Spg032482 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg032482
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionReverse transcriptase
Locationscaffold2: 29488343 .. 29490409 (+)
RNA-Seq ExpressionSpg032482
SyntenySpg032482
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGATTATCACTGCATATCTGTAAACTTGAGACTTCGCTTTGACTATTTGCTCTTGTCAGAAATTATGGGAAGTTGGATTAAAGTTGTTGTTGTTCATCGTTGAAGAAAATGGCAAAGCTTTGTGGTTTCATTTTTGGATTAAAGTTGTTGGAATTTATTAATTTAAAAGAAGAAAGAAAAGAAAGGAAATAAAGAAGAAAAAGAAAAGAGAAGAAAATATGAAAATGAAAAGAAAAAAAAAAGAAGAAAGGAAATGGGGAAAATGTTGTCGGTGCTAGTGTTTTAAGAAAAACAAACCCACCGCCAAGGATTCATTTCAGCCATTTTCTCCATCATTTCCATTAGATTTTCAGAGCATTTCGAGGGAGAGAGTGAAGTAAGAGGAAGAAGAAGTTTGTTGCAGGTTTGAGGTTGAAGAAGAAAGGAAAAAGCTCAAGCAAAAGCAACCATGGCAGAATCTGGGTTTAACCTGCCTATCTCTGGTTTTTAATTTGTTTTGAGCTACACAAGAAGAACTAAGAGGTGAGGCTTGACTTTCATGAAAGTTAATCCATTTTCTAACAAATTGCATGAAGGAAGCTTGAGCTAAATAGGATGTTAAGTTAATGGTTTTTGAAGAGGAAAACAGAGCACAAAATTTTCGTGCAAATCAGAAGGATCTCTGGTTTTTCGAACATTCTGGGTTGTACAGGTTGAATTAGAAGCTCAATTTCGTATGGTTGGAAAGCTTATTCAATTTTCCTATAACTTTCATGAAGAATTCATGAGCAAAAAATTGACTAGAAGTAGGTTTAATTTCAGGAGAAAAGTAGAGGGTAGCAGAGAAGCACGAATCTGGATTTTCTGTGTTAGGGGGATTTCTGGACCATTCCATTGGGAATCTCACTTTCATTCCTTCAGATAAGTTGTTGAGGGTTCGAAAATGAAGAGTTTGATATATAGTACATTAATTTTGGTCACTTATTAAGGAAGTTATGAGTGTTTGAAGTCTGGAAAATCTAGAAAAAAAATGTGTGAAGTGCGATTTTATTCTAGAGTTTAGGATTCATGAGCATGGAAAGACAAGACGATTTATAGTTCTGATGCTCGGTTTTGGTTTGTTTGACAGGCCAAGAGAAAATAGGTTGAGTTTACAGGCCAGGGAACTAGCCAAGACTGTGAGTGACAAAACAAATTTCGAAAATGATTTCTAAATTGTTGTTTATGATTAGATGTTTTACATGATTTTTATGAATGGTTTCAAGCATAAGTTTTGAGCTTAGATTTTCTAATGAGATTTACATACGAGCACGTGATTATTGACTTTCTGAAAAGTAGTTGAAACGATATCATTTAAAGTAAAGTATGTATTAGCAAGTACTGTTAAAAGATTTTCAGTTTTTCACAAGCAAGCTGACTAAGCTTGAGTTTTACGAAAGATAAAGATAAGCATGCATGTTTTAATGTTTTCATGTGGTTTTCTGAGACTTTTATTGACTACATGACTGAGATATTGAGCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTTTCGTGAGTGCTAGGCGGGCCCCACTACGACAAAGACGATGGGAGTGCTGGGCAGGCCCCACTACATCGTAGAGCTAGTAAACGTTGGTTGTACTGGGCGTGCCCTACACAACGTTGACTTGTCATGTTAGTTAAAATGTTTTATATACTTGATATGCCTTGCTAGATTTTAATGACATACTTGCGAATTATTTGAGAAAGCTTTTATCGTTACACGTTTATGTTTAACACTCTCATTGAACAGATTTATACGTTTAAAGGTTTATCACGTGTTCTAATTTGAGGATTTAAAGTTTTAAATTTAGTCACTCACTGGGCTTCGTAGCTCACTCTTTTAAAATGTCTTCCCACTTTTCAGGTAGAGATCGAGCTCCCGGTGCCTAATACACTGCCAACGTCTGCTGAAGGCTCCAAGTTGACTTCCAGTACGTGGGTGGAGTTGTACATCGAGTCTTTTTGTATTGTAAATGTCTTTGTAAGGATTGGATAG

mRNA sequence

AAGGATTATCACTGCATATCTGTAAACTTGAGACTTCGCTTTGACTATTTGCTCTTGTCAGAAATTATGGGAAGTTGGATTAAAGTTGTTGTTGAATTAGAAGCTCAATTTCGTATGGTTGGAAAGCTTATTCAATTTTCCTATAACTTTCATGAAGAATTCATGAGCAAAAAATTGACTAGAAGTAGGCCAAGAGAAAATAGGTTGAGTTTACAGGCCAGGGAACTAGCCAAGACTCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTTTCGTAGAGATCGAGCTCCCGGTGCCTAATACACTGCCAACGTCTGCTGAAGGCTCCAAGTTGACTTCCAGTACGTGGGTGGAGTTGTACATCGAGTCTTTTTGTATTGTAAATGTCTTTGTAAGGATTGGATAG

Coding sequence (CDS)

AAGGATTATCACTGCATATCTGTAAACTTGAGACTTCGCTTTGACTATTTGCTCTTGTCAGAAATTATGGGAAGTTGGATTAAAGTTGTTGTTGAATTAGAAGCTCAATTTCGTATGGTTGGAAAGCTTATTCAATTTTCCTATAACTTTCATGAAGAATTCATGAGCAAAAAATTGACTAGAAGTAGGCCAAGAGAAAATAGGTTGAGTTTACAGGCCAGGGAACTAGCCAAGACTCCCGAGGCTATATGGTACCGTGTGCACACAGGTTATATTCCGTTGTTGACGTTGAGTGTACTCCGTGACAACGATGCTTTCGTAGAGATCGAGCTCCCGGTGCCTAATACACTGCCAACGTCTGCTGAAGGCTCCAAGTTGACTTCCAGTACGTGGGTGGAGTTGTACATCGAGTCTTTTTGTATTGTAAATGTCTTTGTAAGGATTGGATAG

Protein sequence

KDYHCISVNLRLRFDYLLLSEIMGSWIKVVVELEAQFRMVGKLIQFSYNFHEEFMSKKLTRSRPRENRLSLQARELAKTPEAIWYRVHTGYIPLLTLSVLRDNDAFVEIELPVPNTLPTSAEGSKLTSSTWVELYIESFCIVNVFVRIG
Homology
[Close]
BLAST of Spg032482 vs. NCBI nr
Match: KAA0037514.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 87.4 bits (215), Expect = 1.1e-13
Identity = 57/108 (52.78%), Postives = 63/108 (58.33%), Query Frame = 0 

Query: 31  VELEAQFRMVGKLIQFSYNFHEEFMSKKLTRSRPRENRLSLQARELAKTPEAIWYRVHTG 90
           VE EA+   VGKLIQ+   F          +  PR NR SLQ REL KT   +       
Sbjct: 883 VESEARLGRVGKLIQYPTTF---------MKILPRGNRPSLQTRELDKTVIFL------- 942

Query: 91  YIPLLTLSVLRDNDAFVEIELPVPNTLPTSAEGSKLTSSTWVELYIES 139
              L TLSVLRDNDA VEIELPVP+TLPTS E     SSTW+ELY ES
Sbjct: 943 ---LSTLSVLRDNDAVVEIELPVPDTLPTSVESFGSNSSTWLELYFES 971

[Close]
BLAST of Spg032482 vs. NCBI nr
Match: TYK01989.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 87.4 bits (215), Expect = 1.1e-13
Identity = 57/108 (52.78%), Postives = 63/108 (58.33%), Query Frame = 0 

Query: 31  VELEAQFRMVGKLIQFSYNFHEEFMSKKLTRSRPRENRLSLQARELAKTPEAIWYRVHTG 90
           VE EA+   VGKLIQ+   F          +  PR NR SLQ REL KT   +       
Sbjct: 883 VESEARLGRVGKLIQYPTTF---------MKILPRGNRPSLQTRELDKTVIFL------- 942

Query: 91  YIPLLTLSVLRDNDAFVEIELPVPNTLPTSAEGSKLTSSTWVELYIES 139
              L TLSVLRDNDA VEIELPVP+TLPTS E     SSTW+ELY ES
Sbjct: 943 ---LSTLSVLRDNDAVVEIELPVPDTLPTSVESFGSNSSTWLELYFES 971

[Close]
BLAST of Spg032482 vs. NCBI nr
Match: TYK01618.1 (uncharacterized protein E5676_scaffold451G002250 [Cucumis melo var. makuwa])

HSP 1 Score: 83.2 bits (204), Expect = 2.1e-12
Identity = 49/75 (65.33%), Postives = 50/75 (66.67%), Query Frame = 0 

Query: 64  PRENRLSLQARELAKTPEAIWYRVHTGYIPLLTLSVLRDNDAFVEIELPVPNTLPTSAEG 123
           PRENR SLQ  EL KT             PL TLSVLRDNDA VEIEL VPNTLPTSAE 
Sbjct: 76  PRENRPSLQTGELDKTV----------IFPLSTLSVLRDNDAVVEIELLVPNTLPTSAES 135

Query: 124 SKLTSSTWVELYIES 139
           S   SSTW+ELY ES
Sbjct: 136 SISNSSTWLELYFES 140

[Close]
BLAST of Spg032482 vs. NCBI nr
Match: KAA0057197.1 (gag/pol protein [Cucumis melo var. makuwa])

HSP 1 Score: 82.8 bits (203), Expect = 2.7e-12
Identity = 50/86 (58.14%), Postives = 57/86 (66.28%), Query Frame = 0 

Query: 53  EFMSKKLTRSRPRENRLSLQARELAKTPEAIWYRVHTGYIPLLTLSVLRDNDAFVEIELP 112
           +F + K  R +PRENR SLQ  EL KT             PL TLSVLRDNDA VEIEL 
Sbjct: 366 DFQTDKDAR-KPRENRPSLQTGELDKTV----------IFPLSTLSVLRDNDAIVEIELL 425

Query: 113 VPNTLPTSAEGSKLTSSTWVELYIES 139
           VP+TLPTSAE S+  S+TW+ELY ES
Sbjct: 426 VPDTLPTSAESSESNSNTWLELYFES 440

[Close]
BLAST of Spg032482 vs. NCBI nr
Match: TYK08952.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 82.8 bits (203), Expect = 2.7e-12
Identity = 54/95 (56.84%), Postives = 63/95 (66.32%), Query Frame = 0 

Query: 64   PRENRLSLQARELAKT------PEAIWYR----VHTGYI-PLLTLSVLRDNDAFVEIELP 123
            PRENRLSLQ  EL KT       E +  R    V T  I PL TLSVLRDN+A VEIELP
Sbjct: 1265 PRENRLSLQTGELDKTLSIDYMTEILSLRLDGTVCTQVIFPLSTLSVLRDNNAVVEIELP 1324

Query: 124  VPNTLPTSAEGSKLTSSTWVELYIESFCIVNVFVR 148
            VP+TLPTSAE  +  SSTW+ELY+ES  +  +F +
Sbjct: 1325 VPDTLPTSAEIFESNSSTWLELYLESVHVEMLFYK 1359

[Close]
BLAST of Spg032482 vs. ExPASy TrEMBL
Match: A0A5D3BV68 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G001010 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 5.4e-14
Identity = 57/108 (52.78%), Postives = 63/108 (58.33%), Query Frame = 0 

Query: 31  VELEAQFRMVGKLIQFSYNFHEEFMSKKLTRSRPRENRLSLQARELAKTPEAIWYRVHTG 90
           VE EA+   VGKLIQ+   F          +  PR NR SLQ REL KT   +       
Sbjct: 883 VESEARLGRVGKLIQYPTTF---------MKILPRGNRPSLQTRELDKTVIFL------- 942

Query: 91  YIPLLTLSVLRDNDAFVEIELPVPNTLPTSAEGSKLTSSTWVELYIES 139
              L TLSVLRDNDA VEIELPVP+TLPTS E     SSTW+ELY ES
Sbjct: 943 ---LSTLSVLRDNDAVVEIELPVPDTLPTSVESFGSNSSTWLELYFES 971

[Close]
BLAST of Spg032482 vs. ExPASy TrEMBL
Match: A0A5A7T223 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold277G001250 PE=4 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 5.4e-14
Identity = 57/108 (52.78%), Postives = 63/108 (58.33%), Query Frame = 0 

Query: 31  VELEAQFRMVGKLIQFSYNFHEEFMSKKLTRSRPRENRLSLQARELAKTPEAIWYRVHTG 90
           VE EA+   VGKLIQ+   F          +  PR NR SLQ REL KT   +       
Sbjct: 883 VESEARLGRVGKLIQYPTTF---------MKILPRGNRPSLQTRELDKTVIFL------- 942

Query: 91  YIPLLTLSVLRDNDAFVEIELPVPNTLPTSAEGSKLTSSTWVELYIES 139
              L TLSVLRDNDA VEIELPVP+TLPTS E     SSTW+ELY ES
Sbjct: 943 ---LSTLSVLRDNDAVVEIELPVPDTLPTSVESFGSNSSTWLELYFES 971

[Close]
BLAST of Spg032482 vs. ExPASy TrEMBL
Match: A0A5D3BRI2 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G002250 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.0e-12
Identity = 49/75 (65.33%), Postives = 50/75 (66.67%), Query Frame = 0 

Query: 64  PRENRLSLQARELAKTPEAIWYRVHTGYIPLLTLSVLRDNDAFVEIELPVPNTLPTSAEG 123
           PRENR SLQ  EL KT             PL TLSVLRDNDA VEIEL VPNTLPTSAE 
Sbjct: 76  PRENRPSLQTGELDKTV----------IFPLSTLSVLRDNDAVVEIELLVPNTLPTSAES 135

Query: 124 SKLTSSTWVELYIES 139
           S   SSTW+ELY ES
Sbjct: 136 SISNSSTWLELYFES 140

[Close]
BLAST of Spg032482 vs. ExPASy TrEMBL
Match: A0A5D3D5E2 (Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00700 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-12
Identity = 50/86 (58.14%), Postives = 57/86 (66.28%), Query Frame = 0 

Query: 53  EFMSKKLTRSRPRENRLSLQARELAKTPEAIWYRVHTGYIPLLTLSVLRDNDAFVEIELP 112
           +F + K  R +PRENR SLQ  EL KT             PL TLSVLRDNDA VEIEL 
Sbjct: 366 DFQTDKDAR-KPRENRPSLQTGELDKTV----------IFPLSTLSVLRDNDAIVEIELL 425

Query: 113 VPNTLPTSAEGSKLTSSTWVELYIES 139
           VP+TLPTSAE S+  S+TW+ELY ES
Sbjct: 426 VPDTLPTSAESSESNSNTWLELYFES 440

[Close]
BLAST of Spg032482 vs. ExPASy TrEMBL
Match: A0A5D3CCC7 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold314G00430 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.3e-12
Identity = 54/95 (56.84%), Postives = 63/95 (66.32%), Query Frame = 0 

Query: 64   PRENRLSLQARELAKT------PEAIWYR----VHTGYI-PLLTLSVLRDNDAFVEIELP 123
            PRENRLSLQ  EL KT       E +  R    V T  I PL TLSVLRDN+A VEIELP
Sbjct: 1265 PRENRLSLQTGELDKTLSIDYMTEILSLRLDGTVCTQVIFPLSTLSVLRDNNAVVEIELP 1324

Query: 124  VPNTLPTSAEGSKLTSSTWVELYIESFCIVNVFVR 148
            VP+TLPTSAE  +  SSTW+ELY+ES  +  +F +
Sbjct: 1325 VPDTLPTSAEIFESNSSTWLELYLESVHVEMLFYK 1359

The following BLAST results are available for this feature:
BLAST of Spg032482 vs. NCBI nr
Match NameE-valueIdentityDescription
KAA0037514.11.1e-1352.78reverse transcriptase [Cucumis melo var. makuwa][more]
TYK01989.11.1e-1352.78reverse transcriptase [Cucumis melo var. makuwa][more]
TYK01618.12.1e-1265.33uncharacterized protein E5676_scaffold451G002250 [Cucumis melo var. makuwa][more]
KAA0057197.12.7e-1258.14gag/pol protein [Cucumis melo var. makuwa][more]
TYK08952.12.7e-1256.84DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa][more]
BLAST of Spg032482 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Spg032482 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3BV685.4e-1452.78Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold80... [more]
A0A5A7T2235.4e-1452.78Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27... [more]
A0A5D3BRI21.0e-1265.33Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A5D3D5E21.3e-1258.14Gag/pol protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G0070... [more]
A0A5D3CCC71.3e-1256.84DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
BLAST of Spg032482 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg032482.1Spg032482.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity