Spg033176 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg033176
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionLaccase
Locationscaffold5: 1430080 .. 1433010 (-)
RNA-Seq ExpressionSpg033176
SyntenySpg033176
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAAATCTAAACCTTTTAATTACATGGAGAGAAATACTAAAAGGTCTGAATTATTACTCGTGATAGAAATATACAACTCCAAAGGGTTGGAGAGAAAAATAAAATAAAATTAAAAATTAAAAATTAAAAACAGAAACTTTATGTAACATGTAAGATATAATAAAAGAAAACGGTGCATCTGAAAATTCTTTTGAATGAAGAATCGTTTTAAGTTGTCACCTAACTGAGAAAAAAACGACATGGCAACGACACAGAGCTAGCTTCTTTCCATTCAAACTCATTTGCTGTTCCTCTTCTTTATAAGGTCGACTTCAATGGCTATCCTTCTCATCATCCTCCTCATTTCTAAAACATCAAAATACACACACAAGGAGGAAGATTGATGGAGTCTTCGTGGGTTCGAGCTTTTCTCGTGTTGGCATTTTGCTGCCTTCTTCCTGCTCTCGCCGAGTCCAGAGTTCGCCATTATAAGTTTAATGTGAGCGTTTTCTGATAACCAATTGGTTTTCTGTTTTCTTGAAGACCAAGTCATTTTTAAACACTGTTTTTTCAAGATAAAGAGATATAACAATATATATATCAAGCTTTCTAATTCTTCACTCTCTATATCTTTTATCATCTCTATTTTCTTGCTCTAACATCTCTCCTCAAACTTTTTAGAAAATAGAAAACAGTTATCAAATATGCTTTTTCTTAATCAGAAAAACAAAGGGTTTCCAATGTTGATCACAGCTTCGTAGCTAGCTTTCTTTTTTTTTAGAAAGAAAGCTTTAATTTTGGATGACCTTTTTCTGTTATGGGTGGGCCTCCAATGTTGGTTTGGTTTGATTTTGAAGAGTGATTTTGAGCCATTTTGGTTGGCAGGTGGTGTTGAGGAAGGCCACCAGACTTTGCTCAAGCAAGCCCATTGTCACCGTTAATGGACTGTTCCCTGGACCAACCCTTTACGCTCGGGAGGACGACACTGTTCTCATCAAAGTCGTCAACCATGTCAAATATAACCTTTCCATTCACTGGTAAATCCCACAAAATCTACTCTCTGTAATGTAATGGTGACAGAAATTGCATAATCTCATGAATACAATGTTGGTGGTAGGCATGGAATCAGGCAACTTCGAACTGGCTGGGCCGATGGACCGGCATACATTACACAATGTCCAATTCAACCCGGGCAAAGCTACGTGTACAACTTCACCATCACCGGCCAAAGAGGCACGCTCTTCTGGCATGCTCATATTCTCTGGCTGAGAGCCACTGTCCATGGCGCCATCGTCATCTTGCCGAAGCTCGGCGTGCCTTATCCATTCCCTACACCCCATAAAGAAACTGTCCTTGTGCTTGGTATGAGCCTCGAAAGTTAACTACTTTTGCTTTTCCCCTTTTAGAAAAGTGGAGATTTTGATTTGCTTTTGTGAATGAATGGTGATTTGTATTTTATAGCTGAGTGGTGGAAGTCAGATACCGAAGCTGTGATCAACGAAGCTCTCAAGTCAGGATTAGCACCAAATGTCTCTGATGCTCACACCATCAACGGCCTTTCAGGATCCATATCAAATTGCCCTTCACAAAGTAATTCAATCAAGAAACTAAACTAATTTGAACTTTTCTGATGGCTCGTCTAATGTTCTTCCTTTTGGTAATCAGGGGGCTTCACATTGCCTGTTCAAAAAGGGAAGACCTATTTACTCCGCATAATCAACGCTGCACTTAATGAAGAACTGTTTTTCAAAGTCGCTGGCCACAAAATAACAGTAGTAGAAGTCGACGCCACATATGTCAAACCATTCAAAACAGACGTAGTTCTAGTAGCTCCCGGCCAGACAACCAACGTCCTCTTAACCGCAGATAAAAACTCTGGCAAATACATGGTGGCGGTCTCTCCATTCATGGACGCTCCAGTCGCCGTTGATAACAAGACCGCCACTGCCACCGTGCACTACACCGGCACATTGGCCACATCCCTCACCACAAGCACCAACCCCCCTCCCCAAAACGCAACACAAATCGCCAATACCTTCACAAACTCCCTCAGAAGCTTGAACTCCAAAAAGTACCCTGCCAAAGTCCCATTGTCAATCGACCACCATCTCTTCTTCACTGTAGGGCTTGGAATCAACCCATGTCCAACTTGCAAGGCCGCCAATGGAAGCAGGGTAGTGGCCAGTATCAACAATGTCACTTTTGCAATGCCCACAACCGCTCTTCTACAAGCACATTTCTTCAAAATCAATGGCGTTTTCACCACAGATTTTCCCGGAAACCCCCCTCACAAATTCAACTACACAGGACCCGGACCTAAAAATTTGCAGACGACAAGTGGAACCAAGGTCTACAAATTACAATACAATTCCACAGTGGAATTAGTTCTACAAGATACAGGAATCATAACCCCAGAAAACCATCCCATTCATCTCCACGGATTCAATTTCTTCGAAGTCGGGCGAGGAATCGGCAATTTCAACTCTAAAACCGACCCCAAGAAATTCAATCTCGTGGATCCGGTGGAGAGAAACACAATCGGAGTTCCCTCCGGTGGATGGACGGCGATAAGGTTTCGTGCGGACAATCCAGGTAACAGAATTCGAATCCTCTTTCGATTTCTCGATCAAATCTTAAACTATCGAACTGAATTTAAACGATTGAATTGAAAACGAAACTGAAATTGAAGGGTTTTGATTTGATTTCACCTTGCAGGAGTTTGGTTTATGCATTGCCATTTGGAAATTCATACGACTTGGGGATTGAAGATGGCGTTCTTGGTGGAAAATGGCAAAGGCCCCAAACAGTCGCTCCTTCCGCCGCCGAGCGACCTCCCAAAATGTTAATCCAAAGGAAAAAAAAATAATAATAATAATAAAGGAATTGAAATTTTGTTTTTATACACATGGTGTAAAAATTGAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT

mRNA sequence

ATGGAGTCTTCGTGGGTTCGAGCTTTTCTCGTGTTGGCATTTTGCTGCCTTCTTCCTGCTCTCGCCGAGTCCAGAGTTCGCCATTATAAGTTTAATGTGGTGTTGAGGAAGGCCACCAGACTTTGCTCAAGCAAGCCCATTGTCACCGTTAATGGACTGTTCCCTGGACCAACCCTTTACGCTCGGGAGGACGACACTGTTCTCATCAAAGTCGTCAACCATGTCAAATATAACCTTTCCATTCACTGGCATGGAATCAGGCAACTTCGAACTGGCTGGGCCGATGGACCGGCATACATTACACAATGTCCAATTCAACCCGGGCAAAGCTACGTGTACAACTTCACCATCACCGGCCAAAGAGGCACGCTCTTCTGGCATGCTCATATTCTCTGGCTGAGAGCCACTGTCCATGGCGCCATCGTCATCTTGCCGAAGCTCGGCGTGCCTTATCCATTCCCTACACCCCATAAAGAAACTGTCCTTGTGCTTGCTGAGTGGTGGAAGTCAGATACCGAAGCTGTGATCAACGAAGCTCTCAAGTCAGGATTAGCACCAAATGTCTCTGATGCTCACACCATCAACGGCCTTTCAGGATCCATATCAAATTGCCCTTCACAAAGGGGCTTCACATTGCCTGTTCAAAAAGGGAAGACCTATTTACTCCGCATAATCAACGCTGCACTTAATGAAGAACTGTTTTTCAAAGTCGCTGGCCACAAAATAACAGTAGTAGAAGTCGACGCCACATATGTCAAACCATTCAAAACAGACGTAGTTCTAGTAGCTCCCGGCCAGACAACCAACGTCCTCTTAACCGCAGATAAAAACTCTGGCAAATACATGGTGGCGGTCTCTCCATTCATGGACGCTCCAGTCGCCGTTGATAACAAGACCGCCACTGCCACCGTGCACTACACCGGCACATTGGCCACATCCCTCACCACAAGCACCAACCCCCCTCCCCAAAACGCAACACAAATCGCCAATACCTTCACAAACTCCCTCAGAAGCTTGAACTCCAAAAAGTACCCTGCCAAAGTCCCATTGTCAATCGACCACCATCTCTTCTTCACTGTAGGGCTTGGAATCAACCCATGTCCAACTTGCAAGGCCGCCAATGGAAGCAGGGTAGTGGCCAGTATCAACAATGTCACTTTTGCAATGCCCACAACCGCTCTTCTACAAGCACATTTCTTCAAAATCAATGGCGTTTTCACCACAGATTTTCCCGGAAACCCCCCTCACAAATTCAACTACACAGGACCCGGACCTAAAAATTTGCAGACGACAAGTGGAACCAAGGTCTACAAATTACAATACAATTCCACAGTGGAATTAGTTCTACAAGATACAGGAATCATAACCCCAGAAAACCATCCCATTCATCTCCACGGATTCAATTTCTTCGAAGTCGGGCGAGGAATCGGCAATTTCAACTCTAAAACCGACCCCAAGAAATTCAATCTCGTGGATCCGGTGGAGAGAAACACAATCGGAGTTCCCTCCGGTGGATGGACGGCGATAAGGTTTCGTGCGGACAATCCAGGAGTTTGGTTTATGCATTGCCATTTGGAAATTCATACGACTTGGGGATTGAAGATGGCGTTCTTGGTGGAAAATGGCAAAGGCCCCAAACAGTCGCTCCTTCCGCCGCCGAGCGACCTCCCAAAATGTTAA

Coding sequence (CDS)

ATGGAGTCTTCGTGGGTTCGAGCTTTTCTCGTGTTGGCATTTTGCTGCCTTCTTCCTGCTCTCGCCGAGTCCAGAGTTCGCCATTATAAGTTTAATGTGGTGTTGAGGAAGGCCACCAGACTTTGCTCAAGCAAGCCCATTGTCACCGTTAATGGACTGTTCCCTGGACCAACCCTTTACGCTCGGGAGGACGACACTGTTCTCATCAAAGTCGTCAACCATGTCAAATATAACCTTTCCATTCACTGGCATGGAATCAGGCAACTTCGAACTGGCTGGGCCGATGGACCGGCATACATTACACAATGTCCAATTCAACCCGGGCAAAGCTACGTGTACAACTTCACCATCACCGGCCAAAGAGGCACGCTCTTCTGGCATGCTCATATTCTCTGGCTGAGAGCCACTGTCCATGGCGCCATCGTCATCTTGCCGAAGCTCGGCGTGCCTTATCCATTCCCTACACCCCATAAAGAAACTGTCCTTGTGCTTGCTGAGTGGTGGAAGTCAGATACCGAAGCTGTGATCAACGAAGCTCTCAAGTCAGGATTAGCACCAAATGTCTCTGATGCTCACACCATCAACGGCCTTTCAGGATCCATATCAAATTGCCCTTCACAAAGGGGCTTCACATTGCCTGTTCAAAAAGGGAAGACCTATTTACTCCGCATAATCAACGCTGCACTTAATGAAGAACTGTTTTTCAAAGTCGCTGGCCACAAAATAACAGTAGTAGAAGTCGACGCCACATATGTCAAACCATTCAAAACAGACGTAGTTCTAGTAGCTCCCGGCCAGACAACCAACGTCCTCTTAACCGCAGATAAAAACTCTGGCAAATACATGGTGGCGGTCTCTCCATTCATGGACGCTCCAGTCGCCGTTGATAACAAGACCGCCACTGCCACCGTGCACTACACCGGCACATTGGCCACATCCCTCACCACAAGCACCAACCCCCCTCCCCAAAACGCAACACAAATCGCCAATACCTTCACAAACTCCCTCAGAAGCTTGAACTCCAAAAAGTACCCTGCCAAAGTCCCATTGTCAATCGACCACCATCTCTTCTTCACTGTAGGGCTTGGAATCAACCCATGTCCAACTTGCAAGGCCGCCAATGGAAGCAGGGTAGTGGCCAGTATCAACAATGTCACTTTTGCAATGCCCACAACCGCTCTTCTACAAGCACATTTCTTCAAAATCAATGGCGTTTTCACCACAGATTTTCCCGGAAACCCCCCTCACAAATTCAACTACACAGGACCCGGACCTAAAAATTTGCAGACGACAAGTGGAACCAAGGTCTACAAATTACAATACAATTCCACAGTGGAATTAGTTCTACAAGATACAGGAATCATAACCCCAGAAAACCATCCCATTCATCTCCACGGATTCAATTTCTTCGAAGTCGGGCGAGGAATCGGCAATTTCAACTCTAAAACCGACCCCAAGAAATTCAATCTCGTGGATCCGGTGGAGAGAAACACAATCGGAGTTCCCTCCGGTGGATGGACGGCGATAAGGTTTCGTGCGGACAATCCAGGAGTTTGGTTTATGCATTGCCATTTGGAAATTCATACGACTTGGGGATTGAAGATGGCGTTCTTGGTGGAAAATGGCAAAGGCCCCAAACAGTCGCTCCTTCCGCCGCCGAGCGACCTCCCAAAATGTTAA

Protein sequence

MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLYAREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQRGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEALKSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGHKITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTATATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTVGLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNYTGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFNSKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVENGKGPKQSLLPPPSDLPKC
Homology
BLAST of Spg033176 vs. NCBI nr
Match: XP_038904173.1 (laccase-4-like [Benincasa hispida])

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 529/559 (94.63%), Postives = 545/559 (97.50%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           MES WVRAF++LAFCCLLP LAES VRHYKFNVVLRKATRLCSSKPIVTVNG FPGPT+Y
Sbjct: 28  MESWWVRAFVLLAFCCLLPGLAESTVRHYKFNVVLRKATRLCSSKPIVTVNGQFPGPTIY 87

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           ARE D VL+KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQ+YVYNFTITGQ
Sbjct: 88  AREGDNVLVKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQNYVYNFTITGQ 147

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTLFWHAHILWLR+TVHGAIVILPKLGVPYPFPTP+KETVLVL EWWKSDTEAVINEAL
Sbjct: 148 RGTLFWHAHILWLRSTVHGAIVILPKLGVPYPFPTPNKETVLVLGEWWKSDTEAVINEAL 207

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSD+HTINGL GSISNCPSQRGFTLPVQ+GKTYLLRIINAALNEELFFKVAGH
Sbjct: 208 KSGLAPNVSDSHTINGLPGSISNCPSQRGFTLPVQRGKTYLLRIINAALNEELFFKVAGH 267

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
           K+TVVEVDATYVKPFKTD VL+APGQTTNVLLTA++NSGKYMVAVSPFMDAPVAVDNKTA
Sbjct: 268 KLTVVEVDATYVKPFKTDTVLIAPGQTTNVLLTANQNSGKYMVAVSPFMDAPVAVDNKTA 327

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
           TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPA VPL+IDHHLFFTV
Sbjct: 328 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPANVPLTIDHHLFFTV 387

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLGINPCPTCKAANGSRVVASINNVTF MPTTALLQAHFFK  GVFTTDFPGNPPHKFNY
Sbjct: 388 GLGINPCPTCKAANGSRVVASINNVTFVMPTTALLQAHFFKTKGVFTTDFPGNPPHKFNY 447

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           +GPGPKNLQTTSGTK+YKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN
Sbjct: 448 SGPGPKNLQTTSGTKLYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 507

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
            KTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE
Sbjct: 508 PKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 567

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGPKQSLLPPPSDLPKC
Sbjct: 568 NGKGPKQSLLPPPSDLPKC 586

BLAST of Spg033176 vs. NCBI nr
Match: XP_023539306.1 (laccase-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 527/559 (94.28%), Postives = 542/559 (96.96%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           MESS  R F++ A CCLLPALAES VRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY
Sbjct: 1   MESS--RCFVLFALCCLLPALAESTVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDTVL+KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ
Sbjct: 61  AREDDTVLVKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL
Sbjct: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSDAHTINGLSGS+SNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH
Sbjct: 181 KSGLAPNVSDAHTINGLSGSMSNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
           K+TVVEVDATYVKPFKTD VLVAPGQTTNVLLT DKNSGKYMVAVSPFMDAP+AVDNKTA
Sbjct: 241 KLTVVEVDATYVKPFKTDTVLVAPGQTTNVLLTTDKNSGKYMVAVSPFMDAPIAVDNKTA 300

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
           TATVHYTGT+A+SLTTSTNP PQNATQIAN FTN+LRSLNSKKYPA VPL+IDHHL FTV
Sbjct: 301 TATVHYTGTMASSLTTSTNPTPQNATQIANAFTNALRSLNSKKYPANVPLTIDHHLLFTV 360

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLGINPCPTCKA NGSRVVASINNVTF MPTTALLQAHFFKINGVFTTDFPG PPHKFNY
Sbjct: 361 GLGINPCPTCKAGNGSRVVASINNVTFTMPTTALLQAHFFKINGVFTTDFPGKPPHKFNY 420

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           TGPGPKNLQTTSGTK+YKL+YNSTVELVLQDTGI+TPENHPIHLHGFNFFEVGRG+GNFN
Sbjct: 421 TGPGPKNLQTTSGTKLYKLKYNSTVELVLQDTGIVTPENHPIHLHGFNFFEVGRGVGNFN 480

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
            KTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAF+VE
Sbjct: 481 PKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFMVE 540

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGPK SLLPPPSDLPKC
Sbjct: 541 NGKGPKHSLLPPPSDLPKC 557

BLAST of Spg033176 vs. NCBI nr
Match: XP_022937901.1 (laccase-4-like [Cucurbita moschata])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 527/559 (94.28%), Postives = 542/559 (96.96%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           MESS  R F++ A CCLLPALAES VRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY
Sbjct: 21  MESS--RCFVLFALCCLLPALAESTVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 80

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDTVL+KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTIT Q
Sbjct: 81  AREDDTVLVKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITAQ 140

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL
Sbjct: 141 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 200

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH
Sbjct: 201 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 260

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
           ++TVVEVDATYVKPFKTD VLVAPGQTTNVLLT DKNSGKYMVAVSPFMDAP+AVDNKTA
Sbjct: 261 ELTVVEVDATYVKPFKTDTVLVAPGQTTNVLLTTDKNSGKYMVAVSPFMDAPIAVDNKTA 320

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
           TATVHYTGT+A+SLTTSTNPPPQNATQIAN FTN+LRSLNSKKYPA VPL+IDHHL FTV
Sbjct: 321 TATVHYTGTMASSLTTSTNPPPQNATQIANAFTNALRSLNSKKYPANVPLTIDHHLLFTV 380

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLGINPCPTCKA NGSRVVASINNVTF MPTTALLQAHFFKINGVFTTDFPG PPHKFNY
Sbjct: 381 GLGINPCPTCKAGNGSRVVASINNVTFTMPTTALLQAHFFKINGVFTTDFPGKPPHKFNY 440

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           TGPGPKNLQTTSGTK+YKL+YNSTVELVLQDTGI+TPENHPIHLHGFNFFEVGRG+GNFN
Sbjct: 441 TGPGPKNLQTTSGTKLYKLKYNSTVELVLQDTGIVTPENHPIHLHGFNFFEVGRGVGNFN 500

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
            KTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAF+VE
Sbjct: 501 PKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFMVE 560

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGPKQSL PPPSDLPKC
Sbjct: 561 NGKGPKQSLPPPPSDLPKC 577

BLAST of Spg033176 vs. NCBI nr
Match: XP_022971405.1 (laccase-4-like [Cucurbita maxima])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 527/559 (94.28%), Postives = 542/559 (96.96%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           MESS  R F++ A CCLLPALAES VRHYKFNVVLRKATRLCS+KPIVTVNGLFPGPTLY
Sbjct: 41  MESS--RCFVLFALCCLLPALAESTVRHYKFNVVLRKATRLCSNKPIVTVNGLFPGPTLY 100

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDTVL+KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ
Sbjct: 101 AREDDTVLVKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 160

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVI EAL
Sbjct: 161 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVIKEAL 220

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH
Sbjct: 221 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 280

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
           K+TVVEVDATYVKPFKTD VLVAPGQTTNVLLT DKNSGKYMVAVSPFMDAP+AVDNKTA
Sbjct: 281 KLTVVEVDATYVKPFKTDTVLVAPGQTTNVLLTTDKNSGKYMVAVSPFMDAPIAVDNKTA 340

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
           TATVHYTGT+A+SLTTSTNPPPQNATQIAN FTN+LRSLNSKKYPA VPL+IDHHL FTV
Sbjct: 341 TATVHYTGTMASSLTTSTNPPPQNATQIANAFTNALRSLNSKKYPANVPLTIDHHLLFTV 400

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLGINPCPTCKA NGSRVVASINNVTF MPTTALLQAHFFKINGVFTTDFPG PPHKFNY
Sbjct: 401 GLGINPCPTCKAGNGSRVVASINNVTFTMPTTALLQAHFFKINGVFTTDFPGKPPHKFNY 460

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           TG GPKNLQTTSGTK+YKL+YNSTVELVLQDTGI+TPENHPIHLHGFNFFEVGRG+GNFN
Sbjct: 461 TGAGPKNLQTTSGTKLYKLKYNSTVELVLQDTGIVTPENHPIHLHGFNFFEVGRGVGNFN 520

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
            KTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAF+VE
Sbjct: 521 PKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFMVE 580

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGPKQSLLPPPSDLPKC
Sbjct: 581 NGKGPKQSLLPPPSDLPKC 597

BLAST of Spg033176 vs. NCBI nr
Match: XP_022145678.1 (laccase-4-like [Momordica charantia])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 526/560 (93.93%), Postives = 543/560 (96.96%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           MES WVRAF++LA CCLLP LAESRVRHYKFNVVLRKATRLCSSKPIVTVNG FPGPTLY
Sbjct: 1   MESLWVRAFILLAICCLLPHLAESRVRHYKFNVVLRKATRLCSSKPIVTVNGQFPGPTLY 60

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDTVL+KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTI GQ
Sbjct: 61  AREDDTVLVKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTIAGQ 120

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTL WHAHILWLRATVHGAIVILPKLGVPYPFPTPHKE VLVLAEWWKSDTEAVINEAL
Sbjct: 121 RGTLLWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKEAVLVLAEWWKSDTEAVINEAL 180

Query: 181 KSGLAPNVSDAHTINGLSGSIS-NCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAG 240
           KSGLAPNVSDAHTINGL GSIS NCPSQRGFTLPVQ GKTYLLRIINAALNEELFFKVAG
Sbjct: 181 KSGLAPNVSDAHTINGLPGSISTNCPSQRGFTLPVQSGKTYLLRIINAALNEELFFKVAG 240

Query: 241 HKITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKT 300
           HK+TVVEVDATYVKPFKTD VL+APGQTTNVLLTAD+NSGKYMVAVSPFMD+PVAVDN T
Sbjct: 241 HKLTVVEVDATYVKPFKTDTVLIAPGQTTNVLLTADRNSGKYMVAVSPFMDSPVAVDNNT 300

Query: 301 ATATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFT 360
           ATATVHY+GTLATSLTTSTNPPPQNATQIAN+FTNSLRSLNSKKYPA VPL+IDHHLFFT
Sbjct: 301 ATATVHYSGTLATSLTTSTNPPPQNATQIANSFTNSLRSLNSKKYPANVPLTIDHHLFFT 360

Query: 361 VGLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFN 420
           VGLGINPCPTCKAANGSRVVASINNVTF MPTTALLQAHFFKI+GVFTTDFPGNPPHKFN
Sbjct: 361 VGLGINPCPTCKAANGSRVVASINNVTFVMPTTALLQAHFFKISGVFTTDFPGNPPHKFN 420

Query: 421 YTGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNF 480
           Y+GPGP+NLQTTSGTK+YKLQYNSTVELVLQDTGIIT ENHP+HLHGFNFFEVGRGIGNF
Sbjct: 421 YSGPGPRNLQTTSGTKLYKLQYNSTVELVLQDTGIITAENHPVHLHGFNFFEVGRGIGNF 480

Query: 481 NSKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLV 540
           N KTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLE+HTTWGLKMAFLV
Sbjct: 481 NPKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEVHTTWGLKMAFLV 540

Query: 541 ENGKGPKQSLLPPPSDLPKC 560
           +NGKGPKQSL PPPSDLPKC
Sbjct: 541 DNGKGPKQSLPPPPSDLPKC 560

BLAST of Spg033176 vs. ExPASy Swiss-Prot
Match: O80434 (Laccase-4 OS=Arabidopsis thaliana OX=3702 GN=IRX12 PE=2 SV=2)

HSP 1 Score: 930.2 bits (2403), Expect = 1.1e-269
Identity = 435/559 (77.82%), Postives = 485/559 (86.76%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           M S  V    +++F  + PA +ES VRHYKFNVV++  TRLCSSKP VTVNG +PGPT+Y
Sbjct: 1   MGSHMVWFLFLVSFFSVFPAPSESMVRHYKFNVVMKNVTRLCSSKPTVTVNGRYPGPTIY 60

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDT+LIKVVNHVKYN+SIHWHG+RQ+RTGWADGPAYITQCPIQPGQ Y YN+T+TGQ
Sbjct: 61  AREDDTLLIKVVNHVKYNVSIHWHGVRQVRTGWADGPAYITQCPIQPGQVYTYNYTLTGQ 120

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTL+WHAHILWLRATV+GA+VILPK GVPYPFP P  E V+VL EWWKSDTE +INEAL
Sbjct: 121 RGTLWWHAHILWLRATVYGALVILPKRGVPYPFPKPDNEKVIVLGEWWKSDTENIINEAL 180

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSD+H ING  G + NCPSQ G+ L V+ GKTYLLR++NAALNEELFFKVAGH
Sbjct: 181 KSGLAPNVSDSHMINGHPGPVRNCPSQ-GYKLSVENGKTYLLRLVNAALNEELFFKVAGH 240

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
             TVVEVDA YVKPFKTD VL+APGQTTNVLLTA K++GKY+V  SPFMDAP+AVDN TA
Sbjct: 241 IFTVVEVDAVYVKPFKTDTVLIAPGQTTNVLLTASKSAGKYLVTASPFMDAPIAVDNVTA 300

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
           TATVHY+GTL++S T  T PPPQNAT IAN FTNSLRSLNSKKYPA VP +IDHHLFFTV
Sbjct: 301 TATVHYSGTLSSSPTILTLPPPQNATSIANNFTNSLRSLNSKKYPALVPTTIDHHLFFTV 360

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLG+N CPTCKA NGSRVVASINNVTF MP TALL AH+F  +GVFTTDFP NPPH FNY
Sbjct: 361 GLGLNACPTCKAGNGSRVVASINNVTFIMPKTALLPAHYFNTSGVFTTDFPKNPPHVFNY 420

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           +G    N+ T +GT++YKL YN+TV+LVLQDTG+I PENHP+HLHGFNFFEVGRG+GNFN
Sbjct: 421 SGGSVTNMATETGTRLYKLPYNATVQLVLQDTGVIAPENHPVHLHGFNFFEVGRGLGNFN 480

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
           S  DPK FNLVDPVERNTIGVPSGGW  IRFRADNPGVWFMHCHLE+HTTWGLKMAFLVE
Sbjct: 481 STKDPKNFNLVDPVERNTIGVPSGGWVVIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVE 540

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGP QS+LPPP DLPKC
Sbjct: 541 NGKGPNQSILPPPKDLPKC 558

BLAST of Spg033176 vs. ExPASy Swiss-Prot
Match: Q6ID18 (Laccase-10 OS=Arabidopsis thaliana OX=3702 GN=LAC10 PE=2 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 6.9e-253
Identity = 403/551 (73.14%), Postives = 464/551 (84.21%), Query Frame = 0

Query: 10  LVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLYAREDDTVLI 69
           LVL      PA     +R Y FNVV ++ TR+CS+K IVTVNG FPGPT+YA EDDT+L+
Sbjct: 8   LVLFALLAFPACVHGAIRKYTFNVVTKQVTRICSTKQIVTVNGKFPGPTIYANEDDTILV 67

Query: 70  KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQRGTLFWHAH 129
            VVN+VKYN+SIHWHGIRQLRTGWADGPAYITQCPI+PG SYVYNFT+TGQRGTL+WHAH
Sbjct: 68  NVVNNVKYNVSIHWHGIRQLRTGWADGPAYITQCPIKPGHSYVYNFTVTGQRGTLWWHAH 127

Query: 130 ILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEALKSGLAPNVS 189
           +LWLRATVHGAIVILPKLG+PYPFP PH+E V++L EWWKSDTE V+NEALKSGLAPNVS
Sbjct: 128 VLWLRATVHGAIVILPKLGLPYPFPKPHREEVIILGEWWKSDTETVVNEALKSGLAPNVS 187

Query: 190 DAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGHKITVVEVDA 249
           DAH ING  G + NCPSQ  F L V+ GKTY+LR+INAALNEELFFK+AGH+ TVVEVDA
Sbjct: 188 DAHVINGHPGFVPNCPSQGNFKLAVESGKTYMLRLINAALNEELFFKIAGHRFTVVEVDA 247

Query: 250 TYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMD-APVAVDNKTATATVHYTG 309
            YVKPF TD +L+APGQTT  L++A + SG+Y++A +PF D A VAVDN+TATATVHY+G
Sbjct: 248 VYVKPFNTDTILIAPGQTTTALVSAARPSGQYLIAAAPFQDSAVVAVDNRTATATVHYSG 307

Query: 310 TLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTVGLGINPCP 369
           TL+ + T +T+PPPQNAT +ANTF NSLRSLNSK YPA VP+++DH L FTVGLGIN C 
Sbjct: 308 TLSATPTKTTSPPPQNATSVANTFVNSLRSLNSKTYPANVPITVDHDLLFTVGLGINRCH 367

Query: 370 TCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNYTGPGPKNL 429
           +CKA N SRVVA+INN+TF MP TALLQAH+F + G++TTDFP  P   F++TG  P NL
Sbjct: 368 SCKAGNFSRVVAAINNITFKMPKTALLQAHYFNLTGIYTTDFPAKPRRVFDFTGKPPSNL 427

Query: 430 QTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFNSKTDPKKF 489
            T   TK+YKL YNSTV++VLQDTG + PENHPIHLHGFNFF VG G GN+NSK D  KF
Sbjct: 428 ATMKATKLYKLPYNSTVQVVLQDTGNVAPENHPIHLHGFNFFVVGLGTGNYNSKKDSNKF 487

Query: 490 NLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVENGKGPKQS 549
           NLVDPVERNT+GVPSGGW AIRFRADNPGVWFMHCHLE+HTTWGLKMAFLVENGKGP QS
Sbjct: 488 NLVDPVERNTVGVPSGGWAAIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVENGKGPNQS 547

Query: 550 LLPPPSDLPKC 560
           + PPPSDLPKC
Sbjct: 548 IRPPPSDLPKC 558

BLAST of Spg033176 vs. ExPASy Swiss-Prot
Match: Q0IQU1 (Laccase-22 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC22 PE=2 SV=2)

HSP 1 Score: 854.0 bits (2205), Expect = 9.7e-247
Identity = 389/556 (69.96%), Postives = 473/556 (85.07%), Query Frame = 0

Query: 8   AFLVLAFCCLLPAL-AESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLYAREDDT 67
           + L++A C LL AL A +  RHYKFNVV+R  TRLCS+KPI+TVNG FPGPTLYARE D 
Sbjct: 11  SLLLMAACFLLQALSAHAITRHYKFNVVMRNMTRLCSTKPILTVNGKFPGPTLYAREGDN 70

Query: 68  VLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQRGTLFW 127
           VL+KVVNHV +N++IHWHG+RQ+RTGW DGPAYITQCPIQPG S++YNFTITGQRGTL W
Sbjct: 71  VLVKVVNHVAHNVTIHWHGVRQIRTGWYDGPAYITQCPIQPGSSFLYNFTITGQRGTLLW 130

Query: 128 HAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEALKSGLAP 187
           HAHI WLRATVHGAIVILPKLGVPYPFP PHKE V+VL EWWK DTE VIN+A++ G+ P
Sbjct: 131 HAHINWLRATVHGAIVILPKLGVPYPFPAPHKEAVIVLGEWWKEDTETVINQAMQLGVGP 190

Query: 188 NVSDAHTINGLSGSISNC-PSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGHKITVV 247
           N+SD+HTING  G +S C  SQ GF L V+ GKTY+LRIINAALN++LFFKVAGH++TVV
Sbjct: 191 NISDSHTINGHPGPLSECASSQDGFKLSVENGKTYMLRIINAALNDDLFFKVAGHELTVV 250

Query: 248 EVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTATATVH 307
           EVDA Y KPFKTD +L+ PGQTTNVL+ A++ +G+Y+++VSPFMDAPV VDNKT TAT+H
Sbjct: 251 EVDAVYTKPFKTDTLLITPGQTTNVLVRANQGAGRYLLSVSPFMDAPVQVDNKTGTATLH 310

Query: 308 YTGTLATSLTTST--NPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTVGLG 367
           Y  T+++S+ + T   PPPQNAT I + FT+SL SLNSK+YPA VP ++DH L  TVG+G
Sbjct: 311 YANTVSSSMASLTLVKPPPQNATHIVSKFTDSLHSLNSKEYPANVPQTVDHSLLLTVGVG 370

Query: 368 INPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNYTGP 427
           +NPCP+C   NG+RVV +INNVTF MP+T +LQAH++ I GVFT DFP  P HKFNYTG 
Sbjct: 371 VNPCPSC--INGTRVVGTINNVTFIMPSTPILQAHYYNIPGVFTEDFPATPLHKFNYTGS 430

Query: 428 GPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFNSKT 487
           GPKNLQT +GT+VY+L YN++V++VLQDTGII+PE+HPIHLHGFNFF VG+G+GN+N +T
Sbjct: 431 GPKNLQTMNGTRVYRLPYNASVQVVLQDTGIISPESHPIHLHGFNFFVVGKGVGNYNPRT 490

Query: 488 DPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVENGK 547
            P  FNL+DP+ERNTIGVP+GGWTAIRFR+DNPGVWFMHCH E+HT+WGLKMAF+V+NGK
Sbjct: 491 SPSTFNLIDPIERNTIGVPTGGWTAIRFRSDNPGVWFMHCHFEVHTSWGLKMAFVVDNGK 550

Query: 548 GPKQSLLPPPSDLPKC 560
            P ++L+PPP DLP+C
Sbjct: 551 RPSETLIPPPKDLPQC 564

BLAST of Spg033176 vs. ExPASy Swiss-Prot
Match: Q1PDH6 (Laccase-16 OS=Arabidopsis thaliana OX=3702 GN=LAC16 PE=2 SV=2)

HSP 1 Score: 827.0 bits (2135), Expect = 1.3e-238
Identity = 396/553 (71.61%), Postives = 456/553 (82.46%), Query Frame = 0

Query: 14  FCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLYAREDDTVLIKVVN 73
           F  L P    S +RHYKFN V+   T+LCSSKPIVTVNG FPGPT+ ARE DT+LIKVVN
Sbjct: 17  FVLLSPTTVHSIIRHYKFN-VMTNTTKLCSSKPIVTVNGQFPGPTIVAREGDTILIKVVN 76

Query: 74  HVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQRGTLFWHAHILWL 133
           HVKYN+SIHWHGIRQLRTGWADGPAYITQCPIQPGQ+Y++NFT+TGQRGTL+WHAHILWL
Sbjct: 77  HVKYNVSIHWHGIRQLRTGWADGPAYITQCPIQPGQNYLHNFTLTGQRGTLWWHAHILWL 136

Query: 134 RATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEALKSGLAPNVSDAHT 193
           RATVHGAIVILPKLGVPYPFP P+KE  +VL+EWWKSD E +INEA + G AP+ SDAHT
Sbjct: 137 RATVHGAIVILPKLGVPYPFPKPYKEKTIVLSEWWKSDVEELINEASRIGTAPSASDAHT 196

Query: 194 INGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGHKITVVEVDATYVK 253
           ING SGSISNCPSQ  + LPV+ GKTY+LRIINAALNEELFFK+AGH +TVVEVDA Y K
Sbjct: 197 INGHSGSISNCPSQSSYGLPVRAGKTYMLRIINAALNEELFFKIAGHVLTVVEVDAVYTK 256

Query: 254 PFKTDVVLVAPGQTTNVLLTADKNSG-KYMVAVSPFMDAPVAVDNKTATATVHY---TGT 313
           P+KTD V +APGQTTNVLLTA+ N+G  YMVA + F DA +  DN TATAT+HY   T T
Sbjct: 257 PYKTDTVFIAPGQTTNVLLTANANAGSNYMVAATTFTDAHIPYDNVTATATLHYIGHTST 316

Query: 314 LATS-LTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTVGLGINPCP 373
           ++TS  T   + PPQNAT +A  FT SLRSLNS +YPA+VP +++H LFFTVGLG NPC 
Sbjct: 317 VSTSKKTVLASLPPQNATWVATKFTRSLRSLNSLEYPARVPTTVEHSLFFTVGLGANPCQ 376

Query: 374 TCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNYTGPGP--K 433
           +C   NG R+VA INNVTF MP TALLQAHFF I+GVFT DFP  P + ++YT P     
Sbjct: 377 SCN--NGVRLVAGINNVTFTMPKTALLQAHFFNISGVFTDDFPAKPSNPYDYTAPVKLGV 436

Query: 434 NLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFNSKTDPK 493
           N  T  GTK+Y+L YN+TV++VLQ+T +I  +NHP HLHGFNFFEVGRG+GNFN + DPK
Sbjct: 437 NAATMKGTKLYRLPYNATVQIVLQNTAMILSDNHPFHLHGFNFFEVGRGLGNFNPEKDPK 496

Query: 494 KFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVENGKGPK 553
            FNLVDPVERNT+GVP+GGWTAIRF ADNPGVWFMHCHLE+HTTWGLKMAF+V+NG GP 
Sbjct: 497 AFNLVDPVERNTVGVPAGGWTAIRFIADNPGVWFMHCHLELHTTWGLKMAFVVDNGHGPD 556

Query: 554 QSLLPPPSDLPKC 560
           QSLLPPP+DLPKC
Sbjct: 557 QSLLPPPADLPKC 566

BLAST of Spg033176 vs. ExPASy Swiss-Prot
Match: Q8VZA1 (Laccase-11 OS=Arabidopsis thaliana OX=3702 GN=LAC11 PE=2 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 3.6e-209
Identity = 338/556 (60.79%), Postives = 423/556 (76.08%), Query Frame = 0

Query: 9   FLVLAFCCLLPAL----AESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLYARED 68
           FL L FC LL  L     ++ V+ Y+F+V ++  +R+C++KPIVTVNG+FPGPT+YARE 
Sbjct: 5   FLFL-FCYLLAFLGYSPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYAREG 64

Query: 69  DTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQRGTL 128
           D V+I V NHV+YN+SIHWHG++Q R GWADGPAYITQCPIQ GQSY+Y+F +TGQRGTL
Sbjct: 65  DRVIINVTNHVQYNMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVTGQRGTL 124

Query: 129 FWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEALKSGL 188
           +WHAHILWLRATV+GAIVILP  G PYPFP P++E+ ++L EWW  D E  +N+A + G 
Sbjct: 125 WWHAHILWLRATVYGAIVILPAPGKPYPFPQPYQESNIILGEWWNKDVETAVNQANQLGA 184

Query: 189 APNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGHKITV 248
            P +SDAHTING  G +  C  +  F +  + GKTYLLRIINAALN+ELFF +AGH +TV
Sbjct: 185 PPPMSDAHTINGKPGPLFPCSEKHTFVIEAEAGKTYLLRIINAALNDELFFGIAGHNMTV 244

Query: 249 VEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTATATV 308
           VE+DA Y KPF T  +L+ PGQTTNVL+  D++  +Y +A SPFMDAPV+VDNKT TA +
Sbjct: 245 VEIDAVYTKPFTTKAILLGPGQTTNVLVKTDRSPNRYFMAASPFMDAPVSVDNKTVTAIL 304

Query: 309 HYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTVGLGI 368
            Y G   T L      P  N T  A  +   L+SLN+  +PA VPL +D  LF+T+GLGI
Sbjct: 305 QYKGVPNTVLPILPKLPLPNDTSFALDYNGKLKSLNTPNFPALVPLKVDRRLFYTIGLGI 364

Query: 369 NPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNYTG-P 428
           N CPTC   NG+ + ASINN+TF MP TALL+AH+  I+GVF TDFP  PP  FNYTG P
Sbjct: 365 NACPTC--VNGTNLAASINNITFIMPKTALLKAHYSNISGVFRTDFPDRPPKAFNYTGVP 424

Query: 429 GPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFNSKT 488
              NL T++GT++ ++++N+T+ELVLQDT ++T E+HP HLHG+NFF VG G+GNF+ K 
Sbjct: 425 LTANLGTSTGTRLSRVKFNTTIELVLQDTNLLTVESHPFHLHGYNFFVVGTGVGNFDPKK 484

Query: 489 DPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVENGK 548
           DP KFNLVDP ERNT+GVP+GGW AIRFRADNPGVWFMHCHLE+HT WGLKMAF+VENG+
Sbjct: 485 DPAKFNLVDPPERNTVGVPTGGWAAIRFRADNPGVWFMHCHLEVHTMWGLKMAFVVENGE 544

Query: 549 GPKQSLLPPPSDLPKC 560
            P+ S+LPPP D P C
Sbjct: 545 TPELSVLPPPKDYPSC 557

BLAST of Spg033176 vs. ExPASy TrEMBL
Match: A0A6J1I5M9 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111470137 PE=3 SV=1)

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 527/559 (94.28%), Postives = 542/559 (96.96%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           MESS  R F++ A CCLLPALAES VRHYKFNVVLRKATRLCS+KPIVTVNGLFPGPTLY
Sbjct: 41  MESS--RCFVLFALCCLLPALAESTVRHYKFNVVLRKATRLCSNKPIVTVNGLFPGPTLY 100

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDTVL+KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ
Sbjct: 101 AREDDTVLVKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 160

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVI EAL
Sbjct: 161 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVIKEAL 220

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH
Sbjct: 221 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 280

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
           K+TVVEVDATYVKPFKTD VLVAPGQTTNVLLT DKNSGKYMVAVSPFMDAP+AVDNKTA
Sbjct: 281 KLTVVEVDATYVKPFKTDTVLVAPGQTTNVLLTTDKNSGKYMVAVSPFMDAPIAVDNKTA 340

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
           TATVHYTGT+A+SLTTSTNPPPQNATQIAN FTN+LRSLNSKKYPA VPL+IDHHL FTV
Sbjct: 341 TATVHYTGTMASSLTTSTNPPPQNATQIANAFTNALRSLNSKKYPANVPLTIDHHLLFTV 400

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLGINPCPTCKA NGSRVVASINNVTF MPTTALLQAHFFKINGVFTTDFPG PPHKFNY
Sbjct: 401 GLGINPCPTCKAGNGSRVVASINNVTFTMPTTALLQAHFFKINGVFTTDFPGKPPHKFNY 460

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           TG GPKNLQTTSGTK+YKL+YNSTVELVLQDTGI+TPENHPIHLHGFNFFEVGRG+GNFN
Sbjct: 461 TGAGPKNLQTTSGTKLYKLKYNSTVELVLQDTGIVTPENHPIHLHGFNFFEVGRGVGNFN 520

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
            KTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAF+VE
Sbjct: 521 PKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFMVE 580

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGPKQSLLPPPSDLPKC
Sbjct: 581 NGKGPKQSLLPPPSDLPKC 597

BLAST of Spg033176 vs. ExPASy TrEMBL
Match: A0A6J1FBN4 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111444151 PE=3 SV=1)

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 527/559 (94.28%), Postives = 542/559 (96.96%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           MESS  R F++ A CCLLPALAES VRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY
Sbjct: 21  MESS--RCFVLFALCCLLPALAESTVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 80

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDTVL+KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTIT Q
Sbjct: 81  AREDDTVLVKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITAQ 140

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL
Sbjct: 141 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 200

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH
Sbjct: 201 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 260

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
           ++TVVEVDATYVKPFKTD VLVAPGQTTNVLLT DKNSGKYMVAVSPFMDAP+AVDNKTA
Sbjct: 261 ELTVVEVDATYVKPFKTDTVLVAPGQTTNVLLTTDKNSGKYMVAVSPFMDAPIAVDNKTA 320

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
           TATVHYTGT+A+SLTTSTNPPPQNATQIAN FTN+LRSLNSKKYPA VPL+IDHHL FTV
Sbjct: 321 TATVHYTGTMASSLTTSTNPPPQNATQIANAFTNALRSLNSKKYPANVPLTIDHHLLFTV 380

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLGINPCPTCKA NGSRVVASINNVTF MPTTALLQAHFFKINGVFTTDFPG PPHKFNY
Sbjct: 381 GLGINPCPTCKAGNGSRVVASINNVTFTMPTTALLQAHFFKINGVFTTDFPGKPPHKFNY 440

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           TGPGPKNLQTTSGTK+YKL+YNSTVELVLQDTGI+TPENHPIHLHGFNFFEVGRG+GNFN
Sbjct: 441 TGPGPKNLQTTSGTKLYKLKYNSTVELVLQDTGIVTPENHPIHLHGFNFFEVGRGVGNFN 500

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
            KTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAF+VE
Sbjct: 501 PKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFMVE 560

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGPKQSL PPPSDLPKC
Sbjct: 561 NGKGPKQSLPPPPSDLPKC 577

BLAST of Spg033176 vs. ExPASy TrEMBL
Match: A0A6J1CWL9 (Laccase OS=Momordica charantia OX=3673 GN=LOC111015070 PE=3 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 526/560 (93.93%), Postives = 543/560 (96.96%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           MES WVRAF++LA CCLLP LAESRVRHYKFNVVLRKATRLCSSKPIVTVNG FPGPTLY
Sbjct: 1   MESLWVRAFILLAICCLLPHLAESRVRHYKFNVVLRKATRLCSSKPIVTVNGQFPGPTLY 60

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDTVL+KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTI GQ
Sbjct: 61  AREDDTVLVKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTIAGQ 120

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTL WHAHILWLRATVHGAIVILPKLGVPYPFPTPHKE VLVLAEWWKSDTEAVINEAL
Sbjct: 121 RGTLLWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKEAVLVLAEWWKSDTEAVINEAL 180

Query: 181 KSGLAPNVSDAHTINGLSGSIS-NCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAG 240
           KSGLAPNVSDAHTINGL GSIS NCPSQRGFTLPVQ GKTYLLRIINAALNEELFFKVAG
Sbjct: 181 KSGLAPNVSDAHTINGLPGSISTNCPSQRGFTLPVQSGKTYLLRIINAALNEELFFKVAG 240

Query: 241 HKITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKT 300
           HK+TVVEVDATYVKPFKTD VL+APGQTTNVLLTAD+NSGKYMVAVSPFMD+PVAVDN T
Sbjct: 241 HKLTVVEVDATYVKPFKTDTVLIAPGQTTNVLLTADRNSGKYMVAVSPFMDSPVAVDNNT 300

Query: 301 ATATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFT 360
           ATATVHY+GTLATSLTTSTNPPPQNATQIAN+FTNSLRSLNSKKYPA VPL+IDHHLFFT
Sbjct: 301 ATATVHYSGTLATSLTTSTNPPPQNATQIANSFTNSLRSLNSKKYPANVPLTIDHHLFFT 360

Query: 361 VGLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFN 420
           VGLGINPCPTCKAANGSRVVASINNVTF MPTTALLQAHFFKI+GVFTTDFPGNPPHKFN
Sbjct: 361 VGLGINPCPTCKAANGSRVVASINNVTFVMPTTALLQAHFFKISGVFTTDFPGNPPHKFN 420

Query: 421 YTGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNF 480
           Y+GPGP+NLQTTSGTK+YKLQYNSTVELVLQDTGIIT ENHP+HLHGFNFFEVGRGIGNF
Sbjct: 421 YSGPGPRNLQTTSGTKLYKLQYNSTVELVLQDTGIITAENHPVHLHGFNFFEVGRGIGNF 480

Query: 481 NSKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLV 540
           N KTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLE+HTTWGLKMAFLV
Sbjct: 481 NPKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEVHTTWGLKMAFLV 540

Query: 541 ENGKGPKQSLLPPPSDLPKC 560
           +NGKGPKQSL PPPSDLPKC
Sbjct: 541 DNGKGPKQSLPPPPSDLPKC 560

BLAST of Spg033176 vs. ExPASy TrEMBL
Match: A0A6J1J7E9 (Laccase OS=Cucurbita maxima OX=3661 GN=LOC111481928 PE=3 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 519/559 (92.84%), Postives = 538/559 (96.24%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           MES  V AFL+LAFCCLLP LAESRVRHYK NVVLRKATRLCS+KPIVTVNG FPGPTLY
Sbjct: 1   MESWRVPAFLLLAFCCLLPGLAESRVRHYKLNVVLRKATRLCSTKPIVTVNGQFPGPTLY 60

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDTVL+KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ
Sbjct: 61  AREDDTVLVKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTLFWHAHILWLRATVHG IVILPKLGVPYPFPTPHKE VLVLAEWWKSDTEAVINEAL
Sbjct: 121 RGTLFWHAHILWLRATVHGGIVILPKLGVPYPFPTPHKEAVLVLAEWWKSDTEAVINEAL 180

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSDAHTINGL GSISNCP+QRGFTLPVQ+GKTYLLRIINAALNEELFFKVAGH
Sbjct: 181 KSGLAPNVSDAHTINGLPGSISNCPTQRGFTLPVQRGKTYLLRIINAALNEELFFKVAGH 240

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
           K+TVVEVDATYVKPFKTD VL+ PGQTTNVLLTAD+ SGKYMVA+SPFMDAP+AVDNKT 
Sbjct: 241 KLTVVEVDATYVKPFKTDTVLITPGQTTNVLLTADRKSGKYMVAISPFMDAPIAVDNKTG 300

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
            ATVHYTGTLATSLTT+TNPPPQNATQIANTFTNSLRSLNSKKYPAKVPL+IDHHL FTV
Sbjct: 301 IATVHYTGTLATSLTTNTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLTIDHHLLFTV 360

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLGINPCPTCKAANGSRVVASINN+TF MPTTALLQAHFFK  GVF+TDFPGNPPHKFNY
Sbjct: 361 GLGINPCPTCKAANGSRVVASINNITFVMPTTALLQAHFFKTKGVFSTDFPGNPPHKFNY 420

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           +GPGPKNLQTTSGTK+YKLQYNSTVELVLQDTGI+TPENHPIHLHGFNFFEVGRGIGNFN
Sbjct: 421 SGPGPKNLQTTSGTKLYKLQYNSTVELVLQDTGIVTPENHPIHLHGFNFFEVGRGIGNFN 480

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
            KTD KKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLE+HTTWGLKMAFLVE
Sbjct: 481 PKTDAKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVE 540

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGP+QSLLPPP DLPKC
Sbjct: 541 NGKGPQQSLLPPPRDLPKC 559

BLAST of Spg033176 vs. ExPASy TrEMBL
Match: A0A5D3DMZ0 (Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001020 PE=3 SV=1)

HSP 1 Score: 1092.8 bits (2825), Expect = 0.0e+00
Identity = 523/559 (93.56%), Postives = 537/559 (96.06%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           M SS VRAF++LAFCCLLP LAES VRHYKFNVVLRKATRLCSSKPIVTVNG FPGPTLY
Sbjct: 1   MGSSGVRAFVLLAFCCLLPGLAESTVRHYKFNVVLRKATRLCSSKPIVTVNGQFPGPTLY 60

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           ARE D VLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ
Sbjct: 61  AREGDNVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTLFWHAHILWLRATVHG +VILPKLGVPYPFPTP+KETVLVLAEWWKSDTEAVINEAL
Sbjct: 121 RGTLFWHAHILWLRATVHGGLVILPKLGVPYPFPTPNKETVLVLAEWWKSDTEAVINEAL 180

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSDAHTINGL GSI NCPSQRGFTLPVQ+GKTYLLRIINAALNEELFFKVAGH
Sbjct: 181 KSGLAPNVSDAHTINGLPGSIPNCPSQRGFTLPVQRGKTYLLRIINAALNEELFFKVAGH 240

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
           K+TVVEVDATYVKPFKTD V++APGQTTNVLLTA++NSGKYMVAVSPFMDAPVAVDNKTA
Sbjct: 241 KLTVVEVDATYVKPFKTDTVIIAPGQTTNVLLTANQNSGKYMVAVSPFMDAPVAVDNKTA 300

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
           TATVHYTGTLATSLTTSTNPPPQNATQIAN F NSLRSLNSKKYPA VPL+IDHHLFFTV
Sbjct: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANAFINSLRSLNSKKYPANVPLTIDHHLFFTV 360

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLGINPCPTCKAANGSRVVASINNVTF MPTTALLQAHFFK  GVFTTDFPGNPPHKFNY
Sbjct: 361 GLGINPCPTCKAANGSRVVASINNVTFVMPTTALLQAHFFKTKGVFTTDFPGNPPHKFNY 420

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           +GPGPKNLQTT+ TK+YKLQYNSTVELVLQDTGII PENHPIHLHGFNFFEVGRGIGNFN
Sbjct: 421 SGPGPKNLQTTTATKLYKLQYNSTVELVLQDTGIIAPENHPIHLHGFNFFEVGRGIGNFN 480

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
            KTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE
Sbjct: 481 PKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGP QSLLPPP DLPKC
Sbjct: 541 NGKGPNQSLLPPPRDLPKC 559

BLAST of Spg033176 vs. TAIR 10
Match: AT2G38080.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 930.2 bits (2403), Expect = 7.5e-271
Identity = 435/559 (77.82%), Postives = 485/559 (86.76%), Query Frame = 0

Query: 1   MESSWVRAFLVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLY 60
           M S  V    +++F  + PA +ES VRHYKFNVV++  TRLCSSKP VTVNG +PGPT+Y
Sbjct: 1   MGSHMVWFLFLVSFFSVFPAPSESMVRHYKFNVVMKNVTRLCSSKPTVTVNGRYPGPTIY 60

Query: 61  AREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQ 120
           AREDDT+LIKVVNHVKYN+SIHWHG+RQ+RTGWADGPAYITQCPIQPGQ Y YN+T+TGQ
Sbjct: 61  AREDDTLLIKVVNHVKYNVSIHWHGVRQVRTGWADGPAYITQCPIQPGQVYTYNYTLTGQ 120

Query: 121 RGTLFWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEAL 180
           RGTL+WHAHILWLRATV+GA+VILPK GVPYPFP P  E V+VL EWWKSDTE +INEAL
Sbjct: 121 RGTLWWHAHILWLRATVYGALVILPKRGVPYPFPKPDNEKVIVLGEWWKSDTENIINEAL 180

Query: 181 KSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGH 240
           KSGLAPNVSD+H ING  G + NCPSQ G+ L V+ GKTYLLR++NAALNEELFFKVAGH
Sbjct: 181 KSGLAPNVSDSHMINGHPGPVRNCPSQ-GYKLSVENGKTYLLRLVNAALNEELFFKVAGH 240

Query: 241 KITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTA 300
             TVVEVDA YVKPFKTD VL+APGQTTNVLLTA K++GKY+V  SPFMDAP+AVDN TA
Sbjct: 241 IFTVVEVDAVYVKPFKTDTVLIAPGQTTNVLLTASKSAGKYLVTASPFMDAPIAVDNVTA 300

Query: 301 TATVHYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTV 360
           TATVHY+GTL++S T  T PPPQNAT IAN FTNSLRSLNSKKYPA VP +IDHHLFFTV
Sbjct: 301 TATVHYSGTLSSSPTILTLPPPQNATSIANNFTNSLRSLNSKKYPALVPTTIDHHLFFTV 360

Query: 361 GLGINPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNY 420
           GLG+N CPTCKA NGSRVVASINNVTF MP TALL AH+F  +GVFTTDFP NPPH FNY
Sbjct: 361 GLGLNACPTCKAGNGSRVVASINNVTFIMPKTALLPAHYFNTSGVFTTDFPKNPPHVFNY 420

Query: 421 TGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFN 480
           +G    N+ T +GT++YKL YN+TV+LVLQDTG+I PENHP+HLHGFNFFEVGRG+GNFN
Sbjct: 421 SGGSVTNMATETGTRLYKLPYNATVQLVLQDTGVIAPENHPVHLHGFNFFEVGRGLGNFN 480

Query: 481 SKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVE 540
           S  DPK FNLVDPVERNTIGVPSGGW  IRFRADNPGVWFMHCHLE+HTTWGLKMAFLVE
Sbjct: 481 STKDPKNFNLVDPVERNTIGVPSGGWVVIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVE 540

Query: 541 NGKGPKQSLLPPPSDLPKC 560
           NGKGP QS+LPPP DLPKC
Sbjct: 541 NGKGPNQSILPPPKDLPKC 558

BLAST of Spg033176 vs. TAIR 10
Match: AT5G01190.1 (laccase 10 )

HSP 1 Score: 874.4 bits (2258), Expect = 4.9e-254
Identity = 403/551 (73.14%), Postives = 464/551 (84.21%), Query Frame = 0

Query: 10  LVLAFCCLLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLYAREDDTVLI 69
           LVL      PA     +R Y FNVV ++ TR+CS+K IVTVNG FPGPT+YA EDDT+L+
Sbjct: 8   LVLFALLAFPACVHGAIRKYTFNVVTKQVTRICSTKQIVTVNGKFPGPTIYANEDDTILV 67

Query: 70  KVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQRGTLFWHAH 129
            VVN+VKYN+SIHWHGIRQLRTGWADGPAYITQCPI+PG SYVYNFT+TGQRGTL+WHAH
Sbjct: 68  NVVNNVKYNVSIHWHGIRQLRTGWADGPAYITQCPIKPGHSYVYNFTVTGQRGTLWWHAH 127

Query: 130 ILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEALKSGLAPNVS 189
           +LWLRATVHGAIVILPKLG+PYPFP PH+E V++L EWWKSDTE V+NEALKSGLAPNVS
Sbjct: 128 VLWLRATVHGAIVILPKLGLPYPFPKPHREEVIILGEWWKSDTETVVNEALKSGLAPNVS 187

Query: 190 DAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGHKITVVEVDA 249
           DAH ING  G + NCPSQ  F L V+ GKTY+LR+INAALNEELFFK+AGH+ TVVEVDA
Sbjct: 188 DAHVINGHPGFVPNCPSQGNFKLAVESGKTYMLRLINAALNEELFFKIAGHRFTVVEVDA 247

Query: 250 TYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMD-APVAVDNKTATATVHYTG 309
            YVKPF TD +L+APGQTT  L++A + SG+Y++A +PF D A VAVDN+TATATVHY+G
Sbjct: 248 VYVKPFNTDTILIAPGQTTTALVSAARPSGQYLIAAAPFQDSAVVAVDNRTATATVHYSG 307

Query: 310 TLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTVGLGINPCP 369
           TL+ + T +T+PPPQNAT +ANTF NSLRSLNSK YPA VP+++DH L FTVGLGIN C 
Sbjct: 308 TLSATPTKTTSPPPQNATSVANTFVNSLRSLNSKTYPANVPITVDHDLLFTVGLGINRCH 367

Query: 370 TCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNYTGPGPKNL 429
           +CKA N SRVVA+INN+TF MP TALLQAH+F + G++TTDFP  P   F++TG  P NL
Sbjct: 368 SCKAGNFSRVVAAINNITFKMPKTALLQAHYFNLTGIYTTDFPAKPRRVFDFTGKPPSNL 427

Query: 430 QTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFNSKTDPKKF 489
            T   TK+YKL YNSTV++VLQDTG + PENHPIHLHGFNFF VG G GN+NSK D  KF
Sbjct: 428 ATMKATKLYKLPYNSTVQVVLQDTGNVAPENHPIHLHGFNFFVVGLGTGNYNSKKDSNKF 487

Query: 490 NLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVENGKGPKQS 549
           NLVDPVERNT+GVPSGGW AIRFRADNPGVWFMHCHLE+HTTWGLKMAFLVENGKGP QS
Sbjct: 488 NLVDPVERNTVGVPSGGWAAIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVENGKGPNQS 547

Query: 550 LLPPPSDLPKC 560
           + PPPSDLPKC
Sbjct: 548 IRPPPSDLPKC 558

BLAST of Spg033176 vs. TAIR 10
Match: AT5G58910.1 (laccase 16 )

HSP 1 Score: 788.5 bits (2035), Expect = 3.5e-228
Identity = 378/532 (71.05%), Postives = 437/532 (82.14%), Query Frame = 0

Query: 35  LRKATRLCSSKPIVTVNGLFPGPTLYAREDDTVLIKVVNHVKYNLSIHWHGIRQLRTGWA 94
           +   T+LCSSKPIVTVNG FPGPT+ ARE DT+LIKVVNHVKYN+SIHW       TGWA
Sbjct: 1   MTNTTKLCSSKPIVTVNGQFPGPTIVAREGDTILIKVVNHVKYNVSIHW-------TGWA 60

Query: 95  DGPAYITQCPIQPGQSYVYNFTITGQRGTLFWHAHILWLRATVHGAIVILPKLGVPYPFP 154
           DGPAYITQCPIQPGQ+Y++NFT+TGQRGTL+WHAHILWLRATVHGAIVILPKLGVPYPFP
Sbjct: 61  DGPAYITQCPIQPGQNYLHNFTLTGQRGTLWWHAHILWLRATVHGAIVILPKLGVPYPFP 120

Query: 155 TPHKETVLVLAEWWKSDTEAVINEALKSGLAPNVSDAHTINGLSGSISNCPSQRGFTLPV 214
            P+KE  +VL+EWWKSD E +INEA + G AP+ SDAHTING SGSISNCPSQ  + LPV
Sbjct: 121 KPYKEKTIVLSEWWKSDVEELINEASRIGTAPSASDAHTINGHSGSISNCPSQSSYGLPV 180

Query: 215 QKGKTYLLRIINAALNEELFFKVAGHKITVVEVDATYVKPFKTDVVLVAPGQTTNVLLTA 274
           + GKTY+LRIINAALNEELFFK+AGH +TVVEVDA Y KP+KTD V +APGQTTNVLLTA
Sbjct: 181 RAGKTYMLRIINAALNEELFFKIAGHVLTVVEVDAVYTKPYKTDTVFIAPGQTTNVLLTA 240

Query: 275 DKNSG-KYMVAVSPFMDAPVAVDNKTATATVHY---TGTLATS-LTTSTNPPPQNATQIA 334
           + N+G  YMVA + F DA +  DN TATAT+HY   T T++TS  T   + PPQNAT +A
Sbjct: 241 NANAGSNYMVAATTFTDAHIPYDNVTATATLHYIGHTSTVSTSKKTVLASLPPQNATWVA 300

Query: 335 NTFTNSLRSLNSKKYPAKVPLSIDHHLFFTVGLGINPCPTCKAANGSRVVASINNVTFAM 394
             FT SLRSLNS +YPA+VP +++H LFFTVGLG NPC +C   NG R+VA INNVTF M
Sbjct: 301 TKFTRSLRSLNSLEYPARVPTTVEHSLFFTVGLGANPCQSCN--NGVRLVAGINNVTFTM 360

Query: 395 PTTALLQAHFFKINGVFTTDFPGNPPHKFNYTGPGP--KNLQTTSGTKVYKLQYNSTVEL 454
           P TALLQAHFF I+GVFT DFP  P + ++YT P     N  T  GTK+Y+L YN+TV++
Sbjct: 361 PKTALLQAHFFNISGVFTDDFPAKPSNPYDYTAPVKLGVNAATMKGTKLYRLPYNATVQI 420

Query: 455 VLQDTGIITPENHPIHLHGFNFFEVGRGIGNFNSKTDPKKFNLVDPVERNTIGVPSGGWT 514
           VLQ+T +I  +NHP HLHGFNFFEVGRG+GNFN + DPK FNLVDPVERNT+GVP+GGWT
Sbjct: 421 VLQNTAMILSDNHPFHLHGFNFFEVGRGLGNFNPEKDPKAFNLVDPVERNTVGVPAGGWT 480

Query: 515 AIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVENGKGPKQSLLPPPSDLPKC 560
           AIRF ADNPGVWFMHCHLE+HTTWGLKMAF+V+NG GP QSLLPPP+DLPKC
Sbjct: 481 AIRFIADNPGVWFMHCHLELHTTWGLKMAFVVDNGHGPDQSLLPPPADLPKC 523

BLAST of Spg033176 vs. TAIR 10
Match: AT5G03260.1 (laccase 11 )

HSP 1 Score: 729.2 bits (1881), Expect = 2.6e-210
Identity = 338/556 (60.79%), Postives = 423/556 (76.08%), Query Frame = 0

Query: 9   FLVLAFCCLLPAL----AESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLYARED 68
           FL L FC LL  L     ++ V+ Y+F+V ++  +R+C++KPIVTVNG+FPGPT+YARE 
Sbjct: 5   FLFL-FCYLLAFLGYSPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYAREG 64

Query: 69  DTVLIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQRGTL 128
           D V+I V NHV+YN+SIHWHG++Q R GWADGPAYITQCPIQ GQSY+Y+F +TGQRGTL
Sbjct: 65  DRVIINVTNHVQYNMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVTGQRGTL 124

Query: 129 FWHAHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEALKSGL 188
           +WHAHILWLRATV+GAIVILP  G PYPFP P++E+ ++L EWW  D E  +N+A + G 
Sbjct: 125 WWHAHILWLRATVYGAIVILPAPGKPYPFPQPYQESNIILGEWWNKDVETAVNQANQLGA 184

Query: 189 APNVSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGHKITV 248
            P +SDAHTING  G +  C  +  F +  + GKTYLLRIINAALN+ELFF +AGH +TV
Sbjct: 185 PPPMSDAHTINGKPGPLFPCSEKHTFVIEAEAGKTYLLRIINAALNDELFFGIAGHNMTV 244

Query: 249 VEVDATYVKPFKTDVVLVAPGQTTNVLLTADKNSGKYMVAVSPFMDAPVAVDNKTATATV 308
           VE+DA Y KPF T  +L+ PGQTTNVL+  D++  +Y +A SPFMDAPV+VDNKT TA +
Sbjct: 245 VEIDAVYTKPFTTKAILLGPGQTTNVLVKTDRSPNRYFMAASPFMDAPVSVDNKTVTAIL 304

Query: 309 HYTGTLATSLTTSTNPPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDHHLFFTVGLGI 368
            Y G   T L      P  N T  A  +   L+SLN+  +PA VPL +D  LF+T+GLGI
Sbjct: 305 QYKGVPNTVLPILPKLPLPNDTSFALDYNGKLKSLNTPNFPALVPLKVDRRLFYTIGLGI 364

Query: 369 NPCPTCKAANGSRVVASINNVTFAMPTTALLQAHFFKINGVFTTDFPGNPPHKFNYTG-P 428
           N CPTC   NG+ + ASINN+TF MP TALL+AH+  I+GVF TDFP  PP  FNYTG P
Sbjct: 365 NACPTC--VNGTNLAASINNITFIMPKTALLKAHYSNISGVFRTDFPDRPPKAFNYTGVP 424

Query: 429 GPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGFNFFEVGRGIGNFNSKT 488
              NL T++GT++ ++++N+T+ELVLQDT ++T E+HP HLHG+NFF VG G+GNF+ K 
Sbjct: 425 LTANLGTSTGTRLSRVKFNTTIELVLQDTNLLTVESHPFHLHGYNFFVVGTGVGNFDPKK 484

Query: 489 DPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEIHTTWGLKMAFLVENGK 548
           DP KFNLVDP ERNT+GVP+GGW AIRFRADNPGVWFMHCHLE+HT WGLKMAF+VENG+
Sbjct: 485 DPAKFNLVDPPERNTVGVPTGGWAAIRFRADNPGVWFMHCHLEVHTMWGLKMAFVVENGE 544

Query: 549 GPKQSLLPPPSDLPKC 560
            P+ S+LPPP D P C
Sbjct: 545 TPELSVLPPPKDYPSC 557

BLAST of Spg033176 vs. TAIR 10
Match: AT5G60020.1 (laccase 17 )

HSP 1 Score: 664.5 bits (1713), Expect = 7.7e-191
Identity = 325/572 (56.82%), Postives = 406/572 (70.98%), Query Frame = 0

Query: 10  LVLAFCC--LLPALAESRVRHYKFNVVLRKATRLCSSKPIVTVNGLFPGPTLYAREDDTV 69
           L+  F C  LLP  A    RHY   + ++  TRLC +K +V+VNG FPGP L ARE D V
Sbjct: 6   LLAVFSCVLLLPQPAFGITRHYTLEIKMQNVTRLCHTKSLVSVNGQFPGPKLIAREGDQV 65

Query: 70  LIKVVNHVKYNLSIHWHGIRQLRTGWADGPAYITQCPIQPGQSYVYNFTITGQRGTLFWH 129
           LIKVVN V  N+S+HWHGIRQLR+GWADGPAYITQCPIQ GQSYVYN+TI GQRGTL++H
Sbjct: 66  LIKVVNQVPNNISLHWHGIRQLRSGWADGPAYITQCPIQTGQSYVYNYTIVGQRGTLWYH 125

Query: 130 AHILWLRATVHGAIVILPKLGVPYPFPTPHKETVLVLAEWWKSDTEAVINEALKSGLAPN 189
           AHI WLR+TV+G ++ILPK GVPYPF  PHKE  ++  EW+ +DTEA+I +A ++G  PN
Sbjct: 126 AHISWLRSTVYGPLIILPKRGVPYPFAKPHKEVPMIFGEWFNADTEAIIRQATQTGGGPN 185

Query: 190 VSDAHTINGLSGSISNCPSQRGFTLPVQKGKTYLLRIINAALNEELFFKVAGHKITVVEV 249
           VSDA+TINGL G + NC ++  F L V+ GKTYLLR+INAALN+ELFF +A H +TVVE 
Sbjct: 186 VSDAYTINGLPGPLYNCSAKDTFRLRVKPGKTYLLRLINAALNDELFFSIANHTVTVVEA 245

Query: 250 DATYVKPFKTDVVLVAPGQTTNVLLTADKN--SGKYMVAVSPFMDAPVAVDNKTATATVH 309
           DA YVKPF+T+ +L+APGQTTNVLL    +  S  + +   P++      DN T    + 
Sbjct: 246 DAIYVKPFETETILIAPGQTTNVLLKTKSSYPSASFFMTARPYVTGQGTFDNSTVAGILE 305

Query: 310 YTGTLAT----SLTTSTN-------PPPQNATQIANTFTNSLRSLNSKKYPAKVPLSIDH 369
           Y     T    S T+  N        P  N T  A  F+N LRSLNSK +PA VPL++D 
Sbjct: 306 YEPPKQTKGAHSRTSIKNLQLFKPILPALNDTNFATKFSNKLRSLNSKNFPANVPLNVDR 365

Query: 370 HLFFTVGLGINPC-----PTCKA-ANGSRVVASINNVTFAMPTTALLQAHFF-KINGVFT 429
             FFTVGLG NPC      TC+   N +   ASI+N++F MPT ALLQ+H+  + +GV++
Sbjct: 366 KFFFTVGLGTNPCNHKNNQTCQGPTNTTMFAASISNISFTMPTKALLQSHYSGQSHGVYS 425

Query: 430 TDFPGNPPHKFNYTGPGPKNLQTTSGTKVYKLQYNSTVELVLQDTGIITPENHPIHLHGF 489
             FP +P   FNYTG  P N   ++GT +  L YN++VELV+QDT I+  E+HP+HLHGF
Sbjct: 426 PKFPWSPIVPFNYTGTPPNNTMVSNGTNLMVLPYNTSVELVMQDTSILGAESHPLHLHGF 485

Query: 490 NFFEVGRGIGNFNSKTDPKKFNLVDPVERNTIGVPSGGWTAIRFRADNPGVWFMHCHLEI 549
           NFF VG+G GNF+   DP+ FNLVDP+ERNT+GVPSGGW AIRF ADNPGVWFMHCHLE+
Sbjct: 486 NFFVVGQGFGNFDPNKDPRNFNLVDPIERNTVGVPSGGWAAIRFLADNPGVWFMHCHLEV 545

Query: 550 HTTWGLKMAFLVENGKGPKQSLLPPPSDLPKC 560
           HT+WGL+MA+LV +G  P Q LLPPP+DLPKC
Sbjct: 546 HTSWGLRMAWLVLDGDKPDQKLLPPPADLPKC 577

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904173.10.0e+0094.63laccase-4-like [Benincasa hispida][more]
XP_023539306.10.0e+0094.28laccase-4-like [Cucurbita pepo subsp. pepo][more]
XP_022937901.10.0e+0094.28laccase-4-like [Cucurbita moschata][more]
XP_022971405.10.0e+0094.28laccase-4-like [Cucurbita maxima][more]
XP_022145678.10.0e+0093.93laccase-4-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
O804341.1e-26977.82Laccase-4 OS=Arabidopsis thaliana OX=3702 GN=IRX12 PE=2 SV=2[more]
Q6ID186.9e-25373.14Laccase-10 OS=Arabidopsis thaliana OX=3702 GN=LAC10 PE=2 SV=1[more]
Q0IQU19.7e-24769.96Laccase-22 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC22 PE=2 SV=2[more]
Q1PDH61.3e-23871.61Laccase-16 OS=Arabidopsis thaliana OX=3702 GN=LAC16 PE=2 SV=2[more]
Q8VZA13.6e-20960.79Laccase-11 OS=Arabidopsis thaliana OX=3702 GN=LAC11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1I5M90.0e+0094.28Laccase OS=Cucurbita maxima OX=3661 GN=LOC111470137 PE=3 SV=1[more]
A0A6J1FBN40.0e+0094.28Laccase OS=Cucurbita moschata OX=3662 GN=LOC111444151 PE=3 SV=1[more]
A0A6J1CWL90.0e+0093.93Laccase OS=Momordica charantia OX=3673 GN=LOC111015070 PE=3 SV=1[more]
A0A6J1J7E90.0e+0092.84Laccase OS=Cucurbita maxima OX=3661 GN=LOC111481928 PE=3 SV=1[more]
A0A5D3DMZ00.0e+0093.56Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001020 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT2G38080.17.5e-27177.82Laccase/Diphenol oxidase family protein [more]
AT5G01190.14.9e-25473.14laccase 10 [more]
AT5G58910.13.5e-22871.05laccase 16 [more]
AT5G03260.12.6e-21060.79laccase 11 [more]
AT5G60020.17.7e-19156.82laccase 17 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008972CupredoxinGENE3D2.60.40.420coord: 368..559
e-value: 4.5E-61
score: 208.2
IPR008972CupredoxinGENE3D2.60.40.420coord: 19..145
e-value: 2.4E-34
score: 120.4
IPR008972CupredoxinGENE3D2.60.40.420coord: 157..342
e-value: 1.7E-41
score: 144.5
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 18..169
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 349..559
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 153..322
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 37..146
e-value: 1.0E-39
score: 135.2
IPR017761LaccaseTIGRFAMTIGR03389TIGR03389coord: 25..559
e-value: 5.0E-281
score: 931.1
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 159..308
e-value: 8.6E-42
score: 143.0
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 413..542
e-value: 2.3E-40
score: 137.5
NoneNo IPR availablePANTHERPTHR11709:SF368LACCASEcoord: 5..559
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 5..559
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 522..533
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 517..537
IPR034285Laccase, second cupredoxin domainCDDcd13875CuRO_2_LCC_plantcoord: 161..306
e-value: 1.8913E-80
score: 246.741
IPR034289Laccase, third cupredoxin domainCDDcd13897CuRO_3_LCC_plantcoord: 405..542
e-value: 1.16151E-85
score: 259.884
IPR034288Laccase, first cupredoxin domainCDDcd13849CuRO_1_LCC_plantcoord: 29..145
e-value: 2.59353E-73
score: 227.142

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg033176.1Spg033176.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046274 lignin catabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0052716 hydroquinone:oxygen oxidoreductase activity
molecular_function GO:0016491 oxidoreductase activity