Spg032589 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg032589
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like
Location	scaffold11: 5575541 .. 5577277 (+)
RNA-Seq Expression	Spg032589
Synteny	Spg032589

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACGAACGGCAAGATGAAGTATTGGGTTTCATCAAACAAGTACTGCAAGGGCGGTGGTTTTCGGTATTCGCCTCGTTTTTGATGATGATCGGCAACGGTTCGACTTACATATTTGGAACCTACTCCAAAGTGATAAAGACCCAATTCGATTACAGTCAAACCCAAGTTAACACGTTGGGATTTGCGAAAGATCTCGGCTCCAACGTCGGAGTTTTCGCCGGCCTTCTCGCCGAGGTGGCTCCGTTGTGGGTTCAATTCATGATCGGCGCAATTCTTAACTTCTCCGGCTACTTCATGATTTGGCTCTCCATCACTCGTCGTATCCCCAAGCCCGAATTCTGGCAACTGTTCCTTTACATTTGCATCGGCACTAATTCATCAAATTTCGCCAACACCGCCATTATGGTCACAAGTGTTAGAAACTTCCCCGACCGACGAGGAATTATTTTGGGCCTTCTTAAAGGGTTTGTTGGTATTGGCGGTGCCATCATAACCCAACTGTACTTGGCCATCTACGGCCACGATGACCCATCGGATCTAGTTCTTCTTTTCTCTTGGCTTCCCTCAACCGTAATCCTCCTCCTTTTCTACTCGGTTCGACCTATCAAGATCCGTAAACATCCCGAAGAGCTCAAAGTCTTTTACCATTTACTTTATGTGTCCATAGTTCTGGCGCTTTTCATCTTGTTTTTGACCATAACTCAGAAACAAGTCACTTTCTCTCACGCGGGATACGCGAGTGGCGCATCCGTCATCGTTGGCTTGCTGAGCCTACACCTTCTGATCGCCGTTAGAGAAGAATTCTTGCTGTACAACCTCAAAAAACAAAACTGTGATCCATCTGCCATTGTTTCCATTGTTGAGCAAAAGCCTCCGTTGGCAACTGCCCCTGAGAATTCGATGTCCACGTTAGAGGAATTTGGAGAGGTTTCGCCGTCGTGTTTTTCAGACATATTTAACAAGCCTCCAAGAGGGGAAGATTTCACCATCTTGCAAGCTATTTTCAGCAAAGATATGGCGCTGATCTGTTTAGCTACTTTTTCTGGATGTGGGTCATCTCTTGCGGCCATTGATAATTTAGGCCAAATTGGCGAATCACTTGGCTATCCACCGAAAGCCATAAGCATCTTCGTTTCTTGGGTTTCGGTTTTCAATTTCTTCGGCCGAGTCTGCTCTGGTTTCATCTCTGAGGCATTGATGGTCAAATACAAAATGCCTCGTCCACTCCTGTTCGCTTTTGCCTTTCTCATAACTTGCATCGGCCAGCTTTGCATCGCCTATCCATCTCCGGGTTCGGTCTACATGGCTTCATTGATTATTGGGTTTGGTTTTGGAGCTCAAAATCCGTTGCTATTCGCCGTAATCTCTGACCTTTTTGGGCTCAAACATTACTCGACGTTGTTCAATTGTGGGCAATTGGCAGTCCCATTAGGGTCTTATTTACTAAATGTGGACGTAGTGGGGAAATTGTACGATAGGGAAGCTTTACGTGAAGGTAGGAGGACGATGGGCAAGGGGATAACTTGCACGGGAGCTCATTGCTTTAGTGGGTCTTTCACAATTTTGGCTGCATTCACATTGTTTGGAGCTTTGACTATGCTCGTGTTGGCTTATAAAACTCGAGAATTTTACAGAGGCGACATCTATAAGAAGTACAGAGAGGAAATGTGGATCCCGCAAACGAAAATGGAGTTCTACTGTTTAGATAACAAGAAGAACATTGATGACTAA

mRNA sequence

ATGGCCAACGAACGGCAAGATGAAGTATTGGGTTTCATCAAACAAGTACTGCAAGGGCGGTGGTTTTCGGTATTCGCCTCGTTTTTGATGATGATCGGCAACGGTTCGACTTACATATTTGGAACCTACTCCAAAGTGATAAAGACCCAATTCGATTACAGTCAAACCCAAGTTAACACGTTGGGATTTGCGAAAGATCTCGGCTCCAACGTCGGAGTTTTCGCCGGCCTTCTCGCCGAGGTGGCTCCGTTGTGGGTTCAATTCATGATCGGCGCAATTCTTAACTTCTCCGGCTACTTCATGATTTGGCTCTCCATCACTCGTCGTATCCCCAAGCCCGAATTCTGGCAACTGTTCCTTTACATTTGCATCGGCACTAATTCATCAAATTTCGCCAACACCGCCATTATGGTCACAAGTGTTAGAAACTTCCCCGACCGACGAGGAATTATTTTGGGCCTTCTTAAAGGGTTTGTTGGTATTGGCGGTGCCATCATAACCCAACTGTACTTGGCCATCTACGGCCACGATGACCCATCGGATCTAGTTCTTCTTTTCTCTTGGCTTCCCTCAACCGTAATCCTCCTCCTTTTCTACTCGGTTCGACCTATCAAGATCCGTAAACATCCCGAAGAGCTCAAAGTCTTTTACCATTTACTTTATGTGTCCATAGTTCTGGCGCTTTTCATCTTGTTTTTGACCATAACTCAGAAACAAGTCACTTTCTCTCACGCGGGATACGCGAGTGGCGCATCCGTCATCGTTGGCTTGCTGAGCCTACACCTTCTGATCGCCGTTAGAGAAGAATTCTTGCTGTACAACCTCAAAAAACAAAACTGTGATCCATCTGCCATTGTTTCCATTGTTGAGCAAAAGCCTCCGTTGGCAACTGCCCCTGAGAATTCGATGTCCACGTTAGAGGAATTTGGAGAGGTTTCGCCGTCGTGTTTTTCAGACATATTTAACAAGCCTCCAAGAGGGGAAGATTTCACCATCTTGCAAGCTATTTTCAGCAAAGATATGGCGCTGATCTGTTTAGCTACTTTTTCTGGATGTGGGTCATCTCTTGCGGCCATTGATAATTTAGGCCAAATTGGCGAATCACTTGGCTATCCACCGAAAGCCATAAGCATCTTCGTTTCTTGGGTTTCGGTTTTCAATTTCTTCGGCCGAGTCTGCTCTGGTTTCATCTCTGAGGCATTGATGGTCAAATACAAAATGCCTCGTCCACTCCTGTTCGCTTTTGCCTTTCTCATAACTTGCATCGGCCAGCTTTGCATCGCCTATCCATCTCCGGGTTCGGTCTACATGGCTTCATTGATTATTGGGTTTGGTTTTGGAGCTCAAAATCCGTTGCTATTCGCCGTAATCTCTGACCTTTTTGGGCTCAAACATTACTCGACGTTGTTCAATTGTGGGCAATTGGCAGTCCCATTAGGGTCTTATTTACTAAATGTGGACGTAGTGGGGAAATTGTACGATAGGGAAGCTTTACGTGAAGGTAGGAGGACGATGGGCAAGGGGATAACTTGCACGGGAGCTCATTGCTTTAGTGGGTCTTTCACAATTTTGGCTGCATTCACATTGTTTGGAGCTTTGACTATGCTCGTGTTGGCTTATAAAACTCGAGAATTTTACAGAGGCGACATCTATAAGAAGTACAGAGAGGAAATGTGGATCCCGCAAACGAAAATGGAGTTCTACTGTTTAGATAACAAGAAGAACATTGATGACTAA

Coding sequence (CDS)

ATGGCCAACGAACGGCAAGATGAAGTATTGGGTTTCATCAAACAAGTACTGCAAGGGCGGTGGTTTTCGGTATTCGCCTCGTTTTTGATGATGATCGGCAACGGTTCGACTTACATATTTGGAACCTACTCCAAAGTGATAAAGACCCAATTCGATTACAGTCAAACCCAAGTTAACACGTTGGGATTTGCGAAAGATCTCGGCTCCAACGTCGGAGTTTTCGCCGGCCTTCTCGCCGAGGTGGCTCCGTTGTGGGTTCAATTCATGATCGGCGCAATTCTTAACTTCTCCGGCTACTTCATGATTTGGCTCTCCATCACTCGTCGTATCCCCAAGCCCGAATTCTGGCAACTGTTCCTTTACATTTGCATCGGCACTAATTCATCAAATTTCGCCAACACCGCCATTATGGTCACAAGTGTTAGAAACTTCCCCGACCGACGAGGAATTATTTTGGGCCTTCTTAAAGGGTTTGTTGGTATTGGCGGTGCCATCATAACCCAACTGTACTTGGCCATCTACGGCCACGATGACCCATCGGATCTAGTTCTTCTTTTCTCTTGGCTTCCCTCAACCGTAATCCTCCTCCTTTTCTACTCGGTTCGACCTATCAAGATCCGTAAACATCCCGAAGAGCTCAAAGTCTTTTACCATTTACTTTATGTGTCCATAGTTCTGGCGCTTTTCATCTTGTTTTTGACCATAACTCAGAAACAAGTCACTTTCTCTCACGCGGGATACGCGAGTGGCGCATCCGTCATCGTTGGCTTGCTGAGCCTACACCTTCTGATCGCCGTTAGAGAAGAATTCTTGCTGTACAACCTCAAAAAACAAAACTGTGATCCATCTGCCATTGTTTCCATTGTTGAGCAAAAGCCTCCGTTGGCAACTGCCCCTGAGAATTCGATGTCCACGTTAGAGGAATTTGGAGAGGTTTCGCCGTCGTGTTTTTCAGACATATTTAACAAGCCTCCAAGAGGGGAAGATTTCACCATCTTGCAAGCTATTTTCAGCAAAGATATGGCGCTGATCTGTTTAGCTACTTTTTCTGGATGTGGGTCATCTCTTGCGGCCATTGATAATTTAGGCCAAATTGGCGAATCACTTGGCTATCCACCGAAAGCCATAAGCATCTTCGTTTCTTGGGTTTCGGTTTTCAATTTCTTCGGCCGAGTCTGCTCTGGTTTCATCTCTGAGGCATTGATGGTCAAATACAAAATGCCTCGTCCACTCCTGTTCGCTTTTGCCTTTCTCATAACTTGCATCGGCCAGCTTTGCATCGCCTATCCATCTCCGGGTTCGGTCTACATGGCTTCATTGATTATTGGGTTTGGTTTTGGAGCTCAAAATCCGTTGCTATTCGCCGTAATCTCTGACCTTTTTGGGCTCAAACATTACTCGACGTTGTTCAATTGTGGGCAATTGGCAGTCCCATTAGGGTCTTATTTACTAAATGTGGACGTAGTGGGGAAATTGTACGATAGGGAAGCTTTACGTGAAGGTAGGAGGACGATGGGCAAGGGGATAACTTGCACGGGAGCTCATTGCTTTAGTGGGTCTTTCACAATTTTGGCTGCATTCACATTGTTTGGAGCTTTGACTATGCTCGTGTTGGCTTATAAAACTCGAGAATTTTACAGAGGCGACATCTATAAGAAGTACAGAGAGGAAATGTGGATCCCGCAAACGAAAATGGAGTTCTACTGTTTAGATAACAAGAAGAACATTGATGACTAA

Protein sequence

MANERQDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNTLGFAKDLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFLYICIGTNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPSDLVLLFSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQVTFSHAGYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPENSMSTLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAIDNLGQIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLITCIGQLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLGSYLLNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAYKTREFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD

Homology

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BLAST of Spg032589 vs. NCBI nr
Match: XP_008464773.1 (PREDICTED: uncharacterized protein LOC103502580 [Cucumis melo])

HSP 1 Score: 892.9 bits (2306), Expect = 1.5e-255
Identity = 442/575 (76.87%), Postives = 499/575 (86.78%), Query Frame = 0


Query: 6   QDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNTLGFAK 65
           + E   FIKQV+QGRWFSV+A  +MMIGNG+TYIFGTYSKV+KT+FDYSQTQVN LGFAK
Sbjct: 2   ESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFAK 61

Query: 66  DLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFLYICIG 125
           DLGSNVG+ AGLLAE  P WV F IGA  NF+ YF IWLS+TRRIP+PEFWQ+FL +C G
Sbjct: 62  DLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCFG 121

Query: 126 TNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPSDLVLL 185
           +NSSN+ANTAIMV S+RNFPDRRGIILGLLKG+VGIGGAI+TQ+YL IYG +DPSDLVLL
Sbjct: 122 SNSSNYANTAIMVISLRNFPDRRGIILGLLKGYVGIGGAILTQIYLGIYGPEDPSDLVLL 181

Query: 186 FSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQVTFSHA 245
           F+W PS +ILL+  S+RPI IRKHPEELKVFYHLLYVSI++A+FI FL+ITQKQVTFS A
Sbjct: 182 FAWFPSVLILLISTSIRPIHIRKHPEELKVFYHLLYVSIIIAIFIFFLSITQKQVTFSPA 241

Query: 246 GYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNC--DPSAIVSIVEQKPPLATAPENSM 305
            YASGA V+V LL L LLI  REEFLLY LKKQN   DPS  +SIV+QK    T    S 
Sbjct: 242 AYASGAYVVVALLCLPLLIVCREEFLLYKLKKQNLNRDPSVALSIVDQK---VTNCPKSF 301

Query: 306 STLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAIDNLG 365
           STLEE  E+SPSC S+I NKP RG DFT+LQAIFS DM LICLATFSGCGSSLAAIDNLG
Sbjct: 302 STLEEIAEISPSCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSSLAAIDNLG 361

Query: 366 QIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLITCIG 425
           QIGESLGYP +AISIFVSWVS+FNFFGRV SGFISE +++KYK+PRPL+FAFAFL+TC+G
Sbjct: 362 QIGESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAFAFLLTCLG 421

Query: 426 QLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLGSYL 485
           QLCIAYP PGSVY+AS IIGFGFGAQNPLLFAVIS++FGLKHYS LFNCGQL VPLGSY+
Sbjct: 422 QLCIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLVVPLGSYI 481

Query: 486 LNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAYKTR 545
           LNVD+VGKLYD EALREGR+  GKGITCTGAHCF GSFTILAA TLFGAL ML+LAY+TR
Sbjct: 482 LNVDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVMLLLAYRTR 541

Query: 546 EFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           ++YR DIYK Y+E+MWIPQ +MEFY LDN+KNIDD
Sbjct: 542 DYYRWDIYKNYKEDMWIPQAEMEFYRLDNRKNIDD 573

[Close]

BLAST of Spg032589 vs. NCBI nr
Match: XP_022970240.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 884.0 bits (2283), Expect = 6.9e-253
Identity = 433/578 (74.91%), Postives = 497/578 (85.99%), Query Frame = 0


Query: 1   MANERQDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNT 60
           M N+   E  GFIKQV+ GRWFSVFA  +MMIGNG+TY+F TYSKVIKTQFDYSQTQ+NT
Sbjct: 1   MGNKGGSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFL 120
           LGFAKDLGSNVG+ AGLLAEV P WV F++GA  NF+G+F+IWLSIT RI KP+FWQ+F+
Sbjct: 61  LGFAKDLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICIGTNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPS 180
            +C GTNSSN+ANTAIMVTSVRNFPDRRGIILGLLKG+VGIGGAI++QLYLAIYGH+DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPS 180

Query: 181 DLVLLFSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQV 240
           +LVLLF+WLPS +IL+LF S+RPI+IRKHP ELKVFY LLYVSI+LA+FILFLTI QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHAGYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPE 300
            FS AGY  GAS +V LL + +LIA REE LLY L KQ   PS  VS+V Q PP  +A  
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLSASA 300

Query: 301 NSMSTLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAID 360
           +S     E  E+ PSC  +I+NKP RGEDFTILQA+FSKDMALICLATFSGCGSSLAAID
Sbjct: 301 DS-----EIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAID 360

Query: 361 NLGQIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLIT 420
           NLGQ+GESLGYP +AI I VSWVS+FNFFGRV SGFISE LM KYK+PRPL F+FAFLIT
Sbjct: 361 NLGQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLIT 420

Query: 421 CIGQLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLG 480
           C+GQL IAYP PGSVY A++IIGFGFGAQNP+LFAVIS++FGL+HYSTLFNCGQL VPLG
Sbjct: 421 CVGQLFIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLG 480

Query: 481 SYLLNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAY 540
           SY+LNVDVVGKLYD EAL EG++  GKG+TCTGAHCF GSFTILAA TLFGA+ MLVLAY
Sbjct: 481 SYILNVDVVGKLYDMEALHEGKQIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAY 540

Query: 541 KTREFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           +TREFY+ D+YK + EE+WIPQT+MEFY LDNKK+I+D
Sbjct: 541 RTREFYKRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

[Close]

BLAST of Spg032589 vs. NCBI nr
Match: XP_004147308.1 (uncharacterized protein LOC101204150 [Cucumis sativus] >KAE8652748.1 hypothetical protein Csa_014134 [Cucumis sativus])

HSP 1 Score: 883.6 bits (2282), Expect = 9.0e-253
Identity = 431/573 (75.22%), Postives = 501/573 (87.43%), Query Frame = 0


Query: 8   EVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNTLGFAKDL 67
           E   FIKQV +GRWFSV+A  +MMIGNG+TYIFGTYSKV+K++FDYSQTQVN LGFAKDL
Sbjct: 4   EAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDL 63

Query: 68  GSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFLYICIGTN 127
           G+N G+ AGLL+E  P WV FMIGA  NF+GYF+IWLS+TRRI +P FWQ+FL +C G+N
Sbjct: 64  GNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSN 123

Query: 128 SSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPSDLVLLFS 187
           SSN++NTAIMVTS+RNFPDRRGIILGLLKG+VGIGGAI+TQ+ L  YG +DPS++VLLF+
Sbjct: 124 SSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVLLFA 183

Query: 188 WLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQVTFSHAGY 247
           W PS +ILL+  S+RPI IRKHPEELKVFYHLLYVSIVLA+FILFLT+++KQV FS + Y
Sbjct: 184 WFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQSAY 243

Query: 248 ASGASVIVGLLSLHLLIAVREEFLLYNLKKQ--NCDPSAIVSIVEQKPPLATAPENSMST 307
           ASGASV++ LL L LLIA REEFLLY LKKQ  N +PS  +SI++QK P +  P    ST
Sbjct: 244 ASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKP---FST 303

Query: 308 LEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAIDNLGQI 367
           LEE  E+SPSC S+I NKP RGEDFTILQAIFS DM LICLATF+GCGSSLAAIDNLGQI
Sbjct: 304 LEEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQI 363

Query: 368 GESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLITCIGQL 427
           GESLGYPP+AI IFVSWVS+FNFFGRV SGFISE +M+KYK+PRPL+FAFAFL+TCIGQL
Sbjct: 364 GESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQL 423

Query: 428 CIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLGSYLLN 487
           CIAYP PGS+Y+AS++IGFGFGAQNPLLFAVIS++FGLKHYS LFNCGQLAVPLGSY+LN
Sbjct: 424 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 483

Query: 488 VDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAYKTREF 547
           VD+VGKLYD EALREG++  G+GI C+GAHCF GSFTILAA TLFGAL MLVLAY+TRE+
Sbjct: 484 VDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREY 543

Query: 548 YRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           YR D+YK Y+E+MWIPQ +MEFY LDNKKNIDD
Sbjct: 544 YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573

[Close]

BLAST of Spg032589 vs. NCBI nr
Match: XP_022970365.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 882.5 bits (2279), Expect = 2.0e-252
Identity = 433/578 (74.91%), Postives = 497/578 (85.99%), Query Frame = 0


Query: 1   MANERQDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNT 60
           M N+   E  GFIKQV+ GRWFSVFA  +MMIGNG+TY+F TYSKVIKTQFDYSQTQ+NT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFL 120
           LGFAKDLGSN+G+ AGLLAEV P WV F+IGA  NF G+F+IWLSIT RI KP+FWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICIGTNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPS 180
            +C GTNSSN+ANTAIMVTSVRNFPDRRGIILGLLKG+VGIGGAI++Q YLAIYGH+DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 DLVLLFSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQV 240
           +LVLLF+WLPS +IL+LF S+RPI+IRKHP ELKVFY LLYVSI+LA+FILFLTI QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHAGYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPE 300
            FS AGY  GAS +V LL + +LIA REE LLY L KQ   PS  VS+V Q PP   A  
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300

Query: 301 NSMSTLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAID 360
           +S     E  E+SPSC  +I+NKP RGEDFTILQA+FSKDMALICLATFSGCGSSLAAID
Sbjct: 301 DS-----EIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAID 360

Query: 361 NLGQIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLIT 420
           NLGQ+GESLGYP +AI I VSWVS+FNFFGRV SGFISE LM KYK+PRPL F+FAFLIT
Sbjct: 361 NLGQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLIT 420

Query: 421 CIGQLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLG 480
           C+GQL IAYP PGSVY+A++IIGFGFGAQNP+LFAVIS++FGL+ YSTLFNCGQLAVPLG
Sbjct: 421 CVGQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLG 480

Query: 481 SYLLNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAY 540
           SY+LNVDVVGKLYD EALREG++  GKG+TCTGAHCF GSFTILAA TLFGA+ MLVLAY
Sbjct: 481 SYILNVDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAY 540

Query: 541 KTREFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           +TREFY+ D+YK + EE+WIPQT+MEFY LD+KK+I+D
Sbjct: 541 RTREFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

[Close]

BLAST of Spg032589 vs. NCBI nr
Match: KAG6583526.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583533.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583538.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583544.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 874.0 bits (2257), Expect = 7.1e-250
Identity = 430/578 (74.39%), Postives = 497/578 (85.99%), Query Frame = 0


Query: 1   MANERQDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNT 60
           M N+   E   FIKQV+ GRWFSVFA  +MMIGNG+TY+F TYSKVIKTQFDYSQTQ+NT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFL 120
           LGFAKDLGSN+G+ AGLLAEV P WV F+IGA  NF+G+F+IWLSIT RI KP+FWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICIGTNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPS 180
            +C+GTNSSN+ANTAIMVTSVRNFPDRRGIILGLLKG+VGIGGAII+QLYLAIYGH+DPS
Sbjct: 121 LVCLGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 DLVLLFSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQV 240
           +LVLLF+WLPS +IL+LF S+RPI+IRKHP+ELKVFY LLYVSI+LA+FILFLTI QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHAGYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPE 300
            FS AGY  GAS +V LL + +LIA REE LLY L KQ   PS  +S++ Q PP  T P 
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQTAAPSVALSMLHQNPP--TLPT 300

Query: 301 NSMSTLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAID 360
           ++ S   E  E+SPSC  +I+NKP RGEDFTILQA+FSKDMALICLATFSGCGSSLAAID
Sbjct: 301 SADS---EIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAID 360

Query: 361 NLGQIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLIT 420
           NLGQ+GESLGY  +AI I VSWVS+FNFFGRV SGFISE LM KYK+PRPL FAFAFLIT
Sbjct: 361 NLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLIT 420

Query: 421 CIGQLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLG 480
           C+GQL IAYP PGS+Y+A++IIGFGFGAQNP+LFAVIS++FGLK YSTLFNCGQLA P G
Sbjct: 421 CVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFG 480

Query: 481 SYLLNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAY 540
           SY+LNVDVVGKLYD EALRE ++  GKG+TCTGAHCF GSFT+LAA TLFGA+ M VLAY
Sbjct: 481 SYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAY 540

Query: 541 KTREFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           +TREFY+ D+YK + EE+WIPQT+MEFY LDNKKNI+D
Sbjct: 541 RTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Spg032589 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.3e-28
Identity = 136/534 (25.47%), Postives = 227/534 (42.51%), Query Frame = 0


Query: 20  RWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNTLGFAKDLGSNVGVFAGLLA 79
           +W  + A+  +    G+ + F  YS  +K+    SQ ++N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 80  EVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFLYICIGTNSSNFANTAIMVT 139
              PL V     A + F GY + WL IT  I  P +  +FL   +   S  + NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 140 SVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPSDLVLLF-SWLPSTVILLLF 199
            +R+FP+ R + L L   F GI  A+ +  + AI  +   S+L LL  S +P  V     
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAI--NPSSSNLYLLLNSLVPLVVSFAAL 222

Query: 200 YSV--RP-IKIRKHPEELKVFYHLLYVSIVLALFILF-LTITQKQVTFSHAGYASGASVI 259
           Y V  +P +      +  +   H+  +  VLA+   F L ++    + +   +     ++
Sbjct: 223 YPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVVLL 282

Query: 260 VGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPENSMSTLEEFGEVSP 319
           V  L   LL+  R+ FL     + N + S  V +   +        +S +  E  G    
Sbjct: 283 VFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEHMGTAKE 342

Query: 320 SCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAIDNLGQIGESLGYPPK 379
                  N    G++ +    I   +  L  +A F G    L   +NLGQI +SLG   +
Sbjct: 343 G------NTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG---Q 402

Query: 380 AISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLITCIGQLCIAYPS--P 439
             +  V+  S F+FFGR+ S    + +  ++++ R   FA A L T I    +A  S   
Sbjct: 403 NSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQ 462

Query: 440 GSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLGSYLLNVDVVGKL 499
            ++  A+ +IG   G       ++ SDLFG        N     +P+GS L    +   +
Sbjct: 463 TALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAASI 522

Query: 500 YDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAYKTREFY 547
           Y+  A  +    +   I C G  C+  +F      ++ G ++ L L  +T+  Y
Sbjct: 523 YEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

[Close]

BLAST of Spg032589 vs. ExPASy TrEMBL
Match: A0A1S3CMS0 (uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=4 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 7.2e-256
Identity = 442/575 (76.87%), Postives = 499/575 (86.78%), Query Frame = 0


Query: 6   QDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNTLGFAK 65
           + E   FIKQV+QGRWFSV+A  +MMIGNG+TYIFGTYSKV+KT+FDYSQTQVN LGFAK
Sbjct: 2   ESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFAK 61

Query: 66  DLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFLYICIG 125
           DLGSNVG+ AGLLAE  P WV F IGA  NF+ YF IWLS+TRRIP+PEFWQ+FL +C G
Sbjct: 62  DLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCFG 121

Query: 126 TNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPSDLVLL 185
           +NSSN+ANTAIMV S+RNFPDRRGIILGLLKG+VGIGGAI+TQ+YL IYG +DPSDLVLL
Sbjct: 122 SNSSNYANTAIMVISLRNFPDRRGIILGLLKGYVGIGGAILTQIYLGIYGPEDPSDLVLL 181

Query: 186 FSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQVTFSHA 245
           F+W PS +ILL+  S+RPI IRKHPEELKVFYHLLYVSI++A+FI FL+ITQKQVTFS A
Sbjct: 182 FAWFPSVLILLISTSIRPIHIRKHPEELKVFYHLLYVSIIIAIFIFFLSITQKQVTFSPA 241

Query: 246 GYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNC--DPSAIVSIVEQKPPLATAPENSM 305
            YASGA V+V LL L LLI  REEFLLY LKKQN   DPS  +SIV+QK    T    S 
Sbjct: 242 AYASGAYVVVALLCLPLLIVCREEFLLYKLKKQNLNRDPSVALSIVDQK---VTNCPKSF 301

Query: 306 STLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAIDNLG 365
           STLEE  E+SPSC S+I NKP RG DFT+LQAIFS DM LICLATFSGCGSSLAAIDNLG
Sbjct: 302 STLEEIAEISPSCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSSLAAIDNLG 361

Query: 366 QIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLITCIG 425
           QIGESLGYP +AISIFVSWVS+FNFFGRV SGFISE +++KYK+PRPL+FAFAFL+TC+G
Sbjct: 362 QIGESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAFAFLLTCLG 421

Query: 426 QLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLGSYL 485
           QLCIAYP PGSVY+AS IIGFGFGAQNPLLFAVIS++FGLKHYS LFNCGQL VPLGSY+
Sbjct: 422 QLCIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLVVPLGSYI 481

Query: 486 LNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAYKTR 545
           LNVD+VGKLYD EALREGR+  GKGITCTGAHCF GSFTILAA TLFGAL ML+LAY+TR
Sbjct: 482 LNVDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVMLLLAYRTR 541

Query: 546 EFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           ++YR DIYK Y+E+MWIPQ +MEFY LDN+KNIDD
Sbjct: 542 DYYRWDIYKNYKEDMWIPQAEMEFYRLDNRKNIDD 573

[Close]

BLAST of Spg032589 vs. ExPASy TrEMBL
Match: A0A6J1I2B3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469248 PE=4 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 3.3e-253
Identity = 433/578 (74.91%), Postives = 497/578 (85.99%), Query Frame = 0


Query: 1   MANERQDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNT 60
           M N+   E  GFIKQV+ GRWFSVFA  +MMIGNG+TY+F TYSKVIKTQFDYSQTQ+NT
Sbjct: 1   MGNKGGSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFL 120
           LGFAKDLGSNVG+ AGLLAEV P WV F++GA  NF+G+F+IWLSIT RI KP+FWQ+F+
Sbjct: 61  LGFAKDLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICIGTNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPS 180
            +C GTNSSN+ANTAIMVTSVRNFPDRRGIILGLLKG+VGIGGAI++QLYLAIYGH+DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPS 180

Query: 181 DLVLLFSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQV 240
           +LVLLF+WLPS +IL+LF S+RPI+IRKHP ELKVFY LLYVSI+LA+FILFLTI QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHAGYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPE 300
            FS AGY  GAS +V LL + +LIA REE LLY L KQ   PS  VS+V Q PP  +A  
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLSASA 300

Query: 301 NSMSTLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAID 360
           +S     E  E+ PSC  +I+NKP RGEDFTILQA+FSKDMALICLATFSGCGSSLAAID
Sbjct: 301 DS-----EIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAID 360

Query: 361 NLGQIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLIT 420
           NLGQ+GESLGYP +AI I VSWVS+FNFFGRV SGFISE LM KYK+PRPL F+FAFLIT
Sbjct: 361 NLGQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLIT 420

Query: 421 CIGQLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLG 480
           C+GQL IAYP PGSVY A++IIGFGFGAQNP+LFAVIS++FGL+HYSTLFNCGQL VPLG
Sbjct: 421 CVGQLFIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLG 480

Query: 481 SYLLNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAY 540
           SY+LNVDVVGKLYD EAL EG++  GKG+TCTGAHCF GSFTILAA TLFGA+ MLVLAY
Sbjct: 481 SYILNVDVVGKLYDMEALHEGKQIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAY 540

Query: 541 KTREFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           +TREFY+ D+YK + EE+WIPQT+MEFY LDNKK+I+D
Sbjct: 541 RTREFYKRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

[Close]

BLAST of Spg032589 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 9.7e-253
Identity = 433/578 (74.91%), Postives = 497/578 (85.99%), Query Frame = 0


Query: 1   MANERQDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNT 60
           M N+   E  GFIKQV+ GRWFSVFA  +MMIGNG+TY+F TYSKVIKTQFDYSQTQ+NT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFL 120
           LGFAKDLGSN+G+ AGLLAEV P WV F+IGA  NF G+F+IWLSIT RI KP+FWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICIGTNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPS 180
            +C GTNSSN+ANTAIMVTSVRNFPDRRGIILGLLKG+VGIGGAI++Q YLAIYGH+DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 DLVLLFSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQV 240
           +LVLLF+WLPS +IL+LF S+RPI+IRKHP ELKVFY LLYVSI+LA+FILFLTI QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHAGYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPE 300
            FS AGY  GAS +V LL + +LIA REE LLY L KQ   PS  VS+V Q PP   A  
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQTAGPSVTVSMVHQNPPTLPASA 300

Query: 301 NSMSTLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAID 360
           +S     E  E+SPSC  +I+NKP RGEDFTILQA+FSKDMALICLATFSGCGSSLAAID
Sbjct: 301 DS-----EIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAID 360

Query: 361 NLGQIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLIT 420
           NLGQ+GESLGYP +AI I VSWVS+FNFFGRV SGFISE LM KYK+PRPL F+FAFLIT
Sbjct: 361 NLGQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLIT 420

Query: 421 CIGQLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLG 480
           C+GQL IAYP PGSVY+A++IIGFGFGAQNP+LFAVIS++FGL+ YSTLFNCGQLAVPLG
Sbjct: 421 CVGQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLG 480

Query: 481 SYLLNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAY 540
           SY+LNVDVVGKLYD EALREG++  GKG+TCTGAHCF GSFTILAA TLFGA+ MLVLAY
Sbjct: 481 SYILNVDVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAY 540

Query: 541 KTREFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           +TREFY+ D+YK + EE+WIPQT+MEFY LD+KK+I+D
Sbjct: 541 RTREFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

[Close]

BLAST of Spg032589 vs. ExPASy TrEMBL
Match: A0A6J1HLT1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464780 PE=4 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.3e-249
Identity = 429/578 (74.22%), Postives = 496/578 (85.81%), Query Frame = 0


Query: 1   MANERQDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNT 60
           M N+   E   FIKQV+ GRWFSVFA  +MMIGNG+TY+F TYSKVIKTQFDYSQTQ+NT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFL 120
           LGFAKDLGSN+G+ AGLLAEV P WV F+IGA  NF+G+F+IWLSIT RI KP+FWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICIGTNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPS 180
            +C GTNSSN+ANTAIMVTSVRNFPDRRGIILGLLKG+VGIGGAII+QLYLAIYGH+DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 DLVLLFSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQV 240
           +LVLLF+WLPS +IL+LF S+RPI+IRKHP+ELKVFY LLYVSI+LA+FILFLTI QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHAGYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPE 300
            FS AGY  GAS +V LL + +LIA REE LLY L K+   PS  +S++ Q PP  T P 
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKETAAPSVALSMLHQNPP--TLPT 300

Query: 301 NSMSTLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAID 360
           ++ S   E  E+SPSC  +I+NKP RGEDFTILQA+FSKDMALICLATFSGCGSSLAAID
Sbjct: 301 SADS---EIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAID 360

Query: 361 NLGQIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLIT 420
           NLGQ+GESLGY  +AI I VSWVS+FNFFGRV SGFISE LM KYK+PRPL FAFAFLIT
Sbjct: 361 NLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLIT 420

Query: 421 CIGQLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLG 480
           C+GQL IAYP PGS+Y+A++IIGFGFGAQNP+LFAVIS++FGLK YSTLFNCGQLA P G
Sbjct: 421 CVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFG 480

Query: 481 SYLLNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAY 540
           SY+LNVDVVGKLYD EALRE ++  GKG+TCTGAHCF GSFT+LAA TLFGA+ M VLAY
Sbjct: 481 SYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAY 540

Query: 541 KTREFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           +TREFY+ D+YK + EE+WIPQT+MEFY LDNKKNI+D
Sbjct: 541 RTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Spg032589 vs. ExPASy TrEMBL
Match: A0A6J1HLS1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464770 PE=4 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.7e-249
Identity = 429/578 (74.22%), Postives = 496/578 (85.81%), Query Frame = 0


Query: 1   MANERQDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNT 60
           M N+   E   FIKQV+ GRWFSVFA  +MMIGNG+TY+F TYSKVIKTQFDYSQTQ+NT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFL 120
           LGFAKDLGSN+G+ AGLLAEV P WV F+IGA  NF+G+F+IWLSIT RI KP+FWQ+F+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICIGTNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPS 180
            +C GTNSSN+ANTAIMVTSVRNFPDRRGIILGLLKG+VGIGGAII+QLYLAIYGH+DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 DLVLLFSWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQV 240
           +LVLLF+WLPS +IL+LF S+RPI+IRKHP+ELKVFY LLYVSI+LA+FILFLTI QKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHAGYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPE 300
            FS AGY  GAS +V LL + +LIA REE LLY L K+   PS  +S++ Q PP  T P 
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKETAAPSVALSMLHQNPP--TLPT 300

Query: 301 NSMSTLEEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAID 360
           ++ S   E  E+SPSC  +I+NKP RGEDFTILQA+FSKDMALICLATFSGCGSSLAAID
Sbjct: 301 SADS---EIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAID 360

Query: 361 NLGQIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLIT 420
           NLGQ+GESLGY  +AI I VSWVS+FNFFGRV SGFISE LM KYK+PRPL FAFAFLIT
Sbjct: 361 NLGQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLIT 420

Query: 421 CIGQLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLG 480
           C+GQL IAYP PGS+Y+A++IIGFGFGAQNP+LFAVIS++FGLK YSTLFNCGQLA P G
Sbjct: 421 CVGQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFG 480

Query: 481 SYLLNVDVVGKLYDREALREGRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAY 540
           SY+LNVDVVGKLYD EALRE ++  GKG+TCTGAHCF GSFT+LAA TLFGA+ M VLAY
Sbjct: 481 SYILNVDVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAY 540

Query: 541 KTREFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNIDD 579
           +TREFY+ D+YK + EE+WIPQT+MEFY LDNKKNI+D
Sbjct: 541 QTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Spg032589 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 607.8 bits (1566), Expect = 8.9e-174
Identity = 312/576 (54.17%), Postives = 410/576 (71.18%), Query Frame = 0


Query: 7   DEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNTLGFAKD 66
           DE + F+     GRWF VFASFL+M   G+TY+FGTYSK IK+   Y QT +N LGF KD
Sbjct: 4   DETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKD 63

Query: 67  LGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFLYICIGT 126
           LG+NVGV +GL+AEV P W    IG+ +NF GYFMIWL++T ++ KP+ WQ+ LYICIG 
Sbjct: 64  LGANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGA 123

Query: 127 NSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPSDLVLLF 186
           NS NFANT  +VT V+NFP+ RG++LGLLKG+VG+ GAI TQLY AIYGHD  S L+LL 
Sbjct: 124 NSQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKS-LILLI 183

Query: 187 SWLPSTVILLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQVTFSHAG 246
           +WLP+ V L+  Y +R  K+ +   EL VFY  LY+SI LALF++ + I +KQV FS A 
Sbjct: 184 AWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAA 243

Query: 247 YASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPENSMSTL 306
           YA+ A++   LL + L ++V++E  ++N+ K   +  + V + + K  L    + +    
Sbjct: 244 YAASATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVN 303

Query: 307 EEFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAIDNLGQIG 366
            E  E + SCFS +F+ PPRGED+TILQA+ S DM ++ +ATF G GSSL A+DNLGQIG
Sbjct: 304 GEEKE-TKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIG 363

Query: 367 ESLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLITCIGQLC 426
           ESLGYP   +S FVS VS++N+FGRV SGF+SE L+ KYK+PRPL+     L++C G L 
Sbjct: 364 ESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLL 423

Query: 427 IAYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLGSYLLNV 486
           IA+P PGSVY+AS+++GF FGAQ PLLFA+IS+LFGLK+YSTLFNCGQLA PLGSY+LNV
Sbjct: 424 IAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNV 483

Query: 487 DVVGKLYDREALRE-----GRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAYK 546
            V G LYD+EAL++       R   K +TC G+ C+   F ILAA T FGAL  L LA +
Sbjct: 484 RVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIR 543

Query: 547 TREFYRGDIYKKYREEMWIPQTKMEFYCLDNKKNID 578
           TREFY+GDIYKK+RE    P+++ E    D++K ++
Sbjct: 544 TREFYKGDIYKKFRES---PESESEL-VPDSRKAVE 573

[Close]

BLAST of Spg032589 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 550.1 bits (1416), Expect = 2.2e-156
Identity = 279/562 (49.64%), Postives = 387/562 (68.86%), Query Frame = 0


Query: 15  QVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNTLGFAKDLGSNVGVF 74
           Q+L GRWF  F S L+M   G+TY+FG YS  IK    Y QT +N L F KDLG+NVGV 
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 75  AGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFLYICIGTNSSNFANT 134
           AGLL EV P W   +IGAILNF GYFMIWL++T RI KP+ W + LYIC+G NS +FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 135 AIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPSDLVLLFSWLPSTVI 194
             +VT V+NFP+ RG++LG+LKG+VG+ GAIITQLY A YG +D  +L+L+  WLP+ V 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 195 LLLFYSVRPIKIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQVTFSHAGYASGASVI 254
                ++R +K+++   ELKVFY+ LY+S+ LA F++ + I  K   F+ + +   A+V+
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 255 VGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAP------ENSMSTLEE 314
           + LL L +++ + EE  L+  K+   +  A +++V +KP L ++       E S   +E+
Sbjct: 256 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEK 315

Query: 315 FGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAIDNLGQIGES 374
               +PSC++ +FN P RG+D+TILQA+FS DM ++ LAT  G G +L AIDNLGQIG S
Sbjct: 316 V--KTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNS 375

Query: 375 LGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLITCIGQLCIA 434
           LGYP +++S FVS VS++N++GRV SG +SE  ++KYK PRPL+     L++C G L IA
Sbjct: 376 LGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIA 435

Query: 435 YPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLGSYLLNVDV 494
           +  PG +Y+AS+IIGF FGAQ PLLFA+IS++FGLK+YSTL+N G +A P+GSYLLNV V
Sbjct: 436 FNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRV 495

Query: 495 VGKLYDREALREGR-----RTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAYKTR 554
            G LYD EA ++ +     R  G+ + C G  CF  SF I+AA TLFG L  +VL  +T+
Sbjct: 496 AGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTK 555

Query: 555 EFYRGDIYKKYREEMWIPQTKM 566
           +FY+ DIYKK+RE+    + +M
Sbjct: 556 KFYKSDIYKKFREKALAAEMEM 574

[Close]

BLAST of Spg032589 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 7.2e-83
Identity = 188/586 (32.08%), Postives = 308/586 (52.56%), Query Frame = 0


Query: 1   MANERQDEVLGFIKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNT 60
           MA+  +++ + FI      RW    A+  +    G  Y+FG+ S VIK+  +Y+Q +++ 
Sbjct: 1   MASTTREKFVSFI----NNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSR 60

Query: 61  LGFAKDLGSNVGVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFL 120
           LG AKDLG +VG  AG L+E+ PLW   ++GA+ N  GY  +WL +T R P    W + +
Sbjct: 61  LGVAKDLGDSVGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCV 120

Query: 121 YICIGTNSSNFANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPS 180
            I +G N   + NT  +V+ V+NFP  RG ++G+LKGF G+GGAII+Q+Y  I+   +P+
Sbjct: 121 LIFVGNNGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHS-SNPA 180

Query: 181 DLVLLFSWLPSTVILLLFYSVRPIKIRK--HPEELKVFYHLLYVSIVLALFILFLTITQK 240
            L+L+ +  P+ V++ L + +RP+   K   P +   F  +  V ++LA +++ + + Q 
Sbjct: 181 SLILMVAVTPAVVVVCLMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQD 240

Query: 241 QVTFSHAGYASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATA 300
            V  SH        V+  +L + +L+ +   F     +  +     +V   E + P    
Sbjct: 241 LVVVSHNVITVFTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQT 300

Query: 301 PENSMSTLEEF--GEVSPSCFSDIFNK------------------------PPRGEDFTI 360
           P+  +S +E+    +V     S+   +                        P RGEDFT+
Sbjct: 301 PDLILSEVEDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTL 360

Query: 361 LQAIFSKDMALICLATFSGCGSSLAAIDNLGQIGESLGYPPKAISIFVSWVSVFNFFGRV 420
            QA+   D  LI  +   G GS L  IDNLGQ+ +SLGY      + VS +S++NF GR+
Sbjct: 361 TQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRI 420

Query: 421 CSGFISEALMVKYKMPRPLLFAFAFLITCIGQLCIAYPSPGSVYMASLIIGFGFGAQNPL 480
             G+ SE ++  Y  PRP+  A A LI  +G +  AY  PG++Y+ +L+IG G+GA   +
Sbjct: 421 GGGYFSELVVRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAI 480

Query: 481 LFAVISDLFGLKHYSTLFNCGQLAVPLGSYLLNVDVVGKLYDREALREGRRTM---GKGI 540
           + A  S+LFGLK +  L+N   LA P GS + +  +   +YDREA R+   ++      +
Sbjct: 481 VPATASELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDAL 540

Query: 541 TCTGAHCFSGSFTILAAFTLFGALTMLVLAYKTREFYRGDIYKKYR 556
            C G+ CF  +  I++ F +   +  ++L  +T+  Y   +Y K R
Sbjct: 541 RCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVYT-HLYGKTR 578

[Close]

BLAST of Spg032589 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 299.7 bits (766), Expect = 5.2e-81
Identity = 188/579 (32.47%), Postives = 306/579 (52.85%), Query Frame = 0


Query: 13  IKQVLQGRWFSVFASFLMMIGNGSTYIFGTYSKVIKTQFDYSQTQVNTLGFAKDLGSNVG 72
           +K  +  RW    A+  +    G  Y+FG+ S VIK+  +Y+Q Q++ LG AKDLG +VG
Sbjct: 9   VKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVG 68

Query: 73  VFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFLYICIGTNSSNFA 132
             AG L+E+ PLW   ++G++ N  GY  +WL +T R P    W + + I +G N   + 
Sbjct: 69  FLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYF 128

Query: 133 NTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPSDLVLLFSWLPST 192
           NTA +V+ V+NFP  RG ++G+LKGF G+GGAI++Q+Y  I+  D  S L+ + +  PS 
Sbjct: 129 NTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRAS-LIFMVAVAPSV 188

Query: 193 VILLLFYSVRPIKIRK--HPEELKVFYHLLYVSIVLALFILFLTITQKQVTFSHAGYASG 252
           V++ L + +RP+   +     +   F  +  V I+LA +++ + + +  +  SH+   + 
Sbjct: 189 VVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAF 248

Query: 253 ASVIVGLLSLHLLIAVR--------------EEFLLYNLKKQ--------NCDPSAIVSI 312
             V+  +L + + I +               EE LL + + Q        +  P  I S 
Sbjct: 249 TVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSE 308

Query: 313 VEQKPP----LATAPENSMSTLEEFGEVSPSCFSDIF-----NKPPRGEDFTILQAIFSK 372
           VE + P    L  A E      +   ++  +             P RGEDFT+ QA+   
Sbjct: 309 VEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKA 368

Query: 373 DMALICLATFSGCGSSLAAIDNLGQIGESLGYPPKAISIFVSWVSVFNFFGRVCSGFISE 432
           D  LI  +   G GS L  IDNLGQ+ +SLGY      +FVS +S++NF GR+  G+ SE
Sbjct: 369 DFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGY--DNTHVFVSMISIWNFLGRIGGGYFSE 428

Query: 433 ALMVKYKMPRPLLFAFAFLITCIGQLCIAYPSPGSVYMASLIIGFGFGAQNPLLFAVISD 492
            ++  Y  PRP+  A A L+  +G +  AY  PG++++ +L+IG G+GA   ++ A  S+
Sbjct: 429 LIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASE 488

Query: 493 LFGLKHYSTLFNCGQLAVPLGSYLLNVDVVGKLYDREALREGRRTM---GKGITCTGAHC 552
           LFGLK +  L+N   LA P GS + +  +   +YDREA R+ + ++      + C G+ C
Sbjct: 489 LFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSIC 548

Query: 553 FSGSFTILAAFTLFGALTMLVLAYKTREFYRGDIYKKYR 556
           +  +  I++ F L  A   ++L  +T+  Y  ++Y K R
Sbjct: 549 YFLTSLIMSGFCLIAAALSMILVQRTKPVYT-NLYGKTR 583

[Close]

BLAST of Spg032589 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 293.1 bits (749), Expect = 4.8e-79
Identity = 176/540 (32.59%), Postives = 296/540 (54.81%), Query Frame = 0


Query: 13  IKQVLQGRWFSVFASFLMMIGNGSTYIF-GTYSKVIKTQFDYSQTQVNTLGFAKDLGSNV 72
           ++ ++  RW     +  +    G  Y+F G+ S  IKT   Y+Q Q+  LG AK+LG  +
Sbjct: 9   LELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAI 68

Query: 73  GVFAGLLAEVAPLWVQFMIGAILNFSGYFMIWLSITRRIPKPEFWQLFLYICIGTNSSNF 132
           G  +G L+EV+P WV  ++GA  N  GY ++WL +T ++P    W LF+ I +GTN   +
Sbjct: 69  GFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETY 128

Query: 133 ANTAIMVTSVRNFPDRRGIILGLLKGFVGIGGAIITQLYLAIYGHDDPSDLVLLFSWLPS 192
            NTA +V+ + NFP+ RG ++G+LKGF G+ GAI+TQ+YL ++     S ++L+ +  P 
Sbjct: 129 YNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPP 188

Query: 193 TVILLLFYSVRPI----KIRKHPEELKVFYHLLYVSIVLALFILFLTITQKQVTFSHAGY 252
            V+L L + VRP+    +     ++L+ F  +    +VLA+++L L + Q     +    
Sbjct: 189 VVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTII 248

Query: 253 ASGASVIVGLLSLHLLIAVREEFLLYNLKKQNCDPSAIVSIVEQKPPLATAPENSMSTLE 312
            +  +++V  + + +L+     F+  N           V+ V+ +   +   ++   TL 
Sbjct: 249 TTSGAILVIFMVVPVLVPFSSVFISGN----------NVTSVKPEEGTSNVDQHEARTLI 308

Query: 313 EFGEVSPSCFSDIFNKPPRGEDFTILQAIFSKDMALICLATFSGCGSSLAAIDNLGQIGE 372
           E  +  P    +    P  GEDFT+LQA+   D  LI ++   G GS +  IDNLGQI  
Sbjct: 309 ERSDRPP----EKKRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICY 368

Query: 373 SLGYPPKAISIFVSWVSVFNFFGRVCSGFISEALMVKYKMPRPLLFAFAFLITCIGQLCI 432
           SLGY      IFVS +S+ NF GRV  G+ SE ++ K  +PR L  +    I  +G +  
Sbjct: 369 SLGY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYY 428

Query: 433 AYPSPGSVYMASLIIGFGFGAQNPLLFAVISDLFGLKHYSTLFNCGQLAVPLGSYLLNVD 492
           A   PG +Y+ +++IG G+GA   +  A +SD+FGLK + +L+N    A+P+GS++ +  
Sbjct: 429 AIDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGV 488

Query: 493 VVGKLYDREALRE-GRRTMGKGITCTGAHCFSGSFTILAAFTLFGALTMLVLAYKTREFY 547
           +   +YD  A ++ G  T  + + CTG+ C+S + ++++   L   +  L + Y+TR+FY
Sbjct: 489 IASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

The following BLAST results are available for this feature:

BLAST of Spg032589 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008464773.1	1.5e-255	76.87	PREDICTED: uncharacterized protein LOC103502580 [Cucumis melo]	[more]
XP_022970240.1	6.9e-253	74.91	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima]	[more]
XP_004147308.1	9.0e-253	75.22	uncharacterized protein LOC101204150 [Cucumis sativus] >KAE8652748.1 hypothetica...	[more]
XP_022970365.1	2.0e-252	74.91	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 prote...	[more]
KAG6583526.1	7.1e-250	74.39	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of Spg032589 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	1.3e-28	25.47	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]

BLAST of Spg032589 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CMS0	7.2e-256	76.87	uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=...	[more]
A0A6J1I2B3	3.3e-253	74.91	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469...	[more]
A0A6J1I5D7	9.7e-253	74.91	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469...	[more]
A0A6J1HLT1	1.3e-249	74.22	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLS1	1.7e-249	74.22	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of Spg032589 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28120.1	8.9e-174	54.17	Major facilitator superfamily protein	[more]
AT2G39210.1	2.2e-156	49.64	Major facilitator superfamily protein	[more]
AT5G14120.1	7.2e-83	32.08	Major facilitator superfamily protein	[more]
AT3G01930.2	5.2e-81	32.47	Major facilitator superfamily protein	[more]
AT5G50630.1	4.8e-79	32.59	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.20.1720.10	Multidrug resistance protein D	coord: 1..200 e-value: 1.3E-6 score: 29.4
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 4..558
None	No IPR available	PANTHER	PTHR21576:SF29	PROTEIN NUCLEAR FUSION DEFECTIVE 4	coord: 4..558
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 20..265 e-value: 7.4E-77 score: 258.2
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 340..546 e-value: 1.6E-9 score: 39.5
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 17..544

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg032589.1	Spg032589.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity