Spg033503 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg033503
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	RING-type domain-containing protein
Location	scaffold5: 2313680 .. 2314528 (+)
RNA-Seq Expression	Spg033503
Synteny	Spg033503

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCCCTCTCCGCCCTCTCTCCGCCGCATCTCTCCGATCTCTCCCACTCCATCTTCTCCGACATCCACCACCGCCGCCGCCGCCTCACCTTCATCCTCTCCTCCCCAACCCTCTTCTCCCTCACTCTCCGCCACCTCAATTCCCTCTCCCTCTCCCACAAATCTCTCCTCATCGCCCGCTTCCTTCTCTCCGCCCTCCGCCGCCTCTCCCGCCCCTTCCAGCCGCCGTCCAAGTCCCTCCCCCACCACCCCTCCACCGCCGCCGTCACCCCCCGGGACCTCGACGCCGCTGTCCTTCTCCTCCTCCTCTGCGAGGTCCGACAGCACAGTCCCACCGCCCTCCAAACTCCGATCACCAAATGGCGATCGACCCTCTGCAGAATCTACTCCGATTCCCTCTTGACGGTCTCCGGCGTCGCCACCGGCGGCGCCGGCGTTCTTATTCCGTACATTGAGACGGTGGCGAGGTGCTGGAAGTTCGTGGGGTTCGTCGGGAGCTGCAGCGGCGGGAAGGCCCGGAGAGAGGTGGCGGCGTCGCCGGTGGCGGTGGTGGAGCTGCCGTCGGTGGCGGTGGGCGGCGGGGCGGCGGTGGAATGTGTGATTTGTAAAGAGGAGATGAACGAAGGGAGAGACGCGTGTCAATTGCCTTGTGAACACTTATTCCATTGGCTCTGTATTCTGCCATGGCTGAGGAAGAGGAACACGTGTCCCTGTTGCAGGTTTCAGCTTCCCACCGATGACATTTTCGGCGAGATCCAACGGCTCTGGGAGATCCTCCTCAAGGTGGGCTCCACGTCTGATGGAGATCAACGTCCAGGATTGAATGGTCGCATGGAATTAGGATAG

mRNA sequence

ATGGCCGCCCTCTCCGCCCTCTCTCCGCCGCATCTCTCCGATCTCTCCCACTCCATCTTCTCCGACATCCACCACCGCCGCCGCCGCCTCACCTTCATCCTCTCCTCCCCAACCCTCTTCTCCCTCACTCTCCGCCACCTCAATTCCCTCTCCCTCTCCCACAAATCTCTCCTCATCGCCCGCTTCCTTCTCTCCGCCCTCCGCCGCCTCTCCCGCCCCTTCCAGCCGCCGTCCAAGTCCCTCCCCCACCACCCCTCCACCGCCGCCGTCACCCCCCGGGACCTCGACGCCGCTGTCCTTCTCCTCCTCCTCTGCGAGGTCCGACAGCACAGTCCCACCGCCCTCCAAACTCCGATCACCAAATGGCGATCGACCCTCTGCAGAATCTACTCCGATTCCCTCTTGACGGTCTCCGGCGTCGCCACCGGCGGCGCCGGCGTTCTTATTCCGTACATTGAGACGGTGGCGAGGTGCTGGAAGTTCGTGGGGTTCGTCGGGAGCTGCAGCGGCGGGAAGGCCCGGAGAGAGGTGGCGGCGTCGCCGGTGGCGGTGGTGGAGCTGCCGTCGGTGGCGGTGGGCGGCGGGGCGGCGGTGGAATGTGTGATTTGTAAAGAGGAGATGAACGAAGGGAGAGACGCGTGTCAATTGCCTTGTGAACACTTATTCCATTGGCTCTGTATTCTGCCATGGCTGAGGAAGAGGAACACGTGTCCCTGTTGCAGGTTTCAGCTTCCCACCGATGACATTTTCGGCGAGATCCAACGGCTCTGGGAGATCCTCCTCAAGGTGGGCTCCACGTCTGATGGAGATCAACGTCCAGGATTGAATGGTCGCATGGAATTAGGATAG

Coding sequence (CDS)

ATGGCCGCCCTCTCCGCCCTCTCTCCGCCGCATCTCTCCGATCTCTCCCACTCCATCTTCTCCGACATCCACCACCGCCGCCGCCGCCTCACCTTCATCCTCTCCTCCCCAACCCTCTTCTCCCTCACTCTCCGCCACCTCAATTCCCTCTCCCTCTCCCACAAATCTCTCCTCATCGCCCGCTTCCTTCTCTCCGCCCTCCGCCGCCTCTCCCGCCCCTTCCAGCCGCCGTCCAAGTCCCTCCCCCACCACCCCTCCACCGCCGCCGTCACCCCCCGGGACCTCGACGCCGCTGTCCTTCTCCTCCTCCTCTGCGAGGTCCGACAGCACAGTCCCACCGCCCTCCAAACTCCGATCACCAAATGGCGATCGACCCTCTGCAGAATCTACTCCGATTCCCTCTTGACGGTCTCCGGCGTCGCCACCGGCGGCGCCGGCGTTCTTATTCCGTACATTGAGACGGTGGCGAGGTGCTGGAAGTTCGTGGGGTTCGTCGGGAGCTGCAGCGGCGGGAAGGCCCGGAGAGAGGTGGCGGCGTCGCCGGTGGCGGTGGTGGAGCTGCCGTCGGTGGCGGTGGGCGGCGGGGCGGCGGTGGAATGTGTGATTTGTAAAGAGGAGATGAACGAAGGGAGAGACGCGTGTCAATTGCCTTGTGAACACTTATTCCATTGGCTCTGTATTCTGCCATGGCTGAGGAAGAGGAACACGTGTCCCTGTTGCAGGTTTCAGCTTCCCACCGATGACATTTTCGGCGAGATCCAACGGCTCTGGGAGATCCTCCTCAAGGTGGGCTCCACGTCTGATGGAGATCAACGTCCAGGATTGAATGGTCGCATGGAATTAGGATAG

Protein sequence

MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIARFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPITKWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAASPVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCCRFQLPTDDIFGEIQRLWEILLKVGSTSDGDQRPGLNGRMELG

Homology

[Close]

BLAST of Spg033503 vs. NCBI nr
Match: KAG6596438.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 501.1 bits (1289), Expect = 6.1e-138
Identity = 246/270 (91.11%), Postives = 257/270 (95.19%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAALS LSPPHLSDLSHSIFSD+H  RRRLTFILSSPTLFS TLRHLNSLSLSHKSLLIA
Sbjct: 11  MAALSTLSPPHLSDLSHSIFSDVHTSRRRLTFILSSPTLFSFTLRHLNSLSLSHKSLLIA 70

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRLSRPFQ PSKS PHHPSTAAVTPRDLDAAVLLLLLC+VRQH+P AL+TPIT
Sbjct: 71  RFLLSALRRLSRPFQTPSKSFPHHPSTAAVTPRDLDAAVLLLLLCDVRQHNPAALETPIT 130

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
           +WRSTLCRIYSDSLLT+SG+ATGGAG  IPYIETVARCWKFVGFV S +GGKARREVAAS
Sbjct: 131 QWRSTLCRIYSDSLLTISGIATGGAGAFIPYIETVARCWKFVGFVVSRNGGKARREVAAS 190

Query: 181 PVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240
           PVAVVELPSVAVGG  AVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC
Sbjct: 191 PVAVVELPSVAVGGCMAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 250

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTSDGD 271
           RFQLPTDDIFGEIQRLW+IL+KVGS+SDGD
Sbjct: 251 RFQLPTDDIFGEIQRLWDILVKVGSSSDGD 280

[Close]

BLAST of Spg033503 vs. NCBI nr
Match: KAG7027979.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 498.4 bits (1282), Expect = 4.0e-137
Identity = 245/270 (90.74%), Postives = 257/270 (95.19%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAALS LSPPHLSDLSHSIFSD+H  RRRLTFILSSPTLFS TLRHLNSLSLS+KSLLIA
Sbjct: 1   MAALSTLSPPHLSDLSHSIFSDVHTSRRRLTFILSSPTLFSFTLRHLNSLSLSNKSLLIA 60

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRLSRPFQ PSKS PHHPSTAAVTPRDLDAAVLLLLLC+VRQH+P AL+TPIT
Sbjct: 61  RFLLSALRRLSRPFQTPSKSFPHHPSTAAVTPRDLDAAVLLLLLCDVRQHNPAALETPIT 120

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
           +WRSTLCRIYSDSLLT+SG+ATGGAG  IPYIETVARCWKFVGFV S +GGKARREVAAS
Sbjct: 121 QWRSTLCRIYSDSLLTISGIATGGAGAFIPYIETVARCWKFVGFVVSRNGGKARREVAAS 180

Query: 181 PVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240
           PVAVVELPSVAVGG  AVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC
Sbjct: 181 PVAVVELPSVAVGGCMAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTSDGD 271
           RFQLPTDDIFGEIQRLW+IL+KVGS+SDGD
Sbjct: 241 RFQLPTDDIFGEIQRLWDILVKVGSSSDGD 270

[Close]

BLAST of Spg033503 vs. NCBI nr
Match: XP_023538929.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 498.0 bits (1281), Expect = 5.2e-137
Identity = 245/270 (90.74%), Postives = 256/270 (94.81%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAALS LSPPHLSDLSHSIFSD+H  RRRLTFILSSPTLFS TLRHLNSLSLS+KSLLIA
Sbjct: 11  MAALSTLSPPHLSDLSHSIFSDVHTSRRRLTFILSSPTLFSFTLRHLNSLSLSNKSLLIA 70

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRLSRPFQ PSKS PHHPSTAAVTPRDLDAAVLLLLLC+VRQH+P AL+TPIT
Sbjct: 71  RFLLSALRRLSRPFQTPSKSFPHHPSTAAVTPRDLDAAVLLLLLCDVRQHNPAALETPIT 130

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
           +WRSTLCRIYSDSLLT+SG+ATGGAG  IPYIETVARCWKFVGFV S +GGKARREVAAS
Sbjct: 131 RWRSTLCRIYSDSLLTISGIATGGAGAFIPYIETVARCWKFVGFVVSRNGGKARREVAAS 190

Query: 181 PVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240
           PVAVVELPSVAVGG  AVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC
Sbjct: 191 PVAVVELPSVAVGGCMAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 250

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTSDGD 271
           RFQLPTDDIFGEIQRLW+IL+KVGS SDGD
Sbjct: 251 RFQLPTDDIFGEIQRLWDILVKVGSPSDGD 280

[Close]

BLAST of Spg033503 vs. NCBI nr
Match: XP_022935892.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucurbita moschata])

HSP 1 Score: 498.0 bits (1281), Expect = 5.2e-137
Identity = 245/270 (90.74%), Postives = 256/270 (94.81%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAALS LS PHLSDLSHSIFSD+H  RRRLTFILSSPTLFS TLRHLNSLSLSHKSLLIA
Sbjct: 11  MAALSTLSSPHLSDLSHSIFSDVHTSRRRLTFILSSPTLFSFTLRHLNSLSLSHKSLLIA 70

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRLSRPFQ PSKS PHHPSTAAVTPRDLDAAVLLLLLC+VRQH+P AL+TPIT
Sbjct: 71  RFLLSALRRLSRPFQTPSKSFPHHPSTAAVTPRDLDAAVLLLLLCDVRQHNPAALETPIT 130

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
           +WRSTLCRIYSDSLLT+SG+ATGGAG  IPYIETVARCWKFVGFV S +GGKARREVAAS
Sbjct: 131 QWRSTLCRIYSDSLLTISGIATGGAGAFIPYIETVARCWKFVGFVVSRNGGKARREVAAS 190

Query: 181 PVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240
           PVAVVELPSVAVGG  AVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC
Sbjct: 191 PVAVVELPSVAVGGCMAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 250

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTSDGD 271
           RFQLPTDDIFGEIQRLW+IL+KVGS+SDGD
Sbjct: 251 RFQLPTDDIFGEIQRLWDILVKVGSSSDGD 280

[Close]

BLAST of Spg033503 vs. NCBI nr
Match: XP_023005737.1 (E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucurbita maxima])

HSP 1 Score: 495.0 bits (1273), Expect = 4.4e-136
Identity = 242/270 (89.63%), Postives = 254/270 (94.07%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAALS LSPPHLSDLSHSIFSD+H  RRRLTFILSSPTLFS TLRHLNSLSLSHKSLL+A
Sbjct: 11  MAALSTLSPPHLSDLSHSIFSDVHTSRRRLTFILSSPTLFSFTLRHLNSLSLSHKSLLVA 70

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRLSRPFQ PSKS PHHPSTA VTPRDLDAAVLLLLLC+VRQH+P AL+TPIT
Sbjct: 71  RFLLSALRRLSRPFQTPSKSFPHHPSTAFVTPRDLDAAVLLLLLCDVRQHNPAALETPIT 130

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
            WRSTLCRIYSDSLLT+SG+ATGG+G +IPYIETV RCWKFVGFV S +GGKARREVAAS
Sbjct: 131 HWRSTLCRIYSDSLLTISGIATGGSGAIIPYIETVGRCWKFVGFVVSRNGGKARREVAAS 190

Query: 181 PVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240
           PVAVVELPSVAVGG  AVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC
Sbjct: 191 PVAVVELPSVAVGGCMAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 250

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTSDGD 271
           RFQLPTDDIFGEIQRLW+IL+KVGS SDGD
Sbjct: 251 RFQLPTDDIFGEIQRLWDILVKVGSPSDGD 280

[Close]

BLAST of Spg033503 vs. ExPASy Swiss-Prot
Match: Q8GXF8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SGR9 PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.3e-69
Identity = 152/274 (55.47%), Postives = 181/274 (66.06%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MA+LSALSP HL++L+HSI S  HH RRRL  +LSSPTLFSLTLRHL SLSL  K+ LIA
Sbjct: 10  MASLSALSPSHLTNLTHSILSISHHHRRRLGAVLSSPTLFSLTLRHLLSLSLPDKTHLIA 69

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTP-RDLDAAVLLLLLCEVRQHSPTALQTPI 120
             LLS L        P      HH S A     RDLDA VLLL LCE  Q  P  L+   
Sbjct: 70  NHLLSLL-------HPLLIHRKHHSSYAVTMKLRDLDAVVLLLFLCETHQLHPDVLEASA 129

Query: 121 TKWRSTLCRIYSDSLLT-VSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKAR---- 180
             WR  L   YS+++L+  SG+ T  AG+L+PYIET+ RC +FV  +G  +  + R    
Sbjct: 130 DNWREILGNTYSNNMLSNNSGLWTCDAGILMPYIETLVRCKRFVDIMGGYNHLRRRDQKE 189

Query: 181 -REVAASPVAVVELPSVAVGGGAA-----VECVICKEEMNEGRDACQLPCEHLFHWLCIL 240
             +V A+  AVV L +V V   AA     VECVICKEEM+EGRD C++PC+H FHW CIL
Sbjct: 190 GYQVPAARAAVVALRAVEVFNVAASNAGEVECVICKEEMSEGRDVCEMPCQHFFHWKCIL 249

Query: 241 PWLRKRNTCPCCRFQLPTDDIFGEIQRLWEILLK 263
           PWL K+NTCP CRFQLPTDD+F EIQRLWEIL+K
Sbjct: 250 PWLSKKNTCPFCRFQLPTDDVFSEIQRLWEILVK 276

[Close]

BLAST of Spg033503 vs. ExPASy Swiss-Prot
Match: Q6GPV5 (E3 ubiquitin-protein ligase RNF181 OS=Xenopus laevis OX=8355 GN=rnf181 PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.4e-14
Identity = 39/72 (54.17%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 179 ASPVAVVELPSVAV---GGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRN 238
           AS   V  LP V V      AA++C +C  E  EG    QLPCEHLFH  CILPWL K N
Sbjct: 55  ASKKVVESLPKVTVTPEQADAALKCPVCLLEFEEGETVRQLPCEHLFHSACILPWLGKTN 114

Query: 239 TCPCCRFQLPTD 248
           +CP CR +LPTD
Sbjct: 115 SCPLCRHELPTD 126

[Close]

BLAST of Spg033503 vs. ExPASy Swiss-Prot
Match: Q5M974 (E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 9.8e-14
Identity = 38/72 (52.78%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 179 ASPVAVVELPSVAV---GGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRN 238
           A+   V  LP V V      AA++C +C  E  EG    QLPCEHLFH  CILPWL K N
Sbjct: 55  AAKKVVESLPKVTVTPEQADAALKCPVCLLEFEEGETVRQLPCEHLFHSSCILPWLGKTN 114

Query: 239 TCPCCRFQLPTD 248
           +CP CR +LPTD
Sbjct: 115 SCPLCRHELPTD 126

[Close]

BLAST of Spg033503 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 1.3e-13
Identity = 34/71 (47.89%), Postives = 44/71 (61.97%), Query Frame = 0


Query: 179 ASPVAVVELPSVAV-GGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTC 238
           A+   V  LP   + G  A ++C +C  E  E   A ++PC HLFH  CILPWL K N+C
Sbjct: 54  AAKTVVENLPRTVIRGSQAELKCPVCLLEFEEEETAIEMPCHHLFHSSCILPWLSKTNSC 113

Query: 239 PCCRFQLPTDD 249
           P CR++LPTDD
Sbjct: 114 PLCRYELPTDD 124

[Close]

BLAST of Spg033503 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.7e-13
Identity = 35/71 (49.30%), Postives = 44/71 (61.97%), Query Frame = 0


Query: 179 ASPVAVVELPSVAV-GGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTC 238
           A+  AV  LP   + G  A ++C +C  E  E   A ++PC HLFH  CILPWL K N+C
Sbjct: 54  AAKTAVENLPRTVIRGSQAELKCPVCLLEFEEEETAIEMPCHHLFHSNCILPWLSKTNSC 113

Query: 239 PCCRFQLPTDD 249
           P CR +LPTDD
Sbjct: 114 PLCRHELPTDD 124

[Close]

BLAST of Spg033503 vs. ExPASy TrEMBL
Match: A0A6J1F6V7 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucurbita moschata OX=3662 GN=LOC111442657 PE=4 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.5e-137
Identity = 245/270 (90.74%), Postives = 256/270 (94.81%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAALS LS PHLSDLSHSIFSD+H  RRRLTFILSSPTLFS TLRHLNSLSLSHKSLLIA
Sbjct: 11  MAALSTLSSPHLSDLSHSIFSDVHTSRRRLTFILSSPTLFSFTLRHLNSLSLSHKSLLIA 70

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRLSRPFQ PSKS PHHPSTAAVTPRDLDAAVLLLLLC+VRQH+P AL+TPIT
Sbjct: 71  RFLLSALRRLSRPFQTPSKSFPHHPSTAAVTPRDLDAAVLLLLLCDVRQHNPAALETPIT 130

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
           +WRSTLCRIYSDSLLT+SG+ATGGAG  IPYIETVARCWKFVGFV S +GGKARREVAAS
Sbjct: 131 QWRSTLCRIYSDSLLTISGIATGGAGAFIPYIETVARCWKFVGFVVSRNGGKARREVAAS 190

Query: 181 PVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240
           PVAVVELPSVAVGG  AVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC
Sbjct: 191 PVAVVELPSVAVGGCMAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 250

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTSDGD 271
           RFQLPTDDIFGEIQRLW+IL+KVGS+SDGD
Sbjct: 251 RFQLPTDDIFGEIQRLWDILVKVGSSSDGD 280

[Close]

BLAST of Spg033503 vs. ExPASy TrEMBL
Match: A0A6J1KTZ6 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucurbita maxima OX=3661 GN=LOC111498644 PE=4 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.1e-136
Identity = 242/270 (89.63%), Postives = 254/270 (94.07%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAALS LSPPHLSDLSHSIFSD+H  RRRLTFILSSPTLFS TLRHLNSLSLSHKSLL+A
Sbjct: 11  MAALSTLSPPHLSDLSHSIFSDVHTSRRRLTFILSSPTLFSFTLRHLNSLSLSHKSLLVA 70

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRLSRPFQ PSKS PHHPSTA VTPRDLDAAVLLLLLC+VRQH+P AL+TPIT
Sbjct: 71  RFLLSALRRLSRPFQTPSKSFPHHPSTAFVTPRDLDAAVLLLLLCDVRQHNPAALETPIT 130

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
            WRSTLCRIYSDSLLT+SG+ATGG+G +IPYIETV RCWKFVGFV S +GGKARREVAAS
Sbjct: 131 HWRSTLCRIYSDSLLTISGIATGGSGAIIPYIETVGRCWKFVGFVVSRNGGKARREVAAS 190

Query: 181 PVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240
           PVAVVELPSVAVGG  AVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC
Sbjct: 191 PVAVVELPSVAVGGCMAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 250

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGSTSDGD 271
           RFQLPTDDIFGEIQRLW+IL+KVGS SDGD
Sbjct: 251 RFQLPTDDIFGEIQRLWDILVKVGSPSDGD 280

[Close]

BLAST of Spg033503 vs. ExPASy TrEMBL
Match: A0A6J1CX45 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Momordica charantia OX=3673 GN=LOC111015097 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.0e-130
Identity = 235/273 (86.08%), Postives = 252/273 (92.31%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAALSALSP HLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA
Sbjct: 40  MAALSALSPLHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 99

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRL  PFQPP+KSLP HPSTAA+TPRDLDAAVLLLLLCEVRQH+P AL+TP+ 
Sbjct: 100 RFLLSALRRLCHPFQPPAKSLPQHPSTAAITPRDLDAAVLLLLLCEVRQHNPAALETPLP 159

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
           KWR+ LCRIYSDS+L++SG+ +GG  V++PY+ETVARCWKFVGFVGSC GGK +REVAAS
Sbjct: 160 KWRAALCRIYSDSILSISGIISGGGAVMVPYVETVARCWKFVGFVGSC-GGKGKREVAAS 219

Query: 181 PVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240
           PVAVVELPSVA    AAVECVICKEEM EGRDACQLPCEHLFHWLCILPWLRKRNTCPCC
Sbjct: 220 PVAVVELPSVA--AAAAVECVICKEEMGEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 279

Query: 241 RFQLPTDDIFGEIQRLWEILLKVGS---TSDGD 271
           RFQLPTDD+F EIQRLWEILLK+GS   TSDGD
Sbjct: 280 RFQLPTDDVFAEIQRLWEILLKLGSTITTSDGD 309

[Close]

BLAST of Spg033503 vs. ExPASy TrEMBL
Match: A0A5A7TLA8 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002040 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.3e-130
Identity = 234/275 (85.09%), Postives = 255/275 (92.73%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAAL+ LSPP L+DLSHSIFS++H  RRRLTFILSSPTLFSLTLRHLNSLSLSHKSLL+A
Sbjct: 1   MAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 60

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRLSRPFQ PSK LP+HPSTAA++P+DLDAAVLLLLLCEVRQH+P AL+TPIT
Sbjct: 61  RFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 120

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
           KWR+TLCRIYS+SLLT+SG+ATGG G LIPYI+TV RCWKFVGFVGSC GGK RREVAAS
Sbjct: 121 KWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSC-GGKVRREVAAS 180

Query: 181 PVAVVELPSVAV-GGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPC 240
           P+AVVELPSVAV GGG  VECVICKEEM EGRDAC+LPC+HLFHWLCILPWLRKRNTCPC
Sbjct: 181 PMAVVELPSVAVGGGGGTVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPC 240

Query: 241 CRFQLPTDDIFGEIQRLWEILLKVGS---TSDGDQ 272
           CRFQLPTDD+FGEIQRLWEIL KVGS   TSDGD+
Sbjct: 241 CRFQLPTDDVFGEIQRLWEILFKVGSTMCTSDGDE 274

[Close]

BLAST of Spg033503 vs. ExPASy TrEMBL
Match: A0A1S4DUS9 (E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo OX=3656 GN=LOC103486430 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 1.3e-130
Identity = 234/275 (85.09%), Postives = 255/275 (92.73%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MAAL+ LSPP L+DLSHSIFS++H  RRRLTFILSSPTLFSLTLRHLNSLSLSHKSLL+A
Sbjct: 11  MAALATLSPPRLTDLSHSIFSEVHQHRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLLA 70

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTPRDLDAAVLLLLLCEVRQHSPTALQTPIT 120
           RFLLSALRRLSRPFQ PSK LP+HPSTAA++P+DLDAAVLLLLLCEVRQH+P AL+TPIT
Sbjct: 71  RFLLSALRRLSRPFQSPSKLLPYHPSTAAISPQDLDAAVLLLLLCEVRQHNPAALRTPIT 130

Query: 121 KWRSTLCRIYSDSLLTVSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKARREVAAS 180
           KWR+TLCRIYS+SLLT+SG+ATGG G LIPYI+TV RCWKFVGFVGSC GGK RREVAAS
Sbjct: 131 KWRATLCRIYSESLLTISGLATGGGGALIPYIDTVVRCWKFVGFVGSC-GGKVRREVAAS 190

Query: 181 PVAVVELPSVAV-GGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPC 240
           P+AVVELPSVAV GGG  VECVICKEEM EGRDAC+LPC+HLFHWLCILPWLRKRNTCPC
Sbjct: 191 PMAVVELPSVAVGGGGGTVECVICKEEMGEGRDACKLPCDHLFHWLCILPWLRKRNTCPC 250

Query: 241 CRFQLPTDDIFGEIQRLWEILLKVGS---TSDGDQ 272
           CRFQLPTDD+FGEIQRLWEIL KVGS   TSDGD+
Sbjct: 251 CRFQLPTDDVFGEIQRLWEILFKVGSTMCTSDGDE 284

[Close]

BLAST of Spg033503 vs. TAIR 10
Match: AT5G02750.1 (RING/U-box superfamily protein )

HSP 1 Score: 264.6 bits (675), Expect = 9.0e-71
Identity = 152/274 (55.47%), Postives = 181/274 (66.06%), Query Frame = 0


Query: 1   MAALSALSPPHLSDLSHSIFSDIHHRRRRLTFILSSPTLFSLTLRHLNSLSLSHKSLLIA 60
           MA+LSALSP HL++L+HSI S  HH RRRL  +LSSPTLFSLTLRHL SLSL  K+ LIA
Sbjct: 10  MASLSALSPSHLTNLTHSILSISHHHRRRLGAVLSSPTLFSLTLRHLLSLSLPDKTHLIA 69

Query: 61  RFLLSALRRLSRPFQPPSKSLPHHPSTAAVTP-RDLDAAVLLLLLCEVRQHSPTALQTPI 120
             LLS L        P      HH S A     RDLDA VLLL LCE  Q  P  L+   
Sbjct: 70  NHLLSLL-------HPLLIHRKHHSSYAVTMKLRDLDAVVLLLFLCETHQLHPDVLEASA 129

Query: 121 TKWRSTLCRIYSDSLLT-VSGVATGGAGVLIPYIETVARCWKFVGFVGSCSGGKAR---- 180
             WR  L   YS+++L+  SG+ T  AG+L+PYIET+ RC +FV  +G  +  + R    
Sbjct: 130 DNWREILGNTYSNNMLSNNSGLWTCDAGILMPYIETLVRCKRFVDIMGGYNHLRRRDQKE 189

Query: 181 -REVAASPVAVVELPSVAVGGGAA-----VECVICKEEMNEGRDACQLPCEHLFHWLCIL 240
             +V A+  AVV L +V V   AA     VECVICKEEM+EGRD C++PC+H FHW CIL
Sbjct: 190 GYQVPAARAAVVALRAVEVFNVAASNAGEVECVICKEEMSEGRDVCEMPCQHFFHWKCIL 249

Query: 241 PWLRKRNTCPCCRFQLPTDDIFGEIQRLWEILLK 263
           PWL K+NTCP CRFQLPTDD+F EIQRLWEIL+K
Sbjct: 250 PWLSKKNTCPFCRFQLPTDDVFSEIQRLWEILVK 276

[Close]

BLAST of Spg033503 vs. TAIR 10
Match: AT5G08139.1 (RING/U-box superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 4.8e-16
Identity = 36/68 (52.94%), Postives = 47/68 (69.12%), Query Frame = 0


Query: 181 PVAVVELPSVAVGGGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCC 240
           PV ++E  +   GG   + C +CK+EMN G  A QLPC H +H  CI+PWL+ RNTCP C
Sbjct: 293 PVVLLEGENDDDGG---LVCAVCKDEMNIGNKAVQLPCNHKYHSECIVPWLKVRNTCPVC 352

Query: 241 RFQLPTDD 249
           R++LPTDD
Sbjct: 353 RYELPTDD 357

[Close]

BLAST of Spg033503 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.6e-14
Identity = 34/74 (45.95%), Postives = 46/74 (62.16%), Query Frame = 0


Query: 179 ASPVAVVELPSVAVG----GGAAVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKR 238
           AS  A+  LP+V V          +C +C +E  +G D  Q+PC+H+FH  C+LPWL   
Sbjct: 191 ASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELH 250

Query: 239 NTCPCCRFQLPTDD 249
           N+CP CRF+LPTDD
Sbjct: 251 NSCPVCRFELPTDD 264

[Close]

BLAST of Spg033503 vs. TAIR 10
Match: AT5G60820.1 (RING/U-box superfamily protein )

HSP 1 Score: 77.4 bits (189), Expect = 2.0e-14
Identity = 30/51 (58.82%), Postives = 38/51 (74.51%), Query Frame = 0


Query: 197 AVECVICKEEMNEGRDACQLPCEHLFHWLCILPWLRKRNTCPCCRFQLPTD 248
           AV C +CKEEM  G++  +LPC H +H  CI+PWL  RNTCP CRF+LP+D
Sbjct: 369 AVCCAVCKEEMIVGKEVAELPCRHKYHSECIVPWLGIRNTCPVCRFELPSD 419

[Close]

BLAST of Spg033503 vs. TAIR 10
Match: AT5G64920.1 (COP1-interacting protein 8 )

HSP 1 Score: 73.6 bits (179), Expect = 2.9e-13
Identity = 40/94 (42.55%), Postives = 50/94 (53.19%), Query Frame = 0


Query: 166 GSCSGGKARR---EVAASPVAVVELPSVAVGGGAAV-ECVICKEEMNEGRDACQLPCEHL 225
           G   GG  RR     A S +  +E   V+   G  V  C +CK+ M  G    +LPC H 
Sbjct: 219 GDGGGGGGRRGAPPAAKSAIEALETFEVSSSEGEMVMVCAVCKDGMVMGETGKKLPCGHC 278

Query: 226 FHWLCILPWLRKRNTCPCCRFQLPTDDIFGEIQR 256
           +H  CI+PWL  RN+CP CRFQL TDD   E +R
Sbjct: 279 YHGDCIVPWLGTRNSCPVCRFQLETDDAEYEEER 312

The following BLAST results are available for this feature:

BLAST of Spg033503 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596438.1	6.1e-138	91.11	E3 ubiquitin-protein ligase SGR9, amyloplastic, partial [Cucurbita argyrosperma ...	[more]
KAG7027979.1	4.0e-137	90.74	E3 ubiquitin-protein ligase SGR9, amyloplastic, partial [Cucurbita argyrosperma ...	[more]
XP_023538929.1	5.2e-137	90.74	E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022935892.1	5.2e-137	90.74	E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucurbita moschata]	[more]
XP_023005737.1	4.4e-136	89.63	E3 ubiquitin-protein ligase SGR9, amyloplastic [Cucurbita maxima]	[more]

BLAST of Spg033503 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GXF8	1.3e-69	55.47	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
Q6GPV5	3.4e-14	54.17	E3 ubiquitin-protein ligase RNF181 OS=Xenopus laevis OX=8355 GN=rnf181 PE=2 SV=1	[more]
Q5M974	9.8e-14	52.78	E3 ubiquitin-protein ligase RNF181 OS=Xenopus tropicalis OX=8364 GN=rnf181 PE=2 ...	[more]
Q9P0P0	1.3e-13	47.89	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]
Q3T0W3	1.7e-13	49.30	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]

BLAST of Spg033503 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F6V7	2.5e-137	90.74	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1KTZ6	2.1e-136	89.63	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1CX45	1.0e-130	86.08	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Momordica charantia OX=3673 GN...	[more]
A0A5A7TLA8	1.3e-130	85.09	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S4DUS9	1.3e-130	85.09	E3 ubiquitin-protein ligase SGR9, amyloplastic OS=Cucumis melo OX=3656 GN=LOC103...	[more]

BLAST of Spg033503 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02750.1	9.0e-71	55.47	RING/U-box superfamily protein	[more]
AT5G08139.1	4.8e-16	52.94	RING/U-box superfamily protein	[more]
AT3G19950.1	1.6e-14	45.95	RING/U-box superfamily protein	[more]
AT5G60820.1	2.0e-14	58.82	RING/U-box superfamily protein	[more]
AT5G64920.1	2.9e-13	42.55	COP1-interacting protein 8	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 200..240 e-value: 7.0E-6 score: 35.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 199..241 e-value: 6.4E-11 score: 42.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 200..241 score: 12.1057
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 193..251 e-value: 1.2E-17 score: 64.9
None	No IPR available	PANTHER	PTHR15710:SF67	E3 UBIQUITIN-PROTEIN LIGASE SGR9, AMYLOPLASTIC	coord: 1..266
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 1..266
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 194..247

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg033503.1	Spg033503.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009630	gravitropism
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0009501	amyloplast
molecular_function	GO:0061630	ubiquitin protein ligase activity