Spg033150 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg033150 vs. NCBI nr
Match: XP_022934689.1 (uncharacterized protein LOC111441803 [Cucurbita moschata] >XP_022934690.1 uncharacterized protein LOC111441803 [Cucurbita moschata] >XP_022934691.1 uncharacterized protein LOC111441803 [Cucurbita moschata] >XP_022934692.1 uncharacterized protein LOC111441803 [Cucurbita moschata] >KAG6580439.1 hypothetical protein SDJN03_20441, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1741.1 bits (4508), Expect = 0.0e+00
Identity = 880/961 (91.57%), Postives = 914/961 (95.11%), Query Frame = 0

Query: 1   MAYISNCSTCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHSRQLQSPEIRIPRTAAKL 60
           MA+I+NCSTCNV    +K RAS+F  TLSSSFLRL+ D  +H+R+LQSP+IR+ R AAK+
Sbjct: 1   MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60

Query: 61  VAKDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
           V KDGVAEMA TE+EREK S KC ALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61  VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121 SCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIH 180
           SCYYID ELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLST VDIVFPVIH
Sbjct: 121 SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181 GRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVS 240
           GRFGEDGGIQELLE HNIPFVGTGSTEC HAFDKYNA+LELDRLGFITV NFLVQAGSVS
Sbjct: 181 GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240

Query: 241 ESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLV 300
           ES+LSKWF RNQLD SSGKVVVKPVRAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241 ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301 EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ 360
           EVFLE GSEFTAIVLDVGSGSVCHPVVLLPTEVEL+ H WADAGEKDAIFNYRRKYLPTQ
Sbjct: 301 EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360

Query: 361 QVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTE 420
           QVAYHTPPRFPI+VIESIREGASLLFKGLGL DFARIDGWFLPNSSH+ S STGKFGRT 
Sbjct: 361 QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNF 480
           SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 
Sbjct: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481 PRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL 540
           PRRSISSQR+Q YSS ES +KVFVI GGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCLL
Sbjct: 481 PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541 APSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSHLR 600
           APSIE+TSSVDLDKNEAD+TSRTVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTSHLR
Sbjct: 541 APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601 ELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGTL 660
           ELVVTDL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G L
Sbjct: 601 ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGML 660

Query: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPILN 720
           QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKD RRKD+LLHIP LN
Sbjct: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLHIPTLN 720

Query: 721 VWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG 780
           +WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLS+AHG
Sbjct: 721 LWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSRAHG 780

Query: 781 VIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMH 840
           +IEMP PPPELLIFEPFIETDEI VSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSMH
Sbjct: 781 MIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSMH 840

Query: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQLEG 900
           SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELIA+ LQLEG
Sbjct: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEG 900

Query: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASERS 960
           FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ERS
Sbjct: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAWERS 960

Query: 961 P 962
           P
Sbjct: 961 P 960

BLAST of Spg033150 vs. NCBI nr
Match: XP_023528547.1 (uncharacterized protein LOC111791436 [Cucurbita pepo subsp. pepo] >XP_023528548.1 uncharacterized protein LOC111791436 [Cucurbita pepo subsp. pepo] >XP_023528549.1 uncharacterized protein LOC111791436 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1738.4 bits (4501), Expect = 0.0e+00
Identity = 880/961 (91.57%), Postives = 912/961 (94.90%), Query Frame = 0

Query: 1   MAYISNCSTCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHSRQLQSPEIRIPRTAAKL 60
           MA+I+NCSTCNV    +K RAS+F  TLSSSFL L+ D  +H+ +LQSP+IR+ R AAK+
Sbjct: 1   MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLGLSLDLHNHAPRLQSPKIRVSRAAAKM 60

Query: 61  VAKDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
           V KDGVAEMA TE+EREK S KC ALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61  VVKDGVAEMAATEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121 SCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIH 180
           SCYYID ELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLST VDIVFPVIH
Sbjct: 121 SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181 GRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVS 240
           GRFGEDGGIQELLE HNIPFVGTGSTEC HAFDKYNASLELDRLGFITV NFLVQAGSVS
Sbjct: 181 GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNASLELDRLGFITVPNFLVQAGSVS 240

Query: 241 ESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLV 300
           ES+LSKWF RNQLD SSGKVVVKPVRAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241 ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301 EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ 360
           EVFLE GSEFTAIVLDVGSGSVCHPVVLLPTEVELQ H WADAGEKDAIFNYRRKYLPTQ
Sbjct: 301 EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELQSHAWADAGEKDAIFNYRRKYLPTQ 360

Query: 361 QVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTE 420
           QVAYHTPPRFPI+VIESIREGASLLFKGLGL DFARIDGWFLPNSSH+ S STGKFGRT 
Sbjct: 361 QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNF 480
           SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 
Sbjct: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481 PRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL 540
           PRRSISSQR+Q YSS ES +KVFVI GGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCLL
Sbjct: 481 PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541 APSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSHLR 600
           APSIE+TSSVDLDKNEAD+TSRTVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTSHLR
Sbjct: 541 APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601 ELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGTL 660
           ELVVTDL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G L
Sbjct: 601 ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGML 660

Query: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPILN 720
           QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKD RRKD+LLHIP LN
Sbjct: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLHIPTLN 720

Query: 721 VWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG 780
           +WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLS+AHG
Sbjct: 721 LWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSRAHG 780

Query: 781 VIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMH 840
           +IEMP PPPELLIFEPFIETDEI VSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSMH
Sbjct: 781 MIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSMH 840

Query: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQLEG 900
           SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELIA+ LQLEG
Sbjct: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEG 900

Query: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASERS 960
           FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ERS
Sbjct: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLALERS 960

Query: 961 P 962
           P
Sbjct: 961 P 960

BLAST of Spg033150 vs. NCBI nr
Match: XP_022982716.1 (uncharacterized protein LOC111481502 [Cucurbita maxima] >XP_022982717.1 uncharacterized protein LOC111481502 [Cucurbita maxima] >XP_022982718.1 uncharacterized protein LOC111481502 [Cucurbita maxima])

HSP 1 Score: 1736.9 bits (4497), Expect = 0.0e+00
Identity = 878/961 (91.36%), Postives = 913/961 (95.01%), Query Frame = 0

Query: 1   MAYISNCSTCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHSRQLQSPEIRIPRTAAKL 60
           MA+ +NCSTC V    ++PRAS+F  TLSSSFLRL+ D  +H+R+LQSP+IR+ R AAK+
Sbjct: 1   MAHKANCSTCKVLCKDIEPRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60

Query: 61  VAKDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
           V KDGVAEMA TE+EREK S KC ALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61  VVKDGVAEMAATEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121 SCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIH 180
           SCYYID ELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLST VDIVFPVIH
Sbjct: 121 SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181 GRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVS 240
           GRFGEDGGIQELLE HNIPFVGTGSTEC HAFDKYNASLELDRLGFITV NFLVQAGSVS
Sbjct: 181 GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNASLELDRLGFITVPNFLVQAGSVS 240

Query: 241 ESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLV 300
           ES+LSKWF RNQLD SSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241 ESDLSKWFLRNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301 EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ 360
           EVFLE GSEFTAIVLDVGSGSVCHPVVLLPTEVELQ HGWADAGEKDAIFNYRRKYLPTQ
Sbjct: 301 EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELQSHGWADAGEKDAIFNYRRKYLPTQ 360

Query: 361 QVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTE 420
           QVAYHTPPRFPI+VIESIREGASLLFKGLGL DFARIDGWFLPNSSH+ S STGKFGRT 
Sbjct: 361 QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNF 480
           SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 
Sbjct: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481 PRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL 540
           PRRSISSQRSQ YSS ES +KVF+IFGGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCLL
Sbjct: 481 PRRSISSQRSQAYSSTESNQKVFLIFGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541 APSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSHLR 600
           APSIE+TSSVDLDKNEAD+TS+TVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTSHLR
Sbjct: 541 APSIEQTSSVDLDKNEADMTSKTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601 ELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGTL 660
           ELVVTDL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G L
Sbjct: 601 ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGML 660

Query: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPILN 720
           QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKD RRKD+LL+IP LN
Sbjct: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLNIPTLN 720

Query: 721 VWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG 780
           +WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVY KALE CLVRIPSNSLS+AHG
Sbjct: 721 LWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYAKALEYCLVRIPSNSLSRAHG 780

Query: 781 VIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMH 840
           +IEMP PPPELLIFEPFIETDEI VSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSMH
Sbjct: 781 MIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSMH 840

Query: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQLEG 900
           SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELIA+ALQLEG
Sbjct: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANALQLEG 900

Query: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASERS 960
           FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ERS
Sbjct: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLALERS 960

Query: 961 P 962
           P
Sbjct: 961 P 960

BLAST of Spg033150 vs. NCBI nr
Match: XP_038903726.1 (uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida] >XP_038903727.1 uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida] >XP_038903728.1 uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida] >XP_038903729.1 uncharacterized protein LOC120090245 isoform X1 [Benincasa hispida])

HSP 1 Score: 1733.4 bits (4488), Expect = 0.0e+00
Identity = 878/962 (91.27%), Postives = 923/962 (95.95%), Query Frame = 0

Query: 1   MAYISNCSTCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHS--RQLQSPEIRIPRTAA 60
           MAYI+NCSTC+VSS A+K RAS+FA TL SSFLRL+ D ++++  R+LQSP+I +PR+AA
Sbjct: 1   MAYITNCSTCDVSSKALKLRASTFAPTLPSSFLRLSLDLNNYTRRRRLQSPKIHLPRSAA 60

Query: 61  KLVAKDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDL 120
           K++AKDGVA+MAVT MERE+QS+KCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDL
Sbjct: 61  KVLAKDGVAQMAVTGMERERQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDL 120

Query: 121 QVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPV 180
           QVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGF SLT+FAEHLST VDIVFPV
Sbjct: 121 QVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFLSLTEFAEHLSTSVDIVFPV 180

Query: 181 IHGRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGS 240
           IHGRFGEDGGIQELLE HNIPFVGTGST+C HAFDKYNASLELDRLGFITV NFLVQAG 
Sbjct: 181 IHGRFGEDGGIQELLETHNIPFVGTGSTQCSHAFDKYNASLELDRLGFITVPNFLVQAGG 240

Query: 241 VSESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKV 300
           VSESELSKWF  NQLD SSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIIS EIDDKV
Sbjct: 241 VSESELSKWFLSNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISMEIDDKV 300

Query: 301 LVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLP 360
           LVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVE+Q  G ADAGEKDAIFNYRRKYLP
Sbjct: 301 LVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVEIQSLGLADAGEKDAIFNYRRKYLP 360

Query: 361 TQQVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGR 420
           TQQVAYHTPPRFPI+VIE+IREGASLLFKGLGL DFARIDGW+LPN SHE SCS GKFGR
Sbjct: 361 TQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPNFSHESSCSMGKFGR 420

Query: 421 TESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSG 480
           T+SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSG
Sbjct: 421 TKSGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSG 480

Query: 481 NFPRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPC 540
           N PRRSISSQRSQ YSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQ FDELEVTPC
Sbjct: 481 NTPRRSISSQRSQAYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQAFDELEVTPC 540

Query: 541 LLAPSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSH 600
           LLAPSIE+TS VDLDKNEA++T +TVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSH
Sbjct: 541 LLAPSIEQTSGVDLDKNEANLTFKTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSH 600

Query: 601 LRELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDG 660
           LRELVVTDL+EGLKKHSWF+GFDIKDELPVRFSLEQWIK+AKEVDATVFIAVHGGIGEDG
Sbjct: 601 LRELVVTDLEEGLKKHSWFSGFDIKDELPVRFSLEQWIKKAKEVDATVFIAVHGGIGEDG 660

Query: 661 TLQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPI 720
           TLQSLLEA+GVPYTGPGVAASNICMDKV+TSLALNHLS+MGVLTIKKD RRK+ LLHIPI
Sbjct: 661 TLQSLLEAQGVPYTGPGVAASNICMDKVSTSLALNHLSEMGVLTIKKDERRKEYLLHIPI 720

Query: 721 LNVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKA 780
           LNVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALE+CLVRIPSNSLSKA
Sbjct: 721 LNVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALENCLVRIPSNSLSKA 780

Query: 781 HGVIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGS 840
           HG+IEMP PPPELLIFEPFIETDEI VSSKST+E+ ERLLWKGQSRWVEITVGVVGTRGS
Sbjct: 781 HGMIEMPKPPPELLIFEPFIETDEIIVSSKSTDES-ERLLWKGQSRWVEITVGVVGTRGS 840

Query: 841 MHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQL 900
           MHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELIA+ALQL
Sbjct: 841 MHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANALQL 900

Query: 901 EGFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASE 960
           EGFSRIDAFV+VDSG+VLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA E
Sbjct: 901 EGFSRIDAFVDVDSGKVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLALE 960

BLAST of Spg033150 vs. NCBI nr
Match: XP_022157299.1 (uncharacterized protein LOC111024031 [Momordica charantia])

HSP 1 Score: 1726.1 bits (4469), Expect = 0.0e+00
Identity = 871/961 (90.63%), Postives = 904/961 (94.07%), Query Frame = 0

Query: 1   MAYISNCSTCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHSRQLQSPEIRIPRTAAKL 60
           MAYI+NCS CNV S  VKPRASSFA TLSSSFLR + DS+ H+ +L+ PEIR PR AAK+
Sbjct: 1   MAYIANCSNCNVLSKTVKPRASSFALTLSSSFLRSSVDSNHHTSRLRFPEIRFPRAAAKV 60

Query: 61  VAKDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
           VAK+GVAEMAVTE+ERE +    G LRVGLICGGPSAERGISLNSARSVLDHIQGDDL V
Sbjct: 61  VAKEGVAEMAVTELERENR----GVLRVGLICGGPSAERGISLNSARSVLDHIQGDDLLV 120

Query: 121 SCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIH 180
           SCYYID ELNAYAISSAQVYSNTPADFDFKLKSLA GF+SLTDFAEHLS  VDIVFPVIH
Sbjct: 121 SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLAHGFQSLTDFAEHLSASVDIVFPVIH 180

Query: 181 GRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVS 240
           GRFGEDGGIQELLERHNIPFVGTGSTEC HAFDKYNASLELD+LGFI V NFL+QAGSVS
Sbjct: 181 GRFGEDGGIQELLERHNIPFVGTGSTECSHAFDKYNASLELDKLGFIKVPNFLIQAGSVS 240

Query: 241 ESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLV 300
           ESELSKWF RNQ+D S GKVVVKPVRAGSSIGVTVAYGV DSLKKA EIIS+EIDDKVLV
Sbjct: 241 ESELSKWFLRNQIDPSFGKVVVKPVRAGSSIGVTVAYGVADSLKKAEEIISQEIDDKVLV 300

Query: 301 EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ 360
           EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ
Sbjct: 301 EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ 360

Query: 361 QVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTE 420
           QV YHTPPRF ++VIESIR+GASLLFKGLGLRDFARIDGWFLPNSS E SCS GKFGRTE
Sbjct: 361 QVTYHTPPRFAVDVIESIRKGASLLFKGLGLRDFARIDGWFLPNSSRESSCSVGKFGRTE 420

Query: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNF 480
           SGT+VYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYP LGSLDCMSGN 
Sbjct: 421 SGTIVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPDLGSLDCMSGNL 480

Query: 481 PRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL 540
            RRS S+QRSQ YSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL
Sbjct: 481 LRRSNSAQRSQAYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL 540

Query: 541 APSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSHLR 600
           APS E+TSS+DLDKN+ D TSRTVWSLPYSLVLRHTTEEVLAACIEA+EPTRAALTS LR
Sbjct: 541 APSNEQTSSLDLDKNQVDETSRTVWSLPYSLVLRHTTEEVLAACIEAMEPTRAALTSRLR 600

Query: 601 ELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGTL 660
           ELV+TDLKEGLK H WFAGFDIKDELPVR+SLEQWIKQAKE DATVFIAVHGGIGEDGTL
Sbjct: 601 ELVMTDLKEGLKNHRWFAGFDIKDELPVRYSLEQWIKQAKEADATVFIAVHGGIGEDGTL 660

Query: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPILN 720
           QSLLEAKGVPYTGPG AASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLL IPILN
Sbjct: 661 QSLLEAKGVPYTGPGAAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLQIPILN 720

Query: 721 VWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG 780
           VWHDLT KL CQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG
Sbjct: 721 VWHDLTRKLHCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG 780

Query: 781 VIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMH 840
           +IEMP PPPELLIFEPFIETDEI VSSKSTNET+E+LLW+GQSRWVEITVGVVGTRGSMH
Sbjct: 781 MIEMPKPPPELLIFEPFIETDEIIVSSKSTNETKEQLLWEGQSRWVEITVGVVGTRGSMH 840

Query: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQLEG 900
           SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPL II NEALEKCK+HIELIA+ LQLEG
Sbjct: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLMIIRNEALEKCKRHIELIANTLQLEG 900

Query: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASERS 960
           FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLL+LASERS
Sbjct: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLNLASERS 957

Query: 961 P 962
           P
Sbjct: 961 P 957

BLAST of Spg033150 vs. ExPASy Swiss-Prot
Match: Q0SNW3 (D-alanine--D-alanine ligase OS=Borrelia afzelii (strain PKo) OX=390236 GN=ddl PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 6.1e-23
Identity = 110/385 (28.57%), Postives = 168/385 (43.64%), Query Frame = 0

Query: 90  LICGGPSAERGISLNSARSV-LDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPADFD 149
           LI GG S E  IS  SA SV L  +  +   +   YID     + +  +      P D D
Sbjct: 7   LIFGGVSFEHEISCKSAYSVYLALLDLNKYNIYSAYIDKCTGIWYLLDSVSDPPKPIDTD 66

Query: 150 -FKLKSLARGFRSLTDFAEHLSTYVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTGSTE 209
              + SL  GF     F+ + +  +D+VFPV+HGR GEDG IQ +L+  +IP VG G   
Sbjct: 67  VLPIVSLLPGFGI---FSNNKNLEIDVVFPVVHGRTGEDGAIQGVLKVMDIPCVGAGIIG 126

Query: 210 CLHAFDKYNASLELD--RLGFITVHNFLVQAGSVSESELSKWFSRNQLDHSSGKVVVKPV 269
              + +KY   L L    +  +    F     S+ + E+     RN  +     V+VKP 
Sbjct: 127 SAISSNKYFCKLLLKSFNIPIVPFIGFRQYDYSLDKEEI----KRNVKEVLGYPVIVKPA 186

Query: 270 RAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCHP 329
             GSSIG+ VAY          E +  + D  +++E F+E      +I   +G+  +   
Sbjct: 187 VLGSSIGINVAYSENQIESCIEEAL--KYDLTIVIEKFIEAREIECSI---IGNEKM--- 246

Query: 330 VVLLPTEVELQFHGWADAGEKDAIF-NYRRKY--LPTQQVAYHTPPRFPIEVIESIREGA 389
            +  P EV +Q          D IF +Y  KY  +P   + ++ P       + SI+E A
Sbjct: 247 KIFSPGEVVVQ----------DFIFYDYDAKYSVIPGNSIIFNIPAHLETNQLLSIKEYA 306

Query: 390 SLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTESGTVVYTDINLISGMEQTSFLF 449
            L++K L LR  AR+D                 F   +SGT+   +IN I G    S   
Sbjct: 307 FLVYKNLELRGMARVD----------------FFVEKKSGTIYLNEINTIPGFTDISMFS 350

Query: 450 QQASKVGFSHSNILRSIIYHACLRY 468
           +  S  G    +++ ++I +A   Y
Sbjct: 367 KMCSHDGLQFKDLIDNLIDYAFQSY 350

BLAST of Spg033150 vs. ExPASy Swiss-Prot
Match: Q7NV72 (D-alanine--D-alanine ligase A OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) OX=243365 GN=ddlA PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 8.0e-23
Identity = 101/387 (26.10%), Postives = 148/387 (38.24%), Query Frame = 0

Query: 84  GALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNT 143
           G +RVGLI GG S+E  +SL SAR++L  I G+  +VS   +D +   +A  ++    N 
Sbjct: 2   GKIRVGLIFGGQSSEHEVSLQSARNILQAIDGERFEVSLIGVDKQGRWHASQASNFLLNA 61

Query: 144 PADFDFKLKSLARGFRSL---------TDFAEHLSTYVDIVFPVIHGRFGEDGGIQELLE 203
                  L+        +         T    H    +D+ FP++HG  GEDG +Q LL 
Sbjct: 62  DDPGRIALRESGENLALVPGECSGQLQTAANAHPLAQIDVAFPIVHGTLGEDGSLQGLLR 121

Query: 204 RHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVSESELSKWFSRNQLD 263
             NIPFVG G        DK  A   L   G        +     + ++LS    +  L 
Sbjct: 122 MANIPFVGAGVLGSAVCMDKDVAKRLLRDAGLKVAPFVSLTRSKAAGADLSAIVEQLGL- 181

Query: 264 HSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGS-EFTAI 323
                + VKP   GSS+GV+      D     +E      D KVLVE  + G   E   +
Sbjct: 182 ----PLFVKPANQGSSVGVSKVKREADLRAALDEAF--RYDHKVLVEQAVIGREIECAVL 241

Query: 324 VLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIE 383
             +    S C  +VL                  D  + Y  KYL         P   P E
Sbjct: 242 GNERPRASGCGEIVL-----------------SDEFYAYDTKYLNEDGARVAVPADIPDE 301

Query: 384 VIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTESGTVVYTDINLIS 443
             + IR  A   F+ L     AR+D +  P+                 G VV  ++N + 
Sbjct: 302 ACQRIRGIAIEAFQALECSGMARVDVFLTPD-----------------GEVVINELNTLP 347

Query: 444 GMEQTSFLFQQASKVGFSHSNILRSII 461
           G    S   +     G S+  ++ ++I
Sbjct: 362 GFTNISMYPKLWQAAGMSYRELITALI 347

BLAST of Spg033150 vs. ExPASy Swiss-Prot
Match: B8HAC4 (D-alanine--D-alanine ligase OS=Pseudarthrobacter chlorophenolicus (strain ATCC 700700 / DSM 12829 / CIP 107037 / JCM 12360 / KCTC 9906 / NCIMB 13794 / A6) OX=452863 GN=ddl PE=3 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 3.9e-22
Identity = 101/399 (25.31%), Postives = 171/399 (42.86%), Query Frame = 0

Query: 87  RVGLICGGPSAERGISLNSARSVLDHIQGDDLQV---------SCYYIDSELNAYAISSA 146
           RV ++ GG S+E  +S  +A  VL  I  D   V                +   ++++  
Sbjct: 18  RVAVLFGGRSSEHAVSCVTAAGVLGAINKDKYDVIPIGIAKSGQWVLAGGDTAQWSLAGK 77

Query: 147 QVYSNTPADFDFKLKSLARGFRSL----TDFAEHLSTYVDIVFPVIHGRFGEDGGIQELL 206
            +    P++    L  +    + +     +  + L T VD+VFP++HG FGEDG IQ LL
Sbjct: 78  ALPEVAPSEQTVTLAEIGGEHQLIVAAPNEVPQELGT-VDVVFPLLHGPFGEDGTIQGLL 137

Query: 207 ERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVSESELSKWFSRNQL 266
           E  +  +VG G        DK+   +  +  G   +H     A +  + +      R Q+
Sbjct: 138 ELSDTRYVGAGVLASAVGMDKHFMKVVFESAG---LHVGPYVAVTDRQWQKDPEAVRKQV 197

Query: 267 DHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEII--SKEIDDKVLVEVFLEGGSEFT 326
           D     V VKP RAGSS+G++     VDSL+  +  I  ++  D K+++E  +  G E  
Sbjct: 198 DRLGFPVFVKPARAGSSMGIS----KVDSLEGLDAAIEEARRHDLKLVIEAGIV-GREIE 257

Query: 327 AIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFP 386
             VL+ G G+   P   LP E+ +       AG     +++  KY+     +   P   P
Sbjct: 258 CAVLE-GRGTDA-PRTSLPGEISV-------AGGGHEFYDFNAKYVEDDAASLSCPADIP 317

Query: 387 IEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTESGTVVYTDINL 446
            E I  +RE A+  F  +G    +R+D ++ P+                 G ++  +IN 
Sbjct: 318 DEAIARVRELAAAAFDAVGAEGLSRVDFFYTPD-----------------GDLIINEINT 377

Query: 447 ISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSL 471
           + G    S   Q  +K G  ++ ++  +I+ A  R   L
Sbjct: 378 MPGFTPKSMYPQMWAKSGLGYAELIDELIHLALNRKTGL 381

BLAST of Spg033150 vs. ExPASy Swiss-Prot
Match: Q8PDW3 (D-alanine--D-alanine ligase A OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=ddlA PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 2.0e-21
Identity = 101/399 (25.31%), Postives = 158/399 (39.60%), Query Frame = 0

Query: 86  LRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPA 145
           +RVGLI GG SAE  +SL SAR++L  +     +     ID +   +  +      +   
Sbjct: 9   IRVGLIFGGKSAEHEVSLQSARNILQALDPQRFEPVLIGIDKQGQWHLSAQESFLLDADD 68

Query: 146 DFDFKLKSLARGFRSLTDF---------AEHLSTYVDIVFPVIHGRFGEDGGIQELLERH 205
                L+   RG   L            AE     +D+VFP++HG  GEDG +Q LL   
Sbjct: 69  PSRIALRHSGRGLAVLPGAASAQLRAVDAEQALAQIDVVFPIVHGTLGEDGSLQGLLRMA 128

Query: 206 NIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVSESELSKWFSRNQLDHS 265
           N+PFVG+G      A DK  A   L   G            + +++++    ++  L   
Sbjct: 129 NLPFVGSGVLGSAVAMDKDMAKRVLRDAGLEVAPFACFTRHTAAQADVQTLIAQFGL--- 188

Query: 266 SGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLD 325
              + VKP   GSS+GV+      +    A E ++   D KVLVE  +  G E    VL 
Sbjct: 189 --PLFVKPANQGSSVGVSQVRS-AEEFSAALE-LALRYDHKVLVEAAI-AGREIECAVLG 248

Query: 326 --VGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIEV 385
                 SVC  VV+                  DA ++Y+ KY+     A   P       
Sbjct: 249 NATPQASVCGEVVV-----------------HDAFYSYQTKYIRDDGAATVIPADIDAHT 308

Query: 386 IESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTESGTVVYTDINLISG 445
              I++ A   ++ L     AR+D          F C+ G+        +V  ++N + G
Sbjct: 309 QHRIQQCALQAYQALDCAGMARVD---------VFLCADGR--------IVINEVNTLPG 365

Query: 446 MEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSL 474
             + S   +     G  + +++  +I  A  R+   G L
Sbjct: 369 FTRISMYPKLWEASGLDYRSLVTRLIELALERHADDGRL 365

BLAST of Spg033150 vs. ExPASy Swiss-Prot
Match: Q9KCF0 (D-alanine--D-alanine ligase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=ddl PE=3 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 2.0e-21
Identity = 94/380 (24.74%), Postives = 160/380 (42.11%), Query Frame = 0

Query: 86  LRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTPA 145
           +++ ++ GG SAER +SL+S + +++ ++ +  +V                         
Sbjct: 1   MKIAVLYGGTSAEREVSLSSGKGIMEALKANGHEV------------------------I 60

Query: 146 DFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTGS 205
             DF    +    R L D        VD+VF  +HGR GEDG +Q LL+  NIP+VGTG 
Sbjct: 61  GIDFHPDQV----RDLVDLD------VDLVFIGLHGRLGEDGKVQALLDLLNIPYVGTGV 120

Query: 206 TECLHAFDKYNASLELDRLGF-----ITVHNFLVQAGSVSESELSKWFSRNQLDHSSGKV 265
                A DK  A L  ++ G      + +H+F   A + + +              +  V
Sbjct: 121 QGSALAMDKAKAKLFFEKAGIRVAEEVVLHSFTYDANAFNFT-------------GTYPV 180

Query: 266 VVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSG 325
           VVKP + GS+IG+TVA    + L+   E      DD +L+E F+ G     A++ + G  
Sbjct: 181 VVKPNQEGSTIGLTVAETEEELLQGIEEAFRH--DDTILIEEFIAGTEVTVAVLGNKGEE 240

Query: 326 SVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIEVIESIRE 385
                V ++P               K+ +++Y  KY P     +  P R   E    +++
Sbjct: 241 RSLPVVEIVP---------------KNKLYDYESKYAPGMS-EHIVPARISEEHTAYVQQ 299

Query: 386 GASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTESGTVVYTDINLISGMEQTSF 445
            A    + LG   ++R+D + +PN               +    V   +N + GM  TS 
Sbjct: 301 AAVRAHQALGCDVYSRVD-FIVPN---------------DGSDPVILKVNTLPGMTPTSL 299

Query: 446 LFQQASKVGFSHSNILRSII 461
               A  VG S+  ++++ +
Sbjct: 361 YPDAAKGVGMSYEEMIQTFV 299

BLAST of Spg033150 vs. ExPASy TrEMBL
Match: A0A6J1F3B4 (uncharacterized protein LOC111441803 OS=Cucurbita moschata OX=3662 GN=LOC111441803 PE=4 SV=1)

HSP 1 Score: 1741.1 bits (4508), Expect = 0.0e+00
Identity = 880/961 (91.57%), Postives = 914/961 (95.11%), Query Frame = 0

Query: 1   MAYISNCSTCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHSRQLQSPEIRIPRTAAKL 60
           MA+I+NCSTCNV    +K RAS+F  TLSSSFLRL+ D  +H+R+LQSP+IR+ R AAK+
Sbjct: 1   MAHIANCSTCNVLCKDIKLRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60

Query: 61  VAKDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
           V KDGVAEMA TE+EREK S KC ALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61  VVKDGVAEMATTEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121 SCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIH 180
           SCYYID ELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLST VDIVFPVIH
Sbjct: 121 SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181 GRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVS 240
           GRFGEDGGIQELLE HNIPFVGTGSTEC HAFDKYNA+LELDRLGFITV NFLVQAGSVS
Sbjct: 181 GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNAALELDRLGFITVPNFLVQAGSVS 240

Query: 241 ESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLV 300
           ES+LSKWF RNQLD SSGKVVVKPVRAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241 ESDLSKWFLRNQLDLSSGKVVVKPVRAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301 EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ 360
           EVFLE GSEFTAIVLDVGSGSVCHPVVLLPTEVEL+ H WADAGEKDAIFNYRRKYLPTQ
Sbjct: 301 EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELESHAWADAGEKDAIFNYRRKYLPTQ 360

Query: 361 QVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTE 420
           QVAYHTPPRFPI+VIESIREGASLLFKGLGL DFARIDGWFLPNSSH+ S STGKFGRT 
Sbjct: 361 QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNF 480
           SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 
Sbjct: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481 PRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL 540
           PRRSISSQR+Q YSS ES +KVFVI GGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCLL
Sbjct: 481 PRRSISSQRTQAYSSTESNQKVFVILGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541 APSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSHLR 600
           APSIE+TSSVDLDKNEAD+TSRTVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTSHLR
Sbjct: 541 APSIEQTSSVDLDKNEADMTSRTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601 ELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGTL 660
           ELVVTDL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G L
Sbjct: 601 ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGML 660

Query: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPILN 720
           QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKD RRKD+LLHIP LN
Sbjct: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLHIPTLN 720

Query: 721 VWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG 780
           +WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLS+AHG
Sbjct: 721 LWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSRAHG 780

Query: 781 VIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMH 840
           +IEMP PPPELLIFEPFIETDEI VSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSMH
Sbjct: 781 MIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSMH 840

Query: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQLEG 900
           SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELIA+ LQLEG
Sbjct: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANTLQLEG 900

Query: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASERS 960
           FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ERS
Sbjct: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAWERS 960

Query: 961 P 962
           P
Sbjct: 961 P 960

BLAST of Spg033150 vs. ExPASy TrEMBL
Match: A0A6J1IXB9 (uncharacterized protein LOC111481502 OS=Cucurbita maxima OX=3661 GN=LOC111481502 PE=4 SV=1)

HSP 1 Score: 1736.9 bits (4497), Expect = 0.0e+00
Identity = 878/961 (91.36%), Postives = 913/961 (95.01%), Query Frame = 0

Query: 1   MAYISNCSTCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHSRQLQSPEIRIPRTAAKL 60
           MA+ +NCSTC V    ++PRAS+F  TLSSSFLRL+ D  +H+R+LQSP+IR+ R AAK+
Sbjct: 1   MAHKANCSTCKVLCKDIEPRASNFTPTLSSSFLRLSLDLHNHARRLQSPKIRVSRAAAKM 60

Query: 61  VAKDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
           V KDGVAEMA TE+EREK S KC ALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV
Sbjct: 61  VVKDGVAEMAATEIEREKHSVKCEALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120

Query: 121 SCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIH 180
           SCYYID ELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLST VDIVFPVIH
Sbjct: 121 SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFPVIH 180

Query: 181 GRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVS 240
           GRFGEDGGIQELLE HNIPFVGTGSTEC HAFDKYNASLELDRLGFITV NFLVQAGSVS
Sbjct: 181 GRFGEDGGIQELLETHNIPFVGTGSTECAHAFDKYNASLELDRLGFITVPNFLVQAGSVS 240

Query: 241 ESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLV 300
           ES+LSKWF RNQLD SSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIISKEIDDKVLV
Sbjct: 241 ESDLSKWFLRNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISKEIDDKVLV 300

Query: 301 EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ 360
           EVFLE GSEFTAIVLDVGSGSVCHPVVLLPTEVELQ HGWADAGEKDAIFNYRRKYLPTQ
Sbjct: 301 EVFLERGSEFTAIVLDVGSGSVCHPVVLLPTEVELQSHGWADAGEKDAIFNYRRKYLPTQ 360

Query: 361 QVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTE 420
           QVAYHTPPRFPI+VIESIREGASLLFKGLGL DFARIDGWFLPNSSH+ S STGKFGRT 
Sbjct: 361 QVAYHTPPRFPIDVIESIREGASLLFKGLGLCDFARIDGWFLPNSSHDSSSSTGKFGRTA 420

Query: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNF 480
           SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGN 
Sbjct: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNM 480

Query: 481 PRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL 540
           PRRSISSQRSQ YSS ES +KVF+IFGGDTSERQVSLMSGTNVWLNLQ FD+LEVTPCLL
Sbjct: 481 PRRSISSQRSQAYSSTESNQKVFLIFGGDTSERQVSLMSGTNVWLNLQAFDDLEVTPCLL 540

Query: 541 APSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSHLR 600
           APSIE+TSSVDLDKNEAD+TS+TVWSLPYSLVLRHTTEEVLAACIEA EPTRAALTSHLR
Sbjct: 541 APSIEQTSSVDLDKNEADMTSKTVWSLPYSLVLRHTTEEVLAACIEAFEPTRAALTSHLR 600

Query: 601 ELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGTL 660
           ELVVTDL+EGLKKHSWFAGFDIKDELPV+FSLEQWIK+AKEVDATVFIAVHGGIGE+G L
Sbjct: 601 ELVVTDLEEGLKKHSWFAGFDIKDELPVKFSLEQWIKKAKEVDATVFIAVHGGIGENGML 660

Query: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPILN 720
           QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKD RRKD+LL+IP LN
Sbjct: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDARRKDELLNIPTLN 720

Query: 721 VWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG 780
           +WHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVY KALE CLVRIPSNSLS+AHG
Sbjct: 721 LWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYAKALEYCLVRIPSNSLSRAHG 780

Query: 781 VIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMH 840
           +IEMP PPPELLIFEPFIETDEI VSSK TNE+ ERLLWKGQSRWVEITVGVVGTRGSMH
Sbjct: 781 MIEMPKPPPELLIFEPFIETDEIIVSSKYTNES-ERLLWKGQSRWVEITVGVVGTRGSMH 840

Query: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQLEG 900
           SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNE+LEKCKQHIELIA+ALQLEG
Sbjct: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLEKCKQHIELIANALQLEG 900

Query: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASERS 960
           FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA ERS
Sbjct: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLALERS 960

Query: 961 P 962
           P
Sbjct: 961 P 960

BLAST of Spg033150 vs. ExPASy TrEMBL
Match: A0A6J1DW40 (uncharacterized protein LOC111024031 OS=Momordica charantia OX=3673 GN=LOC111024031 PE=4 SV=1)

HSP 1 Score: 1726.1 bits (4469), Expect = 0.0e+00
Identity = 871/961 (90.63%), Postives = 904/961 (94.07%), Query Frame = 0

Query: 1   MAYISNCSTCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHSRQLQSPEIRIPRTAAKL 60
           MAYI+NCS CNV S  VKPRASSFA TLSSSFLR + DS+ H+ +L+ PEIR PR AAK+
Sbjct: 1   MAYIANCSNCNVLSKTVKPRASSFALTLSSSFLRSSVDSNHHTSRLRFPEIRFPRAAAKV 60

Query: 61  VAKDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQV 120
           VAK+GVAEMAVTE+ERE +    G LRVGLICGGPSAERGISLNSARSVLDHIQGDDL V
Sbjct: 61  VAKEGVAEMAVTELERENR----GVLRVGLICGGPSAERGISLNSARSVLDHIQGDDLLV 120

Query: 121 SCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIH 180
           SCYYID ELNAYAISSAQVYSNTPADFDFKLKSLA GF+SLTDFAEHLS  VDIVFPVIH
Sbjct: 121 SCYYIDPELNAYAISSAQVYSNTPADFDFKLKSLAHGFQSLTDFAEHLSASVDIVFPVIH 180

Query: 181 GRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAGSVS 240
           GRFGEDGGIQELLERHNIPFVGTGSTEC HAFDKYNASLELD+LGFI V NFL+QAGSVS
Sbjct: 181 GRFGEDGGIQELLERHNIPFVGTGSTECSHAFDKYNASLELDKLGFIKVPNFLIQAGSVS 240

Query: 241 ESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLV 300
           ESELSKWF RNQ+D S GKVVVKPVRAGSSIGVTVAYGV DSLKKA EIIS+EIDDKVLV
Sbjct: 241 ESELSKWFLRNQIDPSFGKVVVKPVRAGSSIGVTVAYGVADSLKKAEEIISQEIDDKVLV 300

Query: 301 EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ 360
           EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ
Sbjct: 301 EVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQ 360

Query: 361 QVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTE 420
           QV YHTPPRF ++VIESIR+GASLLFKGLGLRDFARIDGWFLPNSS E SCS GKFGRTE
Sbjct: 361 QVTYHTPPRFAVDVIESIRKGASLLFKGLGLRDFARIDGWFLPNSSRESSCSVGKFGRTE 420

Query: 421 SGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNF 480
           SGT+VYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYP LGSLDCMSGN 
Sbjct: 421 SGTIVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPDLGSLDCMSGNL 480

Query: 481 PRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL 540
            RRS S+QRSQ YSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL
Sbjct: 481 LRRSNSAQRSQAYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLL 540

Query: 541 APSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSHLR 600
           APS E+TSS+DLDKN+ D TSRTVWSLPYSLVLRHTTEEVLAACIEA+EPTRAALTS LR
Sbjct: 541 APSNEQTSSLDLDKNQVDETSRTVWSLPYSLVLRHTTEEVLAACIEAMEPTRAALTSRLR 600

Query: 601 ELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGTL 660
           ELV+TDLKEGLK H WFAGFDIKDELPVR+SLEQWIKQAKE DATVFIAVHGGIGEDGTL
Sbjct: 601 ELVMTDLKEGLKNHRWFAGFDIKDELPVRYSLEQWIKQAKEADATVFIAVHGGIGEDGTL 660

Query: 661 QSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPILN 720
           QSLLEAKGVPYTGPG AASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLL IPILN
Sbjct: 661 QSLLEAKGVPYTGPGAAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLQIPILN 720

Query: 721 VWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG 780
           VWHDLT KL CQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG
Sbjct: 721 VWHDLTRKLHCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHG 780

Query: 781 VIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMH 840
           +IEMP PPPELLIFEPFIETDEI VSSKSTNET+E+LLW+GQSRWVEITVGVVGTRGSMH
Sbjct: 781 MIEMPKPPPELLIFEPFIETDEIIVSSKSTNETKEQLLWEGQSRWVEITVGVVGTRGSMH 840

Query: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQLEG 900
           SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPL II NEALEKCK+HIELIA+ LQLEG
Sbjct: 841 SLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLMIIRNEALEKCKRHIELIANTLQLEG 900

Query: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLASERS 960
           FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLL+LASERS
Sbjct: 901 FSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLNLASERS 957

Query: 961 P 962
           P
Sbjct: 961 P 957

BLAST of Spg033150 vs. ExPASy TrEMBL
Match: A0A5A7TLK1 (D-alanine--D-alanine ligase family protein isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005710 PE=4 SV=1)

HSP 1 Score: 1698.7 bits (4398), Expect = 0.0e+00
Identity = 867/963 (90.03%), Postives = 908/963 (94.29%), Query Frame = 0

Query: 1   MAYISNC--STCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHSRQLQSPEIRIPRTAA 60
           MAYI+NC  STC+  S+ +K   S+FA TLSSSFLRL+  S++H+  L SP+ ++PR+AA
Sbjct: 1   MAYIANCSYSTCHPLSDVLKLTPSTFAPTLSSSFLRLSLQSNNHTPPLHSPKFQLPRSAA 60

Query: 61  KLVA-KDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDD 120
           K++A KD VAEMAVT  EREKQS+KCGALRVGLICGGPSAERGISLNSARSVLDHIQG D
Sbjct: 61  KVLANKDVVAEMAVTRTEREKQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGHD 120

Query: 121 LQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFP 180
           LQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLST VDIVFP
Sbjct: 121 LQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP 180

Query: 181 VIHGRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAG 240
           VIHGRFGEDGGIQELLE HNIPFVGTGSTE   AFDKY+ASLELDRLGFITV NFLVQAG
Sbjct: 181 VIHGRFGEDGGIQELLETHNIPFVGTGSTESSRAFDKYDASLELDRLGFITVPNFLVQAG 240

Query: 241 SVSESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDK 300
           SVSE+ELSKWF  NQLD SSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIIS+EIDDK
Sbjct: 241 SVSETELSKWFVSNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISEEIDDK 300

Query: 301 VLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYL 360
           VLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVE+Q    ADAGEKDAIFNYRRKYL
Sbjct: 301 VLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVEIQSRDVADAGEKDAIFNYRRKYL 360

Query: 361 PTQQVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFG 420
           PTQQVAYHTPPRFPI+VIE+IREGASLLFKGLGL DFARIDGW+LP+ SHE SCSTGKFG
Sbjct: 361 PTQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPSFSHESSCSTGKFG 420

Query: 421 RTESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMS 480
           +TESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRY SLGSLDC+S
Sbjct: 421 KTESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYSSLGSLDCIS 480

Query: 481 GNFPRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTP 540
           GN PRRSISSQRSQ YS+HESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQ FDELEVTP
Sbjct: 481 GNVPRRSISSQRSQAYSTHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQAFDELEVTP 540

Query: 541 CLLAPSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 600
           CLLAPSIE+ SSVDLDKNEAD+TSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS
Sbjct: 541 CLLAPSIEQASSVDLDKNEADLTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 600

Query: 601 HLRELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGED 660
           HLRELVVTDL+EGLKKHSWFAGFDIKDELPVRFSLEQWI++AKEVDATVFIAVHGGIGED
Sbjct: 601 HLRELVVTDLEEGLKKHSWFAGFDIKDELPVRFSLEQWIEKAKEVDATVFIAVHGGIGED 660

Query: 661 GTLQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIP 720
           GTLQSLLEAKGVPYTGPGVAASNICMDKV+TSLALNHLSD GVLT KKDVR+KDDLL  P
Sbjct: 661 GTLQSLLEAKGVPYTGPGVAASNICMDKVSTSLALNHLSDFGVLTTKKDVRKKDDLLRFP 720

Query: 721 ILNVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSK 780
           ILNVW DLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSK
Sbjct: 721 ILNVWRDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSK 780

Query: 781 AHGVIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRG 840
           AHG+IEMP PPPELLIFEPFIETDEI VSSK T +  ERLLWKGQSRWVEITVGVVGTRG
Sbjct: 781 AHGMIEMPKPPPELLIFEPFIETDEIIVSSK-TIDASERLLWKGQSRWVEITVGVVGTRG 840

Query: 841 SMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQ 900
           SMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNE+L KCKQHIELIA+ALQ
Sbjct: 841 SMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLGKCKQHIELIANALQ 900

Query: 901 LEGFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAS 960
           LEGFSRIDAFV+VDSG+VLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA 
Sbjct: 901 LEGFSRIDAFVDVDSGKVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAL 960

BLAST of Spg033150 vs. ExPASy TrEMBL
Match: A0A1S3B5H4 (uncharacterized protein LOC103486245 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486245 PE=4 SV=1)

HSP 1 Score: 1698.7 bits (4398), Expect = 0.0e+00
Identity = 867/963 (90.03%), Postives = 908/963 (94.29%), Query Frame = 0

Query: 1   MAYISNC--STCNVSSNAVKPRASSFARTLSSSFLRLAPDSSSHSRQLQSPEIRIPRTAA 60
           MAYI+NC  STC+  S+ +K   S+FA TLSSSFLRL+  S++H+  L SP+ ++PR+AA
Sbjct: 1   MAYIANCSYSTCHPLSDVLKLTPSTFAPTLSSSFLRLSLQSNNHTPPLHSPKFQLPRSAA 60

Query: 61  KLVA-KDGVAEMAVTEMEREKQSDKCGALRVGLICGGPSAERGISLNSARSVLDHIQGDD 120
           K++A KD VAEMAVT  EREKQS+KCGALRVGLICGGPSAERGISLNSARSVLDHIQG D
Sbjct: 61  KVLANKDVVAEMAVTRTEREKQSEKCGALRVGLICGGPSAERGISLNSARSVLDHIQGHD 120

Query: 121 LQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFP 180
           LQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLST VDIVFP
Sbjct: 121 LQVSCYYIDSELNAYAISSAQVYSNTPADFDFKLKSLARGFRSLTDFAEHLSTSVDIVFP 180

Query: 181 VIHGRFGEDGGIQELLERHNIPFVGTGSTECLHAFDKYNASLELDRLGFITVHNFLVQAG 240
           VIHGRFGEDGGIQELLE HNIPFVGTGSTE   AFDKY+ASLELDRLGFITV NFLVQAG
Sbjct: 181 VIHGRFGEDGGIQELLETHNIPFVGTGSTESSRAFDKYDASLELDRLGFITVPNFLVQAG 240

Query: 241 SVSESELSKWFSRNQLDHSSGKVVVKPVRAGSSIGVTVAYGVVDSLKKANEIISKEIDDK 300
           SVSE+ELSKWF  NQLD SSGKVVVKP RAGSSIGV+VAYGVVDSLKKANEIIS+EIDDK
Sbjct: 241 SVSETELSKWFVSNQLDLSSGKVVVKPARAGSSIGVSVAYGVVDSLKKANEIISEEIDDK 300

Query: 301 VLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVELQFHGWADAGEKDAIFNYRRKYL 360
           VLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVE+Q    ADAGEKDAIFNYRRKYL
Sbjct: 301 VLVEVFLEGGSEFTAIVLDVGSGSVCHPVVLLPTEVEIQSRDVADAGEKDAIFNYRRKYL 360

Query: 361 PTQQVAYHTPPRFPIEVIESIREGASLLFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFG 420
           PTQQVAYHTPPRFPI+VIE+IREGASLLFKGLGL DFARIDGW+LP+ SHE SCSTGKFG
Sbjct: 361 PTQQVAYHTPPRFPIDVIETIREGASLLFKGLGLCDFARIDGWYLPSFSHESSCSTGKFG 420

Query: 421 RTESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCMS 480
           +TESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDC+S
Sbjct: 421 KTESGTVVYTDINLISGMEQTSFLFQQASKVGFSHSNILRSIIYHACLRYPSLGSLDCIS 480

Query: 481 GNFPRRSISSQRSQVYSSHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQVFDELEVTP 540
           GN PRRSISSQ SQ YS+HESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQ FDELEVTP
Sbjct: 481 GNVPRRSISSQCSQAYSTHESIRKVFVIFGGDTSERQVSLMSGTNVWLNLQAFDELEVTP 540

Query: 541 CLLAPSIEKTSSVDLDKNEADVTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 600
           CLLAPSIE+ SSVDLDKNEAD+TSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS
Sbjct: 541 CLLAPSIEQASSVDLDKNEADLTSRTVWSLPYSLVLRHTTEEVLAACIEAIEPTRAALTS 600

Query: 601 HLRELVVTDLKEGLKKHSWFAGFDIKDELPVRFSLEQWIKQAKEVDATVFIAVHGGIGED 660
           HLRELVV DL+EGLKKHSWFAGFDIKDELPVRFSLEQWI++AKEVDATVFIAVHGGIGED
Sbjct: 601 HLRELVVNDLEEGLKKHSWFAGFDIKDELPVRFSLEQWIEKAKEVDATVFIAVHGGIGED 660

Query: 661 GTLQSLLEAKGVPYTGPGVAASNICMDKVATSLALNHLSDMGVLTIKKDVRRKDDLLHIP 720
           GTLQSLLEAKGVPYTGPGVAASNICMDKV+TSLALNHLSD GVLTIKKDVR+KDDLL  P
Sbjct: 661 GTLQSLLEAKGVPYTGPGVAASNICMDKVSTSLALNHLSDFGVLTIKKDVRKKDDLLRFP 720

Query: 721 ILNVWHDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSK 780
           ILNVW DLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSK
Sbjct: 721 ILNVWRDLTCKLQCQSLCVKPARDGCSTGVARLCCADDLAVYVKALEDCLVRIPSNSLSK 780

Query: 781 AHGVIEMPNPPPELLIFEPFIETDEITVSSKSTNETEERLLWKGQSRWVEITVGVVGTRG 840
           AHG+IEMP PPPELLIFEPFIETDEI VSSK T +  ERLLWKGQSRWVEITVGVVGTRG
Sbjct: 781 AHGMIEMPKPPPELLIFEPFIETDEIIVSSK-TIDASERLLWKGQSRWVEITVGVVGTRG 840

Query: 841 SMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNEALEKCKQHIELIASALQ 900
           SMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNE+L KCKQHIELIA+ALQ
Sbjct: 841 SMHSLSPSVTVKESGDILSLEEKFQGGTGINLTPPPLSIISNESLGKCKQHIELIANALQ 900

Query: 901 LEGFSRIDAFVNVDSGEVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAS 960
           LEGFSRIDAFV+VDSG+VLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLA 
Sbjct: 901 LEGFSRIDAFVDVDSGKVLVIEVNTVPGMTPSTVLIHQALAETPPVYPHQFFRRLLDLAL 960

BLAST of Spg033150 vs. TAIR 10
Match: AT3G08840.2 (D-alanine--D-alanine ligase family )

HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 613/875 (70.06%), Postives = 718/875 (82.06%), Query Frame = 0

Query: 85  ALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTP 144
           +LRVGLICGGPSAERGISLNSARSVLDHIQGD + VSCYYID +L A+AISSAQVYSNTP
Sbjct: 77  SLRVGLICGGPSAERGISLNSARSVLDHIQGDGINVSCYYIDPDLKAFAISSAQVYSNTP 136

Query: 145 ADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTG 204
           +DFDFKL+SLA+GF SL++ AEHL + VDIVFPVIHGRFGEDGGIQELLE HNIPFVGTG
Sbjct: 137 SDFDFKLESLAQGFSSLSELAEHLVSAVDIVFPVIHGRFGEDGGIQELLESHNIPFVGTG 196

Query: 205 STECLHAFDKYNASLELDRLGFITVHNFLVQAGSVSESELSKWFSRNQLDHSSGKVVVKP 264
           S EC  AFDKY ASLEL  LGF+TV N+LVQ   V +SE++ WF+ NQLD   GKVVVKP
Sbjct: 197 SRECFRAFDKYEASLELKELGFMTVPNYLVQGTGVDKSEIALWFTDNQLDLEMGKVVVKP 256

Query: 265 VRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCH 324
            +AGSSIGV VA+GV DS+KKA E+I + IDD+V+VEVF+E   EFTAIVLDVGSGSVCH
Sbjct: 257 AKAGSSIGVKVAFGVNDSIKKATELILEGIDDRVVVEVFIEDAYEFTAIVLDVGSGSVCH 316

Query: 325 PVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIEVIESIREGASL 384
           PVVL+PTEV+LQFHG  D  E +AIF+YRRKYLPTQQV YHTPPRFPI VI+SIRE ASL
Sbjct: 317 PVVLMPTEVQLQFHGIGDPKE-NAIFDYRRKYLPTQQVTYHTPPRFPIHVIKSIREEASL 376

Query: 385 LFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTESGTVVYTDINLISGMEQTSFLFQQ 444
           +F+ LGLRDFARIDGW+L  +S+  S  +   G T+SG +++TDINLISGMEQTSFLFQQ
Sbjct: 377 IFQKLGLRDFARIDGWYLAPNSNLSSPVSETLGGTKSGDIIFTDINLISGMEQTSFLFQQ 436

Query: 445 ASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNFPRRSISSQRSQVYSSHESIRKVFV 504
           ASKVGFSHSNILR+I++ AC R+P    LD  +  +   S   Q S      E  +KVFV
Sbjct: 437 ASKVGFSHSNILRTIVHRACSRFP---HLDWYNYGY---SQLLQGSTTLEVSEDPQKVFV 496

Query: 505 IFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAPSIEKTSSVDLDKNEADVTSRTV 564
           IFGGDTSERQVS+MSGTNVW+NLQ + +L VTPCLL+PS+  +         +++ +R V
Sbjct: 497 IFGGDTSERQVSVMSGTNVWVNLQRYVDLNVTPCLLSPSLSNSLGA-----SSNLDNREV 556

Query: 565 WSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSHLRELVVTDLKEGLKKHSWFAGFDIKD 624
           W LPYS+VLRHT EEVLAAC+EA+EP RA  TS L++ V+ DL +G K  SWFAGFDI D
Sbjct: 557 WVLPYSVVLRHTAEEVLAACLEAVEPVRALFTSLLQKQVMEDLMDGFKNQSWFAGFDITD 616

Query: 625 ELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGTLQSLLEAKGVPYTGPGVAASNICMD 684
           ELP ++SL++WIK AKE  ATVFIAVHGGIGEDGTLQ LLE +GV YTGPGV AS  CMD
Sbjct: 617 ELPRKYSLKEWIKHAKEAQATVFIAVHGGIGEDGTLQGLLEDEGVSYTGPGVLASRTCMD 676

Query: 685 KVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPILNVWHDLTCKLQCQSLCVKPARDGCS 744
           KV TS AL++LS+ G+ TI KDV+R +D++H    NVW +L  KLQC +LCVKPA+DGCS
Sbjct: 677 KVMTSQALSNLSEFGIHTISKDVKRTEDIMHETFPNVWDELIKKLQCLTLCVKPAKDGCS 736

Query: 745 TGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHGVIEMPNPPPELLIFEPFIETDEIT 804
           TGVARLC ++DLAVYV+AL+DC+ RIP N+LSK HG+IEMPNP PE LIFEPF+ETDEI 
Sbjct: 737 TGVARLCSSEDLAVYVQALKDCIPRIPPNTLSKTHGMIEMPNPTPEFLIFEPFVETDEII 796

Query: 805 VSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMHSLSPSVTVKESGDILSLEEKFQGG 864
           VSSK+    +++L WKG+ RWVE+TVGV+G RGSMHSLSPS+TVKESGDILSLEEKFQGG
Sbjct: 797 VSSKA----KQQLSWKGRRRWVEMTVGVIGKRGSMHSLSPSLTVKESGDILSLEEKFQGG 856

Query: 865 TGINLTPPPLSIISNEALEKCKQHIELIASALQLEGFSRIDAFVNVDSGEVLVIEVNTVP 924
           TGINLTPPP +I+S EALE+CKQ IELIA  L LEGFSRIDAFV+V++GEVLVIEVNTVP
Sbjct: 857 TGINLTPPPPTIMSKEALERCKQGIELIAETLGLEGFSRIDAFVHVETGEVLVIEVNTVP 916

Query: 925 GMTPSTVLIHQALAETPPVYPHQFFRRLLDLASER 960
           GMTPSTVLI QALAE PP+YP QFFR LL LA++R
Sbjct: 917 GMTPSTVLIQQALAEQPPMYPPQFFRTLLHLATQR 935

BLAST of Spg033150 vs. TAIR 10
Match: AT3G08840.3 (D-alanine--D-alanine ligase family )

HSP 1 Score: 1062.4 bits (2746), Expect = 2.2e-310
Identity = 536/778 (68.89%), Postives = 632/778 (81.23%), Query Frame = 0

Query: 85  ALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTP 144
           +LRVGLICGGPSAERGISLNSARSVLDHIQGD + VSCYYID +L A+AISSAQVYSNTP
Sbjct: 77  SLRVGLICGGPSAERGISLNSARSVLDHIQGDGINVSCYYIDPDLKAFAISSAQVYSNTP 136

Query: 145 ADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTG 204
           +DFDFKL+SLA+GF SL++ AEHL + VDIVFPVIHGRFGEDGGIQELLE HNIPFVGTG
Sbjct: 137 SDFDFKLESLAQGFSSLSELAEHLVSAVDIVFPVIHGRFGEDGGIQELLESHNIPFVGTG 196

Query: 205 STECLHAFDKYNASLELDRLGFITVHNFLVQAGSVSESELSKWFSRNQLDHSSGKVVVKP 264
           S EC  AFDKY ASLEL  LGF+TV N+LVQ   V +SE++ WF+ NQLD   GKVVVKP
Sbjct: 197 SRECFRAFDKYEASLELKELGFMTVPNYLVQGTGVDKSEIALWFTDNQLDLEMGKVVVKP 256

Query: 265 VRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCH 324
            +AGSSIGV VA+GV DS+KKA E+I + IDD+V+VEVF+E   EFTAIVLDVGSGSVCH
Sbjct: 257 AKAGSSIGVKVAFGVNDSIKKATELILEGIDDRVVVEVFIEDAYEFTAIVLDVGSGSVCH 316

Query: 325 PVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIEVIESIREGASL 384
           PVVL+PTEV+LQFHG  D  E +AIF+YRRKYLPTQQV YHTPPRFPI VI+SIRE ASL
Sbjct: 317 PVVLMPTEVQLQFHGIGDPKE-NAIFDYRRKYLPTQQVTYHTPPRFPIHVIKSIREEASL 376

Query: 385 LFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTESGTVVYTDINLISGMEQTSFLFQQ 444
           +F+ LGLRDFARIDGW+L  +S+  S  +   G T+SG +++TDINLISGMEQTSFLFQQ
Sbjct: 377 IFQKLGLRDFARIDGWYLAPNSNLSSPVSETLGGTKSGDIIFTDINLISGMEQTSFLFQQ 436

Query: 445 ASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNFPRRSISSQRSQVYSSHESIRKVFV 504
           ASKVGFSHSNILR+I++ AC R+P    LD  +  +   S   Q S      E  +KVFV
Sbjct: 437 ASKVGFSHSNILRTIVHRACSRFP---HLDWYNYGY---SQLLQGSTTLEVSEDPQKVFV 496

Query: 505 IFGGDTSERQVSLMSGTNVWLNLQVFDELEVTPCLLAPSIEKTSSVDLDKNEADVTSRTV 564
           IFGGDTSERQVS+MSGTNVW+NLQ + +L VTPCLL+PS+  +         +++ +R V
Sbjct: 497 IFGGDTSERQVSVMSGTNVWVNLQRYVDLNVTPCLLSPSLSNSLGA-----SSNLDNREV 556

Query: 565 WSLPYSLVLRHTTEEVLAACIEAIEPTRAALTSHLRELVVTDLKEGLKKHSWFAGFDIKD 624
           W LPYS+VLRHT EEVLAAC+EA+EP RA  TS L++ V+ DL +G K  SWFAGFDI D
Sbjct: 557 WVLPYSVVLRHTAEEVLAACLEAVEPVRALFTSLLQKQVMEDLMDGFKNQSWFAGFDITD 616

Query: 625 ELPVRFSLEQWIKQAKEVDATVFIAVHGGIGEDGTLQSLLEAKGVPYTGPGVAASNICMD 684
           ELP ++SL++WIK AKE  ATVFIAVHGGIGEDGTLQ LLE +GV YTGPGV AS  CMD
Sbjct: 617 ELPRKYSLKEWIKHAKEAQATVFIAVHGGIGEDGTLQGLLEDEGVSYTGPGVLASRTCMD 676

Query: 685 KVATSLALNHLSDMGVLTIKKDVRRKDDLLHIPILNVWHDLTCKLQCQSLCVKPARDGCS 744
           KV TS AL++LS+ G+ TI KDV+R +D++H    NVW +L  KLQC +LCVKPA+DGCS
Sbjct: 677 KVMTSQALSNLSEFGIHTISKDVKRTEDIMHETFPNVWDELIKKLQCLTLCVKPAKDGCS 736

Query: 745 TGVARLCCADDLAVYVKALEDCLVRIPSNSLSKAHGVIEMPNPPPELLIFEPFIETDEIT 804
           TGVARLC ++DLAVYV+AL+DC+ RIP N+LSK HG+IEMPNP PE LIFEPF+ETDEI 
Sbjct: 737 TGVARLCSSEDLAVYVQALKDCIPRIPPNTLSKTHGMIEMPNPTPEFLIFEPFVETDEII 796

Query: 805 VSSKSTNETEERLLWKGQSRWVEITVGVVGTRGSMHSLSPSVTVKESGDILSLEEKFQ 863
           VSSK+    +++L WKG+ RWVE+TVGV+G RGSMHSLSPS+TVKESGDILSLEEKFQ
Sbjct: 797 VSSKA----KQQLSWKGRRRWVEMTVGVIGKRGSMHSLSPSLTVKESGDILSLEEKFQ 838

BLAST of Spg033150 vs. TAIR 10
Match: AT3G08840.1 (D-alanine--D-alanine ligase family )

HSP 1 Score: 620.2 bits (1598), Expect = 2.9e-177
Identity = 317/444 (71.40%), Postives = 365/444 (82.21%), Query Frame = 0

Query: 85  ALRVGLICGGPSAERGISLNSARSVLDHIQGDDLQVSCYYIDSELNAYAISSAQVYSNTP 144
           +LRVGLICGGPSAERGISLNSARSVLDHIQGD + VSCYYID +L A+AISSAQVYSNTP
Sbjct: 77  SLRVGLICGGPSAERGISLNSARSVLDHIQGDGINVSCYYIDPDLKAFAISSAQVYSNTP 136

Query: 145 ADFDFKLKSLARGFRSLTDFAEHLSTYVDIVFPVIHGRFGEDGGIQELLERHNIPFVGTG 204
           +DFDFKL+SLA+GF SL++ AEHL + VDIVFPVIHGRFGEDGGIQELLE HNIPFVGTG
Sbjct: 137 SDFDFKLESLAQGFSSLSELAEHLVSAVDIVFPVIHGRFGEDGGIQELLESHNIPFVGTG 196

Query: 205 STECLHAFDKYNASLELDRLGFITVHNFLVQAGSVSESELSKWFSRNQLDHSSGKVVVKP 264
           S EC  AFDKY ASLEL  LGF+TV N+LVQ   V +SE++ WF+ NQLD   GKVVVKP
Sbjct: 197 SRECFRAFDKYEASLELKELGFMTVPNYLVQGTGVDKSEIALWFTDNQLDLEMGKVVVKP 256

Query: 265 VRAGSSIGVTVAYGVVDSLKKANEIISKEIDDKVLVEVFLEGGSEFTAIVLDVGSGSVCH 324
            +AGSSIGV VA+GV DS+KKA E+I + IDD+V+VEVF+E   EFTAIVLDVGSGSVCH
Sbjct: 257 AKAGSSIGVKVAFGVNDSIKKATELILEGIDDRVVVEVFIEDAYEFTAIVLDVGSGSVCH 316

Query: 325 PVVLLPTEVELQFHGWADAGEKDAIFNYRRKYLPTQQVAYHTPPRFPIEVIESIREGASL 384
           PVVL+PTEV+LQFHG  D  E +AIF+YRRKYLPTQQV YHTPPRFPI VI+SIRE ASL
Sbjct: 317 PVVLMPTEVQLQFHGIGDPKE-NAIFDYRRKYLPTQQVTYHTPPRFPIHVIKSIREEASL 376

Query: 385 LFKGLGLRDFARIDGWFLPNSSHEFSCSTGKFGRTESGTVVYTDINLISGMEQTSFLFQQ 444
           +F+ LGLRDFARIDGW+L  +S+  S  +   G T+SG +++TDINLISGMEQTSFLFQQ
Sbjct: 377 IFQKLGLRDFARIDGWYLAPNSNLSSPVSETLGGTKSGDIIFTDINLISGMEQTSFLFQQ 436

Query: 445 ASKVGFSHSNILRSIIYHACLRYPSLGSLDCMSGNFPRRSISSQRSQVYSSHESIRKVFV 504
           ASKVGFSHSNILR+I++ AC R+P    LD  +  +   S   Q S      E  +KVFV
Sbjct: 437 ASKVGFSHSNILRTIVHRACSRFP---HLDWYNYGY---SQLLQGSTTLEVSEDPQKVFV 496

Query: 505 IFGGDTSERQVSLMSGTNVWLNLQ 529
           IFGGDTSERQVS+MSGTNVW+NLQ
Sbjct: 497 IFGGDTSERQVSVMSGTNVWVNLQ 513