Spg033500 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg033500 vs. NCBI nr
Match: KAG6596456.1 (hypothetical protein SDJN03_09636, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1024.2 bits (2647), Expect = 4.3e-295
Identity = 496/558 (88.89%), Postives = 521/558 (93.37%), Query Frame = 0

Query: 28  ERRVNHRWCEPGGAGAVEVFWARGDVDKKSDIIVLGSSSWSEELASLVEDSGIRYVAEPI 87
           +RR N RW E   +  VE    +  + +     + GSSSWSEE ASLVEDSGIRY+AEPI
Sbjct: 45  KRRANRRWTEFDVSEVVEGVSIQLPMSE-----IRGSSSWSEEFASLVEDSGIRYMAEPI 104

Query: 88  GVSSPSFENTRSDFQVESRNYEPEESESLKDQVKGFAVAWGEILLELGRGCRDIVQQNLI 147
           G+SSPS+E+TRSDFQVESRNYE EESESLKDQVKGFAVAWGEILLELGRGCRDIVQQNLI
Sbjct: 105 GISSPSYESTRSDFQVESRNYESEESESLKDQVKGFAVAWGEILLELGRGCRDIVQQNLI 164

Query: 148 TEDSYVGQKLRGPCANVTARLSFLNEFLPEDRDPVYAWPVIFFVSILAFTVICVNNRHES 207
           TEDSYV QKLRGPCANV ARLSFLNEFLPEDRDPVYAWPVIFFVSILAFTVICVNNRHES
Sbjct: 165 TEDSYVAQKLRGPCANVLARLSFLNEFLPEDRDPVYAWPVIFFVSILAFTVICVNNRHES 224

Query: 208 FSRPIMKVRDHPPCATRILLPDGRHMAYDVLGVSADRARFSIIAPHSFLSSRFAGIPGVK 267
           FSRPIMKVRDHPP A+RILLPDGRH+AYDVLGVSADRARF IIAPHSFLSSRFAGIPGVK
Sbjct: 225 FSRPIMKVRDHPPSASRILLPDGRHLAYDVLGVSADRARFFIIAPHSFLSSRFAGIPGVK 284

Query: 268 TSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSSAFDMLHLADAVSITGKFWVLGYSEGAM 327
           +SLLEEFG+ LVAYDLPGFGESDPHPNRNLNSSA DMLHLADAVSI GKFWVLGYSEGAM
Sbjct: 285 SSLLEEFGICLVAYDLPGFGESDPHPNRNLNSSALDMLHLADAVSINGKFWVLGYSEGAM 344

Query: 328 HVWAALRYIPDRVAGAIMVAPMINPYEKTLTREELRRTWENWGPRKRLLYFLARRFPRFL 387
           H WAALRYIPDR+AG +MVAPMINPYEK++TREELRRTWE+WGPRKR LYFLARRFPRFL
Sbjct: 345 HAWAALRYIPDRIAGTVMVAPMINPYEKSMTREELRRTWESWGPRKRFLYFLARRFPRFL 404

Query: 388 SYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIEDPKFREFWYRNVEESIRQKNVKPFIEE 447
           SYFYRRSFLSG+HEEIDRQLSLSLGKKDEVLIEDPKFREFWYRNVEESIRQKNVKPF+EE
Sbjct: 405 SYFYRRSFLSGRHEEIDRQLSLSLGKKDEVLIEDPKFREFWYRNVEESIRQKNVKPFVEE 464

Query: 448 TMLLVSNWGFSLADLRVQRKCQRTGILTWLKSLYSQEECELAGFVGPIHIWQGIDDKVVP 507
           TMLLVSNWGFSLADLRVQRKCQRTGIL WLKSLYSQEECELAGFVGPIHIWQGIDDKVVP
Sbjct: 465 TMLLVSNWGFSLADLRVQRKCQRTGILPWLKSLYSQEECELAGFVGPIHIWQGIDDKVVP 524

Query: 508 PSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDECHRQIFSTIFGPPKGPVDRRERMEASP 567
           PSMTDYIGRILPAA+LHKLSNEGHFS+LYFCDECHRQIFSTIFGPPKGPVDRRER EASP
Sbjct: 525 PSMTDYIGRILPAAILHKLSNEGHFSYLYFCDECHRQIFSTIFGPPKGPVDRRERTEASP 584

Query: 568 LEGNI-GEVATTIDLTVK 585
           LEGN  GE+A+TIDLTVK
Sbjct: 585 LEGNTGGEIASTIDLTVK 597

BLAST of Spg033500 vs. NCBI nr
Match: XP_022945536.1 (uncharacterized protein LOC111449740 [Cucurbita moschata] >XP_022945546.1 uncharacterized protein LOC111449740 [Cucurbita moschata] >XP_022945552.1 uncharacterized protein LOC111449740 [Cucurbita moschata])

HSP 1 Score: 1016.9 bits (2628), Expect = 6.9e-293
Identity = 488/525 (92.95%), Postives = 508/525 (96.76%), Query Frame = 0

Query: 61  VLGSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNYEPEESESLKDQV 120
           + GSSSWSEE ASLVEDSGIRY+AEPIG+SSPS+E+TRSDFQVESRNYE EESESLKDQV
Sbjct: 4   IRGSSSWSEEFASLVEDSGIRYMAEPIGISSPSYESTRSDFQVESRNYESEESESLKDQV 63

Query: 121 KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRD 180
           KGFAVAWGEILLELGRGCRDIVQQNLITEDSYV QKLRGPCANV ARLSFLNEFLPEDRD
Sbjct: 64  KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVAQKLRGPCANVLARLSFLNEFLPEDRD 123

Query: 181 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGV 240
           PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPP A+RILLPDGRH+AYDVLGV
Sbjct: 124 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPSASRILLPDGRHLAYDVLGV 183

Query: 241 SADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSS 300
           SADRARF IIAPHSFLSSRFAGIPGVK+SLLEEFG+ LVAYDLPGFGESDPHPNRNLNSS
Sbjct: 184 SADRARFFIIAPHSFLSSRFAGIPGVKSSLLEEFGICLVAYDLPGFGESDPHPNRNLNSS 243

Query: 301 AFDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTRE 360
           A DMLHLADAVSI GKFWVLGYSEGAMH WAALRYIPDR+AGA+MVAPMINPYEK++TRE
Sbjct: 244 ALDMLHLADAVSINGKFWVLGYSEGAMHAWAALRYIPDRIAGAVMVAPMINPYEKSMTRE 303

Query: 361 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 420
           ELRRTWE+WGPRKR LYFLARRFPRFLSYFYRRSFLSG+HEEIDRQLSLSLGKKDEVLIE
Sbjct: 304 ELRRTWESWGPRKRFLYFLARRFPRFLSYFYRRSFLSGRHEEIDRQLSLSLGKKDEVLIE 363

Query: 421 DPKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSL 480
           DPKFREFWYRNVEESIRQKNVKPF+EETMLLVSNWGFSLADLRVQRKCQRTGIL WLKSL
Sbjct: 364 DPKFREFWYRNVEESIRQKNVKPFVEETMLLVSNWGFSLADLRVQRKCQRTGILPWLKSL 423

Query: 481 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDE 540
           YSQEECELAGFVGPIHIWQGIDDKVVP SMTDYIGRILPAA+LHKLSNEGHFS+LYFCDE
Sbjct: 424 YSQEECELAGFVGPIHIWQGIDDKVVPRSMTDYIGRILPAAILHKLSNEGHFSYLYFCDE 483

Query: 541 CHRQIFSTIFGPPKGPVDRRERMEASPLEGNI-GEVATTIDLTVK 585
           CHRQIFSTIFGPPKGPVDRRER EASPLEGN  GE+A+TIDLTVK
Sbjct: 484 CHRQIFSTIFGPPKGPVDRRERTEASPLEGNTGGEIASTIDLTVK 528

BLAST of Spg033500 vs. NCBI nr
Match: XP_023540184.1 (uncharacterized protein LOC111800631 [Cucurbita pepo subsp. pepo] >XP_023540186.1 uncharacterized protein LOC111800631 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1013.8 bits (2620), Expect = 5.8e-292
Identity = 485/525 (92.38%), Postives = 507/525 (96.57%), Query Frame = 0

Query: 61  VLGSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNYEPEESESLKDQV 120
           + GSSSWSEE ASLVEDSGIRY+AEPIG+SSPS+E+TRSDFQVESRNYE EESESLKDQV
Sbjct: 4   IRGSSSWSEEFASLVEDSGIRYMAEPIGISSPSYESTRSDFQVESRNYESEESESLKDQV 63

Query: 121 KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRD 180
           KGFAVAWGEILLELGRGCRDIVQQNLITEDSYV QKLRGPC NV ARLSFLNEFLPEDRD
Sbjct: 64  KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVAQKLRGPCENVIARLSFLNEFLPEDRD 123

Query: 181 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGV 240
           PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPP A+RILLPDGRH+AYDVLGV
Sbjct: 124 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPSASRILLPDGRHLAYDVLGV 183

Query: 241 SADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSS 300
           SADRARF IIAPHSFLSSRFAGIPGVK+SLLEEFG+ LVAYDLPGFGESDPHPNRNLNSS
Sbjct: 184 SADRARFFIIAPHSFLSSRFAGIPGVKSSLLEEFGICLVAYDLPGFGESDPHPNRNLNSS 243

Query: 301 AFDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTRE 360
           A DMLHLADAVSI GKFWVLGYSEGAMH WAALRYIPDR+AGA+MVAPMINPYEK++TRE
Sbjct: 244 ALDMLHLADAVSINGKFWVLGYSEGAMHAWAALRYIPDRIAGAVMVAPMINPYEKSMTRE 303

Query: 361 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 420
           ELRRTWE+WGPRKR LYFLAR FPRFLSYFYRRSFLSG+HEEIDRQLSLSLGKKDEVLIE
Sbjct: 304 ELRRTWESWGPRKRFLYFLARSFPRFLSYFYRRSFLSGRHEEIDRQLSLSLGKKDEVLIE 363

Query: 421 DPKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSL 480
           D KFREFWYRNVEESIRQKNVKPF+EETMLLVSNWGFSLADLRVQRKCQRTGIL WLKSL
Sbjct: 364 DSKFREFWYRNVEESIRQKNVKPFVEETMLLVSNWGFSLADLRVQRKCQRTGILPWLKSL 423

Query: 481 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDE 540
           YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAA+LHKLSNEGHFS+LYFCDE
Sbjct: 424 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAILHKLSNEGHFSYLYFCDE 483

Query: 541 CHRQIFSTIFGPPKGPVDRRERMEASPLEGNI-GEVATTIDLTVK 585
           CHRQIFSTIFGPPKGPVDRRE+MEASPLEGN  GE+A+T+DLTVK
Sbjct: 484 CHRQIFSTIFGPPKGPVDRREKMEASPLEGNTGGEIASTVDLTVK 528

BLAST of Spg033500 vs. NCBI nr
Match: XP_023005769.1 (uncharacterized protein LOC111498670 [Cucurbita maxima] >XP_023005770.1 uncharacterized protein LOC111498670 [Cucurbita maxima] >XP_023005771.1 uncharacterized protein LOC111498670 [Cucurbita maxima])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.6e-292
Identity = 487/525 (92.76%), Postives = 506/525 (96.38%), Query Frame = 0

Query: 61  VLGSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNYEPEESESLKDQV 120
           + GSSSWSEE  SLVEDSGIRY+AEPIG+SSPS+E+TRSDFQVESRNYE EESESLKDQV
Sbjct: 4   IRGSSSWSEEFGSLVEDSGIRYMAEPIGISSPSYESTRSDFQVESRNYESEESESLKDQV 63

Query: 121 KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRD 180
           KGFAVAWGEILLELGRGCRDIVQQNLITEDSYV QKLRGPCANV ARLSFLNEFLPEDRD
Sbjct: 64  KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVAQKLRGPCANVIARLSFLNEFLPEDRD 123

