Spg033174 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg033174
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	scaffold5: 4775585 .. 4776835 (+)
RNA-Seq Expression	Spg033174
Synteny	Spg033174

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAAACACTAAGCTTATCTCTGCAATAACAGGGCCGAGGAGGTAACCCACTCGAATATTTCACTAACTTTATCCCTACTCAGCATTAGCTAAGCATTGTTCTTAAATTTAATCCTCAAGTTTCATTTTTTTTTCTTTGCTTTGTCGTTGATCAGGCTAGAAGGAAAGGTAGCAATAATAACCGGGGGAGCCAGCGGAATAGGCGAATGCACAGCAAAATTGTTCGTCCACCACGGCGCCAAAGTAGTAATAGCCGACATCCAAGACGACCTCGGCCAAACACTCTGCACCACCACACTCGGCCCAACCAACTCCCTCTACATCCACTGCGACGTCACCAACGAATCCCAAATCCAAGCCGCCATTGAAACCACCCTCAAAACCTTTGGCAAGCTTGACATCATGTTCAACAACGCCGGCATAACCGGTCCCAACAATCCCCGAATCATCGACAACCTCAAAGACGACTTCGAACGAGTCCTCTCCGTCAACGTCACAGGTGTGTTCCTCGGTATCAAACACGCATCGCAGGCCATGATCCCAGCCAAAACCGGCTCCATAATATCGACCGCGAGCGTGGTGTCGTATGTCGGCGGTGCGGCCTCGCATGCTTATACGTGCTCGAAGCATGCAGTGGTGGGGCTGACGAAGAATGCAGCCGTGGAGTTGGGGCAGTTTGGGATAAGGGTGAATTGCTTGTCGCCGTATGCATTGGAGACGCCTTTGGCTACCAAGTTTTTTGGGTTGGAGGGTGAGGATTTTGAGAAGTCTATGAACGCTGCGGCCAATTTGAAGGGGGTTACGCTTAAGGCTGAGGATGTGGCTAATGCAGCTTTGTTTTTCGCAAGTGATGAAAGTAGGTATGTGAGTGGCCATAATTTGTTGATCGATGGCGGGTTTGGTATTGTTAATTCATCAATCAAATTATTTCAGTACCCTGAAATGTAGATGGACTGGCTGATCACGAGAAATTATTCAAGGAGGTTGGTACGATGTGTGTTTGGAAGTTTTATACCACTGTTATGGGAGAGAGATATTGAAATAAAATTATGGTTTCATCATACATCCAACCGCTTAAGTGTGGTAATGACAATAACGCGACGTCCATATTGAATAATTTTCTAGTAATCTTTGTTTACTAGCTTTGTGGTTCCTTTTTTAAAATAAAGACTAGTTCTTTTTCTTTTTCATGTAATTTATTTTTGTAATAAGGTTTTATTCAAGAAATAATTACGGTTTCATTGTACAT

mRNA sequence

ATGACAAACACTAAGCTTATCTCTGCAATAACAGGGCCGAGGAGGCTAGAAGGAAAGGTAGCAATAATAACCGGGGGAGCCAGCGGAATAGGCGAATGCACAGCAAAATTGTTCGTCCACCACGGCGCCAAAGTAGTAATAGCCGACATCCAAGACGACCTCGGCCAAACACTCTGCACCACCACACTCGGCCCAACCAACTCCCTCTACATCCACTGCGACGTCACCAACGAATCCCAAATCCAAGCCGCCATTGAAACCACCCTCAAAACCTTTGGCAAGCTTGACATCATGTTCAACAACGCCGGCATAACCGGTCCCAACAATCCCCGAATCATCGACAACCTCAAAGACGACTTCGAACGAGTCCTCTCCGTCAACGTCACAGGTGTGTTCCTCGGTATCAAACACGCATCGCAGGCCATGATCCCAGCCAAAACCGGCTCCATAATATCGACCGCGAGCGTGGTGTCGTATGTCGGCGGTGCGGCCTCGCATGCTTATACGTGCTCGAAGCATGCAGTGGTGGGGCTGACGAAGAATGCAGCCGTGGAGTTGGGGCAGTTTGGGATAAGGGTGAATTGCTTGTCGCCGTATGCATTGGAGACGCCTTTGGCTACCAAGTTTTTTGGGTTGGAGGGTGAGGATTTTGAGAAGTCTATGAACGCTGCGGCCAATTTGAAGGGGGTTACGCTTAAGGCTGAGGATGTGGCTAATGCAGCTTTGTTTTTCGCAAGTGATGAAAGTAGGTATGTGAGTGGCCATAATTTGTTGATCGATGGCGGGTTTGGTATTGTTAATTCATCAATCAAATTATTTCAGTACCCTGAAATGTAG

Coding sequence (CDS)

ATGACAAACACTAAGCTTATCTCTGCAATAACAGGGCCGAGGAGGCTAGAAGGAAAGGTAGCAATAATAACCGGGGGAGCCAGCGGAATAGGCGAATGCACAGCAAAATTGTTCGTCCACCACGGCGCCAAAGTAGTAATAGCCGACATCCAAGACGACCTCGGCCAAACACTCTGCACCACCACACTCGGCCCAACCAACTCCCTCTACATCCACTGCGACGTCACCAACGAATCCCAAATCCAAGCCGCCATTGAAACCACCCTCAAAACCTTTGGCAAGCTTGACATCATGTTCAACAACGCCGGCATAACCGGTCCCAACAATCCCCGAATCATCGACAACCTCAAAGACGACTTCGAACGAGTCCTCTCCGTCAACGTCACAGGTGTGTTCCTCGGTATCAAACACGCATCGCAGGCCATGATCCCAGCCAAAACCGGCTCCATAATATCGACCGCGAGCGTGGTGTCGTATGTCGGCGGTGCGGCCTCGCATGCTTATACGTGCTCGAAGCATGCAGTGGTGGGGCTGACGAAGAATGCAGCCGTGGAGTTGGGGCAGTTTGGGATAAGGGTGAATTGCTTGTCGCCGTATGCATTGGAGACGCCTTTGGCTACCAAGTTTTTTGGGTTGGAGGGTGAGGATTTTGAGAAGTCTATGAACGCTGCGGCCAATTTGAAGGGGGTTACGCTTAAGGCTGAGGATGTGGCTAATGCAGCTTTGTTTTTCGCAAGTGATGAAAGTAGGTATGTGAGTGGCCATAATTTGTTGATCGATGGCGGGTTTGGTATTGTTAATTCATCAATCAAATTATTTCAGTACCCTGAAATGTAG

Protein sequence

MTNTKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTLGPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFERVLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAALFFASDESRYVSGHNLLIDGGFGIVNSSIKLFQYPEM

Homology

[Close]

BLAST of Spg033174 vs. NCBI nr
Match: XP_038903548.1 (secoisolariciresinol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 472.2 bits (1214), Expect = 3.0e-129
Identity = 233/277 (84.12%), Postives = 255/277 (92.06%), Query Frame = 0


Query: 1   MTNTKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCT 60
           MT++++ISA TGPRRLEGKVA+ITGGASGIGECTAKLFVHHGAKVVIAD+QDDLG  LC 
Sbjct: 1   MTSSEVISAATGPRRLEGKVALITGGASGIGECTAKLFVHHGAKVVIADVQDDLGHALCN 60

Query: 61  TTLGPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDF 120
           T LGPTNSLY+HCDVT+ESQ+QAA+   +KTFGKLDIM NNAGI  P+ PRIIDN K DF
Sbjct: 61  TILGPTNSLYVHCDVTDESQVQAAVVAAVKTFGKLDIMMNNAGIADPSKPRIIDNEKHDF 120

Query: 121 ERVLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTK 180
           +RVLSVNVTGVFLGIKHA+QAMIPAKTGSI+STASV SYVGG+ASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLSVNVTGVFLGIKHAAQAMIPAKTGSILSTASVASYVGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPYAL TPLATKF  LEGE+FE +MN+ ANLKGVTLKA+DVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYALVTPLATKFTRLEGEEFEMAMNSMANLKGVTLKAQDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFGIVNSSIKLFQYPE 278
           ALF ASDESRYVSGHNLLIDGGFGIVN +IK+FQYPE
Sbjct: 241 ALFLASDESRYVSGHNLLIDGGFGIVNPNIKIFQYPE 277

[Close]

BLAST of Spg033174 vs. NCBI nr
Match: XP_023527970.1 (secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 461.1 bits (1185), Expect = 6.9e-126
Identity = 228/275 (82.91%), Postives = 252/275 (91.64%), Query Frame = 0


Query: 4   TKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTL 63
           ++LISA T PRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQ LC T L
Sbjct: 3   SQLISAPTAPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQALCATAL 62

Query: 64  -GPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFER 123
            GPTNSLY+HCDVT+ESQI+AA+ET +K FGKLDIM NNAGI  P+ PRIIDN K+DFER
Sbjct: 63  GGPTNSLYVHCDVTDESQIKAAVETAVKAFGKLDIMMNNAGIADPSKPRIIDNQKEDFER 122

Query: 124 VLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNA 183
           V+SVNVTGVFLGIKHA+QAMIP KTGSIISTAS+ SYVGG+ASHAYTCSKHAVVGLTKNA
Sbjct: 123 VISVNVTGVFLGIKHAAQAMIPVKTGSIISTASLASYVGGSASHAYTCSKHAVVGLTKNA 182

Query: 184 AVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAAL 243
           AVELGQFGIRVNCLSP+A+ TPLAT+F GLEGE+FE +MN+ ANLKGVTL+ EDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPFAMVTPLATRFVGLEGEEFEMAMNSMANLKGVTLRTEDVANAAL 242

Query: 244 FFASDESRYVSGHNLLIDGGFGIVNSSIKLFQYPE 278
           F ASDESRYVSGHNLLIDGGFGI N +IK+F+YP+
Sbjct: 243 FLASDESRYVSGHNLLIDGGFGITNPNIKIFEYPQ 277

[Close]

BLAST of Spg033174 vs. NCBI nr
Match: XP_022934666.1 (secoisolariciresinol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 456.4 bits (1173), Expect = 1.7e-124
Identity = 226/275 (82.18%), Postives = 249/275 (90.55%), Query Frame = 0


Query: 4   TKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTL 63
           ++L+SA T PRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQ LC T L
Sbjct: 3   SQLMSAPTAPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQALCATAL 62

Query: 64  -GPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFER 123
            GP NSLY+HCDVT ESQI+AA+E  +K FGKLDIM NNAGI  P+ PRIIDN K+DFER
Sbjct: 63  GGPINSLYVHCDVTEESQIKAAVEAAVKAFGKLDIMMNNAGIADPSKPRIIDNQKEDFER 122

Query: 124 VLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNA 183
           V+SVNVTGVFLGIKHA+QAMIP KTGSIISTAS+ SYVGG+ASHAYTCSKHAVVGLTKNA
Sbjct: 123 VISVNVTGVFLGIKHAAQAMIPVKTGSIISTASLASYVGGSASHAYTCSKHAVVGLTKNA 182

Query: 184 AVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAAL 243
           AVELGQFGIRVNCLSP+A+ TPLAT+F GLEGE+FE +MN+ ANLKGVTL+ EDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPFAMVTPLATRFVGLEGEEFETAMNSMANLKGVTLRTEDVANAAL 242

Query: 244 FFASDESRYVSGHNLLIDGGFGIVNSSIKLFQYPE 278
           F ASDESRYVSGHNLLIDGGFGI N +IK+F+YPE
Sbjct: 243 FLASDESRYVSGHNLLIDGGFGITNPNIKIFEYPE 277

[Close]

BLAST of Spg033174 vs. NCBI nr
Match: KAG6580723.1 (hypothetical protein SDJN03_20725, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 456.4 bits (1173), Expect = 1.7e-124
Identity = 225/272 (82.72%), Postives = 248/272 (91.18%), Query Frame = 0


Query: 7   ISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTL-GP 66
           ISA T PRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQ LC T L GP
Sbjct: 6   ISATTAPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQALCATALGGP 65

Query: 67  TNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFERVLS 126
           TNSLY+HCDVT+E+QI+AA+E  +K FGKLDIM NNAGI  P+ PRIIDN K+DFERV+S
Sbjct: 66  TNSLYVHCDVTDEAQIKAAVEAAVKAFGKLDIMMNNAGIADPSKPRIIDNQKEDFERVIS 125

Query: 127 VNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNAAVE 186
           VNVTGVFLGIKHA+QAMIP KTGSIISTAS+ SYVGG+ASHAYTCSKHAVVGLTKNAAVE
Sbjct: 126 VNVTGVFLGIKHAAQAMIPVKTGSIISTASLASYVGGSASHAYTCSKHAVVGLTKNAAVE 185

Query: 187 LGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAALFFA 246
           LGQFGIRVNCLSP+A+ TPLAT+F GLEGE+FE +MN+ ANLKGVTL+ EDVANAALF A
Sbjct: 186 LGQFGIRVNCLSPFAMVTPLATRFVGLEGEEFEMAMNSMANLKGVTLRTEDVANAALFLA 245

Query: 247 SDESRYVSGHNLLIDGGFGIVNSSIKLFQYPE 278
           SDESRYVSGHNLLIDGGFGI N +IK+F+YP+
Sbjct: 246 SDESRYVSGHNLLIDGGFGITNPNIKIFEYPQ 277

[Close]

BLAST of Spg033174 vs. NCBI nr
Match: XP_022983061.1 (secoisolariciresinol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 454.5 bits (1168), Expect = 6.5e-124
Identity = 224/275 (81.45%), Postives = 250/275 (90.91%), Query Frame = 0


Query: 4   TKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTL 63
           ++LISA T PRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQ LC T L
Sbjct: 3   SQLISAPTAPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQALCATAL 62

Query: 64  -GPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFER 123
            GPTN LY+HCDVT+E+QI+AA+E  ++ FGKLDIM NNAGI  P+ PRIIDN K+DFER
Sbjct: 63  GGPTNCLYVHCDVTDEAQIKAAVEAAVEAFGKLDIMMNNAGIADPSKPRIIDNHKEDFER 122

Query: 124 VLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNA 183
           VL++NVTGVFLGIKHA+QAMIP KTGSIISTAS+ SYVGG+ASHAYTCSKHAVVGLTKNA
Sbjct: 123 VLNINVTGVFLGIKHAAQAMIPVKTGSIISTASLASYVGGSASHAYTCSKHAVVGLTKNA 182

Query: 184 AVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAAL 243
           AVELGQFGIRVNCLSP+A+ TPLAT+F GLEGE+FE +MN+ ANLKGVTL+ EDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPFAMVTPLATRFVGLEGEEFETAMNSMANLKGVTLRTEDVANAAL 242

Query: 244 FFASDESRYVSGHNLLIDGGFGIVNSSIKLFQYPE 278
           F ASDESRYVSGHNLLIDGGFGI N +IK+F+YPE
Sbjct: 243 FLASDESRYVSGHNLLIDGGFGITNPNIKIFEYPE 277

[Close]

BLAST of Spg033174 vs. ExPASy Swiss-Prot
Match: Q94KL7 (Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.2e-96
Identity = 179/275 (65.09%), Postives = 221/275 (80.36%), Query Frame = 0


Query: 4   TKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTL 63
           +++++AI   RRLEGKVA+ITGGASGIGE TAKLF  HGAKV IAD+QD+LG ++    +
Sbjct: 5   SQVLTAIA--RRLEGKVALITGGASGIGETTAKLFSQHGAKVAIADVQDELGHSV-VEAI 64

Query: 64  GPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFERV 123
           G +NS YIHCDVTNE  ++ A++ T+ T+GKLDIMF+NAGI+ PN PRIIDN K DFERV
Sbjct: 65  GTSNSTYIHCDVTNEDGVKNAVDNTVSTYGKLDIMFSNAGISDPNRPRIIDNEKADFERV 124

Query: 124 LSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNAA 183
            SVNVTGVFL +KHA++ MIPA++G+IISTAS+ S +GG +SHAY  SKHAV+GLT+N A
Sbjct: 125 FSVNVTGVFLCMKHAARVMIPARSGNIISTASLSSTMGGGSSHAYCGSKHAVLGLTRNLA 184

Query: 184 VELGQFGIRVNCLSPYALETPLATKFFGLEG-EDFEKSMNAAANLKGVTLKAEDVANAAL 243
           VELGQFGIRVNCLSP+ L T L  KF G++  E+FE  +N A NLKG     EDVANAAL
Sbjct: 185 VELGQFGIRVNCLSPFGLPTALGKKFSGIKNEEEFENVINFAGNLKGPKFNVEDVANAAL 244

Query: 244 FFASDESRYVSGHNLLIDGGFGIVNSSIKLFQYPE 278
           + ASDE++YVSGHNL IDGGF + NS IK+FQYP+
Sbjct: 245 YLASDEAKYVSGHNLFIDGGFSVCNSVIKVFQYPD 276

[Close]

BLAST of Spg033174 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 5.2e-84
Identity = 160/262 (61.07%), Postives = 198/262 (75.57%), Query Frame = 0


Query: 14  RRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTLGPTNSLYIHC 73
           R+L GKVA+ITGGASGIG CTA+LFV HGA+VV+ADIQD+LG +L    LGP  S Y+HC
Sbjct: 13  RKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASL-VAELGPDASSYVHC 72

Query: 74  DVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFERVLSVNVTGVFL 133
           DVTNE  + AA++  +  FGKLD+MFNNAG++GP   R+ +  K+DFERVL+VN+ G FL
Sbjct: 73  DVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVGPFL 132

Query: 134 GIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRV 193
           G KHA++ M PA+ GSIISTAS+ S V GAASHAYT SKHA+VG T+NAA ELG+ GIRV
Sbjct: 133 GTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHGIRV 192

Query: 194 NCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGV-TLKAEDVANAALFFASDESRYV 253
           NC+SP  + TPLA    G++ E  E  M  +ANLKG   LKA+D+A AALF ASD+ RYV
Sbjct: 193 NCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDGRYV 252

Query: 254 SGHNLLIDGGFGIVNSSIKLFQ 275
           SG NL +DGG  +VNSS   F+
Sbjct: 253 SGQNLRVDGGLSVVNSSFGFFR 273

[Close]

BLAST of Spg033174 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 1.7e-71
Identity = 136/254 (53.54%), Postives = 176/254 (69.29%), Query Frame = 0


Query: 15  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTLGPTNSLYIHCD 74
           RL+ KVAIITGGA GIGE TAKLFV +GAKVVIADI DD GQ +C     P    ++HCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVISFVHCD 72

Query: 75  VTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFERVLSVNVTGVFLG 134
           VT +  ++  ++TT+   GKLDIMF N G+       I++   +DF+RV+ +NV G FL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 135 IKHASQAMIPAKTGSIISTASVVSYVGG-AASHAYTCSKHAVVGLTKNAAVELGQFGIRV 194
            KHA++ MIPAK GSI+ TAS+ S+  G   SH YT +KHAV+GLT +   ELGQ GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQHGIRV 192

Query: 195 NCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAALFFASDESRYVS 254
           NC+SPY + +PL T  FG++    E+  + AANLKG+ L+AEDVA+A  + A DES+YVS
Sbjct: 193 NCVSPYVVASPLLTDVFGVDSSRVEELAHQAANLKGILLRAEDVADAVAYLAGDESKYVS 252

Query: 255 GHNLLIDGGFGIVN 268
           G NL+IDGG+   N
Sbjct: 253 GLNLVIDGGYTRTN 266

[Close]

BLAST of Spg033174 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 5.1e-71
Identity = 135/254 (53.15%), Postives = 176/254 (69.29%), Query Frame = 0


Query: 15  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTLGPTNSLYIHCD 74
           RL+ KVAIITGGA GIGE TAKLFV +GAKVVIADI DD GQ +C     P    ++HCD
Sbjct: 13  RLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISFVHCD 72

Query: 75  VTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFERVLSVNVTGVFLG 134
           VT +  ++  ++TT+   GKLDIMF N G+       I++   +DF+RV+ +NV G FL 
Sbjct: 73  VTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYGAFLV 132

Query: 135 IKHASQAMIPAKTGSIISTASVVSYVGG-AASHAYTCSKHAVVGLTKNAAVELGQFGIRV 194
            KHA++ MIPAK GSI+ TAS+ S+  G   SH YT +KHAV+GLT +   ELG++GIRV
Sbjct: 133 AKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEYGIRV 192

Query: 195 NCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAALFFASDESRYVS 254
           NC+SPY + +PL T  FG++    E+  + AANLKG  L+AEDVA+A  + A DES+YVS
Sbjct: 193 NCVSPYIVASPLLTDVFGVDSSRVEELAHQAANLKGTLLRAEDVADAVAYLAGDESKYVS 252

Query: 255 GHNLLIDGGFGIVN 268
           G NL+IDGG+   N
Sbjct: 253 GLNLVIDGGYTRTN 266

[Close]

BLAST of Spg033174 vs. ExPASy Swiss-Prot
Match: F1SWA0 (Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 3.3e-70
Identity = 138/268 (51.49%), Postives = 184/268 (68.66%), Query Frame = 0


Query: 15  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTLGPTNSLYIHCD 74
           RLEGKVA++TGGASGIGE  A+LF+ HGAK+ I D+QD+LGQ +     G  ++ Y HCD
Sbjct: 2   RLEGKVALVTGGASGIGESIARLFIEHGAKICIVDVQDELGQQVSQRLGGDPHACYFHCD 61

Query: 75  VTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFERVLSVNVTGVFLG 134
           VT E  ++ A++ T + +G +DIM NNAGITG     I D   ++F++V  +NV GVFLG
Sbjct: 62  VTVEDDVRRAVDFTAEKYGTIDIMVNNAGITGDKVIDIRDADFNEFKKVFDINVNGVFLG 121

Query: 135 IKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRVN 194
           +KHA++ MIP   GSI+S ASV S + GA  H YT +KHAVVGLTK+ A ELG+ GIRVN
Sbjct: 122 MKHAARIMIPKMKGSIVSLASVSSVIAGAGPHGYTGAKHAVVGLTKSVAAELGRHGIRVN 181

Query: 195 CLSPYALETPLATKFF--------GLEGEDFEKSMNAAANLKGVTLKAEDVANAALFFAS 254
           C+SPYA+ T L+  +          L G  F   + + ANLKGV L   DVA A L+ A+
Sbjct: 182 CVSPYAVPTRLSMPYLPESEMQEDALRG--FLTFVRSNANLKGVDLMPNDVAEAVLYLAT 241

Query: 255 DESRYVSGHNLLIDGGFGIVNSSIKLFQ 275
           +ES+YVSG NL+IDGGF I N ++++F+
Sbjct: 242 EESKYVSGLNLVIDGGFSIANHTLQVFE 267

[Close]

BLAST of Spg033174 vs. ExPASy TrEMBL
Match: A0A6J1F2F3 (secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111441789 PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 8.2e-125
Identity = 226/275 (82.18%), Postives = 249/275 (90.55%), Query Frame = 0


Query: 4   TKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTL 63
           ++L+SA T PRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQ LC T L
Sbjct: 3   SQLMSAPTAPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQALCATAL 62

Query: 64  -GPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFER 123
            GP NSLY+HCDVT ESQI+AA+E  +K FGKLDIM NNAGI  P+ PRIIDN K+DFER
Sbjct: 63  GGPINSLYVHCDVTEESQIKAAVEAAVKAFGKLDIMMNNAGIADPSKPRIIDNQKEDFER 122

Query: 124 VLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNA 183
           V+SVNVTGVFLGIKHA+QAMIP KTGSIISTAS+ SYVGG+ASHAYTCSKHAVVGLTKNA
Sbjct: 123 VISVNVTGVFLGIKHAAQAMIPVKTGSIISTASLASYVGGSASHAYTCSKHAVVGLTKNA 182

Query: 184 AVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAAL 243
           AVELGQFGIRVNCLSP+A+ TPLAT+F GLEGE+FE +MN+ ANLKGVTL+ EDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPFAMVTPLATRFVGLEGEEFETAMNSMANLKGVTLRTEDVANAAL 242

Query: 244 FFASDESRYVSGHNLLIDGGFGIVNSSIKLFQYPE 278
           F ASDESRYVSGHNLLIDGGFGI N +IK+F+YPE
Sbjct: 243 FLASDESRYVSGHNLLIDGGFGITNPNIKIFEYPE 277

[Close]

BLAST of Spg033174 vs. ExPASy TrEMBL
Match: A0A6J1J123 (secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111481719 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 3.1e-124
Identity = 224/275 (81.45%), Postives = 250/275 (90.91%), Query Frame = 0


Query: 4   TKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTL 63
           ++LISA T PRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQ LC T L
Sbjct: 3   SQLISAPTAPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQALCATAL 62

Query: 64  -GPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFER 123
            GPTN LY+HCDVT+E+QI+AA+E  ++ FGKLDIM NNAGI  P+ PRIIDN K+DFER
Sbjct: 63  GGPTNCLYVHCDVTDEAQIKAAVEAAVEAFGKLDIMMNNAGIADPSKPRIIDNHKEDFER 122

Query: 124 VLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNA 183
           VL++NVTGVFLGIKHA+QAMIP KTGSIISTAS+ SYVGG+ASHAYTCSKHAVVGLTKNA
Sbjct: 123 VLNINVTGVFLGIKHAAQAMIPVKTGSIISTASLASYVGGSASHAYTCSKHAVVGLTKNA 182

Query: 184 AVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAAL 243
           AVELGQFGIRVNCLSP+A+ TPLAT+F GLEGE+FE +MN+ ANLKGVTL+ EDVANAAL
Sbjct: 183 AVELGQFGIRVNCLSPFAMVTPLATRFVGLEGEEFETAMNSMANLKGVTLRTEDVANAAL 242

Query: 244 FFASDESRYVSGHNLLIDGGFGIVNSSIKLFQYPE 278
           F ASDESRYVSGHNLLIDGGFGI N +IK+F+YPE
Sbjct: 243 FLASDESRYVSGHNLLIDGGFGITNPNIKIFEYPE 277

[Close]

BLAST of Spg033174 vs. ExPASy TrEMBL
Match: A0A0A0LGR0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778270 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.1e-121
Identity = 217/277 (78.34%), Postives = 246/277 (88.81%), Query Frame = 0


Query: 1   MTNTKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCT 60
           MT++++IS  TG RRLEGKVAIITGGASGIGECTAKLFVHHGAKVV+ADIQDDLG+ LC 
Sbjct: 1   MTSSEVISTTTGQRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVVADIQDDLGRALCA 60

Query: 61  TTLGPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDF 120
             LG TNSLY+HCDVT+ESQ+QAA+   ++TFGKLDIM NNAGI  P+ PRIIDN K DF
Sbjct: 61  NVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDIMMNNAGIADPSKPRIIDNDKHDF 120

Query: 121 ERVLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTK 180
           +RVLS+NVTGVFLGIKHA+QAMIP KTGSI+STASV SY+GG+ASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLSINVTGVFLGIKHAAQAMIPVKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPY L TPLAT+F  L+G +FEK  ++ ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYVLATPLATEFVDLDGAEFEKYASSRANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFGIVNSSIKLFQYPE 278
           ALF ASDESRYVSGHNL +DGGF IVN +I++FQYP+
Sbjct: 241 ALFLASDESRYVSGHNLFVDGGFSIVNPNIQIFQYPQ 277

[Close]

BLAST of Spg033174 vs. ExPASy TrEMBL
Match: A0A5A7TKJ5 (Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004260 PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 7.2e-121
Identity = 217/274 (79.20%), Postives = 244/274 (89.05%), Query Frame = 0


Query: 1   MTNTKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCT 60
           MT++++IS  TG RRLEGKVA+ITGGASGIGECTAK+FVHHGAKVVIADIQDDLG  LC 
Sbjct: 1   MTSSEVISLTTGQRRLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCA 60

Query: 61  TTLGPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDF 120
             LG TNSLY+HCDVT+ESQ+QAA+   ++TFGKLD+M NNAGI  P+ PRIIDN + DF
Sbjct: 61  NVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDF 120

Query: 121 ERVLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTK 180
           +RVL++NVTGVFLGIKHA+QAMIPAKTGSI+STASV SY+GG+ASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLNINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPYAL TPLATKF GL G +FE+ M + ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFGIVNSSIKLFQ 275
           ALF ASDESRYVSGHNLLIDGGF IVN +I +FQ
Sbjct: 241 ALFLASDESRYVSGHNLLIDGGFSIVNPNINIFQ 274

[Close]

BLAST of Spg033174 vs. ExPASy TrEMBL
Match: A0A1S3B7H5 (secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103486649 PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 7.2e-121
Identity = 217/274 (79.20%), Postives = 244/274 (89.05%), Query Frame = 0


Query: 1   MTNTKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCT 60
           MT++++IS  TG RRLEGKVA+ITGGASGIGECTAK+FVHHGAKVVIADIQDDLG  LC 
Sbjct: 1   MTSSEVISLTTGQRRLEGKVALITGGASGIGECTAKIFVHHGAKVVIADIQDDLGHALCA 60

Query: 61  TTLGPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDF 120
             LG TNSLY+HCDVT+ESQ+QAA+   ++TFGKLD+M NNAGI  P+ PRIIDN + DF
Sbjct: 61  NVLGSTNSLYVHCDVTDESQVQAAVAAAVETFGKLDVMMNNAGIVDPSKPRIIDNDRSDF 120

Query: 121 ERVLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTK 180
           +RVL++NVTGVFLGIKHA+QAMIPAKTGSI+STASV SY+GG+ASHAYTCSKHAVVGLTK
Sbjct: 121 DRVLNINVTGVFLGIKHAAQAMIPAKTGSILSTASVASYIGGSASHAYTCSKHAVVGLTK 180

Query: 181 NAAVELGQFGIRVNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANA 240
           NAAVELGQFGIRVNCLSPYAL TPLATKF GL G +FE+ M + ANLKGVTLKAEDVANA
Sbjct: 181 NAAVELGQFGIRVNCLSPYALVTPLATKFVGLNGAEFEEVMGSKANLKGVTLKAEDVANA 240

Query: 241 ALFFASDESRYVSGHNLLIDGGFGIVNSSIKLFQ 275
           ALF ASDESRYVSGHNLLIDGGF IVN +I +FQ
Sbjct: 241 ALFLASDESRYVSGHNLLIDGGFSIVNPNINIFQ 274

[Close]

BLAST of Spg033174 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 3.1e-68
Identity = 146/283 (51.59%), Postives = 180/283 (63.60%), Query Frame = 0


Query: 2   TNTKLISAITGP-RRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCT 61
           TNT+  S  + P +RL GKVA+ITGGA+GIGE   +LF  HGAKV I D+QDDLG  +C 
Sbjct: 3   TNTESSSYSSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCK 62

Query: 62  TTL---GPTNSLYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLK 121
           + L       + +IH DV  E  I  A++  +K FG LDI+ NNAG+ G   P I +   
Sbjct: 63  SLLRGESKETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSL 122

Query: 122 DDFERVLSVNVTGVFLGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVG 181
            +FE    VNV G FL +KHA++ MIP K GSI+S  SV   VGG   H+Y  SKHAV+G
Sbjct: 123 SEFEMTFDVNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLG 182

Query: 182 LTKNAAVELGQFGIRVNCLSPYALETPLATKFFGLE--GED----FEKSMNAAANLKGVT 241
           LT++ A ELGQ GIRVNC+SPYA+ T LA      E   ED    F     A ANLKGV 
Sbjct: 183 LTRSVAAELGQHGIRVNCVSPYAVATKLALAHLPEEERTEDAFVGFRNFAAANANLKGVE 242

Query: 242 LKAEDVANAALFFASDESRYVSGHNLLIDGGFGIVNSSIKLFQ 275
           L  +DVANA LF ASD+SRY+SG NL+IDGGF   N S K+F+
Sbjct: 243 LTVDDVANAVLFLASDDSRYISGDNLMIDGGFTCTNHSFKVFR 285

[Close]

BLAST of Spg033174 vs. TAIR 10
Match: AT3G51680.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 1.9e-65
Identity = 139/286 (48.60%), Postives = 185/286 (64.69%), Query Frame = 0


Query: 2   TNTKLISAITGPRRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTT 61
           TNT L      P+RLEGKVAIITGGA GIG+ T  LF  HGA VVIAD+ +  G +L  +
Sbjct: 23  TNTTLY-----PKRLEGKVAIITGGAHGIGKATVMLFARHGATVVIADVDNVAGSSLAKS 82

Query: 62  TLGPTNS---LYIHCDVTNESQIQAAIETTLKTFGKLDIMFNNAGITG--PNNPRIIDNL 121
                 S    +I CDV+ E+ ++  +  T+  +G+LDI+FNNAG+ G    +  I+D  
Sbjct: 83  LSSHKTSPMVAFISCDVSVEADVENLVNVTVARYGRLDILFNNAGVLGDQKKHKSILDFD 142

Query: 122 KDDFERVLSVNVTGVFLGIKHASQAMIP-AKTGSIISTASVVSYVGGAASHAYTCSKHAV 181
            D+F+ V+ VNV GV LG+KH ++AMI     G IISTASV   +GG   HAYT SKHA+
Sbjct: 143 ADEFDHVMRVNVRGVGLGMKHGARAMIKRGFKGCIISTASVAGVMGGMGPHAYTASKHAI 202

Query: 182 VGLTKNAAVELGQFGIRVNCLSPYALETPLAT----KFFGLEGED-----FEKSMNAAAN 241
           VGLTKNAA ELG++GIRVNC+SP+ + T +      K  G + ED      E+ + + AN
Sbjct: 203 VGLTKNAACELGKYGIRVNCISPFGVATSMLVNAWRKTSGGDVEDDDVEEMEEFVRSLAN 262

Query: 242 LKGVTLKAEDVANAALFFASDESRYVSGHNLLIDGGFGIVNSSIKL 273
           LKG TL+A D+A AAL+ ASDES+YV+GHNL++DGG     + + L
Sbjct: 263 LKGETLRANDIAEAALYLASDESKYVNGHNLVVDGGVTTARNCVGL 303

[Close]

BLAST of Spg033174 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 242.7 bits (618), Expect = 3.6e-64
Identity = 121/254 (47.64%), Postives = 179/254 (70.47%), Query Frame = 0


Query: 14  RRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTLGPTNSLYIHC 73
           +RL+GK+ IITGGASGIG  + +LF  HGA+VVI D+QD+LGQ +   ++G   + Y HC
Sbjct: 4   KRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNV-AVSIGEDKASYYHC 63

Query: 74  DVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFERVLSVNVTGVFL 133
           DVTNE++++ A++ T++ +GKLD++F+NAG+  P    I+D   ++ +R +++N+ G   
Sbjct: 64  DVTNETEVENAVKFTVEKYGKLDVLFSNAGVIEP-FVSILDLNLNELDRTIAINLRGTAA 123

Query: 134 GIKHASQAMI-PAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIR 193
            IKHA++AM+     GSI+ T SV + + G A H YT SKH ++GL K+A+  LG++GIR
Sbjct: 124 FIKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIR 183

Query: 194 VNCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAALFFASDESRYV 253
           VN ++P+ + TPL    F +E    E++ +A+ANLKG+ LKA  VA AALF ASDES YV
Sbjct: 184 VNGVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESAYV 243

Query: 254 SGHNLLIDGGFGIV 267
           SG NL +DGG+ +V
Sbjct: 244 SGQNLAVDGGYSVV 255

[Close]

BLAST of Spg033174 vs. TAIR 10
Match: AT3G26770.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 230.3 bits (586), Expect = 1.8e-60
Identity = 119/255 (46.67%), Postives = 169/255 (66.27%), Query Frame = 0


Query: 14  RRLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTLGPTNSLYIHC 73
           ++LEGKVA+ITGGASG+G+ TA  F+ HGA+VVIAD+  + G T     LG + + ++ C
Sbjct: 39  KKLEGKVALITGGASGLGKATASEFLRHGARVVIADLDAETG-TKTAKELG-SEAEFVRC 98

Query: 74  DVTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNL-KDDFERVLSVNVTGVF 133
           DVT E+ I  A+E T++ +GKLD+M+NNAGI GP  P  I  L   +FERV+ +NV GV 
Sbjct: 99  DVTVEADIAGAVEMTVERYGKLDVMYNNAGIVGPMTPASISQLDMTEFERVMRINVFGVV 158

Query: 134 LGIKHASQAMIPAKTGSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIR 193
            GIKHA++ MIPA++G I+ T+SV    GG A H+YT SK    G+ K+AA EL + G+R
Sbjct: 159 SGIKHAAKFMIPARSGCILCTSSVAGVTGGLAPHSYTISKFTTPGIVKSAASELCEHGVR 218

Query: 194 VNCLSPYALETPLATKFF-----GLEGEDFEKSMNAAANLKGVTLKAEDVANAALFFASD 253
           +NC+SP  + TPL   +       +  E   +++     LKG   +  DVA AAL+ AS+
Sbjct: 219 INCISPGTVATPLTLSYLQKVFPKVSEEKLRETVKGMGELKGAECEEADVAKAALYLASN 278

Query: 254 ESRYVSGHNLLIDGG 263
           + +YV+GHNL++DGG
Sbjct: 279 DGKYVTGHNLVVDGG 291

[Close]

BLAST of Spg033174 vs. TAIR 10
Match: AT2G47120.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 227.6 bits (579), Expect = 1.2e-59
Identity = 121/256 (47.27%), Postives = 173/256 (67.58%), Query Frame = 0


Query: 15  RLEGKVAIITGGASGIGECTAKLFVHHGAKVVIADIQDDLGQTLCTTTLGPTNSLYIHCD 74
           RLEGK+ IITGGASGIG   A+LF  HGAKVVI D+Q++LGQ +    +G   + +  CD
Sbjct: 5   RLEGKIVIITGGASGIGADAARLFTDHGAKVVIVDVQEELGQNV-AVLIGKDKASFYRCD 64

Query: 75  VTNESQIQAAIETTLKTFGKLDIMFNNAGITGPNNPRIIDNLKDDFERVLSVNVTGVFLG 134
           VTNE++++ A++ T++  GKLD++F+NAG+  P     +D   + F+R+++VNV G    
Sbjct: 65  VTNETEVEDAVKFTVEKHGKLDVLFSNAGVLEPLE-SFLDFDLERFDRIMAVNVRGAAAF 124

Query: 135 IKHASQAMIPAKT-GSIISTASVVSYVGGAASHAYTCSKHAVVGLTKNAAVELGQFGIRV 194
           IKHA++AM+   T GSI+ T SV + +GG   H YT SKH +VGL ++A  +LG++GIRV
Sbjct: 125 IKHAARAMVEKGTRGSIVCTTSVSAEIGG-GHHGYTASKHGLVGLIRSACGDLGKYGIRV 184

Query: 195 NCLSPYALETPLATKFFGLEGEDFEKSMNAAANLKGVTLKAEDVANAALFFASDESRYVS 254
           N ++PYA+ TP+ T    + G+  E   +A   LKG+ LKA  VA  ALF ASD+S Y+S
Sbjct: 185 NGVAPYAVATPM-TSHDEVTGKQLEDYFDAKGILKGMVLKASHVAQVALFLASDDSAYIS 244

Query: 255 GHNLLIDGGFGIVNSS 270
           G NL +DGG+ +V  S
Sbjct: 245 GQNLAVDGGYTVVKPS 256

The following BLAST results are available for this feature:

BLAST of Spg033174 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903548.1	3.0e-129	84.12	secoisolariciresinol dehydrogenase-like [Benincasa hispida]	[more]
XP_023527970.1	6.9e-126	82.91	secoisolariciresinol dehydrogenase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022934666.1	1.7e-124	82.18	secoisolariciresinol dehydrogenase-like [Cucurbita moschata]	[more]
KAG6580723.1	1.7e-124	82.72	hypothetical protein SDJN03_20725, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022983061.1	6.5e-124	81.45	secoisolariciresinol dehydrogenase-like [Cucurbita maxima]	[more]

BLAST of Spg033174 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KL7	1.2e-96	65.09	Secoisolariciresinol dehydrogenase (Fragment) OS=Forsythia intermedia OX=55183 P...	[more]
Q7FAE1	5.2e-84	61.07	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
A3F5F0	1.7e-71	53.54	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
Q94KL8	5.1e-71	53.15	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]
F1SWA0	3.3e-70	51.49	Zerumbone synthase OS=Zingiber zerumbet OX=311405 GN=ZSD1 PE=1 SV=1	[more]

BLAST of Spg033174 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F2F3	8.2e-125	82.18	secoisolariciresinol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1J123	3.1e-124	81.45	secoisolariciresinol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111481...	[more]
A0A0A0LGR0	1.1e-121	78.34	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G778270 PE=4 SV=1	[more]
A0A5A7TKJ5	7.2e-121	79.20	Secoisolariciresinol dehydrogenase-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3B7H5	7.2e-121	79.20	secoisolariciresinol dehydrogenase-like OS=Cucumis melo OX=3656 GN=LOC103486649 ...	[more]

BLAST of Spg033174 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52340.1	3.1e-68	51.59	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G51680.1	1.9e-65	48.60	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47140.1	3.6e-64	47.64	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26770.1	1.8e-60	46.67	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47120.1	1.2e-59	47.27	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 142..158 score: 32.25 coord: 227..247 score: 31.21 coord: 168..187 score: 42.87 coord: 93..104 score: 58.12 coord: 20..37 score: 48.2 coord: 189..206 score: 37.37
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 93..104 score: 51.1 coord: 148..156 score: 46.12 coord: 168..187 score: 37.39
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 25..263 e-value: 6.1E-66 score: 222.3
None	No IPR available	GENE3D	3.40.50.720		coord: 9..273 e-value: 4.2E-81 score: 273.9
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 22..214 e-value: 9.8E-19 score: 65.4
None	No IPR available	PIRSR	PIRSR000094-1	PIRSR000094-1	coord: 16..268 e-value: 1.7E-20 score: 71.1
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 16..268 e-value: 1.7E-20 score: 71.1
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 13..275
None	No IPR available	PANTHER	PTHR43180:SF50	SECOISOLARICIRESINOL DEHYDROGENASE-LIKE	coord: 13..275
None	No IPR available	CDD	cd05326	secoisolariciresinol-DH_like_SDR_c	coord: 15..263 e-value: 1.39483E-127 score: 361.003
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 155..183
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 17..265

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg033174.1	Spg033174.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity