Spg033229 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg033229
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Adenine nucleotide alpha hydrolases-like superfamily protein
Location	scaffold5: 1600978 .. 1601447 (+)
RNA-Seq Expression	Spg033229
Synteny	Spg033229

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGCCCATTGGACTCCAGGTAAACGAGTGAACCTTAATCCAGAAGACGAGCAATTGCTGACTCATCGTACACGTGGACGGCGGAGATGCAATTCCATGTTCCACATCAACTTTTCAAAGAAATGGTTCCTTATTTCCGTCTGACGCTTGTCCCGTTATTGGCATTTTAAAGCAAACCCAGGTTCCGTTCCTCGTTGTCAGTTCTCTCACGTTTTCCTTTCATTCGCTCGCACTAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCAGCAGCAGCCACAGAAAGAAATGGTGAAGGATCGGAAGATCGGAGTGGCTCTTGATTTCTCCAACAGCAGCAAAAACGCTCTCAAATGGGCCATCGATAACTTGGCCGACAAAGGCGACACTCTCTACATCATCTACGTTAACCCCAATTCACTCGACGAATCTGCCCATCACCTCTGGGCTCAATCCGGCTCTCGTCAGTAA

mRNA sequence

ATGAGGCCCATTGGACTCCAGTTCTCTCACGTTTTCCTTTCATTCGCTCGCACTAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCAGCAGCAGCCACAGAAAGAAATGGTGAAGGATCGGAAGATCGGAGTGGCTCTTGATTTCTCCAACAGCAGCAAAAACGCTCTCAAATGGGCCATCGATAACTTGGCCGACAAAGGCGACACTCTCTACATCATCTACGTTAACCCCAATTCACTCGACGAATCTGCCCATCACCTCTGGGCTCAATCCGGCTCTCGTCAGTAA

Coding sequence (CDS)

ATGAGGCCCATTGGACTCCAGTTCTCTCACGTTTTCCTTTCATTCGCTCGCACTAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCAGCAGCAGCCACAGAAAGAAATGGTGAAGGATCGGAAGATCGGAGTGGCTCTTGATTTCTCCAACAGCAGCAAAAACGCTCTCAAATGGGCCATCGATAACTTGGCCGACAAAGGCGACACTCTCTACATCATCTACGTTAACCCCAATTCACTCGACGAATCTGCCCATCACCTCTGGGCTCAATCCGGCTCTCGTCAGTAA

Protein sequence

MRPIGLQFSHVFLSFARTKKKKKKKKKKQQQPQKEMVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGSRQ

Homology

[Close]

BLAST of Spg033229 vs. NCBI nr
Match: XP_038903504.1 (universal stress protein PHOS32 [Benincasa hispida])

HSP 1 Score: 114.4 bits (285), Expect = 5.4e-22
Identity = 54/58 (93.10%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          MVKDRKIGVALDFSNSSKNAL+WAIDNLADKGDTL+IIYVNPNSLDESAH LWA+SGS
Sbjct: 1  MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLDESAHRLWAESGS 58

[Close]

BLAST of Spg033229 vs. NCBI nr
Match: XP_008442491.1 (PREDICTED: uncharacterized protein C167.05 [Cucumis melo] >KAA0044129.1 universal stress protein PHOS32-like [Cucumis melo var. makuwa] >TYK25009.1 universal stress protein PHOS32-like [Cucumis melo var. makuwa])

HSP 1 Score: 113.2 bits (282), Expect = 1.2e-21
Identity = 54/58 (93.10%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          MVKDRKIGVALDFS+SSKNALKWAIDNLADKGDTLYIIYVNPNSL+ESAH LWA+SGS
Sbjct: 1  MVKDRKIGVALDFSDSSKNALKWAIDNLADKGDTLYIIYVNPNSLNESAHRLWAESGS 58

[Close]

BLAST of Spg033229 vs. NCBI nr
Match: XP_011651876.1 (universal stress protein PHOS32 [Cucumis sativus] >KGN58822.1 hypothetical protein Csa_001140 [Cucumis sativus])

HSP 1 Score: 112.8 bits (281), Expect = 1.6e-21
Identity = 53/58 (91.38%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          MVKDRKIGVALDFSNSSKNAL+WAIDNLADKGDTL+IIYVNPNSL+ESAH LWA+SGS
Sbjct: 1  MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLEESAHRLWAESGS 58

[Close]

BLAST of Spg033229 vs. NCBI nr
Match: XP_022145652.1 (universal stress protein PHOS32 [Momordica charantia])

HSP 1 Score: 112.5 bits (280), Expect = 2.1e-21
Identity = 52/58 (89.66%), Postives = 56/58 (96.55%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          MVKDRK GVALDFSNSSKNAL+WAIDNLADKGDTL++IYVNPNSLDESAH LWA+SGS
Sbjct: 1  MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGS 58

[Close]

BLAST of Spg033229 vs. NCBI nr
Match: XP_022935543.1 (universal stress protein PHOS32-like [Cucurbita moschata])

HSP 1 Score: 107.5 bits (267), Expect = 6.6e-20
Identity = 50/59 (84.75%), Postives = 57/59 (96.61%), Query Frame = 0


Query: 35  EMVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
           +MVKDRKIGVALDFS+SSKNAL+WAIDNLADKGDTL+IIYVNPNS++ES H LWA+SGS
Sbjct: 49  KMVKDRKIGVALDFSSSSKNALRWAIDNLADKGDTLFIIYVNPNSVNESPHKLWAESGS 107

[Close]

BLAST of Spg033229 vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 6.9e-04
Identity = 19/41 (46.34%), Postives = 29/41 (70.73%), Query Frame = 0


Query: 40 RKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSL 81
          RKIGVA+D S  S  A++WA+D+    GD + I++V+P S+
Sbjct: 45 RKIGVAVDLSEESAFAVRWAVDHYIRPGDAVVILHVSPTSV 85

[Close]

BLAST of Spg033229 vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 9.0e-04
Identity = 18/41 (43.90%), Postives = 29/41 (70.73%), Query Frame = 0


Query: 40 RKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSL 81
          RKIGVA+D S  S  A++WA+D+    GD + +++V+P S+
Sbjct: 48 RKIGVAVDLSEESSFAVRWAVDHYIRPGDAVVLLHVSPTSV 88

[Close]

BLAST of Spg033229 vs. ExPASy TrEMBL
Match: A0A5A7TSD0 (Universal stress protein PHOS32-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001270 PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.9e-22
Identity = 54/58 (93.10%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          MVKDRKIGVALDFS+SSKNALKWAIDNLADKGDTLYIIYVNPNSL+ESAH LWA+SGS
Sbjct: 1  MVKDRKIGVALDFSDSSKNALKWAIDNLADKGDTLYIIYVNPNSLNESAHRLWAESGS 58

[Close]

BLAST of Spg033229 vs. ExPASy TrEMBL
Match: A0A1S3B5T0 (uncharacterized protein C167.05 OS=Cucumis melo OX=3656 GN=LOC103486344 PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.9e-22
Identity = 54/58 (93.10%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          MVKDRKIGVALDFS+SSKNALKWAIDNLADKGDTLYIIYVNPNSL+ESAH LWA+SGS
Sbjct: 1  MVKDRKIGVALDFSDSSKNALKWAIDNLADKGDTLYIIYVNPNSLNESAHRLWAESGS 58

[Close]

BLAST of Spg033229 vs. ExPASy TrEMBL
Match: A0A0A0LDK3 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G733270 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 7.6e-22
Identity = 53/58 (91.38%), Postives = 57/58 (98.28%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          MVKDRKIGVALDFSNSSKNAL+WAIDNLADKGDTL+IIYVNPNSL+ESAH LWA+SGS
Sbjct: 1  MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLEESAHRLWAESGS 58

[Close]

BLAST of Spg033229 vs. ExPASy TrEMBL
Match: A0A6J1CXB3 (universal stress protein PHOS32 OS=Momordica charantia OX=3673 GN=LOC111015049 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 1.0e-21
Identity = 52/58 (89.66%), Postives = 56/58 (96.55%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          MVKDRK GVALDFSNSSKNAL+WAIDNLADKGDTL++IYVNPNSLDESAH LWA+SGS
Sbjct: 1  MVKDRKFGVALDFSNSSKNALRWAIDNLADKGDTLFVIYVNPNSLDESAHQLWAESGS 58

[Close]

BLAST of Spg033229 vs. ExPASy TrEMBL
Match: A0A6J1FAU1 (universal stress protein PHOS32-like OS=Cucurbita moschata OX=3662 GN=LOC111442383 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 3.2e-20
Identity = 50/59 (84.75%), Postives = 57/59 (96.61%), Query Frame = 0


Query: 35  EMVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
           +MVKDRKIGVALDFS+SSKNAL+WAIDNLADKGDTL+IIYVNPNS++ES H LWA+SGS
Sbjct: 49  KMVKDRKIGVALDFSSSSKNALRWAIDNLADKGDTLFIIYVNPNSVNESPHKLWAESGS 107

[Close]

BLAST of Spg033229 vs. TAIR 10
Match: AT3G53990.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 85.9 bits (211), Expect = 1.9e-17
Identity = 41/58 (70.69%), Postives = 48/58 (82.76%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          M KDR IG+A+DFS SSKNALKWAI+NLADKGDT+YII+  P S DES + LW +SGS
Sbjct: 1  MPKDRNIGIAMDFSESSKNALKWAIENLADKGDTIYIIHTLPLSGDESRNSLWFKSGS 58

[Close]

BLAST of Spg033229 vs. TAIR 10
Match: AT3G53990.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 85.9 bits (211), Expect = 1.9e-17
Identity = 41/58 (70.69%), Postives = 48/58 (82.76%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          M KDR IG+A+DFS SSKNALKWAI+NLADKGDT+YII+  P S DES + LW +SGS
Sbjct: 1  MPKDRNIGIAMDFSESSKNALKWAIENLADKGDTIYIIHTLPLSGDESRNSLWFKSGS 58

[Close]

BLAST of Spg033229 vs. TAIR 10
Match: AT3G03270.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 2.1e-08
Identity = 25/58 (43.10%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          M K R +GV +D+S +SK AL+WA +NL + GDT+ +I+V P + D +   L+ ++GS
Sbjct: 1  MGKARTVGVGMDYSPTSKLALRWAAENLLEDGDTVILIHVQPQNADHTRKILFEETGS 58

[Close]

BLAST of Spg033229 vs. TAIR 10
Match: AT3G03270.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 2.1e-08
Identity = 25/58 (43.10%), Postives = 40/58 (68.97%), Query Frame = 0


Query: 36 MVKDRKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYVNPNSLDESAHHLWAQSGS 94
          M K R +GV +D+S +SK AL+WA +NL + GDT+ +I+V P + D +   L+ ++GS
Sbjct: 1  MGKARTVGVGMDYSPTSKLALRWAAENLLEDGDTVILIHVQPQNADHTRKILFEETGS 58

[Close]

BLAST of Spg033229 vs. TAIR 10
Match: AT3G17020.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 50.8 bits (120), Expect = 6.9e-07
Identity = 26/55 (47.27%), Postives = 35/55 (63.64%), Query Frame = 0


Query: 40 RKIGVALDFSNSSKNALKWAIDNLADKGDTLYIIYV-NPNSLDESAHHLWAQSGS 94
          R+IGVA+DFS+ SK AL WAIDN+   GD L +I + +  + +E    LW   GS
Sbjct: 7  RRIGVAVDFSDCSKKALSWAIDNVVRDGDHLILITIAHDMNYEEGEMQLWETVGS 61

The following BLAST results are available for this feature:

BLAST of Spg033229 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903504.1	5.4e-22	93.10	universal stress protein PHOS32 [Benincasa hispida]	[more]
XP_008442491.1	1.2e-21	93.10	PREDICTED: uncharacterized protein C167.05 [Cucumis melo] >KAA0044129.1 universa...	[more]
XP_011651876.1	1.6e-21	91.38	universal stress protein PHOS32 [Cucumis sativus] >KGN58822.1 hypothetical prote...	[more]
XP_022145652.1	2.1e-21	89.66	universal stress protein PHOS32 [Momordica charantia]	[more]
XP_022935543.1	6.6e-20	84.75	universal stress protein PHOS32-like [Cucurbita moschata]	[more]

BLAST of Spg033229 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L4N1	6.9e-04	46.34	Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S...	[more]
Q8VYN9	9.0e-04	43.90	Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S...	[more]

BLAST of Spg033229 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TSD0	5.9e-22	93.10	Universal stress protein PHOS32-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B5T0	5.9e-22	93.10	uncharacterized protein C167.05 OS=Cucumis melo OX=3656 GN=LOC103486344 PE=4 SV=...	[more]
A0A0A0LDK3	7.6e-22	91.38	Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G733270 PE=4 SV...	[more]
A0A6J1CXB3	1.0e-21	89.66	universal stress protein PHOS32 OS=Momordica charantia OX=3673 GN=LOC111015049 P...	[more]
A0A6J1FAU1	3.2e-20	84.75	universal stress protein PHOS32-like OS=Cucurbita moschata OX=3662 GN=LOC1114423...	[more]

BLAST of Spg033229 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53990.2	1.9e-17	70.69	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G53990.1	1.9e-17	70.69	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G03270.1	2.1e-08	43.10	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G03270.2	2.1e-08	43.10	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G17020.1	6.9e-07	47.27	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 16..94 e-value: 1.7E-8 score: 36.6
IPR006016	UspA	PFAM	PF00582	Usp	coord: 40..89 e-value: 1.8E-9 score: 38.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..38
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..31
None	No IPR available	PANTHER	PTHR46100:SF14	SUBFAMILY NOT NAMED	coord: 36..93
None	No IPR available	PANTHER	PTHR46100	IMP2'P	coord: 36..93
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 40..84

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg033229.1	Spg033229.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
biological_process	GO:0009409	response to cold
biological_process	GO:0009408	response to heat
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0034337	RNA folding
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding