Spg033371 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg033371
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	CASP-like protein
Location	scaffold5: 745451 .. 747025 (+)
RNA-Seq Expression	Spg033371
Synteny	Spg033371

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATCTCTCGATATTTTAATTGAAATCTAGCAAAAGAAAAAAAAAAAAACTATAAATGATTGACCATAAATGAAAATAGTATAAGAAACTTTCGTATACGATAACGTTTTCTAGCAGAGTAAATTTTCACATAGAAAAGGGAAAATTTCTAACTCCTACACCTGCTTCCGTTACAGATAAGCTGAGTTGTCGCTTCACAATACCACCAAAATATAATTAAAAGAAAAAAAAAGTTTAACAAATAACAAAAAACAAAAAAACAAATCCTATTTTCCACGAAAGAAAAAACTAAAAATTCTTTATCTTTACACGCCGATCTTCCAGTTCCAGTATCTGTTCCATCCAAAAGAAAGTCCCAATCACTAATCCTACGCTTCGATTTCGATCCTCATGTCAAATCCAGAAGACTCTGCAGCCAAGGAACAAAACGTGGCGCCGCCGTCTGCCCCCCCGGCGGCCGACGTCGAGAGCCAAACGCCACCGGCGACCGGATTCGGGGTGTCGGAGATCGTGAACCGGTGGCGGAGGGAGGATCTGTTAAAACGCCGCGTTTTGGCTCTCAGAGGATTTGGTCTGGTCTTTTCTTTGCTTGCTTTCTTTATTATGGCTTGCAACAAACATGGCAATTGGATGGATTTCGACAATTACGAAGAATTCAGGTAATCCGATTTCAATTTCAGTTGTTTTTAATTACGAATTTAGTTCGATTATCGACAGAGATTTGAATTTCGTATTGAAATAGGTACGTGCTGGCAATAGCGATTATGTCCACTTTGTATACGGGCGCCCAAGTTTATAGACAAGTTCATGAAGTTTCAACAGCCAGATCTATCTTTCCACCCAGAAAATCTGCATTTTTCGACTTTATTGGTGATCAGGTTGGTGTTTTTCTTCCAATCAAGGGCAAAGCAAAGAAAAGCCGAGTTAAATTATAAATTTGGTCCATAAGATTTTTAAAATTTTCAAATTAATCCCCAATAATTGGCCCTAATTTCAATGTTATCCCTACAAATCATTTATACCATTATTTGGATAGTTACATCAGTAGAGTAAAGAATAAAAGAAAATAGACTGGTCTTTCAAATAGGTCAAAATGCATTTTAGGATAGATATCTCCACAATATGATATTGTCCACTTTGGACGTAAACTTTCATGATTTTGCTTTTGTTCACCTCAAAAGACCTCATACCAATAGAGATACTTGTCCTCCCTTATAAGTCCATGATCCTCCCATTATCTAACCAATGTGGGACTTTGGTTGCACTTCCGACAGCCATTGAGATCAAATTGAAACTTTAAAAACTACGATGATCAAATTAAAATTAAATTCAAACCATCGAGACTAAATTAAAACTGAAAATACTAATAATTGAATGTGCAGTTTCTGGCTTATATGCTGATATCAGCGGCATCTTCAGCAGCTCCAATGACAAACAGAATGAGAGCAGACAGAGCCAACATTTTTACAGACTCATTGGCAGCTTCAATTACCATGTCCTTTTTCGCCTTCTTGTCCTTAGCTTTATCGGCCGGCATTACAGGATACAAACTCTCAACTCAATCTTATATCTAA

mRNA sequence

ATGTCAAATCCAGAAGACTCTGCAGCCAAGGAACAAAACGTGGCGCCGCCGTCTGCCCCCCCGGCGGCCGACGTCGAGAGCCAAACGCCACCGGCGACCGGATTCGGGGTGTCGGAGATCGTGAACCGGTGGCGGAGGGAGGATCTGTTAAAACGCCGCGTTTTGGCTCTCAGAGGATTTGGTCTGGTCTTTTCTTTGCTTGCTTTCTTTATTATGGCTTGCAACAAACATGGCAATTGGATGGATTTCGACAATTACGAAGAATTCAGGTACGTGCTGGCAATAGCGATTATGTCCACTTTGTATACGGGCGCCCAAGTTTATAGACAAGTTCATGAAGTTTCAACAGCCAGATCTATCTTTCCACCCAGAAAATCTGCATTTTTCGACTTTATTGGTGATCAGTTTCTGGCTTATATGCTGATATCAGCGGCATCTTCAGCAGCTCCAATGACAAACAGAATGAGAGCAGACAGAGCCAACATTTTTACAGACTCATTGGCAGCTTCAATTACCATGTCCTTTTTCGCCTTCTTGTCCTTAGCTTTATCGGCCGGCATTACAGGATACAAACTCTCAACTCAATCTTATATCTAA

Coding sequence (CDS)

ATGTCAAATCCAGAAGACTCTGCAGCCAAGGAACAAAACGTGGCGCCGCCGTCTGCCCCCCCGGCGGCCGACGTCGAGAGCCAAACGCCACCGGCGACCGGATTCGGGGTGTCGGAGATCGTGAACCGGTGGCGGAGGGAGGATCTGTTAAAACGCCGCGTTTTGGCTCTCAGAGGATTTGGTCTGGTCTTTTCTTTGCTTGCTTTCTTTATTATGGCTTGCAACAAACATGGCAATTGGATGGATTTCGACAATTACGAAGAATTCAGGTACGTGCTGGCAATAGCGATTATGTCCACTTTGTATACGGGCGCCCAAGTTTATAGACAAGTTCATGAAGTTTCAACAGCCAGATCTATCTTTCCACCCAGAAAATCTGCATTTTTCGACTTTATTGGTGATCAGTTTCTGGCTTATATGCTGATATCAGCGGCATCTTCAGCAGCTCCAATGACAAACAGAATGAGAGCAGACAGAGCCAACATTTTTACAGACTCATTGGCAGCTTCAATTACCATGTCCTTTTTCGCCTTCTTGTCCTTAGCTTTATCGGCCGGCATTACAGGATACAAACTCTCAACTCAATCTTATATCTAA

Protein sequence

MSNPEDSAAKEQNVAPPSAPPAADVESQTPPATGFGVSEIVNRWRREDLLKRRVLALRGFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAFLSLALSAGITGYKLSTQSYI

Homology

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BLAST of Spg033371 vs. NCBI nr
Match: KAG6596321.1 (CASP-like protein 4B1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 298.9 bits (764), Expect = 3.2e-77
Identity = 161/214 (75.23%), Postives = 173/214 (80.84%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVA----------------PPSAPPAADVESQTPPATGFGVSEIVNRW 60
           MSNPEDS  K++N A                PPSA P  DVE QTP AT  GVSEI+NRW
Sbjct: 1   MSNPEDSPTKQKNGAPPPSVPPTVNVERQSPPPSANPTPDVEMQTPAATSSGVSEILNRW 60

Query: 61  RREDLLKRRVLALRGFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTG 120
            REDLLKRRVLALRGFGLVFSLLAF IMACN+HG+W +FDNYEEFRYVLAIAI+ST YTG
Sbjct: 61  GREDLLKRRVLALRGFGLVFSLLAFLIMACNRHGDWKNFDNYEEFRYVLAIAILSTFYTG 120

Query: 121 AQVYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFT 180
           AQV+RQ+HEVSTARS+FPPRKSA  DFIGDQ LAY+LISA+SSA PM NRMR    NIFT
Sbjct: 121 AQVFRQIHEVSTARSMFPPRKSAIIDFIGDQILAYLLISASSSAIPMINRMREGSDNIFT 180

Query: 181 DSLAASITMSFFAFLSLALSAGITGYKLSTQSYI 199
           DSLAASITMS FAFLSLALSA ITGYKLSTQSYI
Sbjct: 181 DSLAASITMSLFAFLSLALSAAITGYKLSTQSYI 214

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BLAST of Spg033371 vs. NCBI nr
Match: XP_022951298.1 (CASP-like protein 4B1 [Cucurbita moschata])

HSP 1 Score: 298.9 bits (764), Expect = 3.2e-77
Identity = 161/214 (75.23%), Postives = 173/214 (80.84%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVA----------------PPSAPPAADVESQTPPATGFGVSEIVNRW 60
           MSNPEDS  K++N A                PPSA P  DVE QTP AT  GVSEI+NRW
Sbjct: 1   MSNPEDSPTKQKNGAPPPSVPPTVNVGRQSPPPSANPTPDVEMQTPAATSSGVSEILNRW 60

Query: 61  RREDLLKRRVLALRGFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTG 120
            REDLLKRRVLALRGFGLVFSLLAF IMACN+HG+W +FDNYEEFRYVLAIAI+ST YTG
Sbjct: 61  GREDLLKRRVLALRGFGLVFSLLAFLIMACNRHGDWKNFDNYEEFRYVLAIAILSTFYTG 120

Query: 121 AQVYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFT 180
           AQV+RQ+HEVSTARS+FPPRKSA  DFIGDQ LAY+LISA+SSA PM NRMR    NIFT
Sbjct: 121 AQVFRQIHEVSTARSMFPPRKSAIIDFIGDQILAYLLISASSSAIPMINRMREGSDNIFT 180

Query: 181 DSLAASITMSFFAFLSLALSAGITGYKLSTQSYI 199
           DSLAASITMS FAFLSLALSA ITGYKLSTQSYI
Sbjct: 181 DSLAASITMSLFAFLSLALSAAITGYKLSTQSYI 214

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BLAST of Spg033371 vs. NCBI nr
Match: KAG7027874.1 (CASP-like protein 4B1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 296.2 bits (757), Expect = 2.1e-76
Identity = 160/214 (74.77%), Postives = 172/214 (80.37%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVA----------------PPSAPPAADVESQTPPATGFGVSEIVNRW 60
           MSNPEDS  K++N A                PPSA P  DVE QTP AT  GVSEI+NRW
Sbjct: 1   MSNPEDSPTKQKNGAPPPSVPPTVNVERQSPPPSANPTPDVEMQTPAATSSGVSEILNRW 60

Query: 61  RREDLLKRRVLALRGFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTG 120
            REDLLKRRVLALRGFGLVFSLLAF IMACN+HG+W +FDNYEEFRYVLAIAI+ST YTG
Sbjct: 61  GREDLLKRRVLALRGFGLVFSLLAFLIMACNRHGDWKNFDNYEEFRYVLAIAILSTFYTG 120

Query: 121 AQVYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFT 180
           AQV+RQ+HEVSTARS+FPP KSA  DFIGDQ LAY+LISA+SSA PM NRMR    NIFT
Sbjct: 121 AQVFRQIHEVSTARSMFPPPKSAIIDFIGDQILAYLLISASSSAIPMINRMREGSDNIFT 180

Query: 181 DSLAASITMSFFAFLSLALSAGITGYKLSTQSYI 199
           DSLAASITMS FAFLSLALSA ITGYKLSTQSYI
Sbjct: 181 DSLAASITMSLFAFLSLALSAAITGYKLSTQSYI 214

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BLAST of Spg033371 vs. NCBI nr
Match: XP_022971522.1 (CASP-like protein 4B1 [Cucurbita maxima])

HSP 1 Score: 295.8 bits (756), Expect = 2.7e-76
Identity = 159/214 (74.30%), Postives = 172/214 (80.37%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVA----------------PPSAPPAADVESQTPPATGFGVSEIVNRW 60
           MSNPEDSA K++N A                PPSA P  DVE Q P AT  GVSEI++RW
Sbjct: 1   MSNPEDSATKQKNGAPPPSALPTVNVERQSPPPSADPTPDVEMQAPAATSSGVSEILSRW 60

Query: 61  RREDLLKRRVLALRGFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTG 120
            REDLLKRRVLALRGFGLVFSLLAF IMACN+HG+W +FDNYEEFRYVLAIAI+ST YTG
Sbjct: 61  GREDLLKRRVLALRGFGLVFSLLAFLIMACNRHGDWKNFDNYEEFRYVLAIAILSTFYTG 120

Query: 121 AQVYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFT 180
           AQV+RQ+HEVSTARS+FPPRKSA  DFIGDQ LAY+LISA+SSA PM NRMR    NIFT
Sbjct: 121 AQVFRQIHEVSTARSMFPPRKSAIIDFIGDQILAYLLISASSSAIPMINRMREGSDNIFT 180

Query: 181 DSLAASITMSFFAFLSLALSAGITGYKLSTQSYI 199
           DSLAASITMS FAFLSLALSA ITGYK STQSYI
Sbjct: 181 DSLAASITMSLFAFLSLALSAAITGYKFSTQSYI 214

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BLAST of Spg033371 vs. NCBI nr
Match: XP_023538779.1 (CASP-like protein 4B1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 287.3 bits (734), Expect = 9.7e-74
Identity = 155/210 (73.81%), Postives = 169/210 (80.48%), Query Frame = 0


Query: 1   MSNPEDSAAKE------------QNVAPPSAPPAADVESQTPPATGFGVSEIVNRWRRED 60
           MSNPEDSA K+            +   PPSA P  DVE QTP AT  GVSEI++RW RED
Sbjct: 1   MSNPEDSATKQTAPSALPTVNVGRQSPPPSANPTPDVEMQTPAATSSGVSEILSRWGRED 60

Query: 61  LLKRRVLALRGFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVY 120
           LLKRRVLALRGFGLVFSLLAF IMACN+HG+W +FD YEE RYVLAIAI+ST YTGAQV+
Sbjct: 61  LLKRRVLALRGFGLVFSLLAFLIMACNRHGDWKNFDKYEEIRYVLAIAILSTFYTGAQVF 120

Query: 121 RQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLA 180
           RQ+HEVSTA S+FPPRKSA  DFIGDQ LAY+LISA+SSA PM NRMR    NIFTDSLA
Sbjct: 121 RQIHEVSTAISMFPPRKSAIIDFIGDQILAYLLISASSSAIPMINRMREGSDNIFTDSLA 180

Query: 181 ASITMSFFAFLSLALSAGITGYKLSTQSYI 199
           ASI+MS FAFLSLALSA ITGYKLSTQSYI
Sbjct: 181 ASISMSLFAFLSLALSAAITGYKLSTQSYI 210