Query: 181 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGV 240
           PVYAWPVIFFVSILAFTVI VNNRHESFSRPIMKVRDHPP A+R LLPDGRH+AYDVLGV
Sbjct: 124 PVYAWPVIFFVSILAFTVIWVNNRHESFSRPIMKVRDHPPSASRTLLPDGRHLAYDVLGV 183

Query: 241 SADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSS 300
           SADRARF IIAPHSFLSSRFAGIPGVK+SLLEEFGV LVAYDLPGFGESDPHPNRNLNSS
Sbjct: 184 SADRARFFIIAPHSFLSSRFAGIPGVKSSLLEEFGVCLVAYDLPGFGESDPHPNRNLNSS 243

Query: 301 AFDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTRE 360
           A DMLHLADAVSI GKFWVLGYSEGAMH WAALRYIPDR+AGA+MVAPMINPYEK++TRE
Sbjct: 244 ALDMLHLADAVSINGKFWVLGYSEGAMHAWAALRYIPDRIAGAVMVAPMINPYEKSMTRE 303

Query: 361 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 420
           ELRRTWE+WGPRKR LYFLARRFPRFLSYFYRRSFLSG+HEEIDRQLSLSLGKKDEVLIE
Sbjct: 304 ELRRTWESWGPRKRFLYFLARRFPRFLSYFYRRSFLSGRHEEIDRQLSLSLGKKDEVLIE 363

Query: 421 DPKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSL 480
           DPKFREFWYRNVEESIRQKNVKPF+EETMLLVSNWGFSLADLRVQRKCQRTGIL WLKSL
Sbjct: 364 DPKFREFWYRNVEESIRQKNVKPFVEETMLLVSNWGFSLADLRVQRKCQRTGILPWLKSL 423

Query: 481 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDE 540
           YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAA+LHKLSNEGHFS+LYFCDE
Sbjct: 424 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAILHKLSNEGHFSYLYFCDE 483

Query: 541 CHRQIFSTIFGPPKGPVDRRERMEASPLEGNI-GEVATTIDLTVK 585
           CHRQIFSTIFGPPKGPVDRRERMEASPLEGN  GE+ +TIDLTVK
Sbjct: 484 CHRQIFSTIFGPPKGPVDRRERMEASPLEGNTGGEIESTIDLTVK 528

BLAST of Spg033500 vs. NCBI nr
Match: XP_022145679.1 (uncharacterized protein LOC111015071 [Momordica charantia])

HSP 1 Score: 1010.7 bits (2612), Expect = 4.9e-291
Identity = 489/524 (93.32%), Postives = 502/524 (95.80%), Query Frame = 0

Query: 61  VLGSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNYEPEESESLKDQV 120
           V GSSSWSEELASLVEDSGIRYVAEPIG+SSPSF+NTR DFQVESRNYE EESESLKDQV
Sbjct: 4   VRGSSSWSEELASLVEDSGIRYVAEPIGISSPSFDNTRLDFQVESRNYESEESESLKDQV 63

Query: 121 KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRD 180
           KGFAVAWGEILLELGRGCRDIVQQNLITEDSYV +KLRGPCANVTARLSFLNEFLPEDRD
Sbjct: 64  KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVARKLRGPCANVTARLSFLNEFLPEDRD 123

Query: 181 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGV 240
           PVYAWPVIFFVSILAFTVI VNNRHESFSRPIMKVRDHPP ATRILLPDGRHMAYDVLGV
Sbjct: 124 PVYAWPVIFFVSILAFTVIFVNNRHESFSRPIMKVRDHPPSATRILLPDGRHMAYDVLGV 183

Query: 241 SADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSS 300
           SADRARFSIIAPHSFLSSR AGIPGVKTSLLEEFGVRLV+YDLPGFGESDPHPNRNLNSS
Sbjct: 184 SADRARFSIIAPHSFLSSRLAGIPGVKTSLLEEFGVRLVSYDLPGFGESDPHPNRNLNSS 243

Query: 301 AFDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTRE 360
           AFDMLHLADA+SI+GKFWVLG+SEGAMH WAALRYIPD  AG IMVAPMINPYEK +TRE
Sbjct: 244 AFDMLHLADAISISGKFWVLGHSEGAMHAWAALRYIPDEFAGTIMVAPMINPYEKIMTRE 303

Query: 361 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 420
           ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE
Sbjct: 304 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 363

Query: 421 DPKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSL 480
           DPKFREFWYRNVEESIRQKNVKPFIEET+L VSNWGFSLADLRVQRKCQR GIL WLKSL
Sbjct: 364 DPKFREFWYRNVEESIRQKNVKPFIEETVLQVSNWGFSLADLRVQRKCQRAGILPWLKSL 423

Query: 481 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDE 540
           YSQEECELAGFVGPIHIWQGIDD+VVPPSMTDYIGRILPAA+LHKLSNEGHFSFLYFCDE
Sbjct: 424 YSQEECELAGFVGPIHIWQGIDDQVVPPSMTDYIGRILPAAILHKLSNEGHFSFLYFCDE 483

Query: 541 CHRQIFSTIFGPPKGPVDRRERMEASPLEGNIGEVATTIDLTVK 585
           CHRQIFSTIFG PKGPVDRRERMEASP EGN  +VATT D  V+
Sbjct: 484 CHRQIFSTIFGIPKGPVDRRERMEASPFEGNTRDVATTTDPIVE 527

BLAST of Spg033500 vs. ExPASy TrEMBL
Match: A0A6J1G155 (uncharacterized protein LOC111449740 OS=Cucurbita moschata OX=3662 GN=LOC111449740 PE=4 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 3.3e-293
Identity = 488/525 (92.95%), Postives = 508/525 (96.76%), Query Frame = 0

Query: 61  VLGSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNYEPEESESLKDQV 120
           + GSSSWSEE ASLVEDSGIRY+AEPIG+SSPS+E+TRSDFQVESRNYE EESESLKDQV
Sbjct: 4   IRGSSSWSEEFASLVEDSGIRYMAEPIGISSPSYESTRSDFQVESRNYESEESESLKDQV 63

