Spg033282 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg033282
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCytochrome P450
Locationscaffold5: 3649471 .. 3654156 (+)
RNA-Seq ExpressionSpg033282
SyntenySpg033282
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTTGAAATTGCAGGTTTTATTTTTGCCTCCATTCTGTGGGGTTTCTGGGTATGGCGCCGCCGTGGATCGGCGGAGGCGGAGCAGCTTCCGCCGGGGCCACCTCGTTGGCCGGTGGTCGGAAACTTGCCCCAACTCAGCTTCTCCGCTCACGAATCCTTCACCAAACTCGCCACCAAGTACGGCCCAATCATGACCCTCCATCTCGGGTCGATGAGCACGGTGGTCGTCTCGTCGAGCACGGCGGCGCGTGAGATGTTCAAAAACCACGACGCGCCGCTGGCAGGGCGCATGATTTACGAGGCAATGAAAGGGAACCACGGCACGGAAGGCTCCCTAATCACCTCCCAGTACGGGCCCCACTGGCGGATGCTGAGACGCCTCGCCACCACAGAGTTCTTCGTCACGCGCCGCCTCGACGCCATGCGACACATCCGCCGGCGCTGCGTGGACGCAATGATCCGCCACGTCGAGGTGGAAGCAGGCATCGGCGCGTGTGGGGACGAGCCGCCGATCGAGTTGGGGAAATTGTTCTTCATAATGAGCTTTAATCTGATCGGGAATCTGATATTTTCGAAAGATCTTGTGGAGCCGAATTCGAAGAGGGGGAAGGGATTTTACTACCATGCGAGACGGGTGATGGAGATGGCCGGAAAGCCGAATGTGGCGGACTTTTTGCCGGCGTTCCGGTGGGTGGATCCGCAGGGGATAAGGAGGAAGACGCAGTTTCATGTGAAGAAAGCGTTTGAAATTGCAGGGGAGTTTATAAGAGAGAGATTGAAGGAAAAAGAAAGTGATGAAAGAAAAGAAGAAGGAAGAAAGGATTATTTGGATGTGCTTTTGGAGTTTCGTGGCGATGGCGTCGAGGAGCCTTCATGTTTCTCTTCTTGGATCATCAACGTTATCGTCTTCGTAAGCTATTATTCATATTATCATTCTTTAATTGCTTATGCTTTTCTTTTCAATGGAGATTTTGGAGGGTGGTAGGAATTTCTTTCCTAAAATTTTTACACGTTCTTTTTTTTTTTTTTTTGAAAAAAAAAGTTTTTAAAAAAATCTTTCTTCTTCTCTTTATTTTCTTAAAGTTTAATGGGAACAATACTTGACTACGATACATGGGGCTGTCTTCCTACACACCCCGTTAAGATCTTGACAACTAAGAGAAGAAAAGGTAAAAAATGAGCAATTTATTTTCTGACGTTTTAATGGTAATGTGTATGGAGATCTCTACATATTGGTATGCAGCCACATATTTTCCATAAATTTTTACTATTTCTTTTAGTTCAATAATATGTGGGGTGAGCTCTTCAACTACCAGCCAAAGCGTTGACAATTCCCTAAATGCTAAAGATAGCTATACAATCGTCGACGTTTATCTCCTGACGGTACTCTCGACGATCTTAAAACCGTCGGTGAAACCGACGATTTACACCGTCGGGAAAGTCCAAAACTCTTGTAATGGAGGGATAGAAAATTCTTGGTTGCATACCCATATGCAAGAATCTCACTACACACCACCATTAAAACGTTGATAATTAGGAGAGATAAAATAAATTTCTCACTGTCTATCTTTCTCTCTTTAGTTGCAAGAATCTCATGTCTGTCGTTATGTAACCAAGTATTGTTCGTGAGGATTCAATCCTCTAATCTAATAGTCGATGAAGTATATATTGCTAAAGTGGTCAAGAAAAGTATTATCCTTCTTGTGGTATAAAAATAACATTAGTGAAAATAAGCTAATAAGAAACATGTAAGGATAGGAGATCAAACCATGTTGGCAGTTTTTACTTGTTAATTATGTTTAGAATGATAGTACGACTTGTTATGTGTAGGCTAGTAATGTTTATGTGATTAGCCACATAAAATTTGCATGATACTTCCCCCAACGTGCTCATCAATAATTTATCTTTTTATGGGTCTTCTAAAATGAAACGGAAAAACCTCTTTTCTATTTTATTTTCTCTAAATTCTTTCGTTTTTATTGGTAAAAAAATTTAGCGGTGGACAATAGAAACTCTTACCATCCAATCAATAGGATAATTATTATTCATAAATTTTGGTATAAATCTCATTTCCAATCTTCAATATTTTTAAGATTTTTTCTTTTTATTTTTCCTCCTTAACTTTTAGAAAAATAAATTTTGTATTTAGACTAGTGAAAATCATATCGAAAATTGACAATGAGAGATGGCGTATCTCACGTGATATGTTTTAGATATCATATAATACCTATGCTGTTTTTACAAAAATAAAAATATATATTATCTTATTGTTTATCATTTAAATGATTGATTTATTAAATTGTTGAAACATATCAGATTGTGGATCAAGATTAAAGAATCTCATTTAGGTTTTGTTTGTATTATTTTATAATTTTTTGAAAGATAATGTATTGACGATTATTTTATACACCATGCTTTTTTTAGTTCATAAATCATACTATTGTATTTTAGTAACTTGGAATATTATTGCAACAATCTTAAAATTATACTTTTATCTATTTATTTTCTTCAGAATTTTAGTCTTTCAATTATTTTTTATTTATTCATTTGGTTTTTTTTTTTTTGAAAAGATTTATTCATTTGGTTCTTAAACATTTTTAAATATTCTATTTCAATATCTAAACGTTTAATGTTTTGTTTTAGTCCTTACACTAAATAAATAATTTATATTTTTTCTCTATTAGTTTTTATTTAATCATTTAATAAAAATTTTAATCTATTTAGAACTTTAATAACCTAATCTATTAGATTATATGCAAATATGTTTTTGTTCATTTACTTGAACAACAAAATTATATTTCTATCAATTAAATACTTTATAAATAATAATAATAATATTAAAAATAATTTTAAAAAAAAAACTTATCACTCTAACTAGAGTGATTGATTTTAGAAAGAGAAACAAAAAAGAAAAAAAAAAAAGTCTAGACAAGAATGTTATTGTACCTTCGAAACCTCAATTTTAATAGCACTTTTTTATGATCACAATATCAACACAACATTACACTCAATAAATTTCTTGAAATGTTTTTGCAACTTTTATTTTAAAAGGTTTTGGTTGTATATAATAAATTTTTGTTAGAATCTTACAAATAAGAAATCAAAATGGTCTTTTTTTTTTTTTTAGTATATAAACCAAAACAAGTGTTTTGAAATTATAGAAACCAAAATGAATAAATGTTTGAAAACCAAAGGGAGACCAACATAGATAAATGTTTCTTTTAGGGCTTTATACAATTTGGTCATTACTTCAAAAGTTTAAATTTAGTTCGTTAGTTCAATTATCTTTTCATATCAATCAATGTTAAAGTTGACCGATGATAATTTCACGTGGGACAAGATACTTAGTGAGTTGGTAGAAATTTATAAAACAAATTATACCATTTAAGAAAATAAAAGTCCAAATTTGTACCAATTTCTTATATATTTAAAAGAGAATTTAGAAACTTCCCCTCTCTTAAATTCCCAAAATTCCACCTTTGAAATTCTGCAACATATTAAATATCATGTTTATGGTAAATTTCTCGTCAACGTTTTTTTTCCTTTTTGATTAAATTGAAATTTTTAAATATTTTTCATCAACGTTTTTTCTTTTTGACAAATTTGTGGGGGTGGGAATTCGAACCACAGATCTTTTGGTTCTTAGTACAACTTTATGTCAGTTGAACTATGTTCATGTTGGCATATTTCTCATCAGTATTGTAATGAACTAAATTGAAAATTTTTAAATAATTAAAAAGTAGATATATTAAAAAAAAAAAAAAAACACCCTCAAACATTTAAAGTTTAACCTATATATAACCTTAGTCCAGCTTAGGTTTTATTTTTATTTTTTATTATTATTATTATTTTTTTTTTTTTTTTTTTTGAGTTGAGGTATTATATCTTCATTCTCGAGCATTGGTTGAAAGAAATAATAATGATAATAATTTTTTTAGAGAGAAATAATAATAATAATCATGAAAAAAAATAATGATAATAATAATACACCTAAATATACGATACTTCGCAAAAATGTTATTTTGTTTTCTTTTTTTTTTTTTTTCAGGAGATGTTCACGGCGGGGACGGACACGACCACCAGCACACTAGAATGGGCAATGACGGAGCTCCTCCACAACCCGGCGGCGTTGCAGAAACTTCAGGCAGAGCTCCGATCTACCATCCCGCCGGACGGCAACCTCGAAGAAACCCATCTCCAAAACCTCCCGTATCTCGACGCCGTCGTCAAAGAAACCCTCCGCCTCCACCCGCCGTTGCCGTTCCTCTTCCCGAGAATGGCCATGAAGCCCTGCAAAATTCTCGGCTACCGAATTCCGGCGGAGACCCAAGTTTTGGTCAACGTTTGGGCCATCGGGCGAGACCCCAAAAACTGGAAAGACGCTACGGTTTTCTCGCCGGAGAGATTCTTGGAGCCCGGCGCCGGGGCCGGGAAGGATTTTAAAGGTCAGCACTTTGACTTCCTTCCTTTTGGGTCCGGCCGGCGCATGTGCCCGGCGGTGCCGCTGGCGTCGAGGGTTCTTCCGGTGGCTTTGGGTTCGTTGATTCTGTCGTTTGATTGGGCCCTGCCGGACGGAATGTTGCCGGAGGAGATGGACATGTCGGAGCAAATGGGGATTACTCTTAGAAAGAAAGTTCCTTTGAAGGCCATTCCGACACCTTATAAACGACGTCGTTTCTGA

