Spg032675 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg032675
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	dTMP kinase
Location	scaffold11: 6396666 .. 6401220 (+)
RNA-Seq Expression	Spg032675
Synteny	Spg032675

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGGAAAAGCTCTGTTTTGGTGCTGTTGACATCTGCGATAGTTGCGAGTTCTATTTCTGGTACTTAATTGACTTGGGGATAAAACTTTTGAGCATTCAATTTGACACAGTTTTAAGGTCAGGTTATGATCCATGGTCGCTTCCACATTCCATAAGCTATTGTAAGATTAAATTCTTGTTAATGTTTATTATTATATTTTTATTTTTGTTTTGAGCTACGACTGTTGGGTTAGATATATATATATATATTTTAAATCAAATGCATCGGATAGTTTCTGGAAATGATTGTGTAGAAGACCTTTCTGATGTTCGTTTTTCCTTTTTAGGAGTTTTGGAGCAGTAACAGCAAGAAGATCCTCGAGTTTGCTACTGAAACTTTCTTCTAAGAATTTTATCGGAAGGATCCAAATGGGAACTAATCAAAACTCTATACTGAGAGGTTGCAGCAGTAACTCTAGAGGAGCCTTGGTGGTTCTTGAAGGTTTGGACCGTTGTGGGAAGACTACACAGTCTAGTAGATTAGTACAGTACTTAGAAGGACTTGGGCATTCTGCTGAACTATGGCGGTTTCCTGACAGAACTACAAGTGTTGGACAAATGATCTCTTCCTATCTTTCAAACAGATCACAACTGGATGACCACACAATACATCTTCTTTTCAGTGCCAATCGATGGGAGAAGAGGTTTGTCAATTTTTCTTTAGACAACTTGCATTTTAGTCCCTTAGATGGATACATTTTCAGTTTCTGATCTGTTTTGTTATCAAGAGGCAAGCTTTTCTTAAAAATTAAAAACAGTAAGACCTGGTTTTAGAAGTTTAAAGTTATGGTAGTTGTGAACTTGCGACCCATAACCATTTTGTTGGATGTTGTGCACTGAGGGAAAATCTGTATATTATATACCTTTTTACAGACGAAAGAATAGTAAATATCTAGCCCAAGAATATGTGATTTGTATTTAATTACTGTAATTCCCATGCAGATCGTTGATGGAAACTAAATTGAAGAGTGGAACCACTCTTATTGTTGATCGGTATTCTTATTCAGGGGTGGCCTTTTCATCTGCCAAAGGACTTGGCATTGAATGGTGTAAGGTAAAAATACAAACCAGCAATTTGGCTGGATCCTTTGTTTATTTCTATCATTTTTTTTAAAAAATTGAAATCCTGATGATATGTTTGCCCTTTGCAGGCACCGGAGGTTGGCTTGCTTGCACCGGACTTAGTACTGTACCTAGACATATCTCCAGAGGTTGGTCAAATTATTATATCATTTTTATGGAAGAATTTTAAATGCATTTATTGGGATGGTTTACCATAATTTATCTTTTTCTTGTAAATATGTAATGTAGCTATGTTTTTTTTTAAAAATTATTGGTCACTTATGGACTACTTTCTTATATTGAATCAATGACTTTTGTACTGATTTTATGATACAATCTCATTCCATTTGTCTATATATATATATATATTTTAATTCGAGTTTTCTATCCATAACATTTTTGCATATCACCTAATACCTGATTTAAACCACCATGTCATAGCCGATAAAACAACTTTCAACCATGTCTTTAAAGAATACAAAAGCCATTGTAAATTTATATTGGGTTTTTCGATGGAACTCAATCAATTGTATTGTTGGCAACATACAATTAGCCCTTGCTCAAGCAGAACAGGTGTTATAAACGAGATTAGTGTCCTTTGTGCAATCTTTGATGTCAAAATATTATACTATTTGCGTGACTTTATCTGGAAGTGAAGCCTCTTTTTACTTCCACCCCCACCCACAAATATTCAAGTTTAATAACTTACCATCTGTATTATGATCGCTTTATGAATAGACTTATTTTGAATGATCAGAAAGCTGCAGAGAGAGGAGGATATGGATGTGAGAGATATGAGCAGCTTGAGTTCCAGAGCAAAGTTGCCCAGTACTATCAAGTTTTGCGTGATTCTTCATGGAAGGTAACTTGTTTTTCATTCGTATCTCAGAAACATTGACATTCAAAGACTAGAGGTTTGGTGTGGTATAGGTCTAGGATGTCTAGATATGTGGACACTCCTTGGGCACATGTCCAACACCTAGATAAGTATCTAGGTAATTGGTTATTTTTTTCCAAACACGGTTAAACATGGCTTGCACACTTTTTGGGGAAATTAGATAAATTCAATCTCTCCCTCAACTTTCAGGGACTCTTTGAAAATCCTTCAAAAGAGATAGACTACTTCAAAAGTTTGTTTATACCAGATGGAAGTCCCGGATCCAAGGCCTAGATTGGAATTATATCAATAGTAATTGGAGTCAATGGCTTGAAAGACCTTTCTGGGATGTGTAAGTAGTGGGAGTGGTTAAAGACCTTGGCAATTTCAAATATGAATAGGCCAAGATGGTTTCTTAGCAAAATTTTTGTAAAAGGCTTGGAATATTATCAGAATCAATTTGTTTAAGGTGTTACATGGGTTGCTCGAAAATGGTATCATAAATGGAAGCACATATAGACTTATCCCAAGAATAGGGTGCCTGAAAATGTGAAAGACTTTAGACCTATAAGTTCAATTGCATCACTTTGCATATGACAATTGCAACTGGTATGGTCATGTCGAGGAAATGGTTGAGAATTGTCTATGTATTTAATAAGATAGAAAGTTGAGGAATCCATTGCTTTGAGATTTCAGGTTCATCTTAGTGGAAGCTGGTTCTTCATATTAAAATTAAAAAAAGATTGTCTGACTGCCTTTTGGACCTTACTTAATTTAATAGCAGATTATTGATGCTTGCCAACCCATGGAAGATATTGAGAAACAACTTCAAGAGATTGTACTGGGCTGTGTCAAGAGATGCCACAGTGGTGTACCGCTTTTTCCCAGCTTTGGTTAAATTGATGCATCTTTTTTTTTTTTCTTCATTTTTTCCCGAGTGTATTTATATTCAACATGTTGAGAACCTGGGGGCTTGTGGTGTGTGTTTTACGTTTCTTTCAACATTTTTATAAGTTTAATTCAACTTTTCTTTTTTCGCAATGTTTTAATTTTCAAGCATTATAGACAATATGAAGCAATGTGATTCAGGAAAGAAAAGTTCAATCACTACACTTCTATCAAACTTGTGCATTTGGGAATACAGAAAATATTACATTTTTTAAATTATTAATCAGAGCAAGACTAATTCATTCTAGTCTTGAACGTTTTGAGTGGTATCTAGTCCACATGTAGAAGTTTCATGCTTTGTAGACTGCCATCCATAATGTAATAATCAAGGGTACAAACATTTGAAGTCAAACATTACATACAACTTTTATGATAGTAAAAAACTGTTATGGTCAACATAGTTGATAGCTGCTAGTTACTGCAATTCAAAAAGAAGGATTCAGCTCCCTAGATCGTGATGGAATCATGGAGACTGAAATGGAAATGTGAAAGGTGGTTCAGGTGGCAGTGGCACCAATGGCTCATTGAAACCAGTATCTGGTGTTGGTGGTGTAGACACAAAGGGAAATGGTTCCTCTGGAATATCTGGAACTTCTGGAACCAGGGGAGGTGGAAGAAAGGTAATGGGAGGCTGTGGAATTGTGAAAGGCGGTGGGTCGGTTGGAATTGATACCAGTGGCGGAGGAAATATAGGGATAAACGGTGGTTGATCTGGTGTGAAATCAGTAGGAGGCGGCAGATCAGGCACTGGTGGAGGTAGTGAAAATTCTGGCAAAGGTGGTGAGAATGTTACTAAAGGTGTGGGAGTGGATAATAATGGTGGTGGTGAAGGAACCAGAGGTGGTGGAAACCAGGTTGAAGGTGTTGGGAGTAGAAAATAATGGTGGTGGTGAAGGAACCAAAGGTGGTGGAACCATTTCTGAAGCGATTGGAGTAGATAGTAATGTTGGTGGTGGTGGTGAATGAACCAAGGGTGGTGGGGGAGATGAAGGAGGTGGAGATGGAGTTTCAGGTGAAGTCAAAGGCGGCGGTGGTGGCGATGTTGCAAGAAGGGGAGGCGGAGAAGGAACTAAGGGGGCTGGCTGATAAACTGGAGGAGGAGAAGAAACTAAAGGGGGCAGAGGAGGAAGTCTTTAGGGTGCCAAAAATGGGTTATCTGAAGGGGAGGTTGGAAAAGGCCATTGGCCACAGGCTCCAAATAAGTACTGGTAAAATGGATCACAATTCGATCTTCTTCTTCCACCACTGCCTCTTCTTCTTTCTCTGCTTCTGCTTCTGATTCTGGTTCTCTTCTCTTCATCTTTCCACCCTTTTCTGTCACGTTGATTTTGCTTGTGTTCAGCAACTGCTTCTACAAGTACTTGGGAGATCAGCAGCAGTGCTAAAACTGATGCTATTACTATGGTAAGCTTCATTTCAATTCTGATTCTTCTTCTTTCACAATCTTGTATGCATTTTGATGCCATTCTGTTGTGTATAAAAGGGCATATATAGACTGGTTTGTTTTGACCTTGCCTTGAAGTTTGTAATTATAATGTTGATTTCTATACCATTTGTGCTTGACTTCTTGAGTTTCAATTTCAAAGAGCAGAAGTCAGATAGCTTGAAGGATTTAAATGCAGCAGTGTGA

mRNA sequence

ATGGTCGCTTCCACATTCCATAAGCTATTGAGTTTTGGAGCAGTAACAGCAAGAAGATCCTCGAGTTTGCTACTGAAACTTTCTTCTAAGAATTTTATCGGAAGGATCCAAATGGGAACTAATCAAAACTCTATACTGAGAGGTTGCAGCAGTAACTCTAGAGGAGCCTTGGTGGTTCTTGAAGGTTTGGACCGTTGTGGGAAGACTACACAGTCTAGTAGATTAGTACAGTACTTAGAAGGACTTGGGCATTCTGCTGAACTATGGCGGTTTCCTGACAGAACTACAAGTGTTGGACAAATGATCTCTTCCTATCTTTCAAACAGATCACAACTGGATGACCACACAATACATCTTCTTTTCAGTGCCAATCGATGGGAGAAGAGATCGTTGATGGAAACTAAATTGAAGAGTGGAACCACTCTTATTGTTGATCGGTATTCTTATTCAGGGGTGGCCTTTTCATCTGCCAAAGGACTTGGCATTGAATGGTGTAAGGCACCGGAGGTTGGCTTGCTTGCACCGGACTTAGTACTGTACCTAGACATATCTCCAGAGAAAGCTGCAGAGAGAGGAGGATATGGATGTGAGAGATATGAGCAGCTTGAGTTCCAGAGCAAAGTTGCCCAGTACTATCAAGTTTTGCGTGATTCTTCATGGAAGCAGATTATTGATGCTTGCCAACCCATGGAAGATATTGAGAAACAACTTCAAGAGATTGTACTGGGCTGTGTCAAGAGATGCCACAGTGGTGTACCGCTTTTTCCCAGCTTTGGTGGCAGTGGCACCAATGGCTCATTGAAACCAGTATCTGGTGTTGGTGGTGTAGACACAAAGGGAAATGGTTCCTCTGGAATATCTGGAACTTCTGGAACCAGGGGAGGTGGAAGAAAGGTAATGGGAGGCTGTGGAATTGTGAAAGGCGTAGGAGGCGGCAGATCAGGCACTGGTGGAGGTAGTGAAAATTCTGGCAAAGGTGGTGAGAATGTTACTAAAGGTGTGGGAGTGGATAATAATGGTGGTGGTGAAGGAACCAGAGGTGGTGGAAACCAGGTTGAAGTAATGTTGGTGGTGGTGGTGAATGAACCAAGGGTGGTGGGGGAGATGAAGGAGGTGGAGATGGAGTTTCAGGTGAAGTCAAAGGCGGCGGTGGTGGCGATGTTGCAAGAAGGGGAGGCGGAGAAGGAACTAAGGGGGCTGGCTGATAAACTGGAGGAGGAGAAGAAACTAAAGGGGGCAGAGGAGGAAGTCTTTAGGGTGCCAAAAATGGGTTATCTGAAGGGGAGGTTGGAAAAGGCCATTGGCCACAGGCTCCAAATAAGTACTGCAACTGCTTCTACAAGTACTTGGGAGATCAGCAGCAGTGCTAAAACTGATGCTATTACTATGGGCATATATAGACTGGTTTGTTTTGACCTTGCCTTGAAGTTTGTAATTATAATGTTGATTTCTATACCATTTGTGCTTGACTTCTTGAGTTTCAATTTCAAAGAGCAGAAGTCAGATAGCTTGAAGGATTTAAATGCAGCAGTGTGA

Coding sequence (CDS)

ATGGTCGCTTCCACATTCCATAAGCTATTGAGTTTTGGAGCAGTAACAGCAAGAAGATCCTCGAGTTTGCTACTGAAACTTTCTTCTAAGAATTTTATCGGAAGGATCCAAATGGGAACTAATCAAAACTCTATACTGAGAGGTTGCAGCAGTAACTCTAGAGGAGCCTTGGTGGTTCTTGAAGGTTTGGACCGTTGTGGGAAGACTACACAGTCTAGTAGATTAGTACAGTACTTAGAAGGACTTGGGCATTCTGCTGAACTATGGCGGTTTCCTGACAGAACTACAAGTGTTGGACAAATGATCTCTTCCTATCTTTCAAACAGATCACAACTGGATGACCACACAATACATCTTCTTTTCAGTGCCAATCGATGGGAGAAGAGATCGTTGATGGAAACTAAATTGAAGAGTGGAACCACTCTTATTGTTGATCGGTATTCTTATTCAGGGGTGGCCTTTTCATCTGCCAAAGGACTTGGCATTGAATGGTGTAAGGCACCGGAGGTTGGCTTGCTTGCACCGGACTTAGTACTGTACCTAGACATATCTCCAGAGAAAGCTGCAGAGAGAGGAGGATATGGATGTGAGAGATATGAGCAGCTTGAGTTCCAGAGCAAAGTTGCCCAGTACTATCAAGTTTTGCGTGATTCTTCATGGAAGCAGATTATTGATGCTTGCCAACCCATGGAAGATATTGAGAAACAACTTCAAGAGATTGTACTGGGCTGTGTCAAGAGATGCCACAGTGGTGTACCGCTTTTTCCCAGCTTTGGTGGCAGTGGCACCAATGGCTCATTGAAACCAGTATCTGGTGTTGGTGGTGTAGACACAAAGGGAAATGGTTCCTCTGGAATATCTGGAACTTCTGGAACCAGGGGAGGTGGAAGAAAGGTAATGGGAGGCTGTGGAATTGTGAAAGGCGTAGGAGGCGGCAGATCAGGCACTGGTGGAGGTAGTGAAAATTCTGGCAAAGGTGGTGAGAATGTTACTAAAGGTGTGGGAGTGGATAATAATGGTGGTGGTGAAGGAACCAGAGGTGGTGGAAACCAGGTTGAAGTAATGTTGGTGGTGGTGGTGAATGAACCAAGGGTGGTGGGGGAGATGAAGGAGGTGGAGATGGAGTTTCAGGTGAAGTCAAAGGCGGCGGTGGTGGCGATGTTGCAAGAAGGGGAGGCGGAGAAGGAACTAAGGGGGCTGGCTGATAAACTGGAGGAGGAGAAGAAACTAAAGGGGGCAGAGGAGGAAGTCTTTAGGGTGCCAAAAATGGGTTATCTGAAGGGGAGGTTGGAAAAGGCCATTGGCCACAGGCTCCAAATAAGTACTGCAACTGCTTCTACAAGTACTTGGGAGATCAGCAGCAGTGCTAAAACTGATGCTATTACTATGGGCATATATAGACTGGTTTGTTTTGACCTTGCCTTGAAGTTTGTAATTATAATGTTGATTTCTATACCATTTGTGCTTGACTTCTTGAGTTTCAATTTCAAAGAGCAGAAGTCAGATAGCTTGAAGGATTTAAATGCAGCAGTGTGA

Protein sequence

MVASTFHKLLSFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEIVLGCVKRCHSGVPLFPSFGGSGTNGSLKPVSGVGGVDTKGNGSSGISGTSGTRGGGRKVMGGCGIVKGVGGGRSGTGGGSENSGKGGENVTKGVGVDNNGGGEGTRGGGNQVEVMLVVVVNEPRVVGEMKEVEMEFQVKSKAAVVAMLQEGEAEKELRGLADKLEEEKKLKGAEEEVFRVPKMGYLKGRLEKAIGHRLQISTATASTSTWEISSSAKTDAITMGIYRLVCFDLALKFVIIMLISIPFVLDFLSFNFKEQKSDSLKDLNAAV

Homology

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BLAST of Spg032675 vs. NCBI nr
Match: KAA0044460.1 (thymidylate kinase isoform X2 [Cucumis melo var. makuwa] >TYK29587.1 thymidylate kinase isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 477.2 bits (1227), Expect = 1.7e-130
Identity = 256/316 (81.01%), Postives = 273/316 (86.39%), Query Frame = 0


Query: 38  MGTNQNSILRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 97
           MGTNQNSI RG SSNSRGALVVLEGLDRCGKTTQSSRL QYLEGLGHS ELWRFPDRTTS
Sbjct: 1   MGTNQNSISRGSSSNSRGALVVLEGLDRCGKTTQSSRLTQYLEGLGHSVELWRFPDRTTS 60

Query: 98  VGQMISSYLSNRSQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSA 157
           VGQMISSYLSN SQLDDHT+HLLFSANRWEKRSLMETKLK GTTLI+DRYSYSGVAFS A
Sbjct: 61  VGQMISSYLSNESQLDDHTVHLLFSANRWEKRSLMETKLKGGTTLILDRYSYSGVAFSCA 120

Query: 158 KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRD 217
           KGL +EWCKAPEVGL APDLVLYL+ISPE AAERGGYG ERYE+++FQ+KV + YQVL  
Sbjct: 121 KGLDLEWCKAPEVGLPAPDLVLYLEISPEMAAERGGYGSERYERIDFQNKVIKSYQVLLG 180

Query: 218 SSWKQIIDACQPMEDIEKQLQEIVLGCVKRCHSGVPLFPSFGGSGTNGSLKPVSGVGGVD 277
           S+W QI+DAC+PME IEK+LQE VL C               GSGTNGSLKP+SG GG D
Sbjct: 181 SNW-QIVDACRPMEVIEKELQETVLDC---------------GSGTNGSLKPISGAGGAD 240

Query: 278 TKGNGSSGISGTSGTRGGGRKVMGGCGIVKGVGGGRSGTGGGSENSGKGGENVTKGVGVD 337
           TKGNGS GISGTSGTRGGGRKV+GGCG VKG GGGRS TGGGS+N G GGENVTKGVGVD
Sbjct: 241 TKGNGSCGISGTSGTRGGGRKVLGGCGNVKG-GGGRSSTGGGSKNCGIGGENVTKGVGVD 299

Query: 338 NNGGGEGTRGGGNQVE 354
           N+GGGE TRGGGNQVE
Sbjct: 301 NSGGGEETRGGGNQVE 299

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BLAST of Spg032675 vs. NCBI nr
Match: KAG6606822.1 (Thymidylate kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 456.8 bits (1174), Expect = 2.4e-124
Identity = 228/254 (89.76%), Postives = 242/254 (95.28%), Query Frame = 0


Query: 1   MVASTFHKLLSFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVL 60
           M+AST ++ LSF AV ARRSSS +LK+SSK FI  IQMGTNQNSIL+GC+SNSRGALVVL
Sbjct: 525 MLASTIYRPLSFEAVIARRSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVL 584

Query: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLL 120
           EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSN+SQLDDHTIHLL
Sbjct: 585 EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLL 644

Query: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY
Sbjct: 645 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 704

Query: 181 LDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEI 240
           L+ISPEKA+ERGGYGCERYEQLEFQSKVAQYYQVL DSSWK +IDACQPMEDIEKQLQE+
Sbjct: 705 LNISPEKASERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK-VIDACQPMEDIEKQLQEM 764

Query: 241 VLGCVKRCHSGVPL 255
           VL CVK+CH+G PL
Sbjct: 765 VLECVKKCHNGEPL 777

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BLAST of Spg032675 vs. NCBI nr
Match: XP_022998308.1 (thymidylate kinase isoform X1 [Cucurbita maxima])

HSP 1 Score: 455.7 bits (1171), Expect = 5.3e-124
Identity = 227/254 (89.37%), Postives = 242/254 (95.28%), Query Frame = 0


Query: 1   MVASTFHKLLSFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVL 60
           M+AST ++ LSF AV AR+SSS +LK+SSK FI  IQMGTNQNSIL+GC+SNSRGALVVL
Sbjct: 1   MLASTIYRPLSFEAVIARKSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVL 60

Query: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLL 120
           EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSN+SQLDDHTIHLL
Sbjct: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLL 120

Query: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY
Sbjct: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180

Query: 181 LDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEI 240
           L+ISPEKA+ERGGYGCERYEQLEFQSKVAQYYQVL DSSWK +IDACQPMEDIEKQLQE+
Sbjct: 181 LNISPEKASERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK-VIDACQPMEDIEKQLQEM 240

Query: 241 VLGCVKRCHSGVPL 255
           VL CVK+CH+G PL
Sbjct: 241 VLECVKKCHNGEPL 253

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BLAST of Spg032675 vs. NCBI nr
Match: XP_022948490.1 (thymidylate kinase isoform X1 [Cucurbita moschata])

HSP 1 Score: 454.5 bits (1168), Expect = 1.2e-123
Identity = 227/254 (89.37%), Postives = 241/254 (94.88%), Query Frame = 0


Query: 1   MVASTFHKLLSFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVL 60
           M+AST ++ LSF AV ARRSSS +LK+SSK FI  IQMGTNQNSIL+GC+SNSRGALVVL
Sbjct: 1   MLASTIYRPLSFEAVIARRSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVL 60

Query: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLL 120
           EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSN+SQLDDHTIHLL
Sbjct: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLL 120

Query: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRS METKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY
Sbjct: 121 FSANRWEKRSSMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180

Query: 181 LDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEI 240
           L+ISPEKA+ERGGYGCERYEQLEFQSKVAQYYQVL DSSWK +IDACQPMEDIEKQLQE+
Sbjct: 181 LNISPEKASERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK-VIDACQPMEDIEKQLQEM 240

Query: 241 VLGCVKRCHSGVPL 255
           VL CVK+CH+G PL
Sbjct: 241 VLECVKKCHNGEPL 253

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BLAST of Spg032675 vs. NCBI nr
Match: XP_023523991.1 (thymidylate kinase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 454.1 bits (1167), Expect = 1.6e-123
Identity = 226/254 (88.98%), Postives = 241/254 (94.88%), Query Frame = 0


Query: 1   MVASTFHKLLSFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVL 60
           M+AST ++ LSF AV ARRSSS +LK+SSK FI  IQMGTNQNSIL+GC+SNSRGALVVL
Sbjct: 1   MLASTIYRPLSFEAVIARRSSSFILKISSKAFIRNIQMGTNQNSILQGCNSNSRGALVVL 60

Query: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLL 120
           EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSN+SQLDDHTIHLL
Sbjct: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLL 120

Query: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY
Sbjct: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180

Query: 181 LDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEI 240
           L+ISPEKA+ERGGYGCERYEQLEFQSKV QYYQVL DSSWK +IDACQPMEDIEKQLQE+
Sbjct: 181 LNISPEKASERGGYGCERYEQLEFQSKVDQYYQVLHDSSWK-VIDACQPMEDIEKQLQEM 240

Query: 241 VLGCVKRCHSGVPL 255
           VL CVK+CH+G P+
Sbjct: 241 VLECVKKCHNGEPI 253

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BLAST of Spg032675 vs. ExPASy Swiss-Prot
Match: Q0WW55 (Thymidylate kinase OS=Arabidopsis thaliana OX=3702 GN=ZEU1 PE=2 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 4.2e-87
Identity = 163/244 (66.80%), Postives = 195/244 (79.92%), Query Frame = 0


Query: 11  SFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVLEGLDRCGKTT 70
           SF A+ + RS +  L+   ++ + R++     + +    S   RGAL+VLEGLDR GK+T
Sbjct: 16  SFRALASPRSLNYPLQCIKRSSV-RMESSNFSSGVRTESSVKPRGALIVLEGLDRSGKST 75

Query: 71  QSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLLFSANRWEKRS 130
           Q ++L+ +L GLGH  ELWRFPDR TSVGQMIS+YLSN+SQLDDHTIHLLFSANRWEKRS
Sbjct: 76  QCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLDDHTIHLLFSANRWEKRS 135

Query: 131 LMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAAE 190
           LME KLK+GTTLIVDRYSYSGVAFSSAKGL IEWCKAPE+GLLAPD VLYLDISPE+AAE
Sbjct: 136 LMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPEIGLLAPDSVLYLDISPERAAE 195

Query: 191 RGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEIVLGCVKRCHS 250
           RGGYG ERYE++EFQ KVA +YQ L DSSWK II+A   ME++EK++Q++VL  VK C  
Sbjct: 196 RGGYGDERYERVEFQKKVADFYQTLGDSSWK-IINASDAMEEVEKKIQQVVLDQVKECTE 255

Query: 251 GVPL 255
           G PL
Sbjct: 256 GKPL 257

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BLAST of Spg032675 vs. ExPASy Swiss-Prot
Match: P23919 (Thymidylate kinase OS=Homo sapiens OX=9606 GN=DTYMK PE=1 SV=4)

HSP 1 Score: 196.8 bits (499), Expect = 5.9e-49
Identity = 94/189 (49.74%), Postives = 141/189 (74.60%), Query Frame = 0


Query: 54  RGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLD 113
           RGAL+VLEG+DR GK+TQS +LV+ L   GH AEL RFP+R+T +G+++SSYL  +S ++
Sbjct: 5   RGALIVLEGVDRAGKSTQSRKLVEALCAAGHRAELLRFPERSTEIGKLLSSYLQKKSDVE 64

Query: 114 DHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAK-GLGIEWCKAPEVGL 173
           DH++HLLFSANRWE+  L++ KL  G TL+VDRY++SGVAF+ AK    ++WCK P+VGL
Sbjct: 65  DHSVHLLFSANRWEQVPLIKEKLSQGVTLVVDRYAFSGVAFTGAKENFSLDWCKQPDVGL 124

Query: 174 LAPDLVLYLDISPEKAAERGGYGCERYEQLEFQSKVAQ-YYQVLRDS--SWKQIIDACQP 233
             PDLVL+L +    AA+RG +G ERYE   FQ +  + ++Q+++D+  +WK ++DA + 
Sbjct: 125 PKPDLVLFLQLQLADAAKRGAFGHERYENGAFQERALRCFHQLMKDTTLNWK-MVDASKS 184

Query: 234 MEDIEKQLQ 239
           +E + + ++
Sbjct: 185 IEAVHEDIR 192

[Close]

BLAST of Spg032675 vs. ExPASy Swiss-Prot
Match: P97930 (Thymidylate kinase OS=Mus musculus OX=10090 GN=Dtymk PE=1 SV=2)

HSP 1 Score: 195.7 bits (496), Expect = 1.3e-48
Identity = 93/192 (48.44%), Postives = 140/192 (72.92%), Query Frame = 0


Query: 51  SNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRS 110
           ++ RGAL+VLEG+DR GKTTQ  +LV  L   GH AEL RFP+R+T +G++++SYL  ++
Sbjct: 2   ASRRGALIVLEGVDRAGKTTQGLKLVTALCASGHRAELLRFPERSTEIGKLLNSYLEKKT 61

Query: 111 QLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAK-GLGIEWCKAPE 170
           +L+DH++HLLFSANRWE+  L++ KL  G TL++DRY++SGVAF+ AK    ++WCK P+
Sbjct: 62  ELEDHSVHLLFSANRWEQVPLIKAKLNQGVTLVLDRYAFSGVAFTGAKENFSLDWCKQPD 121

Query: 171 VGLLAPDLVLYLDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVL---RDSSWKQIIDA 230
           VGL  PDL+L+L +    AA RG +G ERYE   FQ +V   +Q L   ++ +WK ++DA
Sbjct: 122 VGLPKPDLILFLQLQLLDAAARGEFGLERYETGTFQKQVLLCFQQLMEEKNLNWK-VVDA 181

Query: 231 CQPMEDIEKQLQ 239
            + +E++ K+++
Sbjct: 182 SKSIEEVHKEIR 192

[Close]

BLAST of Spg032675 vs. ExPASy Swiss-Prot
Match: P36590 (Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tmp1 PE=2 SV=2)

HSP 1 Score: 189.9 bits (481), Expect = 7.2e-47
Identity = 94/197 (47.72%), Postives = 131/197 (66.50%), Query Frame = 0


Query: 50  SSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNR 109
           S  +RG L+V+EGLDR GK+TQ   LV  L      AEL++FPDRTT++G+ I  YL   
Sbjct: 2   SKQNRGRLIVIEGLDRSGKSTQCQLLVDKLISQHEKAELFKFPDRTTAIGKKIDDYLKES 61

Query: 110 SQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPE 169
            QL+D  IHLLFSANRWE    +  ++  G T I+DRY++SG+AFS+AKGL  EWCK+P+
Sbjct: 62  VQLNDQVIHLLFSANRWETIQYIYEQINKGVTCILDRYAFSGIAFSAAKGLDWEWCKSPD 121

Query: 170 VGLLAPDLVLYLDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQ-----II 229
            GL  PDLV++L++ P  AA RG YG ERYE++E Q KV + +Q L+    ++      +
Sbjct: 122 RGLPRPDLVIFLNVDPRIAATRGQYGEERYEKIEMQEKVLKNFQRLQKEFREEGLEFITL 181

Query: 230 DACQPMEDIEKQLQEIV 242
           DA   +ED+  Q+ ++V
Sbjct: 182 DASSSLEDVHSQIVDLV 198

[Close]

BLAST of Spg032675 vs. ExPASy Swiss-Prot
Match: Q54GN2 (Thymidylate kinase OS=Dictyostelium discoideum OX=44689 GN=dtymk PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.8e-45
Identity = 91/189 (48.15%), Postives = 127/189 (67.20%), Query Frame = 0


Query: 54  RGALVVLEGLDRCGKTTQSSRLVQYLEGLGH-SAELWRFPDRTTSVGQMISSYLSNRSQL 113
           RG  ++ EG+DR GK+TQ   L  ++  +     +  RFPDRTT +GQ+I+ YL N + +
Sbjct: 18  RGLFILFEGVDRVGKSTQVQSLTNHISNVQKLPTKSLRFPDRTTPIGQIINQYLQNATNM 77

Query: 114 DDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGL 173
           DD  +HLLFS+NRWE R  +   L +GT ++VDRYSYSGVA+S+AKG+  +WC A E GL
Sbjct: 78  DDRALHLLFSSNRWEARDSILELLNNGTNIVVDRYSYSGVAYSAAKGIDFDWCYACEKGL 137

Query: 174 LAPDLVLYLDISPEKAAERGGYGCERYEQLEFQSKVAQYY-QVLRDSSWKQIIDACQPME 233
             PDL+ YL +S E A +RG YG ERYE+LEFQ K+ Q Y + L D  WK II+A + ++
Sbjct: 138 PKPDLIFYLSMSSEDATKRGEYGGERYEKLEFQKKIKQIYEEKLVDDQWK-IINANRSID 197

Query: 234 DIEKQLQEI 241
           +I  ++  I
Sbjct: 198 EISNEISSI 205

[Close]

BLAST of Spg032675 vs. ExPASy TrEMBL
Match: A0A5D3E0G4 (dTMP kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001950 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 8.3e-131
Identity = 256/316 (81.01%), Postives = 273/316 (86.39%), Query Frame = 0


Query: 38  MGTNQNSILRGCSSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTS 97
           MGTNQNSI RG SSNSRGALVVLEGLDRCGKTTQSSRL QYLEGLGHS ELWRFPDRTTS
Sbjct: 1   MGTNQNSISRGSSSNSRGALVVLEGLDRCGKTTQSSRLTQYLEGLGHSVELWRFPDRTTS 60

Query: 98  VGQMISSYLSNRSQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSA 157
           VGQMISSYLSN SQLDDHT+HLLFSANRWEKRSLMETKLK GTTLI+DRYSYSGVAFS A
Sbjct: 61  VGQMISSYLSNESQLDDHTVHLLFSANRWEKRSLMETKLKGGTTLILDRYSYSGVAFSCA 120

Query: 158 KGLGIEWCKAPEVGLLAPDLVLYLDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRD 217
           KGL +EWCKAPEVGL APDLVLYL+ISPE AAERGGYG ERYE+++FQ+KV + YQVL  
Sbjct: 121 KGLDLEWCKAPEVGLPAPDLVLYLEISPEMAAERGGYGSERYERIDFQNKVIKSYQVLLG 180

Query: 218 SSWKQIIDACQPMEDIEKQLQEIVLGCVKRCHSGVPLFPSFGGSGTNGSLKPVSGVGGVD 277
           S+W QI+DAC+PME IEK+LQE VL C               GSGTNGSLKP+SG GG D
Sbjct: 181 SNW-QIVDACRPMEVIEKELQETVLDC---------------GSGTNGSLKPISGAGGAD 240

Query: 278 TKGNGSSGISGTSGTRGGGRKVMGGCGIVKGVGGGRSGTGGGSENSGKGGENVTKGVGVD 337
           TKGNGS GISGTSGTRGGGRKV+GGCG VKG GGGRS TGGGS+N G GGENVTKGVGVD
Sbjct: 241 TKGNGSCGISGTSGTRGGGRKVLGGCGNVKG-GGGRSSTGGGSKNCGIGGENVTKGVGVD 299

Query: 338 NNGGGEGTRGGGNQVE 354
           N+GGGE TRGGGNQVE
Sbjct: 301 NSGGGEETRGGGNQVE 299

[Close]

BLAST of Spg032675 vs. ExPASy TrEMBL
Match: A0A6J1KGD6 (dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 2.6e-124
Identity = 227/254 (89.37%), Postives = 242/254 (95.28%), Query Frame = 0


Query: 1   MVASTFHKLLSFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVL 60
           M+AST ++ LSF AV AR+SSS +LK+SSK FI  IQMGTNQNSIL+GC+SNSRGALVVL
Sbjct: 1   MLASTIYRPLSFEAVIARKSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVL 60

Query: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLL 120
           EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSN+SQLDDHTIHLL
Sbjct: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLL 120

Query: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY
Sbjct: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180

Query: 181 LDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEI 240
           L+ISPEKA+ERGGYGCERYEQLEFQSKVAQYYQVL DSSWK +IDACQPMEDIEKQLQE+
Sbjct: 181 LNISPEKASERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK-VIDACQPMEDIEKQLQEM 240

Query: 241 VLGCVKRCHSGVPL 255
           VL CVK+CH+G PL
Sbjct: 241 VLECVKKCHNGEPL 253

[Close]

BLAST of Spg032675 vs. ExPASy TrEMBL
Match: A0A6J1G9Z9 (dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 5.8e-124
Identity = 227/254 (89.37%), Postives = 241/254 (94.88%), Query Frame = 0


Query: 1   MVASTFHKLLSFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVL 60
           M+AST ++ LSF AV ARRSSS +LK+SSK FI  IQMGTNQNSIL+GC+SNSRGALVVL
Sbjct: 1   MLASTIYRPLSFEAVIARRSSSFILKVSSKAFIRNIQMGTNQNSILQGCNSNSRGALVVL 60

Query: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLL 120
           EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSN+SQLDDHTIHLL
Sbjct: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNKSQLDDHTIHLL 120

Query: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRS METKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY
Sbjct: 121 FSANRWEKRSSMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180

Query: 181 LDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEI 240
           L+ISPEKA+ERGGYGCERYEQLEFQSKVAQYYQVL DSSWK +IDACQPMEDIEKQLQE+
Sbjct: 181 LNISPEKASERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK-VIDACQPMEDIEKQLQEM 240

Query: 241 VLGCVKRCHSGVPL 255
           VL CVK+CH+G PL
Sbjct: 241 VLECVKKCHNGEPL 253

[Close]

BLAST of Spg032675 vs. ExPASy TrEMBL
Match: A0A6J1DIU8 (dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 1.4e-122
Identity = 229/249 (91.97%), Postives = 234/249 (93.98%), Query Frame = 0


Query: 6   FHKLLSFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVLEGLDR 65
           FH  LSFGAVTARRSSS LLK+ S N I +IQMGTN NS+LRGCSSNSRGALVVLEGLDR
Sbjct: 5   FH--LSFGAVTARRSSSALLKIPSNNLIRKIQMGTNHNSVLRGCSSNSRGALVVLEGLDR 64

Query: 66  CGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLLFSANR 125
           CGKTTQSSRLVQYLEGLGHSAEL RFPDRTTSVGQMISSYLSN SQLDDHTIHLLFSANR
Sbjct: 65  CGKTTQSSRLVQYLEGLGHSAELLRFPDRTTSVGQMISSYLSNESQLDDHTIHLLFSANR 124

Query: 126 WEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLDISP 185
           WEKRSLMETKLKSGTTLIVDRYSYSGVAFS+AKGLGIEWCKAPEVGLLAPDLVLYLDISP
Sbjct: 125 WEKRSLMETKLKSGTTLIVDRYSYSGVAFSAAKGLGIEWCKAPEVGLLAPDLVLYLDISP 184

Query: 186 EKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEIVLGCV 245
           EKA ERGGYGCERYEQLEFQSKVAQYYQVL DSSWK IIDACQPMEDIEKQLQEIVLGCV
Sbjct: 185 EKAVERGGYGCERYEQLEFQSKVAQYYQVLHDSSWK-IIDACQPMEDIEKQLQEIVLGCV 244

Query: 246 KRCHSGVPL 255
           KR H+G PL
Sbjct: 245 KRRHNGEPL 250

[Close]

BLAST of Spg032675 vs. ExPASy TrEMBL
Match: A0A6J1DDX5 (dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111019882 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 2.5e-119
Identity = 225/254 (88.58%), Postives = 233/254 (91.73%), Query Frame = 0


Query: 1   MVASTFHKLLSFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVL 60
           M+A TFHK LSF AVTARRSSS  LK+ SKN I +IQMGTNQNS+LRGCSSNSRGALVVL
Sbjct: 1   MLAFTFHKPLSFRAVTARRSSSSPLKIPSKNSIQKIQMGTNQNSVLRGCSSNSRGALVVL 60

Query: 61  EGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLL 120
           EGLDRCGKTTQSSRLVQYLEGLG SAELWRFPDRTTSVGQMISSYLSN+SQLDD TIHLL
Sbjct: 61  EGLDRCGKTTQSSRLVQYLEGLGFSAELWRFPDRTTSVGQMISSYLSNKSQLDDRTIHLL 120

Query: 121 FSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLY 180
           FSANRWEKRSLME+KLKSGTTLIVDRYSYSGVAFS+AKGL IEWCKAPEVGLLA D VLY
Sbjct: 121 FSANRWEKRSLMESKLKSGTTLIVDRYSYSGVAFSAAKGLDIEWCKAPEVGLLASDSVLY 180

Query: 181 LDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEI 240
           LDISPE AAERGGYG ERYEQLEFQ KV QYYQVL DSSWK IIDACQ MEDIEKQLQEI
Sbjct: 181 LDISPENAAERGGYGRERYEQLEFQRKVYQYYQVLHDSSWK-IIDACQTMEDIEKQLQEI 240

Query: 241 VLGCVKRCHSGVPL 255
           VLGCVKRCH+G PL
Sbjct: 241 VLGCVKRCHNGEPL 253

[Close]

BLAST of Spg032675 vs. TAIR 10
Match: AT5G59440.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 323.6 bits (828), Expect = 3.0e-88
Identity = 163/244 (66.80%), Postives = 195/244 (79.92%), Query Frame = 0


Query: 11  SFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVLEGLDRCGKTT 70
           SF A+ + RS +  L+   ++ + R++     + +    S   RGAL+VLEGLDR GK+T
Sbjct: 16  SFRALASPRSLNYPLQCIKRSSV-RMESSNFSSGVRTESSVKPRGALIVLEGLDRSGKST 75

Query: 71  QSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLLFSANRWEKRS 130
           Q ++L+ +L GLGH  ELWRFPDR TSVGQMIS+YLSN+SQLDDHTIHLLFSANRWEKRS
Sbjct: 76  QCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLDDHTIHLLFSANRWEKRS 135

Query: 131 LMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAAE 190
           LME KLK+GTTLIVDRYSYSGVAFSSAKGL IEWCKAPE+GLLAPD VLYLDISPE+AAE
Sbjct: 136 LMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPEIGLLAPDSVLYLDISPERAAE 195

Query: 191 RGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEIVLGCVKRCHS 250
           RGGYG ERYE++EFQ KVA +YQ L DSSWK II+A   ME++EK++Q++VL  VK C  
Sbjct: 196 RGGYGDERYERVEFQKKVADFYQTLGDSSWK-IINASDAMEEVEKKIQQVVLDQVKECTE 255

Query: 251 GVPL 255
           G PL
Sbjct: 256 GKPL 257

[Close]

BLAST of Spg032675 vs. TAIR 10
Match: AT5G59440.3 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 323.6 bits (828), Expect = 3.0e-88
Identity = 163/244 (66.80%), Postives = 195/244 (79.92%), Query Frame = 0


Query: 11  SFGAVTARRSSSLLLKLSSKNFIGRIQMGTNQNSILRGCSSNSRGALVVLEGLDRCGKTT 70
           SF A+ + RS +  L+   ++ + R++     + +    S   RGAL+VLEGLDR GK+T
Sbjct: 24  SFRALASPRSLNYPLQCIKRSSV-RMESSNFSSGVRTESSVKPRGALIVLEGLDRSGKST 83

Query: 71  QSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNRSQLDDHTIHLLFSANRWEKRS 130
           Q ++L+ +L GLGH  ELWRFPDR TSVGQMIS+YLSN+SQLDDHTIHLLFSANRWEKRS
Sbjct: 84  QCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNKSQLDDHTIHLLFSANRWEKRS 143

Query: 131 LMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPEVGLLAPDLVLYLDISPEKAAE 190
           LME KLK+GTTLIVDRYSYSGVAFSSAKGL IEWCKAPE+GLLAPD VLYLDISPE+AAE
Sbjct: 144 LMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPEIGLLAPDSVLYLDISPERAAE 203

Query: 191 RGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQPMEDIEKQLQEIVLGCVKRCHS 250
           RGGYG ERYE++EFQ KVA +YQ L DSSWK II+A   ME++EK++Q++VL  VK C  
Sbjct: 204 RGGYGDERYERVEFQKKVADFYQTLGDSSWK-IINASDAMEEVEKKIQQVVLDQVKECTE 263

Query: 251 GVPL 255
           G PL
Sbjct: 264 GKPL 265

[Close]

BLAST of Spg032675 vs. TAIR 10
Match: AT5G59440.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 320.5 bits (820), Expect = 2.5e-87
Identity = 156/205 (76.10%), Postives = 177/205 (86.34%), Query Frame = 0


Query: 50  SSNSRGALVVLEGLDRCGKTTQSSRLVQYLEGLGHSAELWRFPDRTTSVGQMISSYLSNR 109
           S   RGAL+VLEGLDR GK+TQ ++L+ +L GLGH  ELWRFPDR TSVGQMIS+YLSN+
Sbjct: 15  SVKPRGALIVLEGLDRSGKSTQCAKLLSFLNGLGHPTELWRFPDRETSVGQMISAYLSNK 74

Query: 110 SQLDDHTIHLLFSANRWEKRSLMETKLKSGTTLIVDRYSYSGVAFSSAKGLGIEWCKAPE 169
           SQLDDHTIHLLFSANRWEKRSLME KLK+GTTLIVDRYSYSGVAFSSAKGL IEWCKAPE
Sbjct: 75  SQLDDHTIHLLFSANRWEKRSLMEEKLKTGTTLIVDRYSYSGVAFSSAKGLDIEWCKAPE 134

Query: 170 VGLLAPDLVLYLDISPEKAAERGGYGCERYEQLEFQSKVAQYYQVLRDSSWKQIIDACQP 229
           +GLLAPD VLYLDISPE+AAERGGYG ERYE++EFQ KVA +YQ L DSSWK II+A   
Sbjct: 135 IGLLAPDSVLYLDISPERAAERGGYGDERYERVEFQKKVADFYQTLGDSSWK-IINASDA 194

Query: 230 MEDIEKQLQEIVLGCVKRCHSGVPL 255
           ME++EK++Q++VL  VK C  G PL
Sbjct: 195 MEEVEKKIQQVVLDQVKECTEGKPL 218

The following BLAST results are available for this feature:

BLAST of Spg032675 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0044460.1	1.7e-130	81.01	thymidylate kinase isoform X2 [Cucumis melo var. makuwa] >TYK29587.1 thymidylate...	[more]
KAG6606822.1	2.4e-124	89.76	Thymidylate kinase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022998308.1	5.3e-124	89.37	thymidylate kinase isoform X1 [Cucurbita maxima]	[more]
XP_022948490.1	1.2e-123	89.37	thymidylate kinase isoform X1 [Cucurbita moschata]	[more]
XP_023523991.1	1.6e-123	88.98	thymidylate kinase isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Spg032675 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WW55	4.2e-87	66.80	Thymidylate kinase OS=Arabidopsis thaliana OX=3702 GN=ZEU1 PE=2 SV=1	[more]
P23919	5.9e-49	49.74	Thymidylate kinase OS=Homo sapiens OX=9606 GN=DTYMK PE=1 SV=4	[more]
P97930	1.3e-48	48.44	Thymidylate kinase OS=Mus musculus OX=10090 GN=Dtymk PE=1 SV=2	[more]
P36590	7.2e-47	47.72	Thymidylate kinase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284...	[more]
Q54GN2	1.8e-45	48.15	Thymidylate kinase OS=Dictyostelium discoideum OX=44689 GN=dtymk PE=3 SV=1	[more]

BLAST of Spg032675 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3E0G4	8.3e-131	81.01	dTMP kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001950 P...	[more]
A0A6J1KGD6	2.6e-124	89.37	dTMP kinase OS=Cucurbita maxima OX=3661 GN=LOC111492984 PE=3 SV=1	[more]
A0A6J1G9Z9	5.8e-124	89.37	dTMP kinase OS=Cucurbita moschata OX=3662 GN=LOC111452153 PE=3 SV=1	[more]
A0A6J1DIU8	1.4e-122	91.97	dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111020933 PE=3 SV=1	[more]
A0A6J1DDX5	2.5e-119	88.58	dTMP kinase OS=Momordica charantia OX=3673 GN=LOC111019882 PE=3 SV=1	[more]

BLAST of Spg032675 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59440.1	3.0e-88	66.80	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT5G59440.3	3.0e-88	66.80	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]
AT5G59440.2	2.5e-87	76.10	P-loop containing nucleoside triphosphate hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 390..410
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 277..291
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 275..295
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 310..348
None	No IPR available	PANTHER	PTHR10344	THYMIDYLATE KINASE	coord: 52..251
None	No IPR available	PANTHER	PTHR10344:SF1	THYMIDYLATE KINASE	coord: 52..251
None	No IPR available	CDD	cd01672	TMPK	coord: 57..242 e-value: 1.10646E-26 score: 105.043
IPR039430	Thymidylate kinase-like domain	PFAM	PF02223	Thymidylate_kin	coord: 60..236 e-value: 1.2E-43 score: 148.9
IPR018094	Thymidylate kinase	TIGRFAM	TIGR00041	TIGR00041	coord: 54..236 e-value: 1.4E-36 score: 124.1
IPR018094	Thymidylate kinase	HAMAP	MF_00165	Thymidylate_kinase	coord: 56..245 score: 15.855136
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 49..253 e-value: 2.5E-65 score: 221.9
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 54..248
IPR018095	Thymidylate kinase, conserved site	PROSITE	PS01331	THYMIDYLATE_KINASE	coord: 143..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg032675.1	Spg032675.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006233	dTDP biosynthetic process
biological_process	GO:0046940	nucleoside monophosphate phosphorylation
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004798	thymidylate kinase activity