Query: 121 KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRD 180
           KGFAVAWGEILLELGRGCRDIVQQNLITEDSYV QKLRGPCANV ARLSFLNEFLPEDRD
Sbjct: 64  KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVAQKLRGPCANVLARLSFLNEFLPEDRD 123

Query: 181 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGV 240
           PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPP A+RILLPDGRH+AYDVLGV
Sbjct: 124 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPSASRILLPDGRHLAYDVLGV 183

Query: 241 SADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSS 300
           SADRARF IIAPHSFLSSRFAGIPGVK+SLLEEFG+ LVAYDLPGFGESDPHPNRNLNSS
Sbjct: 184 SADRARFFIIAPHSFLSSRFAGIPGVKSSLLEEFGICLVAYDLPGFGESDPHPNRNLNSS 243

Query: 301 AFDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTRE 360
           A DMLHLADAVSI GKFWVLGYSEGAMH WAALRYIPDR+AGA+MVAPMINPYEK++TRE
Sbjct: 244 ALDMLHLADAVSINGKFWVLGYSEGAMHAWAALRYIPDRIAGAVMVAPMINPYEKSMTRE 303

Query: 361 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 420
           ELRRTWE+WGPRKR LYFLARRFPRFLSYFYRRSFLSG+HEEIDRQLSLSLGKKDEVLIE
Sbjct: 304 ELRRTWESWGPRKRFLYFLARRFPRFLSYFYRRSFLSGRHEEIDRQLSLSLGKKDEVLIE 363

Query: 421 DPKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSL 480
           DPKFREFWYRNVEESIRQKNVKPF+EETMLLVSNWGFSLADLRVQRKCQRTGIL WLKSL
Sbjct: 364 DPKFREFWYRNVEESIRQKNVKPFVEETMLLVSNWGFSLADLRVQRKCQRTGILPWLKSL 423

Query: 481 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDE 540
           YSQEECELAGFVGPIHIWQGIDDKVVP SMTDYIGRILPAA+LHKLSNEGHFS+LYFCDE
Sbjct: 424 YSQEECELAGFVGPIHIWQGIDDKVVPRSMTDYIGRILPAAILHKLSNEGHFSYLYFCDE 483

Query: 541 CHRQIFSTIFGPPKGPVDRRERMEASPLEGNI-GEVATTIDLTVK 585
           CHRQIFSTIFGPPKGPVDRRER EASPLEGN  GE+A+TIDLTVK
Sbjct: 484 CHRQIFSTIFGPPKGPVDRRERTEASPLEGNTGGEIASTIDLTVK 528

BLAST of Spg033500 vs. ExPASy TrEMBL
Match: A0A6J1KYB4 (uncharacterized protein LOC111498670 OS=Cucurbita maxima OX=3661 GN=LOC111498670 PE=4 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.7e-292
Identity = 487/525 (92.76%), Postives = 506/525 (96.38%), Query Frame = 0

Query: 61  VLGSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNYEPEESESLKDQV 120
           + GSSSWSEE  SLVEDSGIRY+AEPIG+SSPS+E+TRSDFQVESRNYE EESESLKDQV
Sbjct: 4   IRGSSSWSEEFGSLVEDSGIRYMAEPIGISSPSYESTRSDFQVESRNYESEESESLKDQV 63

Query: 121 KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRD 180
           KGFAVAWGEILLELGRGCRDIVQQNLITEDSYV QKLRGPCANV ARLSFLNEFLPEDRD
Sbjct: 64  KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVAQKLRGPCANVIARLSFLNEFLPEDRD 123

Query: 181 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGV 240
           PVYAWPVIFFVSILAFTVI VNNRHESFSRPIMKVRDHPP A+R LLPDGRH+AYDVLGV
Sbjct: 124 PVYAWPVIFFVSILAFTVIWVNNRHESFSRPIMKVRDHPPSASRTLLPDGRHLAYDVLGV 183

Query: 241 SADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSS 300
           SADRARF IIAPHSFLSSRFAGIPGVK+SLLEEFGV LVAYDLPGFGESDPHPNRNLNSS
Sbjct: 184 SADRARFFIIAPHSFLSSRFAGIPGVKSSLLEEFGVCLVAYDLPGFGESDPHPNRNLNSS 243

Query: 301 AFDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTRE 360
           A DMLHLADAVSI GKFWVLGYSEGAMH WAALRYIPDR+AGA+MVAPMINPYEK++TRE
Sbjct: 244 ALDMLHLADAVSINGKFWVLGYSEGAMHAWAALRYIPDRIAGAVMVAPMINPYEKSMTRE 303

Query: 361 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 420
           ELRRTWE+WGPRKR LYFLARRFPRFLSYFYRRSFLSG+HEEIDRQLSLSLGKKDEVLIE
Sbjct: 304 ELRRTWESWGPRKRFLYFLARRFPRFLSYFYRRSFLSGRHEEIDRQLSLSLGKKDEVLIE 363

Query: 421 DPKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSL 480
           DPKFREFWYRNVEESIRQKNVKPF+EETMLLVSNWGFSLADLRVQRKCQRTGIL WLKSL
Sbjct: 364 DPKFREFWYRNVEESIRQKNVKPFVEETMLLVSNWGFSLADLRVQRKCQRTGILPWLKSL 423

Query: 481 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDE 540
           YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAA+LHKLSNEGHFS+LYFCDE
Sbjct: 424 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAILHKLSNEGHFSYLYFCDE 483

Query: 541 CHRQIFSTIFGPPKGPVDRRERMEASPLEGNI-GEVATTIDLTVK 585
           CHRQIFSTIFGPPKGPVDRRERMEASPLEGN  GE+ +TIDLTVK
Sbjct: 484 CHRQIFSTIFGPPKGPVDRRERMEASPLEGNTGGEIESTIDLTVK 528

BLAST of Spg033500 vs. ExPASy TrEMBL
Match: A0A6J1CVY7 (uncharacterized protein LOC111015071 OS=Momordica charantia OX=3673 GN=LOC111015071 PE=4 SV=1)

HSP 1 Score: 1010.7 bits (2612), Expect = 2.4e-291
Identity = 489/524 (93.32%), Postives = 502/524 (95.80%), Query Frame = 0

Query: 61  VLGSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNYEPEESESLKDQV 120
           V GSSSWSEELASLVEDSGIRYVAEPIG+SSPSF+NTR DFQVESRNYE EESESLKDQV
Sbjct: 4   VRGSSSWSEELASLVEDSGIRYVAEPIGISSPSFDNTRLDFQVESRNYESEESESLKDQV 63

Query: 121 KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRD 180
           KGFAVAWGEILLELGRGCRDIVQQNLITEDSYV +KLRGPCANVTARLSFLNEFLPEDRD
Sbjct: 64  KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVARKLRGPCANVTARLSFLNEFLPEDRD 123

Query: 181 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGV 240
           PVYAWPVIFFVSILAFTVI VNNRHESFSRPIMKVRDHPP ATRILLPDGRHMAYDVLGV
Sbjct: 124 PVYAWPVIFFVSILAFTVIFVNNRHESFSRPIMKVRDHPPSATRILLPDGRHMAYDVLGV 183

Query: 241 SADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSS 300
           SADRARFSIIAPHSFLSSR AGIPGVKTSLLEEFGVRLV+YDLPGFGESDPHPNRNLNSS
Sbjct: 184 SADRARFSIIAPHSFLSSRLAGIPGVKTSLLEEFGVRLVSYDLPGFGESDPHPNRNLNSS 243

Query: 301 AFDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTRE 360
           AFDMLHLADA+SI+GKFWVLG+SEGAMH WAALRYIPD  AG IMVAPMINPYEK +TRE
Sbjct: 244 AFDMLHLADAISISGKFWVLGHSEGAMHAWAALRYIPDEFAGTIMVAPMINPYEKIMTRE 303

Query: 361 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 420
           ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE
Sbjct: 304 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 363

Query: 421 DPKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSL 480
           DPKFREFWYRNVEESIRQKNVKPFIEET+L VSNWGFSLADLRVQRKCQR GIL WLKSL
Sbjct: 364 DPKFREFWYRNVEESIRQKNVKPFIEETVLQVSNWGFSLADLRVQRKCQRAGILPWLKSL 423

Query: 481 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDE 540
           YSQEECELAGFVGPIHIWQGIDD+VVPPSMTDYIGRILPAA+LHKLSNEGHFSFLYFCDE
Sbjct: 424 YSQEECELAGFVGPIHIWQGIDDQVVPPSMTDYIGRILPAAILHKLSNEGHFSFLYFCDE 483

Query: 541 CHRQIFSTIFGPPKGPVDRRERMEASPLEGNIGEVATTIDLTVK 585
           CHRQIFSTIFG PKGPVDRRERMEASP EGN  +VATT D  V+
Sbjct: 484 CHRQIFSTIFGIPKGPVDRRERMEASPFEGNTRDVATTTDPIVE 527

BLAST of Spg033500 vs. ExPASy TrEMBL
Match: A0A1S3B679 (uncharacterized protein LOC103486460 OS=Cucumis melo OX=3656 GN=LOC103486460 PE=4 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.6e-274
Identity = 465/522 (89.08%), Postives = 489/522 (93.68%), Query Frame = 0

Query: 64  SSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNY-EPEESESLKDQVKG 123
           SSSWSEE A L EDSGIRYVAEPIG+SSPSFENT S F VES NY E  ESESLK+QVKG
Sbjct: 5   SSSWSEEFAGLWEDSGIRYVAEPIGISSPSFENTGSIFPVESGNYKESVESESLKNQVKG 64

Query: 124 FAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRDPV 183
           FA+AWGEILLELGRGCRDIV+QNLITEDSYV QKLRGPCA+VT+RLSFLNEFLPEDRDPV
Sbjct: 65  FALAWGEILLELGRGCRDIVKQNLITEDSYV-QKLRGPCASVTSRLSFLNEFLPEDRDPV 124

Query: 184 YAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGVSA 243
           YAWPVIFFVSILAFTVICVNNR ESFSRPIMKVRDH P A+ ILLPDGRHMAYDV GVSA
Sbjct: 125 YAWPVIFFVSILAFTVICVNNRQESFSRPIMKVRDHLPSASLILLPDGRHMAYDVYGVSA 184

Query: 244 DRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSSAF 303
           DRARFSIIAPHSFLSSRFAGIPGVK SLLEEFGVRL+AYDLPGFGESDPHP+RNLNSSAF
Sbjct: 185 DRARFSIIAPHSFLSSRFAGIPGVKMSLLEEFGVRLIAYDLPGFGESDPHPHRNLNSSAF 244

Query: 304 DMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTREEL 363
           DMLHLADA+SI GKFWVLGYSEGAMH WAALRYIPDR+AGAIMVAPMINPYEK++TREEL
Sbjct: 245 DMLHLADAISINGKFWVLGYSEGAMHAWAALRYIPDRIAGAIMVAPMINPYEKSMTREEL 304

Query: 364 RRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIEDP 423
           RRTWENW PRKRLLYFLARRFPRFLSYFYRR+FLSG+HEEI+RQLSLSL KKDEVLIEDP
Sbjct: 305 RRTWENWAPRKRLLYFLARRFPRFLSYFYRRNFLSGRHEEIERQLSLSLRKKDEVLIEDP 364

Query: 424 KFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSLYS 483
           KFREFWYRNVEESIRQKNVKPF+EETMLLVSNWGFSLADLRVQRKCQR+ IL WLKSLYS
Sbjct: 365 KFREFWYRNVEESIRQKNVKPFVEETMLLVSNWGFSLADLRVQRKCQRSSILHWLKSLYS 424

Query: 484 QEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDECH 543
           Q++CELAGFVGPIHIWQGIDD+ VP SMTDYIGRILPAA+LHKLSNEGHFSF YFCDECH
Sbjct: 425 QDQCELAGFVGPIHIWQGIDDQAVPQSMTDYIGRILPAAVLHKLSNEGHFSFFYFCDECH 484

Query: 544 RQIFSTIFGPPKGPVDRRERMEASPLEGNIGEVATTIDLTVK 585
           RQIFSTIFGPPKGPVDR+ER+EASPLEGN       IDLTVK
Sbjct: 485 RQIFSTIFGPPKGPVDRKERIEASPLEGN-------IDLTVK 518

BLAST of Spg033500 vs. ExPASy TrEMBL
Match: A0A0A0LAD0 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G736880 PE=4 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.8e-273
Identity = 463/522 (88.70%), Postives = 487/522 (93.30%), Query Frame = 0

Query: 64  SSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNYEPE-ESESLKDQVKG 123
           SSSWSEE A L EDSGIRYVAEPIG+SSPS ENT S F VES NYE   ESESLK+QVKG
Sbjct: 5   SSSWSEEFAGLWEDSGIRYVAEPIGISSPSIENTGSVFPVESGNYEESVESESLKNQVKG 64

Query: 124 FAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRDPV 183
           FA+AWGEILLELGRGCRDIVQQNLITEDSY+ QKLRGPCA+VT+RLSFLNEFLPEDRDPV
Sbjct: 65  FALAWGEILLELGRGCRDIVQQNLITEDSYI-QKLRGPCASVTSRLSFLNEFLPEDRDPV 124

Query: 184 YAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGVSA 243
           YAWPVIFFVSILAFTVICVNNR ESFSRPIMKVRDH P A+ +LLPDGRHMAYDV GVSA
Sbjct: 125 YAWPVIFFVSILAFTVICVNNRQESFSRPIMKVRDHLPSASLMLLPDGRHMAYDVYGVSA 184

Query: 244 DRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSSAF 303
           DRARFSI+APHSFLSSR AGIPGVK SLLEEFGVRLVAYDLPGFGESDPHP+RNLNSSAF
Sbjct: 185 DRARFSILAPHSFLSSRLAGIPGVKMSLLEEFGVRLVAYDLPGFGESDPHPHRNLNSSAF 244

Query: 304 DMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTREEL 363
           DMLHLADA+SI GKFWVLGYSEGAMH WAALRYIPDR+AGAIMVAPMINPYEK +TREEL
Sbjct: 245 DMLHLADAISINGKFWVLGYSEGAMHAWAALRYIPDRIAGAIMVAPMINPYEKGMTREEL 304

Query: 364 RRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIEDP 423
           RRTWENWGPRKRLLYFLARRFPRFLSYFYRR+FLSG+HEEI+RQLSLSL KKDEVLIEDP
Sbjct: 305 RRTWENWGPRKRLLYFLARRFPRFLSYFYRRNFLSGRHEEIERQLSLSLRKKDEVLIEDP 364

Query: 424 KFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSLYS 483
           KF+EFWYRNVEESIRQKNVKPF+EETMLLVSNWGFSLADLRVQRKCQR+ IL WLKSLYS
Sbjct: 365 KFKEFWYRNVEESIRQKNVKPFVEETMLLVSNWGFSLADLRVQRKCQRSSILHWLKSLYS 424

Query: 484 QEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDECH 543
           QE+CELAGFVGPIHIWQGIDD+ VP SMTDYIGRILPAA+LHKLSNEGHFSF YFCDECH
Sbjct: 425 QEQCELAGFVGPIHIWQGIDDQAVPLSMTDYIGRILPAAVLHKLSNEGHFSFFYFCDECH 484

Query: 544 RQIFSTIFGPPKGPVDRRERMEASPLEGNIGEVATTIDLTVK 585
           RQIFSTIFGPPKGPVDR+ER+EASPLEGN       IDLTVK
Sbjct: 485 RQIFSTIFGPPKGPVDRKERIEASPLEGN-------IDLTVK 518

BLAST of Spg033500 vs. TAIR 10
Match: AT5G02970.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 636.3 bits (1640), Expect = 2.4e-182
Identity = 294/509 (57.76%), Postives = 393/509 (77.21%), Query Frame = 0

Query: 61  VLGSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSDFQVESRNYEPEESESLKDQV 120
           + G  +W EELASLV D+G+RY   PI +++ +    RS F V +     E  E+LKDQV
Sbjct: 4   IRGVPTWQEELASLV-DAGLRYDGAPIDLTAAT---KRSGF-VSADGSGSEPKETLKDQV 63

Query: 121 KGFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRD 180
            GF  +WGE+LLEL +GC+DIVQQ ++T+DS++ +KLR P A V+ +LSFLNEFLPEDRD
Sbjct: 64  TGFMKSWGEMLLELAKGCKDIVQQTVVTDDSFLVRKLRKPAAKVSKKLSFLNEFLPEDRD 123

Query: 181 PVYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGV 240
           P++AWPVIFFV +LA   +  +  ++     I K+R HP  ATR+ LPDGR++AY  LGV
Sbjct: 124 PIHAWPVIFFVFLLALAALSFSPENDRPVTVITKLRLHPTGATRVQLPDGRYIAYQELGV 183

Query: 241 SADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSS 300
           SA+RAR+S++ PHSFLSSR AGIPGVK SLL E+GVRLV+YDLPGFGESDPH  RNL+SS
Sbjct: 184 SAERARYSLVMPHSFLSSRLAGIPGVKKSLLVEYGVRLVSYDLPGFGESDPHRGRNLSSS 243

Query: 301 AFDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTRE 360
           A DM++LA A+ I  KFW+LGYS G++H WA ++Y P+++AGA MVAP+INPYE ++ +E
Sbjct: 244 ASDMINLAAAIGIDEKFWLLGYSTGSIHTWAGMKYFPEKIAGAAMVAPVINPYEPSMVKE 303

Query: 361 ELRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIE 420
           E+ +TWE W  +++ +YFLARRFP  L +FYRRSFLSG  +++D+ ++LSLG+KD++LI+
Sbjct: 304 EVVKTWEQWLTKRKFMYFLARRFPILLPFFYRRSFLSGNLDQLDQWMALSLGEKDKLLIK 363

Query: 421 DPKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSL 480
           DP F+E + RNVEES+RQ   KPF+EE +L VSNWGF+L++ R Q+KC   G+L+WL S+
Sbjct: 364 DPTFQEVYQRNVEESVRQGITKPFVEEAVLQVSNWGFTLSEFRTQKKCATNGVLSWLMSM 423

Query: 481 YSQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDE 540
           YS+ ECEL GF  PIHIWQG++D+V PPSM+DYI R++P A +HK+ NEGHFSF YFCDE
Sbjct: 424 YSEAECELIGFRKPIHIWQGMEDRVAPPSMSDYISRMIPEATVHKIRNEGHFSFFYFCDE 483

Query: 541 CHRQIFSTIFGPPKGPVDRRERMEASPLE 570
           CHRQIF  +FG PKG ++R +  E + +E
Sbjct: 484 CHRQIFYALFGEPKGQLERVKETEDTVVE 507

BLAST of Spg033500 vs. TAIR 10
Match: AT3G09690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 630.6 bits (1625), Expect = 1.3e-180
Identity = 290/501 (57.88%), Postives = 384/501 (76.65%), Query Frame = 0

Query: 63  GSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSD-FQVESRNYEPEESESLKDQVK 122
           G  +W +ELASL+ D G++Y   PI +++ +   ++S  F+  S +   E  ESLKDQV 
Sbjct: 7   GVPTWQDELASLM-DGGLQYDGSPIDLTADTESRSKSSGFESGSGS---ESVESLKDQVT 66

Query: 123 GFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRDP 182
           GF  +WGE+L++L  GC+D+VQQ ++T+DS+V +KLR P A V+ +LSFLNE+LPEDRDP
Sbjct: 67  GFMKSWGEMLMDLAIGCKDVVQQMVVTDDSFVVRKLRKPAAKVSKKLSFLNEYLPEDRDP 126

Query: 183 VYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGVS 242
           V+AWPVIFFV +LA T +  ++ H+     + K+R HP  A+R+ LPDGR++AY  LGVS
Sbjct: 127 VHAWPVIFFVFLLALTALSFSSDHDRSVPLLKKIRLHPTSASRVQLPDGRYLAYQELGVS 186

Query: 243 ADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSSA 302
           ADRAR S+I PHSFLSSR AGIPGVK SLL+++GVRLV+YDLPGFGESDPH  RNL+SSA
Sbjct: 187 ADRARHSLIVPHSFLSSRLAGIPGVKESLLKDYGVRLVSYDLPGFGESDPHRARNLSSSA 246

Query: 303 FDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTREE 362
            DM+ LA A+ I  KFW+LGYS G++H WAA+RY PD++AG  MVAPMINPYE ++T+EE
Sbjct: 247 SDMIDLAAALGIVDKFWLLGYSSGSVHAWAAMRYFPDQIAGVAMVAPMINPYEPSMTKEE 306

Query: 363 LRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIED 422
           + +TWE W  +++ +YFLARR+P  L + YRRSFLSG  E +D+ +S+SLG+KD+++  D
Sbjct: 307 MAKTWEQWQRKRKFMYFLARRWPSLLPFSYRRSFLSGNLEPLDKWMSVSLGEKDKLVTAD 366

Query: 423 PKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSLY 482
           P F + + RNVEES+RQ   KPF+EE  L VSNWGFSL +  +Q+KC+  G+L+WL S+Y
Sbjct: 367 PVFEDLYQRNVEESVRQGTAKPFVEEAALQVSNWGFSLPEFHMQKKCRTNGVLSWLMSMY 426

Query: 483 SQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDEC 542
           S+ ECEL GF  PIHIWQG+DD+V PPS+TDYI R++P A +H+L NEGHFS+ Y CDEC
Sbjct: 427 SESECELIGFRKPIHIWQGMDDRVTPPSVTDYISRVIPEATVHRLPNEGHFSYFYLCDEC 486

Query: 543 HRQIFSTIFGPPKGPVDRRER 563
           H QIFS IFG PKGPV+  E+
Sbjct: 487 HTQIFSAIFGEPKGPVELLEQ 503

BLAST of Spg033500 vs. TAIR 10
Match: AT3G09690.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 630.6 bits (1625), Expect = 1.3e-180
Identity = 290/501 (57.88%), Postives = 384/501 (76.65%), Query Frame = 0

Query: 63  GSSSWSEELASLVEDSGIRYVAEPIGVSSPSFENTRSD-FQVESRNYEPEESESLKDQVK 122
           G  +W +ELASL+ D G++Y   PI +++ +   ++S  F+  S +   E  ESLKDQV 
Sbjct: 7   GVPTWQDELASLM-DGGLQYDGSPIDLTADTESRSKSSGFESGSGS---ESVESLKDQVT 66

Query: 123 GFAVAWGEILLELGRGCRDIVQQNLITEDSYVGQKLRGPCANVTARLSFLNEFLPEDRDP 182
           GF  +WGE+L++L  GC+D+VQQ ++T+DS+V +KLR P A V+ +LSFLNE+LPEDRDP
Sbjct: 67  GFMKSWGEMLMDLAIGCKDVVQQMVVTDDSFVVRKLRKPAAKVSKKLSFLNEYLPEDRDP 126

Query: 183 VYAWPVIFFVSILAFTVICVNNRHESFSRPIMKVRDHPPCATRILLPDGRHMAYDVLGVS 242
           V+AWPVIFFV +LA T +  ++ H+     + K+R HP  A+R+ LPDGR++AY  LGVS
Sbjct: 127 VHAWPVIFFVFLLALTALSFSSDHDRSVPLLKKIRLHPTSASRVQLPDGRYLAYQELGVS 186

Query: 243 ADRARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSSA 302
           ADRAR S+I PHSFLSSR AGIPGVK SLL+++GVRLV+YDLPGFGESDPH  RNL+SSA
Sbjct: 187 ADRARHSLIVPHSFLSSRLAGIPGVKESLLKDYGVRLVSYDLPGFGESDPHRARNLSSSA 246

Query: 303 FDMLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTREE 362
            DM+ LA A+ I  KFW+LGYS G++H WAA+RY PD++AG  MVAPMINPYE ++T+EE
Sbjct: 247 SDMIDLAAALGIVDKFWLLGYSSGSVHAWAAMRYFPDQIAGVAMVAPMINPYEPSMTKEE 306

Query: 363 LRRTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIED 422
           + +TWE W  +++ +YFLARR+P  L + YRRSFLSG  E +D+ +S+SLG+KD+++  D
Sbjct: 307 MAKTWEQWQRKRKFMYFLARRWPSLLPFSYRRSFLSGNLEPLDKWMSVSLGEKDKLVTAD 366

Query: 423 PKFREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSLY 482
           P F + + RNVEES+RQ   KPF+EE  L VSNWGFSL +  +Q+KC+  G+L+WL S+Y
Sbjct: 367 PVFEDLYQRNVEESVRQGTAKPFVEEAALQVSNWGFSLPEFHMQKKCRTNGVLSWLMSMY 426

Query: 483 SQEECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGHFSFLYFCDEC 542
           S+ ECEL GF  PIHIWQG+DD+V PPS+TDYI R++P A +H+L NEGHFS+ Y CDEC
Sbjct: 427 SESECELIGFRKPIHIWQGMDDRVTPPSVTDYISRVIPEATVHRLPNEGHFSYFYLCDEC 486

Query: 543 HRQIFSTIFGPPKGPVDRRER 563
           H QIFS IFG PKGPV+  E+
Sbjct: 487 HTQIFSAIFGEPKGPVELLEQ 503

BLAST of Spg033500 vs. TAIR 10
Match: AT5G22460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 7.6e-40
Identity = 107/348 (30.75%), Postives = 169/348 (48.56%), Query Frame = 0

Query: 188 IFFVSILAFTVICVNNRHESFSRPIMKVRDHP--PCAT--RILLPDGRHMAYDVLGVSAD 247
           + F   +A  V  +   + SF  P  ++  HP  P  T  RI L DGR++AY   GV  D
Sbjct: 1   MMFSVTVAILVCLIGYIYRSFKPPPPRICGHPNGPPVTSPRIKLSDGRYLAYRESGVDRD 60

Query: 248 RARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSSAFD 307
            A + II  H F SS+    P +   ++EE G+  V YD  G+GESDPHP+R + S A+D
Sbjct: 61  NANYKIIVVHGFNSSKDTEFP-IPKDVIEELGIYFVFYDRAGYGESDPHPSRTVKSEAYD 120

Query: 308 MLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTREELR 367
           +  LAD + I  KF+VLG S GA  V++ L+YIP R+AGA+++ P +N +   + +E+L 
Sbjct: 121 IQELADKLKIGPKFYVLGISLGAYSVYSCLKYIPHRLAGAVLMVPFVNYWWTKVPQEKLS 180

Query: 368 RTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIEDPK 427
           +  E   P+K    F    +  +L Y++    L      +    +L    KD V+I+   
Sbjct: 181 KALELM-PKKDQWTFKVAHYVPWLLYWWLTQKLFPSSSMVTGNNAL-CSDKDLVVIKK-- 240

Query: 428 FREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSLYSQ 487
                        + +N +P +E+             D     +    G  TW +   ++
Sbjct: 241 -------------KMENPRPGLEKVR--------QQGDHECLHRDMIAGFATW-EFDPTE 300

Query: 488 EECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGH 532
            E   A   G +H+WQG++D+++P  +  YI   LP    H++   GH
Sbjct: 301 LENPFAEGEGSVHVWQGMEDRIIPYEINRYISEKLPWIKYHEVLGYGH 321

BLAST of Spg033500 vs. TAIR 10
Match: AT5G22460.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 7.6e-40
Identity = 107/348 (30.75%), Postives = 169/348 (48.56%), Query Frame = 0

Query: 188 IFFVSILAFTVICVNNRHESFSRPIMKVRDHP--PCAT--RILLPDGRHMAYDVLGVSAD 247
           + F   +A  V  +   + SF  P  ++  HP  P  T  RI L DGR++AY   GV  D
Sbjct: 1   MMFSVTVAILVCLIGYIYRSFKPPPPRICGHPNGPPVTSPRIKLSDGRYLAYRESGVDRD 60

Query: 248 RARFSIIAPHSFLSSRFAGIPGVKTSLLEEFGVRLVAYDLPGFGESDPHPNRNLNSSAFD 307
            A + II  H F SS+    P +   ++EE G+  V YD  G+GESDPHP+R + S A+D
Sbjct: 61  NANYKIIVVHGFNSSKDTEFP-IPKDVIEELGIYFVFYDRAGYGESDPHPSRTVKSEAYD 120

Query: 308 MLHLADAVSITGKFWVLGYSEGAMHVWAALRYIPDRVAGAIMVAPMINPYEKTLTREELR 367
           +  LAD + I  KF+VLG S GA  V++ L+YIP R+AGA+++ P +N +   + +E+L 
Sbjct: 121 IQELADKLKIGPKFYVLGISLGAYSVYSCLKYIPHRLAGAVLMVPFVNYWWTKVPQEKLS 180

Query: 368 RTWENWGPRKRLLYFLARRFPRFLSYFYRRSFLSGKHEEIDRQLSLSLGKKDEVLIEDPK 427
           +  E   P+K    F    +  +L Y++    L      +    +L    KD V+I+   
Sbjct: 181 KALELM-PKKDQWTFKVAHYVPWLLYWWLTQKLFPSSSMVTGNNAL-CSDKDLVVIKK-- 240

Query: 428 FREFWYRNVEESIRQKNVKPFIEETMLLVSNWGFSLADLRVQRKCQRTGILTWLKSLYSQ 487
                        + +N +P +E+             D     +    G  TW +   ++
Sbjct: 241 -------------KMENPRPGLEKVR--------QQGDHECLHRDMIAGFATW-EFDPTE 300

Query: 488 EECELAGFVGPIHIWQGIDDKVVPPSMTDYIGRILPAAMLHKLSNEGH 532
            E   A   G +H+WQG++D+++P  +  YI   LP    H++   GH
Sbjct: 301 LENPFAEGEGSVHVWQGMEDRIIPYEINRYISEKLPWIKYHEVLGYGH 321