Spg033516 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg033516 vs. NCBI nr
Match: XP_038905817.1 (heavy metal-associated isoprenylated plant protein 5-like [Benincasa hispida])

HSP 1 Score: 562.8 bits (1449), Expect = 2.3e-156
Identity = 326/390 (83.59%), Postives = 343/390 (87.95%), Query Frame = 0

Query: 1   MGEQNV-EAPPKAEGEKKPAAAPAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHCEGCA 60
           M EQ V E+P K E EKKP A PAP PAAVP   DGGAKKE GGAITAIYKIDMHCEGCA
Sbjct: 1   MVEQKVEESPAKNEAEKKP-ATPAPAPAAVPVATDGGAKKEDGGAITAIYKIDMHCEGCA 60

Query: 61  KKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQPKKDGG 120
           KKIKRAVRHVKDVESV ADC AN+LTVIGKMDV+ VK+KLELKTKKKVELISPQPKKD  
Sbjct: 61  KKIKRAVRHVKDVESVKADCGANQLTVIGKMDVVAVKQKLELKTKKKVELISPQPKKDAA 120

Query: 121 AAP-AGEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKINGVQSVSF 180
           AAP A EKKA+EKKPEEKKAPEEKPKESTVVLKIRLHC+GCIQKIRRIISKINGVQSV  
Sbjct: 121 AAPAAAEKKADEKKPEEKKAPEEKPKESTVVLKIRLHCDGCIQKIRRIISKINGVQSVDL 180

Query: 181 DGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEE-PA-AAAAPAAV 240
           DGAKDLVT+KG MDVKQLEPYLKDKLKR+VEI+PPKKEEPA EKKKEE PA AAAAP   
Sbjct: 181 DGAKDLVTVKGTMDVKQLEPYLKDKLKRSVEIIPPKKEEPAGEKKKEESPAPAAAAPGVG 240

Query: 241 EKKKEE--GGGEKKEKEGGGGEKKEKEGGGGEKKEKEGGGGEKKEGESKPAAPAAAEGGG 300
           EKKKEE  GGGEKKEKEGGGGEKKEKEGGGG      GGGGEKK+GESKPA   AAEGGG
Sbjct: 241 EKKKEEAGGGGEKKEKEGGGGEKKEKEGGGG------GGGGEKKDGESKPAPLPAAEGGG 300

Query: 301 AKAVEVINKMDYYGGYPYQQLYYEAPMQYHSYSMEANPSYYNPNYGYVNHGYTDHRYDVV 360
           AK  +V+NK++YYGGYPY+ LYYEAPMQYHSYSMEANPSYYNPNYGY N GYTDHRY VV
Sbjct: 301 AKPADVVNKLEYYGGYPYEPLYYEAPMQYHSYSMEANPSYYNPNYGYNNLGYTDHRYGVV 360

Query: 361 PMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           PMD+H+PHHM QHDQPQMFSDENPNACSVM
Sbjct: 361 PMDHHYPHHM-QHDQPQMFSDENPNACSVM 382

BLAST of Spg033516 vs. NCBI nr
Match: XP_008442706.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo] >KAA0043995.1 heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo var. makuwa] >TYK25145.1 heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 553.9 bits (1426), Expect = 1.1e-153
Identity = 330/395 (83.54%), Postives = 337/395 (85.32%), Query Frame = 0

Query: 1   MGEQNVEAP-PKAEGEKKPAA-APAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHCEGC 60
           MGE  VE P  K E EKKPAA  PAP PAAVP   DGGAKKE GGAITAIYKIDMHCEGC
Sbjct: 1   MGEAKVEEPAAKNESEKKPAAPVPAPAPAAVPVATDGGAKKEDGGAITAIYKIDMHCEGC 60

Query: 61  AKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQPKKDG 120
           AKKIKRAVRHVKDVESV ADC ANKLTVIGKMDV+ VK+KLELKTKKKVELISPQPKKD 
Sbjct: 61  AKKIKRAVRHVKDVESVKADCGANKLTVIGKMDVVAVKQKLELKTKKKVELISPQPKKDA 120

Query: 121 GAA-----PAGEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKINGV 180
            AA      A EKKA+EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRII KINGV
Sbjct: 121 PAAAAAAPAAAEKKADEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIILKINGV 180

Query: 181 QSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAP 240
            SV  DG KDLVT+KG MDVKQLEPYLKDKLKRNVEIVPPKKEEPA EKKKEE  A AAP
Sbjct: 181 HSVDLDGVKDLVTVKGTMDVKQLEPYLKDKLKRNVEIVPPKKEEPAGEKKKEESPAPAAP 240

Query: 241 A-AVEKKKEE--GGGEKKEKEGGGGEKKEKEGGGGEKKEKEGGGGEKKEGESKPAAPAAA 300
           A   EKKKEE  GGGEKKEKEGGGGEK  KE GGGEKKEKE GGGEKKEGESKPA P AA
Sbjct: 241 AGGGEKKKEEAGGGGEKKEKEGGGGEK--KEAGGGEKKEKE-GGGEKKEGESKPAPPPAA 300

Query: 301 EGGGAKAVEVINKMDYYGGYPYQQLYYEAPMQYHSYSMEANPS-YYNPNYGYVNHGYTDH 360
           EGGGAK  EV+NK +YYGGYPYQ LYYEAPMQY SYSMEANPS YYN NYGY NHGY DH
Sbjct: 301 EGGGAKPAEVVNKFEYYGGYPYQPLYYEAPMQYQSYSMEANPSYYYNHNYGYNNHGYVDH 360

Query: 361 RYDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           RYDVVPMD HFPHHM Q+DQPQMFSDENPNACSVM
Sbjct: 361 RYDVVPMDPHFPHHM-QNDQPQMFSDENPNACSVM 391

BLAST of Spg033516 vs. NCBI nr
Match: XP_023527959.1 (heavy metal-associated isoprenylated plant protein 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545.4 bits (1404), Expect = 3.9e-151
Identity = 323/394 (81.98%), Postives = 339/394 (86.04%), Query Frame = 0

Query: 1   MGEQNVE---APPKAEGEKK--PAAAPAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHC 60
           MGEQ VE   A  K E EKK   A APAP PAAVPA A+GG KKE GGAITA+YKIDMHC
Sbjct: 1   MGEQKVEEAVAAAKNEVEKKSGAAVAPAPAPAAVPAVAEGGVKKEDGGAITAVYKIDMHC 60

Query: 61  EGCAKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQPK 120
           EGCAKKIKRAVRHVKDVESV  DC ANKLTVIGKMDV+ VKEKLELKTKKKVELISPQPK
Sbjct: 61  EGCAKKIKRAVRHVKDVESVKTDCAANKLTVIGKMDVVAVKEKLELKTKKKVELISPQPK 120

Query: 121 KDGGAAPA-GEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKINGVQ 180
           KDGGA  A  EKKA+EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIR+II KINGVQ
Sbjct: 121 KDGGAVAAPAEKKADEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRKIILKINGVQ 180

Query: 181 SVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAPA 240
           SV+ DGAKDLVT+KG MDVKQLEPYLKDKLKRNVEI+PPKKEEPA EKKKEE  A AA  
Sbjct: 181 SVALDGAKDLVTVKGTMDVKQLEPYLKDKLKRNVEIIPPKKEEPAGEKKKEESPAPAAEV 240

Query: 241 AVEKKKEEGGGEKKEKE-GGGGEKKEKEGGGGEKKEKEGGGGEKKEG-ESKPA-APAAAE 300
           AV    E GGGEKK++E GGGGEKKEKEGGG    EK+ GGGEKKE  ESKPA  PAAAE
Sbjct: 241 AVPVAVEAGGGEKKKEEAGGGGEKKEKEGGG----EKKEGGGEKKEAVESKPAPPPAAAE 300

Query: 301 GGGAKAVEVINKMDYYGGYPYQQLYYEAPMQY-HSYSMEANPSYYNPNYGYVNHGYTDHR 360
           GGGA  V+V+NK++YYGGYPYQ LYYEAPMQY HSYSMEANPSYY PNYGY NHGYTDHR
Sbjct: 301 GGGAMPVDVMNKLEYYGGYPYQPLYYEAPMQYHHSYSMEANPSYYTPNYGYANHGYTDHR 360

Query: 361 YDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           YDVVPMD+HFPHHMQQ DQPQMFSDENPNACSVM
Sbjct: 361 YDVVPMDHHFPHHMQQ-DQPQMFSDENPNACSVM 389

BLAST of Spg033516 vs. NCBI nr
Match: XP_022934938.1 (heavy metal-associated isoprenylated plant protein 5-like [Cucurbita moschata])

HSP 1 Score: 545.0 bits (1403), Expect = 5.0e-151
Identity = 323/394 (81.98%), Postives = 340/394 (86.29%), Query Frame = 0

Query: 1   MGEQNVE---APPKAEGEKKPAA--APAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHC 60
           MGEQ VE   A  K E EKK AA  APAP PAAVPA A+GGAKKE GGAITA+YKIDMHC
Sbjct: 1   MGEQKVEEAVAAAKNEVEKKSAAAVAPAPAPAAVPAVAEGGAKKEDGGAITAVYKIDMHC 60

Query: 61  EGCAKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQPK 120
           EGCAKKIKR VRHVKDVESV  DC ANKLTVIGKMDV+ VKEKLELKTKKKVELISPQPK
Sbjct: 61  EGCAKKIKRTVRHVKDVESVKTDCGANKLTVIGKMDVVAVKEKLELKTKKKVELISPQPK 120

Query: 121 KDGGAAPA-GEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKINGVQ 180
           KDGGA  A  EKKA+EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIR+II KINGVQ
Sbjct: 121 KDGGAVAAPAEKKADEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRKIILKINGVQ 180

Query: 181 SVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAPA 240
           SV+ DGAKDLVT+KG MDVKQLEPYLKDKLKRNVE++PPKKEEPA EKKKEE  A AA  
Sbjct: 181 SVALDGAKDLVTVKGTMDVKQLEPYLKDKLKRNVEVIPPKKEEPAGEKKKEESPAPAAVV 240

Query: 241 AVEKKKEEGGGEKKEKEG-GGGEKKEKEGGGGEKKEKEGGGGEKKEG-ESKPA-APAAAE 300
           AV    E GGGEKK++E  GGGEKKEKEGGG    EK+ GGGEKKE  ESKPA  PAAAE
Sbjct: 241 AVPVAVEAGGGEKKKEEADGGGEKKEKEGGG----EKKEGGGEKKEAVESKPAPPPAAAE 300

Query: 301 GGGAKAVEVINKMDYYGGYPYQQLYYEAPMQY-HSYSMEANPSYYNPNYGYVNHGYTDHR 360
           GGGAK V+V+NK++YYGGYPYQ LYYEAPMQY HSYSMEANPSYY PNYGY NHGYTDHR
Sbjct: 301 GGGAKPVDVMNKLEYYGGYPYQPLYYEAPMQYHHSYSMEANPSYYTPNYGYANHGYTDHR 360

Query: 361 YDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           YDVVPMD+HFPHHMQQ DQPQMFSDENPNACSVM
Sbjct: 361 YDVVPMDHHFPHHMQQ-DQPQMFSDENPNACSVM 389

BLAST of Spg033516 vs. NCBI nr
Match: XP_022982946.1 (heavy metal-associated isoprenylated plant protein 5-like [Cucurbita maxima])

HSP 1 Score: 544.3 bits (1401), Expect = 8.6e-151
Identity = 322/395 (81.52%), Postives = 338/395 (85.57%), Query Frame = 0

Query: 1   MGEQNVE---APPKAEGEKKPAA----APAPEPAAVPAPADGGAKKEGGGAITAIYKIDM 60
           MGEQ VE   A  K E EKK AA    APAP PAAVPA A+ GAKKE GGAITAIYKIDM
Sbjct: 1   MGEQKVEEVVAAAKNEVEKKSAAAVAPAPAPAPAAVPAVAEDGAKKEDGGAITAIYKIDM 60

Query: 61  HCEGCAKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQ 120
           HCEGCAKKIKRAVRHVKDVESV  DC ANKLTVIGKMDV+ VKEKLELKTKKKVE+ISPQ
Sbjct: 61  HCEGCAKKIKRAVRHVKDVESVKTDCAANKLTVIGKMDVVAVKEKLELKTKKKVEIISPQ 120

Query: 121 PKKDGGAAPA-GEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKING 180
           PKKDGGA  A  EKKA+EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIR+II KING
Sbjct: 121 PKKDGGAVAAPAEKKADEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRKIILKING 180

Query: 181 VQSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAA 240
           VQSV+ DGAKDLVT+KG MDVKQLEPYLKDKLKRNVEI+PPKKEEPA EKKKEE  A AA
Sbjct: 181 VQSVALDGAKDLVTVKGTMDVKQLEPYLKDKLKRNVEIIPPKKEEPAGEKKKEESPAPAA 240

Query: 241 PAAVEKKKEEGGGEKKEKEGGGGEKKEKEGGGGEKKEKEGGGGEKKEG-ESKPA-APAAA 300
             AV    E GGGEKKE+ GGGGEKKEKEGGG    EK+ GGGEKKE  ESKPA  PAA 
Sbjct: 241 VVAVPVAVEAGGGEKKEEAGGGGEKKEKEGGG----EKKEGGGEKKEAVESKPAPPPAAV 300

Query: 301 EGGGAKAVEVINKMDYYGGYPYQQLYYEAPMQY-HSYSMEANPSYYNPNYGYVNHGYTDH 360
           EGG AK V+V+NK++YYGGYPYQ LYYEAPMQY HSYSMEANPSYY PNYGY NHGYTDH
Sbjct: 301 EGGDAKPVDVMNKLEYYGGYPYQPLYYEAPMQYHHSYSMEANPSYYTPNYGYANHGYTDH 360

Query: 361 RYDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           RYDVVPMD+HFPHHMQQ +QPQMFSDENPNACSVM
Sbjct: 361 RYDVVPMDHHFPHHMQQ-NQPQMFSDENPNACSVM 390

BLAST of Spg033516 vs. ExPASy Swiss-Prot
Match: Q9SJL2 (Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=3702 GN=HIPP05 PE=1 SV=2)

HSP 1 Score: 242.7 bits (618), Expect = 7.0e-63
Identity = 195/406 (48.03%), Postives = 247/406 (60.84%), Query Frame = 0

Query: 1   MGEQNVEAPPKAEGEKKPAAAPAPEPAAVPAPADGGAKKEGGGA------ITA-IYKIDM 60
           MGE  V+  PK E EKKPAA   P       P  GG       A      ++A +YK+DM
Sbjct: 1   MGE--VQEGPKVEQEKKPAATVVPVETTDGKPKSGGGDSAAAAAPPVAAVVSAFVYKVDM 60

Query: 61  HCEGCAKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQ 120
           HCEGCAKKIKR V+H   V+ VTAD   NKL V+GK+D + ++EKLE KTK+KV L +P 
Sbjct: 61  HCEGCAKKIKRMVKHFDGVKDVTADTGGNKLLVVGKIDPVKLQEKLEEKTKRKVVLANPP 120

Query: 121 PKKDGG-AAPAGEKKAE--EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKI 180
           PK +G  AA  GEKKA+  +K+          PKES V LKIRLHCEGCIQKI++II KI
Sbjct: 121 PKVEGPVAAAVGEKKADGGDKEAAPPAPAPAAPKESVVPLKIRLHCEGCIQKIKKIILKI 180

Query: 181 NGVQSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVE-IVPPKKEEPAAEKKKEEPAA 240
            GV++V+ DGAKD+VT+KG +DVK+L P L  KLKR VE +VP KK++ AAE KK E AA
Sbjct: 181 KGVETVAIDGAKDVVTVKGTIDVKELVPLLTKKLKRTVEPLVPAKKDDGAAENKKTEAAA 240

Query: 241 -----AAAPAAVEKKKEEG--GGEKKEKEGGGGEKKEKEGGGGEKKEKEGGGGEKKEGES 300
                 A  A V + K+EG  GGEKK++ G GGEKK++ G GGEKK++ G GGEKK+   
Sbjct: 241 PDAKKEAPSAGVNEAKKEGSDGGEKKKEVGDGGEKKKEGGDGGEKKKEAGDGGEKKK--- 300

Query: 301 KPAAPAAAEGGGAKA-VEVINKMDYYG--GYPYQQLYYEAPMQY-HSYSMEANPSYYNPN 360
                   +GGG  A V ++NKMDYYG   YP   ++++    Y  SYSM         N
Sbjct: 301 --------DGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQEGHVYGQSYSMTGQ------N 360

Query: 361 YGYVNHGYTDHRYDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           Y      Y    Y+    +++ P+     + P MFSDENPN CSVM
Sbjct: 361 YPVGGQSYPGSGYNYA-SESYVPYAQPNVNAPGMFSDENPNGCSVM 386

BLAST of Spg033516 vs. ExPASy Swiss-Prot
Match: Q9LZF1 (Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=3702 GN=HIPP06 PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 4.0e-50
Identity = 178/432 (41.20%), Postives = 228/432 (52.78%), Query Frame = 0

Query: 1   MGEQNVEAPPKAEGEKKPAAAPAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHCEGCAK 60
           MGE+  E   K +GEKKP                       GG  T + K+DMHCEGC K
Sbjct: 1   MGEKKEETATKPQGEKKPT---------------------DGGITTVVMKLDMHCEGCGK 60

Query: 61  KIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELIS--PQPKKD- 120
           KIKR  +H K VE V  D  +NKLTVIG +D + V++K+  K K+ VEL+S    PKK+ 
Sbjct: 61  KIKRIFKHFKGVEDVKIDYKSNKLTVIGNVDPVEVRDKVADKIKRPVELVSTVAPPKKET 120

Query: 121 ----GGA----APAGEKKAEEKKP------------------EEKKAPEEKPKESTVVLK 180
               GGA    +PA E+K  EKKP                  E+K +P   PKESTVVLK
Sbjct: 121 PPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLK 180

Query: 181 IRLHCEGCIQKIRRIISKINGVQSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIV 240
            +LHCEGC  KI+RI++KI GV SV+ D AKDLV +KG +DVKQL PYL +KLKR VE+V
Sbjct: 181 TKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTPYLNEKLKRTVEVV 240

Query: 241 PPKKEEPAAEKKKEEPAAAAAPAAVEKKKEEGGGEKKEKEGGGGEKKEKEGGGGEKKEKE 300
           P KK++ A        AAAAAPA  EKK +  G +K+ K+ G   +K+ +GGG +KKE  
Sbjct: 241 PAKKDDGA-----PVAAAAAAPAGGEKKDKVAGEKKEIKDVG---EKKVDGGGEKKKEVA 300

Query: 301 GGGGEKKEGESKPAAPAAAEGGGAKAVEVINKMDYYG-GYPYQQLYYEAP-----MQYHS 360
            GGG    G           GGG      + K +Y G GYP Q +YY         Q++ 
Sbjct: 301 VGGGGGGGG-----------GGGDGGAMDVKKSEYNGYGYPPQPMYYYPEGQVYGQQHYM 360