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BLAST of Spg033371 vs. ExPASy Swiss-Prot
Match: D7LBN4 (CASP-like protein 4B1 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_321547 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.7e-41
Identity = 101/198 (51.01%), Postives = 131/198 (66.16%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVAPPSAPPAADVESQTPPATGFGVSEIVNRWRREDLLKRRVLALRGF 60
           M+NP+    K++ V       AA+  S+  PA+  G S I  RW+REDL+K+     RG 
Sbjct: 1   MTNPD----KQKPVEVTDVETAAEKTSEPTPAS--GTSTITQRWKREDLIKKASPITRGI 60

Query: 61  GLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTARSI 120
            L+FSLLAF IM  NKHG   +F+ YEE+RYVLAI+I+STLYT  Q +       + R  
Sbjct: 61  CLLFSLLAFLIMVSNKHGYGRNFNEYEEYRYVLAISIISTLYTAWQTFAHF----SKREF 120

Query: 121 FPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAFLS 180
           F  R S   DF GDQ +AY+LISAASSA P+TNR R  + NIFTDS A++I+M+ FAF++
Sbjct: 121 FDRRTSTLVDFSGDQIVAYLLISAASSAIPLTNRFREGQDNIFTDSAASAISMAIFAFVA 180

Query: 181 LALSAGITGYKLSTQSYI 199
           LALSA  +GYKLST S+I
Sbjct: 181 LALSALFSGYKLSTHSFI 188

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BLAST of Spg033371 vs. ExPASy Swiss-Prot
Match: Q8LE26 (CASP-like protein 4B1 OS=Arabidopsis thaliana OX=3702 GN=At2g38480 PE=2 SV=2)

HSP 1 Score: 166.0 bits (419), Expect = 4.3e-40
Identity = 99/198 (50.00%), Postives = 131/198 (66.16%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVAPPSAPPAADVESQTPPATGFGVSEIVNRWRREDLLKRRVLALRGF 60
           M+NP++     + V       AA+  S+  PA+  G S I  RW+REDL+K+     RG 
Sbjct: 1   MTNPDNM----KPVEATDVESAAEKTSEPTPAS--GTSTITQRWKREDLIKKASPITRGI 60

Query: 61  GLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTARSI 120
            L+FSL+AF IM  NKHG   +F++YEE+RYVLAI+I+STLYT  Q +       + R I
Sbjct: 61  CLLFSLIAFLIMVSNKHGYGRNFNDYEEYRYVLAISIISTLYTAWQTFAHF----SKREI 120

Query: 121 FPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAFLS 180
           F  R S   DF GDQ +AY+LISAASSA P+TN  R  + NIFTDS A++I+M+ FAF++
Sbjct: 121 FDRRTSILVDFSGDQIVAYLLISAASSAIPLTNIFREGQDNIFTDSAASAISMAIFAFIA 180

Query: 181 LALSAGITGYKLSTQSYI 199
           LALSA  +GYKLST S+I
Sbjct: 181 LALSALFSGYKLSTHSFI 188

[Close]

BLAST of Spg033371 vs. ExPASy Swiss-Prot
Match: Q5W6M3 (CASP-like protein 4B4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0344400 PE=3 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 6.9e-38
Identity = 92/193 (47.67%), Postives = 128/193 (66.32%), Query Frame = 0


Query: 7   SAAKEQNVAPPSAPPAADVESQTPPATGFG-VSEIVNRWRREDLLKRRVLALRGFGLVFS 66
           +AA + NV    AP AA        A+G G VS +V RWRR+DLL++   ALR     FS
Sbjct: 20  AAAADANVDGGGAPAAA-------AASGEGVVSAVVRRWRRQDLLEKSGSALRVAAWAFS 79

Query: 67  LLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTARSIFPPRK 126
           LLAF +M  N HG+W  F++YEE+RYV+AI +++ +YT  Q+ R    ++  + +   + 
Sbjct: 80  LLAFVVMGANDHGDWRQFEHYEEYRYVVAIGVLAFIYTTLQLVRHGVRLTGGQDL-QGKV 139

Query: 127 SAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAFLSLALSA 186
           +   DF GDQ  AY+L+SA S+A P+TNRMR    N+FTDS AASI+M+FFAFL LALSA
Sbjct: 140 AVLVDFAGDQVTAYLLMSAVSAAIPITNRMREGADNVFTDSSAASISMAFFAFLCLALSA 199

Query: 187 GITGYKLSTQSYI 199
            ++G+KL+ Q+YI
Sbjct: 200 LVSGFKLAKQTYI 204

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BLAST of Spg033371 vs. ExPASy Swiss-Prot
Match: C5WNF5 (CASP-like protein 4B1 OS=Sorghum bicolor OX=4558 GN=Sb01g038100 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 7.6e-37
Identity = 85/202 (42.08%), Postives = 124/202 (61.39%), Query Frame = 0


Query: 7   SAAKEQNVAPPSAPPAADVESQTPPATGFG----------VSEIVNRWRREDLLKRRVLA 66
           +A K Q+V  P   P     +     TG            V  +V RWRRED+L +  LA
Sbjct: 19  AAEKPQDVEKPDYAPYNGASTTADGGTGARARRGDGGGGVVDSVVARWRREDMLDKSPLA 78

Query: 67  LRGFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVST 126
           L     +F+ +A  ++A N+HG+WM FD Y+E+RY+LAIA ++ LY+ AQ  R  H +  
Sbjct: 79  LHAAAAIFAFVALVLVASNQHGDWMQFDRYQEYRYLLAIASLALLYSLAQAARHAHRMRG 138

Query: 127 ARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFF 186
                    +   DF+GDQ +AY+L+SA S+A P+TNRMR+   N FTD+ AA+I+M+FF
Sbjct: 139 GVDPVSSASARLLDFVGDQVVAYLLMSALSAAVPITNRMRSAVVNNFTDATAAAISMAFF 198

Query: 187 AFLSLALSAGITGYKLSTQSYI 199
           +F++LALSA ++GYKLS Q+Y+
Sbjct: 199 SFVALALSAVVSGYKLSKQTYM 220

[Close]

BLAST of Spg033371 vs. ExPASy Swiss-Prot
Match: F2D276 (CASP-like protein 4B2 OS=Hordeum vulgare subsp. vulgare OX=112509 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 6.4e-36
Identity = 87/197 (44.16%), Postives = 122/197 (61.93%), Query Frame = 0


Query: 11  EQNVAPPSAPPAADVE--------SQTPPATGFGVSE-IVNRWRREDLLKRRVLALRGFG 70
           + + A  +A    DVE        + T  A G GV E +V RWRRED+L +  LAL    
Sbjct: 7   DASAAADAATKQPDVEKDYSSYNGASTAGAGGGGVVESVVARWRREDMLDKCPLALHAAA 66

Query: 71  LVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTARSIF 130
             F+ +A  ++A N+HG+WM FD Y+E+ Y+LAIA ++  Y+ AQ  R  H +       
Sbjct: 67  AAFAFVALVLVASNQHGDWMQFDRYQEYMYLLAIAALAFAYSLAQALRHAHRMRGGADPI 126

Query: 131 PPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAFLSL 190
           P   +  FDFI DQ +AY+L+SA S+A P+TNRMR    N FTD+ AA+I+M+F AF++L
Sbjct: 127 PAPSARLFDFIADQVVAYLLMSALSAAIPITNRMRTAVINNFTDATAAAISMAFLAFVAL 186

Query: 191 ALSAGITGYKLSTQSYI 199
           ALSA ++GYKLS Q Y+
Sbjct: 187 ALSATVSGYKLSRQMYM 203

[Close]

BLAST of Spg033371 vs. ExPASy TrEMBL
Match: A0A6J1GHC2 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111454172 PE=3 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.6e-77
Identity = 161/214 (75.23%), Postives = 173/214 (80.84%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVA----------------PPSAPPAADVESQTPPATGFGVSEIVNRW 60
           MSNPEDS  K++N A                PPSA P  DVE QTP AT  GVSEI+NRW
Sbjct: 1   MSNPEDSPTKQKNGAPPPSVPPTVNVGRQSPPPSANPTPDVEMQTPAATSSGVSEILNRW 60

Query: 61  RREDLLKRRVLALRGFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTG 120
            REDLLKRRVLALRGFGLVFSLLAF IMACN+HG+W +FDNYEEFRYVLAIAI+ST YTG
Sbjct: 61  GREDLLKRRVLALRGFGLVFSLLAFLIMACNRHGDWKNFDNYEEFRYVLAIAILSTFYTG 120

Query: 121 AQVYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFT 180
           AQV+RQ+HEVSTARS+FPPRKSA  DFIGDQ LAY+LISA+SSA PM NRMR    NIFT
Sbjct: 121 AQVFRQIHEVSTARSMFPPRKSAIIDFIGDQILAYLLISASSSAIPMINRMREGSDNIFT 180

Query: 181 DSLAASITMSFFAFLSLALSAGITGYKLSTQSYI 199
           DSLAASITMS FAFLSLALSA ITGYKLSTQSYI
Sbjct: 181 DSLAASITMSLFAFLSLALSAAITGYKLSTQSYI 214

[Close]

BLAST of Spg033371 vs. ExPASy TrEMBL
Match: A0A6J1I265 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111470217 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 1.3e-76
Identity = 159/214 (74.30%), Postives = 172/214 (80.37%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVA----------------PPSAPPAADVESQTPPATGFGVSEIVNRW 60
           MSNPEDSA K++N A                PPSA P  DVE Q P AT  GVSEI++RW
Sbjct: 1   MSNPEDSATKQKNGAPPPSALPTVNVERQSPPPSADPTPDVEMQAPAATSSGVSEILSRW 60

Query: 61  RREDLLKRRVLALRGFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTG 120
            REDLLKRRVLALRGFGLVFSLLAF IMACN+HG+W +FDNYEEFRYVLAIAI+ST YTG
Sbjct: 61  GREDLLKRRVLALRGFGLVFSLLAFLIMACNRHGDWKNFDNYEEFRYVLAIAILSTFYTG 120

Query: 121 AQVYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFT 180
           AQV+RQ+HEVSTARS+FPPRKSA  DFIGDQ LAY+LISA+SSA PM NRMR    NIFT
Sbjct: 121 AQVFRQIHEVSTARSMFPPRKSAIIDFIGDQILAYLLISASSSAIPMINRMREGSDNIFT 180

Query: 181 DSLAASITMSFFAFLSLALSAGITGYKLSTQSYI 199
           DSLAASITMS FAFLSLALSA ITGYK STQSYI
Sbjct: 181 DSLAASITMSLFAFLSLALSAAITGYKFSTQSYI 214

[Close]

BLAST of Spg033371 vs. ExPASy TrEMBL
Match: A0A0A0L9S4 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G730790 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 8.1e-74
Identity = 147/198 (74.24%), Postives = 168/198 (84.85%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVAPPSAPPAADVESQTPPATGFGVSEIVNRWRREDLLKRRVLALRGF 60
           MSNPEDSA  +QNV+PPS PPAA+ +SQ+PPA+ FGVSEIV+RWRRED+LKRR LALRGF
Sbjct: 1   MSNPEDSAPTQQNVSPPSTPPAAESKSQSPPASSFGVSEIVSRWRREDVLKRRALALRGF 60

Query: 61  GLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTARSI 120
             +FSLLAF IMA NKHG+W DFD YEEFRYVLAI I+STLYTGAQV RQ HE+ST +S+
Sbjct: 61  AFIFSLLAFVIMASNKHGDWKDFDKYEEFRYVLAIEILSTLYTGAQVLRQFHELSTGKSV 120

Query: 121 FPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAFLS 180
             P+KS F DFIGDQ LAY+ +SAASSA PMTNRMR    + FTDSLAAS+TMSFFAFLS
Sbjct: 121 ILPQKSVFIDFIGDQSLAYLQMSAASSAVPMTNRMREGSDSSFTDSLAASVTMSFFAFLS 180

Query: 181 LALSAGITGYKLSTQSYI 199
           LALS+ I+GYKLST SYI
Sbjct: 181 LALSSTISGYKLSTHSYI 198

[Close]

BLAST of Spg033371 vs. ExPASy TrEMBL
Match: A0A6J1DT05 (CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111024030 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 8.1e-74
Identity = 152/200 (76.00%), Postives = 168/200 (84.00%), Query Frame = 0


Query: 1   MSNPEDSAAKEQN--VAPPSAPPAADVESQTPPATGFGVSEIVNRWRREDLLKRRVLALR 60
           MSNPED+A K++N  VA  +APPAADVESQT  ATGFGVSEIV+RWRREDLL+RR LALR
Sbjct: 1   MSNPEDTAPKQENGAVAAATAPPAADVESQTTAATGFGVSEIVSRWRREDLLRRRALALR 60

Query: 61  GFGLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTAR 120
           G GL+ SLLAF +MACNKHG+W DFD YEEFRY+LAIAI+STLYTG QV RQV E+ST  
Sbjct: 61  GCGLILSLLAFVVMACNKHGDWKDFDKYEEFRYLLAIAILSTLYTGVQVSRQVRELSTGT 120

Query: 121 SIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAF 180
           S+FP RKSA  DFIGDQ LAY+L+SAASSA PMTNRMR    NIFTDSL ASI MSFFAF
Sbjct: 121 SMFPLRKSAIIDFIGDQILAYLLMSAASSAIPMTNRMREGSDNIFTDSLVASICMSFFAF 180

Query: 181 LSLALSAGITGYKLSTQSYI 199
            SLA SA I+GYKLSTQSYI
Sbjct: 181 FSLAFSAAISGYKLSTQSYI 200

[Close]

BLAST of Spg033371 vs. ExPASy TrEMBL
Match: A0A5A7TQT0 (CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005660 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 2.9e-71
Identity = 144/198 (72.73%), Postives = 166/198 (83.84%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVAPPSAPPAADVESQTPPATGFGVSEIVNRWRREDLLKRRVLALRGF 60
           MSNPEDSA  +Q+V+ P APPAAD++SQ+  A+ FGVSEIV+RWRRED+LKRR LALRGF
Sbjct: 1   MSNPEDSAPTQQDVSLPPAPPAADLKSQSSAASSFGVSEIVSRWRREDILKRRALALRGF 60

Query: 61  GLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTARSI 120
             +FSLLAF IMA NKHG+W DFD YEEFRYVLAI I+STLYTGAQV RQ HE+ST +S+
Sbjct: 61  AFIFSLLAFIIMASNKHGDWKDFDKYEEFRYVLAIEILSTLYTGAQVLRQFHELSTGKSV 120

Query: 121 FPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAFLS 180
           F P+KS F DFIGDQ  AY+ +SAASSA PMTNRMR    + FTDSLAAS+TMSFFAFLS
Sbjct: 121 FLPQKSVFIDFIGDQSAAYLQMSAASSAVPMTNRMREGSDSFFTDSLAASVTMSFFAFLS 180

Query: 181 LALSAGITGYKLSTQSYI 199
           LALS+ I+GYKLST SYI
Sbjct: 181 LALSSIISGYKLSTHSYI 198

[Close]

BLAST of Spg033371 vs. TAIR 10
Match: AT2G38480.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 166.0 bits (419), Expect = 3.1e-41
Identity = 99/198 (50.00%), Postives = 131/198 (66.16%), Query Frame = 0


Query: 1   MSNPEDSAAKEQNVAPPSAPPAADVESQTPPATGFGVSEIVNRWRREDLLKRRVLALRGF 60
           M+NP++     + V       AA+  S+  PA+  G S I  RW+REDL+K+     RG 
Sbjct: 1   MTNPDNM----KPVEATDVESAAEKTSEPTPAS--GTSTITQRWKREDLIKKASPITRGI 60

Query: 61  GLVFSLLAFFIMACNKHGNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTARSI 120
            L+FSL+AF IM  NKHG   +F++YEE+RYVLAI+I+STLYT  Q +       + R I
Sbjct: 61  CLLFSLIAFLIMVSNKHGYGRNFNDYEEYRYVLAISIISTLYTAWQTFAHF----SKREI 120

Query: 121 FPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAFLS 180
           F  R S   DF GDQ +AY+LISAASSA P+TN  R  + NIFTDS A++I+M+ FAF++
Sbjct: 121 FDRRTSILVDFSGDQIVAYLLISAASSAIPLTNIFREGQDNIFTDSAASAISMAIFAFIA 180

Query: 181 LALSAGITGYKLSTQSYI 199
           LALSA  +GYKLST S+I
Sbjct: 181 LALSALFSGYKLSTHSFI 188

[Close]

BLAST of Spg033371 vs. TAIR 10
Match: AT2G36330.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 90.5 bits (223), Expect = 1.6e-18
Identity = 56/162 (34.57%), Postives = 94/162 (58.02%), Query Frame = 0


Query: 37  VSEIVNRWRREDLLKRRVLALRGFGLVFSLLAFFIMACNKHGNWM--DFDNYEEFRYVLA 96
           VS I+ R RRE+++K   L  R   +V +L++F IMA +K   W    FD Y+E+R+ L+
Sbjct: 119 VSAILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLS 178

Query: 97  IAIMSTLYTGAQVYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNR 156
           + +++ +Y+  Q     + +   + +        F+FI DQ LAY+L+SA+++A    + 
Sbjct: 179 VNVVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAVTRVDD 238

Query: 157 MRAD-RANIFTDSLAASITMSFFAFLSLALSAGITGYKLSTQ 196
             ++   + FT+  +ASI MSF AFL+ A S+ I+GY L  Q
Sbjct: 239 WVSNWGKDEFTEMASASIAMSFLAFLAFAFSSLISGYNLFNQ 280

[Close]

BLAST of Spg033371 vs. TAIR 10
Match: AT5G62820.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 73.2 bits (178), Expect = 2.7e-13
Identity = 51/159 (32.08%), Postives = 85/159 (53.46%), Query Frame = 0


Query: 41  VNRWRREDLLKRRVLALRGFGLVFSLLAFFIMACNKHGNWM--DFDNYEEFRYVLAIAIM 100
           VNR R +DL+    L  R   ++  +++F IMA +K   W    +D Y+E+RY LA+ ++
Sbjct: 137 VNRARGDDLVSLTALGFRITEVILCVISFSIMAADKTQGWSGDSYDRYKEYRYCLAVNVI 196

Query: 101 STLYTGAQVYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRAD 160
           + +Y+  +       ++    +        F F  DQ LAY+L+SA+S AA   +   ++
Sbjct: 197 AFVYSAFEACDAACYIAKESYMINCGFHDLFVFSMDQLLAYLLMSASSCAATRVDDWVSN 256

Query: 161 -RANIFTDSLAASITMSFFAFLSLALSAGITGYKLSTQS 197
              + FT    ASI +SF AF + A+SA I+ Y+L T +
Sbjct: 257 WGKDEFTQMATASIAVSFLAFGAFAVSALISSYRLFTHA 295

[Close]

BLAST of Spg033371 vs. TAIR 10
Match: AT4G11655.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 67.0 bits (162), Expect = 1.9e-11
Identity = 50/180 (27.78%), Postives = 85/180 (47.22%), Query Frame = 0


Query: 22  AADVESQTPPATGFGVSEIVNRW-RREDLLKRRVLALRGFGLVFSLLAFFIMACN----- 81
           A+ V  QTP    + V+   +R+  R   +    L LR   +V   ++   +A N     
Sbjct: 23  ASPVSQQTPSLFAYSVTPSASRFSSRRASVHVIGLVLRFITMVLCFVSALSLAVNVQRPS 82

Query: 82  -KH--GNWMDFDNYEEFRYVLAIAIMSTLYTGAQVYRQVHEVSTARSIFPPRKSAFFDFI 141
            +H   N   F +Y E  Y   +A++  +YT  Q ++ V +++    +     S +  FI
Sbjct: 83  KRHLTQNSSSFASYPELLYCFGVAVIGFVYTSLQTFKGVCDITHRGVLISEPLSDYISFI 142

Query: 142 GDQFLAYMLISAASSAAPMTNRMRADRANIFTDSLAASITMSFFAFLSLALSAGITGYKL 193
            DQ + Y+L+S++S A      +  D      ++   S++MSF AFL L LS  ++GYKL
Sbjct: 143 FDQVICYLLVSSSSVAIAWIQHINEDAIKTLRNNSIVSVSMSFSAFLVLTLSGLLSGYKL 202

[Close]

BLAST of Spg033371 vs. TAIR 10
Match: AT5G40300.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 65.9 bits (159), Expect = 4.3e-11
Identity = 44/147 (29.93%), Postives = 81/147 (55.10%), Query Frame = 0


Query: 49  LLKRRVLALRGFGLVFSLLAFFIMACNKHGNWM--DFDNYEEFRYVLAIAIMSTLYTGAQ 108
           L+++ +L  R    V  L++F +M  ++   W    F NY+EFR+ LA  ++  +Y+G  
Sbjct: 118 LIRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDSFYNYKEFRFCLAANVIGFVYSGFM 177

Query: 109 VYRQVHEVSTARSIFPPRKSAFFDFIGDQFLAYMLISAASSAAPMTNRMRAD-RANIFTD 168
           +   V+ +ST+          F +F  DQ LAY+L SA++SA+   +  +++  A+ F D
Sbjct: 178 ICDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLASASTSASIRVDDWQSNWGADKFPD 237

Query: 169 SLAASITMSFFAFLSLALSAGITGYKL 193
              AS+ +S+ +F++ A  +  +GY L
Sbjct: 238 LARASVALSYVSFVAFAFCSLASGYAL 264

The following BLAST results are available for this feature:

BLAST of Spg033371 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6596321.1	3.2e-77	75.23	CASP-like protein 4B1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022951298.1	3.2e-77	75.23	CASP-like protein 4B1 [Cucurbita moschata]	[more]
KAG7027874.1	2.1e-76	74.77	CASP-like protein 4B1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022971522.1	2.7e-76	74.30	CASP-like protein 4B1 [Cucurbita maxima]	[more]
XP_023538779.1	9.7e-74	73.81	CASP-like protein 4B1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg033371 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D7LBN4	1.7e-41	51.01	CASP-like protein 4B1 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT...	[more]
Q8LE26	4.3e-40	50.00	CASP-like protein 4B1 OS=Arabidopsis thaliana OX=3702 GN=At2g38480 PE=2 SV=2	[more]
Q5W6M3	6.9e-38	47.67	CASP-like protein 4B4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0344400 P...	[more]
C5WNF5	7.6e-37	42.08	CASP-like protein 4B1 OS=Sorghum bicolor OX=4558 GN=Sb01g038100 PE=2 SV=1	[more]
F2D276	6.4e-36	44.16	CASP-like protein 4B2 OS=Hordeum vulgare subsp. vulgare OX=112509 PE=2 SV=1	[more]

BLAST of Spg033371 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GHC2	1.6e-77	75.23	CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111454172 PE=3 SV=1	[more]
A0A6J1I265	1.3e-76	74.30	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111470217 PE=3 SV=1	[more]
A0A0A0L9S4	8.1e-74	74.24	CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G730790 PE=3 SV=1	[more]
A0A6J1DT05	8.1e-74	76.00	CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111024030 PE=3 SV=1	[more]
A0A5A7TQT0	2.9e-71	72.73	CASP-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G00...	[more]

BLAST of Spg033371 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38480.1	3.1e-41	50.00	Uncharacterised protein family (UPF0497)	[more]
AT2G36330.1	1.6e-18	34.57	Uncharacterised protein family (UPF0497)	[more]
AT5G62820.1	2.7e-13	32.08	Uncharacterised protein family (UPF0497)	[more]
AT4G11655.1	1.9e-11	27.78	Uncharacterised protein family (UPF0497)	[more]
AT5G40300.1	4.3e-11	29.93	Uncharacterised protein family (UPF0497)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006702	Casparian strip membrane protein domain	PFAM	PF04535	DUF588	coord: 53..179 e-value: 6.9E-26 score: 90.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..34
None	No IPR available	PANTHER	PTHR11615:SF144	CASP-LIKE PROTEIN 4B1	coord: 18..197
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 18..197

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg033371.1	Spg033371.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding