Spg032755 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg032755
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionMajor facilitator superfamily protein
Locationscaffold11: 5535934 .. 5537673 (+)
RNA-Seq ExpressionSpg032755
SyntenySpg032755
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCGAGAACAACCATGCAGTAGAAAATTGGCGGTTCGTGAAACAAGTCGTTCAAGGACGATGGTTTTCGGTATTCGCCTCGTTTCTGATCATGCTCGGCTGTGGTTCCACTTACTTATTCGGAACATACTCCAAATTGATGAAGACTCGATTCGATTACGATCAAACCCAAATCAACACGTTGGGATTTGCCAAAGATCTCGGCTCAAACCTTGGAGTTTTTGCCGGACTTCTGGCCGAGGTGGTTCCGTCGTGGGTCTTGTTCCTCATCGGCTCATCCGTTAACTTTCTTAGCTACTTCTTCATCTGGCTCTCTGTTACTGGCTACATCCAAAATCCCCGATTCTGGCTGATGTTCGTTTCCGTATGTATGTCTGCCAATGCACAGAACTTCCCCAACACTGCCATTATGGTCACCAACGTACGAAACTTCCCCGATCGACGAGGCATCATTTTGGGCCTTCTTAAGGGCTTTGTCGGCCTCGGTGGGGCCGTCCTAACCCAGGTATATTTCAGCATTTATGGCCATGCAAACCCAGCTGATCTCATCCTCCTTTTGTCCTGGCTTCCTTCTGTTGTGATTCTCCTCGTTTTTCTTTCGATTCGAACAATCAAAGCTCAAAAGCATCCACAGGAGCTCAAAGTGTTCTATCACTTGCTTTTTGTGTCCATAGCAATGGCAGTGTTCATCTTGTTTCTCACCTTAACACAAAAACATTCTGCCTTTTCTCACGCTGGCTATGTCGGGGGTGTTGTAGTCATCGTCGCCTTACTCTGTCTGCCTCTTCTCATTGCAATTAAAGAAGAATTGTTTCTCTTCAAACTCAACAAACAAACCGTGGATCCATCCGTCGCTATTGTAATTGCTGAAGACGAGAAAGCTACGGATGAGAAGATTTCAGAAGCTAATTCCCGGTCGTGGTTCTCAAATGTATTTAACAAACCGAAAAGAGGGGAGGATTTTAGCATCATGCAAGCTCTCTTCAGCTTAGACATGGCTCTTATTTTCGTAGCGGCAATTTCCGCTTGTGGTTCGTCTGTTGTCGCCATCGACAATCTTGGGCAGATCGCGGAATCACTTAAATACCCAGATCGTACTATAAGCATCTTCATTTCGTGGATTTCCATTTTCAATTTCTTCGGTCGGGTCTTCTCTGGTTTCATCTCGGAAACTCTTATGACCAAATACAAATTACCTCGTCCTCTCATGTTCGGCCTTACACATATCTTCATCTGCATTGGCCTATTCTCCATTGCCTTCCCATACACAAATTCAGTCTACACAGCTTCGTTGATCATTGGGTTTGGGTTTGGAGCCCAAGTTCCATTGCTGTTCACCTTAATCTCTGATCTCTTCGGGCTCAAACATTACTCGACGTTGCTCAACTGTGGGCAGATAGCAGTTCCATTGGGATCTTATATACTAAACGTGCATGTAGTGGGGAAGTTGTACGATCGGGAAGCCATTAAAGGCGGTAGTGTGAAGAATGGGAAGGGCCTAACGTGCACTGGAACCAATTGCTTTAGTGGGTCGTTCACAATTTTGGCCGCAGTGACTTTGTTTGGAGTGATGGCTTCGTTTGTGTTGGCTTACAGGACTCGGGACTTTTACAAAGGTGATACATACAAGAAATACAGAGATGATATGTGGATTACTCGACCAGATGTGGAATTGTATTCTTCCGATGATAAGAAGAACAGTGATCACAAAAAGAGCAATAATGATGGAAGCAAATAA

mRNA sequence

ATGGGCGAGAACAACCATGCAGTAGAAAATTGGCGGTTCGTGAAACAAGTCGTTCAAGGACGATGGTTTTCGGTATTCGCCTCGTTTCTGATCATGCTCGGCTGTGGTTCCACTTACTTATTCGGAACATACTCCAAATTGATGAAGACTCGATTCGATTACGATCAAACCCAAATCAACACGTTGGGATTTGCCAAAGATCTCGGCTCAAACCTTGGAGTTTTTGCCGGACTTCTGGCCGAGGTGGTTCCGTCGTGGGTCTTGTTCCTCATCGGCTCATCCGTTAACTTTCTTAGCTACTTCTTCATCTGGCTCTCTGTTACTGGCTACATCCAAAATCCCCGATTCTGGCTGATGTTCGTTTCCGTATGTATGTCTGCCAATGCACAGAACTTCCCCAACACTGCCATTATGGTCACCAACGTACGAAACTTCCCCGATCGACGAGGCATCATTTTGGGCCTTCTTAAGGGCTTTGTCGGCCTCGGTGGGGCCGTCCTAACCCAGGTATATTTCAGCATTTATGGCCATGCAAACCCAGCTGATCTCATCCTCCTTTTGTCCTGGCTTCCTTCTGTTGTGATTCTCCTCGTTTTTCTTTCGATTCGAACAATCAAAGCTCAAAAGCATCCACAGGAGCTCAAAGTGTTCTATCACTTGCTTTTTGTGTCCATAGCAATGGCAGTGTTCATCTTGTTTCTCACCTTAACACAAAAACATTCTGCCTTTTCTCACGCTGGCTATGTCGGGGGTGTTGTAGTCATCGTCGCCTTACTCTGTCTGCCTCTTCTCATTGCAATTAAAGAAGAATTGTTTCTCTTCAAACTCAACAAACAAACCGTGGATCCATCCGTCGCTATTGTAATTGCTGAAGACGAGAAAGCTACGGATGAGAAGATTTCAGAAGCTAATTCCCGGTCGTGGTTCTCAAATGTATTTAACAAACCGAAAAGAGGGGAGGATTTTAGCATCATGCAAGCTCTCTTCAGCTTAGACATGGCTCTTATTTTCGTAGCGGCAATTTCCGCTTGTGGTTCGTCTGTTGTCGCCATCGACAATCTTGGGCAGATCGCGGAATCACTTAAATACCCAGATCGTACTATAAGCATCTTCATTTCGTGGATTTCCATTTTCAATTTCTTCGGTCGGGTCTTCTCTGGTTTCATCTCGGAAACTCTTATGACCAAATACAAATTACCTCGTCCTCTCATGTTCGGCCTTACACATATCTTCATCTGCATTGGCCTATTCTCCATTGCCTTCCCATACACAAATTCAGTCTACACAGCTTCGTTGATCATTGGGTTTGGGTTTGGAGCCCAAGTTCCATTGCTGTTCACCTTAATCTCTGATCTCTTCGGGCTCAAACATTACTCGACGTTGCTCAACTGTGGGCAGATAGCAGTTCCATTGGGATCTTATATACTAAACGTGCATGTAGTGGGGAAGTTGTACGATCGGGAAGCCATTAAAGGCGGTAGTGTGAAGAATGGGAAGGGCCTAACGTGCACTGGAACCAATTGCTTTAGTGGGTCGTTCACAATTTTGGCCGCAGTGACTTTGTTTGGAGTGATGGCTTCGTTTGTGTTGGCTTACAGGACTCGGGACTTTTACAAAGGTGATACATACAAGAAATACAGAGATGATATGTGGATTACTCGACCAGATGTGGAATTGTATTCTTCCGATGATAAGAAGAACAGTGATCACAAAAAGAGCAATAATGATGGAAGCAAATAA

