Spg032438 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg032438
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionULP_PROTEASE domain-containing protein
Locationscaffold2: 29374101 .. 29377180 (+)
RNA-Seq ExpressionSpg032438
SyntenySpg032438
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAGTGAAGATGATGTATCACTCCATAGTGATGATGATGTGCTGAGTGAAGACAATATTCCTGAGACATCATGTAAGCGAGCTACACGTGGTCCTACTACCATGATTGAGCTCACTCAAATAAGGAGAAAGACGGATCGCAAAGTAATCGAATACCATGCACTTGGACAGTGGATTGGTAAAAACGCAACAAAAATGGTAAGTTATATTGGAATGGCTGTGCGAAGCCACATCCCAATCACATATAGCTCTTGGCCTCAAGTGCCAAGAGAGTTGAAGAAGAAAATTTACGAGATGATTGAGGTAATGTGTGTTGATTCACTATGTAGTTAAATAAATCATGTTATTAGTTTGATTTACTACACTTACATATTAACTTGTGTTTGTTAGGCTTCTTTTGTTACAAATCCCAAATAAAAAAAAGACATACTGAAGAAAGCTGACATCGCTTTCCGTCAGTTCAAGAACATTTTAAGGACAAAATATATTGTACCATTTCAAGACAATCTAGACCATTTGGCATTCCCACCACCATCATATTCACATATCGAACAAGCTCAATGGGATGCTTTCGTGAGTAATGTCTTATCGATAGATTTTCAAGTAAGTATTAAAGTTTATATATTTTCTATAGTATGTTTGATTTGTCTTATCTAATTGATTTGATTTGTGTAACTCATATATGATGTAGGAATTTAGCAAAATGCAAAAGAATAGACGTTCAAAGCACAAGTATAACCATAGACTTGCCCGTAAAGGATATGCAAACATGGTAGAAGAGTTGGTATGTTTTATTTTTTTTCGAATTTTATGAAGTTATGAAAGTTCATATAAAATTATTGTTATTTCATGCTTATATTCTTATGTTTGAATAATTCAATTTTTGTAGAATAAGGAAGGAAAAATATCTGGAAATGTGGATCGATCTATTCTTTGGGTCCATGCTTGAAAAGGAAAAACTAGAACATTCTTAAGTAAAGATATTGAGGATGCTGCTGCAAAAATTGTAAGTAACGTATCTATTATTTACATATATTACAATGATATATTTTTCTTGAGAAATCATTTTTTTATCGTTTTCAAATTATAGGATGAACTATCAAAAGATGCAAATTGTCAAAATGTTAGTTCAGATGACATTTTGACACAAGTTTTGGATACTCGTGAACACTCAGGACGTGTAAGAGGCATTGGTGGATTAATTGTACCTTCCTACTTCTTTCACAAGTCAACTCCATCAATTCCTAAGAATGTCGTAGTAGAGGATGAACCAGAAGGTTGGAAAAAATAAAAGGCAGAACTCCAGAGACAATAAAATGAAATGGAGAAGAAATTCCGTGAAATGCAGTCTCAAATGGAAAGTGGTAGACTAACACCAATGTCAGATCGAGGTAGTTGCCCACAAGTACCAAACCCTCAGCCTTCTGATGAAAAACCTGACTAGGTAACAATATTGGAATTATATTGAAATGTATTTTATGACAATACTTGATATATATAATATTAAATGAAAGTTCTAATATATAATTTTAAGTTTAGGGGAGATCATATAAACCTGCTGTTGAAGATGTAAAGAATGTTGTGGAGGCAGGAATTGTATATAAGAGGAAAGGCGACCATGAAATTGTTTATGGTGTCCCGCTCACAGCAAACTATGTGGGAGTATTGATTGAAGTAGTACATGACGCTGATGCTCCTTTGCCCATACCTATAGTTGGAAGTATTGAGTCTGTATTCGACGCAGTAGGCTCTCATGTTCCATGGCCTAAAGAACTCGTAATTCTGGACAAAGAAAAAAAGGTATGTTTAGTTAAGAAGCACATGATATATTAGTTTATATTATATATATATATTTTTTAATAAGGAATACTTTTTACGTAGGAACCTAAGAAAATGCATCGTGAATTAGCTGCAATAAAGGAGAAGAAGTCAAATAGTCAAGTGTCAAAGGTGCCGGTAGCAGTGAAATTCGTATTAGGATTTATAGAGAACATGACAAGGGATTACTTAGATATTACAGTACCCGATCATGTGTTCGGCTACCACTTCGACTTCCATCTAATGAAAAATAGCATGAAAGAATTTTGCTTGATGGAGGAATTAGCAACCACTGTTATAACAATTTATATCGCGTAAGTAACATTTACTTTAACAAATTGTCATTAATTTTCTTATGTTTTTAATACTAATATGAATTTATTTTGCTTTTAGATACCTATGCGAGTCTGACCCATCCATCTTAGAGCAATATGCATTTCTACATCCAGAATTGATCTCAGTTGGCTCGAGACCTGAAGAGAACCGCTCTCGAGCCCTATGTAAAAGGTTAAGATAGGTTAATGACAAATTATTGATATGTCCTTTTAATCCCGCGTAAGTATGAAAATCAAATATATGTTAACACTTTGCACTTTTTTTTTTTTGCTATAACTCTATTGTATGATTGTTGTCTATATAGATATCATTGGATGTTGTTGGTGATATCATTAAAAACATTCACAATTTATTCAATTGACTCTCTGAAGCATGACTTTTGTGATGATGTAAAGAACATGGTTAATACGTAAGTAAATAGTTATTTTAGCATATGTAAGTTTATTATTTATATTTTTTTAGTATTCTTATTATTATATTATTGTATCAGGGCTATAAGAATGTTCTATTCTCAAACTAATATACGAAGTCCCCTCTTCAAATGGGTATATGTTAAGGTAATGACTTTTACTTATTTTTTAGGTTGTTTTAATCATATTATACAAATTCTCTTATTACATGTGTTGAACTATATATGTAGTGTGCTCAACAATTAGGATCTACGGAGTGTGGATACTACACTCTTAAATTTATACGAGATATAGTATCCCATAGGAGCAGAGTGATTACAGATGTGGTAAATTAAGATCGATTTAGCCTTGTATTCAACTTATCTTTTTATATTTATTATAATTTTTGTAATGTAGTATGTTTACTTAATTTTTTTTTGTTTGCAGTTGACGAGAGGAACTGCATTTAGCCAATCAGAATTCGATGAGATAAGAGTTGAATTATGTGAATATGTAGCACAATACATGAGATGA

mRNA sequence

ATGGCTAGTGAAGATGATGTATCACTCCATAGTGATGATGATGTGCTGAGTGAAGACAATATTCCTGAGACATCATGTAAGCGAGCTACACGTGGTCCTACTACCATGATTGAGCTCACTCAAATAAGGAGAAAGACGGATCGCAAAGTAATCGAATACCATGCACTTGGACAGTGGATTGGTAAAAACGCAACAAAAATGGTAAGTTATATTGGAATGGCTGTGCGAAGCCACATCCCAATCACATATAGCTCTTGGCCTCAAGTGCCAAGAGAGTTGAAGAAGAAAATTTACGAGATGATTGAGTTCAAGAACATTTTAAGGACAAAATATATTGTACCATTTCAAGACAATCTAGACCATTTGGCATTCCCACCACCATCATATTCACATATCGAACAAGCTCAATGGGATGCTTTCGTGAGTAATGTCTTATCGATAGATTTTCAAGAATTTAGCAAAATGCAAAAGAATAGACGTTCAAAGCACAAGTATAACCATAGACTTGCCCGTAAAGGATATGCAAACATGGTAGAAGAGTTGGATGAACTATCAAAAGATGCAAATTGTCAAAATGTTAGTTCAGATGACATTTTGACACAAGTTTTGGATACTCGTGAACACTCAGGACGTGTAAGAGGCATTGGTGGATTAATTGTACCTTCCTACTTCTTTCACAAGTCAACTCCATCAATTCCTAAGAATGTCGTAGTAGAGGATGAACCAGAAGGAATTGTATATAAGAGGAAAGGCGACCATGAAATTGTTTATGGTGTCCCGCTCACAGCAAACTATGTGGGAGTATTGATTGAAGTAGTACATGACGCTGATGCTCCTTTGCCCATACCTATAGTTGGAAGTATTGAGTCTGTATTCGACGCAGTAGGCTCTCATGTTCCATGGCCTAAAGAACTCGTAATTCTGGACAAAGAAAAAAAGGAACCTAAGAAAATGCATCGTGAATTAGCTGCAATAAAGGAGAAGAAGTCAAATAGTCAAGTGTCAAAGGTGCCGGTAGCAGTGAAATTCGTATTAGGATTTATAGAGAACATGACAAGGGATTACTTAGATATTACAGTACCCGATCATGTGTTCGGCTACCACTTCGACTTCCATCTAATGAAAAATAGCATGAAAGAATTTTGCTTGATGGAGGAATTAGCAACCACTGTTATAACAATTTATATCGCATACCTATGCGAGTCTGACCCATCCATCTTAGAGCAATATGCATTTCTACATCCAGAATTGATCTCAGTTGGCTCGAGACCTGAAGAGAACCGCTCTCGAGCCCTATGTAAAAGATATCATTGGATGTTGTTGGTGATATCATTAAAAACATTCACAATTTATTCAATTGACTCTCTGAAGCATGACTTTTGTGATGATGTAAAGAACATGGTTAATACGGCTATAAGAATGTTCTATTCTCAAACTAATATACGAAGTCCCCTCTTCAAATGGGTATATGTTAAGTGTGCTCAACAATTAGGATCTACGGAGTGTGGATACTACACTCTTAAATTTATACGAGATATAGTATCCCATAGGAGCAGAGTGATTACAGATGTGTTGACGAGAGGAACTGCATTTAGCCAATCAGAATTCGATGAGATAAGAGTTGAATTATGTGAATATGTAGCACAATACATGAGATGA

Coding sequence (CDS)

ATGGCTAGTGAAGATGATGTATCACTCCATAGTGATGATGATGTGCTGAGTGAAGACAATATTCCTGAGACATCATGTAAGCGAGCTACACGTGGTCCTACTACCATGATTGAGCTCACTCAAATAAGGAGAAAGACGGATCGCAAAGTAATCGAATACCATGCACTTGGACAGTGGATTGGTAAAAACGCAACAAAAATGGTAAGTTATATTGGAATGGCTGTGCGAAGCCACATCCCAATCACATATAGCTCTTGGCCTCAAGTGCCAAGAGAGTTGAAGAAGAAAATTTACGAGATGATTGAGTTCAAGAACATTTTAAGGACAAAATATATTGTACCATTTCAAGACAATCTAGACCATTTGGCATTCCCACCACCATCATATTCACATATCGAACAAGCTCAATGGGATGCTTTCGTGAGTAATGTCTTATCGATAGATTTTCAAGAATTTAGCAAAATGCAAAAGAATAGACGTTCAAAGCACAAGTATAACCATAGACTTGCCCGTAAAGGATATGCAAACATGGTAGAAGAGTTGGATGAACTATCAAAAGATGCAAATTGTCAAAATGTTAGTTCAGATGACATTTTGACACAAGTTTTGGATACTCGTGAACACTCAGGACGTGTAAGAGGCATTGGTGGATTAATTGTACCTTCCTACTTCTTTCACAAGTCAACTCCATCAATTCCTAAGAATGTCGTAGTAGAGGATGAACCAGAAGGAATTGTATATAAGAGGAAAGGCGACCATGAAATTGTTTATGGTGTCCCGCTCACAGCAAACTATGTGGGAGTATTGATTGAAGTAGTACATGACGCTGATGCTCCTTTGCCCATACCTATAGTTGGAAGTATTGAGTCTGTATTCGACGCAGTAGGCTCTCATGTTCCATGGCCTAAAGAACTCGTAATTCTGGACAAAGAAAAAAAGGAACCTAAGAAAATGCATCGTGAATTAGCTGCAATAAAGGAGAAGAAGTCAAATAGTCAAGTGTCAAAGGTGCCGGTAGCAGTGAAATTCGTATTAGGATTTATAGAGAACATGACAAGGGATTACTTAGATATTACAGTACCCGATCATGTGTTCGGCTACCACTTCGACTTCCATCTAATGAAAAATAGCATGAAAGAATTTTGCTTGATGGAGGAATTAGCAACCACTGTTATAACAATTTATATCGCATACCTATGCGAGTCTGACCCATCCATCTTAGAGCAATATGCATTTCTACATCCAGAATTGATCTCAGTTGGCTCGAGACCTGAAGAGAACCGCTCTCGAGCCCTATGTAAAAGATATCATTGGATGTTGTTGGTGATATCATTAAAAACATTCACAATTTATTCAATTGACTCTCTGAAGCATGACTTTTGTGATGATGTAAAGAACATGGTTAATACGGCTATAAGAATGTTCTATTCTCAAACTAATATACGAAGTCCCCTCTTCAAATGGGTATATGTTAAGTGTGCTCAACAATTAGGATCTACGGAGTGTGGATACTACACTCTTAAATTTATACGAGATATAGTATCCCATAGGAGCAGAGTGATTACAGATGTGTTGACGAGAGGAACTGCATTTAGCCAATCAGAATTCGATGAGATAAGAGTTGAATTATGTGAATATGTAGCACAATACATGAGATGA

Protein sequence

MASEDDVSLHSDDDVLSEDNIPETSCKRATRGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATKMVSYIGMAVRSHIPITYSSWPQVPRELKKKIYEMIEFKNILRTKYIVPFQDNLDHLAFPPPSYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHKYNHRLARKGYANMVEELDELSKDANCQNVSSDDILTQVLDTREHSGRVRGIGGLIVPSYFFHKSTPSIPKNVVVEDEPEGIVYKRKGDHEIVYGVPLTANYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPWPKELVILDKEKKEPKKMHRELAAIKEKKSNSQVSKVPVAVKFVLGFIENMTRDYLDITVPDHVFGYHFDFHLMKNSMKEFCLMEELATTVITIYIAYLCESDPSILEQYAFLHPELISVGSRPEENRSRALCKRYHWMLLVISLKTFTIYSIDSLKHDFCDDVKNMVNTAIRMFYSQTNIRSPLFKWVYVKCAQQLGSTECGYYTLKFIRDIVSHRSRVITDVLTRGTAFSQSEFDEIRVELCEYVAQYMR
Homology
BLAST of Spg032438 vs. NCBI nr
Match: TYK07775.1 (putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa] >TYK08446.1 putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa])

HSP 1 Score: 323.6 bits (828), Expect = 3.4e-84
Identity = 211/580 (36.38%), Postives = 311/580 (53.62%), Query Frame = 0

Query: 31  RGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATKMVSYIGMAVRSHIPITYSSWPQVP 90
           RGPT M ++T+IR   D+KV+ Y+  G  IG+N  K+ S+IG A   H+PITY SW  VP
Sbjct: 28  RGPTIMFDVTRIRSLGDKKVVRYNEDGAPIGENGAKLKSFIGSATHYHVPITYMSWKSVP 87

Query: 91  RELKKKIYEMIE-----------------------FKNILRTKYIVPFQDNLDHLAFPPP 150
            ELK KI+  +E                       FKN L TKYI+P +D    L  PP 
Sbjct: 88  AELKDKIFTTVEAAFVIDPRSRKNVLQTAGISFRQFKNWLTTKYIIPHKDEPQLLQVPPE 147

Query: 151 SYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHKYNHRLARKGYANMVEELDELSKD 210
            YS IEQ  W+ FV + LS  FQE  K+Q++RRSK+KYNHR++RKGYAN+ EE+DE+S +
Sbjct: 148 KYSFIEQNHWEEFVRSRLSETFQEKRKLQQDRRSKNKYNHRISRKGYANLKEEMDEISMN 207

Query: 211 ANCQNVS---SDDILTQVLDTREHSGRVRGIGGLIVPSYFFH--KSTPSIPKNVVVEDEP 270
            +  +V+   S+D+LTQ L T+EH+GRVRG+GG + P+ +FH  K T     N++VE+E 
Sbjct: 208 TDSSSVNRHCSNDVLTQALGTKEHNGRVRGVGGYVTPTTYFHSVKKTSKDEANILVENEE 267

Query: 271 -EGIVYKRKGDHEIVYGVPLTANYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPW 330
               V + +         PL+A+                PI + G  E         +  
Sbjct: 268 LRRRVSELEAQIRSNLSTPLSAH-----------GSCSRPIVLEGIEEKGKRIEVESLDK 327

Query: 331 PKELVILDKEKKEPKKMHR-ELAAIKEK---KSNSQV-----SKVPVAVKFVLGFIENMT 390
           PK     +K+ KE  KM+  EL  +KE+   K  ++V     S +P+A+K +L + E + 
Sbjct: 328 PKH---KEKKGKEVMKMNEPELDVLKERDLIKMPTEVVCESTSTLPLALKSILRYAEKVM 387

Query: 391 RDYLDIT--VPDHVFGYHFDFHLMKNSMKEFCLMEELATTVITIYIAYLCESDPSILE-- 450
                IT  +P  +FG      +++  + + C M E+ T  +  Y+ YL  S     E  
Sbjct: 388 EKDSSITFSLPADLFGVSRKTSVLREDIVDLCNMNEVKTFTLVAYMMYLYSSVSGSKENM 447

Query: 451 QYAFLHPELISVGSRPEENRSRALCKRY-----------------HWMLLVISLKTFTIY 510
           QY F+ P LIS G+  +E+R R LC R                  HW LL I+    T++
Sbjct: 448 QYVFVDPSLISSGN-TQESRIRNLCSRLMVSKPDQVVLAPFNPGGHWALLAINAYEDTVF 507

Query: 511 SIDSLKHDFCDDVKNMVNTAIRMFYSQTNIRSPLFKWVYVKCAQQLGSTECGYYTLKFIR 552
            +DSL+       + + +TAI +F+SQ NI     + ++  C  Q+GST CGYY +K++R
Sbjct: 508 YLDSLRTTSKATTRYVTDTAIAIFHSQKNINKSRKQTLWRTCPLQVGSTTCGYYVMKYMR 567

BLAST of Spg032438 vs. NCBI nr
Match: TYK07544.1 (putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa] >TYK29038.1 putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa])

HSP 1 Score: 322.4 bits (825), Expect = 7.6e-84
Identity = 210/582 (36.08%), Postives = 311/582 (53.44%), Query Frame = 0

Query: 31  RGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATKMVSYIGMAVRSHIPITYSSWPQVP 90
           RGPT M ++T+IR   D+KV+ Y+  G  IG+N  K+ S+IG A   H+PITY SW  VP
Sbjct: 28  RGPTIMFDVTRIRSLGDKKVVRYNEDGAPIGENGAKLKSFIGSATHYHVPITYMSWKSVP 87

Query: 91  RELKKKIYEMIE-----------------------FKNILRTKYIVPFQDNLDHLAFPPP 150
            ELK KI+  +E                       FKN L TKYI+P +D    L  PP 
Sbjct: 88  AELKDKIFTTVEAAFVIDPRSRKNVLQTAGISFRQFKNWLTTKYIIPHKDEPQLLQVPPE 147

Query: 151 SYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHKYNHRLARKGYANMVEELDELSKD 210
            YS IEQ  W+ FV + LS  FQE  K+Q++RRSK+KYNHR++RKGYAN+ EE+DE+S +
Sbjct: 148 KYSFIEQNHWEEFVRSRLSETFQEKRKLQQDRRSKNKYNHRISRKGYANLKEEMDEISMN 207

Query: 211 ANCQNVS---SDDILTQVLDTREHSGRVRGIGGLIVPSYFFH--KSTPSIPKNVVVEDEP 270
            +  +V+   S+D+LTQ L T+EH+GRVRG+GG + P+ +FH  K T     N++VE+E 
Sbjct: 208 TDSSSVNRHCSNDVLTQALGTKEHNGRVRGVGGYVTPTTYFHSVKKTSKDEANILVENEE 267

Query: 271 -EGIVYKRKGDHEIVYGVPLTANYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPW 330
               V + +         PL+A+                PI + G  E         +  
Sbjct: 268 LRRRVSELEAQIRSNLSTPLSAH-----------GSCSRPIVLEGIEEKGKRIEVESLDK 327

Query: 331 PKELVILDKEKKEPKKMHR-ELAAIKEK-----KSNSQV-----SKVPVAVKFVLGFIEN 390
           PK+    +K+ KE  KM+  EL  +KE+      +  +V     S +P+A+K +L + E 
Sbjct: 328 PKQ---KEKKGKEVMKMNEPELDVLKERDLIKMPTEKEVVCESTSTLPLALKSILRYAEK 387

Query: 391 MTRDYLDIT--VPDHVFGYHFDFHLMKNSMKEFCLMEELATTVITIYIAYLCESDPSILE 450
           +      IT  +P  +FG      +++  + + C M E+ T  +  Y+ YL  S     E
Sbjct: 388 VMEKDSSITFSLPADLFGVSRKTSVLREDIVDLCNMNEVKTFTLVAYMMYLYSSVSGSKE 447

Query: 451 --QYAFLHPELISVGSRPEENRSRALCKRY-----------------HWMLLVISLKTFT 510
             QY F+ P LIS G+  +E+R R LC R                  HW LL I+    T
Sbjct: 448 NMQYVFVDPSLISSGN-TQESRIRNLCSRLMVSKPDQVVLAPFNPGGHWALLAINAYEDT 507

Query: 511 IYSIDSLKHDFCDDVKNMVNTAIRMFYSQTNIRSPLFKWVYVKCAQQLGSTECGYYTLKF 552
           ++ +DSL+       + + +TAI +F+SQ NI     + ++  C  Q+GST CGYY +K+
Sbjct: 508 VFYLDSLRTTSKATTRYVTDTAIAIFHSQKNINKSRKQTLWRTCPLQVGSTTCGYYVMKY 567

BLAST of Spg032438 vs. NCBI nr
Match: TYK24336.1 (putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa] >TYK24781.1 putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa])

HSP 1 Score: 321.6 bits (823), Expect = 1.3e-83
Identity = 210/580 (36.21%), Postives = 310/580 (53.45%), Query Frame = 0

Query: 31  RGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATKMVSYIGMAVRSHIPITYSSWPQVP 90
           RGPT M ++T+IR   D+KV+ Y+  G  IG+N  K+ S+IG A   H+PITY SW  VP
Sbjct: 28  RGPTIMFDVTRIRSLGDKKVVRYNEDGAPIGENGAKLKSFIGSATHYHVPITYMSWKSVP 87

Query: 91  RELKKKIYEMIE-----------------------FKNILRTKYIVPFQDNLDHLAFPPP 150
            ELK KI+  +E                       FKN L TKYI+P +D    L  PP 
Sbjct: 88  AELKDKIFTTVEAAFVIDPRSRKNVLQTAGISFRQFKNWLTTKYIIPHKDEPQLLQVPPE 147

Query: 151 SYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHKYNHRLARKGYANMVEELDELSKD 210
            YS IEQ  W+ FV + LS  FQE  K+Q++RRSK+KYNHR++RKGYAN+ EE+DE+S +
Sbjct: 148 KYSFIEQNHWEEFVRSRLSETFQEKRKLQQDRRSKNKYNHRISRKGYANLKEEMDEISMN 207

Query: 211 ANCQNVSS---DDILTQVLDTREHSGRVRGIGGLIVPSYFFH--KSTPSIPKNVVVEDEP 270
            +  +V+    +D+LTQ L T+EH+GRVRG+GG + P+ +FH  K T     N++VE+E 
Sbjct: 208 TDSSSVNRHCLNDVLTQALGTKEHNGRVRGVGGYVTPTTYFHSVKKTSKDEANILVENEE 267

Query: 271 -EGIVYKRKGDHEIVYGVPLTANYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPW 330
               V + +         PL+A+                PI + G  E         +  
Sbjct: 268 LRRRVSELEAQIRSNLSTPLSAH-----------GSCSRPIVLEGIEEKGKRIEVESLDK 327

Query: 331 PKELVILDKEKKEPKKMHR-ELAAIKEK---KSNSQV-----SKVPVAVKFVLGFIENMT 390
           PK     +K+ KE  KM+  EL  +KE+   K  ++V     S +P+A+K +L + E + 
Sbjct: 328 PKH---KEKKGKEVMKMNEPELDVLKERDLIKMPTEVVCESTSTLPLALKSILRYAEKVM 387

Query: 391 RDYLDIT--VPDHVFGYHFDFHLMKNSMKEFCLMEELATTVITIYIAYLCESDPSILE-- 450
                IT  +P  +FG      +++  + + C M E+ T  +  Y+ YL  S     E  
Sbjct: 388 EKDSSITFSLPADLFGVSRKTSVLREDIVDLCNMNEVKTFTLVAYMMYLYSSVSGSKENM 447

Query: 451 QYAFLHPELISVGSRPEENRSRALCKRY-----------------HWMLLVISLKTFTIY 510
           QY F+ P LIS G+  +E+R R LC R                  HW LL I+    T++
Sbjct: 448 QYVFVDPSLISSGN-TQESRIRNLCSRLMVSKPDQVVLAPFNPGGHWALLAINAYEDTVF 507

Query: 511 SIDSLKHDFCDDVKNMVNTAIRMFYSQTNIRSPLFKWVYVKCAQQLGSTECGYYTLKFIR 552
            +DSL+       + + +TAI +F+SQ NI     + ++  C  Q+GST CGYY +K++R
Sbjct: 508 YLDSLRTTSKATTRYVTDTAIAIFHSQKNINKSRKQTLWRTCPLQVGSTTCGYYVMKYMR 567

BLAST of Spg032438 vs. NCBI nr
Match: TYK22159.1 (putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa])

HSP 1 Score: 317.8 bits (813), Expect = 1.9e-82
Identity = 209/580 (36.03%), Postives = 309/580 (53.28%), Query Frame = 0

Query: 31  RGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATKMVSYIGMAVRSHIPITYSSWPQVP 90
           RGPT M  +T+ R   D+KV+ Y+     IG+N +K+ S+IG A   H+PITY SW  VP
Sbjct: 28  RGPTIMFHVTRTRSLGDKKVVRYNKDRAPIGENESKLKSFIGSATHYHVPITYMSWKSVP 87

Query: 91  RELKKKIYEMIE-----------------------FKNILRTKYIVPFQDNLDHLAFPPP 150
            ELK KI+  +E                       FKN L TKYI+P +D    L  PP 
Sbjct: 88  AELKDKIFTTVEAAFVIDPRSRKNVLQTAGISFRQFKNWLTTKYIIPHKDEPQLLQVPPE 147

Query: 151 SYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHKYNHRLARKGYANMVEELDELSKD 210
            YS IEQ  W+ FV + LS  FQE  K+Q++RRSK+KYNHR++RKGYAN+ EE+DE+S +
Sbjct: 148 KYSFIEQNHWEEFVRSRLSETFQEKRKLQQDRRSKNKYNHRISRKGYANLKEEMDEISMN 207

Query: 211 ANCQNVS---SDDILTQVLDTREHSGRVRGIGGLIVPSYFFH--KSTPSIPKNVVVEDEP 270
            +  +V+   S+D+LTQ L T+EH+GRVRG+GG + P+ +FH  K T     N++VE+E 
Sbjct: 208 TDSSSVNRHCSNDVLTQALGTKEHNGRVRGVGGYVTPTTYFHSVKKTSKDEANILVENEE 267

Query: 271 -EGIVYKRKGDHEIVYGVPLTANYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPW 330
               V + +         PL+A+                PI + G  E         +  
Sbjct: 268 LRRRVSELEAQIRSNLSTPLSAH-----------GSCSRPIVLEGIEEKGKRIEVESLDK 327

Query: 331 PKELVILDKEKKEPKKMHR-ELAAIKEK---KSNSQV-----SKVPVAVKFVLGFIENMT 390
           PK     +K+ KE  KM+  EL  +KE+   K  ++V     S +P+A+K +L + E + 
Sbjct: 328 PKH---KEKKGKEVMKMNEPELDVLKERDLIKMPTEVVCESTSTLPLALKSILRYAEKVM 387

Query: 391 RDYLDIT--VPDHVFGYHFDFHLMKNSMKEFCLMEELATTVITIYIAYLCESDPSILE-- 450
                IT  +P  +FG      +++  + + C M E+ T  +  Y+ YL  S     E  
Sbjct: 388 EKDSSITFSLPADLFGVSRKTSVLREDIVDLCNMNEVKTFTLVAYMMYLYSSVSGSKENM 447

Query: 451 QYAFLHPELISVGSRPEENRSRALCKRY-----------------HWMLLVISLKTFTIY 510
           QY F+ P LIS G+  +E+R R LC R                  HW LL I+    T++
Sbjct: 448 QYVFVDPSLISSGN-TQESRIRNLCSRLMVSKPDQVVLAPFNPGGHWALLAINAYEDTVF 507

Query: 511 SIDSLKHDFCDDVKNMVNTAIRMFYSQTNIRSPLFKWVYVKCAQQLGSTECGYYTLKFIR 552
            +DSL+       + + +TAI +F+SQ NI     + ++  C  Q+GST CGYY +K++R
Sbjct: 508 YLDSLRTTSKATTRYVTDTAIAIFHSQKNINKSRKQTLWRTCPLQVGSTTCGYYVMKYMR 567

BLAST of Spg032438 vs. NCBI nr
Match: XP_022136077.1 (uncharacterized protein LOC111007859 isoform X2 [Momordica charantia])

HSP 1 Score: 314.7 bits (805), Expect = 1.6e-81
Identity = 208/669 (31.09%), Postives = 332/669 (49.63%), Query Frame = 0

Query: 11  SDDDVLSEDN---IPETSCKR-ATRGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATK 70
           SD    S+D    +  T  K+ A RGPTTM ELT IR    RK IEY+  GQ IG+NA K
Sbjct: 2   SDSSSSSQDERSVVIHTEVKKVARRGPTTMHELTCIRNLGKRKTIEYNDQGQPIGENAKK 61

Query: 71  MVSYIGMAVRSHIPITYSSWPQVPRELKKKIYEMIE----------------------FK 130
           M S+IG+ VR  IP+TY+ W +VP+ELK KI+  +E                      FK
Sbjct: 62  MQSFIGVCVRQKIPVTYNHWKEVPQELKDKIFNCVESFVLDWRSKHHILQSASKKFRTFK 121

Query: 131 NILRTKYIVPFQDNLDHLAFPPPSYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHK 190
           + L   YI+PF+D    L  PP  Y HI+Q QW++FV+  LS +++  S+  K  R+K  
Sbjct: 122 STLTRTYILPFKDEPICLQNPPEKYPHIDQEQWNSFVNARLSEEWETLSRAHKEIRAKCL 181

Query: 191 YNHRLARKGYANMVEELDELSKDANCQNV------------------------------- 250
           YNH ++RKGYAN+ +ELD LS D + + +                               
Sbjct: 182 YNHHISRKGYANLAQELD-LSSDPSNRAILWKEARKGKNNEYFDDATRECAARIDELAAI 241

Query: 251 -SSDDILTQVLDTREHSGRVRGIGGLIVPSYFFH-------------------------- 310
              +DILT+ L T EHSGRVRG+G  + PS +F+                          
Sbjct: 242 HKGEDILTEALGTSEHSGRVRGVGEFVSPSLYFNVVKGKSKTQELQPNKSTTEGSNPSKK 301

Query: 311 ----KSTPSIPKNVVVEDEPE-------------------GIVYKRKGDHEIVYGVPLTA 370
               K   ++ + + V DE +                   G ++        V+GVPL  
Sbjct: 302 KSKGKEIVNVHEEIYVTDEQKMEGKPCHLAVESVDNIVAVGTIFDNNVQCPTVHGVPLGV 361

Query: 371 NYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPWPKELVILDKEKKEPKKMHRELA 430
           + V V++++V D  A +PIP+ G IE++   +G  V WP+ LVIL +EK         ++
Sbjct: 362 DNVRVMVDIVIDEYATIPIPVRGEIETLNQTIGGFVAWPRRLVILSEEK--------NIS 421

Query: 431 AIKEKKSNSQVSK---VPVAVKFVLGFI--ENMTRDYLDITVPDHVFGYHFDFHLMKNSM 490
           + +  ++ +Q+SK   V V++K +  ++       D ++I +   +FG   + +L +N +
Sbjct: 422 SSRTSQTRTQLSKHTDVHVSIKLLNRYVMLSMQHEDTVEINLSKDIFGKEKNIYLTRNDI 481

Query: 491 KEFCLMEELATTVITIYIAYLCE-SDPSILEQYAFLHPELISVGSRPEENRSRALCKRY- 548
            ++C M E+  + I  YIAYL +  +  I +++  + P  IS   + +E R R L  R  
Sbjct: 482 MQYCTMIEIGYSCILTYIAYLWDVYEYEITKKFLIVDPATISPYVKSQEYRFRNLANRLE 541

BLAST of Spg032438 vs. ExPASy TrEMBL
Match: A0A5D3C8G8 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1737G00550 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 1.7e-84
Identity = 211/580 (36.38%), Postives = 311/580 (53.62%), Query Frame = 0

Query: 31  RGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATKMVSYIGMAVRSHIPITYSSWPQVP 90
           RGPT M ++T+IR   D+KV+ Y+  G  IG+N  K+ S+IG A   H+PITY SW  VP
Sbjct: 28  RGPTIMFDVTRIRSLGDKKVVRYNEDGAPIGENGAKLKSFIGSATHYHVPITYMSWKSVP 87

Query: 91  RELKKKIYEMIE-----------------------FKNILRTKYIVPFQDNLDHLAFPPP 150
            ELK KI+  +E                       FKN L TKYI+P +D    L  PP 
Sbjct: 88  AELKDKIFTTVEAAFVIDPRSRKNVLQTAGISFRQFKNWLTTKYIIPHKDEPQLLQVPPE 147

Query: 151 SYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHKYNHRLARKGYANMVEELDELSKD 210
            YS IEQ  W+ FV + LS  FQE  K+Q++RRSK+KYNHR++RKGYAN+ EE+DE+S +
Sbjct: 148 KYSFIEQNHWEEFVRSRLSETFQEKRKLQQDRRSKNKYNHRISRKGYANLKEEMDEISMN 207

Query: 211 ANCQNVS---SDDILTQVLDTREHSGRVRGIGGLIVPSYFFH--KSTPSIPKNVVVEDEP 270
            +  +V+   S+D+LTQ L T+EH+GRVRG+GG + P+ +FH  K T     N++VE+E 
Sbjct: 208 TDSSSVNRHCSNDVLTQALGTKEHNGRVRGVGGYVTPTTYFHSVKKTSKDEANILVENEE 267

Query: 271 -EGIVYKRKGDHEIVYGVPLTANYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPW 330
               V + +         PL+A+                PI + G  E         +  
Sbjct: 268 LRRRVSELEAQIRSNLSTPLSAH-----------GSCSRPIVLEGIEEKGKRIEVESLDK 327

Query: 331 PKELVILDKEKKEPKKMHR-ELAAIKEK---KSNSQV-----SKVPVAVKFVLGFIENMT 390
           PK     +K+ KE  KM+  EL  +KE+   K  ++V     S +P+A+K +L + E + 
Sbjct: 328 PKH---KEKKGKEVMKMNEPELDVLKERDLIKMPTEVVCESTSTLPLALKSILRYAEKVM 387

Query: 391 RDYLDIT--VPDHVFGYHFDFHLMKNSMKEFCLMEELATTVITIYIAYLCESDPSILE-- 450
                IT  +P  +FG      +++  + + C M E+ T  +  Y+ YL  S     E  
Sbjct: 388 EKDSSITFSLPADLFGVSRKTSVLREDIVDLCNMNEVKTFTLVAYMMYLYSSVSGSKENM 447

Query: 451 QYAFLHPELISVGSRPEENRSRALCKRY-----------------HWMLLVISLKTFTIY 510
           QY F+ P LIS G+  +E+R R LC R                  HW LL I+    T++
Sbjct: 448 QYVFVDPSLISSGN-TQESRIRNLCSRLMVSKPDQVVLAPFNPGGHWALLAINAYEDTVF 507

Query: 511 SIDSLKHDFCDDVKNMVNTAIRMFYSQTNIRSPLFKWVYVKCAQQLGSTECGYYTLKFIR 552
            +DSL+       + + +TAI +F+SQ NI     + ++  C  Q+GST CGYY +K++R
Sbjct: 508 YLDSLRTTSKATTRYVTDTAIAIFHSQKNINKSRKQTLWRTCPLQVGSTTCGYYVMKYMR 567

BLAST of Spg032438 vs. ExPASy TrEMBL
Match: A0A5D3C8G6 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G001550 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 3.7e-84
Identity = 210/582 (36.08%), Postives = 311/582 (53.44%), Query Frame = 0

Query: 31  RGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATKMVSYIGMAVRSHIPITYSSWPQVP 90
           RGPT M ++T+IR   D+KV+ Y+  G  IG+N  K+ S+IG A   H+PITY SW  VP
Sbjct: 28  RGPTIMFDVTRIRSLGDKKVVRYNEDGAPIGENGAKLKSFIGSATHYHVPITYMSWKSVP 87

Query: 91  RELKKKIYEMIE-----------------------FKNILRTKYIVPFQDNLDHLAFPPP 150
            ELK KI+  +E                       FKN L TKYI+P +D    L  PP 
Sbjct: 88  AELKDKIFTTVEAAFVIDPRSRKNVLQTAGISFRQFKNWLTTKYIIPHKDEPQLLQVPPE 147

Query: 151 SYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHKYNHRLARKGYANMVEELDELSKD 210
            YS IEQ  W+ FV + LS  FQE  K+Q++RRSK+KYNHR++RKGYAN+ EE+DE+S +
Sbjct: 148 KYSFIEQNHWEEFVRSRLSETFQEKRKLQQDRRSKNKYNHRISRKGYANLKEEMDEISMN 207

Query: 211 ANCQNVS---SDDILTQVLDTREHSGRVRGIGGLIVPSYFFH--KSTPSIPKNVVVEDEP 270
            +  +V+   S+D+LTQ L T+EH+GRVRG+GG + P+ +FH  K T     N++VE+E 
Sbjct: 208 TDSSSVNRHCSNDVLTQALGTKEHNGRVRGVGGYVTPTTYFHSVKKTSKDEANILVENEE 267

Query: 271 -EGIVYKRKGDHEIVYGVPLTANYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPW 330
               V + +         PL+A+                PI + G  E         +  
Sbjct: 268 LRRRVSELEAQIRSNLSTPLSAH-----------GSCSRPIVLEGIEEKGKRIEVESLDK 327

Query: 331 PKELVILDKEKKEPKKMHR-ELAAIKEK-----KSNSQV-----SKVPVAVKFVLGFIEN 390
           PK+    +K+ KE  KM+  EL  +KE+      +  +V     S +P+A+K +L + E 
Sbjct: 328 PKQ---KEKKGKEVMKMNEPELDVLKERDLIKMPTEKEVVCESTSTLPLALKSILRYAEK 387

Query: 391 MTRDYLDIT--VPDHVFGYHFDFHLMKNSMKEFCLMEELATTVITIYIAYLCESDPSILE 450
           +      IT  +P  +FG      +++  + + C M E+ T  +  Y+ YL  S     E
Sbjct: 388 VMEKDSSITFSLPADLFGVSRKTSVLREDIVDLCNMNEVKTFTLVAYMMYLYSSVSGSKE 447

Query: 451 --QYAFLHPELISVGSRPEENRSRALCKRY-----------------HWMLLVISLKTFT 510
             QY F+ P LIS G+  +E+R R LC R                  HW LL I+    T
Sbjct: 448 NMQYVFVDPSLISSGN-TQESRIRNLCSRLMVSKPDQVVLAPFNPGGHWALLAINAYEDT 507

Query: 511 IYSIDSLKHDFCDDVKNMVNTAIRMFYSQTNIRSPLFKWVYVKCAQQLGSTECGYYTLKF 552
           ++ +DSL+       + + +TAI +F+SQ NI     + ++  C  Q+GST CGYY +K+
Sbjct: 508 VFYLDSLRTTSKATTRYVTDTAIAIFHSQKNINKSRKQTLWRTCPLQVGSTTCGYYVMKY 567

BLAST of Spg032438 vs. ExPASy TrEMBL
Match: A0A5D3DME5 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold184G00220 PE=3 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 6.3e-84
Identity = 210/580 (36.21%), Postives = 310/580 (53.45%), Query Frame = 0

Query: 31  RGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATKMVSYIGMAVRSHIPITYSSWPQVP 90
           RGPT M ++T+IR   D+KV+ Y+  G  IG+N  K+ S+IG A   H+PITY SW  VP
Sbjct: 28  RGPTIMFDVTRIRSLGDKKVVRYNEDGAPIGENGAKLKSFIGSATHYHVPITYMSWKSVP 87

Query: 91  RELKKKIYEMIE-----------------------FKNILRTKYIVPFQDNLDHLAFPPP 150
            ELK KI+  +E                       FKN L TKYI+P +D    L  PP 
Sbjct: 88  AELKDKIFTTVEAAFVIDPRSRKNVLQTAGISFRQFKNWLTTKYIIPHKDEPQLLQVPPE 147

Query: 151 SYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHKYNHRLARKGYANMVEELDELSKD 210
            YS IEQ  W+ FV + LS  FQE  K+Q++RRSK+KYNHR++RKGYAN+ EE+DE+S +
Sbjct: 148 KYSFIEQNHWEEFVRSRLSETFQEKRKLQQDRRSKNKYNHRISRKGYANLKEEMDEISMN 207

Query: 211 ANCQNVSS---DDILTQVLDTREHSGRVRGIGGLIVPSYFFH--KSTPSIPKNVVVEDEP 270
            +  +V+    +D+LTQ L T+EH+GRVRG+GG + P+ +FH  K T     N++VE+E 
Sbjct: 208 TDSSSVNRHCLNDVLTQALGTKEHNGRVRGVGGYVTPTTYFHSVKKTSKDEANILVENEE 267

Query: 271 -EGIVYKRKGDHEIVYGVPLTANYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPW 330
               V + +         PL+A+                PI + G  E         +  
Sbjct: 268 LRRRVSELEAQIRSNLSTPLSAH-----------GSCSRPIVLEGIEEKGKRIEVESLDK 327

Query: 331 PKELVILDKEKKEPKKMHR-ELAAIKEK---KSNSQV-----SKVPVAVKFVLGFIENMT 390
           PK     +K+ KE  KM+  EL  +KE+   K  ++V     S +P+A+K +L + E + 
Sbjct: 328 PKH---KEKKGKEVMKMNEPELDVLKERDLIKMPTEVVCESTSTLPLALKSILRYAEKVM 387

Query: 391 RDYLDIT--VPDHVFGYHFDFHLMKNSMKEFCLMEELATTVITIYIAYLCESDPSILE-- 450
                IT  +P  +FG      +++  + + C M E+ T  +  Y+ YL  S     E  
Sbjct: 388 EKDSSITFSLPADLFGVSRKTSVLREDIVDLCNMNEVKTFTLVAYMMYLYSSVSGSKENM 447

Query: 451 QYAFLHPELISVGSRPEENRSRALCKRY-----------------HWMLLVISLKTFTIY 510
           QY F+ P LIS G+  +E+R R LC R                  HW LL I+    T++
Sbjct: 448 QYVFVDPSLISSGN-TQESRIRNLCSRLMVSKPDQVVLAPFNPGGHWALLAINAYEDTVF 507

Query: 511 SIDSLKHDFCDDVKNMVNTAIRMFYSQTNIRSPLFKWVYVKCAQQLGSTECGYYTLKFIR 552
            +DSL+       + + +TAI +F+SQ NI     + ++  C  Q+GST CGYY +K++R
Sbjct: 508 YLDSLRTTSKATTRYVTDTAIAIFHSQKNINKSRKQTLWRTCPLQVGSTTCGYYVMKYMR 567

BLAST of Spg032438 vs. ExPASy TrEMBL
Match: A0A5D3DFW5 (Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold333G00120 PE=3 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 9.1e-83
Identity = 209/580 (36.03%), Postives = 309/580 (53.28%), Query Frame = 0

Query: 31  RGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATKMVSYIGMAVRSHIPITYSSWPQVP 90
           RGPT M  +T+ R   D+KV+ Y+     IG+N +K+ S+IG A   H+PITY SW  VP
Sbjct: 28  RGPTIMFHVTRTRSLGDKKVVRYNKDRAPIGENESKLKSFIGSATHYHVPITYMSWKSVP 87

Query: 91  RELKKKIYEMIE-----------------------FKNILRTKYIVPFQDNLDHLAFPPP 150
            ELK KI+  +E                       FKN L TKYI+P +D    L  PP 
Sbjct: 88  AELKDKIFTTVEAAFVIDPRSRKNVLQTAGISFRQFKNWLTTKYIIPHKDEPQLLQVPPE 147

Query: 151 SYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHKYNHRLARKGYANMVEELDELSKD 210
            YS IEQ  W+ FV + LS  FQE  K+Q++RRSK+KYNHR++RKGYAN+ EE+DE+S +
Sbjct: 148 KYSFIEQNHWEEFVRSRLSETFQEKRKLQQDRRSKNKYNHRISRKGYANLKEEMDEISMN 207

Query: 211 ANCQNVS---SDDILTQVLDTREHSGRVRGIGGLIVPSYFFH--KSTPSIPKNVVVEDEP 270
            +  +V+   S+D+LTQ L T+EH+GRVRG+GG + P+ +FH  K T     N++VE+E 
Sbjct: 208 TDSSSVNRHCSNDVLTQALGTKEHNGRVRGVGGYVTPTTYFHSVKKTSKDEANILVENEE 267

Query: 271 -EGIVYKRKGDHEIVYGVPLTANYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPW 330
               V + +         PL+A+                PI + G  E         +  
Sbjct: 268 LRRRVSELEAQIRSNLSTPLSAH-----------GSCSRPIVLEGIEEKGKRIEVESLDK 327

Query: 331 PKELVILDKEKKEPKKMHR-ELAAIKEK---KSNSQV-----SKVPVAVKFVLGFIENMT 390
           PK     +K+ KE  KM+  EL  +KE+   K  ++V     S +P+A+K +L + E + 
Sbjct: 328 PKH---KEKKGKEVMKMNEPELDVLKERDLIKMPTEVVCESTSTLPLALKSILRYAEKVM 387

Query: 391 RDYLDIT--VPDHVFGYHFDFHLMKNSMKEFCLMEELATTVITIYIAYLCESDPSILE-- 450
                IT  +P  +FG      +++  + + C M E+ T  +  Y+ YL  S     E  
Sbjct: 388 EKDSSITFSLPADLFGVSRKTSVLREDIVDLCNMNEVKTFTLVAYMMYLYSSVSGSKENM 447

Query: 451 QYAFLHPELISVGSRPEENRSRALCKRY-----------------HWMLLVISLKTFTIY 510
           QY F+ P LIS G+  +E+R R LC R                  HW LL I+    T++
Sbjct: 448 QYVFVDPSLISSGN-TQESRIRNLCSRLMVSKPDQVVLAPFNPGGHWALLAINAYEDTVF 507

Query: 511 SIDSLKHDFCDDVKNMVNTAIRMFYSQTNIRSPLFKWVYVKCAQQLGSTECGYYTLKFIR 552
            +DSL+       + + +TAI +F+SQ NI     + ++  C  Q+GST CGYY +K++R
Sbjct: 508 YLDSLRTTSKATTRYVTDTAIAIFHSQKNINKSRKQTLWRTCPLQVGSTTCGYYVMKYMR 567

BLAST of Spg032438 vs. ExPASy TrEMBL
Match: A0A6J1C4J7 (uncharacterized protein LOC111007859 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007859 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 7.7e-82
Identity = 208/669 (31.09%), Postives = 332/669 (49.63%), Query Frame = 0

Query: 11  SDDDVLSEDN---IPETSCKR-ATRGPTTMIELTQIRRKTDRKVIEYHALGQWIGKNATK 70
           SD    S+D    +  T  K+ A RGPTTM ELT IR    RK IEY+  GQ IG+NA K
Sbjct: 2   SDSSSSSQDERSVVIHTEVKKVARRGPTTMHELTCIRNLGKRKTIEYNDQGQPIGENAKK 61

Query: 71  MVSYIGMAVRSHIPITYSSWPQVPRELKKKIYEMIE----------------------FK 130
           M S+IG+ VR  IP+TY+ W +VP+ELK KI+  +E                      FK
Sbjct: 62  MQSFIGVCVRQKIPVTYNHWKEVPQELKDKIFNCVESFVLDWRSKHHILQSASKKFRTFK 121

Query: 131 NILRTKYIVPFQDNLDHLAFPPPSYSHIEQAQWDAFVSNVLSIDFQEFSKMQKNRRSKHK 190
           + L   YI+PF+D    L  PP  Y HI+Q QW++FV+  LS +++  S+  K  R+K  
Sbjct: 122 STLTRTYILPFKDEPICLQNPPEKYPHIDQEQWNSFVNARLSEEWETLSRAHKEIRAKCL 181

Query: 191 YNHRLARKGYANMVEELDELSKDANCQNV------------------------------- 250
           YNH ++RKGYAN+ +ELD LS D + + +                               
Sbjct: 182 YNHHISRKGYANLAQELD-LSSDPSNRAILWKEARKGKNNEYFDDATRECAARIDELAAI 241

Query: 251 -SSDDILTQVLDTREHSGRVRGIGGLIVPSYFFH-------------------------- 310
              +DILT+ L T EHSGRVRG+G  + PS +F+                          
Sbjct: 242 HKGEDILTEALGTSEHSGRVRGVGEFVSPSLYFNVVKGKSKTQELQPNKSTTEGSNPSKK 301

Query: 311 ----KSTPSIPKNVVVEDEPE-------------------GIVYKRKGDHEIVYGVPLTA 370
               K   ++ + + V DE +                   G ++        V+GVPL  
Sbjct: 302 KSKGKEIVNVHEEIYVTDEQKMEGKPCHLAVESVDNIVAVGTIFDNNVQCPTVHGVPLGV 361

Query: 371 NYVGVLIEVVHDADAPLPIPIVGSIESVFDAVGSHVPWPKELVILDKEKKEPKKMHRELA 430
           + V V++++V D  A +PIP+ G IE++   +G  V WP+ LVIL +EK         ++
Sbjct: 362 DNVRVMVDIVIDEYATIPIPVRGEIETLNQTIGGFVAWPRRLVILSEEK--------NIS 421

Query: 431 AIKEKKSNSQVSK---VPVAVKFVLGFI--ENMTRDYLDITVPDHVFGYHFDFHLMKNSM 490
           + +  ++ +Q+SK   V V++K +  ++       D ++I +   +FG   + +L +N +
Sbjct: 422 SSRTSQTRTQLSKHTDVHVSIKLLNRYVMLSMQHEDTVEINLSKDIFGKEKNIYLTRNDI 481

Query: 491 KEFCLMEELATTVITIYIAYLCE-SDPSILEQYAFLHPELISVGSRPEENRSRALCKRY- 548
            ++C M E+  + I  YIAYL +  +  I +++  + P  IS   + +E R R L  R  
Sbjct: 482 MQYCTMIEIGYSCILTYIAYLWDVYEYEITKKFLIVDPATISPYVKSQEYRFRNLANRLE 541

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK07775.13.4e-8436.38putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa] >TYK084... [more]
TYK07544.17.6e-8436.08putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa] >TYK290... [more]
TYK24336.11.3e-8336.21putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa] >TYK247... [more]
TYK22159.11.9e-8236.03putative serine/threonine-protein kinase nek2 [Cucumis melo var. makuwa][more]
XP_022136077.11.6e-8131.09uncharacterized protein LOC111007859 isoform X2 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3C8G81.7e-8436.38Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3C8G63.7e-8436.08Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3DME56.3e-8436.21Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3DFW59.1e-8336.03Putative serine/threonine-protein kinase nek2 OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1C4J77.7e-8231.09uncharacterized protein LOC111007859 isoform X2 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.395.10Adenoviral Proteinase; Chain Acoord: 431..549
e-value: 7.8E-12
score: 47.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR33018:SF19MYB/SANT-LIKE DNA-BINDING DOMAIN PROTEINcoord: 24..226
NoneNo IPR availablePANTHERPTHR33018OS10G0338966 PROTEIN-RELATEDcoord: 24..226
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 435..524
e-value: 7.8E-9
score: 35.7
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 350..546

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg032438.1Spg032438.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity