Spg032334 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg032334
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionUlp1-like peptidase
Locationscaffold2: 33082289 .. 33085299 (+)
RNA-Seq ExpressionSpg032334
SyntenySpg032334
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAACTTATTCATTTACTTTCTTTTGCGGGAAGTGAATGAGCCTAGGATTGATGTTATTAGCTTTGAGATTCTGGGGGAGAAAGTTTCATTTGGTCGGAGGGAATTTGACCTCATCACTGGAATTAGGCATAGGACCCAACATATTAGGGGTAATGTATCTAGTACTAGACTGAGAAGACTGTACCTTGACGATAGCATCAGCATGAAAGGGTTTGAACTAGATAAATTATTTCCTACCATTAATTTTGAGAGCGACGAGGATGCTGTGAAGATGGCTATATTTTATTTCATTGAGTTGGCTATGATGGGGAGGGAGAGAAAGCAGCAGATGGACACTAGCCTGCTCGGCATTATTGATGATTGGCAAAGGTTTTGTAATGAGGACTGGAGTAAGCTAATTTTTGATAAGACCATAAAGGGACTCAAGAAGGCAGTAGGTGGGAAGGCAGTGTCCTATAAAGAGAGGACAGATGGCAAACAGGAAACGTACAGTCTGTATGGCTTCCCATACGCGTTTCAGGTACGTTGGTTTAGAGTGATTGATAGTGTATACTTTCTCGATATATATTGTATCTCTAACAATATACTTTCTTTACTTACACAGGTATGGACATACGAGACTGTATCTTCGTTGACCGGGCGTGTGGCTAATCGCTTGAATGACAATGCCATTCCACGCATATTAAGATGGTCATGTAGCCACTCACCTACACTTGCAGCGCTGAGTCGGGAGGTGTTTTCTTCAGATATGGTATGTCGAATATATTTTATATGCATTCATTCAATTTAACTTTAGTAGGTTTATTTTTATAATTTTCCTTTTTCGTGCAGGCTAGGGTCACAACTGAACTTGTGGCCTCAGAAGAGGAGATCCAATTTATGGATCGTGTGATGCAGCCACCTCGAGCCTCATCTCCACCTCCACCTCCACCTCCACCTCCACCTCCACCTCCACCCCCAGTAGCTTTGGAAGATATTCCAGATGAAGATACTGTTGTTGAGGATCTCGGGACTGAGAATCCAAATGAAGTGGTGGAAGGTGTTGGGACGTCTGTTACGAATGACAGAGTCTGCAAGAGGTGCAAAGTCCTCGAAGACGAGATCAAAGTGATTAAAGAAGATGTGAAGGAGATTAAGGAGGATGTGAAAGTCATTAAGTCCATTGAAAAAGACCTGAAGGCGATAAGGAAGTTCATGCGTCGACTTTCGAAGGTATGTATAGTTATTTATTAATCGCTTCTATCAGTAGTATGACGTTTAGTTACTGATACATTCGTTTGACAGGGTAAATTCGTCGACGCCAGCAAGTATATAGAACCAGATGACGGTACAGACGATGGTGGTGGTGGATCTCGACCACATTCGAAAGGTCAGGATGATGGTGGTGGTCCTCCATCCGGGTCACAAGCAAAAGCAAATGACAACACCCCAATGGCTGACCATGCGGATCCGATGGATACAACAGAACAACATGGTCGAGTCGAGGAAGTAAATGACCCGATAGAGGGTGTGGGGAAAGACGTACAGATGGAGGTAACTGAAATAAGAGAACATGTAAGTGCAGTTTAATAGGAAGTTGGTTAATTTATGTTATTAGTGATTATAGTTTTTTTTTTTTTTTTTGGTCCATTAATAGGAAGTAATTGAAATAGGCGAACATGTAGATGCCCCGATAGAGGGTGTGGGAAAGGATATGCCTGTTGTCGAAAGTCAAAATTCTCTGGTAAGCATTCATTAGGGTTTAATATATTTATGGTTAGGGTTGATAGTGGTATTTAGGGTTAGGGTTATTGACTTTATTATCACTTTCCTTATTGTACAGGGTGTCCAGTCCATTTCTGAACAGAACGAGCCGATAGAAAGACGGGGGACTCGTAAGAGGAAGACTGCATGGAAGTTGAGAAGTCCATGGAAAGACACACGGGAAGACCGTAAGAAACGCAAGGCTCTGAAGTATGATCCTCTTCCCCAGATCCCCCACGATCTGGATGCTCCATTCAAAAGATGACTTGACACTGAGGACCCAGAAGACACAGTTCGGAAAACTGCGTATGCTGTCCGAGACAAGACGTGGTTTCGTGATCTTATCACTCCATCGAAATGGATGACCGATGAGGTATGGTTTGATTACTGACAATAATGATTCTTAATTGAAATACTAACACTGTAACTACGTGTAGGTGGTCGACTCGATTTTCATGTTCGTCCAAAAGAAATTGGGAGAACGACCAGATTTATGCCGCAGGAAGTTCACCATTGGGGACCTATGTGTAACGGTACGTACCATTGGAACAATTTGTGAATATGTTTGTTTGATAGTGTTCTATAGGGTTCTTATGTTTGGTATCTTCTTTTCATCAGAATTTCTTCAGACGCGAAGATGGCCTATACAAGGAAATGAGTAGCGGCATACATCCCCGAGACTTGACGTACGATTGGAGTAGCGCAGGCAATATCTTGAAGTACGGAATGGGCGAGCTTGCAGACCATAACATACCATGGAGCACGGTCGATTTGGTGTACATGCCGTATAACATCGGCGGGCTGCATTGGGTCCTCGTATGCATTGACCTCGAGGTGGGGGAGGTCGTTGTATCGGATTCCATGGTGGTGTTGAATAAGGACGAGGTGGTTGAGAAGGAGTTAAGGGTCCTTTGCCAAGTCCTGCCAGCTCTGCTTTGGAAGATCGGGGTCATGGATGTGAGGAAGAATCTCTCCGTTCAAAGATGGCCTCTACGTCGGGAATTGTCAAGGCCGCAGCAGAAACGTAGTGGCGACTGCGGTATGTTTGTATGTAAATTTTTTGAATATGATGTAACTGGGTTGCCGTATGACACCCTAAGTCAGGATAATATGATTTATTTTCGGTGGCAATATGCTGTACAGATATGGGCGAACAGACCCTTCTTCTAGTGGATTATATTGATTCAATCGTTTTTGTAATGAAATATTAAGTTTTTAGGGTTTATCATGTTTCCATGCAAACCATGCAAATTCTCATGCAAA

mRNA sequence

ATGGACAACTTATTCATTTACTTTCTTTTGCGGGAAGTGAATGAGCCTAGGATTGATGTTATTAGCTTTGAGATTCTGGGGGAGAAAGTTTCATTTGGTCGGAGGGAATTTGACCTCATCACTGGAATTAGGCATAGGACCCAACATATTAGGGGTAATGTATCTAGTACTAGACTGAGAAGACTGTACCTTGACGATAGCATCAGCATGAAAGGGTTTGAACTAGATAAATTATTTCCTACCATTAATTTTGAGAGCGACGAGGATGCTGTGAAGATGGCTATATTTTATTTCATTGAGTTGGCTATGATGGGGAGGGAGAGAAAGCAGCAGATGGACACTAGCCTGCTCGGCATTATTGATGATTGGCAAAGGTTTTGTAATGAGGACTGGAGTAAGCTAATTTTTGATAAGACCATAAAGGGACTCAAGAAGGCAGTAGGTGGGAAGGCAGTGTCCTATAAAGAGAGGACAGATGGCAAACAGGAAACGTACAGTCTGTATGGCTTCCCATACGCGTTTCAGGTATGGACATACGAGACTGTATCTTCGTTGACCGGGCGTGTGGCTAATCGCTTGAATGACAATGCCATTCCACGCATATTAAGATGGTCATGTAGCCACTCACCTACACTTGCAGCGCTGAGTCGGGAGGTGTTTTCTTCAGATATGGCTAGGGTCACAACTGAACTTGTGGCCTCAGAAGAGGAGATCCAATTTATGGATCGTGTGATGCAGCCACCTCGAGCCTCATCTCCACCTCCACCTCCACCTCCACCTCCACCTCCACCTCCACCCCCAGTAGCTTTGGAAGATATTCCAGATGAAGATACTGTTGTTGAGGATCTCGGGACTGAGAATCCAAATGAAGTGGTGGAAGGTGTTGGGACGTCTGTTACGAATGACAGAGTCTGCAAGAGGTGCAAAGTCCTCGAAGACGAGATCAAAGTGATTAAAGAAGATGTGAAGGAGATTAAGGAGGATGTGAAAGTCATTAAGTCCATTGAAAAAGACCTGAAGGCGATAAGGAAGTTCATGCGTCGACTTTCGAAGGGTAAATTCGTCGACGCCAGCAAGTATATAGAACCAGATGACGGTACAGACGATGGTGGTGGTGGATCTCGACCACATTCGAAAGGTCAGGATGATGGTGGTGGTCCTCCATCCGGGTCACAAGCAAAAGCAAATGACAACACCCCAATGGCTGACCATGCGGATCCGATGGATACAACAGAACAACATGGTCGAGTCGAGGAAGTAAATGACCCGATAGAGGGTGTGGGGAAAGACGTACAGATGGAGGTAACTGAAATAAGAGAACATGAAGTAATTGAAATAGGCGAACATGTAGATGCCCCGATAGAGGGTGTGGGAAAGGATATGCCTGTTGTCGAAAGTCAAAATTCTCTGGGTGTCCAGTCCATTTCTGAACAGAACGAGCCGATAGAAAGACGGGGGACTCGTAAGAGGAAGACTGCATGGAAGTTGAGAAGTCCATGGAAAGACACACGGGAAGACCAAGACACAGTTCGGAAAACTGCGTATGCTGTCCGAGACAAGACGTGGTTTCGTGATCTTATCACTCCATCGAAATGGATGACCGATGAGGTGGTCGACTCGATTTTCATGTTCGTCCAAAAGAAATTGGGAGAACGACCAGATTTATGCCGCAGGAAGTTCACCATTGGGGACCTATGTAATTTCTTCAGACGCGAAGATGGCCTATACAAGGAAATGAGTAGCGGCATACATCCCCGAGACTTGACGTACGATTGGAGTAGCGCAGGCAATATCTTGAAGTACGGAATGGGCGAGCTTGCAGACCATAACATACCATGGAGCACGGTCGATTTGGTGTACATGCCGTATAACATCGGCGGGCTGCATTGGGTCCTCGTATGCATTGACCTCGAGGTGGGGGAGGTCGTTGTATCGGATTCCATGGTGGTGTTGAATAAGGACGAGGTGGTTGAGAAGGAGTTAAGGGTCCTTTGCCAAGTCCTGCCAGCTCTGCTTTGGAAGATCGGGGTCATGGATGTGAGGAAGAATCTCTCCGTTCAAAGATGGCCTCTACGTCGGGAATTGTCAAGGCCGCAGCAGAAACGTAGTGGCGACTGCGGTATGTTTGTATGTAAATTTTTTGAATATGATGTAACTGGGTTGCCGTATGACACCCTAAGTCAGGATAATATGATTTATTTTCGGTGGCAATATGCTGTACAGATATGGGCGAACAGACCCTTCTTCTAG

Coding sequence (CDS)

ATGGACAACTTATTCATTTACTTTCTTTTGCGGGAAGTGAATGAGCCTAGGATTGATGTTATTAGCTTTGAGATTCTGGGGGAGAAAGTTTCATTTGGTCGGAGGGAATTTGACCTCATCACTGGAATTAGGCATAGGACCCAACATATTAGGGGTAATGTATCTAGTACTAGACTGAGAAGACTGTACCTTGACGATAGCATCAGCATGAAAGGGTTTGAACTAGATAAATTATTTCCTACCATTAATTTTGAGAGCGACGAGGATGCTGTGAAGATGGCTATATTTTATTTCATTGAGTTGGCTATGATGGGGAGGGAGAGAAAGCAGCAGATGGACACTAGCCTGCTCGGCATTATTGATGATTGGCAAAGGTTTTGTAATGAGGACTGGAGTAAGCTAATTTTTGATAAGACCATAAAGGGACTCAAGAAGGCAGTAGGTGGGAAGGCAGTGTCCTATAAAGAGAGGACAGATGGCAAACAGGAAACGTACAGTCTGTATGGCTTCCCATACGCGTTTCAGGTATGGACATACGAGACTGTATCTTCGTTGACCGGGCGTGTGGCTAATCGCTTGAATGACAATGCCATTCCACGCATATTAAGATGGTCATGTAGCCACTCACCTACACTTGCAGCGCTGAGTCGGGAGGTGTTTTCTTCAGATATGGCTAGGGTCACAACTGAACTTGTGGCCTCAGAAGAGGAGATCCAATTTATGGATCGTGTGATGCAGCCACCTCGAGCCTCATCTCCACCTCCACCTCCACCTCCACCTCCACCTCCACCTCCACCCCCAGTAGCTTTGGAAGATATTCCAGATGAAGATACTGTTGTTGAGGATCTCGGGACTGAGAATCCAAATGAAGTGGTGGAAGGTGTTGGGACGTCTGTTACGAATGACAGAGTCTGCAAGAGGTGCAAAGTCCTCGAAGACGAGATCAAAGTGATTAAAGAAGATGTGAAGGAGATTAAGGAGGATGTGAAAGTCATTAAGTCCATTGAAAAAGACCTGAAGGCGATAAGGAAGTTCATGCGTCGACTTTCGAAGGGTAAATTCGTCGACGCCAGCAAGTATATAGAACCAGATGACGGTACAGACGATGGTGGTGGTGGATCTCGACCACATTCGAAAGGTCAGGATGATGGTGGTGGTCCTCCATCCGGGTCACAAGCAAAAGCAAATGACAACACCCCAATGGCTGACCATGCGGATCCGATGGATACAACAGAACAACATGGTCGAGTCGAGGAAGTAAATGACCCGATAGAGGGTGTGGGGAAAGACGTACAGATGGAGGTAACTGAAATAAGAGAACATGAAGTAATTGAAATAGGCGAACATGTAGATGCCCCGATAGAGGGTGTGGGAAAGGATATGCCTGTTGTCGAAAGTCAAAATTCTCTGGGTGTCCAGTCCATTTCTGAACAGAACGAGCCGATAGAAAGACGGGGGACTCGTAAGAGGAAGACTGCATGGAAGTTGAGAAGTCCATGGAAAGACACACGGGAAGACCAAGACACAGTTCGGAAAACTGCGTATGCTGTCCGAGACAAGACGTGGTTTCGTGATCTTATCACTCCATCGAAATGGATGACCGATGAGGTGGTCGACTCGATTTTCATGTTCGTCCAAAAGAAATTGGGAGAACGACCAGATTTATGCCGCAGGAAGTTCACCATTGGGGACCTATGTAATTTCTTCAGACGCGAAGATGGCCTATACAAGGAAATGAGTAGCGGCATACATCCCCGAGACTTGACGTACGATTGGAGTAGCGCAGGCAATATCTTGAAGTACGGAATGGGCGAGCTTGCAGACCATAACATACCATGGAGCACGGTCGATTTGGTGTACATGCCGTATAACATCGGCGGGCTGCATTGGGTCCTCGTATGCATTGACCTCGAGGTGGGGGAGGTCGTTGTATCGGATTCCATGGTGGTGTTGAATAAGGACGAGGTGGTTGAGAAGGAGTTAAGGGTCCTTTGCCAAGTCCTGCCAGCTCTGCTTTGGAAGATCGGGGTCATGGATGTGAGGAAGAATCTCTCCGTTCAAAGATGGCCTCTACGTCGGGAATTGTCAAGGCCGCAGCAGAAACGTAGTGGCGACTGCGGTATGTTTGTATGTAAATTTTTTGAATATGATGTAACTGGGTTGCCGTATGACACCCTAAGTCAGGATAATATGATTTATTTTCGGTGGCAATATGCTGTACAGATATGGGCGAACAGACCCTTCTTCTAG

Protein sequence

MDNLFIYFLLREVNEPRIDVISFEILGEKVSFGRREFDLITGIRHRTQHIRGNVSSTRLRRLYLDDSISMKGFELDKLFPTINFESDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIIDDWQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYETVSSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDMARVTTELVASEEEIQFMDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDLGTENPNEVVEGVGTSVTNDRVCKRCKVLEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSKGKFVDASKYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTTEQHGRVEEVNDPIEGVGKDVQMEVTEIREHEVIEIGEHVDAPIEGVGKDMPVVESQNSLGVQSISEQNEPIERRGTRKRKTAWKLRSPWKDTREDQDTVRKTAYAVRDKTWFRDLITPSKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGDLCNFFRREDGLYKEMSSGIHPRDLTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVVSDSMVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQRWPLRRELSRPQQKRSGDCGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF
Homology
BLAST of Spg032334 vs. NCBI nr
Match: KAA0053486.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 269.2 bits (687), Expect = 1.0e-67
Identity = 224/770 (29.09%), Postives = 370/770 (48.05%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKV-SFGRREFDLITGIRHRTQHIRGNVSSTRLRRL 63
           L  Y LLREV +   D ISF +LG+ V +FGRREF++ITG+    +     V ++RL   
Sbjct: 69  LIHYLLLREVEDEGKDSISF-LLGDVVCTFGRREFNIITGLWGPKEDYIQLVGNSRLLEK 128

Query: 64  YLDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIID 123
           +  D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I D
Sbjct: 129 FFKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIAD 188

Query: 124 DWQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYET 183
           DW  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE+
Sbjct: 189 DWNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYES 248

Query: 184 VSSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQ 243
           + ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++
Sbjct: 249 IPTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLK 308

Query: 244 FMDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGT 303
                +     SS                         T+++    G++   EVV     
Sbjct: 309 IASGELFENFRSS-------------------------TIIQSKNGGSKRVREVVNDEDD 368

Query: 304 SVTNDRVCKRCKV------LEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSK 363
              +++   + K+      LED + V++  +  IK D+  +K +   +  I K +    K
Sbjct: 369 FKKSEKQKSKIKMKKATRNLEDRVAVVEGQLNSIKSDIDELKGM---MSTILKHIGLQRK 428

Query: 364 GKFVDASKYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTT 423
           G   D        D T +        ++  D GG P      K +++  +    D  D  
Sbjct: 429 GDEGDHKVSEGLVDHTLESKDVDNAKTEDVDTGGTPNWLRMPKEDEHIEVKKKGDE-DVL 488

Query: 424 EQHGRVEEVNDPIEGVGKDVQMEVTEI------REHEVIEIGEHVDAPIEGVGKDMPVVE 483
           E+   +  + +PI+ V  +   +VTEI      R H      +     +      +P   
Sbjct: 489 EKKVDI-GLEEPIDVVDDE---DVTEIEPFLTQRPHVRPARRKRASVYLSTPFTTLP--- 548

Query: 484 SQNSLGVQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWF 543
            + SL   + + + E I      K       +LR+ W   +   D +R+T +  + K +F
Sbjct: 549 -KRSLTSTTSTSEYEAIVYDPMHKILDVHLDRLRA-WITDKRTNDELRETFHGKKSKAFF 608

Query: 544 RDLITPSKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSS 603
           RDL    +W++DE +D+ F+F+  K+        + FT  D         +  LYKE   
Sbjct: 609 RDLFMCRRWLSDEHLDAFFLFICLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIK 668

Query: 604 GIHPRDLTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGE 663
              P    +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +
Sbjct: 669 ENRP----FDWDEEYRLVDYVVGSKEDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQ 728

Query: 664 VVVSDSMVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQ 723
           V V DS+  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P 
Sbjct: 729 VKVWDSLPSLTTAEDMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPL 788

Query: 724 QKRSGDCGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           Q+ + DCG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 QRNNSDCGVFTIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 791

BLAST of Spg032334 vs. NCBI nr
Match: KAA0054867.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 267.7 bits (683), Expect = 3.0e-67
Identity = 218/764 (28.53%), Postives = 368/764 (48.17%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKVSFGRREFDLITGIRHRTQHIRGNVSSTRLRRLY 63
           L  Y LLREV +   D ISF + G   +FGRREF++ITG+    +     V ++RL   +
Sbjct: 69  LIHYLLLREVEDEGKDSISFLLGGVVCTFGRREFNIITGLWGPKEDYIQLVGNSRLLEKF 128

Query: 64  LDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIIDD 123
             D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I DD
Sbjct: 129 FKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIADD 188

Query: 124 WQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYETV 183
           W  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE++
Sbjct: 189 WNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYESI 248

Query: 184 SSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQF 243
            ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++ 
Sbjct: 249 PTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLKI 308

Query: 244 MDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGTS 303
               +     SS                         T+++    G++   EV       
Sbjct: 309 ASGELFENFRSS-------------------------TIIQSKNGGSKRVREV------- 368

Query: 304 VTNDRVCKRCKVLEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSKGKFVDAS 363
           V ++   K+ K  + +IK +K+ ++ +++ V V   +E  L  I+  +  L KG      
Sbjct: 369 VNDEDELKKSKKQKSKIK-MKKAIRNLQDRVAV---VEGQLNTIKSDIDEL-KGLMSTIL 428

Query: 364 KYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTTEQHGRVE 423
           K+I      D+   G    S+G +D             ++  +    D  D  E+   + 
Sbjct: 429 KHIGLQRKGDE---GDHKVSEGLED-------------EHIEVKKKGDE-DVLEKKVDI- 488

Query: 424 EVNDPIEGVGKDVQMEV-------TEIREHEVIEIGEHVDAPIEGVGKDMPVVESQNSLG 483
            + +PI+ V  +  +E+         +R         ++  P   + K         SL 
Sbjct: 489 GLEEPIDVVDDEDVIEIEPFLTQRPHVRPARRKRASVYLSTPFTTLPK--------RSLT 548

Query: 484 VQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWFRDLITP 543
             + + ++E I      K       +LR+ W   +   D +R+T +  + K +FRDL   
Sbjct: 549 STTSTSESEAIVYDPMHKILDVHLDRLRA-WITDKRTDDELRETFHGKKSKAFFRDLFMC 608

Query: 544 SKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSSGIHPRD 603
            +W++DE +D++F+F++ K+        + FT  D         +  LYKE      P  
Sbjct: 609 RRWLSDEHLDALFLFIRLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKENRP-- 668

Query: 604 LTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVVSDS 663
             +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +V V DS
Sbjct: 669 --FDWDEEYRLVDYVVGSKVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDS 728

Query: 664 MVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQQKRSGD 723
           +  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P Q+ + D
Sbjct: 729 LPSLTTAEEMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNCD 760

Query: 724 CGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           CG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 CGVFAIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 760

BLAST of Spg032334 vs. NCBI nr
Match: TYJ95796.1 (Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 267.3 bits (682), Expect = 3.9e-67
Identity = 217/764 (28.40%), Postives = 368/764 (48.17%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKVSFGRREFDLITGIRHRTQHIRGNVSSTRLRRLY 63
           L  Y LLREV +   D ISF + G   +FGRREF+++TG+    +     V ++RL   +
Sbjct: 69  LIHYLLLREVEDEGKDSISFLLGGVVCTFGRREFNIVTGLWGPKEDYIQLVGNSRLLEKF 128

Query: 64  LDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIIDD 123
             D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I DD
Sbjct: 129 FKDKDCVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIADD 188

Query: 124 WQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYETV 183
           W  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE++
Sbjct: 189 WNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYESI 248

Query: 184 SSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQF 243
            ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++ 
Sbjct: 249 PTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLKI 308

Query: 244 MDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGTS 303
               +     SS                         T+++    G++   EV       
Sbjct: 309 ASGELFENFRSS-------------------------TIIQSKNGGSKRVREV------- 368

Query: 304 VTNDRVCKRCKVLEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSKGKFVDAS 363
           V ++   K+ K  + +IK +K+ ++ +++ V V   +E  L  I+  +  L KG      
Sbjct: 369 VNDEDELKKSKKQKSKIK-MKKAIRNLQDRVAV---VEGQLNTIKSDIDEL-KGLMSTIL 428

Query: 364 KYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTTEQHGRVE 423
           K+I      D+   G    S+G +D             ++  +    D  D  E+   + 
Sbjct: 429 KHIGLQRKGDE---GDHKVSEGLED-------------EHIEVKKKGDE-DVLEKKVDI- 488

Query: 424 EVNDPIEGVGKDVQMEV-------TEIREHEVIEIGEHVDAPIEGVGKDMPVVESQNSLG 483
            + +PI+ V  +  +E+         +R         ++  P   + K         SL 
Sbjct: 489 GLEEPIDVVDDEDVIEIEPFLTQRPHVRPARRKRASVYLSTPFTTLPK--------RSLT 548

Query: 484 VQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWFRDLITP 543
             + + ++E I      K       +LR+ W   +   D +R+T +  + K +FRDL   
Sbjct: 549 STTSTSESEAIVYDPMHKILDVHLDRLRA-WITDKRTDDELRETFHGKKSKAFFRDLFMC 608

Query: 544 SKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSSGIHPRD 603
            +W++DE +D++F+F++ K+        + FT  D         +  LYKE      P  
Sbjct: 609 RRWLSDEHLDALFLFIRLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKENRP-- 668

Query: 604 LTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVVSDS 663
             +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +V V DS
Sbjct: 669 --FDWDEEYRLVDYVVGSKVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDS 728

Query: 664 MVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQQKRSGD 723
           +  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P Q+ + D
Sbjct: 729 LPSLTTAEEMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNCD 760

Query: 724 CGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           CG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 CGVFAIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 760

BLAST of Spg032334 vs. NCBI nr
Match: TYK23325.1 (Ulp1-like peptidase [Cucumis melo var. makuwa] >TYK25950.1 Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 267.3 bits (682), Expect = 3.9e-67
Identity = 217/764 (28.40%), Postives = 368/764 (48.17%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKVSFGRREFDLITGIRHRTQHIRGNVSSTRLRRLY 63
           L  Y LLREV +   D ISF + G   +FGRREF+++TG+    +     V ++RL   +
Sbjct: 69  LIHYLLLREVEDEGKDSISFLLGGVVCTFGRREFNIVTGLWGPKEDYIQLVGNSRLLEKF 128

Query: 64  LDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIIDD 123
             D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I DD
Sbjct: 129 FKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIADD 188

Query: 124 WQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYETV 183
           W  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE++
Sbjct: 189 WNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYESI 248

Query: 184 SSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQF 243
            ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++ 
Sbjct: 249 PTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLKI 308

Query: 244 MDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGTS 303
               +     SS                         T+++    G++   EV       
Sbjct: 309 ASGELFENFRSS-------------------------TIIQSKNGGSKRVREV------- 368

Query: 304 VTNDRVCKRCKVLEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSKGKFVDAS 363
           V ++   K+ K  + +IK +K+ ++ +++ V V   +E  L  I+  +  L KG      
Sbjct: 369 VNDEDELKKSKKQKSKIK-MKKAIRNLQDRVAV---VEGQLNTIKSDIDEL-KGLMSTIL 428

Query: 364 KYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTTEQHGRVE 423
           K+I      D+   G    S+G +D             ++  +    D  D  E+   + 
Sbjct: 429 KHIGLQRKGDE---GDHKVSEGLED-------------EHIEVKKKGDE-DVLEKKVDI- 488

Query: 424 EVNDPIEGVGKDVQMEV-------TEIREHEVIEIGEHVDAPIEGVGKDMPVVESQNSLG 483
            + +PI+ V  +  +E+         +R         ++  P   + K         SL 
Sbjct: 489 GLEEPIDVVDDEDVIEIEPFLTQRPHVRPARRKRASVYLSTPFTTLPK--------RSLT 548

Query: 484 VQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWFRDLITP 543
             + + ++E I      K       +LR+ W   +   D +R+T +  + K +FRDL   
Sbjct: 549 STTSTSESEAIVYDPMHKILDVHLDRLRA-WITDKRTDDELRETFHGKKSKAFFRDLFMC 608

Query: 544 SKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSSGIHPRD 603
            +W++DE +D++F+F++ K+        + FT  D         +  LYKE      P  
Sbjct: 609 RRWLSDEHLDALFLFIRLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKENRP-- 668

Query: 604 LTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVVSDS 663
             +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +V V DS
Sbjct: 669 --FDWDEEYRLVDYVVGSKVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDS 728

Query: 664 MVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQQKRSGD 723
           +  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P Q+ + D
Sbjct: 729 LPSLTTAEEMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNCD 760

Query: 724 CGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           CG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 CGVFAIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 760

BLAST of Spg032334 vs. NCBI nr
Match: KAA0055496.1 (Ulp1-like peptidase [Cucumis melo var. makuwa] >TYK14349.1 Ulp1-like peptidase [Cucumis melo var. makuwa] >TYK18723.1 Ulp1-like peptidase [Cucumis melo var. makuwa] >TYK28419.1 Ulp1-like peptidase [Cucumis melo var. makuwa])

HSP 1 Score: 264.6 bits (675), Expect = 2.6e-66
Identity = 216/764 (28.27%), Postives = 367/764 (48.04%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKVSFGRREFDLITGIRHRTQHIRGNVSSTRLRRLY 63
           L  Y LLREV +   D ISF + G   +FGRREF+++TG+    +       ++RL   +
Sbjct: 69  LIHYLLLREVEDEGKDSISFLLGGVVCTFGRREFNIVTGLWGPKEDYIQLGGNSRLLEKF 128

Query: 64  LDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIIDD 123
             D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I DD
Sbjct: 129 FKDKDCVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIADD 188

Query: 124 WQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYETV 183
           W  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE++
Sbjct: 189 WNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYESI 248

Query: 184 SSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQF 243
            ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++ 
Sbjct: 249 PTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLKI 308

Query: 244 MDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGTS 303
               +     SS                         T+++    G++   EV       
Sbjct: 309 ASGELFENFRSS-------------------------TIIQSKNGGSKRVREV------- 368

Query: 304 VTNDRVCKRCKVLEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSKGKFVDAS 363
           V ++   K+ K  + +IK +K+ ++ +++ V V   +E  L  I+  +  L KG      
Sbjct: 369 VNDEDELKKSKKQKSKIK-MKKAIRNLQDRVAV---VEGQLNTIKSDIDEL-KGLMSTIL 428

Query: 364 KYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTTEQHGRVE 423
           K+I      D+   G    S+G +D             ++  +    D  D  E+   + 
Sbjct: 429 KHIGLQRKGDE---GDHKVSEGLED-------------EHIEVKKKGDE-DVLEKKVDI- 488

Query: 424 EVNDPIEGVGKDVQMEV-------TEIREHEVIEIGEHVDAPIEGVGKDMPVVESQNSLG 483
            + +PI+ V  +  +E+         +R         ++  P   + K         SL 
Sbjct: 489 GLEEPIDVVDDEDVIEIEPFLTQRPHVRPARRKRASVYLSTPFTTLPK--------RSLT 548

Query: 484 VQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWFRDLITP 543
             + + ++E I      K       +LR+ W   +   D +R+T +  + K +FRDL   
Sbjct: 549 STTSTSESEAIVYDPMHKILDVHLDRLRA-WITDKRTDDELRETFHGKKSKAFFRDLFMC 608

Query: 544 SKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSSGIHPRD 603
            +W++DE +D++F+F++ K+        + FT  D         +  LYKE      P  
Sbjct: 609 RRWLSDEHLDALFLFIRLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKENRP-- 668

Query: 604 LTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVVSDS 663
             +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +V V DS
Sbjct: 669 --FDWDEEYRLVDYVVGSKVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDS 728

Query: 664 MVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQQKRSGD 723
           +  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P Q+ + D
Sbjct: 729 LPSLTTAEEMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNCD 760

Query: 724 CGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           CG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 CGVFAIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 760

BLAST of Spg032334 vs. ExPASy Swiss-Prot
Match: O65278 (Putative ubiquitin-like-specific protease 1B OS=Arabidopsis thaliana OX=3702 GN=ULP1B PE=5 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 9.0e-06
Identity = 52/218 (23.85%), Postives = 96/218 (44.04%), Query Frame = 0

Query: 527 ITPSKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGDLCNFFRREDGLYKEMSSGIHPR 586
           + P++W+ D+V +     ++++    P   ++ F     C+FF      Y ++ SG    
Sbjct: 149 LRPNQWLNDDVTNLYLELLKERQTRDP---QKYFK----CHFF--NTFFYVKLVSG---- 208

Query: 587 DLTYDWSSAGN-ILKYGMG-ELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVV 646
              Y++ +      K  +G +L D        D++++P +I  +HW L  I+    + V 
Sbjct: 209 -SGYNYKAVSRWTTKRKLGYDLID-------CDIIFVPIHI-DIHWTLGVINNRERKFVY 268

Query: 647 SDSMVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQRWPLRRELSRPQQKRSG 706
            DS+       ++    + L   +            +KN+ V  W +     RPQQ+   
Sbjct: 269 LDSLFTGVGHTILNAMAKYLVDEVK--------QKSQKNIDVSSWGMEYVEERPQQQNGY 328

Query: 707 DCGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQI 743
           DCGMF+ K+ ++   GL     SQ +M YFR + A +I
Sbjct: 329 DCGMFMLKYIDFYSRGLSLQ-FSQKDMPYFRLRTAKEI 335

BLAST of Spg032334 vs. ExPASy TrEMBL
Match: A0A5A7UGY3 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold190G00350 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 5.0e-68
Identity = 224/770 (29.09%), Postives = 370/770 (48.05%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKV-SFGRREFDLITGIRHRTQHIRGNVSSTRLRRL 63
           L  Y LLREV +   D ISF +LG+ V +FGRREF++ITG+    +     V ++RL   
Sbjct: 69  LIHYLLLREVEDEGKDSISF-LLGDVVCTFGRREFNIITGLWGPKEDYIQLVGNSRLLEK 128

Query: 64  YLDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIID 123
           +  D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I D
Sbjct: 129 FFKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIAD 188

Query: 124 DWQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYET 183
           DW  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE+
Sbjct: 189 DWNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYES 248

Query: 184 VSSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQ 243
           + ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++
Sbjct: 249 IPTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLK 308

Query: 244 FMDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGT 303
                +     SS                         T+++    G++   EVV     
Sbjct: 309 IASGELFENFRSS-------------------------TIIQSKNGGSKRVREVVNDEDD 368

Query: 304 SVTNDRVCKRCKV------LEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSK 363
              +++   + K+      LED + V++  +  IK D+  +K +   +  I K +    K
Sbjct: 369 FKKSEKQKSKIKMKKATRNLEDRVAVVEGQLNSIKSDIDELKGM---MSTILKHIGLQRK 428

Query: 364 GKFVDASKYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTT 423
           G   D        D T +        ++  D GG P      K +++  +    D  D  
Sbjct: 429 GDEGDHKVSEGLVDHTLESKDVDNAKTEDVDTGGTPNWLRMPKEDEHIEVKKKGDE-DVL 488

Query: 424 EQHGRVEEVNDPIEGVGKDVQMEVTEI------REHEVIEIGEHVDAPIEGVGKDMPVVE 483
           E+   +  + +PI+ V  +   +VTEI      R H      +     +      +P   
Sbjct: 489 EKKVDI-GLEEPIDVVDDE---DVTEIEPFLTQRPHVRPARRKRASVYLSTPFTTLP--- 548

Query: 484 SQNSLGVQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWF 543
            + SL   + + + E I      K       +LR+ W   +   D +R+T +  + K +F
Sbjct: 549 -KRSLTSTTSTSEYEAIVYDPMHKILDVHLDRLRA-WITDKRTNDELRETFHGKKSKAFF 608

Query: 544 RDLITPSKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSS 603
           RDL    +W++DE +D+ F+F+  K+        + FT  D         +  LYKE   
Sbjct: 609 RDLFMCRRWLSDEHLDAFFLFICLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIK 668

Query: 604 GIHPRDLTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGE 663
              P    +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +
Sbjct: 669 ENRP----FDWDEEYRLVDYVVGSKEDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQ 728

Query: 664 VVVSDSMVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQ 723
           V V DS+  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P 
Sbjct: 729 VKVWDSLPSLTTAEDMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPL 788

Query: 724 QKRSGDCGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           Q+ + DCG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 QRNNSDCGVFTIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 791

BLAST of Spg032334 vs. ExPASy TrEMBL
Match: A0A5A7UJU4 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G00840 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.5e-67
Identity = 218/764 (28.53%), Postives = 368/764 (48.17%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKVSFGRREFDLITGIRHRTQHIRGNVSSTRLRRLY 63
           L  Y LLREV +   D ISF + G   +FGRREF++ITG+    +     V ++RL   +
Sbjct: 69  LIHYLLLREVEDEGKDSISFLLGGVVCTFGRREFNIITGLWGPKEDYIQLVGNSRLLEKF 128

Query: 64  LDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIIDD 123
             D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I DD
Sbjct: 129 FKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIADD 188

Query: 124 WQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYETV 183
           W  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE++
Sbjct: 189 WNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYESI 248

Query: 184 SSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQF 243
            ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++ 
Sbjct: 249 PTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLKI 308

Query: 244 MDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGTS 303
               +     SS                         T+++    G++   EV       
Sbjct: 309 ASGELFENFRSS-------------------------TIIQSKNGGSKRVREV------- 368

Query: 304 VTNDRVCKRCKVLEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSKGKFVDAS 363
           V ++   K+ K  + +IK +K+ ++ +++ V V   +E  L  I+  +  L KG      
Sbjct: 369 VNDEDELKKSKKQKSKIK-MKKAIRNLQDRVAV---VEGQLNTIKSDIDEL-KGLMSTIL 428

Query: 364 KYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTTEQHGRVE 423
           K+I      D+   G    S+G +D             ++  +    D  D  E+   + 
Sbjct: 429 KHIGLQRKGDE---GDHKVSEGLED-------------EHIEVKKKGDE-DVLEKKVDI- 488

Query: 424 EVNDPIEGVGKDVQMEV-------TEIREHEVIEIGEHVDAPIEGVGKDMPVVESQNSLG 483
            + +PI+ V  +  +E+         +R         ++  P   + K         SL 
Sbjct: 489 GLEEPIDVVDDEDVIEIEPFLTQRPHVRPARRKRASVYLSTPFTTLPK--------RSLT 548

Query: 484 VQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWFRDLITP 543
             + + ++E I      K       +LR+ W   +   D +R+T +  + K +FRDL   
Sbjct: 549 STTSTSESEAIVYDPMHKILDVHLDRLRA-WITDKRTDDELRETFHGKKSKAFFRDLFMC 608

Query: 544 SKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSSGIHPRD 603
            +W++DE +D++F+F++ K+        + FT  D         +  LYKE      P  
Sbjct: 609 RRWLSDEHLDALFLFIRLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKENRP-- 668

Query: 604 LTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVVSDS 663
             +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +V V DS
Sbjct: 669 --FDWDEEYRLVDYVVGSKVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDS 728

Query: 664 MVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQQKRSGD 723
           +  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P Q+ + D
Sbjct: 729 LPSLTTAEEMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNCD 760

Query: 724 CGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           CG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 CGVFAIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 760

BLAST of Spg032334 vs. ExPASy TrEMBL
Match: A0A5D3DIC1 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G003860 PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.9e-67
Identity = 217/764 (28.40%), Postives = 368/764 (48.17%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKVSFGRREFDLITGIRHRTQHIRGNVSSTRLRRLY 63
           L  Y LLREV +   D ISF + G   +FGRREF+++TG+    +     V ++RL   +
Sbjct: 69  LIHYLLLREVEDEGKDSISFLLGGVVCTFGRREFNIVTGLWGPKEDYIQLVGNSRLLEKF 128

Query: 64  LDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIIDD 123
             D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I DD
Sbjct: 129 FKDKECVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIADD 188

Query: 124 WQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYETV 183
           W  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE++
Sbjct: 189 WNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYESI 248

Query: 184 SSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQF 243
            ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++ 
Sbjct: 249 PTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLKI 308

Query: 244 MDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGTS 303
               +     SS                         T+++    G++   EV       
Sbjct: 309 ASGELFENFRSS-------------------------TIIQSKNGGSKRVREV------- 368

Query: 304 VTNDRVCKRCKVLEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSKGKFVDAS 363
           V ++   K+ K  + +IK +K+ ++ +++ V V   +E  L  I+  +  L KG      
Sbjct: 369 VNDEDELKKSKKQKSKIK-MKKAIRNLQDRVAV---VEGQLNTIKSDIDEL-KGLMSTIL 428

Query: 364 KYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTTEQHGRVE 423
           K+I      D+   G    S+G +D             ++  +    D  D  E+   + 
Sbjct: 429 KHIGLQRKGDE---GDHKVSEGLED-------------EHIEVKKKGDE-DVLEKKVDI- 488

Query: 424 EVNDPIEGVGKDVQMEV-------TEIREHEVIEIGEHVDAPIEGVGKDMPVVESQNSLG 483
            + +PI+ V  +  +E+         +R         ++  P   + K         SL 
Sbjct: 489 GLEEPIDVVDDEDVIEIEPFLTQRPHVRPARRKRASVYLSTPFTTLPK--------RSLT 548

Query: 484 VQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWFRDLITP 543
             + + ++E I      K       +LR+ W   +   D +R+T +  + K +FRDL   
Sbjct: 549 STTSTSESEAIVYDPMHKILDVHLDRLRA-WITDKRTDDELRETFHGKKSKAFFRDLFMC 608

Query: 544 SKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSSGIHPRD 603
            +W++DE +D++F+F++ K+        + FT  D         +  LYKE      P  
Sbjct: 609 RRWLSDEHLDALFLFIRLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKENRP-- 668

Query: 604 LTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVVSDS 663
             +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +V V DS
Sbjct: 669 --FDWDEEYRLVDYVVGSKVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDS 728

Query: 664 MVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQQKRSGD 723
           +  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P Q+ + D
Sbjct: 729 LPSLTTAEEMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNCD 760

Query: 724 CGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           CG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 CGVFAIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 760

BLAST of Spg032334 vs. ExPASy TrEMBL
Match: A0A5D3BA80 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G00730 PE=3 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 1.9e-67
Identity = 217/764 (28.40%), Postives = 368/764 (48.17%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKVSFGRREFDLITGIRHRTQHIRGNVSSTRLRRLY 63
           L  Y LLREV +   D ISF + G   +FGRREF+++TG+    +     V ++RL   +
Sbjct: 69  LIHYLLLREVEDEGKDSISFLLGGVVCTFGRREFNIVTGLWGPKEDYIQLVGNSRLLEKF 128

Query: 64  LDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIIDD 123
             D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I DD
Sbjct: 129 FKDKDCVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIADD 188

Query: 124 WQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYETV 183
           W  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE++
Sbjct: 189 WNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYESI 248

Query: 184 SSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQF 243
            ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++ 
Sbjct: 249 PTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLKI 308

Query: 244 MDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGTS 303
               +     SS                         T+++    G++   EV       
Sbjct: 309 ASGELFENFRSS-------------------------TIIQSKNGGSKRVREV------- 368

Query: 304 VTNDRVCKRCKVLEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSKGKFVDAS 363
           V ++   K+ K  + +IK +K+ ++ +++ V V   +E  L  I+  +  L KG      
Sbjct: 369 VNDEDELKKSKKQKSKIK-MKKAIRNLQDRVAV---VEGQLNTIKSDIDEL-KGLMSTIL 428

Query: 364 KYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTTEQHGRVE 423
           K+I      D+   G    S+G +D             ++  +    D  D  E+   + 
Sbjct: 429 KHIGLQRKGDE---GDHKVSEGLED-------------EHIEVKKKGDE-DVLEKKVDI- 488

Query: 424 EVNDPIEGVGKDVQMEV-------TEIREHEVIEIGEHVDAPIEGVGKDMPVVESQNSLG 483
            + +PI+ V  +  +E+         +R         ++  P   + K         SL 
Sbjct: 489 GLEEPIDVVDDEDVIEIEPFLTQRPHVRPARRKRASVYLSTPFTTLPK--------RSLT 548

Query: 484 VQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWFRDLITP 543
             + + ++E I      K       +LR+ W   +   D +R+T +  + K +FRDL   
Sbjct: 549 STTSTSESEAIVYDPMHKILDVHLDRLRA-WITDKRTDDELRETFHGKKSKAFFRDLFMC 608

Query: 544 SKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSSGIHPRD 603
            +W++DE +D++F+F++ K+        + FT  D         +  LYKE      P  
Sbjct: 609 RRWLSDEHLDALFLFIRLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKENRP-- 668

Query: 604 LTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVVSDS 663
             +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +V V DS
Sbjct: 669 --FDWDEEYRLVDYVVGSKVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDS 728

Query: 664 MVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQQKRSGD 723
           +  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P Q+ + D
Sbjct: 729 LPSLTTAEEMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNCD 760

Query: 724 CGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           CG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 CGVFAIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 760

BLAST of Spg032334 vs. ExPASy TrEMBL
Match: A0A5D3DYH3 (Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00240 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.2e-66
Identity = 216/764 (28.27%), Postives = 367/764 (48.04%), Query Frame = 0

Query: 4   LFIYFLLREVNEPRIDVISFEILGEKVSFGRREFDLITGIRHRTQHIRGNVSSTRLRRLY 63
           L  Y LLREV +   D ISF + G   +FGRREF+++TG+    +       ++RL   +
Sbjct: 69  LIHYLLLREVEDEGKDSISFLLGGVVCTFGRREFNIVTGLWGPKEDYIQLGGNSRLLEKF 128

Query: 64  LDDSISMKGFELDKLFPTINFE-SDEDAVKMAIFYFIELAMMGRERKQQMDTSLLGIIDD 123
             D   +   +L+ +F  + +E  D+D VK+A+ YFIE++++G++R+ ++D     I DD
Sbjct: 129 FKDKDCVYVSDLEDIF--LEYEGDDDDIVKLALVYFIEISLLGKDRRTKVDIGFFKIADD 188

Query: 124 WQRFCNEDWSKLIFDKTIKGLKKAVGGKAVSYKERTDGKQETYSLYGFPYAFQVWTYETV 183
           W  F N DW +++F +T+  LK+A+  +    K+++  + + Y++ GFP+A QVW YE++
Sbjct: 189 WNSFNNYDWGRIVFVRTLSALKRALDKQYAKGKKKST-QTKKYTINGFPHALQVWAYESI 248

Query: 184 SSLTGRVANRLNDNAIPRILRWSCSHSPTLAALSREVFSSDM--ARVTTELVASEEEIQF 243
            ++ G   +++ND+AIPR+LRW C  SP    +S +VF S M   +   E+   EE+++ 
Sbjct: 249 PTIIGCGVDKVNDHAIPRMLRWVCQQSPKSQTIS-QVFDSPMFIIKAVIEMTPEEEQLKI 308

Query: 244 MDRVMQPPRASSPPPPPPPPPPPPPPPVALEDIPDEDTVVEDL--GTENPNEVVEGVGTS 303
               +     SS                         T+++    G++   EV       
Sbjct: 309 ASGELFENFRSS-------------------------TIIQSKNGGSKRVREV------- 368

Query: 304 VTNDRVCKRCKVLEDEIKVIKEDVKEIKEDVKVIKSIEKDLKAIRKFMRRLSKGKFVDAS 363
           V ++   K+ K  + +IK +K+ ++ +++ V V   +E  L  I+  +  L KG      
Sbjct: 369 VNDEDELKKSKKQKSKIK-MKKAIRNLQDRVAV---VEGQLNTIKSDIDEL-KGLMSTIL 428

Query: 364 KYIEPDDGTDDGGGGSRPHSKGQDDGGGPPSGSQAKANDNTPMADHADPMDTTEQHGRVE 423
           K+I      D+   G    S+G +D             ++  +    D  D  E+   + 
Sbjct: 429 KHIGLQRKGDE---GDHKVSEGLED-------------EHIEVKKKGDE-DVLEKKVDI- 488

Query: 424 EVNDPIEGVGKDVQMEV-------TEIREHEVIEIGEHVDAPIEGVGKDMPVVESQNSLG 483
            + +PI+ V  +  +E+         +R         ++  P   + K         SL 
Sbjct: 489 GLEEPIDVVDDEDVIEIEPFLTQRPHVRPARRKRASVYLSTPFTTLPK--------RSLT 548

Query: 484 VQSISEQNEPIERRGTRKRKTAW--KLRSPWKDTREDQDTVRKTAYAVRDKTWFRDLITP 543
             + + ++E I      K       +LR+ W   +   D +R+T +  + K +FRDL   
Sbjct: 549 STTSTSESEAIVYDPMHKILDVHLDRLRA-WITDKRTDDELRETFHGKKSKAFFRDLFMC 608

Query: 544 SKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGD--LCNFFRREDGLYKEMSSGIHPRD 603
            +W++DE +D++F+F++ K+        + FT  D         +  LYKE      P  
Sbjct: 609 RRWLSDEHLDALFLFIRLKIKAAGIPSSQNFTTADTIFMRILVSKWPLYKECIKENRP-- 668

Query: 604 LTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVVSDS 663
             +DW     ++ Y +G   D   PW++VD VY P+N+ G HWVL+C+DL   +V V DS
Sbjct: 669 --FDWDEEYRLVDYVVGSKVDFQDPWASVDYVYSPFNVHGNHWVLLCLDLVSCQVKVWDS 728

Query: 664 MVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQR--WPLRRELSRPQQKRSGD 723
           +  L   E +   L  + Q++P LL   G  D R   S  +  WP+    S P Q+ + D
Sbjct: 729 LPSLTTAEEMTNILLPIRQLVPKLLDSTGFFDRRGRSSTYKEPWPVVIVDSIPLQRNNCD 760

Query: 724 CGMFVCKFFEYDVTGLPYDTLSQDNMIYFRWQYAVQIWANRPFF 750
           CG+F  K+FEY   G+  DTL Q+NM YFR Q A Q+W N P +
Sbjct: 789 CGVFAIKYFEYIAAGVGLDTLCQENMSYFRKQLAFQLWTNTPMY 760

BLAST of Spg032334 vs. TAIR 10
Match: AT2G07240.1 (cysteine-type peptidases;cysteine-type peptidases )

HSP 1 Score: 62.8 bits (151), Expect = 1.4e-09
Identity = 50/185 (27.03%), Postives = 87/185 (47.03%), Query Frame = 0

Query: 525 DLITPSKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGDL-CNFFRREDGLYKEMSSGI 584
           D+I   K  + +V+D +  F  + L    D+   K  +  L   F  +   L+ + +  +
Sbjct: 653 DIIERKKHFSSKVMDVLIKF-SRHLLRTDDIDGEKLRVDVLGSKFVSQLTRLFPKFAKTL 712

Query: 585 HPRDLTYDWSSAGNILKYGMGELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVV 644
            P+D  +   SA   L  G GE ++    ++  D VYMP+N    HWV +C+DL+  ++ 
Sbjct: 713 PPKDFIF--PSALVDLLIGAGE-SNRVRLFAEADCVYMPFNFDKKHWVSLCVDLKAHKIT 772

Query: 645 VSDSMVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQRWPLRRELSRPQQKRS 704
           + DS + L +D  +  EL+ L  +LP L  +        ++S+Q + L R    PQ    
Sbjct: 773 ILDSNIQLRRDAALYAELQPLAAMLPYLFRQ--TSSSSGDISLQPFLLDRPHGIPQVSSP 831

Query: 705 GDCGM 709
            D G+
Sbjct: 833 FDSGV 831

BLAST of Spg032334 vs. TAIR 10
Match: AT4G00690.1 (UB-like protease 1B )

HSP 1 Score: 49.3 bits (116), Expect = 1.6e-05
Identity = 52/225 (23.11%), Postives = 97/225 (43.11%), Query Frame = 0

Query: 527 ITPSKWMTDEVVDSIFMFVQKKLGERPDLCRRKFTIGDLCNFFRREDGLYKEMSSGIHPR 586
           + P++W+ D+V +     ++++    P   ++ F     C+FF      Y ++ SG    
Sbjct: 149 LRPNQWLNDDVTNLYLELLKERQTRDP---QKYFK----CHFF--NTFFYVKLVSG---- 208

Query: 587 DLTYDWSSAGN-ILKYGMG-ELADHNIPWSTVDLVYMPYNIGGLHWVLVCIDLEVGEVVV 646
              Y++ +      K  +G +L D        D++++P +I  +HW L  I+    + V 
Sbjct: 209 -SGYNYKAVSRWTTKRKLGYDLID-------CDIIFVPIHI-DIHWTLGVINNRERKFVY 268

Query: 647 SDSMVVLNKDEVVEKELRVLCQVLPALLWKIGVMDVRKNLSVQRWPLRRELSRPQQKRSG 706
            DS+       ++    + L   +            +KN+ V  W +     RPQQ+   
Sbjct: 269 LDSLFTGVGHTILNAMAKYLVDEVK--------QKSQKNIDVSSWGMEYVEERPQQQNGY 328

Query: 707 DCGMFVCKFFEYDVTGLPY-------DTLSQDNMIYFRWQYAVQI 743
           DCGMF+ K+ ++   GL         D + +D M YFR + A +I
Sbjct: 329 DCGMFMLKYIDFYSRGLSLQFSQVIRDVIKKD-MPYFRLRTAKEI 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0053486.11.0e-6729.09Ulp1-like peptidase [Cucumis melo var. makuwa][more]
KAA0054867.13.0e-6728.53Ulp1-like peptidase [Cucumis melo var. makuwa][more]
TYJ95796.13.9e-6728.40Ulp1-like peptidase [Cucumis melo var. makuwa][more]
TYK23325.13.9e-6728.40Ulp1-like peptidase [Cucumis melo var. makuwa] >TYK25950.1 Ulp1-like peptidase [... [more]
KAA0055496.12.6e-6628.27Ulp1-like peptidase [Cucumis melo var. makuwa] >TYK14349.1 Ulp1-like peptidase [... [more]
Match NameE-valueIdentityDescription
O652789.0e-0623.85Putative ubiquitin-like-specific protease 1B OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A5A7UGY35.0e-6829.09Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold190G... [more]
A0A5A7UJU41.5e-6728.53Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold406G... [more]
A0A5D3DIC11.9e-6728.40Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G... [more]
A0A5D3BA801.9e-6728.40Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold110G... [more]
A0A5D3DYH31.2e-6628.27Ulp1-like peptidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G... [more]
Match NameE-valueIdentityDescription
AT2G07240.11.4e-0927.03cysteine-type peptidases;cysteine-type peptidases [more]
AT4G00690.11.6e-0523.11UB-like protease 1B [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 308..328
NoneNo IPR availableGENE3D3.40.395.10Adenoviral Proteinase; Chain Acoord: 476..746
e-value: 8.1E-29
score: 102.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 248..270
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..270
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 359..376
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 359..413
NoneNo IPR availablePANTHERPTHR12606SENTRIN/SUMO-SPECIFIC PROTEASEcoord: 369..745
NoneNo IPR availablePANTHERPTHR12606:SF109BNACNNG52470D PROTEINcoord: 369..745
NoneNo IPR availableSUPERFAMILY101447Formin homology 2 domain (FH2 domain)coord: 257..267
IPR003653Ulp1 protease family, C-terminal catalytic domainPFAMPF02902Peptidase_C48coord: 615..742
e-value: 1.0E-23
score: 84.3
IPR003653Ulp1 protease family, C-terminal catalytic domainPROSITEPS50600ULP_PROTEASEcoord: 500..717
score: 15.333185
IPR015410Domain of unknown function DUF1985PFAMPF09331DUF1985coord: 9..141
e-value: 8.1E-12
score: 45.3
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 525..744

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg032334.1Spg032334.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008234 cysteine-type peptidase activity