Coding sequence (CDS)

ATGGGCGAGAACAACCATGCAGTAGAAAATTGGCGGTTCGTGAAACAAGTCGTTCAAGGACGATGGTTTTCGGTATTCGCCTCGTTTCTGATCATGCTCGGCTGTGGTTCCACTTACTTATTCGGAACATACTCCAAATTGATGAAGACTCGATTCGATTACGATCAAACCCAAATCAACACGTTGGGATTTGCCAAAGATCTCGGCTCAAACCTTGGAGTTTTTGCCGGACTTCTGGCCGAGGTGGTTCCGTCGTGGGTCTTGTTCCTCATCGGCTCATCCGTTAACTTTCTTAGCTACTTCTTCATCTGGCTCTCTGTTACTGGCTACATCCAAAATCCCCGATTCTGGCTGATGTTCGTTTCCGTATGTATGTCTGCCAATGCACAGAACTTCCCCAACACTGCCATTATGGTCACCAACGTACGAAACTTCCCCGATCGACGAGGCATCATTTTGGGCCTTCTTAAGGGCTTTGTCGGCCTCGGTGGGGCCGTCCTAACCCAGGTATATTTCAGCATTTATGGCCATGCAAACCCAGCTGATCTCATCCTCCTTTTGTCCTGGCTTCCTTCTGTTGTGATTCTCCTCGTTTTTCTTTCGATTCGAACAATCAAAGCTCAAAAGCATCCACAGGAGCTCAAAGTGTTCTATCACTTGCTTTTTGTGTCCATAGCAATGGCAGTGTTCATCTTGTTTCTCACCTTAACACAAAAACATTCTGCCTTTTCTCACGCTGGCTATGTCGGGGGTGTTGTAGTCATCGTCGCCTTACTCTGTCTGCCTCTTCTCATTGCAATTAAAGAAGAATTGTTTCTCTTCAAACTCAACAAACAAACCGTGGATCCATCCGTCGCTATTGTAATTGCTGAAGACGAGAAAGCTACGGATGAGAAGATTTCAGAAGCTAATTCCCGGTCGTGGTTCTCAAATGTATTTAACAAACCGAAAAGAGGGGAGGATTTTAGCATCATGCAAGCTCTCTTCAGCTTAGACATGGCTCTTATTTTCGTAGCGGCAATTTCCGCTTGTGGTTCGTCTGTTGTCGCCATCGACAATCTTGGGCAGATCGCGGAATCACTTAAATACCCAGATCGTACTATAAGCATCTTCATTTCGTGGATTTCCATTTTCAATTTCTTCGGTCGGGTCTTCTCTGGTTTCATCTCGGAAACTCTTATGACCAAATACAAATTACCTCGTCCTCTCATGTTCGGCCTTACACATATCTTCATCTGCATTGGCCTATTCTCCATTGCCTTCCCATACACAAATTCAGTCTACACAGCTTCGTTGATCATTGGGTTTGGGTTTGGAGCCCAAGTTCCATTGCTGTTCACCTTAATCTCTGATCTCTTCGGGCTCAAACATTACTCGACGTTGCTCAACTGTGGGCAGATAGCAGTTCCATTGGGATCTTATATACTAAACGTGCATGTAGTGGGGAAGTTGTACGATCGGGAAGCCATTAAAGGCGGTAGTGTGAAGAATGGGAAGGGCCTAACGTGCACTGGAACCAATTGCTTTAGTGGGTCGTTCACAATTTTGGCCGCAGTGACTTTGTTTGGAGTGATGGCTTCGTTTGTGTTGGCTTACAGGACTCGGGACTTTTACAAAGGTGATACATACAAGAAATACAGAGATGATATGTGGATTACTCGACCAGATGTGGAATTGTATTCTTCCGATGATAAGAAGAACAGTGATCACAAAAAGAGCAATAATGATGGAAGCAAATAA

Protein sequence

MGENNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLGFAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSVCMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADLILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAFSHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEANSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKYPDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPYTNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGKLYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTYKKYRDDMWITRPDVELYSSDDKKNSDHKKSNNDGSK
Homology
BLAST of Spg032755 vs. NCBI nr
Match: XP_038895745.1 (uncharacterized protein LOC120083909 [Benincasa hispida])

HSP 1 Score: 849.4 bits (2193), Expect = 1.9e-242
Identity = 430/578 (74.39%), Postives = 494/578 (85.47%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           ++   ENWRFVKQVV+GRWFSVFA+FLIM+GCGSTYLFGTYSK++KT+FDY+QTQ++ LG
Sbjct: 3   SDRGAENWRFVKQVVEGRWFSVFAAFLIMIGCGSTYLFGTYSKVLKTKFDYNQTQLSHLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G ++NF SYF IWLSVT Y+  P  WLMF+ +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGLTLNFFSYFMIWLSVTEYVPKPELWLMFIYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +SANAQNF NTA+MVT+VRNFPD+RGIILGLLKGFVGLGGA+LTQ+YF+IYGH +P +L
Sbjct: 123 YISANAQNFANTAVMVTSVRNFPDQRGIILGLLKGFVGLGGAILTQIYFAIYGHQDPINL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLPS++  L FLS RTIKA+KHPQELKVF+HLL+VSI MAVFILFLT+TQKH+ F
Sbjct: 183 VLLLSWLPSIICFLFFLSFRTIKARKHPQELKVFFHLLYVSITMAVFILFLTITQKHTPF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           +HA YVGG  VIV LLCLPLLIAIKEELFLFKLNKQT DPSV + I   +    E+ISE 
Sbjct: 243 THANYVGGTSVIVVLLCLPLLIAIKEELFLFKLNKQTKDPSVVVSIPVQKL---EEISET 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           +S  + +   NKP+RGEDF I+QALFS DMALIF+A +SACGSSV AIDNLGQIAESL Y
Sbjct: 303 SSPCFSNKRSNKPQRGEDFGIVQALFSKDMALIFIATVSACGSSVAAIDNLGQIAESLNY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P  TI++F+SWISIFNFFGRV SGF+SETLMTKYKLPRPLMFGLT +   IGL SIAFPY
Sbjct: 363 PSETINVFVSWISIFNFFGRVCSGFVSETLMTKYKLPRPLMFGLTQVITSIGLISIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            +SVY ASLIIGFGFGAQ PLLF LISDLFGLKHYSTLLNCGQ+AVP GSYI+NVHVVGK
Sbjct: 423 KDSVYAASLIIGFGFGAQTPLLFALISDLFGLKHYSTLLNCGQLAVPFGSYIMNVHVVGK 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YDREA K G+VKNGKGLTCTGT+CFS SF IL  VTLFG M SFVLAYRTR+FYKGD Y
Sbjct: 483 FYDREATKIGNVKNGKGLTCTGTHCFSESFQILVLVTLFGAMTSFVLAYRTREFYKGDIY 542

Query: 544 KKYRDD-MWITRPDVELYSSDDKK-NSDHKKSNNDGSK 580
           K+YRDD MW T+ D ELYSSD+KK  +D   +++DGSK
Sbjct: 543 KRYRDDQMWSTQSDTELYSSDNKKMKNDTTDNDDDGSK 577

BLAST of Spg032755 vs. NCBI nr
Match: XP_022964773.1 (uncharacterized protein LOC111464774 [Cucurbita moschata])

HSP 1 Score: 834.7 bits (2155), Expect = 4.8e-238
Identity = 425/576 (73.78%), Postives = 491/576 (85.24%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           NN   E+W FVKQVV+GRWFSVF SFLIM+GCGS YLFGTYSKL+KT+FDY+QTQ+NTLG
Sbjct: 3   NNRGGESWLFVKQVVEGRWFSVFTSFLIMIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G S NF SYF IWLSV+ Y+  P+ WLMFV +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSNYVSKPQLWLMFVYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +++N+QNFPNTA+MVT+V NFPD+RGIILGLLKGFVGLGGA+LTQ+  +IYG+ N  DL
Sbjct: 123 YITSNSQNFPNTAVMVTSVGNFPDQRGIILGLLKGFVGLGGAILTQINLAIYGNQNSMDL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLPS+V  L FL IRTIKA+KHPQELKVFYHLL+VSIA+AVF+LFLT+TQ++  F
Sbjct: 183 LLLLSWLPSIVCSLCFLPIRTIKARKHPQELKVFYHLLYVSIAIAVFLLFLTITQRNIVF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           S AGY  GV VIV L+CLPLLIAIKEELFLFKL+KQT++PSV + I        E++ E 
Sbjct: 243 SQAGYASGVAVIVVLICLPLLIAIKEELFLFKLDKQTMNPSVLVSIPH------EQLEEI 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           +  S  SNV  KPKRGEDFSI+QAL S DMALIF+A +SACGSSV AIDNLGQIAESL+Y
Sbjct: 303 SLPS-VSNVCKKPKRGEDFSILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLEY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P+ +ISI +SWISIFNFFGRVFSGFISETLMTKYKLPRP MFGLT +F C GL SIAFPY
Sbjct: 363 PNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFMFGLTQLFTCTGLLSIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            NSVY ASLIIGFG GAQ PL+F +ISDLFGLKHYSTLLNCGQ+AVPLGSYI+NV V+G+
Sbjct: 423 INSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGR 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YD EA K G+VKNGKGLTCTGT+CFS SF ILAAVTLFG MASFVLAYRTRDFYKGD Y
Sbjct: 483 FYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGAMASFVLAYRTRDFYKGDVY 542

Query: 544 KKYRDDMWITRPDVELYSSDDKKNSDHKKSNNDGSK 580
           KKYRDD+ +T+ +VELYS+DD +N++HKK  +D SK
Sbjct: 543 KKYRDDIGVTQSNVELYSADD-RNNEHKKITDDRSK 570

BLAST of Spg032755 vs. NCBI nr
Match: XP_023521328.1 (uncharacterized protein LOC111785084 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833.9 bits (2153), Expect = 8.2e-238
Identity = 424/576 (73.61%), Postives = 491/576 (85.24%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           +NH  ++W FVKQVV+GRWFSVF SF IM+GCGS YLFGTYSKL+KT+FDY+QTQ+NTLG
Sbjct: 3   DNHETQSWLFVKQVVEGRWFSVFTSFPIMIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G S NF SYF IWLSV+ Y+  P  WLMFV +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSSYVPKPHLWLMFVYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +++N+QNFPNTA+MVT+V NFPD+RGIILGLLKGFVGLGGA+LTQ+  +I+G+ N  DL
Sbjct: 123 YITSNSQNFPNTAVMVTSVGNFPDQRGIILGLLKGFVGLGGAILTQINLAIHGNQNSIDL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLPS+V  L FL IRTIKA+KHPQELKVFYHLL+VSIA+AVF+LFLT+TQ++ AF
Sbjct: 183 LLLLSWLPSIVCFLCFLPIRTIKARKHPQELKVFYHLLYVSIAIAVFLLFLTITQRNIAF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           S AGY GGV VIV L+CLPLLIAIKEELFLFKL KQT++PSV + I  ++    ++IS+ 
Sbjct: 243 SQAGYAGGVAVIVVLICLPLLIAIKEELFLFKLGKQTMNPSVVVSIPREQL---KEISK- 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           NS    SNV  +P+RGEDFSI+QAL S DMALIF+A +SACGSSV AIDNLGQIAESLKY
Sbjct: 303 NSLPSVSNVCKEPQRGEDFSILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLKY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P+ +ISI +SWISIFNFFGRVFSGFISETLMTKYKLPRP +FGLT +F CIGL SIAFPY
Sbjct: 363 PNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFLFGLTQVFTCIGLLSIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            NSVY ASLIIGFG GAQ PL+F +ISDLFGLKHYSTLLNCGQ+AVPLGSYI+NV V+G+
Sbjct: 423 INSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGR 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YD EA K G+VKNGKGLTCTGT+CFS SF ILAAVTLFG MAS VLAYRTRDFYKGD Y
Sbjct: 483 FYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGAMASLVLAYRTRDFYKGDVY 542

Query: 544 KKYRDDMWITRPDVELYSSDDKKNSDHKKSNNDGSK 580
           KKYRDDM +   +VELYSSDD KN++HK   +D SK
Sbjct: 543 KKYRDDMEVKHSNVELYSSDD-KNNEHKMKTHDQSK 573

BLAST of Spg032755 vs. NCBI nr
Match: XP_022970244.1 (uncharacterized protein LOC111469253 [Cucurbita maxima])

HSP 1 Score: 833.6 bits (2152), Expect = 1.1e-237
Identity = 425/576 (73.78%), Postives = 493/576 (85.59%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           +N  VE+W FVKQVV+GRWFSVF SFLIM+GCGS YLFGTYSKL+KT+FDY+QTQ+NTLG
Sbjct: 3   DNRKVESWLFVKQVVEGRWFSVFTSFLIMIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G S NF SYF IWLSV+ Y+  P  WLMFV +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSNYVPKPHLWLMFVYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +++N+QNFPNTA+MVTNV NFP++RGIILGLLKGFVGLGGA+LTQ+  +IYG+ N  DL
Sbjct: 123 YITSNSQNFPNTAVMVTNVSNFPNQRGIILGLLKGFVGLGGAILTQINLAIYGNQNSIDL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLP++V  L FL IRTIKA+KHP+ELKVFYHLL+VSIA+AVF+LFLT+TQ++ AF
Sbjct: 183 LLLLSWLPTIVCFLCFLPIRTIKARKHPRELKVFYHLLYVSIAIAVFLLFLTITQRNIAF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           S AGY GG+VVIV L+CLPLLIAIKEELFLFKL KQT++PSV + +  ++    E+IS+ 
Sbjct: 243 SQAGYAGGIVVIVVLICLPLLIAIKEELFLFKLGKQTMNPSVVVSLPREQL---EEISKL 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           +  S  SNV  +P+RGEDF+I+QAL S DMALIF+A +SACGSSV AIDNLGQIAESLKY
Sbjct: 303 SLPS-VSNVCKEPQRGEDFNILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLKY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P+ +ISI +SWISIFNFFGRVFSGFISETLMTKYKLPRP MFGLT +F C GL SIAFPY
Sbjct: 363 PNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFMFGLTQLFTCTGLLSIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            NSVY ASLIIGFG GAQ PL+F +ISDLFGLKHYSTLLNCGQ+AVPLGSYI+NV V+G+
Sbjct: 423 INSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGR 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YD EA K G+VKNGKGLTCTGT+CFS SF ILAAVTLFG MASFVLAYRTRDFYKGD Y
Sbjct: 483 FYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGTMASFVLAYRTRDFYKGDVY 542

Query: 544 KKYRDDMWITRPDVELYSSDDKKNSDHKKSNNDGSK 580
           KKYRDDM  T+ +VEL S DD KNS+HKK  +D SK
Sbjct: 543 KKYRDDMEATQSNVELNSFDD-KNSEHKKKIDDESK 573

BLAST of Spg032755 vs. NCBI nr
Match: XP_023520298.1 (uncharacterized protein LOC111783614 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 833.6 bits (2152), Expect = 1.1e-237
Identity = 422/576 (73.26%), Postives = 488/576 (84.72%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           +NH  ++W FVKQVV+GRWFSVF SF IM+GCGS YLFGTYSKL+KT+FDY+QTQ+NTLG
Sbjct: 3   DNHETQSWLFVKQVVEGRWFSVFTSFPIMIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G S NF SYF IWLSV+ Y+  P  WLMFV +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSSYVPKPHLWLMFVYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +++N+QNFPNTA+MVT+V NFPD+RGIILGLLKGFVGLGGA+LTQ+  +I+G+ N  DL
Sbjct: 123 YITSNSQNFPNTAVMVTSVGNFPDQRGIILGLLKGFVGLGGAILTQINLAIHGNQNSIDL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLPS+V  L FL IRTIKA+KHPQELKVFYHLL+VSIA+AVF+LFLT+TQ++ AF
Sbjct: 183 LLLLSWLPSIVCFLCFLPIRTIKARKHPQELKVFYHLLYVSIAIAVFLLFLTITQRNIAF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           S AGY GGV VI+ L+CLPLLIAIKEELFLFKL KQT++PSV + I  ++     KIS  
Sbjct: 243 SQAGYAGGVAVIIVLICLPLLIAIKEELFLFKLGKQTMNPSVVVSIPREQLKEISKISLP 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           +     SNV  +P+RGEDFSI+QAL S DMALIF+A +SACGSSV AIDNLGQIAESLKY
Sbjct: 303 S----VSNVCKEPQRGEDFSILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLKY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P+ +ISI +SWISIFNFFGRVFSGFISETLMTKYKLPRP +FGLT +F CIGL SIAFPY
Sbjct: 363 PNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFLFGLTQVFTCIGLLSIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            NSVY ASLIIGFG GAQ PL+F +ISDLFGLKHYSTLLNCGQ+AVPLGSYI+NV V+G+
Sbjct: 423 INSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGR 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YD EA K G+VKNGKGLTCTGT+CFS SF ILAAVTLFG MAS VLAYRTRDFYKGD Y
Sbjct: 483 FYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGAMASLVLAYRTRDFYKGDVY 542

Query: 544 KKYRDDMWITRPDVELYSSDDKKNSDHKKSNNDGSK 580
           KKYRDDM +   +VELYSSDD KN++HK   +D SK
Sbjct: 543 KKYRDDMEVKHSNVELYSSDD-KNNEHKMKTHDQSK 573

BLAST of Spg032755 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 2.0e-29
Identity = 128/529 (24.20%), Postives = 246/529 (46.50%), Query Frame = 0

Query: 21  RWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLGFAKDLGSNLGVFAGLLA 80
           +W  + A+  I    G+ + F  YS  +K+     Q ++N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 81  EVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSVCMSANAQNFPNTAIMVT 140
              P  V+    +++ F+ Y   WL +T  I  P + L+F+   ++  +  + NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 141 NVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADLILLLSWLPSVVI----L 200
            +R+FP+ R + L L   F G+  A+ +  + +I  + + ++L LLL+ L  +V+    L
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAI--NPSSSNLYLLLNSLVPLVVSFAAL 222

Query: 201 LVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILF-LTLTQKHSAFSHAGYVGGVVVI 260
              L+  ++         +   H+  +   +AV   F L L+   ++ +   ++G VV++
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLL 282

Query: 261 VALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEANSRSW----FSN 320
           V  LC PLL+  + + FL  +N +    S   V+   ++  ++K S ++   +     + 
Sbjct: 283 VFPLCAPLLVYAR-DYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAK 342

Query: 321 VFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKYPDRTISIF 380
             N  + G++ S    +  L+  L ++A        +V  +NLGQIA+SL     T+   
Sbjct: 343 EGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNSTTL--- 402

Query: 381 ISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPYT--NSVYT 440
           ++  S F+FFGR+ S    + +  +++L R   F +  +   I  F +A   +   ++ T
Sbjct: 403 VTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQTALQT 462

Query: 441 ASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGKLYDREA 500
           A+ +IG   G       ++ SDLFG        N     +P+GS +L  ++   +Y+  A
Sbjct: 463 ATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIAASIYEANA 522

Query: 501 IKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFY 539
               +      + C G +C+  +F     +++ GV++S  L  RT+  Y
Sbjct: 523 SPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of Spg032755 vs. ExPASy TrEMBL
Match: A0A6J1HLW5 (uncharacterized protein LOC111464774 OS=Cucurbita moschata OX=3662 GN=LOC111464774 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.3e-238
Identity = 425/576 (73.78%), Postives = 491/576 (85.24%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           NN   E+W FVKQVV+GRWFSVF SFLIM+GCGS YLFGTYSKL+KT+FDY+QTQ+NTLG
Sbjct: 3   NNRGGESWLFVKQVVEGRWFSVFTSFLIMIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G S NF SYF IWLSV+ Y+  P+ WLMFV +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSNYVSKPQLWLMFVYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +++N+QNFPNTA+MVT+V NFPD+RGIILGLLKGFVGLGGA+LTQ+  +IYG+ N  DL
Sbjct: 123 YITSNSQNFPNTAVMVTSVGNFPDQRGIILGLLKGFVGLGGAILTQINLAIYGNQNSMDL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLPS+V  L FL IRTIKA+KHPQELKVFYHLL+VSIA+AVF+LFLT+TQ++  F
Sbjct: 183 LLLLSWLPSIVCSLCFLPIRTIKARKHPQELKVFYHLLYVSIAIAVFLLFLTITQRNIVF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           S AGY  GV VIV L+CLPLLIAIKEELFLFKL+KQT++PSV + I        E++ E 
Sbjct: 243 SQAGYASGVAVIVVLICLPLLIAIKEELFLFKLDKQTMNPSVLVSIPH------EQLEEI 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           +  S  SNV  KPKRGEDFSI+QAL S DMALIF+A +SACGSSV AIDNLGQIAESL+Y
Sbjct: 303 SLPS-VSNVCKKPKRGEDFSILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLEY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P+ +ISI +SWISIFNFFGRVFSGFISETLMTKYKLPRP MFGLT +F C GL SIAFPY
Sbjct: 363 PNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFMFGLTQLFTCTGLLSIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            NSVY ASLIIGFG GAQ PL+F +ISDLFGLKHYSTLLNCGQ+AVPLGSYI+NV V+G+
Sbjct: 423 INSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGR 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YD EA K G+VKNGKGLTCTGT+CFS SF ILAAVTLFG MASFVLAYRTRDFYKGD Y
Sbjct: 483 FYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGAMASFVLAYRTRDFYKGDVY 542

Query: 544 KKYRDDMWITRPDVELYSSDDKKNSDHKKSNNDGSK 580
           KKYRDD+ +T+ +VELYS+DD +N++HKK  +D SK
Sbjct: 543 KKYRDDIGVTQSNVELYSADD-RNNEHKKITDDRSK 570

BLAST of Spg032755 vs. ExPASy TrEMBL
Match: A0A6J1I4Z4 (uncharacterized protein LOC111469253 OS=Cucurbita maxima OX=3661 GN=LOC111469253 PE=4 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 5.2e-238
Identity = 425/576 (73.78%), Postives = 493/576 (85.59%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           +N  VE+W FVKQVV+GRWFSVF SFLIM+GCGS YLFGTYSKL+KT+FDY+QTQ+NTLG
Sbjct: 3   DNRKVESWLFVKQVVEGRWFSVFTSFLIMIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G S NF SYF IWLSV+ Y+  P  WLMFV +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSNYVPKPHLWLMFVYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +++N+QNFPNTA+MVTNV NFP++RGIILGLLKGFVGLGGA+LTQ+  +IYG+ N  DL
Sbjct: 123 YITSNSQNFPNTAVMVTNVSNFPNQRGIILGLLKGFVGLGGAILTQINLAIYGNQNSIDL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLP++V  L FL IRTIKA+KHP+ELKVFYHLL+VSIA+AVF+LFLT+TQ++ AF
Sbjct: 183 LLLLSWLPTIVCFLCFLPIRTIKARKHPRELKVFYHLLYVSIAIAVFLLFLTITQRNIAF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           S AGY GG+VVIV L+CLPLLIAIKEELFLFKL KQT++PSV + +  ++    E+IS+ 
Sbjct: 243 SQAGYAGGIVVIVVLICLPLLIAIKEELFLFKLGKQTMNPSVVVSLPREQL---EEISKL 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           +  S  SNV  +P+RGEDF+I+QAL S DMALIF+A +SACGSSV AIDNLGQIAESLKY
Sbjct: 303 SLPS-VSNVCKEPQRGEDFNILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLKY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P+ +ISI +SWISIFNFFGRVFSGFISETLMTKYKLPRP MFGLT +F C GL SIAFPY
Sbjct: 363 PNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFMFGLTQLFTCTGLLSIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            NSVY ASLIIGFG GAQ PL+F +ISDLFGLKHYSTLLNCGQ+AVPLGSYI+NV V+G+
Sbjct: 423 INSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGR 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YD EA K G+VKNGKGLTCTGT+CFS SF ILAAVTLFG MASFVLAYRTRDFYKGD Y
Sbjct: 483 FYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGTMASFVLAYRTRDFYKGDVY 542

Query: 544 KKYRDDMWITRPDVELYSSDDKKNSDHKKSNNDGSK 580
           KKYRDDM  T+ +VEL S DD KNS+HKK  +D SK
Sbjct: 543 KKYRDDMEATQSNVELNSFDD-KNSEHKKKIDDESK 573

BLAST of Spg032755 vs. ExPASy TrEMBL
Match: A0A6J1HLW0 (uncharacterized protein LOC111464768 OS=Cucurbita moschata OX=3662 GN=LOC111464768 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 1.2e-237
Identity = 424/576 (73.61%), Postives = 490/576 (85.07%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           NN   E+W FVKQVV+GRWFSVF SFLIM+GCGS YLFGTYSKL+KT+FDY+QTQ+NTLG
Sbjct: 3   NNRGGESWLFVKQVVEGRWFSVFTSFLIMIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G S NF SYF IWLSV+ Y+  P+ WLMFV +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSNYVSKPQLWLMFVYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +++N+QNFPNTA+MVT+V NFPD+RGIILGLLKGFVGLGGA+LTQ+  +IYG+ N  DL
Sbjct: 123 YITSNSQNFPNTAVMVTSVGNFPDQRGIILGLLKGFVGLGGAILTQINLAIYGNQNSMDL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLPS+V  L FL IRTIKA+KHPQELKVFYHLL+VSIA+AVF+LFLT+TQ++  F
Sbjct: 183 LLLLSWLPSIVCSLCFLPIRTIKARKHPQELKVFYHLLYVSIAIAVFLLFLTITQRNIVF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           S AGY  GV VIV L+CLPLLIAIKEELFLFKL+KQT++PSV + I        E++ E 
Sbjct: 243 SQAGYASGVAVIVLLICLPLLIAIKEELFLFKLDKQTMNPSVLVSIPH------EQLEEI 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           +  S  SNV  KPKRGEDFSI+QAL S DM LIF+A +SACGSSV AIDNLGQIAESL+Y
Sbjct: 303 SLPS-VSNVCKKPKRGEDFSILQALLSKDMTLIFIATVSACGSSVAAIDNLGQIAESLEY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P+ +ISI +SWISIFNFFGRVFSGFISETLMTKYKLPRP MFGLT +F C GL SIAFPY
Sbjct: 363 PNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFMFGLTQLFTCTGLLSIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            NSVY ASLIIGFG GAQ PL+F +ISDLFGLKHYSTLLNCGQ+AVPLGSYI+NV V+G+
Sbjct: 423 INSVYIASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGR 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YD EA K G+VKNGKGLTCTGT+CFS SF ILAAVTLFG MASFVLAYRTRDFYKGD Y
Sbjct: 483 FYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGAMASFVLAYRTRDFYKGDVY 542

Query: 544 KKYRDDMWITRPDVELYSSDDKKNSDHKKSNNDGSK 580
           KKYRDD+ +T+ +VELYS+DD +N++HKK  +D SK
Sbjct: 543 KKYRDDIGVTQSNVELYSADD-RNNEHKKITDDRSK 570

BLAST of Spg032755 vs. ExPASy TrEMBL
Match: A0A6J1HP67 (uncharacterized protein LOC111464769 OS=Cucurbita moschata OX=3662 GN=LOC111464769 PE=4 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 1.3e-236
Identity = 420/573 (73.30%), Postives = 484/573 (84.47%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           +NH  E+W FVKQVV+GRWFSVF SF IM+GCGS YLFGTYSKL+KT+FDY+QTQ+NTLG
Sbjct: 3   DNHETESWLFVKQVVEGRWFSVFTSFPIMIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G S NF SYF IWLSV+ Y+  P  WLMFV +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSSYVPKPHLWLMFVYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +++N+QNFPNTA+MVT+V NFPD+RGIILGLLKGFVGLGGA+LTQ+  +I+G+ N  DL
Sbjct: 123 YITSNSQNFPNTAVMVTSVGNFPDQRGIILGLLKGFVGLGGAILTQINLAIHGNQNSMDL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLPS+V  L FL IRTIKA+KHPQELKVFYHLL+VSIA+AVF+LFLT+TQ++ AF
Sbjct: 183 LLLLSWLPSIVCFLCFLPIRTIKARKHPQELKVFYHLLYVSIAIAVFLLFLTITQRNIAF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           S AGY GGV VI  L+CLPLLIAIKEELFLFKL KQT++PSV + I  ++     KIS  
Sbjct: 243 SQAGYAGGVAVIGVLICLPLLIAIKEELFLFKLGKQTMNPSVVVSIPREQLKEISKISLP 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           +      NV  +P+RGEDFSI+QAL S DMALIF+A +SACGSSV AIDNLGQIAESLKY
Sbjct: 303 S----VLNVCKEPQRGEDFSILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLKY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P+ +ISI +SWISIFNFFGRVFSGFISETLMTKYKLPRP MFGLT +F C GL SIAFPY
Sbjct: 363 PNHSISILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFMFGLTQVFTCTGLLSIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            NSVY ASLIIGFG GAQ PL+F +ISDLFGLKHYSTLLNCGQ+AVPLGSYI+NV V+G+
Sbjct: 423 INSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGR 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YD EA K G+VKNGKGLTCTGT+CFS SF ILAAVTLFG MASFVL+YRTRDFYKGD Y
Sbjct: 483 FYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGAMASFVLSYRTRDFYKGDVY 542

Query: 544 KKYRDDMWITRPDVELYSSDDKKNSDHKKSNND 577
           KKYRDDM +   +VELYSSDD KN++HK   +D
Sbjct: 543 KKYRDDMEVKHSNVELYSSDD-KNNEHKMKTDD 570

BLAST of Spg032755 vs. ExPASy TrEMBL
Match: A0A6J1HP71 (uncharacterized protein LOC111464779 OS=Cucurbita moschata OX=3662 GN=LOC111464779 PE=4 SV=1)

HSP 1 Score: 826.2 bits (2133), Expect = 8.3e-236
Identity = 424/576 (73.61%), Postives = 488/576 (84.72%), Query Frame = 0

Query: 4   NNHAVENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLG 63
           NN   E+W FVKQVV+GRWFSVF SFLIM+GCGS YLFGTYSKL+KT+FDY+QTQ+NTLG
Sbjct: 3   NNRGGESWLFVKQVVEGRWFSVFTSFLIMIGCGSPYLFGTYSKLLKTKFDYNQTQLNTLG 62

Query: 64  FAKDLGSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSV 123
           FAKDLGSNLGVFAGL AEV P W+LFL+G S NF SYF IWLSV+ Y+  P+ WLMFV +
Sbjct: 63  FAKDLGSNLGVFAGLFAEVAPPWMLFLVGISSNFFSYFMIWLSVSNYVSKPQLWLMFVYI 122

Query: 124 CMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADL 183
            +++N+QNFPNTA+MVT+V NFPD+RGIILGLLKGFVGLGGA+LTQ+  +IYG+ N  DL
Sbjct: 123 YITSNSQNFPNTAVMVTSVGNFPDQRGIILGLLKGFVGLGGAILTQINLAIYGNQNSMDL 182

Query: 184 ILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAF 243
           +LLLSWLPS+V  L FL IRTIKA+KHPQELKVFYHLL+VSIA+AVF+LFLT+TQ++  F
Sbjct: 183 LLLLSWLPSIVCSLCFLPIRTIKARKHPQELKVFYHLLYVSIAIAVFLLFLTITQRNIVF 242

Query: 244 SHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEA 303
           S AGY  GV VIV L+CLPLLIAIKEELFLFKL KQT++PSV + +  ++    E+IS+ 
Sbjct: 243 SQAGYASGVAVIVVLICLPLLIAIKEELFLFKLGKQTMNPSVVVSLPCEQL---EEISK- 302

Query: 304 NSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKY 363
           NS    SNV  KP+RGEDFSI+QAL S DMALIF+A +SACGSSV AIDNLGQIAESLKY
Sbjct: 303 NSLPSVSNVCKKPQRGEDFSILQALLSKDMALIFIATVSACGSSVAAIDNLGQIAESLKY 362

Query: 364 PDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPY 423
           P+ +I I +SWISIFNFFGRVFSGFISETLMTKYKLPRP MFGLT +F   GL SIAFPY
Sbjct: 363 PNHSIGILVSWISIFNFFGRVFSGFISETLMTKYKLPRPFMFGLTQLFTSTGLLSIAFPY 422

Query: 424 TNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGK 483
            NSVY ASLIIGFG GAQ PL+F +ISDLFGLKHYSTLLNCGQ+AVPLGSYI+NV V+G+
Sbjct: 423 INSVYVASLIIGFGLGAQTPLIFAIISDLFGLKHYSTLLNCGQLAVPLGSYIMNVEVIGR 482

Query: 484 LYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFYKGDTY 543
            YD EA K G+VKNGKGLTCTGT+CFS SF ILAAVTLFG MASFVLAYRTRDFYKGD Y
Sbjct: 483 FYDAEATKIGNVKNGKGLTCTGTHCFSESFMILAAVTLFGAMASFVLAYRTRDFYKGDVY 542

Query: 544 KKYRDDMWITRPDVELYSSDDKKNSDHKKSNNDGSK 580
           KKY+DDM  T+ +VEL S DD KN++HKK  +D SK
Sbjct: 543 KKYKDDMEATQSNVELNSFDD-KNNEHKKITDDRSK 573

BLAST of Spg032755 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 556.6 bits (1433), Expect = 2.3e-158
Identity = 288/553 (52.08%), Postives = 388/553 (70.16%), Query Frame = 0

Query: 9   ENWRFVKQVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLGFAKDL 68
           E  RF+     GRWF VFASFLIM   G+TYLFGTYSK +K+   YDQT +N LGF KDL
Sbjct: 5   ETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDL 64

Query: 69  GSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSVCMSAN 128
           G+N+GV +GL+AEV P+W +  IGS++NF+ YF IWL+VTG +  P+ W M + +C+ AN
Sbjct: 65  GANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGAN 124

Query: 129 AQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADLILLLS 188
           +QNF NT  +VT V+NFP+ RG++LGLLKG+VGL GA+ TQ+YF+IYGH +   LILL++
Sbjct: 125 SQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGH-DSKSLILLIA 184

Query: 189 WLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAFSHAGY 248
           WLP+ V L+    IR  K  +   EL VFY  L++SI +A+F++ + + +K   FS A Y
Sbjct: 185 WLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAY 244

Query: 249 VGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATD---EKISEAN- 308
                +  ALL +PL +++K+EL ++ + K  ++    + + + +K  D   +K ++ N 
Sbjct: 245 AASATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNG 304

Query: 309 ----SRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAES 368
               ++S FS VF+ P RGED++I+QAL S DM ++FVA     GSS+ A+DNLGQI ES
Sbjct: 305 EEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGES 364

Query: 369 LKYPDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIA 428
           L YP+ T+S F+S +SI+N+FGRVFSGF+SE L+ KYKLPRPLM  L  +  C G   IA
Sbjct: 365 LGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIA 424

Query: 429 FPYTNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHV 488
           FP   SVY AS+++GF FGAQ+PLLF +IS+LFGLK+YSTL NCGQ+A PLGSYILNV V
Sbjct: 425 FPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRV 484

Query: 489 VGKLYDREAIK-----GGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTR 548
            G LYD+EA+K     G + K+ K LTC G+ C+   F ILAAVT FG + S  LA RTR
Sbjct: 485 TGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTR 544

BLAST of Spg032755 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 496.9 bits (1278), Expect = 2.2e-140
Identity = 260/551 (47.19%), Postives = 367/551 (66.61%), Query Frame = 0

Query: 16  QVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLGFAKDLGSNLGVF 75
           Q++ GRWF  F S LIM   G+TY+FG YS  +K    YDQT +N L F KDLG+N+GV 
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 76  AGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSVCMSANAQNFPNT 135
           AGLL EV P W + LIG+ +NF  YF IWL+VT  I  P+ W M + +C+ AN+Q+F NT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 136 AIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADLILLLSWLPSVVI 195
             +VT V+NFP+ RG++LG+LKG+VGL GA++TQ+Y + YG  +  +LIL++ WLP++V 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGE-DTKELILMIGWLPAIVS 195

Query: 196 LLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAFSHAGYVGGVVVI 255
                +IR +K ++   ELKVFY+ L++S+ +A F++ + +  K S F+ + + G   V+
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 256 VALLCLPLLIAIKEELFLFKLNKQTV-DPSVAIVIAE------------DEKATDEKISE 315
           + LL LP+++ I EE  L+K  +  + DP+   V+ E            D + + E + +
Sbjct: 256 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEK 315

Query: 316 ANSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLK 375
             + S ++ VFN P+RG+D++I+QALFS+DM ++F+A I   G ++ AIDNLGQI  SL 
Sbjct: 316 VKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLG 375

Query: 376 YPDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFP 435
           YP R++S F+S +SI+N++GRV SG +SE  + KYK PRPLM  +  +  C G   IAF 
Sbjct: 376 YPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFN 435

Query: 436 YTNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVG 495
               +Y AS+IIGF FGAQ PLLF +IS++FGLK+YSTL N G +A P+GSY+LNV V G
Sbjct: 436 VPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAG 495

Query: 496 KLYDREAIK-----GGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDF 549
            LYD EA K     G +   G+ L C GT+CF  SF I+AAVTLFGV+ S VL  RT+ F
Sbjct: 496 YLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKF 555

BLAST of Spg032755 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 1.2e-74
Identity = 183/541 (33.83%), Postives = 303/541 (56.01%), Query Frame = 0

Query: 16  QVVQGRWFSVFASFLIMLGCGSTYLFGTYSKLMKTRFDYDQTQINTLGFAKDLGSNLGVF 75
           ++++ +W ++ AS  I    G++Y FG YS ++K+   YDQ+ ++T+   KD+G+N GVF
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 76  AGLLAEVVPS----------------WVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLM 135
           +GLL     S                WV+  +G+   F  YF IW SVTG I+ P   LM
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 136 FVSVCMSANAQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHAN 195
            + + ++A +Q F NTA +V+ V NF D  G  +G++KGF+GL GA+L Q+Y ++    +
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCA-GD 181

Query: 196 PADLILLLSWLPSVVILLVFLSIRTIKAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQK 255
           PA  ILLL+  P+V+ LLV   +R I       + K    L  VS+ +A +++ + + + 
Sbjct: 182 PASFILLLAVTPTVLSLLVMPLVR-IYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKN 241

Query: 256 HSAFSHAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATD-E 315
               S    +  +V ++ +L LPLLIA + +       ++TV    + +I+  +  T   
Sbjct: 242 TFGLSSWANIVTLVCLLVMLALPLLIARRAQR---DGMEKTVPHDYSPLISSPKATTSGN 301

Query: 316 KISEANSRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIA 375
           + SE +S+        +    E+ +++QA+  L   L+F+A I   GS +  I+N+ QI 
Sbjct: 302 QSSEGDSKV-------EAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIG 361

Query: 376 ESLKYPDRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFS 435
           ESL+Y    I+  +S  SI+NF GR  +G+ S+ L+ K   PRPL+   T   + IG   
Sbjct: 362 ESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLI 421

Query: 436 IAFPYTNSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNV 495
           IA  +  ++Y  S+I+G  +G+Q  L+ T+ S+LFG++H  T+ N   +A P+GSYI +V
Sbjct: 422 IASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSV 481

Query: 496 HVVGKLYDREAIKGGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFY 540
            ++G +YD+ A       +G+G TC G++CF  SF I+A+V  FG + + VL +RT+  Y
Sbjct: 482 RLIGYIYDKTA-------SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFRTKTLY 523

BLAST of Spg032755 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 1.2e-74
Identity = 176/535 (32.90%), Postives = 290/535 (54.21%), Query Frame = 0

Query: 11  WRF-VKQVVQGRWFSVFASFLIMLGCGSTYLF-GTYSKLMKTRFDYDQTQINTLGFAKDL 70
           WR  ++ +V  RW     +  +    G  YLF G+ S  +KT   Y+Q QI  LG AK+L
Sbjct: 5   WRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNL 64

Query: 71  GSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSVCMSAN 130
           G  +G  +G L+EV PSWV+ L+G++ N   Y  +WL VTG + N   W++FV++ +  N
Sbjct: 65  GDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTN 124

Query: 131 AQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADLILLLS 190
            + + NTA +V+ + NFP+ RG ++G+LKGF GL GA+LTQVY  ++  ++ + +IL+++
Sbjct: 125 GETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVA 184

Query: 191 WLPSVVILLVFLSIRTI----KAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAFS 250
             P VV+L +   +R +    +      +L+ F  +    + +AV++L L + Q     +
Sbjct: 185 LGPPVVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMT 244

Query: 251 HAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEAN 310
                    ++V  + +P+L+     +F+   N  +V P       +  +A    + E +
Sbjct: 245 QTIITTSGAILVIFMVVPVLVPF-SSVFISGNNVTSVKPEEGTSNVDQHEA--RTLIERS 304

Query: 311 SRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKYP 370
            R         P  GEDF+++QAL   D  LIF++ +   GS +  IDNLGQI  SL Y 
Sbjct: 305 DRP--PEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYS 364

Query: 371 DRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPYT 430
           +    IF+S ISI NF GRV  G+ SE ++ K  LPR L   +    + +GL   A  + 
Sbjct: 365 N--TKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWP 424

Query: 431 NSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGKL 490
             +Y  +++IG G+GA   +    +SD+FGLK + +L N    A+P+GS++ +  +   +
Sbjct: 425 GKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNI 484

Query: 491 YDREAIK-GGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFY 539
           YD  A K  G     + L CTG+ C+S + ++++ + L  ++ S  + YRTR FY
Sbjct: 485 YDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

BLAST of Spg032755 vs. TAIR 10
Match: AT5G50520.1 (Major facilitator superfamily protein )

HSP 1 Score: 278.5 bits (711), Expect = 1.2e-74
Identity = 176/535 (32.90%), Postives = 290/535 (54.21%), Query Frame = 0

Query: 11  WRF-VKQVVQGRWFSVFASFLIMLGCGSTYLF-GTYSKLMKTRFDYDQTQINTLGFAKDL 70
           WR  ++ +V  RW     +  +    G  YLF G+ S  +KT   Y+Q QI  LG AK+L
Sbjct: 5   WRHKLELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNL 64

Query: 71  GSNLGVFAGLLAEVVPSWVLFLIGSSVNFLSYFFIWLSVTGYIQNPRFWLMFVSVCMSAN 130
           G  +G  +G L+EV PSWV+ L+G++ N   Y  +WL VTG + N   W++FV++ +  N
Sbjct: 65  GDAIGFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTN 124

Query: 131 AQNFPNTAIMVTNVRNFPDRRGIILGLLKGFVGLGGAVLTQVYFSIYGHANPADLILLLS 190
            + + NTA +V+ + NFP+ RG ++G+LKGF GL GA+LTQVY  ++  ++ + +IL+++
Sbjct: 125 GETYYNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVA 184

Query: 191 WLPSVVILLVFLSIRTI----KAQKHPQELKVFYHLLFVSIAMAVFILFLTLTQKHSAFS 250
             P VV+L +   +R +    +      +L+ F  +    + +AV++L L + Q     +
Sbjct: 185 LGPPVVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMT 244

Query: 251 HAGYVGGVVVIVALLCLPLLIAIKEELFLFKLNKQTVDPSVAIVIAEDEKATDEKISEAN 310
                    ++V  + +P+L+     +F+   N  +V P       +  +A    + E +
Sbjct: 245 QTIITTSGAILVIFMVVPVLVPF-SSVFISGNNVTSVKPEEGTSNVDQHEA--RTLIERS 304

Query: 311 SRSWFSNVFNKPKRGEDFSIMQALFSLDMALIFVAAISACGSSVVAIDNLGQIAESLKYP 370
            R         P  GEDF+++QAL   D  LIF++ +   GS +  IDNLGQI  SL Y 
Sbjct: 305 DRP--PEKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSLGYS 364

Query: 371 DRTISIFISWISIFNFFGRVFSGFISETLMTKYKLPRPLMFGLTHIFICIGLFSIAFPYT 430
           +    IF+S ISI NF GRV  G+ SE ++ K  LPR L   +    + +GL   A  + 
Sbjct: 365 N--TKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAIDWP 424

Query: 431 NSVYTASLIIGFGFGAQVPLLFTLISDLFGLKHYSTLLNCGQIAVPLGSYILNVHVVGKL 490
             +Y  +++IG G+GA   +    +SD+FGLK + +L N    A+P+GS++ +  +   +
Sbjct: 425 GKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIASNI 484

Query: 491 YDREAIK-GGSVKNGKGLTCTGTNCFSGSFTILAAVTLFGVMASFVLAYRTRDFY 539
           YD  A K  G     + L CTG+ C+S + ++++ + L  ++ S  + YRTR FY
Sbjct: 485 YDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895745.11.9e-24274.39uncharacterized protein LOC120083909 [Benincasa hispida][more]
XP_022964773.14.8e-23873.78uncharacterized protein LOC111464774 [Cucurbita moschata][more]
XP_023521328.18.2e-23873.61uncharacterized protein LOC111785084 [Cucurbita pepo subsp. pepo][more]
XP_022970244.11.1e-23773.78uncharacterized protein LOC111469253 [Cucurbita maxima][more]
XP_023520298.11.1e-23773.26uncharacterized protein LOC111783614 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
F4I9E12.0e-2924.20Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HLW52.3e-23873.78uncharacterized protein LOC111464774 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1I4Z45.2e-23873.78uncharacterized protein LOC111469253 OS=Cucurbita maxima OX=3661 GN=LOC111469253... [more]
A0A6J1HLW01.2e-23773.61uncharacterized protein LOC111464768 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1HP671.3e-23673.30uncharacterized protein LOC111464769 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
A0A6J1HP718.3e-23673.61uncharacterized protein LOC111464779 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
Match NameE-valueIdentityDescription
AT2G28120.12.3e-15852.08Major facilitator superfamily protein [more]
AT2G39210.12.2e-14047.19Major facilitator superfamily protein [more]
AT1G74780.11.2e-7433.83Nodulin-like / Major Facilitator Superfamily protein [more]
AT5G50630.11.2e-7432.90Major facilitator superfamily protein [more]
AT5G50520.11.2e-7432.90Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 21..267
e-value: 1.5E-74
score: 250.7
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 15..215
e-value: 5.0E-6
score: 28.0
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 322..574
e-value: 2.1E-7
score: 32.1
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 22..538
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 560..579
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 4..549
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 4..549

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg032755.1Spg032755.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity