/271 (65.68%), Query Frame = 0 

Query: 27  RRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPCPVSFVHCD 86
           ++LEGKVA++TGGA G+G+AT   F +HGA+VVIAD++   G   A  L      FV CD
Sbjct: 39  KKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETGTKTAKELG-SEAEFVRCD 98

Query: 87  VSCEEDVENLINSTIRRHGQVDIIFNNAGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGV 146
           V+ E D+   +  T+ R+G++D+++NNAG++G    T  SI   D  EFERVMR+NV GV
Sbjct: 99  VTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPM--TPASISQLDMTEFERVMRINVFGV 158

Query: 147 ALGIKHAARVMIPRATGCIISTASVAGVLGGMGPHAYTASKHAIVGLTKNTACELGPYGI 206
             GIKHAA+ MIP  +GCI+ T+SVAGV GG+ PH+YT SK    G+ K+ A EL  +G+
Sbjct: 159 VSGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGV 218

Query: 207 RVNCISPFGVATSMLVNAWRADGDEDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTLR 266
           R+NCISP  VAT + ++             +    P  +E +K+ E V+G+  LKG    
Sbjct: 219 RINCISPGTVATPLTLS------------YLQKVFPKVSE-EKLRETVKGMGELKGAECE 278

Query: 267 PKDIAEAALYLASDESKYISGHNLVVDGGIT 298
             D+A+AALYLAS++ KY++GHNLVVDGG+T
Sbjct: 279 EADVAKAALYLASNDGKYVTGHNLVVDGGMT 293

[Close]
BLAST of Spg033543 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 3.6e-65
Identity = 131/270 (48.52%), Postives = 178/270 (65.93%), Query Frame = 0 

Query: 29  LEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPCPVSFVHCDVS 88
           L+GK+AI+TGGA GIG   VRLF  HGAKVVI D+++ LG+ LA ++     SF  C+V+
Sbjct: 44  LDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNVT 103

Query: 89  CEEDVENLINSTIRRHGQVDIIFNNAGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGVAL 148
            E DVEN +  T+ +HG++D++F+NAGVL    +   S++D D + F+R M VNV+G A 
Sbjct: 104 DETDVENAVKFTVEKHGKLDVLFSNAGVL----EAFGSVLDLDLEAFDRTMAVNVRGAAA 163

Query: 149 GIKHAARVMIPRAT-GCIISTASVAGVLGGMGPHAYTASKHAIVGLTKNTACELGPYGIR 208
            IKHAAR M+   T G I+ T S+A  +GG GPH+YTASKHA++GL ++    LG YGIR
Sbjct: 164 FIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIR 223

Query: 209 VNCISPFGVATSMLVNAWRADGDEDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTLRP 268
           VN ++P+GVAT M  +A+  +                  V  +EE+   L NLKG  L+ 
Sbjct: 224 VNGVAPYGVATGM-TSAYNEEA-----------------VKMLEEYGEALGNLKGVVLKA 283

Query: 269 KDIAEAALYLASDESKYISGHNLVVDGGIT 298
           + IAEAAL+LASD+S YISG NLVVDGG +
Sbjct: 284 RHIAEAALFLASDDSVYISGQNLVVDGGFS 291

[Close]
BLAST of Spg033543 vs. TAIR 10
Match: AT4G03140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 4.0e-64
Identity = 124/272 (45.59%), Postives = 174/272 (63.97%), Query Frame = 0 

Query: 27  RRLEGKVAIVTGGAKGIGEATVRLFAKHGAKVVIADVEDLLGEALADTLSPCPVSFVHCD 86
           R+LEGKVA++TGGA GIG+AT   F  HGAKV+IAD++  +G      L P   ++  CD
Sbjct: 76  RKLEGKVALITGGASGIGKATAGKFISHGAKVIIADIQPQIGRETEQELGP-SCAYFPCD 135

Query: 87  VSCEEDVENLINSTIRRHGQVDIIFNNAGVLGKQSKTHKSIVDFDPDEFERVMRVNVKGV 146
           V+ E D+ N ++  +  H ++DI++NNAG+     KT  SIVD D + F++V+  NV+GV
Sbjct: 136 VTKESDIANAVDFAVSLHTKLDIMYNNAGI---PCKTPPSIVDLDLNVFDKVINTNVRGV 195

Query: 147 ALGIKHAARVMIPRATGCIISTASVAGVLGGMGPHAYTASKHAIVGLTKNTACELGPYGI 206
             GIKHAARVMIPR +G II   SV G++GG+  H Y+ SK A++G+ ++TA EL  + I
Sbjct: 196 MAGIKHAARVMIPRNSGSIICAGSVTGMMGGLAQHTYSVSKSAVIGIVRSTASELCKHRI 255

Query: 207 RVNCISPFGVATSMLVNAWRADGDEDEDECMNFGIPSAAEVDKMEEFVRGLANLKGPTLR 266
           RVNCISPF + TS +++  R              I    +  ++ + V+    L G    
Sbjct: 256 RVNCISPFAITTSFVMDEMRQ-------------IYPGVDDSRLIQIVQSTGVLNGEVCE 315

Query: 267 PKDIAEAALYLASDESKYISGHNLVVDGGITT 299
           P D+A AA+YLASD+SKY++GHNLVVDGG TT
Sbjct: 316 PTDVANAAVYLASDDSKYVNGHNLVVDGGFTT 330

The following BLAST results are available for this feature:
BLAST of Spg033543 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022934318.12.0e-15593.14short-chain dehydrogenase reductase 2a-like [Cucurbita moschata] >KAG6580441.1 S... [more]
XP_023528551.16.0e-15592.81short-chain dehydrogenase reductase 2a-like [Cucurbita pepo subsp. pepo][more]
XP_022983300.18.6e-15492.48short-chain dehydrogenase reductase 2a-like [Cucurbita maxima][more]
XP_022971590.11.6e-15291.83short-chain dehydrogenase reductase 2a-like [Cucurbita maxima][more]
XP_022947494.13.6e-15291.83short-chain dehydrogenase reductase 2a [Cucurbita moschata] >KAG6596319.1 Short-... [more]
BLAST of Spg033543 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SCU03.6e-11067.30Short-chain dehydrogenase reductase 2a OS=Arabidopsis thaliana OX=3702 GN=SDR2a ... [more]
P501603.4e-10566.78Sex determination protein tasselseed-2 OS=Zea mays OX=4577 GN=TS2 PE=2 SV=1[more]
Q94KL76.8e-6947.65Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P... [more]
F1SWA09.8e-6849.09Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1[more]
Q94KL83.2e-6650.37Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P... [more]
BLAST of Spg033543 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1F7B69.9e-15693.14short-chain dehydrogenase reductase 2a-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1J5H24.2e-15492.48short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1I6597.9e-15391.83short-chain dehydrogenase reductase 2a-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1G6Z31.7e-15291.83short-chain dehydrogenase reductase 2a OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A0A0LF571.1e-14987.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G730750 PE=4 SV=1[more]
BLAST of Spg033543 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G51680.12.5e-11167.30NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.29.4e-6648.71NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G26770.11.2e-6547.23NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G29250.13.6e-6548.52NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G03140.14.0e-6445.59NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 260..280
score: 32.49
coord: 204..221
score: 41.2
coord: 105..116
score: 51.1
coord: 157..173
score: 33.98
coord: 183..202
score: 44.24
coord: 33..50
score: 51.21
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 163..171
score: 45.22
coord: 183..202
score: 38.49
coord: 105..116
score: 47.86
NoneNo IPR availableGENE3D3.40.50.720coord: 20..303
e-value: 3.7E-81
score: 274.1
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 38..226
e-value: 1.9E-50
score: 171.6
coord: 245..297
e-value: 1.3E-10
score: 41.3
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 29..214
e-value: 4.0E-9
score: 33.8
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 36..235
e-value: 1.0E-16
score: 58.8
NoneNo IPR availablePANTHERPTHR431803-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)coord: 8..305
NoneNo IPR availablePANTHERPTHR43180:SF41SHORT-CHAIN DEHYDROGENASE REDUCTASE 2Acoord: 8..305
NoneNo IPR availableCDDcd05326secoisolariciresinol-DH_like_SDR_ccoord: 28..297
e-value: 5.44145E-128
score: 362.929
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 30..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg033543.1Spg033543.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004316 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
molecular_function GO:0016491 oxidoreductase activity