Query: 361 YSMEANPSYYN---PNYGYVNHGYTDHRY-DVVPMDNHFPHHMQQHD---------QPQM 385
              +++ SY      N GYV   Y +  Y      +   P +M Q            P++
Sbjct: 361 MQGQSSQSYVQEPYSNQGYVQESYMNQGYGQGYGQEAPPPPYMNQQGYADPYGHMRAPEL 392

BLAST of Spg033516 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.3e-40
Identity = 139/365 (38.08%), Postives = 188/365 (51.51%), Query Frame = 0

Query: 34  DGGAKKEGGG--------AITAIYKIDMHCEGCAKKIKRAVRHVKDVESVTADCVANKLT 93
           +G  KK+GG         +IT + K+DMHCEGCA +I + VR  + VE+V ++    KLT
Sbjct: 7   EGDNKKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLT 66

Query: 94  VIGKMDVLVVKEKLELKTKKKVELISPQPKKDGGAAPAGEKKAEEKKPEEKKAP---EEK 153
           V G +D + ++EKLE KTKKKV+L+SPQPKK+       +   ++KK EEKK P   ++K
Sbjct: 67  VTGALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKK 126

Query: 154 PKE---STVVLKIRLHCEGCIQKIRRIISKINGVQSVSFDGAKDLVTIKGAMDVKQLEPY 213
           PKE   +T VLK+  HC+GCI KI++ ++K  GV  ++ D  K+L+T+KG MDVK+L   
Sbjct: 127 PKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEI 186

Query: 214 LKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAPAAVEKKKEEGGGEKKEKEGGGGEKKE 273
           L +KLKR VEIVPPKK                     EK KE G    ++K+GGGG    
Sbjct: 187 LSEKLKRAVEIVPPKK---------------------EKDKENGNENGEKKKGGGG---- 246

Query: 274 KEGGGGEKKEKEGGGGEKKEGESKPAAPAAAEGGGAKAVEVINKMDYYGGYPYQQLYYEA 333
            +GGG EK   +GGG                        E +N M+Y             
Sbjct: 247 -DGGGKEKTGNKGGG------------------------EGVNMMEY------------- 283

Query: 334 PMQYHSYSMEANPSYYNPNYGYVNHGYTDHRYDVVPMDNHFPHHMQQHDQPQMFSDENPN 385
                   M A P+Y    YGY   G              + + +Q H  PQ+FSDENPN
Sbjct: 307 --------MAAQPAY---GYGYYPGG-------------PYGYPIQAH-APQIFSDENPN 283

BLAST of Spg033516 vs. ExPASy Swiss-Prot
Match: Q9C5D3 (Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=3702 GN=HIPP07 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.6e-25
Identity = 136/392 (34.69%), Postives = 187/392 (47.70%), Query Frame = 0

Query: 2   GEQNVEAPPKAEG---EKKPAAAPAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHCEGC 61
           GE + + P + E     K+ +A  APE  A P P               + K+ MHCEGC
Sbjct: 38  GEDSDKKPQEGESNKDSKEDSAPAAPEAPAPPPPPQ-----------EVVLKVYMHCEGC 97

Query: 62  AKKIKRAVRHVKDVESVTADCVANKLTVIG-KMDVLVVKEKLELKTKKKVELISPQPKKD 121
           A+K++R ++  + VE V  DC   K+ V G K D L V  +++ KT ++V+L+SP P   
Sbjct: 98  ARKVRRCLKGFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSPIPP-- 157

Query: 122 GGAAPAGEKKAEEKKP--EEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKINGVQS 181
               P  EKKAEE KP  EEKK   E P   TVVLK+ +HCE C  +I++ I ++ GV+S
Sbjct: 158 --PPPPPEKKAEEDKPIVEEKKV--EPPVVVTVVLKVHMHCEACATEIKKRIMRMKGVES 217

Query: 182 VSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAPAA 241
              D     VT+KG  + ++L  Y+  +  ++  I+   K +P      EE AAAA    
Sbjct: 218 AESDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIM---KIDPPPPPPPEEAAAAAE--- 277

Query: 242 VEKKKEEGGGEKKEKEGGGGEKKEKEGGGGEKK---EKEGGGGEKKEGESKPAAPAAAEG 301
                    GEKKE+E G GE K +EG   + K   EK+ G G K EGE      AA  G
Sbjct: 278 ---------GEKKEEEKGEGESKGEEGKDDKAKTDEEKKEGDGGKGEGE------AADNG 337

Query: 302 GGAKAVEVINKMDYYGGYPYQQLYYEAPMQYHSYSMEANPSYYNPNYGYVNHGYTDHRYD 361
           GG +  +V+         PY   YY+ P                             R  
Sbjct: 338 GGEEEGKVVEVRKIEN--PYYYYYYQPP-----------------------------RVA 355

Query: 362 VVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           + PM+   P H      PQ+FSDENPNAC+VM
Sbjct: 398 IPPME--MPPHAY---PPQLFSDENPNACTVM 355

BLAST of Spg033516 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 8.4e-24
Identity = 83/209 (39.71%), Postives = 120/209 (57.42%), Query Frame = 0

Query: 45  ITAIYKIDMHCEGCAKKIKRAVRHVKDVESVTADCVANKLTVIG-KMDVLVVKEKLELKT 104
           +  + KID HC+GC  +I R  R ++ VE+V AD  +NKLT+IG  MD + + EKL+ K+
Sbjct: 29  VHVVLKIDFHCDGCIARIVRLSRRLEGVETVRADPDSNKLTLIGFIMDPVKIAEKLQKKS 88

Query: 105 KKKVELISPQPKKDGGAAPAGEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKI 164
           KKKVELISP+PKKD        K+  EKK  +K   +     +TVVLK+   C+GCI++I
Sbjct: 89  KKKVELISPKPKKD-------TKENNEKKANDK--TQTVVAVTTVVLKVNCSCDGCIKRI 148

Query: 165 RRIISKINGVQSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKK 224
           ++ +S   GV  V  D  K+ VT+ G MD+K +   LK KLK+ V++VP KK++   +  
Sbjct: 149 QKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQVVPEKKKKKKDKDN 208

Query: 225 KEEPAAAAAPAAVEKKKEEGGGEKKEKEG 253
            E      +P         G G  +  EG
Sbjct: 209 AEVNTKVGSPCQPGNGCTHGIGPYRFMEG 228

BLAST of Spg033516 vs. ExPASy TrEMBL
Match: A0A5D3DNF6 (Heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002700 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 5.2e-154
Identity = 330/395 (83.54%), Postives = 337/395 (85.32%), Query Frame = 0

Query: 1   MGEQNVEAP-PKAEGEKKPAA-APAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHCEGC 60
           MGE  VE P  K E EKKPAA  PAP PAAVP   DGGAKKE GGAITAIYKIDMHCEGC
Sbjct: 1   MGEAKVEEPAAKNESEKKPAAPVPAPAPAAVPVATDGGAKKEDGGAITAIYKIDMHCEGC 60

Query: 61  AKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQPKKDG 120
           AKKIKRAVRHVKDVESV ADC ANKLTVIGKMDV+ VK+KLELKTKKKVELISPQPKKD 
Sbjct: 61  AKKIKRAVRHVKDVESVKADCGANKLTVIGKMDVVAVKQKLELKTKKKVELISPQPKKDA 120

Query: 121 GAA-----PAGEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKINGV 180
            AA      A EKKA+EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRII KINGV
Sbjct: 121 PAAAAAAPAAAEKKADEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIILKINGV 180

Query: 181 QSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAP 240
            SV  DG KDLVT+KG MDVKQLEPYLKDKLKRNVEIVPPKKEEPA EKKKEE  A AAP
Sbjct: 181 HSVDLDGVKDLVTVKGTMDVKQLEPYLKDKLKRNVEIVPPKKEEPAGEKKKEESPAPAAP 240

Query: 241 A-AVEKKKEE--GGGEKKEKEGGGGEKKEKEGGGGEKKEKEGGGGEKKEGESKPAAPAAA 300
           A   EKKKEE  GGGEKKEKEGGGGEK  KE GGGEKKEKE GGGEKKEGESKPA P AA
Sbjct: 241 AGGGEKKKEEAGGGGEKKEKEGGGGEK--KEAGGGEKKEKE-GGGEKKEGESKPAPPPAA 300

Query: 301 EGGGAKAVEVINKMDYYGGYPYQQLYYEAPMQYHSYSMEANPS-YYNPNYGYVNHGYTDH 360
           EGGGAK  EV+NK +YYGGYPYQ LYYEAPMQY SYSMEANPS YYN NYGY NHGY DH
Sbjct: 301 EGGGAKPAEVVNKFEYYGGYPYQPLYYEAPMQYQSYSMEANPSYYYNHNYGYNNHGYVDH 360

Query: 361 RYDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           RYDVVPMD HFPHHM Q+DQPQMFSDENPNACSVM
Sbjct: 361 RYDVVPMDPHFPHHM-QNDQPQMFSDENPNACSVM 391

BLAST of Spg033516 vs. ExPASy TrEMBL
Match: A0A1S3B6C2 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=3656 GN=LOC103486498 PE=4 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 5.2e-154
Identity = 330/395 (83.54%), Postives = 337/395 (85.32%), Query Frame = 0

Query: 1   MGEQNVEAP-PKAEGEKKPAA-APAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHCEGC 60
           MGE  VE P  K E EKKPAA  PAP PAAVP   DGGAKKE GGAITAIYKIDMHCEGC
Sbjct: 1   MGEAKVEEPAAKNESEKKPAAPVPAPAPAAVPVATDGGAKKEDGGAITAIYKIDMHCEGC 60

Query: 61  AKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQPKKDG 120
           AKKIKRAVRHVKDVESV ADC ANKLTVIGKMDV+ VK+KLELKTKKKVELISPQPKKD 
Sbjct: 61  AKKIKRAVRHVKDVESVKADCGANKLTVIGKMDVVAVKQKLELKTKKKVELISPQPKKDA 120

Query: 121 GAA-----PAGEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKINGV 180
            AA      A EKKA+EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRII KINGV
Sbjct: 121 PAAAAAAPAAAEKKADEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIILKINGV 180

Query: 181 QSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAP 240
            SV  DG KDLVT+KG MDVKQLEPYLKDKLKRNVEIVPPKKEEPA EKKKEE  A AAP
Sbjct: 181 HSVDLDGVKDLVTVKGTMDVKQLEPYLKDKLKRNVEIVPPKKEEPAGEKKKEESPAPAAP 240

Query: 241 A-AVEKKKEE--GGGEKKEKEGGGGEKKEKEGGGGEKKEKEGGGGEKKEGESKPAAPAAA 300
           A   EKKKEE  GGGEKKEKEGGGGEK  KE GGGEKKEKE GGGEKKEGESKPA P AA
Sbjct: 241 AGGGEKKKEEAGGGGEKKEKEGGGGEK--KEAGGGEKKEKE-GGGEKKEGESKPAPPPAA 300

Query: 301 EGGGAKAVEVINKMDYYGGYPYQQLYYEAPMQYHSYSMEANPS-YYNPNYGYVNHGYTDH 360
           EGGGAK  EV+NK +YYGGYPYQ LYYEAPMQY SYSMEANPS YYN NYGY NHGY DH
Sbjct: 301 EGGGAKPAEVVNKFEYYGGYPYQPLYYEAPMQYQSYSMEANPSYYYNHNYGYNNHGYVDH 360

Query: 361 RYDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           RYDVVPMD HFPHHM Q+DQPQMFSDENPNACSVM
Sbjct: 361 RYDVVPMDPHFPHHM-QNDQPQMFSDENPNACSVM 391

BLAST of Spg033516 vs. ExPASy TrEMBL
Match: A0A6J1F943 (heavy metal-associated isoprenylated plant protein 5-like OS=Cucurbita moschata OX=3662 GN=LOC111441960 PE=4 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.4e-151
Identity = 323/394 (81.98%), Postives = 340/394 (86.29%), Query Frame = 0

Query: 1   MGEQNVE---APPKAEGEKKPAA--APAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHC 60
           MGEQ VE   A  K E EKK AA  APAP PAAVPA A+GGAKKE GGAITA+YKIDMHC
Sbjct: 1   MGEQKVEEAVAAAKNEVEKKSAAAVAPAPAPAAVPAVAEGGAKKEDGGAITAVYKIDMHC 60

Query: 61  EGCAKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQPK 120
           EGCAKKIKR VRHVKDVESV  DC ANKLTVIGKMDV+ VKEKLELKTKKKVELISPQPK
Sbjct: 61  EGCAKKIKRTVRHVKDVESVKTDCGANKLTVIGKMDVVAVKEKLELKTKKKVELISPQPK 120

Query: 121 KDGGAAPA-GEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKINGVQ 180
           KDGGA  A  EKKA+EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIR+II KINGVQ
Sbjct: 121 KDGGAVAAPAEKKADEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRKIILKINGVQ 180

Query: 181 SVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAPA 240
           SV+ DGAKDLVT+KG MDVKQLEPYLKDKLKRNVE++PPKKEEPA EKKKEE  A AA  
Sbjct: 181 SVALDGAKDLVTVKGTMDVKQLEPYLKDKLKRNVEVIPPKKEEPAGEKKKEESPAPAAVV 240

Query: 241 AVEKKKEEGGGEKKEKEG-GGGEKKEKEGGGGEKKEKEGGGGEKKEG-ESKPA-APAAAE 300
           AV    E GGGEKK++E  GGGEKKEKEGGG    EK+ GGGEKKE  ESKPA  PAAAE
Sbjct: 241 AVPVAVEAGGGEKKKEEADGGGEKKEKEGGG----EKKEGGGEKKEAVESKPAPPPAAAE 300

Query: 301 GGGAKAVEVINKMDYYGGYPYQQLYYEAPMQY-HSYSMEANPSYYNPNYGYVNHGYTDHR 360
           GGGAK V+V+NK++YYGGYPYQ LYYEAPMQY HSYSMEANPSYY PNYGY NHGYTDHR
Sbjct: 301 GGGAKPVDVMNKLEYYGGYPYQPLYYEAPMQYHHSYSMEANPSYYTPNYGYANHGYTDHR 360

Query: 361 YDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           YDVVPMD+HFPHHMQQ DQPQMFSDENPNACSVM
Sbjct: 361 YDVVPMDHHFPHHMQQ-DQPQMFSDENPNACSVM 389

BLAST of Spg033516 vs. ExPASy TrEMBL
Match: A0A6J1J0S3 (heavy metal-associated isoprenylated plant protein 5-like OS=Cucurbita maxima OX=3661 GN=LOC111481639 PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 4.2e-151
Identity = 322/395 (81.52%), Postives = 338/395 (85.57%), Query Frame = 0

Query: 1   MGEQNVE---APPKAEGEKKPAA----APAPEPAAVPAPADGGAKKEGGGAITAIYKIDM 60
           MGEQ VE   A  K E EKK AA    APAP PAAVPA A+ GAKKE GGAITAIYKIDM
Sbjct: 1   MGEQKVEEVVAAAKNEVEKKSAAAVAPAPAPAPAAVPAVAEDGAKKEDGGAITAIYKIDM 60

Query: 61  HCEGCAKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQ 120
           HCEGCAKKIKRAVRHVKDVESV  DC ANKLTVIGKMDV+ VKEKLELKTKKKVE+ISPQ
Sbjct: 61  HCEGCAKKIKRAVRHVKDVESVKTDCAANKLTVIGKMDVVAVKEKLELKTKKKVEIISPQ 120

Query: 121 PKKDGGAAPA-GEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKING 180
           PKKDGGA  A  EKKA+EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIR+II KING
Sbjct: 121 PKKDGGAVAAPAEKKADEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRKIILKING 180

Query: 181 VQSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAA 240
           VQSV+ DGAKDLVT+KG MDVKQLEPYLKDKLKRNVEI+PPKKEEPA EKKKEE  A AA
Sbjct: 181 VQSVALDGAKDLVTVKGTMDVKQLEPYLKDKLKRNVEIIPPKKEEPAGEKKKEESPAPAA 240

Query: 241 PAAVEKKKEEGGGEKKEKEGGGGEKKEKEGGGGEKKEKEGGGGEKKEG-ESKPA-APAAA 300
             AV    E GGGEKKE+ GGGGEKKEKEGGG    EK+ GGGEKKE  ESKPA  PAA 
Sbjct: 241 VVAVPVAVEAGGGEKKEEAGGGGEKKEKEGGG----EKKEGGGEKKEAVESKPAPPPAAV 300

Query: 301 EGGGAKAVEVINKMDYYGGYPYQQLYYEAPMQY-HSYSMEANPSYYNPNYGYVNHGYTDH 360
           EGG AK V+V+NK++YYGGYPYQ LYYEAPMQY HSYSMEANPSYY PNYGY NHGYTDH
Sbjct: 301 EGGDAKPVDVMNKLEYYGGYPYQPLYYEAPMQYHHSYSMEANPSYYTPNYGYANHGYTDH 360

Query: 361 RYDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           RYDVVPMD+HFPHHMQQ +QPQMFSDENPNACSVM
Sbjct: 361 RYDVVPMDHHFPHHMQQ-NQPQMFSDENPNACSVM 390

BLAST of Spg033516 vs. ExPASy TrEMBL
Match: A0A0A0LDJ4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740220 PE=4 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 3.3e-148
Identity = 322/398 (80.90%), Postives = 335/398 (84.17%), Query Frame = 0

Query: 1   MGEQNV-EAPPKAEGEKKPAA-APAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHCEGC 60
           MGE  V E+  K E EKKPAA  PAP PAAVP   DGGAKKE GGA+TAIYKIDMHCEGC
Sbjct: 1   MGEAKVEESAAKNEAEKKPAAPVPAPAPAAVPVVKDGGAKKEEGGAVTAIYKIDMHCEGC 60

Query: 61  AKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQPKKDG 120
           AKKIKRAVRHVKDVESV ADC ANKLTVIG+MDV+ VK+KLELKTKKKVELISPQPKKD 
Sbjct: 61  AKKIKRAVRHVKDVESVKADCGANKLTVIGRMDVVAVKQKLELKTKKKVELISPQPKKDA 120

Query: 121 GAAPAGEKKAEEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKINGVQSVSF 180
            AA A    A EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRII KINGVQSV  
Sbjct: 121 PAAAAAAPAAAEKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIILKINGVQSVDL 180

Query: 181 DGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIVPPKKEEPAAEKKKEE-PAAAAAPAAVE 240
           DG KDLVT+KG MDVKQLEPYLKDKLKR VEIVPPKKEE A EK KEE PA AAA    E
Sbjct: 181 DGVKDLVTVKGTMDVKQLEPYLKDKLKRKVEIVPPKKEEAAGEKTKEESPAPAAAAGGGE 240

Query: 241 KKKEE--GGGEKKEKEGGGGEKKEKEGGGGEKKEKEGGGGEKKEGESKP---AAPAAA-E 300
           KKKEE  GGGEKKEKEGGGGEK  KEGGGGE+KEKE GGGEKKEGESKP   AAPAAA E
Sbjct: 241 KKKEEAGGGGEKKEKEGGGGEK--KEGGGGEQKEKE-GGGEKKEGESKPAPAAAPAAAGE 300

Query: 301 GGGAKAVEVINKMDYYGGYPYQQLYYEAPMQYHSYSMEANPSYYNPNY-----GYVNHGY 360
           GGG K  +V+NK +YYGGYP+QQLYYEAPMQY SYSMEANPSYYNPNY     GY NHGY
Sbjct: 301 GGGVKPADVVNKFEYYGGYPFQQLYYEAPMQYQSYSMEANPSYYNPNYGYNNHGYDNHGY 360

Query: 361 TDHRYDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
            DHRYDVVPMD HFPHHM ++DQPQMFSDENPNACSVM
Sbjct: 361 VDHRYDVVPMDPHFPHHM-RNDQPQMFSDENPNACSVM 394

BLAST of Spg033516 vs. TAIR 10
Match: AT2G36950.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 5.0e-64
Identity = 195/406 (48.03%), Postives = 247/406 (60.84%), Query Frame = 0

Query: 1   MGEQNVEAPPKAEGEKKPAAAPAPEPAAVPAPADGGAKKEGGGA------ITA-IYKIDM 60
           MGE  V+  PK E EKKPAA   P       P  GG       A      ++A +YK+DM
Sbjct: 1   MGE--VQEGPKVEQEKKPAATVVPVETTDGKPKSGGGDSAAAAAPPVAAVVSAFVYKVDM 60

Query: 61  HCEGCAKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELISPQ 120
           HCEGCAKKIKR V+H   V+ VTAD   NKL V+GK+D + ++EKLE KTK+KV L +P 
Sbjct: 61  HCEGCAKKIKRMVKHFDGVKDVTADTGGNKLLVVGKIDPVKLQEKLEEKTKRKVVLANPP 120

Query: 121 PKKDGG-AAPAGEKKAE--EKKPEEKKAPEEKPKESTVVLKIRLHCEGCIQKIRRIISKI 180
           PK +G  AA  GEKKA+  +K+          PKES V LKIRLHCEGCIQKI++II KI
Sbjct: 121 PKVEGPVAAAVGEKKADGGDKEAAPPAPAPAAPKESVVPLKIRLHCEGCIQKIKKIILKI 180

Query: 181 NGVQSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVE-IVPPKKEEPAAEKKKEEPAA 240
            GV++V+ DGAKD+VT+KG +DVK+L P L  KLKR VE +VP KK++ AAE KK E AA
Sbjct: 181 KGVETVAIDGAKDVVTVKGTIDVKELVPLLTKKLKRTVEPLVPAKKDDGAAENKKTEAAA 240

Query: 241 -----AAAPAAVEKKKEEG--GGEKKEKEGGGGEKKEKEGGGGEKKEKEGGGGEKKEGES 300
                 A  A V + K+EG  GGEKK++ G GGEKK++ G GGEKK++ G GGEKK+   
Sbjct: 241 PDAKKEAPSAGVNEAKKEGSDGGEKKKEVGDGGEKKKEGGDGGEKKKEAGDGGEKKK--- 300

Query: 301 KPAAPAAAEGGGAKA-VEVINKMDYYG--GYPYQQLYYEAPMQY-HSYSMEANPSYYNPN 360
                   +GGG  A V ++NKMDYYG   YP   ++++    Y  SYSM         N
Sbjct: 301 --------DGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQEGHVYGQSYSMTGQ------N 360

Query: 361 YGYVNHGYTDHRYDVVPMDNHFPHHMQQHDQPQMFSDENPNACSVM 385
           Y      Y    Y+    +++ P+     + P MFSDENPN CSVM
Sbjct: 361 YPVGGQSYPGSGYNYA-SESYVPYAQPNVNAPGMFSDENPNGCSVM 386

BLAST of Spg033516 vs. TAIR 10
Match: AT5G03380.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 200.3 bits (508), Expect = 2.8e-51
Identity = 178/432 (41.20%), Postives = 228/432 (52.78%), Query Frame = 0

Query: 1   MGEQNVEAPPKAEGEKKPAAAPAPEPAAVPAPADGGAKKEGGGAITAIYKIDMHCEGCAK 60
           MGE+  E   K +GEKKP                       GG  T + K+DMHCEGC K
Sbjct: 1   MGEKKEETATKPQGEKKPT---------------------DGGITTVVMKLDMHCEGCGK 60

Query: 61  KIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVELIS--PQPKKD- 120
           KIKR  +H K VE V  D  +NKLTVIG +D + V++K+  K K+ VEL+S    PKK+ 
Sbjct: 61  KIKRIFKHFKGVEDVKIDYKSNKLTVIGNVDPVEVRDKVADKIKRPVELVSTVAPPKKET 120

Query: 121 ----GGA----APAGEKKAEEKKP------------------EEKKAPEEKPKESTVVLK 180
               GGA    +PA E+K  EKKP                  E+K +P   PKESTVVLK
Sbjct: 121 PPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLK 180

Query: 181 IRLHCEGCIQKIRRIISKINGVQSVSFDGAKDLVTIKGAMDVKQLEPYLKDKLKRNVEIV 240
            +LHCEGC  KI+RI++KI GV SV+ D AKDLV +KG +DVKQL PYL +KLKR VE+V
Sbjct: 181 TKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTPYLNEKLKRTVEVV 240

Query: 241 PPKKEEPAAEKKKEEPAAAAAPAAVEKKKEEGGGEKKEKEGGGGEKKEKEGGGGEKKEKE 300
           P KK++ A        AAAAAPA  EKK +  G +K+ K+ G   +K+ +GGG +KKE  
Sbjct: 241 PAKKDDGA-----PVAAAAAAPAGGEKKDKVAGEKKEIKDVG---EKKVDGGGEKKKEVA 300

Query: 301 GGGGEKKEGESKPAAPAAAEGGGAKAVEVINKMDYYG-GYPYQQLYYEAP-----MQYHS 360
            GGG    G           GGG      + K +Y G GYP Q +YY         Q++ 
Sbjct: 301 VGGGGGGGG-----------GGGDGGAMDVKKSEYNGYGYPPQPMYYYPEGQVYGQQHYM 360

Query: 361 YSMEANPSYYN---PNYGYVNHGYTDHRY-DVVPMDNHFPHHMQQHD---------QPQM 385
              +++ SY      N GYV   Y +  Y      +   P +M Q            P++
Sbjct: 361 MQGQSSQSYVQEPYSNQGYVQESYMNQGYGQGYGQEAPPPPYMNQQGYADPYGHMRAPEL 392

BLAST of Spg033516 vs. TAIR 10
Match: AT5G03380.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.7e-48
Identity = 165/383 (43.08%), Postives = 212/383 (55.35%), Query Frame = 0

Query: 50  KIDMHCEGCAKKIKRAVRHVKDVESVTADCVANKLTVIGKMDVLVVKEKLELKTKKKVEL 109
           K+DMHCEGC KKIKR  +H K VE V  D  +NKLTVIG +D + V++K+  K K+ VEL
Sbjct: 2   KLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIGNVDPVEVRDKVADKIKRPVEL 61

Query: 110 IS--PQPKKD-----GGA----APAGEKKAEEKKP------------------EEKKAPE 169
           +S    PKK+     GGA    +PA E+K  EKKP                  E+K +P 
Sbjct: 62  VSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKASPP 121

Query: 170 EKPKESTVVLKIRLHCEGCIQKIRRIISKINGVQSVSFDGAKDLVTIKGAMDVKQLEPYL 229
             PKESTVVLK +LHCEGC  KI+RI++KI GV SV+ D AKDLV +KG +DVKQL PYL
Sbjct: 122 PPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTPYL 181

Query: 230 KDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAPAAVEKKKEEGGGEKKEKEGGGGEKKEK 289
            +KLKR VE+VP KK++ A        AAAAAPA  EKK +  G +K+ K+ G   +K+ 
Sbjct: 182 NEKLKRTVEVVPAKKDDGA-----PVAAAAAAPAGGEKKDKVAGEKKEIKDVG---EKKV 241

Query: 290 EGGGGEKKEKEGGGGEKKEGESKPAAPAAAEGGGAKAVEVINKMDYYG-GYPYQQLYYEA 349
           +GGG +KKE   GGG    G           GGG      + K +Y G GYP Q +YY  
Sbjct: 242 DGGGEKKKEVAVGGGGGGGG-----------GGGDGGAMDVKKSEYNGYGYPPQPMYYYP 301

Query: 350 P-----MQYHSYSMEANPSYYN---PNYGYVNHGYTDHRY-DVVPMDNHFPHHMQQHD-- 385
                  Q++    +++ SY      N GYV   Y +  Y      +   P +M Q    
Sbjct: 302 EGQVYGQQHYMMQGQSSQSYVQEPYSNQGYVQESYMNQGYGQGYGQEAPPPPYMNQQGYA 361

BLAST of Spg033516 vs. TAIR 10
Match: AT5G60800.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 168.7 bits (426), Expect = 9.1e-42
Identity = 139/365 (38.08%), Postives = 188/365 (51.51%), Query Frame = 0

Query: 34  DGGAKKEGGG--------AITAIYKIDMHCEGCAKKIKRAVRHVKDVESVTADCVANKLT 93
           +G  KK+GG         +IT + K+DMHCEGCA +I + VR  + VE+V ++    KLT
Sbjct: 7   EGDNKKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLT 66

Query: 94  VIGKMDVLVVKEKLELKTKKKVELISPQPKKDGGAAPAGEKKAEEKKPEEKKAP---EEK 153
           V G +D + ++EKLE KTKKKV+L+SPQPKK+       +   ++KK EEKK P   ++K
Sbjct: 67  VTGALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKK 126

Query: 154 PKE---STVVLKIRLHCEGCIQKIRRIISKINGVQSVSFDGAKDLVTIKGAMDVKQLEPY 213
           PKE   +T VLK+  HC+GCI KI++ ++K  GV  ++ D  K+L+T+KG MDVK+L   
Sbjct: 127 PKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEI 186

Query: 214 LKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAPAAVEKKKEEGGGEKKEKEGGGGEKKE 273
           L +KLKR VEIVPPKK                     EK KE G    ++K+GGGG    
Sbjct: 187 LSEKLKRAVEIVPPKK---------------------EKDKENGNENGEKKKGGGG---- 246

Query: 274 KEGGGGEKKEKEGGGGEKKEGESKPAAPAAAEGGGAKAVEVINKMDYYGGYPYQQLYYEA 333
            +GGG EK   +GGG                        E +N M+Y             
Sbjct: 247 -DGGGKEKTGNKGGG------------------------EGVNMMEY------------- 283

Query: 334 PMQYHSYSMEANPSYYNPNYGYVNHGYTDHRYDVVPMDNHFPHHMQQHDQPQMFSDENPN 385
                   M A P+Y    YGY   G              + + +Q H  PQ+FSDENPN
Sbjct: 307 --------MAAQPAY---GYGYYPGG-------------PYGYPIQAH-APQIFSDENPN 283

BLAST of Spg033516 vs. TAIR 10
Match: AT5G60800.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 2.7e-41
Identity = 138/365 (37.81%), Postives = 188/365 (51.51%), Query Frame = 0

Query: 34  DGGAKKEGGG--------AITAIYKIDMHCEGCAKKIKRAVRHVKDVESVTADCVANKLT 93
           +G  KK+GG         +IT + K+DMHCEGCA +I + VR  + VE+V ++    KLT
Sbjct: 7   EGDNKKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLT 66

Query: 94  VIGKMDVLVVKEKLELKTKKKVELISPQPKKDGGAAPAGEKKAEEKKPEEKKAP---EEK 153
           V G +D + ++EKLE KTKKKV+L+SPQPKK+       +   ++KK EEKK P   ++K
Sbjct: 67  VTGALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKK 126

Query: 154 PKE---STVVLKIRLHCEGCIQKIRRIISKINGVQSVSFDGAKDLVTIKGAMDVKQLEPY 213
           PKE   +T VLK+  HC+GCI KI++ ++K  GV  ++ D  K+L+T+KG MDVK+L   
Sbjct: 127 PKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEI 186

Query: 214 LKDKLKRNVEIVPPKKEEPAAEKKKEEPAAAAAPAAVEKKKEEGGGEKKEKEGGGGEKKE 273
           L +KLKR VEIVPPKK                     EK KE G    ++K+GGGG    
Sbjct: 187 LSEKLKRAVEIVPPKK---------------------EKDKENGNENGEKKKGGGG---- 246

Query: 274 KEGGGGEKKEKEGGGGEKKEGESKPAAPAAAEGGGAKAVEVINKMDYYGGYPYQQLYYEA 333
            +GGG EK   +GGG                        E +N M+Y             
Sbjct: 247 -DGGGKEKTGNKGGG------------------------EGVNMMEY------------- 283

Query: 334 PMQYHSYSMEANPSYYNPNYGYVNHGYTDHRYDVVPMDNHFPHHMQQHDQPQMFSDENPN 385
                   M A P+Y    YGY   G              + + +Q H  PQ+FSDENPN
Sbjct: 307 --------MAAQPAY---GYGYYPGG-------------PYGYPIQAH-APQIFSDENPN 283