Spg033524 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg033524
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	PfkB domain-containing protein
Location	scaffold5: 1510262 .. 1510915 (-)
RNA-Seq Expression	Spg033524
Synteny	Spg033524

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTCTGCAATGGAATCGACGCTCTTCAAGGCTATCCAATTGTCCAAGAAGTTTGGAGGACTCATCTTCTTTGATTTAAATTTGCTGTTGCCTTTGTGGAGACCACGAGACGAAACTCGAGAATACATCAAGAAAGCCTGGAGTGAAGCTGAAACTATTGAGGTTTCAAAGCAAGAATTGAAGCTTCTGTTAGATGAAGAATACTACGAAAAGAAAAGAAACTACAGGCCTCAATACTATGCTCAAACTAATGAACCAACCAAGAATCGGCGAGACTATTACCATGACATGGCGGAGGAAATATCGCCTCAAGCCATTGTTCGTGACGGATGGAACGTTGCCGATTCATTAGTATTCTCCTTCGTTTCATGGCGTGGTGATCGGTACAGAAGATGTGCTCATCACGCCATTCACCTACCATTCACCTGCGACAGGATTGATTCTGGTGATGCTGTTGTGGCTGGGGTCATGAGAAAGCTGACGACTTTTCCTGAGATGTTTGGAAACCAAGACGTTCTGGAACGGCATCTTCGATTCGCAATCACGACCGGGATTATTTCTCAATGGACAATTGGTGCAGTGAGAGGATTTCCTTCTGAAAGTGCAACACATAATCTGAAAGAGCAGGTTTATGTACCAACAATGTGGTAG

mRNA sequence

ATGTCCTCTGCAATGGAATCGACGCTCTTCAAGGCTATCCAATTGTCCAAGAAGTTTGGAGGACTCATCTTCTTTGATTTAAATTTGCTGTTGCCTTTGTGGAGACCACGAGACGAAACTCGAGAATACATCAAGAAAGCCTGGAGTGAAGCTGAAACTATTGAGGTTTCAAAGCAAGAATTGAAGCTTCTGTTAGATGAAGAATACTACGAAAAGAAAAGAAACTACAGGCCTCAATACTATGCTCAAACTAATGAACCAACCAAGAATCGGCGAGACTATTACCATGACATGGCGGAGGAAATATCGCCTCAAGCCATTGTTCGTGACGGATGGAACGTTGCCGATTCATTAGTATTCTCCTTCGTTTCATGGCGTGGTGATCGGTACAGAAGATGTGCTCATCACGCCATTCACCTACCATTCACCTGCGACAGGATTGATTCTGGTGATGCTGTTGTGGCTGGGGTCATGAGAAAGCTGACGACTTTTCCTGAGATGTTTGGAAACCAAGACGTTCTGGAACGGCATCTTCGATTCGCAATCACGACCGGGATTATTTCTCAATGGACAATTGGTGCAGTGAGAGGATTTCCTTCTGAAAGTGCAACACATAATCTGAAAGAGCAGGTTTATGTACCAACAATGTGGTAG

Coding sequence (CDS)

ATGTCCTCTGCAATGGAATCGACGCTCTTCAAGGCTATCCAATTGTCCAAGAAGTTTGGAGGACTCATCTTCTTTGATTTAAATTTGCTGTTGCCTTTGTGGAGACCACGAGACGAAACTCGAGAATACATCAAGAAAGCCTGGAGTGAAGCTGAAACTATTGAGGTTTCAAAGCAAGAATTGAAGCTTCTGTTAGATGAAGAATACTACGAAAAGAAAAGAAACTACAGGCCTCAATACTATGCTCAAACTAATGAACCAACCAAGAATCGGCGAGACTATTACCATGACATGGCGGAGGAAATATCGCCTCAAGCCATTGTTCGTGACGGATGGAACGTTGCCGATTCATTAGTATTCTCCTTCGTTTCATGGCGTGGTGATCGGTACAGAAGATGTGCTCATCACGCCATTCACCTACCATTCACCTGCGACAGGATTGATTCTGGTGATGCTGTTGTGGCTGGGGTCATGAGAAAGCTGACGACTTTTCCTGAGATGTTTGGAAACCAAGACGTTCTGGAACGGCATCTTCGATTCGCAATCACGACCGGGATTATTTCTCAATGGACAATTGGTGCAGTGAGAGGATTTCCTTCTGAAAGTGCAACACATAATCTGAAAGAGCAGGTTTATGTACCAACAATGTGGTAG

Protein sequence

MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQELKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVFSFVSWRGDRYRRCAHHAIHLPFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQDVLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW

Homology

[Close]

BLAST of Spg033524 vs. NCBI nr
Match: XP_022145754.1 (fructokinase-like 1, chloroplastic [Momordica charantia])

HSP 1 Score: 310.1 bits (793), Expect = 1.5e-80
Identity = 162/225 (72.00%), Postives = 175/225 (77.78%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           MS+AMESTLFKAIQLSKKFGGLIFFDLNLLLPLWR RDETREYIKKAW+EA+ IEVS+QE
Sbjct: 299 MSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQE 358

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRDYYH   EEISP       W+    L+F
Sbjct: 359 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPL------WHDRLRLLF 418

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDAVVAG+MRKLTTFPEMF NQD
Sbjct: 419 VTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQD 478

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
            LER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 479 XLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 517

[Close]

BLAST of Spg033524 vs. NCBI nr
Match: XP_031737955.1 (fructokinase-like 1, chloroplastic [Cucumis sativus] >KGN58805.1 hypothetical protein Csa_000812 [Cucumis sativus])

HSP 1 Score: 298.5 bits (763), Expect = 4.6e-77
Identity = 155/225 (68.89%), Postives = 172/225 (76.44%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +S+A+E TLFKAIQLSKKFGGLIFFDLNL LPLW+ RDETREYIKKAW EA+ IEVS+QE
Sbjct: 293 LSTAIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQE 352

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRD+YH   EE+SP       W+    L+F
Sbjct: 353 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPL------WHDRLKLLF 412

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDAVVAG+MRKLTTFPEM  NQD
Sbjct: 413 VTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQD 472

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
           VLER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 473 VLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

[Close]

BLAST of Spg033524 vs. NCBI nr
Match: XP_038906230.1 (fructokinase-like 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 298.1 bits (762), Expect = 6.1e-77
Identity = 155/225 (68.89%), Postives = 173/225 (76.89%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +S+AM+STLFKAIQLSKKFGGLIFFDLNL LPLW+ RDETREYIKKAW+EA+ IEVS+QE
Sbjct: 292 LSTAMKSTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWNEADIIEVSRQE 351

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRD+YH   EEISP       W+    L+F
Sbjct: 352 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPL------WHDRLKLLF 411

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDAVVAG+MRKLTTFPE+  NQD
Sbjct: 412 VTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEILVNQD 471

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
            LER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 472 ALERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 510

[Close]

BLAST of Spg033524 vs. NCBI nr
Match: XP_016899692.1 (PREDICTED: fructokinase-like 1, chloroplastic [Cucumis melo] >KAA0044141.1 fructokinase-like 1 [Cucumis melo var. makuwa] >TYK24996.1 fructokinase-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 296.2 bits (757), Expect = 2.3e-76
Identity = 154/225 (68.44%), Postives = 172/225 (76.44%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +S+ +E TLFKAIQLSKKFGGLIFFDLNL LPLW+ R+ETREYIKKAW+EA+ IEVS+QE
Sbjct: 293 LSTVIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQE 352

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRD+YH   EEISP       W+    L+F
Sbjct: 353 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPL------WHDHLKLLF 412

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDAVVAG+MRKLTTFPEM  NQD
Sbjct: 413 VTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQD 472

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
           VLER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 473 VLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

[Close]

BLAST of Spg033524 vs. NCBI nr
Match: KAG6596368.1 (Fructokinase-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 295.4 bits (755), Expect = 3.9e-76
Identity = 153/225 (68.00%), Postives = 173/225 (76.89%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +S+ ME+TLFKAIQLSKKFGGLIFFDLNL LPLWR RDETR+YI+KAW++A+ IEVS+QE
Sbjct: 179 LSTTMEATLFKAIQLSKKFGGLIFFDLNLPLPLWRSRDETRKYIEKAWNQADIIEVSRQE 238

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRD+YH   EEISP       W+    L+ 
Sbjct: 239 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPL------WHDHLKLLL 298

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDA+VAG+MRKLTTFPEMF NQD
Sbjct: 299 VTDGTLRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFENQD 358

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
           VLER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 359 VLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 397

[Close]

BLAST of Spg033524 vs. ExPASy Swiss-Prot
Match: Q9M394 (Fructokinase-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN1 PE=1 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 2.6e-70
Identity = 139/223 (62.33%), Postives = 163/223 (73.09%), Query Frame = 0


Query: 2   SSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQEL 61
           S  M+STLF AIQ SKKFGGLIFFDLNL LPLWR R+ETR+ IKKAW+EA  IEVS+QEL
Sbjct: 254 SPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSRNETRKLIKKAWNEANIIEVSQQEL 313

Query: 62  KLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEIS-------PQAIVRDGWNV 121
           + LLDE+YYE++RNY PQY+A+  + TKNRRDYYH   EEI           +V DG   
Sbjct: 314 EFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHYTPEEIKSLWHDKLKLLVVTDG--- 373

Query: 122 ADSLVFSFVSWRGDRYRRCAHHAIHLPFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQDVL 181
             +L   + +   D         +  PFTCDR  SGDAVVAG+MRKLTT PEMF +QDV+
Sbjct: 374 --TLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTCPEMFEDQDVM 433

Query: 182 ERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
           ER LRFA+  GII+QWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 434 ERQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPSMW 471

[Close]

BLAST of Spg033524 vs. ExPASy Swiss-Prot
Match: F4I0K2 (Fructokinase-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN2 PE=1 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 3.6e-24
Identity = 70/207 (33.82%), Postives = 106/207 (51.21%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +   M ST  +AI++SK+ G +IF+DLNL LPLW   +ET+ +I++ W+ A+ IE++KQE
Sbjct: 355 LDKKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWNLADVIEITKQE 414

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ L   E  E       ++  + N+ +K    + H       P   V   W+    ++F
Sbjct: 415 LEFLCGIEPTE-------EFDTENNDISK----FVH------YPPETVEQLWHENLKVLF 474

Query: 121 SFVSWRGDRYRRCAHHAI--------HLPFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y    H+            PFT D   SGD +VAG++R LT  P++  N+ 
Sbjct: 475 VTNGTSKIHYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLMNNKG 534

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFP 200
            LER  R+AI  GII QW +   RG+P
Sbjct: 535 YLERTARYAIECGIIDQWLLAQTRGYP 544

[Close]

BLAST of Spg033524 vs. ExPASy Swiss-Prot
Match: Q9M1B9 (Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 8.7e-10
Identity = 50/204 (24.51%), Postives = 87/204 (42.65%), Query Frame = 0


Query: 7   STLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQELKLLLD 66
           S   KA++++K+ G L+ +D NL LPLW  ++E ++ I   W +AE I+VS +EL  L  
Sbjct: 152 SAHLKAMEVAKEAGALLSYDPNLRLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLTG 211

Query: 67  EEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVFSFVSWR 126
                                         D  ++ +  ++    W+    L+   +  +
Sbjct: 212 S-----------------------------DKVDDETALSL----WHSNLKLLLVTLGEK 271

Query: 127 GDRYRRCAHHAIHLPFTCDRID---SGDAVVAGVMRKLTTFPEMFGNQDVLERHLRFAIT 186
           G RY   +      PF  D +D   +GD+ V  ++ K+     +  ++  L   LR A  
Sbjct: 272 GCRYYTKSFRGSVDPFHVDAVDTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRLANA 322

Query: 187 TGIISQWTIGAVRGFPSESATHNL 208
            G I+    GA+   P+ES   +L
Sbjct: 332 CGAITTTKKGAIPALPTESEVQSL 322

[Close]

BLAST of Spg033524 vs. ExPASy Swiss-Prot
Match: Q6XZ79 (Fructokinase-1 OS=Zea mays OX=4577 GN=FRK1 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.3e-08
Identity = 45/203 (22.17%), Postives = 83/203 (40.89%), Query Frame = 0


Query: 11  KAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQELKLLLDEEYY 70
           +A++++K+ G L+ +D NL   LW  R+E R  I   W +A+ ++VS+ EL+ L   +  
Sbjct: 153 RAMEIAKEAGALLSYDPNLREALWPSREEARTQILSIWDQADIVKVSEVELEFLTGIDSV 212

Query: 71  EKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVFSFVSWRGDRY 130
           E                                   +V   W     L+   +  +G +Y
Sbjct: 213 E---------------------------------DDVVMKLWRPTMKLLLVTLGDQGCKY 272

Query: 131 RRCAHHAIHLPFTCDRID---SGDAVVAGVMRKLTTFPEMFGNQDVLERHLRFAITTGII 190
                H     F   ++D   +GDA V  +++++   P    ++  L   ++FA   G I
Sbjct: 273 YARDFHGAVPSFKVQQVDTTGAGDAFVGALLQRIVKDPSSLQDEKKLVESIKFANACGAI 322

Query: 191 SQWTIGAVRGFPSESATHNLKEQ 211
           +    GA+   P+E+    L E+
Sbjct: 333 TTTKKGAIPSLPTEAEVLQLIEK 322

[Close]

BLAST of Spg033524 vs. ExPASy Swiss-Prot
Match: Q9SID0 (Probable fructokinase-1 OS=Arabidopsis thaliana OX=3702 GN=At2g31390 PE=2 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.8e-08
Identity = 48/207 (23.19%), Postives = 85/207 (41.06%), Query Frame = 0


Query: 7   STLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQELKLLLD 66
           S   KA++++K+ G L+ +D NL  PLW  ++E +  I   W +AE I+VS  EL+ L  
Sbjct: 151 SAHLKAMEVAKEAGALLSYDPNLREPLWPSKEEAKTQIMSIWDKAEIIKVSDVELEFLTG 210

Query: 67  EEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVFSFVSWR 126
                                  N+ D                  W+    L+   +  +
Sbjct: 211 ----------------------SNKID-----------DETALTLWHPNLKLLLVTLGEK 270

Query: 127 GDRYRRCAHHAIHLPFTCDRID---SGDAVVAGVMRKLTTFPEMFGNQDVLERHLRFAIT 186
           G RY          PF  + +D   +GD+ V  ++ ++     +  +++ L + LRFA  
Sbjct: 271 GCRYYTKTFKGAVDPFHVNAVDTTGAGDSFVGALLNQIVDDRSVLEDEERLRKVLRFANA 324

Query: 187 TGIISQWTIGAVRGFPSESATHNLKEQ 211
            G I+    GA+   PS++   +  E+
Sbjct: 331 CGAITTTKKGAIPALPSDAEVRSFLEK 324

[Close]

BLAST of Spg033524 vs. ExPASy TrEMBL
Match: A0A6J1CW73 (fructokinase-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015134 PE=4 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 7.5e-81
Identity = 162/225 (72.00%), Postives = 175/225 (77.78%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           MS+AMESTLFKAIQLSKKFGGLIFFDLNLLLPLWR RDETREYIKKAW+EA+ IEVS+QE
Sbjct: 299 MSTAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRSRDETREYIKKAWNEADIIEVSRQE 358

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRDYYH   EEISP       W+    L+F
Sbjct: 359 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDYYHYTPEEISPL------WHDRLRLLF 418

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDAVVAG+MRKLTTFPEMF NQD
Sbjct: 419 VTDGTMRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMFENQD 478

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
            LER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 479 XLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 517

[Close]

BLAST of Spg033524 vs. ExPASy TrEMBL
Match: A0A0A0LA03 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G732620 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 2.2e-77
Identity = 155/225 (68.89%), Postives = 172/225 (76.44%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +S+A+E TLFKAIQLSKKFGGLIFFDLNL LPLW+ RDETREYIKKAW EA+ IEVS+QE
Sbjct: 293 LSTAIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSRDETREYIKKAWKEADIIEVSRQE 352

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRD+YH   EE+SP       W+    L+F
Sbjct: 353 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEELSPL------WHDRLKLLF 412

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDAVVAG+MRKLTTFPEM  NQD
Sbjct: 413 VTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQD 472

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
           VLER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 473 VLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

[Close]

BLAST of Spg033524 vs. ExPASy TrEMBL
Match: A0A5A7TRC7 (Fructokinase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001140 PE=4 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 1.1e-76
Identity = 154/225 (68.44%), Postives = 172/225 (76.44%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +S+ +E TLFKAIQLSKKFGGLIFFDLNL LPLW+ R+ETREYIKKAW+EA+ IEVS+QE
Sbjct: 293 LSTVIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQE 352

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRD+YH   EEISP       W+    L+F
Sbjct: 353 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPL------WHDHLKLLF 412

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDAVVAG+MRKLTTFPEM  NQD
Sbjct: 413 VTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQD 472

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
           VLER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 473 VLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

[Close]

BLAST of Spg033524 vs. ExPASy TrEMBL
Match: A0A1S4DUM6 (fructokinase-like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486334 PE=4 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 1.1e-76
Identity = 154/225 (68.44%), Postives = 172/225 (76.44%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +S+ +E TLFKAIQLSKKFGGLIFFDLNL LPLW+ R+ETREYIKKAW+EA+ IEVS+QE
Sbjct: 293 LSTVIEPTLFKAIQLSKKFGGLIFFDLNLPLPLWKSREETREYIKKAWNEADIIEVSRQE 352

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRD+YH   EEISP       W+    L+F
Sbjct: 353 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYTPEEISPL------WHDHLKLLF 412

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDAVVAG+MRKLTTFPEM  NQD
Sbjct: 413 VTDGTLRIHYYSPSFHGVAIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTFPEMLENQD 472

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
           VLER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 473 VLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 511

[Close]

BLAST of Spg033524 vs. ExPASy TrEMBL
Match: A0A6J1FBN1 (fructokinase-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442595 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.6e-75
Identity = 151/225 (67.11%), Postives = 173/225 (76.89%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +S+ ME+TLFKAIQLSKKFGGLIFFDLNL LPLWR RD+TR+YI+KAW++A+ IEVS+QE
Sbjct: 284 LSTTMEATLFKAIQLSKKFGGLIFFDLNLSLPLWRSRDKTRKYIEKAWNQADIIEVSRQE 343

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ LLDEEYYEKKRNYRPQYYAQT E TKNRRD+YH   EEISP       W+    L+ 
Sbjct: 344 LEFLLDEEYYEKKRNYRPQYYAQTIEQTKNRRDHYHYSPEEISPL------WHDHLKLLL 403

Query: 121 SFVSWRGDRYRRCAHHAIHL--------PFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y   + H + +        PFTCDR  SGDA+VAG+MRKLTTFPEMF +QD
Sbjct: 404 VTDGTLRIHYYSPSFHGVVIGTEDVLITPFTCDRTGSGDALVAGIMRKLTTFPEMFESQD 463

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
           VLER LRFAI  GIISQWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 464 VLERQLRFAIAAGIISQWTIGAVRGFPTESATQNLKEQVYVPSMW 502

[Close]

BLAST of Spg033524 vs. TAIR 10
Match: AT3G54090.1 (fructokinase-like 1 )

HSP 1 Score: 266.5 bits (680), Expect = 1.8e-71
Identity = 139/223 (62.33%), Postives = 163/223 (73.09%), Query Frame = 0


Query: 2   SSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQEL 61
           S  M+STLF AIQ SKKFGGLIFFDLNL LPLWR R+ETR+ IKKAW+EA  IEVS+QEL
Sbjct: 254 SPTMQSTLFTAIQWSKKFGGLIFFDLNLPLPLWRSRNETRKLIKKAWNEANIIEVSQQEL 313

Query: 62  KLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEIS-------PQAIVRDGWNV 121
           + LLDE+YYE++RNY PQY+A+  + TKNRRDYYH   EEI           +V DG   
Sbjct: 314 EFLLDEDYYERRRNYTPQYFAEDFDQTKNRRDYYHYTPEEIKSLWHDKLKLLVVTDG--- 373

Query: 122 ADSLVFSFVSWRGDRYRRCAHHAIHLPFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQDVL 181
             +L   + +   D         +  PFTCDR  SGDAVVAG+MRKLTT PEMF +QDV+
Sbjct: 374 --TLRLHYYTPTFDGVVIGTEDVLITPFTCDRTGSGDAVVAGIMRKLTTCPEMFEDQDVM 433

Query: 182 ERHLRFAITTGIISQWTIGAVRGFPSESATHNLKEQVYVPTMW 218
           ER LRFA+  GII+QWTIGAVRGFP+ESAT NLKEQVYVP+MW
Sbjct: 434 ERQLRFAVAAGIIAQWTIGAVRGFPTESATQNLKEQVYVPSMW 471

[Close]

BLAST of Spg033524 vs. TAIR 10
Match: AT1G69200.1 (fructokinase-like 2 )

HSP 1 Score: 113.2 bits (282), Expect = 2.6e-25
Identity = 70/207 (33.82%), Postives = 106/207 (51.21%), Query Frame = 0


Query: 1   MSSAMESTLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQE 60
           +   M ST  +AI++SK+ G +IF+DLNL LPLW   +ET+ +I++ W+ A+ IE++KQE
Sbjct: 357 LDKKMMSTTIQAIKISKQLGNVIFYDLNLPLPLWHSSEETKSFIQEVWNLADVIEITKQE 416

Query: 61  LKLLLDEEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVF 120
           L+ L   E  E       ++  + N+ +K    + H       P   V   W+    ++F
Sbjct: 417 LEFLCGIEPTE-------EFDTENNDISK----FVH------YPPETVEQLWHENLKVLF 476

Query: 121 SFVSWRGDRYRRCAHHAI--------HLPFTCDRIDSGDAVVAGVMRKLTTFPEMFGNQD 180
                    Y    H+            PFT D   SGD +VAG++R LT  P++  N+ 
Sbjct: 477 VTNGTSKIHYYTKEHNGAVSGMEDVPITPFTRDMSASGDGIVAGLIRMLTVQPDLMNNKG 536

Query: 181 VLERHLRFAITTGIISQWTIGAVRGFP 200
            LER  R+AI  GII QW +   RG+P
Sbjct: 537 YLERTARYAIECGIIDQWLLAQTRGYP 546

[Close]

BLAST of Spg033524 vs. TAIR 10
Match: AT3G59480.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 65.5 bits (158), Expect = 6.2e-11
Identity = 50/204 (24.51%), Postives = 87/204 (42.65%), Query Frame = 0


Query: 7   STLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQELKLLLD 66
           S   KA++++K+ G L+ +D NL LPLW  ++E ++ I   W +AE I+VS +EL  L  
Sbjct: 152 SAHLKAMEVAKEAGALLSYDPNLRLPLWPSKEEAQKQILSIWDKAEVIKVSDEELMFLTG 211

Query: 67  EEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVFSFVSWR 126
                                         D  ++ +  ++    W+    L+   +  +
Sbjct: 212 S-----------------------------DKVDDETALSL----WHSNLKLLLVTLGEK 271

Query: 127 GDRYRRCAHHAIHLPFTCDRID---SGDAVVAGVMRKLTTFPEMFGNQDVLERHLRFAIT 186
           G RY   +      PF  D +D   +GD+ V  ++ K+     +  ++  L   LR A  
Sbjct: 272 GCRYYTKSFRGSVDPFHVDAVDTTGAGDSFVGALLCKIVDDRAVLEDEARLREVLRLANA 322

Query: 187 TGIISQWTIGAVRGFPSESATHNL 208
            G I+    GA+   P+ES   +L
Sbjct: 332 CGAITTTKKGAIPALPTESEVQSL 322

[Close]

BLAST of Spg033524 vs. TAIR 10
Match: AT2G31390.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 60.5 bits (145), Expect = 2.0e-09
Identity = 48/207 (23.19%), Postives = 85/207 (41.06%), Query Frame = 0


Query: 7   STLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQELKLLLD 66
           S   KA++++K+ G L+ +D NL  PLW  ++E +  I   W +AE I+VS  EL+ L  
Sbjct: 151 SAHLKAMEVAKEAGALLSYDPNLREPLWPSKEEAKTQIMSIWDKAEIIKVSDVELEFLTG 210

Query: 67  EEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVFSFVSWR 126
                                  N+ D                  W+    L+   +  +
Sbjct: 211 ----------------------SNKID-----------DETALTLWHPNLKLLLVTLGEK 270

Query: 127 GDRYRRCAHHAIHLPFTCDRID---SGDAVVAGVMRKLTTFPEMFGNQDVLERHLRFAIT 186
           G RY          PF  + +D   +GD+ V  ++ ++     +  +++ L + LRFA  
Sbjct: 271 GCRYYTKTFKGAVDPFHVNAVDTTGAGDSFVGALLNQIVDDRSVLEDEERLRKVLRFANA 324

Query: 187 TGIISQWTIGAVRGFPSESATHNLKEQ 211
            G I+    GA+   PS++   +  E+
Sbjct: 331 CGAITTTKKGAIPALPSDAEVRSFLEK 324

[Close]

BLAST of Spg033524 vs. TAIR 10
Match: AT1G06030.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 58.2 bits (139), Expect = 9.8e-09
Identity = 50/209 (23.92%), Postives = 84/209 (40.19%), Query Frame = 0


Query: 7   STLFKAIQLSKKFGGLIFFDLNLLLPLWRPRDETREYIKKAWSEAETIEVSKQELKLLLD 66
           S   KA++++K+ G L+ +D NL  PLW   +E R+ I   W +A+ I+VS  EL+ L  
Sbjct: 153 SAHMKAMEVAKEAGALLSYDPNLREPLWPSPEEARKQIMSIWDKADIIKVSDVELEFLTG 212

Query: 67  EEYYEKKRNYRPQYYAQTNEPTKNRRDYYHDMAEEISPQAIVRDGWNVADSLVFSFVSWR 126
            +  + +                                      W+    L+   +   
Sbjct: 213 NKTIDDE---------------------------------TAMSLWHPNLKLLLVTLGEN 272

Query: 127 GDRYRRCAHHAIHLPFTCDRID---SGDAVVAGVMRKLTTFPEMFGNQDVLERHLRFAIT 186
           G RY     H     F  D +D   +GD+ V  ++ ++     +   ++ L + LRFA  
Sbjct: 273 GCRYYTKDFHGSVETFHVDAVDTTGAGDSFVGALLNQIVDDQSVLEEEERLRKVLRFANA 328

Query: 187 TGIISQWTIGAVRGFPSE-SATHNLKEQV 212
            G I+    GA+   P++  A   LK QV
Sbjct: 333 CGAITTTKKGAIPALPTDCEALSFLKIQV 328

The following BLAST results are available for this feature:

BLAST of Spg033524 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145754.1	1.5e-80	72.00	fructokinase-like 1, chloroplastic [Momordica charantia]	[more]
XP_031737955.1	4.6e-77	68.89	fructokinase-like 1, chloroplastic [Cucumis sativus] >KGN58805.1 hypothetical pr...	[more]
XP_038906230.1	6.1e-77	68.89	fructokinase-like 1, chloroplastic [Benincasa hispida]	[more]
XP_016899692.1	2.3e-76	68.44	PREDICTED: fructokinase-like 1, chloroplastic [Cucumis melo] >KAA0044141.1 fruct...	[more]
KAG6596368.1	3.9e-76	68.00	Fructokinase-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of Spg033524 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M394	2.6e-70	62.33	Fructokinase-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN1 PE=1 ...	[more]
F4I0K2	3.6e-24	33.82	Fructokinase-like 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FLN2 PE=1 ...	[more]
Q9M1B9	8.7e-10	24.51	Probable fructokinase-4 OS=Arabidopsis thaliana OX=3702 GN=At3g59480 PE=2 SV=1	[more]
Q6XZ79	1.3e-08	22.17	Fructokinase-1 OS=Zea mays OX=4577 GN=FRK1 PE=1 SV=1	[more]
Q9SID0	2.8e-08	23.19	Probable fructokinase-1 OS=Arabidopsis thaliana OX=3702 GN=At2g31390 PE=2 SV=1	[more]

BLAST of Spg033524 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CW73	7.5e-81	72.00	fructokinase-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101513...	[more]
A0A0A0LA03	2.2e-77	68.89	PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G732620 PE=4 S...	[more]
A0A5A7TRC7	1.1e-76	68.44	Fructokinase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G...	[more]
A0A1S4DUM6	1.1e-76	68.44	fructokinase-like 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103486334 PE=4 ...	[more]
A0A6J1FBN1	1.6e-75	67.11	fructokinase-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111442595...	[more]

BLAST of Spg033524 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G54090.1	1.8e-71	62.33	fructokinase-like 1	[more]
AT1G69200.1	2.6e-25	33.82	fructokinase-like 2	[more]
AT3G59480.1	6.2e-11	24.51	pfkB-like carbohydrate kinase family protein	[more]
AT2G31390.1	2.0e-09	23.19	pfkB-like carbohydrate kinase family protein	[more]
AT1G06030.1	9.8e-09	23.92	pfkB-like carbohydrate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029056	Ribokinase-like	GENE3D	3.40.1190.20		coord: 1..212 e-value: 3.6E-12 score: 48.2
IPR029056	Ribokinase-like	SUPERFAMILY	53613	Ribokinase-like	coord: 4..201
None	No IPR available	PANTHER	PTHR43085:SF10	FRUCTOKINASE-LIKE 1, CHLOROPLASTIC	coord: 2..215
None	No IPR available	PANTHER	PTHR43085	HEXOKINASE FAMILY MEMBER	coord: 2..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg033524.1	Spg033524.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0071704	organic substance metabolic process
molecular_function	GO:0016772	transferase activity, transferring phosphorus-containing groups