Spg032723 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg032723
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCarbonic anhydrase
Locationscaffold11: 8180803 .. 8183485 (-)
RNA-Seq ExpressionSpg032723
SyntenySpg032723
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGCTTTTGTGCTCTTTCTTCTTTTGCATTCTTTTGCTTTCATTCAAGGCAGCATCCCAAGAAGTTGGTGAGAGTCCCAAATTACGAACCGTGTTTTTTTTACGCTAAATATATATTTTAGTCTCTAACATCTGCATGTTACTTTCATTTAGACTCCATTGTCTAAAACATTTTAATTTAGGTCTCGTTTGATAACCATTTAGTTTTTTAAAATTAAACCTATAAACACTACTATCACCTATAAGTTTATTTGTTCTGTCATCTACTATCTTCTCATGTTTTCAAAAACTATGTCAAGTTTTAGAAACTAAAATGAGTAGTTTTGAAAAACTTGTGTTTTGTTTTTGGAATTTAACTAAGAATTCAAATGTATGCTTAACAAAGGTCATGAAAACCTTGGTAAAGAAATTAGGAAAAAACAAGCATAATTCTCAAAAACCACATCCGAAAACCAAATGGTTATCAAATGGGGTCTTAGTATCTCATGTTTCAATACTTTTCAATTATACTATTTCCGTTAATGAATGATAAAATCGATTGATGATAAATATGTATGAGATGGTACATAGAGTGAGTTTGACATAAATTTAGAACTCTTAACCTCTTTAAGTGACATGTTTATCTTCTAAATTTTTAACCTTAAAGCATATATTTAGCTTTGTTTTTCCTTGATAATTTCATCATGTTTTTTTTTTTTTTTATTGCTTTAAGGATTATGATTGTTTATATGCAGATGATGAAAGAGAGTTTAATTATGAAAAAGATGGAGAGAAAGGACCAACTCACTGGGGAGAGCTTAAACCAGAGTGGCATTCATGCAAGGCTGGCCTGATGCAATCTCCGATCGATATGTTGCACGAAAGAGTAGAAATTATTTCGCATTTGGGAGACCTCAAAATCAACTACAAACCTTCTAATGCCACTCTCAAGAATAGAGGCCATGATATGATGGTAAATAAATATTATCATGTGAATAGAAATTATGGTTTTTTAAGCTCGAAACTCCCATTTTTTTCCCTTCACCTCATAAATCAAATTAATATTTATGCTCAAGTGTGGTGAGGAAAGTGTTGAAAAATATATCAAATAATTAAATTCAATTTTATTTGAGCTTATATTAACTTTAATGATAATTTAATACGATAGGTTTTTTTTTTTTACAACATGTGGGATGGGGGATTTGAACATCTGACCTCTTAGAATCGAGAGTACAAATTTTATGCTAGTTGAGTTATGCTCTTGTTGCTTAATACGATATGTTTATGAGATCAAACGCTTTAATATGTCAAGTATTTGTCCTTTTTGATTCATAATGATAATGTTTTGGGCCATATGAAATAGAATGAATATCTATGTGCTACACATAAAAGGAAAGTATTGTGGTTGATGCAGATGAAATGGGTAGATGGAGGTGGATACATGGAAATGAATGGAACTCAATATAAACTAAAACAGTGTCATTGGCACTCACCTTCCGAGCACACCATTGATGGCAAGAATTTTGATCTTGAAGCTCACTTGGTTCATGAAAGCTCAAATGGCATGATTTCTGTAATTGGAATCTTGTATCAGATTGGAGAACCTGATTATTTCTTATCAACAGTGAGTTTAATTCATAATCTCCAGAAATTTAATATTATTGTGCAATTGTAATTCACTTCATTTTCAATCTTGTTCTATCAGATTAAGGATCATTTAGAAGTTGTCTCTGATACCTATGAAGAAGAGAGGGTGATTGGAATAATTGATCCAACTTTGCTGAAGATAGGCAGCAAACAATATTATAGATACATGGGCTCCCTTACTGTTCCACCGTGCACTGAAAATGTAACGTGGACCATGATCCATGAGGTACTTTTTTTAGTAGTTAATATTTTATATAGAGTAGAGGTAGGCTTAAAATTTGAATCTTTTGCAATATCATTTTTTTTCCTCTATTGCATATTCATAGTTCATTTCTCACAAATATAATCTCTTTATTTAGTTAGAAACCTTAAACTTATGGTATGTATGATGCATAAATGTAATTGGAAATATTTATTTGCTTAATTTGATTTTTTGATTTCACCAGGTGAGGACTATTACAGAAGAACAAGTGAAATTACTTCGAGTTGCTGTCCATGATGTAAGTTCGAATAATATTAATTGGCATACTAGAATATACACTTTTAATAAAAAAAAAATTATAGTAATATAATTTTTTAAAAAACATACTATAGATATGTTATGAAAATACCCTCACATTCAACTCAATTATATTTATGTAGTGTGTATCAAAAAAAAAAAAAAAAAAAAATTATATTTATGTAGGTTTGATAACCATATTATTTTTAAATCTTTGATTTTTTTTTTTTTTTTTAATCTCTTAGTCCTTTTTTTTCAGTTGCTTCAAACTAAATTTTATAGTTTTAAAATTTAACTACAATTTTAAGAGCGTTTTTGAGAGGTAAGATTACAAAAATAAGGAAATTTTGAGTAAATAAACATAGTTTTTGAAAGAAAAAAAAAAAACTTAAACAATTAGATCAAAATTAAAGATGAATTTTTCATTTTATATATCTGTTAAAAATAAAAGTTTGATATGTGAATGCAGGACTCGGATACTAATGCAAGACCATTACAACCCATCTATGATCGGACGATGAAACTCCAAAGACCGAAAGAAAGTGAGGATTGA

mRNA sequence

ATGGGGAAGCTTTTGTGCTCTTTCTTCTTTTGCATTCTTTTGCTTTCATTCAAGGCAGCATCCCAAGAAGTTGATGATGAAAGAGAGTTTAATTATGAAAAAGATGGAGAGAAAGGACCAACTCACTGGGGAGAGCTTAAACCAGAGTGGCATTCATGCAAGGCTGGCCTGATGCAATCTCCGATCGATATGTTGCACGAAAGAGTAGAAATTATTTCGCATTTGGGAGACCTCAAAATCAACTACAAACCTTCTAATGCCACTCTCAAGAATAGAGGCCATGATATGATGATGAAATGGGTAGATGGAGGTGGATACATGGAAATGAATGGAACTCAATATAAACTAAAACAGTGTCATTGGCACTCACCTTCCGAGCACACCATTGATGGCAAGAATTTTGATCTTGAAGCTCACTTGGTTCATGAAAGCTCAAATGGCATGATTTCTGTAATTGGAATCTTGTATCAGATTGGAGAACCTGATTATTTCTTATCAACAATTAAGGATCATTTAGAAGTTGTCTCTGATACCTATGAAGAAGAGAGGGTGATTGGAATAATTGATCCAACTTTGCTGAAGATAGGCAGCAAACAATATTATAGATACATGGGCTCCCTTACTGTTCCACCGTGCACTGAAAATGTAACGTGGACCATGATCCATGAGGTGAGGACTATTACAGAAGAACAAGTGAAATTACTTCGAGTTGCTGTCCATGATGACTCGGATACTAATGCAAGACCATTACAACCCATCTATGATCGGACGATGAAACTCCAAAGACCGAAAGAAAGTGAGGATTGA

Coding sequence (CDS)

ATGGGGAAGCTTTTGTGCTCTTTCTTCTTTTGCATTCTTTTGCTTTCATTCAAGGCAGCATCCCAAGAAGTTGATGATGAAAGAGAGTTTAATTATGAAAAAGATGGAGAGAAAGGACCAACTCACTGGGGAGAGCTTAAACCAGAGTGGCATTCATGCAAGGCTGGCCTGATGCAATCTCCGATCGATATGTTGCACGAAAGAGTAGAAATTATTTCGCATTTGGGAGACCTCAAAATCAACTACAAACCTTCTAATGCCACTCTCAAGAATAGAGGCCATGATATGATGATGAAATGGGTAGATGGAGGTGGATACATGGAAATGAATGGAACTCAATATAAACTAAAACAGTGTCATTGGCACTCACCTTCCGAGCACACCATTGATGGCAAGAATTTTGATCTTGAAGCTCACTTGGTTCATGAAAGCTCAAATGGCATGATTTCTGTAATTGGAATCTTGTATCAGATTGGAGAACCTGATTATTTCTTATCAACAATTAAGGATCATTTAGAAGTTGTCTCTGATACCTATGAAGAAGAGAGGGTGATTGGAATAATTGATCCAACTTTGCTGAAGATAGGCAGCAAACAATATTATAGATACATGGGCTCCCTTACTGTTCCACCGTGCACTGAAAATGTAACGTGGACCATGATCCATGAGGTGAGGACTATTACAGAAGAACAAGTGAAATTACTTCGAGTTGCTGTCCATGATGACTCGGATACTAATGCAAGACCATTACAACCCATCTATGATCGGACGATGAAACTCCAAAGACCGAAAGAAAGTGAGGATTGA

Protein sequence

MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQSPIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYEEERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVHDDSDTNARPLQPIYDRTMKLQRPKESED
Homology
BLAST of Spg032723 vs. NCBI nr
Match: XP_022152913.1 (alpha carbonic anhydrase 7-like [Momordica charantia])

HSP 1 Score: 510.8 bits (1314), Expect = 7.3e-141
Identity = 239/268 (89.18%), Postives = 257/268 (95.90%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           MGKLLCSFFFCILLLSFKAASQEVDDEREF+YE+DGEKGP+HWGELK EWH CKAG MQS
Sbjct: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFDYERDGEKGPSHWGELKAEWHKCKAGSMQS 60

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120
           PID+ H+RVE+ISHLGDLK NY PSNAT+KNRGHD+MMKWVDGGGYMEMNGTQYKLKQCH
Sbjct: 61  PIDLYHQRVEVISHLGDLKTNYIPSNATIKNRGHDIMMKWVDGGGYMEMNGTQYKLKQCH 120

Query: 121 WHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYE 180
           WHSPSEHTIDGKNFDLEAHLVHESS G+IS IGILYQIGEPDYFLST+KDHLEV+SDT+E
Sbjct: 121 WHSPSEHTIDGKNFDLEAHLVHESSTGIISAIGILYQIGEPDYFLSTMKDHLEVISDTHE 180

Query: 181 EERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVH 240
           EER IGIIDPTLLKIGSKQYYRY+GSLTVPPCTENV WTMI+EVRT+TEEQVK LRVAVH
Sbjct: 181 EERAIGIIDPTLLKIGSKQYYRYIGSLTVPPCTENVIWTMIYEVRTVTEEQVKSLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRTMKLQRPKESED 269
           DDSDTNARPLQPIY+RTM+LQRPKESE+
Sbjct: 241 DDSDTNARPLQPIYNRTMQLQRPKESEN 268

BLAST of Spg032723 vs. NCBI nr
Match: KAG6583572.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 510.4 bits (1313), Expect = 9.6e-141
Identity = 238/267 (89.14%), Postives = 257/267 (96.25%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           M KLLCSFFFCILLLSFKA SQEV+DER F+YE DG KGPTHWGEL+PEWH+CKAGLMQS
Sbjct: 1   MEKLLCSFFFCILLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120
           PIDMLHERVE+ISHLGDLKINYKPSNATLKNRGHDMMMKWV+GGG+ME+NG QYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWVEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYE 180
           WHSPSEHTIDGK FDLEAHLVHESSNGM+SVIGILYQIGEPDYFLSTIKDHLEVVSD++E
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180

Query: 181 EERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVH 240
           EER+IG+IDPTL+KIGSK+YYRYMGSLTVPPCTE+V WTMIHEVRT+TEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRTMKLQRPKESE 268
           DDSDTNARPLQPIYDR  +LQ+PKESE
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQKPKESE 267

BLAST of Spg032723 vs. NCBI nr
Match: XP_022970331.1 (alpha carbonic anhydrase 7-like [Cucurbita maxima])

HSP 1 Score: 509.6 bits (1311), Expect = 1.6e-140
Identity = 237/267 (88.76%), Postives = 256/267 (95.88%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           M KLLCSFFFC LLLSFKA SQEV+DER F+YE DG KGPTHWGEL+PEWH+CKAGLMQS
Sbjct: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120
           PIDMLHERVE+ISHLGDLKINYKPSNATLKNRGHDMMMKW++GGG+ME+NG QYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYE 180
           WHSPSEHTIDGK FDLEAHLVHESSNGM+SVIGILYQIGEPDYFLSTIKDHLEVVSD++E
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180

Query: 181 EERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVH 240
           EER+IG+IDPTL+KIGSK+YYRYMGSLTVPPCTE+V WTMIHEVRT+TEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRTMKLQRPKESE 268
           DDSDTNARPLQPIYDR  +LQRPKESE
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQRPKESE 267

BLAST of Spg032723 vs. NCBI nr
Match: XP_023520292.1 (alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508.1 bits (1307), Expect = 4.8e-140
Identity = 237/267 (88.76%), Postives = 256/267 (95.88%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           M KLLCSFFFCILLLSFKA SQEV+DER F+YE DG KGPTHWGEL+PEWH+CKAGLMQS
Sbjct: 1   MEKLLCSFFFCILLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120
           PIDMLHERVE+ISHLGDLKINYKPSNATLKNRGHDMMMKWV+GGG+ME+NG QYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWVEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYE 180
           WHSPSEHTIDGK FDLEAHLVHESSNGM+SVIGILYQIGEPDYFLSTIKDHLE+VSD++E
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLELVSDSHE 180

Query: 181 EERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVH 240
           EER+IG+IDPT +KIGSK+YYRYMGSLTVPPCTE+V WTMIHEVRT+TEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTRIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRTMKLQRPKESE 268
           DDSDTNARPLQPIYDR  +LQRPKESE
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQRPKESE 267

BLAST of Spg032723 vs. NCBI nr
Match: XP_022964924.1 (alpha carbonic anhydrase 7-like [Cucurbita moschata])

HSP 1 Score: 507.7 bits (1306), Expect = 6.2e-140
Identity = 237/267 (88.76%), Postives = 256/267 (95.88%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           M KLLCSFFFCILLLSFKA SQEV+DER F+YE DG KGPTHWGEL+PEWH+CKAGLMQS
Sbjct: 1   MEKLLCSFFFCILLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120
           PIDMLHERVE+ISHLGDLKINYKPSNATLKNRGHDMMMKWV+GGG+ME+NG QYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWVEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYE 180
           WHSPSEHTIDGK FDLEAHLVHESSNGM+SVIGILYQIGEPDYFLSTIKDHLEVVS ++E
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSGSHE 180

Query: 181 EERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVH 240
           EER+IG+IDPTL+KIGSK+YYRYMGSLTVPPCTE+V WTMIHEVRT+TEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRTMKLQRPKESE 268
           DDSDTNARPLQPIYDR  +LQ+PKESE
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQKPKESE 267

BLAST of Spg032723 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 1.7e-87
Identity = 147/259 (56.76%), Postives = 201/259 (77.61%), Query Frame = 0

Query: 5   LCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQSPIDM 64
           L S F  + + S  ++  EV+DEREFNY+K+ EKGP  WGELKPEW  C  G MQSPID+
Sbjct: 15  LFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDL 74

Query: 65  LHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCHWHSP 124
           ++ERV I+SHLG L  +Y PSNATLKNRGHD+M+K+ DG G +++NG +Y+L+Q HWHSP
Sbjct: 75  MNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWHSP 134

Query: 125 SEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYEEERV 184
           SEHTI+G+ F LE H+VHE  N  ++V+ +LY+IG  D F+ +++  LE +++  E E+ 
Sbjct: 135 SEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKN 194

Query: 185 IGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVHDDSD 244
           +G+IDPT +KIGS++YYRY GSLT PPCT+NVTW+++ +VRT+T +QVKLLRVAVHDD++
Sbjct: 195 VGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDAN 254

Query: 245 TNARPLQPIYDRTMKLQRP 264
           +NARP+QP   R + L RP
Sbjct: 255 SNARPVQPTNKRIVHLYRP 273

BLAST of Spg032723 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 7.3e-75
Identity = 140/259 (54.05%), Postives = 189/259 (72.97%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLS--FKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLM 60
           M  +    F   L LS  F A S EVDDE EF+Y++  E GP +WG ++P+W  C +G +
Sbjct: 3   MAAITKMLFISFLFLSSVFLARSGEVDDESEFSYDEKSENGPANWGNIRPDWKEC-SGKL 62

Query: 61  QSPIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQ 120
           QSPID+   R E++S+L  L+ +YKPSNATL NRGHD+M++ +D GGY+++N TQY+LKQ
Sbjct: 63  QSPIDIFDLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLR-LDDGGYLKINETQYQLKQ 122

Query: 121 CHWHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIG-EPDYFLSTIKDHLEVVSD 180
            HWH+PSEHTI+G+ F+LEAHLVHES+NG   VIGI+Y+IG  PD FLS I++ L+V ++
Sbjct: 123 LHWHTPSEHTINGERFNLEAHLVHESNNGKFVVIGIVYEIGLWPDPFLSMIENDLKVPAN 182

Query: 181 TYEEERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRV 240
               ER IGIIDP  +K+  K+Y+RY+GSLT PPCTE V W +  +V+T+T  Q+KLL+ 
Sbjct: 183 KKGIERGIGIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQE 242

Query: 241 AVHDDSDTNARPLQPIYDR 257
           AVHD  +TNARP QP  +R
Sbjct: 243 AVHDGFETNARPTQPENER 259

BLAST of Spg032723 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 2.1e-74
Identity = 134/265 (50.57%), Postives = 186/265 (70.19%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           +G +    F  I ++S      EV+DE +FNYEK GEKGP +WG LKPEW  C  G MQS
Sbjct: 6   IGYVFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQS 65

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDG--GGYMEMNGTQYKLKQ 120
           PID+  +RV I  +LG L+  Y PSNAT+KNRGHD+MMK+  G  G  + +NGT+YKL+Q
Sbjct: 66  PIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQ 125

Query: 121 CHWHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDT 180
            HWHSPSEHT++GK F LE H+VH+S +G  +V+   Y++G+PDYFL T++ +L+ ++DT
Sbjct: 126 IHWHSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDT 185

Query: 181 YEEERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVA 240
           +E +  + ++ P      SK YYR++GSLT PPC+ENV WT+  E+RT+T +Q+ +LRV 
Sbjct: 186 HESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVT 245

Query: 241 VHDDSDTNARPLQPIYDRTMKLQRP 264
           VHD S++NARPLQ   +R + L  P
Sbjct: 246 VHDQSNSNARPLQRKNERPVALYIP 270

BLAST of Spg032723 vs. ExPASy Swiss-Prot
Match: Q9FM99 (Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3)

HSP 1 Score: 270.0 bits (689), Expect = 2.9e-71
Identity = 130/248 (52.42%), Postives = 170/248 (68.55%), Query Frame = 0

Query: 19  AASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQSPIDMLHERVEIISHLGDL 78
           A   EV+DE EF+YE  G KGP  WG L  EW  C  G MQSPID+  + V + +  G L
Sbjct: 129 APGGEVEDETEFSYETKGNKGPAKWGTLDAEWKMCGIGKMQSPIDLRDKNVVVSNKFGLL 188

Query: 79  KINYKPSNATLKNRGHDMMMKWVDG--GGYMEMNGTQYKLKQCHWHSPSEHTIDGKNFDL 138
           +  Y PSN T+KNRGHD+M+K+  G  G  + + GT+Y+L+Q HWHSPSEHTI+GK F L
Sbjct: 189 RSQYLPSNTTIKNRGHDIMLKFKGGNKGIGVTIRGTRYQLQQLHWHSPSEHTINGKRFAL 248

Query: 139 EAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYEEERVIGIIDPTLLKIG 198
           E HLVHES +   +V+  LY +G  D FL +++  L+ ++DT+  E  +GIIDP  L   
Sbjct: 249 EEHLVHESKDKRYAVVAFLYNLGASDPFLFSLEKQLKKITDTHASEEHVGIIDPKKLSFE 308

Query: 199 SKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVHDDSDTNARPLQPIYDR 258
           SK YYRY GSLT PPC+ENV W++  E+RT++ +QVKLLRVAVHD SD+NARPLQ +  R
Sbjct: 309 SKHYYRYSGSLTAPPCSENVIWSVSKEIRTVSSKQVKLLRVAVHDASDSNARPLQAVNKR 368

Query: 259 TMKLQRPK 265
            + L +PK
Sbjct: 369 KVYLYKPK 376

BLAST of Spg032723 vs. ExPASy Swiss-Prot
Match: F4IHR4 (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2)

HSP 1 Score: 257.7 bits (657), Expect = 1.5e-67
Identity = 116/224 (51.79%), Postives = 165/224 (73.66%), Query Frame = 0

Query: 4   LLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQSPID 63
           +L SF   +  LS     +EV+DE EF+YE + E GP  WG+L+PEW  C  G MQSPID
Sbjct: 13  VLTSFVTTVSCLSAATDYREVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPID 72

Query: 64  MLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKW-VDGGGYMEMNGTQYKLKQCHWH 123
           ++++RV +++HL  L  +YKP NATLKNRGHDMM+K+  +G G + +NGT+YKL Q HWH
Sbjct: 73  LMNKRVRLVTHLKKLTRHYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWH 132

Query: 124 SPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYEEE 183
           SPSEHT++G+ F LE H+VHE+ NG ++V+ +LY+IG PD FL  +++ L  ++D  E E
Sbjct: 133 SPSEHTMNGRRFALELHMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAE 192

Query: 184 RVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRT 227
           + + +IDP  +KIGS+++YRY+GSLT PPCT+NV WT++ +V T
Sbjct: 193 KYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 236

BLAST of Spg032723 vs. ExPASy TrEMBL
Match: A0A6J1DG52 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020526 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 3.6e-141
Identity = 239/268 (89.18%), Postives = 257/268 (95.90%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           MGKLLCSFFFCILLLSFKAASQEVDDEREF+YE+DGEKGP+HWGELK EWH CKAG MQS
Sbjct: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFDYERDGEKGPSHWGELKAEWHKCKAGSMQS 60

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120
           PID+ H+RVE+ISHLGDLK NY PSNAT+KNRGHD+MMKWVDGGGYMEMNGTQYKLKQCH
Sbjct: 61  PIDLYHQRVEVISHLGDLKTNYIPSNATIKNRGHDIMMKWVDGGGYMEMNGTQYKLKQCH 120

Query: 121 WHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYE 180
           WHSPSEHTIDGKNFDLEAHLVHESS G+IS IGILYQIGEPDYFLST+KDHLEV+SDT+E
Sbjct: 121 WHSPSEHTIDGKNFDLEAHLVHESSTGIISAIGILYQIGEPDYFLSTMKDHLEVISDTHE 180

Query: 181 EERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVH 240
           EER IGIIDPTLLKIGSKQYYRY+GSLTVPPCTENV WTMI+EVRT+TEEQVK LRVAVH
Sbjct: 181 EERAIGIIDPTLLKIGSKQYYRYIGSLTVPPCTENVIWTMIYEVRTVTEEQVKSLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRTMKLQRPKESED 269
           DDSDTNARPLQPIY+RTM+LQRPKESE+
Sbjct: 241 DDSDTNARPLQPIYNRTMQLQRPKESEN 268

BLAST of Spg032723 vs. ExPASy TrEMBL
Match: A0A6J1I0A8 (Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469326 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 7.9e-141
Identity = 237/267 (88.76%), Postives = 256/267 (95.88%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           M KLLCSFFFC LLLSFKA SQEV+DER F+YE DG KGPTHWGEL+PEWH+CKAGLMQS
Sbjct: 1   MEKLLCSFFFCFLLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120
           PIDMLHERVE+ISHLGDLKINYKPSNATLKNRGHDMMMKW++GGG+ME+NG QYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWIEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYE 180
           WHSPSEHTIDGK FDLEAHLVHESSNGM+SVIGILYQIGEPDYFLSTIKDHLEVVSD++E
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSDSHE 180

Query: 181 EERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVH 240
           EER+IG+IDPTL+KIGSK+YYRYMGSLTVPPCTE+V WTMIHEVRT+TEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRTMKLQRPKESE 268
           DDSDTNARPLQPIYDR  +LQRPKESE
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQRPKESE 267

BLAST of Spg032723 vs. ExPASy TrEMBL
Match: A0A6J1HPL0 (Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464882 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 3.0e-140
Identity = 237/267 (88.76%), Postives = 256/267 (95.88%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           M KLLCSFFFCILLLSFKA SQEV+DER F+YE DG KGPTHWGEL+PEWH+CKAGLMQS
Sbjct: 1   MEKLLCSFFFCILLLSFKAQSQEVEDERAFDYESDGIKGPTHWGELRPEWHACKAGLMQS 60

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120
           PIDMLHERVE+ISHLGDLKINYKPSNATLKNRGHDMMMKWV+GGG+ME+NG QYKLKQCH
Sbjct: 61  PIDMLHERVEVISHLGDLKINYKPSNATLKNRGHDMMMKWVEGGGFMEVNGMQYKLKQCH 120

Query: 121 WHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYE 180
           WHSPSEHTIDGK FDLEAHLVHESSNGM+SVIGILYQIGEPDYFLSTIKDHLEVVS ++E
Sbjct: 121 WHSPSEHTIDGKKFDLEAHLVHESSNGMVSVIGILYQIGEPDYFLSTIKDHLEVVSGSHE 180

Query: 181 EERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVH 240
           EER+IG+IDPTL+KIGSK+YYRYMGSLTVPPCTE+V WTMIHEVRT+TEEQVKLLRVAVH
Sbjct: 181 EERIIGVIDPTLIKIGSKRYYRYMGSLTVPPCTESVMWTMIHEVRTVTEEQVKLLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRTMKLQRPKESE 268
           DDSDTNARPLQPIYDR  +LQ+PKESE
Sbjct: 241 DDSDTNARPLQPIYDRKTQLQKPKESE 267

BLAST of Spg032723 vs. ExPASy TrEMBL
Match: A0A6J1DHJ2 (Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020525 PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 2.4e-137
Identity = 231/268 (86.19%), Postives = 254/268 (94.78%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           MGK LCSFFFCILLLSFKAASQEV++EREF+YE DGE+GP+HWG+L+PEW  CKAG MQS
Sbjct: 1   MGKFLCSFFFCILLLSFKAASQEVENEREFDYEDDGERGPSHWGDLRPEWQKCKAGFMQS 60

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCH 120
           PID+LH+RVE+ISHLGDL+ NY PSNAT+KNRGHD+MMKWVDGGGYMEMNG QYKLKQCH
Sbjct: 61  PIDLLHQRVEVISHLGDLETNYIPSNATIKNRGHDIMMKWVDGGGYMEMNGXQYKLKQCH 120

Query: 121 WHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYE 180
           WHSPSEHTIDGKNFDLEAHLVHESS G+ISVIGILYQIGEPDYFLST+KDHLEV+SDT+E
Sbjct: 121 WHSPSEHTIDGKNFDLEAHLVHESSTGIISVIGILYQIGEPDYFLSTMKDHLEVISDTHE 180

Query: 181 EERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVH 240
           EER+IGIIDPTLLKIGSKQYYRY+GSLTVPPCTENV WTMI EVRT+TEEQVK LRVAVH
Sbjct: 181 EERIIGIIDPTLLKIGSKQYYRYIGSLTVPPCTENVIWTMIREVRTVTEEQVKQLRVAVH 240

Query: 241 DDSDTNARPLQPIYDRTMKLQRPKESED 269
           DDSDTNARPLQPIY+R  +LQRPKE+ED
Sbjct: 241 DDSDTNARPLQPIYNRKTQLQRPKENED 268

BLAST of Spg032723 vs. ExPASy TrEMBL
Match: A0A1S4E3N8 (Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103502435 PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 5.2e-124
Identity = 208/243 (85.60%), Postives = 232/243 (95.47%), Query Frame = 0

Query: 25  DDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQSPIDMLHERVEIISHLGDLKINYKP 84
           +DER F+YE+DGEKGP HWGEL+ EWH CK GLMQSPID+LHERVE+ISHLGDL+INYK 
Sbjct: 6   EDERGFDYERDGEKGPVHWGELRAEWHKCKGGLMQSPIDLLHERVEVISHLGDLQINYKS 65

Query: 85  SNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKNFDLEAHLVHES 144
           SNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKNFDLEAHLVHES
Sbjct: 66  SNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKNFDLEAHLVHES 125

Query: 145 SNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYEEERVIGIIDPTLLKIGSKQYYRYM 204
           SNGM+SVIGILYQIGEPDYFLSTIK+ LEV S+T+EEERV+GIIDP +LK+GSKQYYRY+
Sbjct: 126 SNGMVSVIGILYQIGEPDYFLSTIKEDLEVASNTHEEERVVGIIDPNILKMGSKQYYRYI 185

Query: 205 GSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVHDDSDTNARPLQPIYDRTMKLQRPK 264
           GSLTVPPCTE+V WT+IH+VRT+TEEQVKLLRVAVHDDS++NARPLQPIY+RTM+LQR K
Sbjct: 186 GSLTVPPCTEDVMWTIIHQVRTVTEEQVKLLRVAVHDDSNSNARPLQPIYNRTMQLQRSK 245

Query: 265 ESE 268
           +SE
Sbjct: 246 DSE 248

BLAST of Spg032723 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 323.9 bits (829), Expect = 1.2e-88
Identity = 147/259 (56.76%), Postives = 201/259 (77.61%), Query Frame = 0

Query: 5   LCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQSPIDM 64
           L S F  + + S  ++  EV+DEREFNY+K+ EKGP  WGELKPEW  C  G MQSPID+
Sbjct: 15  LFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQSPIDL 74

Query: 65  LHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCHWHSP 124
           ++ERV I+SHLG L  +Y PSNATLKNRGHD+M+K+ DG G +++NG +Y+L+Q HWHSP
Sbjct: 75  MNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLHWHSP 134

Query: 125 SEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYEEERV 184
           SEHTI+G+ F LE H+VHE  N  ++V+ +LY+IG  D F+ +++  LE +++  E E+ 
Sbjct: 135 SEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEMEEAEKN 194

Query: 185 IGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVHDDSD 244
           +G+IDPT +KIGS++YYRY GSLT PPCT+NVTW+++ +VRT+T +QVKLLRVAVHDD++
Sbjct: 195 VGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVHDDAN 254

Query: 245 TNARPLQPIYDRTMKLQRP 264
           +NARP+QP   R + L RP
Sbjct: 255 SNARPVQPTNKRIVHLYRP 273

BLAST of Spg032723 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 280.4 bits (716), Expect = 1.5e-75
Identity = 134/265 (50.57%), Postives = 186/265 (70.19%), Query Frame = 0

Query: 1   MGKLLCSFFFCILLLSFKAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQS 60
           +G +    F  I ++S      EV+DE +FNYEK GEKGP +WG LKPEW  C  G MQS
Sbjct: 6   IGYVFFLIFISITIVSSSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQS 65

Query: 61  PIDMLHERVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDG--GGYMEMNGTQYKLKQ 120
           PID+  +RV I  +LG L+  Y PSNAT+KNRGHD+MMK+  G  G  + +NGT+YKL+Q
Sbjct: 66  PIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQ 125

Query: 121 CHWHSPSEHTIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDT 180
            HWHSPSEHT++GK F LE H+VH+S +G  +V+   Y++G+PDYFL T++ +L+ ++DT
Sbjct: 126 IHWHSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDT 185

Query: 181 YEEERVIGIIDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVA 240
           +E +  + ++ P      SK YYR++GSLT PPC+ENV WT+  E+RT+T +Q+ +LRV 
Sbjct: 186 HESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVT 245

Query: 241 VHDDSDTNARPLQPIYDRTMKLQRP 264
           VHD S++NARPLQ   +R + L  P
Sbjct: 246 VHDQSNSNARPLQRKNERPVALYIP 270

BLAST of Spg032723 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 252.7 bits (644), Expect = 3.4e-67
Identity = 110/204 (53.92%), Postives = 156/204 (76.47%), Query Frame = 0

Query: 24  VDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQSPIDMLHERVEIISHLGDLKINYK 83
           V+DE EF+YE + E GP  WG+L+PEW  C  G MQSPID++++RV +++HL  L  +YK
Sbjct: 5   VEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYK 64

Query: 84  PSNATLKNRGHDMMMKW-VDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKNFDLEAHLVH 143
           P NATLKNRGHDMM+K+  +G G + +NGT+YKL Q HWHSPSEHT++G+ F LE H+VH
Sbjct: 65  PCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALELHMVH 124

Query: 144 ESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYEEERVIGIIDPTLLKIGSKQYYR 203
           E+ NG ++V+ +LY+IG PD FL  +++ L  ++D  E E+ + +IDP  +KIGS+++YR
Sbjct: 125 ENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSRKFYR 184

Query: 204 YMGSLTVPPCTENVTWTMIHEVRT 227
           Y+GSLT PPCT+NV WT++ +V T
Sbjct: 185 YIGSLTTPPCTQNVIWTVVKKVNT 208

BLAST of Spg032723 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 241.9 bits (616), Expect = 5.9e-64
Identity = 117/256 (45.70%), Postives = 165/256 (64.45%), Query Frame = 0

Query: 11  CILLLSF---KAASQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQSPIDMLHE 70
           C + LSF     A  EVDDE  F YE+  EKGP  WG++ P W  C  G  QSPID+ +E
Sbjct: 14  CFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPIDLTNE 73

Query: 71  RVEIISHLGDLKINYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCHWHSPSEH 130
           RV +I H       YKP+ A + NRGHD+M+ W    G M +  T + L QCHWHSPSEH
Sbjct: 74  RVSLI-HDQAWTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHSPSEH 133

Query: 131 TIDGKNFDLEAHLVHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYEEERVIGI 190
           T++G  +DLE H+VH S+ G  +VIG+LY++GEP+ FL+ + + ++ V +   +E  +G+
Sbjct: 134 TVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNGIKAVGN---KEINLGM 193

Query: 191 IDPTLLKIGSKQYYRYMGSLTVPPCTENVTWTMIHEVRTITEEQVKLLRVAVHDDSDTNA 250
           IDP  ++  ++++YRY+GSLTVPPCTE V WT++  V TI+ EQ+  LR AV D  +TN+
Sbjct: 194 IDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDDGFETNS 253

Query: 251 RPLQPIYDRTMKLQRP 264
           RP+Q    R++    P
Sbjct: 254 RPVQDSKGRSVWFYDP 265

BLAST of Spg032723 vs. TAIR 10
Match: AT4G21000.1 (alpha carbonic anhydrase 6 )

HSP 1 Score: 212.2 bits (539), Expect = 5.0e-55
Identity = 94/200 (47.00%), Postives = 143/200 (71.50%), Query Frame = 0

Query: 21  SQEVDDEREFNYEKDGEKGPTHWGELKPEWHSCKAGLMQSPIDMLHERVEIISHLGDLKI 80
           ++E+ ++  F Y++  EKGP  WG+L P+W  C  G +QSPID+  ERV +I H   L  
Sbjct: 28  AREIGNKPLFTYKQKTEKGPAEWGKLDPQWKVCSTGKIQSPIDLTDERVSLI-HDQALSK 87

Query: 81  NYKPSNATLKNRGHDMMMKWVDGGGYMEMNGTQYKLKQCHWHSPSEHTIDGKNFDLEAHL 140
           +YKP++A +++RGHD+M+ W   GG + ++ T YKL QCHWHSPSEHTI+G ++DLE H+
Sbjct: 88  HYKPASAVIQSRGHDVMVSWKGDGGKITIHQTDYKLVQCHWHSPSEHTINGTSYDLELHM 147

Query: 141 VHESSNGMISVIGILYQIGEPDYFLSTIKDHLEVVSDTYEEERVIGIIDPTLLKIGSKQY 200
           VH S++G  +V+G+LY++GEPD FL+ I + ++ V    ++E  +GI+DP  ++  +  +
Sbjct: 148 VHTSASGKTTVVGVLYKLGEPDEFLTKILNGIKGVG---KKEIDLGIVDPRDIRFETNNF 207

Query: 201 YRYMGSLTVPPCTENVTWTM 221
           YRY+GSLT+PPCTE V WT+
Sbjct: 208 YRYIGSLTIPPCTEGVIWTV 223

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152913.17.3e-14189.18alpha carbonic anhydrase 7-like [Momordica charantia][more]
KAG6583572.19.6e-14189.14Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022970331.11.6e-14088.76alpha carbonic anhydrase 7-like [Cucurbita maxima][more]
XP_023520292.14.8e-14088.76alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022964924.16.2e-14088.76alpha carbonic anhydrase 7-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8L8171.7e-8756.76Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1[more]
Q84UV87.3e-7554.05Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS... [more]
F4HUC42.1e-7450.57Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1[more]
Q9FM992.9e-7152.42Alpha carbonic anhydrase 8 OS=Arabidopsis thaliana OX=3702 GN=ACA8 PE=3 SV=3[more]
F4IHR41.5e-6751.79Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1DG523.6e-14189.18Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020526 PE=3 SV=1[more]
A0A6J1I0A87.9e-14188.76Carbonic anhydrase OS=Cucurbita maxima OX=3661 GN=LOC111469326 PE=3 SV=1[more]
A0A6J1HPL03.0e-14088.76Carbonic anhydrase OS=Cucurbita moschata OX=3662 GN=LOC111464882 PE=3 SV=1[more]
A0A6J1DHJ22.4e-13786.19Carbonic anhydrase OS=Momordica charantia OX=3673 GN=LOC111020525 PE=3 SV=1[more]
A0A1S4E3N85.2e-12485.60Carbonic anhydrase OS=Cucumis melo OX=3656 GN=LOC103502435 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08080.11.2e-8856.76alpha carbonic anhydrase 7 [more]
AT1G08065.11.5e-7550.57alpha carbonic anhydrase 5 [more]
AT2G28210.13.4e-6753.92alpha carbonic anhydrase 2 [more]
AT4G20990.15.9e-6445.70alpha carbonic anhydrase 4 [more]
AT4G21000.15.0e-5547.00alpha carbonic anhydrase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001148Alpha carbonic anhydrase domainSMARTSM01057Carb_anhydrase_2acoord: 32..262
e-value: 5.3E-67
score: 238.6
IPR001148Alpha carbonic anhydrase domainPFAMPF00194Carb_anhydrasecoord: 30..259
e-value: 5.3E-42
score: 144.1
IPR001148Alpha carbonic anhydrase domainPROSITEPS51144ALPHA_CA_2coord: 28..262
score: 45.633965
IPR036398Alpha carbonic anhydrase domain superfamilyGENE3D3.10.200.10Alpha carbonic anhydrasecoord: 26..266
e-value: 2.8E-76
score: 258.2
IPR036398Alpha carbonic anhydrase domain superfamilySUPERFAMILY51069Carbonic anhydrasecoord: 29..258
IPR023561Carbonic anhydrase, alpha-classPANTHERPTHR18952CARBONIC ANHYDRASEcoord: 19..265
NoneNo IPR availablePANTHERPTHR18952:SF239BIFUNCTIONAL MONODEHYDROASCORBATE REDUCTASE AND CARBONIC ANHYDRASE NECTARIN-3coord: 19..265
IPR018338Carbonic anhydrase, alpha-class, conserved sitePROSITEPS00162ALPHA_CA_1coord: 125..141
IPR041891Carbonic anhydrase, prokaryotic-likeCDDcd03124alpha_CA_prokaryotic_likecoord: 39..257
e-value: 3.85812E-97
score: 281.853

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg032723.1Spg032723.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0004089 carbonate dehydratase activity
molecular_function GO:0008270 zinc ion binding