Spg032591 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg032591 vs. NCBI nr
Match: XP_004147287.1 (heavy metal-associated isoprenylated plant protein 3 [Cucumis sativus] >KGN64800.1 hypothetical protein Csa_013474 [Cucumis sativus])

HSP 1 Score: 468.4 bits (1204), Expect = 5.4e-128
Identity = 275/348 (79.02%), Postives = 295/348 (84.77%), Query Frame = 0

Query: 1   MGE-AVPKKKKNENEKNGDGGGG-AKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGV 60
           MGE AV KKKKN+NEKNGDGGGG  KKKEE P T+VLK+DMHCEGCANKI KCVKGFEGV
Sbjct: 1   MGEAAVQKKKKNDNEKNGDGGGGEGKKKEEIPFTIVLKIDMHCEGCANKITKCVKGFEGV 60

Query: 61  QSVKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQT 120
           QSVK EI+ NKLTVMGKKIDA+KLREKLSNKTKKKVDLISPQPKKEKDSKPK+  D+DQT
Sbjct: 61  QSVKAEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQT 120

Query: 121 STNNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQK 180
           S+NNNKSDKKTDENKKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGVQEMSVD+QK
Sbjct: 121 SSNNNKSDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDKQK 180

Query: 181 DLVTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSGDKKDIPAGDVEK 240
           D V VKGTMDVKAL  SL+ERLKRTVEIVP KKEK+K+ +NN K   G       GD +K
Sbjct: 181 DSVMVKGTMDVKALIGSLSERLKRTVEIVPAKKEKEKEKDNNKKEGGG------GGDEKK 240

Query: 241 NNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEE-CGAGAKMEGNKMEYMGMGGIG 300
           ++T G G+GNGNGGGKKKKKGGNG       GGGDGEE  G G KMEGNKMEYMGMGGIG
Sbjct: 241 DSTTGDGDGNGNGGGKKKKKGGNG-------GGGDGEEGGGGGGKMEGNKMEYMGMGGIG 300

Query: 301 YGYPYGLSNGYGYGVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
           YGY Y    GYGYG+  GYGYGP G+VGENLHAPQLFSDENPNAC IM
Sbjct: 301 YGYGY----GYGYGMNTGYGYGPSGIVGENLHAPQLFSDENPNACFIM 331

BLAST of Spg032591 vs. NCBI nr
Match: XP_008463119.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo] >KAA0051833.1 heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 466.8 bits (1200), Expect = 1.6e-127
Identity = 275/348 (79.02%), Postives = 291/348 (83.62%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGEAV KKKKN+NEKNGDG GG KKKE+ P T+VLK+DMHCEGCANKI KCVKGFEGVQS
Sbjct: 1   MGEAVQKKKKNDNEKNGDGEGGGKKKEDIPFTIVLKIDMHCEGCANKITKCVKGFEGVQS 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTST 120
           VK EI+ NKLTVMGKKIDA+KLREKLSNKTKKKVDLISPQPKKEKDSKPK+  D+DQTS+
Sbjct: 61  VKAEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSS 120

Query: 121 NNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDL 180
           NNNK DKKTDENKKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGVQEMSVDRQKD 
Sbjct: 121 NNNKFDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDRQKDS 180

Query: 181 VTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSG---DKKDIPAGDVE 240
           V VKGTMDVKAL  SL+ERLKR VEIVP KKEK+K +  NNKN+ G   DKKD P GD  
Sbjct: 181 VMVKGTMDVKALIGSLSERLKRPVEIVPAKKEKEK-EKENNKNEGGGGDDKKDSPTGD-- 240

Query: 241 KNNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGMGGIG 300
               +G GNGNGNGGGKKKKKGGNG G     GGG     G G KMEGNKMEYMGMGGIG
Sbjct: 241 ---GDGNGNGNGNGGGKKKKKGGNGGGGEGEEGGG-----GGGGKMEGNKMEYMGMGGIG 300

Query: 301 YGYPYGLSNGYGYGVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
           YGY      GYGYG+  GYGYGPGGLVGENLHAPQLFSDENPNAC IM
Sbjct: 301 YGY------GYGYGLNTGYGYGPGGLVGENLHAPQLFSDENPNACFIM 331

BLAST of Spg032591 vs. NCBI nr
Match: XP_038894057.1 (heavy metal-associated isoprenylated plant protein 3-like [Benincasa hispida])

HSP 1 Score: 463.4 bits (1191), Expect = 1.7e-126
Identity = 274/345 (79.42%), Postives = 286/345 (82.90%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGEAV KKKKNENE NGDGGGG KKKEE P TVVLK+DMHCEGCANKI KCVKGFEGVQS
Sbjct: 1   MGEAVQKKKKNENENNGDGGGGGKKKEEIPFTVVLKIDMHCEGCANKITKCVKGFEGVQS 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTST 120
           VK EI+ NKLTV GKKIDA+KLREKLSNKTKKKVDLISPQPKKEKDSKPK+  D+DQTS+
Sbjct: 61  VKAEIDGNKLTVTGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSS 120

Query: 121 NNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDL 180
           NNNKSDKKTDENKKK KEPPVTTAVLKV+LHCQGCIEKI RVT+KFKGVQEMSVDRQKDL
Sbjct: 121 NNNKSDKKTDENKKKSKEPPVTTAVLKVSLHCQGCIEKIQRVTTKFKGVQEMSVDRQKDL 180

Query: 181 VTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSGDKKDIPAGDVEKNN 240
           VTVKGTMDVKALS SLTERLKR VEIVPMKKEKD     NNKN+ G       GD +   
Sbjct: 181 VTVKGTMDVKALSGSLTERLKRPVEIVPMKKEKD-----NNKNEGGG----GGGDKKDPA 240

Query: 241 TEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGMGGIGYGY 300
             G GNGNGNGGGKKKKKGGNG   G G G G+G E G G KMEGNKMEYMGM GIGYG+
Sbjct: 241 ATGDGNGNGNGGGKKKKKGGNG---GGGGGDGNGGEDGCGGKMEGNKMEYMGMVGIGYGH 300

Query: 301 PYGLSNGYGYGVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
                       GYGYGYGPGGLVGENLHAPQLFSDENPNAC IM
Sbjct: 301 ------------GYGYGYGPGGLVGENLHAPQLFSDENPNACFIM 321

BLAST of Spg032591 vs. NCBI nr
Match: KAG6583560.1 (Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019268.1 Heavy metal-associated isoprenylated plant protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 454.1 bits (1167), Expect = 1.0e-123
Identity = 282/355 (79.44%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGE   KK KNENEKNGDGGGG KKKEENP TVVLKVDMHCEGCANKI KCVKGFEGVQ+
Sbjct: 1   MGE---KKTKNENEKNGDGGGG-KKKEENPFTVVLKVDMHCEGCANKITKCVKGFEGVQT 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTST 120
           VK EIE NKLTV G+KIDASKL EKLSNKTKKKVDLISPQ KKEKDSKPK   DEDQTS+
Sbjct: 61  VKAEIEGNKLTVTGEKIDASKLLEKLSNKTKKKVDLISPQSKKEKDSKPKVKGDEDQTSS 120

Query: 121 NNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDL 180
           NNNKSDKKT+ENKKKPKEPPVTTAVLKVALHCQGCIEKI R+T+KFKGVQEMS+D+QK+L
Sbjct: 121 NNNKSDKKTEENKKKPKEPPVTTAVLKVALHCQGCIEKIQRITTKFKGVQEMSLDKQKEL 180

Query: 181 VTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDD---NNNNKND--SGDKKDIPAGD 240
           VTVKGTMDVKALS  LTERLKR VEIVP KKEKDKD+   NNNNKN+   G+KK+ PA  
Sbjct: 181 VTVKGTMDVKALSGCLTERLKRAVEIVPTKKEKDKDNNNTNNNNKNEGGGGNKKEPPA-- 240

Query: 241 VEKNNTEGGGNGNGNGGGKKKKKGGNGNGNG--NGNGGGDG-EECGAGAKMEGNKMEYMG 300
                 EG GN N NGGGKKKKKGGNGNG G   G+GG  G EE G G KMEG KMEYMG
Sbjct: 241 ----TGEGDGNSNENGGGKKKKKGGNGNGGGGSGGDGGATGMEEGGGGGKMEGYKMEYMG 300

Query: 301 MGGIGYGYPYGLSNGYGY--GVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
           MGG G GY YGL NGYG   G+GYGYGYG GG+VGENLHAPQLFSDENPNACSIM
Sbjct: 301 MGG-GIGYGYGL-NGYGMSTGLGYGYGYGAGGVVGENLHAPQLFSDENPNACSIM 343

BLAST of Spg032591 vs. NCBI nr
Match: XP_022965017.1 (heavy metal-associated isoprenylated plant protein 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 447.2 bits (1149), Expect = 1.3e-121
Identity = 275/351 (78.35%), Postives = 291/351 (82.91%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGE   KK KNENEKNGDGGGG KKKEENP TVVLKVDMHCEGCANKI KCVKGFEGVQ+
Sbjct: 1   MGE---KKTKNENEKNGDGGGG-KKKEENPFTVVLKVDMHCEGCANKITKCVKGFEGVQT 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTST 120
           VK EIE NKLTV G+KIDASKL EKLSNKTKKKVDLISPQ KKEKDSKPK   DEDQTS+
Sbjct: 61  VKAEIEGNKLTVTGEKIDASKLLEKLSNKTKKKVDLISPQSKKEKDSKPKVKGDEDQTSS 120

Query: 121 NNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDL 180
           NNNKSDKKT+ENKKKPKEPPVTTAVLKVALHCQGCIEKI R+T+KFKGVQEMS+D+QK+L
Sbjct: 121 NNNKSDKKTEENKKKPKEPPVTTAVLKVALHCQGCIEKIQRITTKFKGVQEMSLDKQKEL 180

Query: 181 VTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKD-DNNNNKND--SGDKKDIPAGDVE 240
           VTVKGTMDVKALS  LTERLKR VEIVP KKEKDKD +NNNNKN+   G+KK+ PA    
Sbjct: 181 VTVKGTMDVKALSGCLTERLKRAVEIVPTKKEKDKDNNNNNNKNEGGGGNKKEPPAAG-- 240

Query: 241 KNNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDG---EECGAGAKMEGNKMEYMGMG 300
               EG GN N NGGGKKKKKGGNGNG G+G G G     EE G G KMEG KMEYMGMG
Sbjct: 241 ----EGDGNSNENGGGKKKKKGGNGNGGGDGGGDGGATGMEEGGGGGKMEGYKMEYMGMG 300

Query: 301 GIGYGYPYGLSNGYGYGVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
           G G GY YGL+       GYGYGYG GG+VGENLHAPQLFSDENPNAC IM
Sbjct: 301 G-GIGYGYGLN-------GYGYGYGAGGVVGENLHAPQLFSDENPNACWIM 333

BLAST of Spg032591 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.7e-63
Identity = 172/340 (50.59%), Postives = 212/340 (62.35%), Query Frame = 0

Query: 8   KKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQSVKPEIEC 67
           +KKNE +    GG   KK E   +TVVLKVDMHCEGCA++I+KCV+ F+GV++VK E   
Sbjct: 3   EKKNEGDNKKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESAT 62

Query: 68  NKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTSTNNNKSDK 127
            KLTV G  +D  KLREKL  KTKKKVDL+SPQPKKEK+ + K  NDED+     ++  K
Sbjct: 63  GKLTVTG-ALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDK---KKSEEKK 122

Query: 128 KTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDLVTVKGTM 187
           K D N KKPKE PVTTAVLK+  HCQGCI KI +  +K KGV  +++D++K+L+TVKGTM
Sbjct: 123 KPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTM 182

Query: 188 DVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSGDKKDIPAGDVEKNNTEGGGNG 247
           DVK L   L+E+LKR VEIVP KKEKDK+    N N++G+KK            +GGG  
Sbjct: 183 DVKKLVEILSEKLKRAVEIVPPKKEKDKE----NGNENGEKK------------KGGG-- 242

Query: 248 NGNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGMGGIGYGYPYGLSNG 307
            G+GGGK+K         GN  GGG+G           N MEYM                
Sbjct: 243 -GDGGGKEK--------TGN-KGGGEGV----------NMMEYMAA-------------- 283

Query: 308 YGYGVGYGYGYGPGGLVGENL--HAPQLFSDENPNACSIM 346
                 YGYGY PGG  G  +  HAPQ+FSDENPNAC +M
Sbjct: 303 ---QPAYGYGYYPGGPYGYPIQAHAPQIFSDENPNACVVM 283

BLAST of Spg032591 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.8e-33
Identity = 89/212 (41.98%), Postives = 135/212 (63.68%), Query Frame = 0

Query: 16  NGDGGGGAKKKEEN---PLTVVLKVDMHCEGCANKIIKCVKGFEGVQSVKPEIECNKLTV 75
           +GD     KKK+ N   P+ VVLK+D HC+GC  +I++  +  EGV++V+ + + NKLT+
Sbjct: 11  HGDVEEEKKKKQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEGVETVRADPDSNKLTL 70

Query: 76  MGKKIDASKLREKLSNKTKKKVDLISPQPKKE-KDSKPKENNDEDQTSTNNNKSDKKTDE 135
           +G  +D  K+ EKL  K+KKKV+LISP+PKK+ K++  K+ ND+ QT             
Sbjct: 71  IGFIMDPVKIAEKLQKKSKKKVELISPKPKKDTKENNEKKANDKTQTVV----------- 130

Query: 136 NKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDLVTVKGTMDVKA 195
                    VTT VLKV   C GCI++I +  S  KGV ++ +D++K+ VTV GTMD+K+
Sbjct: 131 --------AVTTVVLKVNCSCDGCIKRIQKAVSTTKGVYQVKMDKEKETVTVMGTMDIKS 190

Query: 196 LSVSLTERLKRTVEIVPMKKEKDKDDNNNNKN 224
           ++ +L  +LK+TV++VP KK+K KD +N   N
Sbjct: 191 VTDNLKRKLKKTVQVVPEKKKKKKDKDNAEVN 203

BLAST of Spg032591 vs. ExPASy Swiss-Prot
Match: Q9LZF1 (Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=3702 GN=HIPP06 PE=1 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 4.0e-33
Identity = 149/408 (36.52%), Postives = 203/408 (49.75%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGE   KK++   +  G+     K  +    TVV+K+DMHCEGC  KI +  K F+GV+ 
Sbjct: 1   MGE---KKEETATKPQGE----KKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVED 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLIS--PQPKKE-------------- 120
           VK + + NKLTV+G  +D  ++R+K+++K K+ V+L+S    PKKE              
Sbjct: 61  VKIDYKSNKLTVIG-NVDPVEVRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSP 120

Query: 121 -KDSKPKENNDEDQTSTNNNKSDKKTDENKKKPKEPP---VTTAVLKVALHCQGCIEKIH 180
             + KP E            K +KK +E +KK   PP    +T VLK  LHC+GC  KI 
Sbjct: 121 AAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIK 180

Query: 181 RVTSKFKGVQEMSVDRQKDLVTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDDNNN 240
           R+ +K KGV  +++D  KDLV VKG +DVK L+  L E+LKRTVE+VP KK+        
Sbjct: 181 RIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAA 240

Query: 241 NKNDS-GDKKDIPAGDVEKNNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEECGA 300
               + G+KKD  AG  EK   +  G    +GGG+KKK+   G G G G GGGD      
Sbjct: 241 AAAPAGGEKKDKVAG--EKKEIKDVGEKKVDGGGEKKKEVAVGGGGGGGGGGGD------ 300

Query: 301 GAKMEGNKMEYMGMG-----------GIGYGYPYGLSNG--------------------- 346
           G  M+  K EY G G           G  YG  + +  G                     
Sbjct: 301 GGAMDVKKSEYNGYGYPPQPMYYYPEGQVYGQQHYMMQGQSSQSYVQEPYSNQGYVQESY 360

BLAST of Spg032591 vs. ExPASy Swiss-Prot
Match: Q9SJL2 (Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=3702 GN=HIPP05 PE=1 SV=2)

HSP 1 Score: 136.3 bits (342), Expect = 6.3e-31
Identity = 143/374 (38.24%), Postives = 191/374 (51.07%), Query Frame = 0

Query: 5   VPKKKKNENEKNGDGGGGAKKKEENPLTV---VLKVDMHCEGCANKIIKCVKGFEGVQSV 64
           VP +  +   K+G G   A         V   V KVDMHCEGCA KI + VK F+GV+ V
Sbjct: 21  VPVETTDGKPKSGGGDSAAAAAPPVAAVVSAFVYKVDMHCEGCAKKIKRMVKHFDGVKDV 80

Query: 65  KPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTSTN 124
             +   NKL V+G KID  KL+EKL  KTK+KV L +P PK E          +      
Sbjct: 81  TADTGGNKLLVVG-KIDPVKLQEKLEEKTKRKVVLANPPPKVEGPVAAAVGEKK------ 140

Query: 125 NNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDLV 184
            +  DK+       P  P  +   LK+ LHC+GCI+KI ++  K KGV+ +++D  KD+V
Sbjct: 141 ADGGDKEAAPPAPAPAAPKESVVPLKIRLHCEGCIQKIKKIILKIKGVETVAIDGAKDVV 200

Query: 185 TVKGTMDVKALSVSLTERLKRTVE-IVPMKKEKDKDDNNNNKNDSGD-KKDIPAGDVEKN 244
           TVKGT+DVK L   LT++LKRTVE +VP KK+    +N   +  + D KK+ P+  V + 
Sbjct: 201 TVKGTIDVKELVPLLTKKLKRTVEPLVPAKKDDGAAENKKTEAAAPDAKKEAPSAGVNEA 260

Query: 245 NTEGGGNGN-----GNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKME---GNKMEYM 304
             EG   G      G+GG KKK+ G  G        GG+ ++ G G        NKM+Y 
Sbjct: 261 KKEGSDGGEKKKEVGDGGEKKKEGGDGGEKKKEAGDGGEKKKDGGGVPAPVAMVNKMDYY 320

Query: 305 GMG-----------GIGYGYPYGLSNGYGYGV------GYGYGYGPGGLV---GENLHAP 346
           G             G  YG  Y ++ G  Y V      G GY Y     V     N++AP
Sbjct: 321 GYSAYPTAPMHWQEGHVYGQSYSMT-GQNYPVGGQSYPGSGYNYASESYVPYAQPNVNAP 380

BLAST of Spg032591 vs. ExPASy Swiss-Prot
Match: Q9C5D3 (Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=3702 GN=HIPP07 PE=1 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.6e-18
Identity = 111/358 (31.01%), Postives = 165/358 (46.09%), Query Frame = 0

Query: 7   KKKKNENEKNGDGGGGAKKKEEN-------------PLTVVLKVDMHCEGCANKIIKCVK 66
           KK ++ ++K  +G      KE++             P  VVLKV MHCEGCA K+ +C+K
Sbjct: 36  KKGEDSDKKPQEGESNKDSKEDSAPAAPEAPAPPPPPQEVVLKVYMHCEGCARKVRRCLK 95

Query: 67  GFEGVQSVKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENN 126
           GFEGV+ V  + +  K+ V G+K D  K+  ++  KT ++V L+SP P       P++  
Sbjct: 96  GFEGVEDVMTDCKTGKVVVKGEKADPLKVLARVQRKTHRQVQLLSPIP--PPPPPPEKKA 155

Query: 127 DEDQTSTNNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMS 186
           +ED           K    +KK + P V T VLKV +HC+ C  +I +   + KGV+   
Sbjct: 156 EED-----------KPIVEEKKVEPPVVVTVVLKVHMHCEACATEIKKRIMRMKGVESAE 215

Query: 187 VDRQKDLVTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSGDKKDIPA 246
            D +   VTVKG  + + L   + +R  +   I+ +                G+KK+   
Sbjct: 216 SDLKSSQVTVKGVFEPQKLVEYVYKRTGKHAAIMKIDPPPPPPPEEAAAAAEGEKKEEEK 275

Query: 247 GDVEKNNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGM 306
           G+ E    EG  +       KK+  GG G G    NGGG+ E    G  +E  K+E    
Sbjct: 276 GEGESKGEEGKDDKAKTDEEKKEGDGGKGEGEAADNGGGEEE----GKVVEVRKIE---- 335

Query: 307 GGIGYGYPYGLSNGYGYGVGYGYGYGPGGL------VGENLHAPQLFSDENPNACSIM 346
                  PY           Y Y Y P  +      +  + + PQLFSDENPNAC++M
Sbjct: 336 ------NPY-----------YYYYYQPPRVAIPPMEMPPHAYPPQLFSDENPNACTVM 355

BLAST of Spg032591 vs. ExPASy TrEMBL
Match: A0A0A0LSA4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G103270 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.6e-128
Identity = 275/348 (79.02%), Postives = 295/348 (84.77%), Query Frame = 0

Query: 1   MGE-AVPKKKKNENEKNGDGGGG-AKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGV 60
           MGE AV KKKKN+NEKNGDGGGG  KKKEE P T+VLK+DMHCEGCANKI KCVKGFEGV
Sbjct: 1   MGEAAVQKKKKNDNEKNGDGGGGEGKKKEEIPFTIVLKIDMHCEGCANKITKCVKGFEGV 60

Query: 61  QSVKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQT 120
           QSVK EI+ NKLTVMGKKIDA+KLREKLSNKTKKKVDLISPQPKKEKDSKPK+  D+DQT
Sbjct: 61  QSVKAEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQT 120

Query: 121 STNNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQK 180
           S+NNNKSDKKTDENKKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGVQEMSVD+QK
Sbjct: 121 SSNNNKSDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDKQK 180

Query: 181 DLVTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSGDKKDIPAGDVEK 240
           D V VKGTMDVKAL  SL+ERLKRTVEIVP KKEK+K+ +NN K   G       GD +K
Sbjct: 181 DSVMVKGTMDVKALIGSLSERLKRTVEIVPAKKEKEKEKDNNKKEGGG------GGDEKK 240

Query: 241 NNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEE-CGAGAKMEGNKMEYMGMGGIG 300
           ++T G G+GNGNGGGKKKKKGGNG       GGGDGEE  G G KMEGNKMEYMGMGGIG
Sbjct: 241 DSTTGDGDGNGNGGGKKKKKGGNG-------GGGDGEEGGGGGGKMEGNKMEYMGMGGIG 300

Query: 301 YGYPYGLSNGYGYGVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
           YGY Y    GYGYG+  GYGYGP G+VGENLHAPQLFSDENPNAC IM
Sbjct: 301 YGYGY----GYGYGMNTGYGYGPSGIVGENLHAPQLFSDENPNACFIM 331

BLAST of Spg032591 vs. ExPASy TrEMBL
Match: A0A5A7U7D8 (Heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold60G002580 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 7.6e-128
Identity = 275/348 (79.02%), Postives = 291/348 (83.62%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGEAV KKKKN+NEKNGDG GG KKKE+ P T+VLK+DMHCEGCANKI KCVKGFEGVQS
Sbjct: 1   MGEAVQKKKKNDNEKNGDGEGGGKKKEDIPFTIVLKIDMHCEGCANKITKCVKGFEGVQS 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTST 120
           VK EI+ NKLTVMGKKIDA+KLREKLSNKTKKKVDLISPQPKKEKDSKPK+  D+DQTS+
Sbjct: 61  VKAEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSS 120

Query: 121 NNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDL 180
           NNNK DKKTDENKKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGVQEMSVDRQKD 
Sbjct: 121 NNNKFDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDRQKDS 180

Query: 181 VTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSG---DKKDIPAGDVE 240
           V VKGTMDVKAL  SL+ERLKR VEIVP KKEK+K +  NNKN+ G   DKKD P GD  
Sbjct: 181 VMVKGTMDVKALIGSLSERLKRPVEIVPAKKEKEK-EKENNKNEGGGGDDKKDSPTGD-- 240

Query: 241 KNNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGMGGIG 300
               +G GNGNGNGGGKKKKKGGNG G     GGG     G G KMEGNKMEYMGMGGIG
Sbjct: 241 ---GDGNGNGNGNGGGKKKKKGGNGGGGEGEEGGG-----GGGGKMEGNKMEYMGMGGIG 300

Query: 301 YGYPYGLSNGYGYGVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
           YGY      GYGYG+  GYGYGPGGLVGENLHAPQLFSDENPNAC IM
Sbjct: 301 YGY------GYGYGLNTGYGYGPGGLVGENLHAPQLFSDENPNACFIM 331

BLAST of Spg032591 vs. ExPASy TrEMBL
Match: A0A1S3CIH0 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=3656 GN=LOC103501337 PE=4 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 7.6e-128
Identity = 275/348 (79.02%), Postives = 291/348 (83.62%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGEAV KKKKN+NEKNGDG GG KKKE+ P T+VLK+DMHCEGCANKI KCVKGFEGVQS
Sbjct: 1   MGEAVQKKKKNDNEKNGDGEGGGKKKEDIPFTIVLKIDMHCEGCANKITKCVKGFEGVQS 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTST 120
           VK EI+ NKLTVMGKKIDA+KLREKLSNKTKKKVDLISPQPKKEKDSKPK+  D+DQTS+
Sbjct: 61  VKAEIDGNKLTVMGKKIDATKLREKLSNKTKKKVDLISPQPKKEKDSKPKDKIDDDQTSS 120

Query: 121 NNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDL 180
           NNNK DKKTDENKKKPKEPPVTTAVLKV LHCQGCIEKI RVT+KFKGVQEMSVDRQKD 
Sbjct: 121 NNNKFDKKTDENKKKPKEPPVTTAVLKVPLHCQGCIEKIQRVTTKFKGVQEMSVDRQKDS 180

Query: 181 VTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSG---DKKDIPAGDVE 240
           V VKGTMDVKAL  SL+ERLKR VEIVP KKEK+K +  NNKN+ G   DKKD P GD  
Sbjct: 181 VMVKGTMDVKALIGSLSERLKRPVEIVPAKKEKEK-EKENNKNEGGGGDDKKDSPTGD-- 240

Query: 241 KNNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGMGGIG 300
               +G GNGNGNGGGKKKKKGGNG G     GGG     G G KMEGNKMEYMGMGGIG
Sbjct: 241 ---GDGNGNGNGNGGGKKKKKGGNGGGGEGEEGGG-----GGGGKMEGNKMEYMGMGGIG 300

Query: 301 YGYPYGLSNGYGYGVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
           YGY      GYGYG+  GYGYGPGGLVGENLHAPQLFSDENPNAC IM
Sbjct: 301 YGY------GYGYGLNTGYGYGPGGLVGENLHAPQLFSDENPNACFIM 331

BLAST of Spg032591 vs. ExPASy TrEMBL
Match: A0A6J1HJ75 (heavy metal-associated isoprenylated plant protein 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464956 PE=4 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 6.2e-122
Identity = 275/351 (78.35%), Postives = 291/351 (82.91%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGE   KK KNENEKNGDGGGG KKKEENP TVVLKVDMHCEGCANKI KCVKGFEGVQ+
Sbjct: 1   MGE---KKTKNENEKNGDGGGG-KKKEENPFTVVLKVDMHCEGCANKITKCVKGFEGVQT 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTST 120
           VK EIE NKLTV G+KIDASKL EKLSNKTKKKVDLISPQ KKEKDSKPK   DEDQTS+
Sbjct: 61  VKAEIEGNKLTVTGEKIDASKLLEKLSNKTKKKVDLISPQSKKEKDSKPKVKGDEDQTSS 120

Query: 121 NNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDL 180
           NNNKSDKKT+ENKKKPKEPPVTTAVLKVALHCQGCIEKI R+T+KFKGVQEMS+D+QK+L
Sbjct: 121 NNNKSDKKTEENKKKPKEPPVTTAVLKVALHCQGCIEKIQRITTKFKGVQEMSLDKQKEL 180

Query: 181 VTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKD-DNNNNKND--SGDKKDIPAGDVE 240
           VTVKGTMDVKALS  LTERLKR VEIVP KKEKDKD +NNNNKN+   G+KK+ PA    
Sbjct: 181 VTVKGTMDVKALSGCLTERLKRAVEIVPTKKEKDKDNNNNNNKNEGGGGNKKEPPAAG-- 240

Query: 241 KNNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDG---EECGAGAKMEGNKMEYMGMG 300
               EG GN N NGGGKKKKKGGNGNG G+G G G     EE G G KMEG KMEYMGMG
Sbjct: 241 ----EGDGNSNENGGGKKKKKGGNGNGGGDGGGDGGATGMEEGGGGGKMEGYKMEYMGMG 300

Query: 301 GIGYGYPYGLSNGYGYGVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
           G G GY YGL+       GYGYGYG GG+VGENLHAPQLFSDENPNAC IM
Sbjct: 301 G-GIGYGYGLN-------GYGYGYGAGGVVGENLHAPQLFSDENPNACWIM 333

BLAST of Spg032591 vs. ExPASy TrEMBL
Match: A0A6J1HML5 (heavy metal-associated isoprenylated plant protein 3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464956 PE=4 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 8.4e-119
Identity = 270/348 (77.59%), Postives = 285/348 (81.90%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGE   KK KNENEKNGDGGGG KKKEENP TVVLKVDMHCEGCANKI KCVKGFEGVQ+
Sbjct: 1   MGE---KKTKNENEKNGDGGGG-KKKEENPFTVVLKVDMHCEGCANKITKCVKGFEGVQT 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTST 120
           VK EIE NKLTV G+KIDASKL EKLSNKTKKKVDLISPQ KKEKDSKPK   DEDQTS+
Sbjct: 61  VKAEIEGNKLTVTGEKIDASKLLEKLSNKTKKKVDLISPQSKKEKDSKPKVKGDEDQTSS 120

Query: 121 NNNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDL 180
           NNNKSDKKT+ENKKKPKEPPVTTAVLKVALHCQGCIEKI R+T+KFKGVQEMS+D+QK+L
Sbjct: 121 NNNKSDKKTEENKKKPKEPPVTTAVLKVALHCQGCIEKIQRITTKFKGVQEMSLDKQKEL 180

Query: 181 VTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKD-DNNNNKND--SGDKKDIPAGDVE 240
           VTVKGTMDVKALS  LTERLKR VEIVP KKEKDKD +NNNNKN+   G+KK+ PA    
Sbjct: 181 VTVKGTMDVKALSGCLTERLKRAVEIVPTKKEKDKDNNNNNNKNEGGGGNKKEPPAAG-- 240

Query: 241 KNNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGMGGIG 300
               EG GN N NGGGKKKKKGGNG   G   GGG       G KMEG KMEYMGMGG G
Sbjct: 241 ----EGDGNSNENGGGKKKKKGGNGGATGMEEGGG-------GGKMEGYKMEYMGMGG-G 300

Query: 301 YGYPYGLSNGYGYGVGYGYGYGPGGLVGENLHAPQLFSDENPNACSIM 346
            GY YGL+       GYGYGYG GG+VGENLHAPQLFSDENPNAC IM
Sbjct: 301 IGYGYGLN-------GYGYGYGAGGVVGENLHAPQLFSDENPNACWIM 323

BLAST of Spg032591 vs. TAIR 10
Match: AT5G60800.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 244.6 bits (623), Expect = 1.2e-64
Identity = 172/340 (50.59%), Postives = 212/340 (62.35%), Query Frame = 0

Query: 8   KKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQSVKPEIEC 67
           +KKNE +    GG   KK E   +TVVLKVDMHCEGCA++I+KCV+ F+GV++VK E   
Sbjct: 3   EKKNEGDNKKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESAT 62

Query: 68  NKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTSTNNNKSDK 127
            KLTV G  +D  KLREKL  KTKKKVDL+SPQPKKEK+ + K  NDED+     ++  K
Sbjct: 63  GKLTVTG-ALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDK---KKSEEKK 122

Query: 128 KTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDLVTVKGTM 187
           K D N KKPKE PVTTAVLK+  HCQGCI KI +  +K KGV  +++D++K+L+TVKGTM
Sbjct: 123 KPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTM 182

Query: 188 DVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSGDKKDIPAGDVEKNNTEGGGNG 247
           DVK L   L+E+LKR VEIVP KKEKDK+    N N++G+KK            +GGG  
Sbjct: 183 DVKKLVEILSEKLKRAVEIVPPKKEKDKE----NGNENGEKK------------KGGG-- 242

Query: 248 NGNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGMGGIGYGYPYGLSNG 307
            G+GGGK+K         GN  GGG+G           N MEYM                
Sbjct: 243 -GDGGGKEK--------TGN-KGGGEGV----------NMMEYMAA-------------- 283

Query: 308 YGYGVGYGYGYGPGGLVGENL--HAPQLFSDENPNACSIM 346
                 YGYGY PGG  G  +  HAPQ+FSDENPNAC +M
Sbjct: 303 ---QPAYGYGYYPGGPYGYPIQAHAPQIFSDENPNACVVM 283

BLAST of Spg032591 vs. TAIR 10
Match: AT5G60800.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 243.0 bits (619), Expect = 3.4e-64
Identity = 171/340 (50.29%), Postives = 212/340 (62.35%), Query Frame = 0

Query: 8   KKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQSVKPEIEC 67
           +KKNE +    GG   KK E   +TVVLKVDMHCEGCA++I+KCV+ F+GV++VK E   
Sbjct: 3   EKKNEGDNKKKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESAT 62

Query: 68  NKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTSTNNNKSDK 127
            KLTV G  +D  KLREKL  KTKKKVDL+SPQPKKEK+ + K  NDED+     ++  K
Sbjct: 63  GKLTVTG-ALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDK---KKSEEKK 122

Query: 128 KTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDLVTVKGTM 187
           K D N KKPKE PVTTAVLK+  HCQGCI KI +  +K KGV  +++D++K+L+TVKGTM
Sbjct: 123 KPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTM 182

Query: 188 DVKALSVSLTERLKRTVEIVPMKKEKDKDDNNNNKNDSGDKKDIPAGDVEKNNTEGGGNG 247
           DVK L   L+E+LKR VEIVP KKEKDK+    N N++G+KK            +GGG  
Sbjct: 183 DVKKLVEILSEKLKRAVEIVPPKKEKDKE----NGNENGEKK------------KGGG-- 242

Query: 248 NGNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGMGGIGYGYPYGLSNG 307
            G+GGGK+K         GN  GGG+G           N MEYM                
Sbjct: 243 -GDGGGKEK--------TGN-KGGGEGV----------NMMEYMAA-------------- 283

Query: 308 YGYGVGYGYGYGPGGLVGENL--HAPQLFSDENPNACSIM 346
                 YGYGY PGG  G  +  HAPQ+FSDENPNAC ++
Sbjct: 303 ---QPAYGYGYYPGGPYGYPIQAHAPQIFSDENPNACVVI 283

BLAST of Spg032591 vs. TAIR 10
Match: AT5G03380.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 143.7 bits (361), Expect = 2.8e-34
Identity = 149/408 (36.52%), Postives = 203/408 (49.75%), Query Frame = 0

Query: 1   MGEAVPKKKKNENEKNGDGGGGAKKKEENPLTVVLKVDMHCEGCANKIIKCVKGFEGVQS 60
           MGE   KK++   +  G+     K  +    TVV+K+DMHCEGC  KI +  K F+GV+ 
Sbjct: 1   MGE---KKEETATKPQGE----KKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVED 60

Query: 61  VKPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLIS--PQPKKE-------------- 120
           VK + + NKLTV+G  +D  ++R+K+++K K+ V+L+S    PKKE              
Sbjct: 61  VKIDYKSNKLTVIG-NVDPVEVRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSP 120

Query: 121 -KDSKPKENNDEDQTSTNNNKSDKKTDENKKKPKEPP---VTTAVLKVALHCQGCIEKIH 180
             + KP E            K +KK +E +KK   PP    +T VLK  LHC+GC  KI 
Sbjct: 121 AAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIK 180

Query: 181 RVTSKFKGVQEMSVDRQKDLVTVKGTMDVKALSVSLTERLKRTVEIVPMKKEKDKDDNNN 240
           R+ +K KGV  +++D  KDLV VKG +DVK L+  L E+LKRTVE+VP KK+        
Sbjct: 181 RIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAA 240

Query: 241 NKNDS-GDKKDIPAGDVEKNNTEGGGNGNGNGGGKKKKKGGNGNGNGNGNGGGDGEECGA 300
               + G+KKD  AG  EK   +  G    +GGG+KKK+   G G G G GGGD      
Sbjct: 241 AAAPAGGEKKDKVAG--EKKEIKDVGEKKVDGGGEKKKEVAVGGGGGGGGGGGD------ 300

Query: 301 GAKMEGNKMEYMGMG-----------GIGYGYPYGLSNG--------------------- 346
           G  M+  K EY G G           G  YG  + +  G                     
Sbjct: 301 GGAMDVKKSEYNGYGYPPQPMYYYPEGQVYGQQHYMMQGQSSQSYVQEPYSNQGYVQESY 360

BLAST of Spg032591 vs. TAIR 10
Match: AT5G03380.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 142.9 bits (359), Expect = 4.8e-34
Identity = 139/374 (37.17%), Postives = 188/374 (50.27%), Query Frame = 0

Query: 35  LKVDMHCEGCANKIIKCVKGFEGVQSVKPEIECNKLTVMGKKIDASKLREKLSNKTKKKV 94
           +K+DMHCEGC  KI +  K F+GV+ VK + + NKLTV+G  +D  ++R+K+++K K+ V
Sbjct: 1   MKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIG-NVDPVEVRDKVADKIKRPV 60

Query: 95  DLIS--PQPKKE---------------KDSKPKENNDEDQTSTNNNKSDKKTDENKKKPK 154
           +L+S    PKKE                + KP E            K +KK +E +KK  
Sbjct: 61  ELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKAS 120

Query: 155 EPP---VTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDLVTVKGTMDVKALSV 214
            PP    +T VLK  LHC+GC  KI R+ +K KGV  +++D  KDLV VKG +DVK L+ 
Sbjct: 121 PPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTP 180

Query: 215 SLTERLKRTVEIVPMKKEKDKDDNNNNKNDS-GDKKDIPAGDVEKNNTEGGGNGNGNGGG 274
            L E+LKRTVE+VP KK+            + G+KKD  AG  EK   +  G    +GGG
Sbjct: 181 YLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDKVAG--EKKEIKDVGEKKVDGGG 240

Query: 275 KKKKKGGNGNGNGNGNGGGDGEECGAGAKMEGNKMEYMGMG-----------GIGYGYPY 334
           +KKK+   G G G G GGGD      G  M+  K EY G G           G  YG  +
Sbjct: 241 EKKKEVAVGGGGGGGGGGGD------GGAMDVKKSEYNGYGYPPQPMYYYPEGQVYGQQH 300

Query: 335 GLSNG-----------------------YGYGVGYGYGYGPGGLVGE--------NLHAP 346
            +  G                        GYG GYG    P   + +        ++ AP
Sbjct: 301 YMMQGQSSQSYVQEPYSNQGYVQESYMNQGYGQGYGQEAPPPPYMNQQGYADPYGHMRAP 360

BLAST of Spg032591 vs. TAIR 10
Match: AT2G36950.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 4.5e-32
Identity = 143/374 (38.24%), Postives = 191/374 (51.07%), Query Frame = 0

Query: 5   VPKKKKNENEKNGDGGGGAKKKEENPLTV---VLKVDMHCEGCANKIIKCVKGFEGVQSV 64
           VP +  +   K+G G   A         V   V KVDMHCEGCA KI + VK F+GV+ V
Sbjct: 21  VPVETTDGKPKSGGGDSAAAAAPPVAAVVSAFVYKVDMHCEGCAKKIKRMVKHFDGVKDV 80

Query: 65  KPEIECNKLTVMGKKIDASKLREKLSNKTKKKVDLISPQPKKEKDSKPKENNDEDQTSTN 124
             +   NKL V+G KID  KL+EKL  KTK+KV L +P PK E          +      
Sbjct: 81  TADTGGNKLLVVG-KIDPVKLQEKLEEKTKRKVVLANPPPKVEGPVAAAVGEKK------ 140

Query: 125 NNKSDKKTDENKKKPKEPPVTTAVLKVALHCQGCIEKIHRVTSKFKGVQEMSVDRQKDLV 184
            +  DK+       P  P  +   LK+ LHC+GCI+KI ++  K KGV+ +++D  KD+V
Sbjct: 141 ADGGDKEAAPPAPAPAAPKESVVPLKIRLHCEGCIQKIKKIILKIKGVETVAIDGAKDVV 200

Query: 185 TVKGTMDVKALSVSLTERLKRTVE-IVPMKKEKDKDDNNNNKNDSGD-KKDIPAGDVEKN 244
           TVKGT+DVK L   LT++LKRTVE +VP KK+    +N   +  + D KK+ P+  V + 
Sbjct: 201 TVKGTIDVKELVPLLTKKLKRTVEPLVPAKKDDGAAENKKTEAAAPDAKKEAPSAGVNEA 260

Query: 245 NTEGGGNGN-----GNGGGKKKKKGGNGNGNGNGNGGGDGEECGAGAKME---GNKMEYM 304
             EG   G      G+GG KKK+ G  G        GG+ ++ G G        NKM+Y 
Sbjct: 261 KKEGSDGGEKKKEVGDGGEKKKEGGDGGEKKKEAGDGGEKKKDGGGVPAPVAMVNKMDYY 320

Query: 305 GMG-----------GIGYGYPYGLSNGYGYGV------GYGYGYGPGGLV---GENLHAP 346
           G             G  YG  Y ++ G  Y V      G GY Y     V     N++AP
Sbjct: 321 GYSAYPTAPMHWQEGHVYGQSYSMT-GQNYPVGGQSYPGSGYNYASESYVPYAQPNVNAP 380