mRNA sequence

ATGGAGTTTGAAATTGCAGGTTTTATTTTTGCCTCCATTCTGTGGGGTTTCTGGGTATGGCGCCGCCGTGGATCGGCGGAGGCGGAGCAGCTTCCGCCGGGGCCACCTCGTTGGCCGGTGGTCGGAAACTTGCCCCAACTCAGCTTCTCCGCTCACGAATCCTTCACCAAACTCGCCACCAAGTACGGCCCAATCATGACCCTCCATCTCGGGTCGATGAGCACGGTGGTCGTCTCGTCGAGCACGGCGGCGCGTGAGATGTTCAAAAACCACGACGCGCCGCTGGCAGGGCGCATGATTTACGAGGCAATGAAAGGGAACCACGGCACGGAAGGCTCCCTAATCACCTCCCAGTACGGGCCCCACTGGCGGATGCTGAGACGCCTCGCCACCACAGAGTTCTTCGTCACGCGCCGCCTCGACGCCATGCGACACATCCGCCGGCGCTGCGTGGACGCAATGATCCGCCACGTCGAGGTGGAAGCAGGCATCGGCGCGTGTGGGGACGAGCCGCCGATCGAGTTGGGGAAATTGTTCTTCATAATGAGCTTTAATCTGATCGGGAATCTGATATTTTCGAAAGATCTTGTGGAGCCGAATTCGAAGAGGGGGAAGGGATTTTACTACCATGCGAGACGGGTGATGGAGATGGCCGGAAAGCCGAATGTGGCGGACTTTTTGCCGGCGTTCCGGTGGGTGGATCCGCAGGGGATAAGGAGGAAGACGCAGTTTCATGTGAAGAAAGCGTTTGAAATTGCAGGGGAGTTTATAAGAGAGAGATTGAAGGAAAAAGAAAGTGATGAAAGAAAAGAAGAAGGAAGAAAGGATTATTTGGATGTGCTTTTGGAGTTTCGTGGCGATGGCGTCGAGGAGCCTTCATGTTTCTCTTCTTGGATCATCAACGTTATCGTCTTCGAGATGTTCACGGCGGGGACGGACACGACCACCAGCACACTAGAATGGGCAATGACGGAGCTCCTCCACAACCCGGCGGCGTTGCAGAAACTTCAGGCAGAGCTCCGATCTACCATCCCGCCGGACGGCAACCTCGAAGAAACCCATCTCCAAAACCTCCCGTATCTCGACGCCGTCGTCAAAGAAACCCTCCGCCTCCACCCGCCGTTGCCGTTCCTCTTCCCGAGAATGGCCATGAAGCCCTGCAAAATTCTCGGCTACCGAATTCCGGCGGAGACCCAAGTTTTGGTCAACGTTTGGGCCATCGGGCGAGACCCCAAAAACTGGAAAGACGCTACGGTTTTCTCGCCGGAGAGATTCTTGGAGCCCGGCGCCGGGGCCGGGAAGGATTTTAAAGGTCAGCACTTTGACTTCCTTCCTTTTGGGTCCGGCCGGCGCATGTGCCCGGCGGTGCCGCTGGCGTCGAGGGTTCTTCCGGTGGCTTTGGGTTCGTTGATTCTGTCGTTTGATTGGGCCCTGCCGGACGGAATGTTGCCGGAGGAGATGGACATGTCGGAGCAAATGGGGATTACTCTTAGAAAGAAAGTTCCTTTGAAGGCCATTCCGACACCTTATAAACGACGTCGTTTCTGA

Coding sequence (CDS)

ATGGAGTTTGAAATTGCAGGTTTTATTTTTGCCTCCATTCTGTGGGGTTTCTGGGTATGGCGCCGCCGTGGATCGGCGGAGGCGGAGCAGCTTCCGCCGGGGCCACCTCGTTGGCCGGTGGTCGGAAACTTGCCCCAACTCAGCTTCTCCGCTCACGAATCCTTCACCAAACTCGCCACCAAGTACGGCCCAATCATGACCCTCCATCTCGGGTCGATGAGCACGGTGGTCGTCTCGTCGAGCACGGCGGCGCGTGAGATGTTCAAAAACCACGACGCGCCGCTGGCAGGGCGCATGATTTACGAGGCAATGAAAGGGAACCACGGCACGGAAGGCTCCCTAATCACCTCCCAGTACGGGCCCCACTGGCGGATGCTGAGACGCCTCGCCACCACAGAGTTCTTCGTCACGCGCCGCCTCGACGCCATGCGACACATCCGCCGGCGCTGCGTGGACGCAATGATCCGCCACGTCGAGGTGGAAGCAGGCATCGGCGCGTGTGGGGACGAGCCGCCGATCGAGTTGGGGAAATTGTTCTTCATAATGAGCTTTAATCTGATCGGGAATCTGATATTTTCGAAAGATCTTGTGGAGCCGAATTCGAAGAGGGGGAAGGGATTTTACTACCATGCGAGACGGGTGATGGAGATGGCCGGAAAGCCGAATGTGGCGGACTTTTTGCCGGCGTTCCGGTGGGTGGATCCGCAGGGGATAAGGAGGAAGACGCAGTTTCATGTGAAGAAAGCGTTTGAAATTGCAGGGGAGTTTATAAGAGAGAGATTGAAGGAAAAAGAAAGTGATGAAAGAAAAGAAGAAGGAAGAAAGGATTATTTGGATGTGCTTTTGGAGTTTCGTGGCGATGGCGTCGAGGAGCCTTCATGTTTCTCTTCTTGGATCATCAACGTTATCGTCTTCGAGATGTTCACGGCGGGGACGGACACGACCACCAGCACACTAGAATGGGCAATGACGGAGCTCCTCCACAACCCGGCGGCGTTGCAGAAACTTCAGGCAGAGCTCCGATCTACCATCCCGCCGGACGGCAACCTCGAAGAAACCCATCTCCAAAACCTCCCGTATCTCGACGCCGTCGTCAAAGAAACCCTCCGCCTCCACCCGCCGTTGCCGTTCCTCTTCCCGAGAATGGCCATGAAGCCCTGCAAAATTCTCGGCTACCGAATTCCGGCGGAGACCCAAGTTTTGGTCAACGTTTGGGCCATCGGGCGAGACCCCAAAAACTGGAAAGACGCTACGGTTTTCTCGCCGGAGAGATTCTTGGAGCCCGGCGCCGGGGCCGGGAAGGATTTTAAAGGTCAGCACTTTGACTTCCTTCCTTTTGGGTCCGGCCGGCGCATGTGCCCGGCGGTGCCGCTGGCGTCGAGGGTTCTTCCGGTGGCTTTGGGTTCGTTGATTCTGTCGTTTGATTGGGCCCTGCCGGACGGAATGTTGCCGGAGGAGATGGACATGTCGGAGCAAATGGGGATTACTCTTAGAAAGAAAGTTCCTTTGAAGGCCATTCCGACACCTTATAAACGACGTCGTTTCTGA

Protein sequence

MEFEIAGFIFASILWGFWVWRRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLFFIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRERLKEKESDERKEEGRKDYLDVLLEFRGDGVEEPSCFSSWIINVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATVFSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALPDGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRRRF
Homology
BLAST of Spg033282 vs. NCBI nr
Match: XP_022935159.1 (cytochrome P450 76A1 [Cucurbita moschata])

HSP 1 Score: 873.2 bits (2255), Expect = 1.1e-249
Identity = 436/514 (84.82%), Postives = 457/514 (88.91%), Query Frame = 0

Query: 1   MEFEIAGFIFASILWGFWVWRRRGSAE-AEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I  S LWG WVWRRRG+A   EQLPPGP RWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAALILVSFLWGLWVWRRRGAAALGEQLPPGPTRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDAPL+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDAPLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAMI++VE E    A    PP+++GK F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMIQYVEDEIKGSAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV PNSKRGK FYYH RRVME AG PNVADFL  FRW DPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMETAGTPNVADFLRVFRWFDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESDERKEEGRKDYLDVLLEFRGDGVEEPSCFSSWI 300
           R TQFHVK+AFEIAGEFI+ER  EK   E+KEE +KDYLDVLLEFRGDGV EPS FSSWI
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSMEK---EKKEERKKDYLDVLLEFRGDGVVEPSRFSSWI 300

Query: 301 INVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLP 360
           INVIV EMFTAGTDTTTS+LEWAM ELL NPAA+QKLQAELRSTIPPDG LEETHLQNLP
Sbjct: 301 INVIVLEMFTAGTDTTTSSLEWAMAELLRNPAAMQKLQAELRSTIPPDGKLEETHLQNLP 360

Query: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATV 420
           YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGY IPAETQVLVNVWAIGRDPKNWKDA V
Sbjct: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYWIPAETQVLVNVWAIGRDPKNWKDALV 420

Query: 421 FSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALP 480
           FSPERFLEP     KDFKGQHFDFLPFGSGRRMCPAVPLASRVLP+ALGSLIL FDWALP
Sbjct: 421 FSPERFLEP--TGSKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPMALGSLILRFDWALP 480

Query: 481 DGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRR 514
           DG+LPEEMDMSEQMGITL+KKVPLKAIP P+KRR
Sbjct: 481 DGLLPEEMDMSEQMGITLKKKVPLKAIPIPHKRR 509

BLAST of Spg033282 vs. NCBI nr
Match: XP_022982917.1 (cytochrome P450 76A2 [Cucurbita maxima])

HSP 1 Score: 872.1 bits (2252), Expect = 2.4e-249
Identity = 436/514 (84.82%), Postives = 458/514 (89.11%), Query Frame = 0

Query: 1   MEFEIAGFIFASILWGFWVWRRRGSAE-AEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I  S LWG WVWRRRG+A   EQLPPGPPRWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAALILVSFLWGLWVWRRRGAAALGEQLPPGPPRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDA L+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDASLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAMI++VE E    A    PP+++GK F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMIQYVEDEIKGSAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV PNSKRGK FYYH RRVME+AG PNVADFL  FRWVDPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMEIAGTPNVADFLRVFRWVDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESDERKEEGRKDYLDVLLEFRGDGVEEPSCFSSWI 300
           R TQFHVK+AFEIAGEFI+ER  EK   E+KEE +KDYLDVLLEFRGDGV EPS FSSWI
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSTEK---EKKEERKKDYLDVLLEFRGDGVVEPSRFSSWI 300

Query: 301 INVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLP 360
           INVIV EMFTAGTDTTTS+LEWAM ELL NPAA+QKLQAELRSTI PDG LEETHLQNLP
Sbjct: 301 INVIVLEMFTAGTDTTTSSLEWAMAELLRNPAAMQKLQAELRSTILPDGKLEETHLQNLP 360

Query: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATV 420
           YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGY IPAETQVLVNVWAIGRDPKNWKDA V
Sbjct: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYWIPAETQVLVNVWAIGRDPKNWKDALV 420

Query: 421 FSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALP 480
           FSPERFLEP     KDFKGQHFDFLPFGSGRRMCPAVPLASRVLP+ALGSLIL FDWALP
Sbjct: 421 FSPERFLEP--TGSKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPMALGSLILRFDWALP 480

Query: 481 DGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRR 514
           DG+LPEEMDMSEQMGITL+KKVPLKAIP P+KRR
Sbjct: 481 DGLLPEEMDMSEQMGITLKKKVPLKAIPIPHKRR 509

BLAST of Spg033282 vs. NCBI nr
Match: XP_004138005.1 (iridoid oxidase [Cucumis sativus] >KGN59047.1 hypothetical protein Csa_001475 [Cucumis sativus])

HSP 1 Score: 872.1 bits (2252), Expect = 2.4e-249
Identity = 430/525 (81.90%), Postives = 465/525 (88.57%), Query Frame = 0

Query: 1   MEFE-IAGFIFASILWGFWVWRRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME E IAGFI    LWG W+WR RG+    QLPPGPP WPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MELEIIAGFILLLFLWGLWLWRCRGAGADRQLPPGPPCWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFK HD PL+GRMIYEAMKGNHGT+GSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTVAREMFKTHDVPLSGRMIYEAMKGNHGTQGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLF 180
           GPHWRMLRRLAT+EFFV R+L++M+H+RR C+DAMIRH  VE G   CG+ P +++GK  
Sbjct: 121 GPHWRMLRRLATSEFFVARQLESMKHVRRHCIDAMIRH--VEEGCDPCGNAPALDVGKFV 180

Query: 181 FIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           F+M FNLIGNLIFSKDL+E NSKRGK FYYH R+VMEMAGKPNVADFLPA RW+DPQGIR
Sbjct: 181 FVMGFNLIGNLIFSKDLLEANSKRGKEFYYHTRKVMEMAGKPNVADFLPALRWLDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERL--KEK------ESDERKEE-GRKDYLDVLLEFRGDGVE 300
           RKTQFHV KAFEIAGEFI+ERL  +EK      + DE+KEE G+KDYLDVLLEFRGDGVE
Sbjct: 241 RKTQFHVNKAFEIAGEFIKERLLIREKRDIINDDDDEKKEESGKKDYLDVLLEFRGDGVE 300

Query: 301 EPSCFSSWIINVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNL 360
           EPS FSSWIINVIV EMFTAGTDTTTSTLEWAM ELLHNPA+LQKLQAELR+TI P+ NL
Sbjct: 301 EPSRFSSWIINVIVLEMFTAGTDTTTSTLEWAMAELLHNPASLQKLQAELRATIAPNDNL 360

Query: 361 EETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRD 420
           EETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPC ILGYRIPAETQVLVN WAIGRD
Sbjct: 361 EETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCNILGYRIPAETQVLVNFWAIGRD 420

Query: 421 PKNWKDATVFSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSL 480
           PKNWKDA VFSPERF +P +G+ KDFKGQHFDF+PFGSGRRMCPAVPLASRVLP+ALGSL
Sbjct: 421 PKNWKDALVFSPERFFDPSSGS-KDFKGQHFDFIPFGSGRRMCPAVPLASRVLPMALGSL 480

Query: 481 ILSFDWALPDGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRRRF 516
           ILSFDWALPDG+ P+EMDMSEQMGITLRKKVPLKAIPTP+KRRRF
Sbjct: 481 ILSFDWALPDGVSPKEMDMSEQMGITLRKKVPLKAIPTPHKRRRF 522

BLAST of Spg033282 vs. NCBI nr
Match: XP_023528102.1 (cytochrome P450 76A2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 870.9 bits (2249), Expect = 5.4e-249
Identity = 433/514 (84.24%), Postives = 457/514 (88.91%), Query Frame = 0

Query: 1   MEFEIAGFIFASILWGFWVWRRRGSAE-AEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I  S LWG WVWRRRG+A   EQLPPGPPRWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAAVILVSFLWGLWVWRRRGAAALGEQLPPGPPRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDAPL+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDAPLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAM+++VE E    A    PP+++GK F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMVQYVEDEIKGSAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV PNSKRGK FYYH RRVME AG PNVADFL  FRW DPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMETAGTPNVADFLRVFRWFDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESDERKEEGRKDYLDVLLEFRGDGVEEPSCFSSWI 300
           R TQFHVK+AFEIAGEFI+ER  EK   E+KEE +KDYLDVLLEF GDGV EPS FSSWI
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSMEK---EKKEERKKDYLDVLLEFHGDGVVEPSRFSSWI 300

Query: 301 INVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLP 360
           INVIV EMFTAGTDTTTS+LEWAM ELL NPAA+QKLQAELRSTIPPDG LEETHLQNLP
Sbjct: 301 INVIVLEMFTAGTDTTTSSLEWAMAELLRNPAAMQKLQAELRSTIPPDGKLEETHLQNLP 360

Query: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATV 420
           YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGY IPAETQVLVNVWAIGRDPKNWKDA V
Sbjct: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYWIPAETQVLVNVWAIGRDPKNWKDALV 420

Query: 421 FSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALP 480
           FSPERFLEP     KDF+GQHFDFLPFGSGRRMCPAVPLASRVLP+ALGSLIL FDWALP
Sbjct: 421 FSPERFLEP--TGSKDFRGQHFDFLPFGSGRRMCPAVPLASRVLPMALGSLILRFDWALP 480

Query: 481 DGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRR 514
           +G+LPEEMDMSEQMGITL+KKVPLKAIP P+KRR
Sbjct: 481 EGLLPEEMDMSEQMGITLKKKVPLKAIPIPHKRR 509

BLAST of Spg033282 vs. NCBI nr
Match: XP_038905939.1 (iridoid oxidase [Benincasa hispida])

HSP 1 Score: 855.9 bits (2210), Expect = 1.8e-244
Identity = 424/523 (81.07%), Postives = 457/523 (87.38%), Query Frame = 0

Query: 1   MEFEIAGFIFASILWGFWVWRRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLAT 60
           ME +IAG I    LWG WVWR RG+   EQLPPGPPRWPVVGNL QLSFSAHESFTKLA 
Sbjct: 1   MEIQIAGLILLLFLWGLWVWRHRGAVADEQLPPGPPRWPVVGNLLQLSFSAHESFTKLAA 60

Query: 61  KYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYG 120
           KYGPIMTL LGSM TVVVSSS AAREMFK HDAPL+GRMIYEAMKG HGTEGSLITSQYG
Sbjct: 61  KYGPIMTLQLGSMKTVVVSSSAAAREMFKTHDAPLSGRMIYEAMKGEHGTEGSLITSQYG 120

Query: 121 PHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHV--EVEAGIGACGDEPPIELGKL 180
           PHWRMLRRLAT+EFFVTR+L+AM+H+RRRC+D MIRH+  E+E G+ A    PP+++GK 
Sbjct: 121 PHWRMLRRLATSEFFVTRQLEAMKHVRRRCIDTMIRHIEEEIEGGVHA----PPLDVGKF 180

Query: 181 FFIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGI 240
            FIMSFNLIGNLIFSKDL+E NSKRGK FYYH R+VMEMAGKPNVADFLPA RW+DPQGI
Sbjct: 181 MFIMSFNLIGNLIFSKDLLEANSKRGKEFYYHTRKVMEMAGKPNVADFLPALRWMDPQGI 240

Query: 241 RRKTQFHVKKAFEIAGEFIRERLKEKE------SDERKEEGRKDYLDVLLEFRGDGVEEP 300
           R+KTQFHV KAFEIAGEFI+ERL  +E      SDE+KEE +KDYLDVLLEFRGDGV EP
Sbjct: 241 RKKTQFHVNKAFEIAGEFIKERLLLREKGNIIISDEKKEERKKDYLDVLLEFRGDGVVEP 300

Query: 301 SCFSSWIINVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEE 360
           S FSSWIINVIVFEMFTAGTDTTTST   AM ELLHNPAALQKLQAELR+TIPP+   ++
Sbjct: 301 SQFSSWIINVIVFEMFTAGTDTTTSTHRMAMAELLHNPAALQKLQAELRATIPPNATSKK 360

Query: 361 THLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPK 420
           THLQNLPYLDAVVKETLRLHPPLPFLFPRMAMK CKI GYRIPAETQVLVN WAIGRDPK
Sbjct: 361 THLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKHCKIFGYRIPAETQVLVNFWAIGRDPK 420

Query: 421 NWKDATVFSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLIL 480
           NWKDA VFSPERFL   +   KDFKGQHFDF+PFGSGRRMCPAVPLASRVLP+ALGSLIL
Sbjct: 421 NWKDALVFSPERFLV--STDSKDFKGQHFDFIPFGSGRRMCPAVPLASRVLPMALGSLIL 480

Query: 481 SFDWALPDGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRRRF 516
           SFDWALP+GM PEEMDMS QMGITLR KVPLKA+PT +KRRRF
Sbjct: 481 SFDWALPEGMSPEEMDMSAQMGITLRMKVPLKAVPTRHKRRRF 517

BLAST of Spg033282 vs. ExPASy Swiss-Prot
Match: W8JIS5 (Iridoid oxidase OS=Catharanthus roseus OX=4058 GN=IO PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 6.6e-125
Identity = 216/503 (42.94%), Postives = 335/503 (66.60%), Query Frame = 0

Query: 6   AGFIFASILWGFWVWRRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPI 65
           AGF+   I+   +V  R GS++ +  PPGPP WP+ GN+  L    H++  KL +KYGPI
Sbjct: 18  AGFLLLLII---FVKSRTGSSKRK--PPGPPGWPIFGNMFDLGDLPHQTLYKLKSKYGPI 77

Query: 66  MTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRM 125
           + L LGS++T+VV ++ +A E+FK HD P   R + + +   +  +GSL  + YG HWR+
Sbjct: 78  VWLQLGSINTMVVQNAVSAAELFKKHDVPFCDRKVPDTLTAFNFNQGSLGMNTYGGHWRV 137

Query: 126 LRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEA-GIGACGDEPPIELGKLFFIMSF 185
           LRRL + EF V +R++    +RRR  D M+R +E ++    A G    ++L +  F+M+F
Sbjct: 138 LRRLCSMEFLVNKRMNETTDLRRRIEDNMVRWIEEDSLASKAQGGTGAVQLSRFLFLMAF 197

Query: 186 NLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQF 245
           NL+GNL+ S+DL++     G+ F+     ++E+AG PN+ADFLP  + +DP G++++   
Sbjct: 198 NLVGNLMLSRDLMDNKDPEGREFFDCMNEILELAGTPNIADFLPLLKKLDPLGMKKRMVD 257

Query: 246 HVKKAFEIAGEFIRERLKEKESDERKEEGRKDYLDVLLEFRGDGVEEPSCFSSWIINVIV 305
           ++ +  +I+ +F++ERL  +++ +  E  +KD+LDV+LE++GDG + P  F+   +N+++
Sbjct: 258 NMSRTMKISSKFVQERLDNRKAGKINE--KKDFLDVMLEYQGDGKDGPDKFTEQHVNIVI 317

Query: 306 FEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLPYLDAV 365
            EMF AG++TT+ ++EW  TELL NP A +K++ E+   +  +  +EET ++NLPYL AV
Sbjct: 318 MEMFFAGSETTSISIEWGFTELLRNPHAFKKVREEIDRVVGVNRMVEETDMENLPYLQAV 377

Query: 366 VKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATVFSPER 425
           VKETLRLHP LP L PR  M+  + +GY IP  TQV VN WAIGRDP+ W+D   F PER
Sbjct: 378 VKETLRLHPALPMLLPRNTMEDTEYMGYLIPKGTQVFVNAWAIGRDPEYWQDPLSFKPER 437

Query: 426 FLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALPDGMLP 485
           F+     +  ++KGQHF+ +PFGSGRR+C   PLA RV+ + L +L+ +FDW L  G+ P
Sbjct: 438 FI----NSSVEYKGQHFELIPFGSGRRICVGFPLAHRVVHLTLATLVQAFDWDLGAGVKP 497

Query: 486 EEMDMSEQMGITLRKKVPLKAIP 508
           +++D+ E++G+TLRKK PL  IP
Sbjct: 498 QDIDLEERLGLTLRKKNPLNVIP 509

BLAST of Spg033282 vs. ExPASy Swiss-Prot
Match: P37122 (Cytochrome P450 76A2 OS=Solanum melongena OX=4111 GN=CYP76A2 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.3e-120
Identity = 221/511 (43.25%), Postives = 325/511 (63.60%), Query Frame = 0

Query: 1   MEFEIAGFIFASIL----WGFWVWRRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFT 60
           ME+E +   F++I+    +  +  ++  +  + + PPGPP  P+ GN+ +L    ++   
Sbjct: 1   MEWEWSYVFFSAIIILPAFILFFSQKNTTKSSYKFPPGPPGLPIFGNMFELGTEPYKKMA 60

Query: 61  KLATKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLIT 120
            L  KYGP++ L LGS  T+VV ++ A+ E+FKNHD   A R+I +  + +   +GSL  
Sbjct: 61  VLRQKYGPVLWLKLGSTYTMVVQTAQASEELFKNHDISFANRVIPDVNQAHSYYQGSLAI 120

Query: 121 SQYGPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELG 180
           + YGP WR  RR+ T E FV +++     +RR+CVD M++ +E EA     G    IE+ 
Sbjct: 121 APYGPFWRFQRRICTIEMFVHKKISETEPVRRKCVDNMLKWIEKEANSAEKGS--GIEVT 180

Query: 181 KLFFIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQ 240
           +  F+ SFN++GNLI SKDL +  S+    F+   +R+ E +G  NV+D  P  +  D Q
Sbjct: 181 RFVFLASFNMLGNLILSKDLADLESEEASEFFIAMKRINEWSGIANVSDIFPFLKKFDLQ 240

Query: 241 GIRRKTQFHVKKAFEIAGEFIRERLKEKESDERKEEGRKDYLDVLLEFRGDGVEEPSCFS 300
            +R+K    + KA EI   F++ER  E+E  +  E+G KD+LDVLLEF+G G +EP+  S
Sbjct: 241 SLRKKMARDMGKAVEIMSMFLKER--EEERKKGTEKG-KDFLDVLLEFQGTGKDEPAKLS 300

Query: 301 SWIINVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQ 360
              I + V EMF AGT+TT+S++EWA+TELL +P A+ K++ E+   I P+   E++ ++
Sbjct: 301 EHEIKIFVLEMFLAGTETTSSSVEWALTELLRHPEAMAKVKTEISQAIEPNRKFEDSDIE 360

Query: 361 NLPYLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKD 420
           NLPY+ AV+KE+LRLHPPLPFL PR  ++  K +GY +P +TQVLVN WAIGRDP+ W D
Sbjct: 361 NLPYMQAVLKESLRLHPPLPFLIPRETIQDTKFMGYDVPKDTQVLVNAWAIGRDPECWDD 420

Query: 421 ATVFSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDW 480
              F PERFL    G+  D KGQH+  +PFG+GRRMC  +PL  R++  ALGSL+  F+W
Sbjct: 421 PMSFKPERFL----GSKIDVKGQHYGLIPFGAGRRMCVGLPLGHRMMHFALGSLLREFEW 480

Query: 481 ALPDGMLPEEMDMSEQMGITLRKKVPLKAIP 508
            LPDG+ P+ ++M   MG+T RK+  LK IP
Sbjct: 481 ELPDGVSPKSINMDGSMGVTARKRDSLKVIP 502

BLAST of Spg033282 vs. ExPASy Swiss-Prot
Match: P37121 (Cytochrome P450 76A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP76A1 PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 5.8e-113
Identity = 213/469 (45.42%), Postives = 303/469 (64.61%), Query Frame = 0

Query: 42  GNLPQLSFSA-HESFTKLATKYGPIMTLHLG-SMSTVVVSSSTAAREMFKNHDAPLAGRM 101
           GN+  L+ SA ++    L  KYGPI+ L +G SM+T+V+ ++ +A E+F+NHD   + R 
Sbjct: 2   GNMFDLAGSAPYKKIACLKEKYGPILWLKIGSSMNTMVIQTANSASELFRNHDVSFSDRP 61

Query: 102 IYEAMKGNHGTEGSLITSQYGPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVE 161
           I +    ++  +GS+  + YG +WR  RR+ T E FV +R++   +IR+  VD M+R  E
Sbjct: 62  IVDVNLAHNYYKGSMALAPYGNYWRFSRRICTVEMFVHKRINETTNIRQESVDKMLRLDE 121

Query: 162 VEAGIGACGDEPPIELGKLFFIMSFNLIGNLIFSKDLV-EPNSKRGKGFYYHARRVMEMA 221
            +A     G E  IE+ +  F+ SFN++GN+IFSKDLV +P SK+G  F+     +ME A
Sbjct: 122 EKASSSGGGGE-GIEVTRYMFLASFNMVGNMIFSKDLVTDPESKQGSEFFNAMIGIMEWA 181

Query: 222 GKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRERLKEKESDERKEEGRKDYL 281
           G PN++D  P  +  D QG+R+K +  + K  EI  +FI ER++E++  E K    KD L
Sbjct: 182 GVPNISDIFPCLKMFDVQGLRKKMERDMGKGKEITKKFIEERIEERKKGE-KNRSIKDLL 241

Query: 282 DVLLEFRGDGVEEPSCFSSWIINVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQA 341
           DVL++F G G +EP   S   I VI+ EMF AGT+TT+S++EWA+TELL +P A+ K++ 
Sbjct: 242 DVLIDFEGSGKDEPDKLSEDEITVIILEMFLAGTETTSSSVEWALTELLRHPQAMAKVKL 301

Query: 342 ELRSTIPPDGNLEETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAET 401
           E+   I P+   EE  + +LPY+ AV+KE LRLHPPLP L PR A++  K +GY IP  T
Sbjct: 302 EILQVIGPNKKFEECDIDSLPYMQAVLKEQLRLHPPLPLLIPRKAIQDTKFMGYDIPKGT 361

Query: 402 QVLVNVWAIGRDPKNWKDATVFSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPL 461
           QVLVN WAIGRDP+ W +   F PERFLE    +  D KGQ+++ +PFG+GRRMC  +PL
Sbjct: 362 QVLVNAWAIGRDPEYWDNPFEFKPERFLE----SKVDVKGQNYELIPFGAGRRMCVGLPL 421

Query: 462 ASRVLPVALGSLILSFDWALPDGMLPEEMDMSEQMGITLRKKVPLKAIP 508
             R++    GSL+  FDW LP  + P+ ++M E MGIT RKK PLK IP
Sbjct: 422 GHRMMHFTFGSLLHEFDWELPHNVSPKSINMEESMGITARKKQPLKVIP 464

BLAST of Spg033282 vs. ExPASy Swiss-Prot
Match: Q8VWZ7 (Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 3.6e-107
Identity = 203/484 (41.94%), Postives = 289/484 (59.71%), Query Frame = 0

Query: 25  SAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPIMTLHLGSMSTVVVSSSTAA 84
           S   + LPPGP   P +G+L  L    H+S  KL+ K+GPIM+L LG ++T+V+SSST A
Sbjct: 24  SRRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKKHGPIMSLKLGQITTIVISSSTMA 83

Query: 85  REMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRMLRRLATTEFFVTRRLDAMR 144
           +E+ +  D   + R +  A+  ++  + S++       WR LR++  +  F   RLDA +
Sbjct: 84  KEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVASRWRSLRKVLNSNIFSGNRLDANQ 143

Query: 145 HIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLFFIMSFNLIGNLIFSKDLVEPNSKRG 204
           H+R R V  +I +    +  G       +++G+  F  S NL+ NLIFSKDL +P S   
Sbjct: 144 HLRTRKVQELIAYCRKNSQSGEA-----VDVGRAAFRTSLNLLSNLIFSKDLTDPYSDSA 203

Query: 205 KGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRERLKEK 264
           K F      +M  AGKPN+ DF P    VDPQGIR +   H  +  ++ G  + ERL+++
Sbjct: 204 KEFKDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEVLKLFGGLVNERLEQR 263

Query: 265 ESDERKEEGRKDYLDVLLEFRGDGVEEPSCFSSWIINVIVFEMFTAGTDTTTSTLEWAMT 324
            S   K     D LDVLL       E P       I  +  ++F AGTDTT+STLEWAM+
Sbjct: 264 RSKGEK----NDVLDVLLT---TSQESPEEIDRTHIERMCLDLFVAGTDTTSSTLEWAMS 323

Query: 325 ELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAM 384
           E+L NP  ++K Q EL   I     +EE+ +  LPYL  V+KETLR+HPP+PFL PR   
Sbjct: 324 EMLKNPDKMKKTQDELAQVIGRGKTIEESDINRLPYLRCVMKETLRIHPPVPFLIPRKVE 383

Query: 385 KPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATVFSPERFLEPGAGAGKDFKGQHFDFL 444
           +  ++ GY +P  +QVLVN WAIGRD   W DA  F PERF+E    +  D +G+ F+ +
Sbjct: 384 QSVEVCGYNVPKGSQVLVNAWAIGRDETVWDDALAFKPERFME----SELDIRGRDFELI 443

Query: 445 PFGSGRRMCPAVPLASRVLPVALGSLILSFDWALPDGMLPEEMDMSEQMGITLRKKVPLK 504
           PFG+GRR+CP +PLA R +P+ LGSL+ SF+W L  GM P+++DM E+ GITL+K  PL+
Sbjct: 444 PFGAGRRICPGLPLALRTVPLMLGSLLNSFNWKLEGGMAPKDLDMEEKFGITLQKAHPLR 491

Query: 505 AIPT 509
           A+P+
Sbjct: 504 AVPS 491

BLAST of Spg033282 vs. ExPASy Swiss-Prot
Match: D1MI46 (Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.4e-105
Identity = 208/485 (42.89%), Postives = 287/485 (59.18%), Query Frame = 0

Query: 25  SAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPIMTLHLGSMSTVVVSSSTAA 84
           S +++ LPPGP   P++GNL  L    H+S  KLA K+GPIM L LG ++T+VV+SS  A
Sbjct: 26  SRKSKNLPPGPSPLPLIGNLHLLGDQPHKSLAKLAKKHGPIMGLQLGQVTTIVVTSSGMA 85

Query: 85  REMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRMLRRLATTEFFVTRRLDAMR 144
           +E+ +  D   + R I  A+  +   + S+I       WR LR+   +  F   RLDA +
Sbjct: 86  KEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVASRWRGLRKALNSNMFSGNRLDANQ 145

Query: 145 HIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLFFIMSFNLIGNLIFSKDLVEPNSKRG 204
           H+R R V  +I +    +  G       I++G+  F  S NL+ N +FSKDL +P S   
Sbjct: 146 HLRSRKVQELIAYCRKSSQTG-----DAIDVGRAAFRTSLNLLSNTMFSKDLTDPYSDSA 205

Query: 205 KGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRERLKEK 264
           K F      VM  AGKPN+ D+ P    VDPQGIR++   H  K  E+ G  I ERL++K
Sbjct: 206 KEFKDLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKILELFGGLIDERLQQK 265

Query: 265 ESDERKEEGRKDYLDVLLEFRGDGVEEPSCFSSWIINVIVFEMFTAGTDTTTSTLEWAMT 324
           ++    +    D LDVLL       E P       I  +  ++F AGTDTT+STLEWAM+
Sbjct: 266 KAKGVND----DVLDVLLT---TSEESPEEIDRTHIQRMCLDLFVAGTDTTSSTLEWAMS 325

Query: 325 ELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAM 384
           E+L NP  ++  QAEL   I     +EE  L  LPYL   +KETLR+HPP+P L PR   
Sbjct: 326 EMLKNPEKMKAAQAELAQVIGKGKAVEEADLARLPYLRCAIKETLRIHPPVPLLIPRRTE 385

Query: 385 KPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATVFSPERFLEPGAGAGKDFKGQHFDFL 444
           +  ++ GY +P  +QVLVNVWAI RD   WKD   F PERFLE    +  + +G+ F+ +
Sbjct: 386 QEVEVCGYTVPKNSQVLVNVWAISRDDAIWKDPLSFKPERFLE----SELEMRGKDFELI 445

Query: 445 PFGSGRRMCPAVPLASRVLPVALGSLILSFDWALPDGMLPEEMDMSEQMGITLRKKVPLK 504
           PFG+GRR+CP +PLA R++PV LGSL+ SFDW L  G+ P+++DM E+ GITL+K  PL+
Sbjct: 446 PFGAGRRICPGLPLAVRMVPVMLGSLLNSFDWKLEGGIAPKDLDMEEKFGITLQKAHPLR 494

Query: 505 AIPTP 510
           A+ TP
Sbjct: 506 AVATP 494

BLAST of Spg033282 vs. ExPASy TrEMBL
Match: A0A6J1F4L5 (cytochrome P450 76A1 OS=Cucurbita moschata OX=3662 GN=LOC111442113 PE=3 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 5.2e-250
Identity = 436/514 (84.82%), Postives = 457/514 (88.91%), Query Frame = 0

Query: 1   MEFEIAGFIFASILWGFWVWRRRGSAE-AEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I  S LWG WVWRRRG+A   EQLPPGP RWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAALILVSFLWGLWVWRRRGAAALGEQLPPGPTRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDAPL+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDAPLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAMI++VE E    A    PP+++GK F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMIQYVEDEIKGSAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV PNSKRGK FYYH RRVME AG PNVADFL  FRW DPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMETAGTPNVADFLRVFRWFDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESDERKEEGRKDYLDVLLEFRGDGVEEPSCFSSWI 300
           R TQFHVK+AFEIAGEFI+ER  EK   E+KEE +KDYLDVLLEFRGDGV EPS FSSWI
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSMEK---EKKEERKKDYLDVLLEFRGDGVVEPSRFSSWI 300

Query: 301 INVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLP 360
           INVIV EMFTAGTDTTTS+LEWAM ELL NPAA+QKLQAELRSTIPPDG LEETHLQNLP
Sbjct: 301 INVIVLEMFTAGTDTTTSSLEWAMAELLRNPAAMQKLQAELRSTIPPDGKLEETHLQNLP 360

Query: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATV 420
           YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGY IPAETQVLVNVWAIGRDPKNWKDA V
Sbjct: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYWIPAETQVLVNVWAIGRDPKNWKDALV 420

Query: 421 FSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALP 480
           FSPERFLEP     KDFKGQHFDFLPFGSGRRMCPAVPLASRVLP+ALGSLIL FDWALP
Sbjct: 421 FSPERFLEP--TGSKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPMALGSLILRFDWALP 480

Query: 481 DGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRR 514
           DG+LPEEMDMSEQMGITL+KKVPLKAIP P+KRR
Sbjct: 481 DGLLPEEMDMSEQMGITLKKKVPLKAIPIPHKRR 509

BLAST of Spg033282 vs. ExPASy TrEMBL
Match: A0A6J1IXV7 (cytochrome P450 76A2 OS=Cucurbita maxima OX=3661 GN=LOC111481619 PE=3 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.2e-249
Identity = 436/514 (84.82%), Postives = 458/514 (89.11%), Query Frame = 0

Query: 1   MEFEIAGFIFASILWGFWVWRRRGSAE-AEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME EIA  I  S LWG WVWRRRG+A   EQLPPGPPRWPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MEIEIAALILVSFLWGLWVWRRRGAAALGEQLPPGPPRWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFKNHDA L+GRMIYEAMKG+HGTEGSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTIAREMFKNHDASLSGRMIYEAMKGDHGTEGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLF 180
           G HWRMLRRLAT+EFFVTRRLDAM+H+RRRCVDAMI++VE E    A    PP+++GK F
Sbjct: 121 GSHWRMLRRLATSEFFVTRRLDAMQHVRRRCVDAMIQYVEDEIKGSAYWYAPPMDVGKFF 180

Query: 181 FIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           FIMSFNLIGNLIFSKDLV PNSKRGK FYYH RRVME+AG PNVADFL  FRWVDPQGIR
Sbjct: 181 FIMSFNLIGNLIFSKDLVGPNSKRGKEFYYHTRRVMEIAGTPNVADFLRVFRWVDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERLKEKESDERKEEGRKDYLDVLLEFRGDGVEEPSCFSSWI 300
           R TQFHVK+AFEIAGEFI+ER  EK   E+KEE +KDYLDVLLEFRGDGV EPS FSSWI
Sbjct: 241 RTTQFHVKEAFEIAGEFIKERSTEK---EKKEERKKDYLDVLLEFRGDGVVEPSRFSSWI 300

Query: 301 INVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLP 360
           INVIV EMFTAGTDTTTS+LEWAM ELL NPAA+QKLQAELRSTI PDG LEETHLQNLP
Sbjct: 301 INVIVLEMFTAGTDTTTSSLEWAMAELLRNPAAMQKLQAELRSTILPDGKLEETHLQNLP 360

Query: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATV 420
           YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGY IPAETQVLVNVWAIGRDPKNWKDA V
Sbjct: 361 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYWIPAETQVLVNVWAIGRDPKNWKDALV 420

Query: 421 FSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALP 480
           FSPERFLEP     KDFKGQHFDFLPFGSGRRMCPAVPLASRVLP+ALGSLIL FDWALP
Sbjct: 421 FSPERFLEP--TGSKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPMALGSLILRFDWALP 480

Query: 481 DGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRR 514
           DG+LPEEMDMSEQMGITL+KKVPLKAIP P+KRR
Sbjct: 481 DGLLPEEMDMSEQMGITLKKKVPLKAIPIPHKRR 509

BLAST of Spg033282 vs. ExPASy TrEMBL
Match: A0A0A0LEE2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G748270 PE=3 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.2e-249
Identity = 430/525 (81.90%), Postives = 465/525 (88.57%), Query Frame = 0

Query: 1   MEFE-IAGFIFASILWGFWVWRRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLA 60
           ME E IAGFI    LWG W+WR RG+    QLPPGPP WPVVGNL QLSFSAHESFTKLA
Sbjct: 1   MELEIIAGFILLLFLWGLWLWRCRGAGADRQLPPGPPCWPVVGNLLQLSFSAHESFTKLA 60

Query: 61  TKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQY 120
            KYGPIMTL LGSM TVVVSSST AREMFK HD PL+GRMIYEAMKGNHGT+GSLITSQY
Sbjct: 61  AKYGPIMTLQLGSMKTVVVSSSTVAREMFKTHDVPLSGRMIYEAMKGNHGTQGSLITSQY 120

Query: 121 GPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLF 180
           GPHWRMLRRLAT+EFFV R+L++M+H+RR C+DAMIRH  VE G   CG+ P +++GK  
Sbjct: 121 GPHWRMLRRLATSEFFVARQLESMKHVRRHCIDAMIRH--VEEGCDPCGNAPALDVGKFV 180

Query: 181 FIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIR 240
           F+M FNLIGNLIFSKDL+E NSKRGK FYYH R+VMEMAGKPNVADFLPA RW+DPQGIR
Sbjct: 181 FVMGFNLIGNLIFSKDLLEANSKRGKEFYYHTRKVMEMAGKPNVADFLPALRWLDPQGIR 240

Query: 241 RKTQFHVKKAFEIAGEFIRERL--KEK------ESDERKEE-GRKDYLDVLLEFRGDGVE 300
           RKTQFHV KAFEIAGEFI+ERL  +EK      + DE+KEE G+KDYLDVLLEFRGDGVE
Sbjct: 241 RKTQFHVNKAFEIAGEFIKERLLIREKRDIINDDDDEKKEESGKKDYLDVLLEFRGDGVE 300

Query: 301 EPSCFSSWIINVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNL 360
           EPS FSSWIINVIV EMFTAGTDTTTSTLEWAM ELLHNPA+LQKLQAELR+TI P+ NL
Sbjct: 301 EPSRFSSWIINVIVLEMFTAGTDTTTSTLEWAMAELLHNPASLQKLQAELRATIAPNDNL 360

Query: 361 EETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRD 420
           EETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPC ILGYRIPAETQVLVN WAIGRD
Sbjct: 361 EETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCNILGYRIPAETQVLVNFWAIGRD 420

Query: 421 PKNWKDATVFSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSL 480
           PKNWKDA VFSPERF +P +G+ KDFKGQHFDF+PFGSGRRMCPAVPLASRVLP+ALGSL
Sbjct: 421 PKNWKDALVFSPERFFDPSSGS-KDFKGQHFDFIPFGSGRRMCPAVPLASRVLPMALGSL 480

Query: 481 ILSFDWALPDGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRRRF 516
           ILSFDWALPDG+ P+EMDMSEQMGITLRKKVPLKAIPTP+KRRRF
Sbjct: 481 ILSFDWALPDGVSPKEMDMSEQMGITLRKKVPLKAIPTPHKRRRF 522

BLAST of Spg033282 vs. ExPASy TrEMBL
Match: A0A1S3B621 (LOW QUALITY PROTEIN: cytochrome P450 76A2 OS=Cucumis melo OX=3656 GN=LOC103486563 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 8.1e-243
Identity = 418/523 (79.92%), Postives = 457/523 (87.38%), Query Frame = 0

Query: 1   MEFEIAGFIFASILWGFWVWRRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLAT 60
           ME EI       +L   W+WR R +   EQLPPGPP +PVVGNL QLSFSAHESFTKLA 
Sbjct: 1   MELEIIACFL--LLLFLWLWRCRRAGAREQLPPGPPCFPVVGNLLQLSFSAHESFTKLAA 60

Query: 61  KYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLITSQYG 120
           KYGPIMTL LGSM TVV+SSSTAAREMFK HDAPL+GRMIYEAMKG+HGT+GSLITSQYG
Sbjct: 61  KYGPIMTLQLGSMKTVVISSSTAAREMFKTHDAPLSGRMIYEAMKGDHGTQGSLITSQYG 120

Query: 121 PHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLFF 180
           PHWRMLRRLAT+EFFV R+L++M+H+RR C+DAMIRH  V+ G   CG+   +++GK  F
Sbjct: 121 PHWRMLRRLATSEFFVARQLESMKHVRRHCIDAMIRH--VQEGCDPCGNAQALDVGKFMF 180

Query: 181 IMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRR 240
           +MSFNLIGNLIFSKDL+E NSKRGK FYYH R+VMEMAGKPNVADFLPA RW+DPQGIRR
Sbjct: 181 VMSFNLIGNLIFSKDLLEANSKRGKEFYYHTRKVMEMAGKPNVADFLPALRWLDPQGIRR 240

Query: 241 KTQFHVKKAFEIAGEFIRERL---------KEKESDERKEEGRKDYLDVLLEFRGDGVEE 300
           KTQFHV KAFEIAGEFI+ERL            + DE+ ++ +KDYLDVLLEFRGDGVEE
Sbjct: 241 KTQFHVNKAFEIAGEFIKERLLIRGKKDIINNDDDDEKXKKRKKDYLDVLLEFRGDGVEE 300

Query: 301 PSCFSSWIINVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLE 360
           PSCFSSWIINVIVFEMFTAGTDTTTSTLEWAM ELLHNPAALQKLQAELR+TI P+ NLE
Sbjct: 301 PSCFSSWIINVIVFEMFTAGTDTTTSTLEWAMAELLHNPAALQKLQAELRTTIAPNENLE 360

Query: 361 ETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDP 420
           ETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPC ILGYRIPAETQVLVN WAIGRDP
Sbjct: 361 ETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCNILGYRIPAETQVLVNFWAIGRDP 420

Query: 421 KNWKDATVFSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLI 480
           KNW+DA VFSPERF +P    GKDFKGQHFDF+PFGSGRRMCPAVPLASRVLP+ALGSLI
Sbjct: 421 KNWEDALVFSPERFFDP--SGGKDFKGQHFDFIPFGSGRRMCPAVPLASRVLPMALGSLI 480

Query: 481 LSFDWALPDGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRRR 515
           LSFDWALPDG+LP+EMDMSEQMGITLRKKVPLKAIPTP+KRRR
Sbjct: 481 LSFDWALPDGVLPKEMDMSEQMGITLRKKVPLKAIPTPHKRRR 517

BLAST of Spg033282 vs. ExPASy TrEMBL
Match: A0A2P5C548 (Cytochrome P450, E-class, group I OS=Parasponia andersonii OX=3476 GN=PanWU01x14_183230 PE=3 SV=1)

HSP 1 Score: 718.4 bits (1853), Expect = 2.2e-203
Identity = 355/520 (68.27%), Postives = 417/520 (80.19%), Query Frame = 0

Query: 1   MEFEIAGFIFASILWGFWVW----RRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFT 60
           M++E+ G   A +LW  W      R     +  +LPPGP  WPVVGN+ QL +S HESF 
Sbjct: 1   MDYEVLGLFMAVLLWVAWALITERRHHQLEDLGRLPPGPRWWPVVGNIFQLGWSPHESFA 60

Query: 61  KLATKYGPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLIT 120
           KLA  +GPIMTL LGSMSTVV+SS   AREMFKNHD  LAGR IYEAMKG+HG EGSLIT
Sbjct: 61  KLARTHGPIMTLWLGSMSTVVISSQEVAREMFKNHDVVLAGRKIYEAMKGDHGNEGSLIT 120

Query: 121 SQYGPHWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELG 180
           +QYGPHWRMLRRL+TTEFFVT RLDA R +R RC+D M+R +E EA  GACG + P+++G
Sbjct: 121 AQYGPHWRMLRRLSTTEFFVTSRLDATRGVRERCIDRMVRFIE-EA--GACGSK-PVDVG 180

Query: 181 KLFFIMSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQ 240
           + FF+M+FNLIGNL+FSKDL++P S+ G  F++HA +VME+AGKPNVADFLP  RWVDPQ
Sbjct: 181 RFFFLMAFNLIGNLMFSKDLLDPKSESGAKFFFHAGKVMELAGKPNVADFLPILRWVDPQ 240

Query: 241 GIRRKTQFHVKKAFEIAGEFIRERLKEKESDERKE-EGRKDYLDVLLEFRGDGVEEPSCF 300
           GIRRKTQFHV++AFEIAG FI ER++  E +E ++ + RKD+LDVLLEFRG+GVEEPS F
Sbjct: 241 GIRRKTQFHVERAFEIAGGFIMERMESMEKEETEDKDKRKDFLDVLLEFRGNGVEEPSKF 300

Query: 301 SSWIINVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHL 360
           SS  INVIVFEMFTAGTDTTTSTLEWAM ELLHNP AL+K+Q+ELR TI P   L+E  +
Sbjct: 301 SSRTINVIVFEMFTAGTDTTTSTLEWAMAELLHNPKALKKVQSELRRTINPGEKLDERDI 360

Query: 361 QNLPYLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWK 420
           +NLPYL AV+KETLRLHPPLPFL P MAM  CK+LGY IP ETQ+LVNVWAIGRDPK W 
Sbjct: 361 ENLPYLKAVIKETLRLHPPLPFLVPHMAMDACKMLGYYIPKETQILVNVWAIGRDPKTWD 420

Query: 421 DATVFSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFD 480
           D   F P+RFLEP     +D+KG HF+F+PFGSGRRMCPAVPLASRVLP+ALGSL+LSFD
Sbjct: 421 DPLKFKPKRFLEPNM---RDYKGHHFEFIPFGSGRRMCPAVPLASRVLPLALGSLLLSFD 480

Query: 481 WALPDGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRRRF 516
           W L DGM P++MDMSE+MGITLRK VPLK IP P+K   F
Sbjct: 481 WVLADGMKPDDMDMSERMGITLRKAVPLKVIPIPFKGGHF 513

BLAST of Spg033282 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 628.6 bits (1620), Expect = 4.3e-180
Identity = 323/488 (66.19%), Postives = 382/488 (78.28%), Query Frame = 0

Query: 30  QLPPGPPRWPVVGNLPQLS-FSAHESFTKLATKYGPIMTLHLGSMSTVVVSSSTAAREMF 89
           +LPPGP  WPV+GN+ QL+    H+S TKL+ ++GPIMTL +GSM TVV+SSS  ARE+F
Sbjct: 35  RLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIF 94

Query: 90  KNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRMLRRLATTEFFVTRRLDAMRHIRR 149
           K HDA LAGR IYEAMKG   ++GSLIT+QYG +WRMLRRL TT+FFVTRRLDAM  +R 
Sbjct: 95  KKHDAALAGRKIYEAMKGGKSSDGSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVRS 154

Query: 150 RCVDAMIRHVEVEAGIGACGDEPPIELGKLFFIMSFNLIGNLIFSKDLVEPNSKRGKGFY 209
           RCVD M+R VE     G       I++G+ FF+M+FNLIGNL+FS+DL++P+SKRG  F+
Sbjct: 155 RCVDQMLRFVEE----GGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLDPDSKRGSEFF 214

Query: 210 YHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRERLKEKESDE 269
           YH  +VME AGKPNVADF P  R++DPQGIRRKTQFHV+KAFEIAGEFIRER + +E  E
Sbjct: 215 YHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVRER-E 274

Query: 270 RKEEGRKDYLDVLLEFR-GDGV-EEPSCFSSWIINVIVFEMFTAGTDTTTSTLEWAMTEL 329
           + +E  KDYLDVLLEFR GDGV EEPS FSS  INVIVFEMFTAGTDTTTSTLEWA+ EL
Sbjct: 275 KSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAEL 334

Query: 330 LHNPAALQKLQAELRSTIPPDG-NLEETHLQNLPYLDAVVKETLRLHPPLPFLFPRMAMK 389
           LHNP  L KLQ ELR+        L+E  L NLPYL AV+ ETLRLHPPLPFL P  AM 
Sbjct: 335 LHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMS 394

Query: 390 PCKILG-YRIPAETQVLVNVWAIGRDPKNWKDATVFSPERFL-EPGAGAGKDFKGQHFDF 449
            C I   Y IP ETQVLVNVWAIGRDPK W D  +F PERF+ +P A   +DFKGQ ++F
Sbjct: 395 TCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPDA---RDFKGQDYEF 454

Query: 450 LPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALPDGMLPEEMDMSEQMGITLRKKVPL 509
           LPFGSGRRMCPA+PLASRVLP+A+GS++ SFDWAL +G+  EEMDM E++GITL+K VPL
Sbjct: 455 LPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPL 514

Query: 510 KAIPTPYK 512
           +AIP PY+
Sbjct: 515 EAIPIPYR 514

BLAST of Spg033282 vs. TAIR 10
Match: AT3G52970.2 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 622.5 bits (1604), Expect = 3.1e-178
Identity = 326/499 (65.33%), Postives = 385/499 (77.15%), Query Frame = 0

Query: 30  QLPPGPPRWPVVGNLPQLS-FSAHESFTKLATKYGPIMTLHLGSMSTVVVSSSTAAREMF 89
           +LPPGP  WPV+GN+ QL+    H+S TKL+ ++GPIMTL +GSM TVV+SSS  ARE+F
Sbjct: 35  RLPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIF 94

Query: 90  KNHDAPLAGRMIYEAMKGNHGTEGSLITSQYGPHWRMLRRLATTEFFVTRRLDAMRHIRR 149
           K HDA LAGR IYEAMKG   ++GSLIT+QYG +WRMLRRL TT+FFVTRRLDAM  +R 
Sbjct: 95  KKHDAALAGRKIYEAMKGGKSSDGSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVRS 154

Query: 150 RCVDAMIRHVEVEAG----------IGAC-GDEPPIELGKLFFIMSFNLIGNLIFSKDLV 209
           RCVD M+R VE E G          I  C      I++G+ FF+M+FNLIGNL+FS+DL+
Sbjct: 155 RCVDQMLRFVE-EGGQNGMYYTINNIMTCIICTKTIDVGRYFFLMAFNLIGNLMFSRDLL 214

Query: 210 EPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFI 269
           +P+SKRG  F+YH  +VME AGKPNVADF P  R++DPQGIRRKTQFHV+KAFEIAGEFI
Sbjct: 215 DPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGEFI 274

Query: 270 RERLKEKESDERKEEGRKDYLDVLLEFR-GDGV-EEPSCFSSWIINVIVFEMFTAGTDTT 329
           RER + +E  E+ +E  KDYLDVLLEFR GDGV EEPS FSS  INVIVFEMFTAGTDTT
Sbjct: 275 RERTEVRER-EKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTT 334

Query: 330 TSTLEWAMTELLHNPAALQKLQAELRSTIPPDG-NLEETHLQNLPYLDAVVKETLRLHPP 389
           TSTLEWA+ ELLHNP  L KLQ ELR+        L+E  L NLPYL AV+ ETLRLHPP
Sbjct: 335 TSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPP 394

Query: 390 LPFLFPRMAMKPCKILG-YRIPAETQVLVNVWAIGRDPKNWKDATVFSPERFL-EPGAGA 449
           LPFL P  AM  C I   Y IP ETQVLVNVWAIGRDPK W D  +F PERF+ +P A  
Sbjct: 395 LPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISDPDA-- 454

Query: 450 GKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALPDGMLPEEMDMSEQ 509
            +DFKGQ ++FLPFGSGRRMCPA+PLASRVLP+A+GS++ SFDWAL +G+  EEMDM E+
Sbjct: 455 -RDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGER 514

Query: 510 MGITLRKKVPLKAIPTPYK 512
           +GITL+K VPL+AIP PY+
Sbjct: 515 IGITLKKAVPLEAIPIPYR 528

BLAST of Spg033282 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 372.1 bits (954), Expect = 7.3e-103
Identity = 199/488 (40.78%), Postives = 295/488 (60.45%), Query Frame = 0

Query: 23  RGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKYGPIMTLHLGSMSTVVVSSST 82
           R S  A  LPPGPPR P++GN+  +    H SF +L+  YGP+M+L LGS++TVV++S  
Sbjct: 30  RISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPE 89

Query: 83  AAREMFKNHDAPLAGRMIYEAMKGNHGTEGSLI-TSQYGPHWRMLRRLATTEFFVTRRLD 142
           AARE+ + HD  L+ R    A++  +  + SL+        WR+LRRL+ T+    +R++
Sbjct: 90  AAREVLRTHDQILSARSPTNAVRSINHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIE 149

Query: 143 AMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLFFIMSFNLIGNLIFSKDLVEPNS 202
           A + +R   V  ++  +   +       E  +++ ++ FI + N+I N++FS DL   N+
Sbjct: 150 ATKALRMNKVKELVSFISESS-----DREESVDISRVAFITTLNIISNILFSVDLGSYNA 209

Query: 203 KRG-KGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRKTQFHVKKAFEIAGEFIRER 262
           K    G       VM+ AG P+ A++ P  R++D QG  +  +   ++   +   FI  +
Sbjct: 210 KASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAK 269

Query: 263 LKEKESDER-KEEGRKDYLDVLLEFRGDGVEEPSCFSSWIINVIVFEMFTAGTDTTTSTL 322
           + EK S    K+  + D++D LL+++GD  E     S   I  ++ +MFTAGTDT++STL
Sbjct: 270 IAEKSSQNNPKDVSKNDFVDNLLDYKGDESE----LSISDIEHLLLDMFTAGTDTSSSTL 329

Query: 323 EWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLPYLDAVVKETLRLHPPLPFLF 382
           EWAMTELL NP  + K QAE+   I  +G +EE+ +  LPYL AVVKET RLH P+P L 
Sbjct: 330 EWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQAVVKETFRLHTPVPLLI 389

Query: 383 PRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATVFSPERFLEPGAGAGKDFKGQ 442
           PR A    +ILG+ +  +TQVLVNVWAIGRDP  W + + F PERFL    G   D +G+
Sbjct: 390 PRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERFL----GKDMDVRGR 449

Query: 443 HFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALPDGMLPEEMDMSEQMGITLRK 502
            ++  PFG+GRR+CP +PLA + + + L SL+ SFDW LP G+L E++DM E  G+TL K
Sbjct: 450 DYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSEDLDMDETFGLTLHK 504

Query: 503 KVPLKAIP 508
             PL A+P
Sbjct: 510 TNPLHAVP 504

BLAST of Spg033282 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 359.4 bits (921), Expect = 4.9e-99
Identity = 198/508 (38.98%), Postives = 299/508 (58.86%), Query Frame = 0

Query: 3   FEIAGFIFASILWGFWVWRRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKY 62
           F I  FI + +L+      RR   +  + PPGPPR P++GN+  +  + H SFT L+  Y
Sbjct: 10  FLIFCFILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTY 69

Query: 63  GPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKGNHGTEGSL-ITSQYGP 122
           GP+M+L LG +++VV++S  A RE+ K HD  L+GR I EA K N+  E S+        
Sbjct: 70  GPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNNHHEFSVGWIHPSSS 129

Query: 123 HWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLFFI 182
            +RMLR+L+ T+ F  + + A + +R + V  ++  +       +C  E  +++  + F+
Sbjct: 130 RFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLS-----ESCEREEAVDISHVSFV 189

Query: 183 MSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRK 242
            + N+I N++FS +L   +SK    F        E  G P++A+F P  R++D QG  +K
Sbjct: 190 TALNIISNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKK 249

Query: 243 TQFHVKKAFEIAGEFIRERLKEKES-DERKEEGRKDYLDVLLEF-RGDGVEEPSCFSSWI 302
            +    +  ++  EF   R+ EK S    K+   KD+LDVL++  +GD  E     +   
Sbjct: 250 MRESSGRLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETE----INIDE 309

Query: 303 INVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNLP 362
           I  ++ +MF AGTDT +ST+EWAM ELL NP  + K+Q E+   I  +G+ +E+ +  LP
Sbjct: 310 IEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESDISKLP 369

Query: 363 YLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDATV 422
           YL AVVKET RLHP  PFL  R A    +ILG+ +  ++QVLVNVWAIGRDP  W++ T 
Sbjct: 370 YLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTH 429

Query: 423 FSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWALP 482
           F PERFL    G   D KG  ++  PFG+GRR+CP +PLA + + + L SL+ +F+W LP
Sbjct: 430 FEPERFL----GKEIDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLP 489

Query: 483 DGMLPEEMDMSEQMGITLRKKVPLKAIP 508
           +G+  E++DM E  G+T+ K  PL A+P
Sbjct: 490 NGVGSEDLDMEETFGLTVHKTNPLLAVP 504

BLAST of Spg033282 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 359.4 bits (921), Expect = 4.9e-99
Identity = 197/515 (38.25%), Postives = 299/515 (58.06%), Query Frame = 0

Query: 3   FEIAGFIFASILWGFWVWRRRGSAEAEQLPPGPPRWPVVGNLPQLSFSAHESFTKLATKY 62
           F +  F+ +  +  F   R R S +    PPGPPR P++GN+  +  + H SF  L+  Y
Sbjct: 10  FPLFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTY 69

Query: 63  GPIMTLHLGSMSTVVVSSSTAAREMFKNHDAPLAGRMIYEAMKG-NHGTEGSLITSQYGP 122
           GPIM+L  GS++TVVV+S  AARE+ + +D  L+ R    +++  NH     +       
Sbjct: 70  GPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSINHDKVSVVWLPPSSS 129

Query: 123 HWRMLRRLATTEFFVTRRLDAMRHIRRRCVDAMIRHVEVEAGIGACGDEPPIELGKLFFI 182
            WR+LR+L+ T+ F  +R++A + +R   V  ++  +   +       E  +++ +  FI
Sbjct: 130 RWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESS-----EREEAVDISRATFI 189

Query: 183 MSFNLIGNLIFSKDLVEPNSKRGKGFYYHARRVMEMAGKPNVADFLPAFRWVDPQGIRRK 242
            + N+I N++FS DL   +S +   F      VME  G P+ A+F P   ++D QG R+ 
Sbjct: 190 TALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKT 249

Query: 243 TQFHVKKAFEIAGEFIRERLKEK--ESDERKEEGRKDYLDVLLEF-RGDGVEEPSCFSSW 302
            +   ++ F++   FI  +L EK       K+   +D++DVLL+   GD  E     ++ 
Sbjct: 250 LKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAE----LNTN 309

Query: 303 IINVIVFEMFTAGTDTTTSTLEWAMTELLHNPAALQKLQAELRSTIPPDGNLEETHLQNL 362
            I  ++ ++F AGTDT +ST+EWAM ELL NP  + K QAE+   I   G +EE+ +  L
Sbjct: 310 DIVHLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISAL 369

Query: 363 PYLDAVVKETLRLHPPLPFLFPRMAMKPCKILGYRIPAETQVLVNVWAIGRDPKNWKDAT 422
           PYL AVVKET RLHP  P L PR A    ++LG+ +P +TQV VNVWAIGRDP  W++++
Sbjct: 370 PYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSS 429

Query: 423 VFSPERFLEPGAGAGKDFKGQHFDFLPFGSGRRMCPAVPLASRVLPVALGSLILSFDWAL 482
            F PERFL    G   D +G+ ++  PFG+GRR+CP +PLA + +P+ L SL+ SFDW L
Sbjct: 430 RFKPERFL----GKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKL 489

Query: 483 PDGMLPEEMDMSEQMGITLRKKVPLKAIPTPYKRR 514
           P+G+  E++DM E  G+TL K  PL A+P   + R
Sbjct: 490 PNGVGSEDLDMDETFGLTLHKTNPLHAVPVKKRGR 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022935159.11.1e-24984.82cytochrome P450 76A1 [Cucurbita moschata][more]
XP_022982917.12.4e-24984.82cytochrome P450 76A2 [Cucurbita maxima][more]
XP_004138005.12.4e-24981.90iridoid oxidase [Cucumis sativus] >KGN59047.1 hypothetical protein Csa_001475 [C... [more]
XP_023528102.15.4e-24984.24cytochrome P450 76A2 [Cucurbita pepo subsp. pepo][more]
XP_038905939.11.8e-24481.07iridoid oxidase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
W8JIS56.6e-12542.94Iridoid oxidase OS=Catharanthus roseus OX=4058 GN=IO PE=1 SV=1[more]
P371221.3e-12043.25Cytochrome P450 76A2 OS=Solanum melongena OX=4111 GN=CYP76A2 PE=2 SV=1[more]
P371215.8e-11345.42Cytochrome P450 76A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP76A1 PE=2 SV=... [more]
Q8VWZ73.6e-10741.94Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1[more]
D1MI463.4e-10542.89Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F4L55.2e-25084.82cytochrome P450 76A1 OS=Cucurbita moschata OX=3662 GN=LOC111442113 PE=3 SV=1[more]
A0A6J1IXV71.2e-24984.82cytochrome P450 76A2 OS=Cucurbita maxima OX=3661 GN=LOC111481619 PE=3 SV=1[more]
A0A0A0LEE21.2e-24981.90Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G748270 PE=3 SV=1[more]
A0A1S3B6218.1e-24379.92LOW QUALITY PROTEIN: cytochrome P450 76A2 OS=Cucumis melo OX=3656 GN=LOC10348656... [more]
A0A2P5C5482.2e-20368.27Cytochrome P450, E-class, group I OS=Parasponia andersonii OX=3476 GN=PanWU01x14... [more]
Match NameE-valueIdentityDescription
AT3G52970.14.3e-18066.19cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
AT3G52970.23.1e-17865.33cytochrome P450, family 76, subfamily G, polypeptide 1 [more]
AT2G45560.17.3e-10340.78cytochrome P450, family 76, subfamily C, polypeptide 1 [more]
AT1G33720.14.9e-9938.98cytochrome P450, family 76, subfamily C, polypeptide 6 [more]
AT2G45570.14.9e-9938.25cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 83..104
score: 20.17
coord: 319..345
score: 30.73
coord: 362..380
score: 40.21
coord: 182..200
score: 18.64
coord: 443..453
score: 58.45
coord: 299..316
score: 34.5
coord: 453..476
score: 27.12
coord: 59..78
score: 39.26
coord: 403..427
score: 38.08
IPR001128Cytochrome P450PRINTSPR00385P450coord: 363..374
score: 49.25
coord: 444..453
score: 59.44
coord: 310..327
score: 43.45
IPR001128Cytochrome P450PFAMPF00067p450coord: 32..497
e-value: 1.0E-98
score: 331.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 19..506
e-value: 7.8E-124
score: 415.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 32..509
NoneNo IPR availablePANTHERPTHR24299:SF22CYTOCHROME P450 76A1-LIKEcoord: 1..510
NoneNo IPR availablePANTHERPTHR24299CYTOCHROME P450 FAMILY 1coord: 1..510
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 446..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg033282.1Spg033282.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen