Spg033186 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg033186 vs. NCBI nr
Match: XP_022983005.1 (protein CHROMOSOME TRANSMISSION FIDELITY 7 [Cucurbita maxima])

HSP 1 Score: 603.6 bits (1555), Expect = 1.1e-168
Identity = 299/364 (82.14%), Postives = 327/364 (89.84%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPEPLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSDR 60
           MQSKISAFFKP  +ISAHKSPEPLN+ A  + DDGALKIWE AEHQY+NTYKRRAPSSDR
Sbjct: 1   MQSKISAFFKPCPSISAHKSPEPLNTDADDS-DDGALKIWEKAEHQYVNTYKRRAPSSDR 60

Query: 61  NVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCPT 120
            V++PQCVD E  IPI  +  A  EL S TK VVKNKKRSYAQYHLLFGQSDFLLHFC T
Sbjct: 61  IVRSPQCVDSEINIPIPGDSPAKEELVSLTKPVVKNKKRSYAQYHLLFGQSDFLLHFCST 120

Query: 121 CGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHR 180
           CGIKYARGDEGDEK HK+FH+KYTCGI+FKGWSHER+I++ +VE GRILLVL+SDPSAH 
Sbjct: 121 CGIKYARGDEGDEKYHKSFHKKYTCGIQFKGWSHERLINISTVEDGRILLVLNSDPSAHW 180

Query: 181 NKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERPE 240
           NK+EEVVKMMEKELG+GWIL KN QVYLFVSSQRIVGCLV EPI KA+KVV CFLDERPE
Sbjct: 181 NKIEEVVKMMEKELGNGWILYKNCQVYLFVSSQRIVGCLVAEPIMKAYKVVSCFLDERPE 240

Query: 241 ETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRA 300
           E+KMKDSKPNSTTLQFG+I F+R+ ILKKPT+NPEA D+NLNG ILCEEEAVPAVCG+RA
Sbjct: 241 ESKMKDSKPNSTTLQFGNISFRREAILKKPTDNPEALDVNLNGVILCEEEAVPAVCGIRA 300

Query: 301 IWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVY 360
           IWVTPANRRKHIASQLLDAAR+SFCKG +LECSQLAFSQPTSSGMALASRYVGSRSIL Y
Sbjct: 301 IWVTPANRRKHIASQLLDAARKSFCKGGSLECSQLAFSQPTSSGMALASRYVGSRSILAY 360

Query: 361 KSSS 365
           KS +
Sbjct: 361 KSDT 363

BLAST of Spg033186 vs. NCBI nr
Match: XP_022151358.1 (protein CHROMOSOME TRANSMISSION FIDELITY 7 isoform X1 [Momordica charantia])

HSP 1 Score: 600.5 bits (1547), Expect = 9.6e-168
Identity = 304/365 (83.29%), Postives = 328/365 (89.86%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPE-PLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSD 60
           MQSKIS FFKPSS+ISAH+S E PLNS   AADDDGALKIWE AEHQY+NTYKRR  SS 
Sbjct: 1   MQSKISVFFKPSSSISAHRSTESPLNS---AADDDGALKIWEKAEHQYVNTYKRRTSSSH 60

Query: 61  RNVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCP 120
           RNV++P+ V    + PI D+ S N+E+ASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCP
Sbjct: 61  RNVRSPEYVIPAPKNPILDDSSVNLEVASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCP 120

Query: 121 TCGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAH 180
           TCGIKYARGDEGDEKSHK FH+ YTCGI+FKGWSHERVI+V S EGGRILLVLDSDPSAH
Sbjct: 121 TCGIKYARGDEGDEKSHKTFHKNYTCGIQFKGWSHERVINVTSDEGGRILLVLDSDPSAH 180

Query: 181 RNKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERP 240
           RNKVE VVKMMEK+LGSGWIL K+ QVYLFVSSQRIVGCLVVEPITKAFKVV CFLDE+P
Sbjct: 181 RNKVEAVVKMMEKDLGSGWILHKDCQVYLFVSSQRIVGCLVVEPITKAFKVVSCFLDEKP 240

Query: 241 EETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVR 300
           +E+KMKDS+ +STTLQFG I FQR+ ILKKPT NPEA D NLNGAILCEEEAVPAVCGVR
Sbjct: 241 DESKMKDSRQSSTTLQFGDITFQREAILKKPTKNPEALDKNLNGAILCEEEAVPAVCGVR 300

Query: 301 AIWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILV 360
           AIWVTPANRRKHIASQLLDAAR+SFCKG ALECSQLAFSQPTS GMALASRYVGSR ILV
Sbjct: 301 AIWVTPANRRKHIASQLLDAARKSFCKGVALECSQLAFSQPTSCGMALASRYVGSRFILV 360

Query: 361 YKSSS 365
           YKS++
Sbjct: 361 YKSNT 362

BLAST of Spg033186 vs. NCBI nr
Match: KAG6580745.1 (Protein CHROMOSOME TRANSMISSION FIDELITY 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-167
Identity = 298/364 (81.87%), Postives = 325/364 (89.29%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPEPLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSDR 60
           MQSKISAFFKP  +ISAHKSPEPLN+ A  + DDGALKIWE AEHQY+NTYKRRAPSSDR
Sbjct: 31  MQSKISAFFKPRPSISAHKSPEPLNTDADDS-DDGALKIWEKAEHQYVNTYKRRAPSSDR 90

Query: 61  NVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCPT 120
            VK+PQCVD E  IPI  +  A  EL S TK VVKNKKRSYAQYHLLFGQSDFLLHFC T
Sbjct: 91  IVKSPQCVDSEINIPIPGDSPAKDELVSLTKPVVKNKKRSYAQYHLLFGQSDFLLHFCST 150

Query: 121 CGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHR 180
           CGIKYARGDEGDEK HK+FH+KYTCGI+FKGWSHER+I + +VE GRILLVL+SDPSAH 
Sbjct: 151 CGIKYARGDEGDEKYHKSFHKKYTCGIQFKGWSHERLIHISTVEDGRILLVLNSDPSAHW 210

Query: 181 NKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERPE 240
           NK+EEVVKMMEKELG+GWIL KN QVYLFVSSQRIVGCLVVEPI KA+KVV CFLDERPE
Sbjct: 211 NKIEEVVKMMEKELGNGWILHKNCQVYLFVSSQRIVGCLVVEPIMKAYKVVSCFLDERPE 270

Query: 241 ETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRA 300
           E+KMKDSKPNSTTLQFG+I F+R+ ILKKPT+NPEA D+NLNG ILCEEEAVPAVCG+RA
Sbjct: 271 ESKMKDSKPNSTTLQFGNISFRREAILKKPTDNPEALDVNLNGVILCEEEAVPAVCGIRA 330

Query: 301 IWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVY 360
           IWVTPANRRKHIA+QLLDAAR+ FCK  +LECSQLAFSQPTSSGMALASRYVGSRSIL Y
Sbjct: 331 IWVTPANRRKHIATQLLDAARKRFCKEGSLECSQLAFSQPTSSGMALASRYVGSRSILAY 390

Query: 361 KSSS 365
           KS +
Sbjct: 391 KSDT 393

BLAST of Spg033186 vs. NCBI nr
Match: XP_023528008.1 (protein CHROMOSOME TRANSMISSION FIDELITY 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 599.4 bits (1544), Expect = 2.1e-167
Identity = 297/364 (81.59%), Postives = 325/364 (89.29%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPEPLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSDR 60
           MQSKISAFFKP  +ISAHKSPEPLN+ A    DDGALKIWE AEHQY+NTYKRRAPSSDR
Sbjct: 1   MQSKISAFFKPRPSISAHKSPEPLNTDADDC-DDGALKIWEKAEHQYVNTYKRRAPSSDR 60

Query: 61  NVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCPT 120
            V++PQCVD E  IPI  +  A  EL S TK VVKNKKRSYAQYHLLFGQSDFLLHFC T
Sbjct: 61  IVRSPQCVDSEINIPIPGDSPAKEELVSLTKPVVKNKKRSYAQYHLLFGQSDFLLHFCST 120

Query: 121 CGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHR 180
           CGIKYARGDEGDE  HK+FH+KYTCGI+FKGWSHER+I++ +VE GRILLVL+SDPSAH 
Sbjct: 121 CGIKYARGDEGDETYHKSFHKKYTCGIQFKGWSHERLINISTVEDGRILLVLNSDPSAHW 180

Query: 181 NKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERPE 240
           NK+EEVVKMMEKELG+GWIL KN QVYLFVSSQRIVGCLVVEPI KA+KVV CFLDERPE
Sbjct: 181 NKIEEVVKMMEKELGNGWILHKNCQVYLFVSSQRIVGCLVVEPIMKAYKVVSCFLDERPE 240

Query: 241 ETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRA 300
           E+KMKDSKPNSTTLQFG+I F+R+ ILKKPT+NPEA D+ LNG ILCEEEAVPAVCG+RA
Sbjct: 241 ESKMKDSKPNSTTLQFGNISFRREAILKKPTDNPEALDVTLNGVILCEEEAVPAVCGIRA 300

Query: 301 IWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVY 360
           IWVTPANRRKHIA+QLLDAAR+SFCKG +LECSQLAFSQPTSSGMALASRYVGSRSIL Y
Sbjct: 301 IWVTPANRRKHIATQLLDAARKSFCKGGSLECSQLAFSQPTSSGMALASRYVGSRSILAY 360

Query: 361 KSSS 365
           KS +
Sbjct: 361 KSDT 363

BLAST of Spg033186 vs. NCBI nr
Match: XP_022934206.1 (protein CHROMOSOME TRANSMISSION FIDELITY 7 [Cucurbita moschata])

HSP 1 Score: 598.6 bits (1542), Expect = 3.6e-167
Identity = 297/364 (81.59%), Postives = 324/364 (89.01%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPEPLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSDR 60
           MQSKISAFFKP  +ISAHKSPEPLN+ A  + DDGALKIWE AEHQY+N YKRRAPSSDR
Sbjct: 1   MQSKISAFFKPRPSISAHKSPEPLNTDADDS-DDGALKIWEKAEHQYVNAYKRRAPSSDR 60

Query: 61  NVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCPT 120
            VK+PQCVD E  IPI  +  A  EL S TK VVKNKKRSYAQYHLLFGQSDFLLHFC T
Sbjct: 61  IVKSPQCVDSEINIPIPGDSPAKDELVSLTKPVVKNKKRSYAQYHLLFGQSDFLLHFCST 120

Query: 121 CGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHR 180
           CGIKYARGDE DEK HK+FH+KYTCGI+FKGWSHER+I + +VE GRILLV +SDPSAH 
Sbjct: 121 CGIKYARGDEADEKYHKSFHKKYTCGIQFKGWSHERLIHISTVEDGRILLVFNSDPSAHW 180

Query: 181 NKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERPE 240
           NK+EEVVKMMEKELG+GWIL KN QVYLFVSSQRIVGCLVVEPI KA+KVV CFLDERPE
Sbjct: 181 NKIEEVVKMMEKELGNGWILHKNCQVYLFVSSQRIVGCLVVEPIMKAYKVVSCFLDERPE 240

Query: 241 ETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRA 300
           E+KMKDSKPNSTTLQFG+I F+R+ ILKKPT+NPEA D+NLNG ILCEEEAVPAVCG+RA
Sbjct: 241 ESKMKDSKPNSTTLQFGNISFRREAILKKPTDNPEALDVNLNGVILCEEEAVPAVCGIRA 300

Query: 301 IWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVY 360
           IWVTPANRRKHIA+QLLDAAR+SFCKG +LECSQLAFSQPTSSGMALASRYVGSRSIL Y
Sbjct: 301 IWVTPANRRKHIATQLLDAARKSFCKGGSLECSQLAFSQPTSSGMALASRYVGSRSILAY 360

Query: 361 KSSS 365
           KS +
Sbjct: 361 KSDT 363

BLAST of Spg033186 vs. ExPASy Swiss-Prot
Match: A7UL74 (Protein CHROMOSOME TRANSMISSION FIDELITY 7 OS=Arabidopsis thaliana OX=3702 GN=CTF7 PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-85
Identity = 175/365 (47.95%), Postives = 237/365 (64.93%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPEPLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSDR 60
           MQ+KI++FFKPSS+ S         +A+   D D  L +WEN  +  +NTY+RR+  ++R
Sbjct: 1   MQAKINSFFKPSSSSSI--------AASVTTDTDDGLAVWENNRNAIVNTYQRRSAITER 60

Query: 61  NVKTPQCVDLETRIPISDELSANVELASSTKTVVKN--KKRSYAQYHLLFGQSDFLLHFC 120
           +     C++                L   + +V KN  KKR+Y Q+HL  GQSDFLL  C
Sbjct: 61  SEVLKGCIE--------------KTLKKGSSSVPKNHKKKRNYTQFHLELGQSDFLLRHC 120

Query: 121 PTCGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSA 180
             CG KYA GDE DEK+H++FH+ Y  G+ FKGW +E+    P     RI++V ++D  A
Sbjct: 121 AECGAKYAPGDELDEKNHQSFHKDYMYGLPFKGWQNEKAFTSPLFIKNRIVMVSENDSPA 180

Query: 181 HRNKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDER 240
           HRNKV+EVVKMME ELG  WIL ++ +VYLF+SSQRI GCLV EPI +AFK++    DER
Sbjct: 181 HRNKVQEVVKMMEVELGEDWILHQHCKVYLFISSQRISGCLVAEPIKEAFKLIASPDDER 240

Query: 241 PEETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGV 300
             + + + S   ST++QFG+I+ QR+ + K+   + +  D   NG I+CEEEA PAVCG+
Sbjct: 241 --QLQKESSSSPSTSIQFGNIVLQRE-VSKRCRTSDDRLD---NGVIVCEEEAKPAVCGI 300

Query: 301 RAIWVTPANRRKHIASQLLDAARESFC-KGAALECSQLAFSQPTSSGMALASRYVGSRSI 360
           RAIWV+P+NRRK IA+ LLD  RESFC  G  LE SQLAFSQP+S G +  S+Y G+ S 
Sbjct: 301 RAIWVSPSNRRKGIATWLLDTTRESFCNNGCMLEKSQLAFSQPSSIGRSFGSKYFGTCSF 337

Query: 361 LVYKS 363
           L+YK+
Sbjct: 361 LLYKA 337

BLAST of Spg033186 vs. ExPASy Swiss-Prot
Match: Q56NI9 (N-acetyltransferase ESCO2 OS=Homo sapiens OX=9606 GN=ESCO2 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.5e-30
Identity = 90/286 (31.47%), Postives = 132/286 (46.15%), Query Frame = 0

Query: 83  NVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCPTCGIKYARGDEGDEKSHKAFHEK 142
           N++  ++T+   K  K    Q  +  GQ  F    C +CG+ Y   +  DE  H   H +
Sbjct: 357 NIQKNTNTRDTSKKTK---DQLIIDAGQKHFGATVCKSCGMIYTASNPEDEMQHVQHHHR 416

Query: 143 YTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHRNKVEEVVKMMEKELGSGWILQK 202
           +  GI++ GW  ERV  V     G+I+LVL  DPS    KVE+V ++++ ELG   ++ K
Sbjct: 417 FLEGIKYVGWKKERV--VAEFWDGKIVLVLPHDPSFAIKKVEDVQELVDNELGFQQVVPK 476

Query: 203 ---NYQVYLFVSSQ-RIVGCLVVEPITKAFKVVLCFLDERPEETKMKDSKPNSTTLQFGH 262
                + +LF+S + R+VGCL+ EPI +AF+V                            
Sbjct: 477 CPNKIKTFLFISDEKRVVGCLIAEPIKQAFRV---------------------------- 536

Query: 263 IIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRAIWVTPANRRKHIASQLLD 322
                   L +P      S      A  C +   PAVCG+  IWV    RRK IA +L+D
Sbjct: 537 --------LSEPIGPESPSSTECPRAWQCSDVPEPAVCGISRIWVFRLKRRKRIARRLVD 596

Query: 323 AARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVYKSSS 365
             R  F  G  L   ++AFS PT  G   A++Y  + + LVY  +S
Sbjct: 597 TLRNCFMFGCFLSTDEIAFSDPTPDGKLFATKYCNTPNFLVYNFNS 601

BLAST of Spg033186 vs. ExPASy Swiss-Prot
Match: Q8CIB9 (N-acetyltransferase ESCO2 OS=Mus musculus OX=10090 GN=Esco2 PE=1 SV=3)

HSP 1 Score: 132.1 bits (331), Expect = 1.3e-29
Identity = 83/255 (32.55%), Postives = 121/255 (47.45%), Query Frame = 0

Query: 109 GQSDFLLHFCPTCGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRI 168
           GQ  F    C +CG+ Y   +  DE  H   H ++  GI+F GW  ERV  V     G+I
Sbjct: 377 GQKHFGTTVCKSCGMIYTASNPEDEIQHLQHHHRFLEGIKFVGWKRERV--VAEFWDGKI 436

Query: 169 LLVLDSDPSAHRNKVEEVVKMMEKELG---SGWILQKNYQVYLFVSSQRIVGCLVVEPIT 228
           +LVL  DPS    KVE+V ++++ ELG   +  +     + +LF+  +R+VGCL+ EPI 
Sbjct: 437 VLVLPRDPSYAIKKVEDVQELVDLELGFQQTVPVCPDKTKTFLFIDEKRVVGCLIAEPIK 496

Query: 229 KAFKVVLCFLDERPEETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAI 288
           +AF+V                                    L +P+ + E S      A 
Sbjct: 497 QAFRV------------------------------------LSEPSASKECS-----RAW 556

Query: 289 LCEEEAVPAVCGVRAIWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGM 348
            C +   PA+CG+  IWV    RRK IA +L+D  R  F  G  L  +++AFS PT  G 
Sbjct: 557 RCSDVPEPAICGISRIWVFRLKRRKRIARRLVDTVRNCFMFGCFLSTNEIAFSDPTPDGK 588

Query: 349 ALASRYVGSRSILVY 361
             A++Y  + + LVY
Sbjct: 617 LFATKYCNTPNFLVY 588

BLAST of Spg033186 vs. ExPASy Swiss-Prot
Match: Q5FWF5 (N-acetyltransferase ESCO1 OS=Homo sapiens OX=9606 GN=ESCO1 PE=1 SV=3)

HSP 1 Score: 123.2 bits (308), Expect = 5.9e-27
Identity = 95/338 (28.11%), Postives = 155/338 (45.86%), Query Frame = 0

Query: 33  DDGALKIWENAEHQYINTYKRRAPSSD-----RNVKTPQC-VDLETRIPISDELSANVEL 92
           D G  K   +A  Q+     + + SSD     RN   P+C   LE  IP       +++L
Sbjct: 541 DTGENKFPGSAPQQHSILSNQTSKSSDNRETPRNHSLPKCNSHLEITIP------KDLKL 600

Query: 93  ASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCPTCGIKYARGDEGDEKSHKAFHEKYTCG 152
             + KT  K       Q  +  GQ  F    C  CG+ Y   +  DE  H  FH ++   
Sbjct: 601 KEAEKTDEK-------QLIIDAGQKRFGAVSCNVCGMLYTASNPEDETQHLLFHNQFISA 660

Query: 153 IRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHRNKVEEVVKMMEKELG---SGWILQKN 212
           +++ GW  ER++       GRI++VL  DP     KV+E+ +M++ +LG   +  +    
Sbjct: 661 VKYVGWKKERIL--AEYPDGRIIMVLPEDPKYALKKVDEIREMVDNDLGFQQAPLMCYSR 720

Query: 213 YQVYLFVSS-QRIVGCLVVEPITKAFKVVLCFLDERPEETKMKDSKPNSTTLQFGHIIFQ 272
            +  LF+S+ +++VGCL+ E I   ++V+    +E+    + ++ K          + F+
Sbjct: 721 TKTLLFISNDKKVVGCLIAEHIQWGYRVI----EEKLPVIRSEEEK----------VRFE 780

Query: 273 RKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRAIWVTPANRRKHIASQLLDAARE 332
           R+                   A  C     PA+CG+  IWV    RRK IAS++++  R 
Sbjct: 781 RQK------------------AWCCSTLPEPAICGISRIWVFSMMRRKKIASRMIECLRS 831

Query: 333 SFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVY 361
           +F  G+ L   ++AFS PT  G   A++Y G+   LVY
Sbjct: 841 NFIYGSYLSKEEIAFSDPTPDGKLFATQYCGTGQFLVY 831

BLAST of Spg033186 vs. ExPASy Swiss-Prot
Match: Q5SPR8 (N-acetyltransferase ESCO2 OS=Danio rerio OX=7955 GN=esco2 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 5.9e-27
Identity = 86/287 (29.97%), Postives = 137/287 (47.74%), Query Frame = 0

Query: 80  LSANVELASSTKTVVKNKKRSYAQYHLLF--GQSDFLLHFCPTCGIKYARGDEGDEKSHK 139
           L+A   L +  +++++ + +      L+   GQ  F    C +CG+ Y+     D   H 
Sbjct: 354 LTATPALKAKERSMLRREMKKQTDNQLIIDAGQKQFGATTCASCGMLYSTDSPEDNFQHT 413

Query: 140 AFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHRNKVEEVVKMMEKELGSG 199
            FH+++   I+F GW  ERV  V     G+I+LVL  DP     K E+V ++ + ELG  
Sbjct: 414 QFHQRFLDTIKFVGWKKERV--VAEFWDGKIILVLPDDPKYATRKAEDVRRIADSELGFQ 473

Query: 200 WIL---QKNYQVYLFVSSQR-IVGCLVVEPITKAFKVVLCFLDERPEETKMKDSKPNSTT 259
            I      + + YLF+++ R +VGCLV E I +A++V+        ++ K KD   +   
Sbjct: 474 QITLSSPSSAKTYLFINTDRMVVGCLVAENIRQAYRVL-------EQQEKQKDM--SKED 533

Query: 260 LQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRAIWVTPANRRKHIA 319
               H  +   T+ +K                        A+CGV  IWV    RRK +A
Sbjct: 534 FMEHHRTWCCSTVPEK------------------------ALCGVSRIWVFSLMRRKSVA 593

Query: 320 SQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVY 361
           ++LLD AR +F  G+ L   ++AFS PT  G   A++Y  + + LVY
Sbjct: 594 TRLLDTARNTFMYGSHLTKEEIAFSDPTPQGKLFATKYCQTPTFLVY 605

BLAST of Spg033186 vs. ExPASy TrEMBL
Match: A0A6J1J4G6 (protein CHROMOSOME TRANSMISSION FIDELITY 7 OS=Cucurbita maxima OX=3661 GN=LOC111481677 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.5e-169
Identity = 299/364 (82.14%), Postives = 327/364 (89.84%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPEPLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSDR 60
           MQSKISAFFKP  +ISAHKSPEPLN+ A  + DDGALKIWE AEHQY+NTYKRRAPSSDR
Sbjct: 1   MQSKISAFFKPCPSISAHKSPEPLNTDADDS-DDGALKIWEKAEHQYVNTYKRRAPSSDR 60

Query: 61  NVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCPT 120
            V++PQCVD E  IPI  +  A  EL S TK VVKNKKRSYAQYHLLFGQSDFLLHFC T
Sbjct: 61  IVRSPQCVDSEINIPIPGDSPAKEELVSLTKPVVKNKKRSYAQYHLLFGQSDFLLHFCST 120

Query: 121 CGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHR 180
           CGIKYARGDEGDEK HK+FH+KYTCGI+FKGWSHER+I++ +VE GRILLVL+SDPSAH 
Sbjct: 121 CGIKYARGDEGDEKYHKSFHKKYTCGIQFKGWSHERLINISTVEDGRILLVLNSDPSAHW 180

Query: 181 NKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERPE 240
           NK+EEVVKMMEKELG+GWIL KN QVYLFVSSQRIVGCLV EPI KA+KVV CFLDERPE
Sbjct: 181 NKIEEVVKMMEKELGNGWILYKNCQVYLFVSSQRIVGCLVAEPIMKAYKVVSCFLDERPE 240

Query: 241 ETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRA 300
           E+KMKDSKPNSTTLQFG+I F+R+ ILKKPT+NPEA D+NLNG ILCEEEAVPAVCG+RA
Sbjct: 241 ESKMKDSKPNSTTLQFGNISFRREAILKKPTDNPEALDVNLNGVILCEEEAVPAVCGIRA 300

Query: 301 IWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVY 360
           IWVTPANRRKHIASQLLDAAR+SFCKG +LECSQLAFSQPTSSGMALASRYVGSRSIL Y
Sbjct: 301 IWVTPANRRKHIASQLLDAARKSFCKGGSLECSQLAFSQPTSSGMALASRYVGSRSILAY 360

Query: 361 KSSS 365
           KS +
Sbjct: 361 KSDT 363

BLAST of Spg033186 vs. ExPASy TrEMBL
Match: A0A6J1DBZ2 (protein CHROMOSOME TRANSMISSION FIDELITY 7 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019321 PE=3 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 4.6e-168
Identity = 304/365 (83.29%), Postives = 328/365 (89.86%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPE-PLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSD 60
           MQSKIS FFKPSS+ISAH+S E PLNS   AADDDGALKIWE AEHQY+NTYKRR  SS 
Sbjct: 1   MQSKISVFFKPSSSISAHRSTESPLNS---AADDDGALKIWEKAEHQYVNTYKRRTSSSH 60

Query: 61  RNVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCP 120
           RNV++P+ V    + PI D+ S N+E+ASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCP
Sbjct: 61  RNVRSPEYVIPAPKNPILDDSSVNLEVASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCP 120

Query: 121 TCGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAH 180
           TCGIKYARGDEGDEKSHK FH+ YTCGI+FKGWSHERVI+V S EGGRILLVLDSDPSAH
Sbjct: 121 TCGIKYARGDEGDEKSHKTFHKNYTCGIQFKGWSHERVINVTSDEGGRILLVLDSDPSAH 180

Query: 181 RNKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERP 240
           RNKVE VVKMMEK+LGSGWIL K+ QVYLFVSSQRIVGCLVVEPITKAFKVV CFLDE+P
Sbjct: 181 RNKVEAVVKMMEKDLGSGWILHKDCQVYLFVSSQRIVGCLVVEPITKAFKVVSCFLDEKP 240

Query: 241 EETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVR 300
           +E+KMKDS+ +STTLQFG I FQR+ ILKKPT NPEA D NLNGAILCEEEAVPAVCGVR
Sbjct: 241 DESKMKDSRQSSTTLQFGDITFQREAILKKPTKNPEALDKNLNGAILCEEEAVPAVCGVR 300

Query: 301 AIWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILV 360
           AIWVTPANRRKHIASQLLDAAR+SFCKG ALECSQLAFSQPTS GMALASRYVGSR ILV
Sbjct: 301 AIWVTPANRRKHIASQLLDAARKSFCKGVALECSQLAFSQPTSCGMALASRYVGSRFILV 360

Query: 361 YKSSS 365
           YKS++
Sbjct: 361 YKSNT 362

BLAST of Spg033186 vs. ExPASy TrEMBL
Match: A0A6J1F175 (protein CHROMOSOME TRANSMISSION FIDELITY 7 OS=Cucurbita moschata OX=3662 GN=LOC111441447 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.8e-167
Identity = 297/364 (81.59%), Postives = 324/364 (89.01%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPEPLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSDR 60
           MQSKISAFFKP  +ISAHKSPEPLN+ A  + DDGALKIWE AEHQY+N YKRRAPSSDR
Sbjct: 1   MQSKISAFFKPRPSISAHKSPEPLNTDADDS-DDGALKIWEKAEHQYVNAYKRRAPSSDR 60

Query: 61  NVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCPT 120
            VK+PQCVD E  IPI  +  A  EL S TK VVKNKKRSYAQYHLLFGQSDFLLHFC T
Sbjct: 61  IVKSPQCVDSEINIPIPGDSPAKDELVSLTKPVVKNKKRSYAQYHLLFGQSDFLLHFCST 120

Query: 121 CGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHR 180
           CGIKYARGDE DEK HK+FH+KYTCGI+FKGWSHER+I + +VE GRILLV +SDPSAH 
Sbjct: 121 CGIKYARGDEADEKYHKSFHKKYTCGIQFKGWSHERLIHISTVEDGRILLVFNSDPSAHW 180

Query: 181 NKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERPE 240
           NK+EEVVKMMEKELG+GWIL KN QVYLFVSSQRIVGCLVVEPI KA+KVV CFLDERPE
Sbjct: 181 NKIEEVVKMMEKELGNGWILHKNCQVYLFVSSQRIVGCLVVEPIMKAYKVVSCFLDERPE 240

Query: 241 ETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRA 300
           E+KMKDSKPNSTTLQFG+I F+R+ ILKKPT+NPEA D+NLNG ILCEEEAVPAVCG+RA
Sbjct: 241 ESKMKDSKPNSTTLQFGNISFRREAILKKPTDNPEALDVNLNGVILCEEEAVPAVCGIRA 300

Query: 301 IWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVY 360
           IWVTPANRRKHIA+QLLDAAR+SFCKG +LECSQLAFSQPTSSGMALASRYVGSRSIL Y
Sbjct: 301 IWVTPANRRKHIATQLLDAARKSFCKGGSLECSQLAFSQPTSSGMALASRYVGSRSILAY 360

Query: 361 KSSS 365
           KS +
Sbjct: 361 KSDT 363

BLAST of Spg033186 vs. ExPASy TrEMBL
Match: A0A6J1DCV0 (protein CHROMOSOME TRANSMISSION FIDELITY 7 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019321 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.4e-164
Identity = 301/365 (82.47%), Postives = 324/365 (88.77%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPE-PLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSD 60
           MQSKIS FFKPSS+ISAH+S E PLNS   AADDDGALKIWE AEHQY+NTYKRR  SS 
Sbjct: 1   MQSKISVFFKPSSSISAHRSTESPLNS---AADDDGALKIWEKAEHQYVNTYKRRTSSS- 60

Query: 61  RNVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCP 120
               +P+ V    + PI D+ S N+E+ASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCP
Sbjct: 61  ---HSPEYVIPAPKNPILDDSSVNLEVASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCP 120

Query: 121 TCGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAH 180
           TCGIKYARGDEGDEKSHK FH+ YTCGI+FKGWSHERVI+V S EGGRILLVLDSDPSAH
Sbjct: 121 TCGIKYARGDEGDEKSHKTFHKNYTCGIQFKGWSHERVINVTSDEGGRILLVLDSDPSAH 180

Query: 181 RNKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERP 240
           RNKVE VVKMMEK+LGSGWIL K+ QVYLFVSSQRIVGCLVVEPITKAFKVV CFLDE+P
Sbjct: 181 RNKVEAVVKMMEKDLGSGWILHKDCQVYLFVSSQRIVGCLVVEPITKAFKVVSCFLDEKP 240

Query: 241 EETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVR 300
           +E+KMKDS+ +STTLQFG I FQR+ ILKKPT NPEA D NLNGAILCEEEAVPAVCGVR
Sbjct: 241 DESKMKDSRQSSTTLQFGDITFQREAILKKPTKNPEALDKNLNGAILCEEEAVPAVCGVR 300

Query: 301 AIWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILV 360
           AIWVTPANRRKHIASQLLDAAR+SFCKG ALECSQLAFSQPTS GMALASRYVGSR ILV
Sbjct: 301 AIWVTPANRRKHIASQLLDAARKSFCKGVALECSQLAFSQPTSCGMALASRYVGSRFILV 358

Query: 361 YKSSS 365
           YKS++
Sbjct: 361 YKSNT 358

BLAST of Spg033186 vs. ExPASy TrEMBL
Match: A0A5D3DNU9 (Protein CHROMOSOME TRANSMISSION FIDELITY 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004540 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.5e-163
Identity = 295/363 (81.27%), Postives = 320/363 (88.15%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPEPLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSDR 60
           MQSKI  FFKP+S+IS+++SPEPLNS A  + D G LKIWE AEHQYINTYKR      R
Sbjct: 21  MQSKIGTFFKPTSSISSYRSPEPLNSNADDS-DYGTLKIWEKAEHQYINTYKR------R 80

Query: 61  NVKTPQCVDLETRIPISDELSANVELASSTKTVVKNKKRSYAQYHLLFGQSDFLLHFCPT 120
           NVK+PQCVD ET+  + D+ S   ELA STK VVKNKKRSYAQYHLLFGQSDFLLHFC T
Sbjct: 81  NVKSPQCVDSETKTSVPDDSSGKSELALSTKIVVKNKKRSYAQYHLLFGQSDFLLHFCST 140

Query: 121 CGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSAHR 180
           CGIKYARGD+ DE+SHKAFH+KYTCGI+FKGW+ ERVID PSVEGGRILLVLDSDPSAH+
Sbjct: 141 CGIKYARGDQDDEQSHKAFHKKYTCGIQFKGWTRERVIDTPSVEGGRILLVLDSDPSAHK 200

Query: 181 NKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDERPE 240
           NKVEEVVKMMEKELGSGWIL KNYQ YLFV SQRIVGCLVVEPITKA+KVV C L ERPE
Sbjct: 201 NKVEEVVKMMEKELGSGWILHKNYQAYLFVLSQRIVGCLVVEPITKAYKVVSCHLHERPE 260

Query: 241 ETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGVRA 300
           E+KMKDS P+S+TLQFG+I F R+ ILKKPTNNPEA D N+NGAILCEEEAVPAVCGVRA
Sbjct: 261 ESKMKDSIPSSSTLQFGNITFHREAILKKPTNNPEALDTNMNGAILCEEEAVPAVCGVRA 320

Query: 301 IWVTPANRRKHIASQLLDAARESFCKGAALECSQLAFSQPTSSGMALASRYVGSRSILVY 360
           IWVTPANRRKH+ASQLLDAAR+SF KG ALECSQLAFSQPTSSGMALASRYVGSRSILVY
Sbjct: 321 IWVTPANRRKHVASQLLDAARQSFYKGVALECSQLAFSQPTSSGMALASRYVGSRSILVY 376

Query: 361 KSS 364
           KSS
Sbjct: 381 KSS 376

BLAST of Spg033186 vs. TAIR 10
Match: AT4G31400.1 (damaged DNA binding;DNA-directed DNA polymerases )

HSP 1 Score: 318.2 bits (814), Expect = 8.8e-87
Identity = 175/365 (47.95%), Postives = 237/365 (64.93%), Query Frame = 0

Query: 1   MQSKISAFFKPSSTISAHKSPEPLNSAAAAADDDGALKIWENAEHQYINTYKRRAPSSDR 60
           MQ+KI++FFKPSS+ S         +A+   D D  L +WEN  +  +NTY+RR+  ++R
Sbjct: 1   MQAKINSFFKPSSSSSI--------AASVTTDTDDGLAVWENNRNAIVNTYQRRSAITER 60

Query: 61  NVKTPQCVDLETRIPISDELSANVELASSTKTVVKN--KKRSYAQYHLLFGQSDFLLHFC 120
           +     C++                L   + +V KN  KKR+Y Q+HL  GQSDFLL  C
Sbjct: 61  SEVLKGCIE--------------KTLKKGSSSVPKNHKKKRNYTQFHLELGQSDFLLRHC 120

Query: 121 PTCGIKYARGDEGDEKSHKAFHEKYTCGIRFKGWSHERVIDVPSVEGGRILLVLDSDPSA 180
             CG KYA GDE DEK+H++FH+ Y  G+ FKGW +E+    P     RI++V ++D  A
Sbjct: 121 AECGAKYAPGDELDEKNHQSFHKDYMYGLPFKGWQNEKAFTSPLFIKNRIVMVSENDSPA 180

Query: 181 HRNKVEEVVKMMEKELGSGWILQKNYQVYLFVSSQRIVGCLVVEPITKAFKVVLCFLDER 240
           HRNKV+EVVKMME ELG  WIL ++ +VYLF+SSQRI GCLV EPI +AFK++    DER
Sbjct: 181 HRNKVQEVVKMMEVELGEDWILHQHCKVYLFISSQRISGCLVAEPIKEAFKLIASPDDER 240

Query: 241 PEETKMKDSKPNSTTLQFGHIIFQRKTILKKPTNNPEASDINLNGAILCEEEAVPAVCGV 300
             + + + S   ST++QFG+I+ QR+ + K+   + +  D   NG I+CEEEA PAVCG+
Sbjct: 241 --QLQKESSSSPSTSIQFGNIVLQRE-VSKRCRTSDDRLD---NGVIVCEEEAKPAVCGI 300

Query: 301 RAIWVTPANRRKHIASQLLDAARESFC-KGAALECSQLAFSQPTSSGMALASRYVGSRSI 360
           RAIWV+P+NRRK IA+ LLD  RESFC  G  LE SQLAFSQP+S G +  S+Y G+ S 
Sbjct: 301 RAIWVSPSNRRKGIATWLLDTTRESFCNNGCMLEKSQLAFSQPSSIGRSFGSKYFGTCSF 337

Query: 361 LVYKS 363
           L+YK+
Sbjct: 361 LLYKA 337