Spg032716 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg032716
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Protein NUCLEAR FUSION DEFECTIVE 4
Location	scaffold11: 5749116 .. 5751007 (+)
RNA-Seq Expression	Spg032716
Synteny	Spg032716

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTGTTTCAACAACGTGCAGCAACAATGGCGGAATTAGGATGGGAGAATTGCCGAGTTTTGCTCGGCAAGTGTTGTTGGGGCGGTGGTTTGCCATTTTCGTTTCGTTCCTCGTAATGACCTTCGCCGGCGGATTTTATTTGTTCGGTTATTTCTCCAATGACATCAGAGCAAAGCTCAATTGTGACCAAAGCTCACTCAACCAGATTGGCTTTTACAAAGACCTTGGTTCCAACGTCGGCATTGTCACCGGCCTTCTCTCCGATGTGGCGCCGTCTTGGGTCCTTCTCGTCATCTACGCCACCTTCAACTTCATCGGCTACTTCAAGGTCTGGCAAGGCGTCACCGGACAGGTCCCCAACCCGACCCTTGGCTACTTTTCCCTTTACATGGCCATCGCAGGCAACTCTCAAAGCATAGTCAATACAGGTAAAATCTACTTTTATTACAAGTTTAGTCTTTGAATTTTTGGATTATATTGATTAGACCTGAACTTTAAAAGGTCTCTGACTTATTCTATTTGAAAAATTATGTAATATTTTCAGTGGTGCTAGTGACGAGCGTGAAGAACTTCCCTGAAAGGAGAGGGGTGGTATTGGGCCTTTTGAAGGGCTTCGTGGGCATAGGTGCGGCTGTGCTGATCCAACTTTTCGTCGCCATTTATGGCCGTGATACCAAGACTCTGATTCTGTGGATTGCGTGGTTCCCGACTTTGATTACTTTGATGTTCGTTTTCACAATCAGGGAGATCAAAGTTGTGAAGCACCCCAATGAATTCAGAGTTTTCGTTCAGTTTCTATGGATCTCCATCATTCTGGCTCTGCTTCTCTCTGTCTTGATTTTCGCTCAGAAAAGGGTTGAATTCGATGCAATAGCCGACACGTTAGTCGTGATTGCAATCCTGGGTTTGCTTCTTCTTCCATTGTTGATCTCCATTAGAGAAGAAATTGTTCAGTGGAATCTCAAGAAAATGACCAAAGTTACCAGAATCCAATCGACCCCTCAAACGCCCTTAACCTTAGCGCCGTCTTCCACCAAAGTCCAACAATCAAAGCAACGCAATTCTTGGTTCGCGAACATTTTCGACAAACCCGAAAGAGGAGAAGACTACACGATTCTTCAAGCCATTCTCAGTTTCGACATGCTCATCTTGTACCTCACCATGATAATCGGGATCGGCTCGAGCTTCACGGCCGTGGACAATCTGACCCAGATCGGAGAATCCCAAGGCTACCATACCGAAACCATCAATTCATTCATCTCTGTGGTCAGCATTTCCAACTTCGCCGGCAGAATCTTTTCTGGGTTCGTTTCAGAAATCTTGCTCGAGAAGTACCAATTCCCCCGCCCGTTGATGCTGACCTTCACGCTCTTGGTTTCCTGCATTGGACACTTGTTGGTGGCCTTCCCCTTCCACGGTTCGCTCCTCATGGCATCCATCGTAATTGGATTTGCATTGGGCTCTCAAATTCCCCTGTATTTCGCTATAATTTCAGAGATCTTTGGTCTGAAACACTACTCCCGCTTGTACAATTTTGGGCAACTGTCTTGCCCGATTGGGACTTACGTTCTGAACGTGTTGGTTGCCGGGAATCTCTACGACCAGGAGGTAGAGAAGATGGGGCTTCATTCAAGCGATGGTATATGCATTGGGGATCAGTGTTACAGAAATTCTTTCACAATTTTGACAGCGCTGACGTTCGTGGGAGCTGGAATATCGTTGATCCTGGTGAAGAGAACGGCCGGGTTTTACAGAGGGGATATATACAAGAAGTTCAGAGAAGATATGGAATCTTTGAAGACGGAAGTGGAGCTTTACCCTCTTCCTGTGAAGAGGCCTTCTTCGAGTGTGATCACTATTGACGAACAAAGTACCGATACCAAGAGCTGA

mRNA sequence

ATGATTGTTTCAACAACGTGCAGCAACAATGGCGGAATTAGGATGGGAGAATTGCCGAGTTTTGCTCGGCAAGTGTTGTTGGGGCGGTGGTTTGCCATTTTCGTTTCGTTCCTCGTAATGACCTTCGCCGGCGGATTTTATTTGTTCGGTTATTTCTCCAATGACATCAGAGCAAAGCTCAATTGTGACCAAAGCTCACTCAACCAGATTGGCTTTTACAAAGACCTTGGTTCCAACGTCGGCATTGTCACCGGCCTTCTCTCCGATGTGGCGCCGTCTTGGGTCCTTCTCGTCATCTACGCCACCTTCAACTTCATCGGCTACTTCAAGGTCTGGCAAGGCGTCACCGGACAGGTCCCCAACCCGACCCTTGGCTACTTTTCCCTTTACATGGCCATCGCAGGCAACTCTCAAAGCATAGTCAATACAGTGGTGCTAGTGACGAGCGTGAAGAACTTCCCTGAAAGGAGAGGGGTGGTATTGGGCCTTTTGAAGGGCTTCGTGGGCATAGGTGCGGCTGTGCTGATCCAACTTTTCGTCGCCATTTATGGCCGTGATACCAAGACTCTGATTCTGTGGATTGCGTGGTTCCCGACTTTGATTACTTTGATGTTCGTTTTCACAATCAGGGAGATCAAAGTTGTGAAGCACCCCAATGAATTCAGAGTTTTCGTTCAGTTTCTATGGATCTCCATCATTCTGGCTCTGCTTCTCTCTGTCTTGATTTTCGCTCAGAAAAGGGTTGAATTCGATGCAATAGCCGACACGTTAGTCGTGATTGCAATCCTGGGTTTGCTTCTTCTTCCATTGTTGATCTCCATTAGAGAAGAAATTGTTCAGTGGAATCTCAAGAAAATGACCAAAGTTACCAGAATCCAATCGACCCCTCAAACGCCCTTAACCTTAGCGCCGTCTTCCACCAAAGTCCAACAATCAAAGCAACGCAATTCTTGGTTCGCGAACATTTTCGACAAACCCGAAAGAGGAGAAGACTACACGATTCTTCAAGCCATTCTCAGTTTCGACATGCTCATCTTGTACCTCACCATGATAATCGGGATCGGCTCGAGCTTCACGGCCGTGGACAATCTGACCCAGATCGGAGAATCCCAAGGCTACCATACCGAAACCATCAATTCATTCATCTCTGTGGTCAGCATTTCCAACTTCGCCGGCAGAATCTTTTCTGGGTTCGTTTCAGAAATCTTGCTCGAGAAGTACCAATTCCCCCGCCCGTTGATGCTGACCTTCACGCTCTTGGTTTCCTGCATTGGACACTTGTTGGTGGCCTTCCCCTTCCACGGTTCGCTCCTCATGGCATCCATCGTAATTGGATTTGCATTGGGCTCTCAAATTCCCCTGTATTTCGCTATAATTTCAGAGATCTTTGGTCTGAAACACTACTCCCGCTTGTACAATTTTGGGCAACTGTCTTGCCCGATTGGGACTTACGTTCTGAACGTGTTGGTTGCCGGGAATCTCTACGACCAGGAGGTAGAGAAGATGGGGCTTCATTCAAGCGATGGTATATGCATTGGGGATCAGTGTTACAGAAATTCTTTCACAATTTTGACAGCGCTGACGTTCGTGGGAGCTGGAATATCGTTGATCCTGGTGAAGAGAACGGCCGGGTTTTACAGAGGGGATATATACAAGAAGTTCAGAGAAGATATGGAATCTTTGAAGACGGAAGTGGAGCTTTACCCTCTTCCTGTGAAGAGGCCTTCTTCGAGTGTGATCACTATTGACGAACAAAGTACCGATACCAAGAGCTGA

Coding sequence (CDS)

ATGATTGTTTCAACAACGTGCAGCAACAATGGCGGAATTAGGATGGGAGAATTGCCGAGTTTTGCTCGGCAAGTGTTGTTGGGGCGGTGGTTTGCCATTTTCGTTTCGTTCCTCGTAATGACCTTCGCCGGCGGATTTTATTTGTTCGGTTATTTCTCCAATGACATCAGAGCAAAGCTCAATTGTGACCAAAGCTCACTCAACCAGATTGGCTTTTACAAAGACCTTGGTTCCAACGTCGGCATTGTCACCGGCCTTCTCTCCGATGTGGCGCCGTCTTGGGTCCTTCTCGTCATCTACGCCACCTTCAACTTCATCGGCTACTTCAAGGTCTGGCAAGGCGTCACCGGACAGGTCCCCAACCCGACCCTTGGCTACTTTTCCCTTTACATGGCCATCGCAGGCAACTCTCAAAGCATAGTCAATACAGTGGTGCTAGTGACGAGCGTGAAGAACTTCCCTGAAAGGAGAGGGGTGGTATTGGGCCTTTTGAAGGGCTTCGTGGGCATAGGTGCGGCTGTGCTGATCCAACTTTTCGTCGCCATTTATGGCCGTGATACCAAGACTCTGATTCTGTGGATTGCGTGGTTCCCGACTTTGATTACTTTGATGTTCGTTTTCACAATCAGGGAGATCAAAGTTGTGAAGCACCCCAATGAATTCAGAGTTTTCGTTCAGTTTCTATGGATCTCCATCATTCTGGCTCTGCTTCTCTCTGTCTTGATTTTCGCTCAGAAAAGGGTTGAATTCGATGCAATAGCCGACACGTTAGTCGTGATTGCAATCCTGGGTTTGCTTCTTCTTCCATTGTTGATCTCCATTAGAGAAGAAATTGTTCAGTGGAATCTCAAGAAAATGACCAAAGTTACCAGAATCCAATCGACCCCTCAAACGCCCTTAACCTTAGCGCCGTCTTCCACCAAAGTCCAACAATCAAAGCAACGCAATTCTTGGTTCGCGAACATTTTCGACAAACCCGAAAGAGGAGAAGACTACACGATTCTTCAAGCCATTCTCAGTTTCGACATGCTCATCTTGTACCTCACCATGATAATCGGGATCGGCTCGAGCTTCACGGCCGTGGACAATCTGACCCAGATCGGAGAATCCCAAGGCTACCATACCGAAACCATCAATTCATTCATCTCTGTGGTCAGCATTTCCAACTTCGCCGGCAGAATCTTTTCTGGGTTCGTTTCAGAAATCTTGCTCGAGAAGTACCAATTCCCCCGCCCGTTGATGCTGACCTTCACGCTCTTGGTTTCCTGCATTGGACACTTGTTGGTGGCCTTCCCCTTCCACGGTTCGCTCCTCATGGCATCCATCGTAATTGGATTTGCATTGGGCTCTCAAATTCCCCTGTATTTCGCTATAATTTCAGAGATCTTTGGTCTGAAACACTACTCCCGCTTGTACAATTTTGGGCAACTGTCTTGCCCGATTGGGACTTACGTTCTGAACGTGTTGGTTGCCGGGAATCTCTACGACCAGGAGGTAGAGAAGATGGGGCTTCATTCAAGCGATGGTATATGCATTGGGGATCAGTGTTACAGAAATTCTTTCACAATTTTGACAGCGCTGACGTTCGTGGGAGCTGGAATATCGTTGATCCTGGTGAAGAGAACGGCCGGGTTTTACAGAGGGGATATATACAAGAAGTTCAGAGAAGATATGGAATCTTTGAAGACGGAAGTGGAGCTTTACCCTCTTCCTGTGAAGAGGCCTTCTTCGAGTGTGATCACTATTGACGAACAAAGTACCGATACCAAGAGCTGA

Protein sequence

MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLNQIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYFSLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQTPLTLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFTAVDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSCPIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQSTDTKS

Homology

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BLAST of Spg032716 vs. NCBI nr
Match: XP_004147304.1 (uncharacterized protein LOC101203173 [Cucumis sativus] >KAE8652743.1 hypothetical protein Csa_013832 [Cucumis sativus])

HSP 1 Score: 752.3 bits (1941), Expect = 3.2e-213
Identity = 394/595 (66.22%), Postives = 475/595 (79.83%), Query Frame = 0


Query: 1   MIVSTTC---SNNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIR 60
           M VSTT    +NNGG R  E+ SFA QV+LGRWFA+F SFLVMT AGGFYLF YFS DI+
Sbjct: 1   MAVSTTLPVGNNNGGTRSVEISSFATQVVLGRWFALFASFLVMTGAGGFYLFAYFSKDIK 60

Query: 61  AKLNCDQSSLNQIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTG 120
             L CDQ++LN+IGFYKDLGSN+GI++G +++VAP W LL++ +  NFIGYFK+W+GV G
Sbjct: 61  ETLKCDQTTLNKIGFYKDLGSNIGIISGFMAEVAPPWSLLLLASAVNFIGYFKIWEGVVG 120

Query: 121 QVPNPTLGYFSLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQ 180
           +V NPT+ YF  Y+ + GNSQ + NTVVLVT VKNFPERRGV+LGLLKGF+GIG AVL Q
Sbjct: 121 RVVNPTVEYFCFYITVGGNSQILANTVVLVTCVKNFPERRGVILGLLKGFLGIGGAVLTQ 180

Query: 181 LFVAIYGRDTKTLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALL 240
           +  AIYG +TK++IL IAWFP+LITL+F FTIREI+VVKHPNEFRVF  FL++S+IL   
Sbjct: 181 IHYAIYGHETKSIILLIAWFPSLITLLFAFTIREIRVVKHPNEFRVFFHFLFVSLILPFF 240

Query: 241 LSVLIFAQKRVEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQ 300
           L +LI  Q RV FD +A T VV+AI+GLLL PL I+IREE+VQWNL K+T++ + Q+  Q
Sbjct: 241 LFILIILQGRVHFDQLAYTFVVVAIMGLLLTPLFIAIREELVQWNLTKITQLVKSQTITQ 300

Query: 301 TPLT-LAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGS 360
             LT ++P + K        S+F NIFDKPERGEDYT LQA++S DM ILYLTMIIGIGS
Sbjct: 301 KRLTSISPPTPKT------TSFFENIFDKPERGEDYTFLQAVMSIDMFILYLTMIIGIGS 360

Query: 361 SFTAVDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLT 420
           SFTA+DNL QIGESQ Y TE+I+  IS+ SI NF GRIFSGF SEILLEK++FPRPLMLT
Sbjct: 361 SFTAMDNLAQIGESQRYSTESIDLIISMASIFNFLGRIFSGFASEILLEKFKFPRPLMLT 420

Query: 421 FTLLVSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQ 480
           FTLLVSCIG++LVAFPFH SL +ASI+IGF LGSQIPLYFA+ISEIFGLKHYS LYNFGQ
Sbjct: 421 FTLLVSCIGNILVAFPFHHSLYVASILIGFCLGSQIPLYFAMISEIFGLKHYSLLYNFGQ 480

Query: 481 LSCPIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISL 540
           LSCP+G+Y+LNVLVAG  YD+E + +  +S    C G+ CYRNSF ILT ++ VGA ISL
Sbjct: 481 LSCPVGSYILNVLVAGRFYDEEAKTINGNSIYLTCKGEFCYRNSFAILTGMSLVGAVISL 540

Query: 541 ILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQSTDTKS 592
           ILVKRT  FY+GDIY+KFREDM+SLK+EVELY +  K    SV  +  Q+TDT S
Sbjct: 541 ILVKRTNEFYKGDIYRKFREDMDSLKSEVELYRIDTKSEIDSV-NVCRQTTDTSS 588

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BLAST of Spg032716 vs. NCBI nr
Match: XP_038895749.1 (uncharacterized protein LOC120083913 [Benincasa hispida])

HSP 1 Score: 746.1 bits (1925), Expect = 2.3e-211
Identity = 385/574 (67.07%), Postives = 468/574 (81.53%), Query Frame = 0


Query: 1   MIVST-TCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAK 60
           M+VST   SNNGG+   E  SFARQV++GRWF++F SFLVMT AG F+LF +FS DI+  
Sbjct: 1   MVVSTIPVSNNGGMGTAEFLSFARQVVVGRWFSLFASFLVMTGAGSFFLFPHFSKDIKET 60

Query: 61  LNCDQSSLNQIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQV 120
           L CDQ++LN+IGFYKDLGSNVGI++ LL  + PSW LLV+ +  NFIG+FK+W+ VTG+V
Sbjct: 61  LKCDQTTLNKIGFYKDLGSNVGIISALLGGILPSWCLLVLSSAVNFIGHFKIWEAVTGKV 120

Query: 121 PNPTLGYFSLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLF 180
             PT+ YF  Y A+ GNSQ++ NTVVLVT VKNFPERRGV+LGLLKGF+G+G AVL Q++
Sbjct: 121 VRPTVEYFCFYFAVGGNSQTLANTVVLVTCVKNFPERRGVILGLLKGFLGLGGAVLTQVY 180

Query: 181 VAIYGRDTKTLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLS 240
            AIYG DTK+LIL IAW P+LI+L+F + IRE+ +VKHPNEFRVF QFL +S+ILAL L+
Sbjct: 181 YAIYGHDTKSLILLIAWLPSLISLIFAYAIREVTIVKHPNEFRVFTQFLSVSLILALFLT 240

Query: 241 VLIFAQKRVEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQTP 300
           +LIF Q+ V FD +    VV AI+GLLLLPLLI+IREE+VQWNL K+TK+ +  + PQ  
Sbjct: 241 ILIFLQREVRFDELTHVSVVTAIMGLLLLPLLIAIREELVQWNLNKITKLRKSLTLPQIS 300

Query: 301 LTLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFT 360
           ++ AP       S Q +S+F NIF+KP+RGEDYT+LQAILS DMLILY+TMIIGIGSSFT
Sbjct: 301 IS-APKLN--NPSFQTDSFFQNIFNKPKRGEDYTVLQAILSIDMLILYMTMIIGIGSSFT 360

Query: 361 AVDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTL 420
           A+DN  QIGESQGY  E+++S IS+VSI NF GRIFSGF SEILLEK++FPRPLMLTFTL
Sbjct: 361 AMDNFGQIGESQGYSNESLDSIISIVSIFNFCGRIFSGFASEILLEKFKFPRPLMLTFTL 420

Query: 421 LVSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSC 480
           L+SCIGH+LVAFPFH SL +ASI+ GFALGSQIPLY+A+ISEIFGLKHYS LYNFGQLSC
Sbjct: 421 LISCIGHILVAFPFHHSLYIASIITGFALGSQIPLYYAMISEIFGLKHYSFLYNFGQLSC 480

Query: 481 PIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILV 540
           PIGTYVLNVLVAG  YDQE  K   +SS   C G+ CYRNSF ILT ++ +GA ISLILV
Sbjct: 481 PIGTYVLNVLVAGKFYDQEA-KTSAYSSQFKCEGELCYRNSFVILTGISLLGAAISLILV 540

Query: 541 KRTAGFYRGDIYKKFREDMESLKTEVELYPLPVK 574
           +RT+ FYRGDIYKKFREDM+SLKT+++LY +  K
Sbjct: 541 RRTSEFYRGDIYKKFREDMDSLKTDMKLYAIDCK 570

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BLAST of Spg032716 vs. NCBI nr
Match: XP_008464809.1 (PREDICTED: uncharacterized protein LOC103502605 [Cucumis melo])

HSP 1 Score: 731.5 bits (1887), Expect = 5.8e-207
Identity = 374/596 (62.75%), Postives = 470/596 (78.86%), Query Frame = 0


Query: 1   MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKL 60
           MIV+    +NGGIR GEL  F  QV++GRWF++F SFLVMT AGG YLF Y+S DI+  L
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  NCDQSSLNQIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVP 120
            CDQ++LN+IGFYKDLGSNVG++ GLL++VAP+W++L+I A FNFIGYFK+WQ VTG++ 
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 NPTLGYFSLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFV 180
            PT+ +F  Y+ I  NSQ+  NT VLVT VKNFPERRGV+LGLLKGFVG+  A++ QL++
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGRDTKTLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSV 240
           AIYG DTK+LIL IAWFP++++L+FV+TIREIK VKHPNEFRVFVQFL ++++LALLL+ 
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LIFAQKRVEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTR------IQS 300
           LIF QKRV FD  A   +V AIL LL +PLLI+IREE+V WNL K T ++       I+S
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 TPQTPLTLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGI 360
           +   P   +PS+ +     Q  S F+NIF+KPERGEDYT+LQAI S DMLI+  TM+IG+
Sbjct: 301 SQPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGV 360

Query: 361 GSSFTAVDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLM 420
           G+S TA+DNL QIGE+QGY +ETIN F+S++SISNF GRIFSGFVSEILLEK+QFPRPLM
Sbjct: 361 GASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLM 420

Query: 421 LTFTLLVSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNF 480
           LT  LL+S +GHLLVAFPF  SL +ASI+IGF++GSQ+PL+FA+ISEIFGLKHYS L+NF
Sbjct: 421 LTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNF 480

Query: 481 GQLSCPIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGI 540
           GQLSCPIG+Y+LNV+V G LYD EV ++G + +   C+G  CY  SF IL  LTF+ A I
Sbjct: 481 GQLSCPIGSYILNVMVTGKLYD-EVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMI 540

Query: 541 SLILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQSTDTK 591
           SLILV+RT  FYRGDIYKKF+EDME+LKTEVE Y +  KR     + +D+ S + K
Sbjct: 541 SLILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 595

[Close]

BLAST of Spg032716 vs. NCBI nr
Match: KAA0051898.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ99888.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 728.4 bits (1879), Expect = 4.9e-206
Identity = 371/588 (63.10%), Postives = 466/588 (79.25%), Query Frame = 0


Query: 9   NNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLN 68
           +NGGIR GEL  F  QV++GRWF++F SFLVMT AGG YLF Y+S DI+  L CDQ++LN
Sbjct: 34  SNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQCDQTTLN 93

Query: 69  QIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYFS 128
           +IGFYKDLGSNVG++ GLL++VAP+W++L+I A FNFIGYFK+WQ VTG++  PT+ +F 
Sbjct: 94  RIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRPTVAFFC 153

Query: 129 LYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTK 188
            Y+ I  NSQ+  NT VLVT VKNFPERRGV+LGLLKGFVG+  A++ QL++AIYG DTK
Sbjct: 154 FYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTK 213

Query: 189 TLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRV 248
           +LIL IAWFP++++L+FV+TIREIK VKHPNEFRVFVQFL ++++LALLL+ LIF QKRV
Sbjct: 214 SLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALIFVQKRV 273

Query: 249 EFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTR------IQSTPQTPLTL 308
            FD  A   +V AIL LL +PLLI+IREE+V WNL K T ++       I+S+   P   
Sbjct: 274 HFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESSQPKPPIH 333

Query: 309 APSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFTAVD 368
           +PS+ +     Q  S F+NIF+KPERGEDYT+LQAI S DMLI+  TM+IG+G+S TA+D
Sbjct: 334 SPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAID 393

Query: 369 NLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVS 428
           NL QIGE+QGY +ETIN F+S++SISNF GRIFSGFVSEILLEK+QFPRPLMLT  LL+S
Sbjct: 394 NLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLMLTLILLIS 453

Query: 429 CIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSCPIG 488
            +GHLLVAFPF  SL +ASI+IGF++GSQ+PL+FA+ISEIFGLKHYS L+NFGQLSCPIG
Sbjct: 454 SLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIG 513

Query: 489 TYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILVKRT 548
           +Y+LNV+V G LYD EV ++G + +   C+G  CY  SF IL  LTF+ A ISLILV+RT
Sbjct: 514 SYILNVMVTGKLYD-EVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISLILVQRT 573

Query: 549 AGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQSTDTK 591
             FYRGDIYKKF+EDME+LKTEVE Y +  KR     + +D+ S + K
Sbjct: 574 REFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 620

[Close]

BLAST of Spg032716 vs. NCBI nr
Match: XP_038895750.1 (uncharacterized protein LOC120083915 [Benincasa hispida])

HSP 1 Score: 724.5 bits (1869), Expect = 7.1e-205
Identity = 378/600 (63.00%), Postives = 472/600 (78.67%), Query Frame = 0


Query: 1   MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKL 60
           MIV+   S+NGGI  GEL  F RQV++GRWF++F SFLVM+ AGG Y+F Y+S DI+  L
Sbjct: 1   MIVAAGHSSNGGIGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTL 60

Query: 61  NCDQSSLNQIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVP 120
            CDQ++LN+IGFYKDLGSNVGIV GLL++VAP+W++L+I A  NF+GYFK+WQ VTG++ 
Sbjct: 61  KCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIV 120

Query: 121 NPTLGYFSLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFV 180
           +PT+G+F  ++ +  NSQ+  NT VLVT VKNFPERRGV+LGLLKGFVG+  A++ QL++
Sbjct: 121 HPTVGFFCFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGRDTKTLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSV 240
           AIYG DT++L+L IAWFP+LI+L+FV+TIREIK VKHPNEFRVFVQFL ++I+L++ L+V
Sbjct: 181 AIYGHDTRSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTV 240

Query: 241 LIFAQKRVEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKV----TRIQ-ST 300
           LIF QKRV+FD  A   +V AI  LL +PLLI+IREE+V WN  K T +    TRI+  T
Sbjct: 241 LIFIQKRVQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIET 300

Query: 301 PQTPLTLA-----PSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTM 360
           PQT  T       P  +  Q   Q +S FANIF+KPERGEDYT+LQAI S DMLI+  TM
Sbjct: 301 PQTTTTTTTTTNPPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICSTM 360

Query: 361 IIGIGSSFTAVDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFP 420
           +IG+G+S TA+DNL QIGESQGY + TIN FIS+VSI NF GRIFSGFVSEILLEK++FP
Sbjct: 361 LIGVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFP 420

Query: 421 RPLMLTFTLLVSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSR 480
           RPLMLTF LL+SC+GHLLVAFPF  SL +ASI+IGF++GSQ+PL+FA+ISEIFGLKHYS 
Sbjct: 421 RPLMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYST 480

Query: 481 LYNFGQLSCPIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFV 540
           L+NFGQLSCPIG+Y+LNV+V G LYD EV ++G   +   C G+ CY  SF IL+ LTF+
Sbjct: 481 LFNFGQLSCPIGSYILNVMVTGKLYD-EVAQLGSSPNKFHCQGNHCYEQSFLILSGLTFI 540

Query: 541 GAGISLILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQSTDTK 591
            A ISLILVKRT  FY GDIYKKFREDME+LKTE+E Y L  KR     + +D+ S + K
Sbjct: 541 VAMISLILVKRTREFYGGDIYKKFREDMETLKTEMEFYRLDEKRTRIGNLLVDKHSINFK 599

[Close]

BLAST of Spg032716 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.7e-15
Identity = 123/562 (21.89%), Postives = 230/562 (40.93%), Query Frame = 0


Query: 29  RWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLNQIGFYKDLGSNVGIVTGLLS 88
           +W  +  +  +    G  + F  +S+ +++ L   Q  LN +    DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 89  DVAPSWVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYFSLYMA--IAGNSQSIVNTVVL 148
              P  V+L   A   F+GY   W  +T  +   TL Y  +++   +AG S    NT   
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNII---TLPYSLVFLCCLLAGLSICWFNTACF 162

Query: 149 VTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIAWFPTLITLMFV 208
           +  +++FP  R + L L   F GI AA+    F AI    +   +L  +  P +++   +
Sbjct: 163 ILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAAL 222

Query: 209 F---------TIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDAIADTL 268
           +         T  +    +H +     +  L +     LLLS    +  R+ F       
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIG----- 282

Query: 269 VVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQTPLTLAPSSTKVQQSKQRNS 328
            V+ ++  L  PLL+  R+    + L  +      +S+    L +     +      +  
Sbjct: 283 AVVLLVFPLCAPLLVYARD----YFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTG 342

Query: 329 W----FANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFTAVDNLTQIGESQGY 388
           +     A   +    G++++    I   +  + Y+    G        +NL QI +S G 
Sbjct: 343 YEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQ 402

Query: 389 HTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLVAFPF 448
           ++ T+   +++ S  +F GR+ S    + + ++++  R       LL + I   L+A   
Sbjct: 403 NSTTL---VTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSS 462

Query: 449 --HGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSCPIGTYVLNVLVA 508
               +L  A+ +IG + G       +I S++FG       +N    + PIG+ +L   +A
Sbjct: 463 SQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIA 522

Query: 509 GNLYDQEV--EKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILVKRTAGFYRGD 568
            ++Y+     +   + S   +CIG  CY  +F     L+ +G   SL L  RT       
Sbjct: 523 ASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTK-----P 582

Query: 569 IYKKFREDMESLKTEV-ELYPL 571
           +Y +  +D  SL +   +L PL
Sbjct: 583 VYHRLEQDKVSLTSSYKDLDPL 582

[Close]

BLAST of Spg032716 vs. ExPASy TrEMBL
Match: A0A1S3CMG9 (uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=4 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 2.8e-207
Identity = 374/596 (62.75%), Postives = 470/596 (78.86%), Query Frame = 0


Query: 1   MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKL 60
           MIV+    +NGGIR GEL  F  QV++GRWF++F SFLVMT AGG YLF Y+S DI+  L
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  NCDQSSLNQIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVP 120
            CDQ++LN+IGFYKDLGSNVG++ GLL++VAP+W++L+I A FNFIGYFK+WQ VTG++ 
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 NPTLGYFSLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFV 180
            PT+ +F  Y+ I  NSQ+  NT VLVT VKNFPERRGV+LGLLKGFVG+  A++ QL++
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGRDTKTLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSV 240
           AIYG DTK+LIL IAWFP++++L+FV+TIREIK VKHPNEFRVFVQFL ++++LALLL+ 
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LIFAQKRVEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTR------IQS 300
           LIF QKRV FD  A   +V AIL LL +PLLI+IREE+V WNL K T ++       I+S
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 TPQTPLTLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGI 360
           +   P   +PS+ +     Q  S F+NIF+KPERGEDYT+LQAI S DMLI+  TM+IG+
Sbjct: 301 SQPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGV 360

Query: 361 GSSFTAVDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLM 420
           G+S TA+DNL QIGE+QGY +ETIN F+S++SISNF GRIFSGFVSEILLEK+QFPRPLM
Sbjct: 361 GASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLM 420

Query: 421 LTFTLLVSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNF 480
           LT  LL+S +GHLLVAFPF  SL +ASI+IGF++GSQ+PL+FA+ISEIFGLKHYS L+NF
Sbjct: 421 LTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNF 480

Query: 481 GQLSCPIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGI 540
           GQLSCPIG+Y+LNV+V G LYD EV ++G + +   C+G  CY  SF IL  LTF+ A I
Sbjct: 481 GQLSCPIGSYILNVMVTGKLYD-EVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMI 540

Query: 541 SLILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQSTDTK 591
           SLILV+RT  FYRGDIYKKF+EDME+LKTEVE Y +  KR     + +D+ S + K
Sbjct: 541 SLILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 595

[Close]

BLAST of Spg032716 vs. ExPASy TrEMBL
Match: A0A5D3BP48 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2042G00080 PE=4 SV=1)

HSP 1 Score: 728.4 bits (1879), Expect = 2.4e-206
Identity = 371/588 (63.10%), Postives = 466/588 (79.25%), Query Frame = 0


Query: 9   NNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLN 68
           +NGGIR GEL  F  QV++GRWF++F SFLVMT AGG YLF Y+S DI+  L CDQ++LN
Sbjct: 34  SNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQCDQTTLN 93

Query: 69  QIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYFS 128
           +IGFYKDLGSNVG++ GLL++VAP+W++L+I A FNFIGYFK+WQ VTG++  PT+ +F 
Sbjct: 94  RIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRPTVAFFC 153

Query: 129 LYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTK 188
            Y+ I  NSQ+  NT VLVT VKNFPERRGV+LGLLKGFVG+  A++ QL++AIYG DTK
Sbjct: 154 FYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDTK 213

Query: 189 TLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRV 248
           +LIL IAWFP++++L+FV+TIREIK VKHPNEFRVFVQFL ++++LALLL+ LIF QKRV
Sbjct: 214 SLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALIFVQKRV 273

Query: 249 EFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTR------IQSTPQTPLTL 308
            FD  A   +V AIL LL +PLLI+IREE+V WNL K T ++       I+S+   P   
Sbjct: 274 HFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESSQPKPPIH 333

Query: 309 APSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFTAVD 368
           +PS+ +     Q  S F+NIF+KPERGEDYT+LQAI S DMLI+  TM+IG+G+S TA+D
Sbjct: 334 SPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAID 393

Query: 369 NLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVS 428
           NL QIGE+QGY +ETIN F+S++SISNF GRIFSGFVSEILLEK+QFPRPLMLT  LL+S
Sbjct: 394 NLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLMLTLILLIS 453

Query: 429 CIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSCPIG 488
            +GHLLVAFPF  SL +ASI+IGF++GSQ+PL+FA+ISEIFGLKHYS L+NFGQLSCPIG
Sbjct: 454 SLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPIG 513

Query: 489 TYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILVKRT 548
           +Y+LNV+V G LYD EV ++G + +   C+G  CY  SF IL  LTF+ A ISLILV+RT
Sbjct: 514 SYILNVMVTGKLYD-EVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISLILVQRT 573

Query: 549 AGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQSTDTK 591
             FYRGDIYKKF+EDME+LKTEVE Y +  KR     + +D+ S + K
Sbjct: 574 REFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFK 620

[Close]

BLAST of Spg032716 vs. ExPASy TrEMBL
Match: A0A6J1HLV5 (uncharacterized protein LOC111464764 OS=Cucurbita moschata OX=3662 GN=LOC111464764 PE=4 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 4.4e-200
Identity = 368/587 (62.69%), Postives = 469/587 (79.90%), Query Frame = 0


Query: 1   MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKL 60
           M+VST    +G I   E  SF RQVL GRWF++F SFLVMT AG FYL+ +FS DI+  L
Sbjct: 1   MVVSTIPIYHGRIGTVEFSSFTRQVLGGRWFSLFASFLVMTSAGAFYLYSHFSKDIKETL 60

Query: 61  NCDQSSLNQIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVP 120
            CDQ++LN+IGFYKD+GSN+ I + LL ++APSWVLLVI +  NFIGYFK+WQGV G++ 
Sbjct: 61  QCDQTTLNKIGFYKDIGSNIDIFSVLLVEIAPSWVLLVICSAVNFIGYFKIWQGVVGKIV 120

Query: 121 NPTLGYFSLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFV 180
            P++ YF  Y+A+ GNSQ + N+VVLVT V+NFPERRGV+LGLLKGF+G+GAAVL Q+++
Sbjct: 121 QPSVEYFCFYIAMGGNSQILANSVVLVTCVRNFPERRGVILGLLKGFLGLGAAVLSQIYL 180

Query: 181 AIYGRDTKTLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSV 240
           AIYG DTK+LIL+IAWFP+LITL+F F+I+E+K+VKHP+EFRVF+QFL I++ILA+ + V
Sbjct: 181 AIYGHDTKSLILFIAWFPSLITLIFAFSIKEVKIVKHPHEFRVFIQFLCITLILAMFIIV 240

Query: 241 LIFAQKRVEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQTPL 300
           L   Q+ V+FD  A  +V+IAI+GLL+LPL I+IREE+V+WNL KMTK  + Q++     
Sbjct: 241 LTIIQRIVQFDQSAHIIVMIAIMGLLILPLFIAIREEMVRWNLNKMTKFEKCQTSAS--- 300

Query: 301 TLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFTA 360
            ++ SS+ +Q S  RNS  +NIF+KPERG+DYTILQ   S DML++ LTM IGIGSS TA
Sbjct: 301 IISYSSSTLQHS--RNSDLSNIFNKPERGDDYTILQGFFSIDMLLICLTMSIGIGSSLTA 360

Query: 361 VDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLL 420
           +DNL QIGESQGY  E+IN+ ++++SI NF GRIFSGFVSEILLE+Y+FPRPLMLT TLL
Sbjct: 361 MDNLGQIGESQGYSAESINAIVTIISIFNFLGRIFSGFVSEILLEQYKFPRPLMLTLTLL 420

Query: 421 VSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSCP 480
           VSCIG+LLVAFPF+ SL +ASI+IGFA GSQ+ L   +ISEIFGLKHY+  YN  QLSCP
Sbjct: 421 VSCIGYLLVAFPFNNSLYIASILIGFAFGSQVLLDITVISEIFGLKHYAIFYNIAQLSCP 480

Query: 481 IGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILVK 540
           IGTYVLNVLVAG LYD+EV K  + SS+  C G+QCY++SFTILT ++ +GA ISLILVK
Sbjct: 481 IGTYVLNVLVAGKLYDKEV-KTWIDSSEYKCHGEQCYKDSFTILTGMSLLGAAISLILVK 540

Query: 541 RTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQST 588
           RT  FYRGDIY+KFREDM+SL+ EVELY L V++     + + ++ T
Sbjct: 541 RTREFYRGDIYRKFREDMDSLREEVELYSLDVRQNEDESVHVHKEVT 581

[Close]

BLAST of Spg032716 vs. ExPASy TrEMBL
Match: A0A6J1I3Q6 (uncharacterized protein LOC111469393 OS=Cucurbita maxima OX=3661 GN=LOC111469393 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 9.1e-198
Identity = 359/571 (62.87%), Postives = 451/571 (78.98%), Query Frame = 0


Query: 17  ELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLNQIGFYKDL 76
           +  +FARQV++GRWF++F SFLVMT AG FY + YFS  I+  L CDQ++LN+I FYKD+
Sbjct: 5   QFSNFARQVVVGRWFSLFASFLVMTSAGAFYFYSYFSEHIKETLQCDQTTLNKISFYKDI 64

Query: 77  GSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYFSLYMAIAGN 136
           GSN+ IV+ LL  +APSWVLL+I +  NF+GYFK+WQ V GQV +PT+ YF +Y+A+AGN
Sbjct: 65  GSNIDIVSALLVCIAPSWVLLLICSALNFVGYFKIWQAVVGQVVHPTVEYFGVYIAMAGN 124

Query: 137 SQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIAW 196
           SQ + NTVVLV  V+NFPERRGV+LGLLKGF+G+GAAVL Q+++AIYG DTK+LIL+IAW
Sbjct: 125 SQILANTVVLVICVRNFPERRGVILGLLKGFLGLGAAVLTQVYLAIYGHDTKSLILFIAW 184

Query: 197 FPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDAIADT 256
            P+LITL+F F+IRE+K+VKHP+EFRVF+Q L I+++LAL L+VL  A++ V+FD +A T
Sbjct: 185 CPSLITLLFAFSIREVKIVKHPHEFRVFIQILCITLVLALFLTVLTIAEQMVQFDQLAYT 244

Query: 257 LVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQTPLTLAPSSTKVQQSKQRN 316
            VV+AI+GLL+LPL I+IREE+VQ NL  MTK  + +S PQ P+               N
Sbjct: 245 FVVVAIIGLLILPLFIAIREEMVQLNLNNMTKFEKSRSKPQIPIHAIS-----HNQNHGN 304

Query: 317 SWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFTAVDNLTQIGESQGYHTE 376
           S+++NIF+KPERG+DYTILQ I S DML++ L M IG GSS TA+DNL QIGESQGY TE
Sbjct: 305 SYYSNIFNKPERGDDYTILQGIFSIDMLLICLAMSIGTGSSLTAMDNLGQIGESQGYSTE 364

Query: 377 TINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLVAFPFHGS 436
           ++N+ +S++SI NF GRIFSGFVSEILLEKY+FPRPLMLT TLLVSCIG LLVAFPFH S
Sbjct: 365 SVNAMVSIISIFNFLGRIFSGFVSEILLEKYKFPRPLMLTLTLLVSCIGFLLVAFPFHNS 424

Query: 437 LLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSCPIGTYVLNVLVAGNLYD 496
           L +ASI+IGFA GSQ+ L   +ISE FGL+HY+  YN  QL+CPIGTYVL VLVAG LYD
Sbjct: 425 LYIASILIGFAFGSQVLLDITMISEFFGLEHYAMFYNVAQLACPIGTYVLKVLVAGKLYD 484

Query: 497 QEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILVKRTAGFYRGDIYKKFRE 556
           +EV K    S + IC G+QCYRNSFT LT ++ +GA ISLILVKRT  FYR DIYKKFRE
Sbjct: 485 EEV-KTWSDSPEYICHGEQCYRNSFTFLTEMSLLGAAISLILVKRTREFYRQDIYKKFRE 544

Query: 557 DMESLKTEVELYPLPVKRPSSSVITIDEQST 588
           DM+SLK EVELYP  V++P    + + ++ T
Sbjct: 545 DMDSLKEEVELYPHDVRQPEVESVHVHKEVT 569

[Close]

BLAST of Spg032716 vs. ExPASy TrEMBL
Match: A0A6J1HYZ1 (uncharacterized protein LOC111469392 OS=Cucurbita maxima OX=3661 GN=LOC111469392 PE=4 SV=1)

HSP 1 Score: 697.6 bits (1799), Expect = 4.5e-197
Identity = 374/596 (62.75%), Postives = 450/596 (75.50%), Query Frame = 0


Query: 1   MIVSTTCSNNGGIRMGELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKL 60
           MIVS T S+N GI  GEL SF RQV+ GRWF++F SFLVM  AGG YLF Y+S DI+  L
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSTDIKTTL 60

Query: 61  NCDQSSLNQIGFYKDLGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVP 120
            CDQ++LN+IGFYKDLGSNVGIV GLL++VAP+WVLL+I A  NF+GYFK+WQGVTG++ 
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 NPTLGYFSLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFV 180
           +PT+ YF  Y+ +  NSQ+  NT VLVT VKNFPERRGV+LGL KGFVG+  A++ Q++ 
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGRDTKTLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSV 240
           AIYG DT++LIL + W P+LI+L+F+ TIREIK VKHPNEFRVFV FL +S+ILAL L+ 
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFAQKRVEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMT-KVTRIQSTPQTP 300
           LIF QKR+ FD  A   VV AI  LLLLPLLI+IREEIV WNL K T  +   Q++P  P
Sbjct: 241 LIFIQKRIRFDQSAHAAVVAAIAALLLLPLLIAIREEIVLWNLNKRTIGIHPPQASPNPP 300

Query: 301 LTLAPSSTKVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFT 360
               PSS+                +KP RGED+TI QAI S DMLIL +TM+IG+G+S T
Sbjct: 301 ----PSSS----------------NKPSRGEDHTIPQAIFSADMLILCITMLIGVGASLT 360

Query: 361 AVDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTL 420
           A+DNL QIGES GY +ETINS I +VSI NF GRIFSGFVS+ILLEK++FPRPLMLT  L
Sbjct: 361 AIDNLRQIGESHGYPSETINSLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLVL 420

Query: 421 LVSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSC 480
           LVSCIGHLLVAFPFHGSL +ASIVIGF+LG+Q+PL+FA+ISE+FGLKHYS L+NFGQLSC
Sbjct: 421 LVSCIGHLLVAFPFHGSLYIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLSC 480

Query: 481 PIGTYVLNVLVAGNLYDQEVEKM----GLHSSDGICIGDQCYRNSFTILTALTFVGAGIS 540
           PIG+Y+LNV+V G LYD+  +      GLH     C G  CY  SF IL  LTF  A +S
Sbjct: 481 PIGSYILNVMVTGRLYDEMAKATSGIGGLH-----CEGHSCYEQSFVILAGLTFFVALVS 540

Query: 541 LILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQSTDTKS 592
           LILV RT  FYRGDIYKKFREDMESLKT++E Y L  KR     + +D+ S + KS
Sbjct: 541 LILVGRTREFYRGDIYKKFREDMESLKTKMEFYTLDDKRTRIGNLLVDKHSINFKS 571

[Close]

BLAST of Spg032716 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 535.0 bits (1377), Expect = 7.5e-152
Identity = 291/554 (52.53%), Postives = 385/554 (69.49%), Query Frame = 0


Query: 28  GRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLNQIGFYKDLGSNVGIVTGLL 87
           GRWF +F SFL+M  AG  YLFG +S DI++ L  DQ++LN +GF+KDLG+NVG+++GL+
Sbjct: 16  GRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANVGVLSGLI 75

Query: 88  SDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYFSLYMAIAGNSQSIVNTVVLV 147
           ++V P+W +L I +  NF+GYF +W  VTG+V  P +    LY+ I  NSQ+  NT  LV
Sbjct: 76  AEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNFANTGALV 135

Query: 148 TSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIAWFPTLITLMFVF 207
           T VKNFPE RGV+LGLLKG+VG+  A+  QL+ AIYG D+K+LIL IAW P  ++L+FV+
Sbjct: 136 TCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAAVSLVFVY 195

Query: 208 TIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDAIADTLVVIAILGLLL 267
            IRE KVV+  NE  VF QFL+ISI LAL L  +  A+K+V F   A          LL 
Sbjct: 196 LIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASATICCALLF 255

Query: 268 LPLLISIREEIVQWNLKKM--TKVTRIQ-STPQTPLTL-APSSTKVQ-QSKQRNSWFANI 327
           +PL +S+++E+  WN+ K+   + + ++   P+  L L    + KV  + K+  S F+ +
Sbjct: 256 VPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKETKSCFSTV 315

Query: 328 FDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFTAVDNLTQIGESQGYHTETINSFI 387
           F  P RGEDYTILQA+LS DM+IL++    G+GSS TAVDNL QIGES GY   T++SF+
Sbjct: 316 FSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPNHTVSSFV 375

Query: 388 SVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLVAFPFHGSLLMASI 447
           S+VSI N+ GR+FSGFVSE LL KY+ PRPLM+T  LL+SC GHLL+AFP  GS+ +ASI
Sbjct: 376 SLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPGSVYIASI 435

Query: 448 VIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSCPIGTYVLNVLVAGNLYDQEVEKM 507
           ++GF+ G+Q+PL FAIISE+FGLK+YS L+N GQL+ P+G+Y+LNV V G LYD+E  K 
Sbjct: 436 LMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLYDKEALKQ 495

Query: 508 ----GLHSSDG---ICIGDQCYRNSFTILTALTFVGAGISLILVKRTAGFYRGDIYKKFR 567
               GL   D     C+G QCY+  F IL A+TF GA +SL L  RT  FY+GDIYKKFR
Sbjct: 496 LTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKGDIYKKFR 555

Query: 568 EDMESLKTEVELYP 570
           E  ES   E EL P
Sbjct: 556 ESPES---ESELVP 566

[Close]

BLAST of Spg032716 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 491.5 bits (1264), Expect = 9.5e-139
Identity = 274/596 (45.97%), Postives = 378/596 (63.42%), Query Frame = 0


Query: 16  GELPSFARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLNQIGFYKD 75
           G + S   Q+L GRWF  F S L+M+ AG  Y+FG +S DI+  L  DQ++LN + F+KD
Sbjct: 8   GSMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKD 67

Query: 76  LGSNVGIVTGLLSDVAPSWVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYFSLYMAIAG 135
           LG+NVG++ GLL++V P W +L+I A  NF GYF +W  VT ++  P + +  LY+ +  
Sbjct: 68  LGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 127

Query: 136 NSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDTKTLILWIA 195
           NSQS  NT  LVT VKNFPE RGVVLG+LKG+VG+  A++ QL+ A YG DTK LIL I 
Sbjct: 128 NSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIG 187

Query: 196 WFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDAIAD 255
           W P +++  F+ TIR +KV +  NE +VF  FL+IS+ LA  L V+I   K   F     
Sbjct: 188 WLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 247

Query: 256 TLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQTPLTLAPSSTKVQQSKQR 315
                 ++ LLLLP+++ I EE      KK+ K  ++      P+ +     K+  S+ +
Sbjct: 248 GGSAAVVIVLLLLPIIVVILEE------KKLWKEKQVALNDPAPINVVTEKPKLDSSEFK 307

Query: 316 N----------------SWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFT 375
           +                S +  +F+ PERG+DYTILQA+ S DMLIL+L  I G+G + T
Sbjct: 308 DDDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLT 367

Query: 376 AVDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTL 435
           A+DNL QIG S GY   ++++F+S+VSI N+ GR+ SG VSEI L KY+FPRPLMLT  L
Sbjct: 368 AIDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVL 427

Query: 436 LVSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSC 495
           L+SC GHLL+AF   G L +AS++IGF  G+Q PL FAIISEIFGLK+YS LYNFG ++ 
Sbjct: 428 LLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVAS 487

Query: 496 PIGTYVLNVLVAGNLYDQE-------VEKMGLHSSDGICIGDQCYRNSFTILTALTFVGA 555
           PIG+Y+LNV VAG LYD E       + K  +   D  CIG  C++ SF I+ A+T  G 
Sbjct: 488 PIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGV 547

Query: 556 GISLILVKRTAGFYRGDIYKKFREDMESLKTEVELYPLPVKRPSSSVITIDEQSTD 589
            +S++LV RT  FY+ DIYKKFRE  ++L  E+E+       P+++  T+ ++  D
Sbjct: 548 LVSMVLVIRTKKFYKSDIYKKFRE--KALAAEMEM-----AAPAAARSTVAKEDKD 590

[Close]

BLAST of Spg032716 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 273.9 bits (699), Expect = 3.1e-73
Identity = 176/546 (32.23%), Postives = 299/546 (54.76%), Query Frame = 0


Query: 22  ARQVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLNQIGFYKDLGSNVG 81
           A ++L  +W A+  S  +   AGG Y FG +S  +++  + DQS+L+ +  +KD+G NVG
Sbjct: 2   ATEILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVG 61

Query: 82  IVTGLLSDVAP--------------SWVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYF 141
           +++GL+   A                WV+++I A  NF GYF +W  VTG +  P +   
Sbjct: 62  VLSGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVM 121

Query: 142 SLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDT 201
            L+M IA  S + +NT  +V+S++NF +  G  +G++KGFVG+  A+LIQL+  +   D 
Sbjct: 122 CLFMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDP 181

Query: 202 KTLILWIAWFPTLITLMFVFTIREIKVVKHPNEFRVFVQFLWISIILALLLSVLIFAQKR 261
           KT IL +A  P+L++++ +  +R  K     +E +       +S+I+A  L + I  +  
Sbjct: 182 KTFILLLAIVPSLLSVLVMPLVRVYK-TSTVDEKKHLDGLSTLSLIIAAYLMITIILKST 241

Query: 262 VEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQTPLTLAPSST 321
           +   + A+ + +  +L LL  PLL+++R             + +  S+  +PL       
Sbjct: 242 LSLPSWANAVTLAVLLVLLSSPLLVAVRAH--------RDSIEKPLSSVYSPL------- 301

Query: 322 KVQQSKQRNSWFANIFDKPERGEDYTILQAILSFDMLILYLTMIIGIGSSFTAVDNLTQI 381
            V   +   S    + D+    +   +LQA+ + D  +L+L MI G+GS  + ++N+ QI
Sbjct: 302 -VDNLEATTSGEILMLDE---DKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQI 361

Query: 382 GESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHL 441
           GES  Y +  INS +++ +I NF GR   G+VS+ LL +  +PRPL++  TL    IGHL
Sbjct: 362 GESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHL 421

Query: 442 LVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSCPIGTYVLN 501
           ++A  F G+L   SI++G   GSQ  L   I SE+FG+KH   +YN   ++ P+G+Y+ +
Sbjct: 422 IIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFS 481

Query: 502 VLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILVKRTAGFYR 554
           V + G +YD+ +   G       C G  C+R ++ ++ ++ F+G  +S +LV RT   YR
Sbjct: 482 VRLIGYIYDRTIIGEG-----NTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYR 521

[Close]

BLAST of Spg032716 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 272.7 bits (696), Expect = 6.9e-73
Identity = 179/554 (32.31%), Postives = 299/554 (53.97%), Query Frame = 0


Query: 24  QVLLGRWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLNQIGFYKDLGSNVGIV 83
           ++L  +W A+  S  +   +G  Y FG +S  +++  + DQS+L+ +  +KD+G+N G+ 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 84  TGLLSDVAPS----------------WVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYF 143
           +GLL   A S                WV+L + A   F GYF +W  VTG +  P +   
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 144 SLYMAIAGNSQSIVNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRDT 203
            L+M +A  SQ+  NT  +V++V+NF +  G  +G++KGF+G+  A+LIQL+  +   D 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 204 KTLILWIAWFPTLITLMFVFTIR--EIKVV---KHPNEFRVFVQFLWISIILALLLSVLI 263
            + IL +A  PT+++L+ +  +R  E  V    KH N          +S+I+A  L ++I
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLSA------VSLIIAAYLMIII 241

Query: 264 FAQKRVEFDAIADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQTPLTL 323
             +      + A+ + ++ +L +L LPLLI+ R +           + +      +PL  
Sbjct: 242 ILKNTFGLSSWANIVTLVCLLVMLALPLLIARRAQ--------RDGMEKTVPHDYSPLIS 301

Query: 324 AP-SSTKVQQSKQRNSWFANIFDKPERG--EDYTILQAILSFDMLILYLTMIIGIGSSFT 383
           +P ++T   QS + +S       K E G  E+  +LQA+      +L+L MI G+GS  +
Sbjct: 302 SPKATTSGNQSSEGDS-------KVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGLS 361

Query: 384 AVDNLTQIGESQGYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTL 443
            ++N+ QIGES  Y +  INS +S+ SI NF GR  +G+ S+ LL K  +PRPL++  TL
Sbjct: 362 TINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATL 421

Query: 444 LVSCIGHLLVAFPFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSC 503
               IGHL++A  F G+L + S+++G   GSQ  L   I SE+FG++H   ++N   ++ 
Sbjct: 422 GTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVAS 481

Query: 504 PIGTYVLNVLVAGNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILV 554
           PIG+Y+ +V + G +YD+     G       C G  C+R SF I+ ++ F G  ++++L 
Sbjct: 482 PIGSYIFSVRLIGYIYDKTASGEG-----NTCYGSHCFRLSFIIMASVAFFGFLVAIVLF 529

[Close]

BLAST of Spg032716 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 260.8 bits (665), Expect = 2.7e-69
Identity = 176/542 (32.47%), Postives = 285/542 (52.58%), Query Frame = 0


Query: 29  RWFAIFVSFLVMTFAGGFYLFGYFSNDIRAKLNCDQSSLNQIGFYKDLGSNVGIVTGLLS 88
           +W A   S  + +F+G  Y F  +S+ +++  + DQS+L+ +  +KD+G   GI++G L 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 89  DVAPS--------WVLLVIYATFNFIGYFKVWQGVTGQVPNPTLGYFSLYMAIAGNSQSI 148
               S        WV++ +     F+G+F +W  V G +  P +    L++ +AG+S   
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 149 VNTVVLVTSVKNFPERRGVVLGLLKGFVGIGAAVLIQLFVAIYGRD--TKTLILWIAWFP 208
            NT  +VT+ +NF +  G  +G+++GF+G+  A+LIQL+ A+ G +    T IL +A  P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 209 TLITLMFVFTIREIKVV-----KHPNEFRVFVQFLWISIILALLLSVLIFAQKRVEFDAI 268
           TL+  + +  +R  + V     KH +          IS+I+A  L V+I  +  +     
Sbjct: 187 TLVMFLAMPFVRVYETVTISDKKHLDGLSA------ISMIIAAYLMVVITVENVLGLSRS 246

Query: 269 ADTLVVIAILGLLLLPLLISIREEIVQWNLKKMTKVTRIQSTPQTPLTLAPSSTKVQQSK 328
                 I +L LL  PLL+++R        K+ T  +       T   L P S       
Sbjct: 247 MQIFSFILVLLLLASPLLVAVR----ALREKRQTLSSLDGPVLDTSALLDPPS------- 306

Query: 329 QRNSWFANIFDKPER--GEDYTILQAILSFDMLILYLTMIIGIGSSFTAVDNLTQIGESQ 388
                 +NIF   +    ED  IL+A+ + +  +L+L M+ G+GS F  V+N+ QIGES 
Sbjct: 307 ------SNIFPDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESL 366

Query: 389 GYHTETINSFISVVSISNFAGRIFSGFVSEILLEKYQFPRPLMLTFTLLVSCIGHLLVAF 448
            Y +  +NS +S+ SI NF GR  +G+VS+  L K+ +PRP+ +  TL V  IGH++VA 
Sbjct: 367 RYSSVQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVAS 426

Query: 449 PFHGSLLMASIVIGFALGSQIPLYFAIISEIFGLKHYSRLYNFGQLSCPIGTYVLNVLVA 508
              GSL   S++IG A GSQ  L   I SEIFG++H   +Y    ++ PIG+Y+L+V V 
Sbjct: 427 GVQGSLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVI 486

Query: 509 GNLYDQEVEKMGLHSSDGICIGDQCYRNSFTILTALTFVGAGISLILVKRTAGFYRGDIY 554
           G  YD+   +      D  C G QC+R SF I+ ++   G+ ++ +L  RT  FY+  + 
Sbjct: 487 GYFYDKVASE-----DDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVA 520

The following BLAST results are available for this feature:

BLAST of Spg032716 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147304.1	3.2e-213	66.22	uncharacterized protein LOC101203173 [Cucumis sativus] >KAE8652743.1 hypothetica...	[more]
XP_038895749.1	2.3e-211	67.07	uncharacterized protein LOC120083913 [Benincasa hispida]	[more]
XP_008464809.1	5.8e-207	62.75	PREDICTED: uncharacterized protein LOC103502605 [Cucumis melo]	[more]
KAA0051898.1	4.9e-206	63.10	protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ99888.1 protei...	[more]
XP_038895750.1	7.1e-205	63.00	uncharacterized protein LOC120083915 [Benincasa hispida]	[more]

BLAST of Spg032716 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	1.7e-15	21.89	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]

BLAST of Spg032716 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CMG9	2.8e-207	62.75	uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=...	[more]
A0A5D3BP48	2.4e-206	63.10	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1HLV5	4.4e-200	62.69	uncharacterized protein LOC111464764 OS=Cucurbita moschata OX=3662 GN=LOC1114647...	[more]
A0A6J1I3Q6	9.1e-198	62.87	uncharacterized protein LOC111469393 OS=Cucurbita maxima OX=3661 GN=LOC111469393...	[more]
A0A6J1HYZ1	4.5e-197	62.75	uncharacterized protein LOC111469392 OS=Cucurbita maxima OX=3661 GN=LOC111469392...	[more]

BLAST of Spg032716 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28120.1	7.5e-152	52.53	Major facilitator superfamily protein	[more]
AT2G39210.1	9.5e-139	45.97	Major facilitator superfamily protein	[more]
AT1G18940.1	3.1e-73	32.23	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT1G74780.1	6.9e-73	32.31	Nodulin-like / Major Facilitator Superfamily protein	[more]
AT2G34355.1	2.7e-69	32.47	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 322..546 e-value: 2.7E-13 score: 51.7
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 30..545
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 339..538 e-value: 3.8E-10 score: 39.2
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 29..274 e-value: 2.1E-64 score: 217.4
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 14..562
None	No IPR available	PANTHER	PTHR21576:SF29	PROTEIN NUCLEAR FUSION DEFECTIVE 4	coord: 14..562

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg032716.1	Spg032716.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity