Spg032308 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg032308
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionGlyoxysomal processing protease, glyoxysomal
Locationscaffold2: 34899212 .. 34900981 (-)
RNA-Seq ExpressionSpg032308
SyntenySpg032308
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTGGCAAGATGGTATTCCTTTATGGGAAAAGAAATTCTGAACAGAGATTGGTTGTGTGCCATGGGGTAAAATAGTTGATTCCAAGACATTTATTTATTGGCATACTAATATAGTCAATTGGGATAATTATAGTCAATTGGGATAATTCTGCTTGTGAAGAAGCCTTCCATAATGCCAAAAGACGTTATTGTAATCTAAAGTAGGATTTGGGATTTTATGTGAAATTGTGTTTTACTTCAAGAAAAAATAGTTGTGGTTGCTTATGTGAAATTTCATTTTGAACTTCCTTTCACCATTCGATTTTCTTCCACTCGCTGAGACTTATGGTGGACATGGTAACCAGTTTTTATTGATTTCAGCATGTCTGAATGACATACTGGAATCTCACTGATGACTTTAGTAGTTGTTCTGCATATGACGGATCAAAGATACATGTTATTGGACACGGACTGTTGGGACCAAAATCTGGTATCTCTTTCTTCAAATTTATTGGTCTATTATTTTTGTCGTGTGATTTATTTTATTTTATTCAGTTTGGCATGTTATATTGGTATTAGAAAAAGTTATTGATAGAATCCAAACTATATTGCAATATTAAAGATAACCAAACGACAAAACAAACCAAGTGTTTCAGGTACTTGGACCCTCTTGAGATATCCCTCTCAAGTCCAAATAATGTCTGATTATCTTCTTCCCCACCCTCTATTAATCAACTATTATAACAAACTCCCTAACTAATTACTAATATACCCTTAATACCCTAATAACGCACTAATAGCATTGCTATCAGTTTTGCTCTAGGGGCAGGGATGAACTAGATGAAGGAACCTTTCTTTCTTACCCAGTTTAGACCATTTAAACAGTTTCAGCAACCATTCAGCAGTCATCATTATTATTTTTTAAAGCGTTCTTGATAACCAAAAAGAATTCCTTGGAAGGAAAAAGATGTATTATTTGCATTATATAGATAAAAGTTTAATTTCTTTTTTTTTTTCTTTTTTCCCCCTTTTTTGAATAATGGTTGTTGACCTTTTTATTTGAAGGAGGGGGTGACTAACTTTCCAGTGTCTTTTTTTTTCTTTTACATATAAATTAATTTACAAGTTGGCGTACCTTTTGCACTTCTTTCCACACATGCACAAATATGTTTATTTTTTAGATTATCTAGATCTAGTTTGAACTTTGTTAGGCTTCTCCCCATCTGTTTGCTCTGGTGTGGTGGCCAATGTGGTGAAAGCAAAGATTCCCTCTTCTTATCATCAAGGAGATTCATTAGAATATTTTCCTCCAATTCTCGAAACAACAGCTGTAGTGCATCCTGGTAGTAGTGGGGGTGCTGTTGTCAATTCAGAAGGCCGTATGATTGGACTTGTTACAAGGTATGGTACAACAAAATTACCTGTCCTCTCATCATTACTTAAGGGTTGTAAGGTTAATTTAGTTTTGTTTGTGGACGGCTATGCTTACAAAGATTGGTCTATCGTATTTGGTTGATGCTTTTGATTTTCCTGGAATCTTTGGTAGTTCATGACAATAGTCCATTAATGGTATCTCTTTATCCGTATACGCATCAGTCTGAATCAAGTTCAATAATATGTCTGTTTTTTCTAGTGATGTTAGTCTATGATTTCTTATACCGAGCCACCATTAGTTTATGGTGGATTTCGACTGAAAATTAATCATTTCTGTTTAATGTTGATTCTGTTCATGACAATAGTTTCAATGTTAAGCTCATTTTCTTTTAAGTGTAGGATGTGCTTCTTCTAGT

mRNA sequence

TTTTGGCAAGATGGCTTCTCCCCATCTGTTTGCTCTGGTGTGGTGGCCAATGTGGTGAAAGCAAAGATTCCCTCTTCTTATCATCAAGGAGATTCATTAGAATATTTTCCTCCAATTCTCGAAACAACAGCTGTAGTGCATCCTGGTAGTAGTGGGGGTGCTGTTGTCAATTCAGAAGGCCGTATGATTGGACTTGTTACAAGGTATGGTACAACAAAATTACCTGTCCTCTCATCATTACTTAAGGGTTGTAAGGTTAATTTAGTTTTGTTTGTGGACGGCTATGCTTACAAAGATTGGTCTATCGTATTTGGTTGA

Coding sequence (CDS)

TTTTGGCAAGATGGCTTCTCCCCATCTGTTTGCTCTGGTGTGGTGGCCAATGTGGTGAAAGCAAAGATTCCCTCTTCTTATCATCAAGGAGATTCATTAGAATATTTTCCTCCAATTCTCGAAACAACAGCTGTAGTGCATCCTGGTAGTAGTGGGGGTGCTGTTGTCAATTCAGAAGGCCGTATGATTGGACTTGTTACAAGGTATGGTACAACAAAATTACCTGTCCTCTCATCATTACTTAAGGGTTGTAAGGTTAATTTAGTTTTGTTTGTGGACGGCTATGCTTACAAAGATTGGTCTATCGTATTTGGTTGA

Protein sequence

FWQDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRMIGLVTRYGTTKLPVLSSLLKGCKVNLVLFVDGYAYKDWSIVFG
Homology
BLAST of Spg032308 vs. NCBI nr
Match: XP_031742825.1 (glyoxysomal processing protease, glyoxysomal isoform X6 [Cucumis sativus])

HSP 1 Score: 136.7 bits (343), Expect = 1.1e-28
Identity = 69/94 (73.40%), Postives = 75/94 (79.79%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + G SPSVCSGVV+NVVKAKIPSSYH+GDSLEYFP +LETTA VHPG SGGAVVNSEG M
Sbjct: 570 KSGLSPSVCSGVVSNVVKAKIPSSYHKGDSLEYFPAMLETTAAVHPGGSGGAVVNSEGHM 629

Query: 63  IGLVTRYGTTKLPVLSSLLKGCKVNLVLFVDGYA 97
           IGLVT         LSSLLK C+ NLVLFV  Y+
Sbjct: 630 IGLVTS--------LSSLLKACRTNLVLFVGSYS 655

BLAST of Spg032308 vs. NCBI nr
Match: XP_038881510.1 (glyoxysomal processing protease, glyoxysomal isoform X3 [Benincasa hispida])

HSP 1 Score: 122.5 bits (306), Expect = 2.2e-24
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + GFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFP +LETTA VHPG SGGAVVNS+GRM
Sbjct: 552 KSGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPAMLETTAAVHPGGSGGAVVNSDGRM 611

Query: 63  IGLVT 68
           IGLVT
Sbjct: 612 IGLVT 616

BLAST of Spg032308 vs. NCBI nr
Match: XP_038881508.1 (glyoxysomal processing protease, glyoxysomal isoform X1 [Benincasa hispida])

HSP 1 Score: 122.5 bits (306), Expect = 2.2e-24
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + GFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFP +LETTA VHPG SGGAVVNS+GRM
Sbjct: 564 KSGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPAMLETTAAVHPGGSGGAVVNSDGRM 623

Query: 63  IGLVT 68
           IGLVT
Sbjct: 624 IGLVT 628

BLAST of Spg032308 vs. NCBI nr
Match: XP_038881509.1 (glyoxysomal processing protease, glyoxysomal isoform X2 [Benincasa hispida])

HSP 1 Score: 122.5 bits (306), Expect = 2.2e-24
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + GFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFP +LETTA VHPG SGGAVVNS+GRM
Sbjct: 561 KSGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPAMLETTAAVHPGGSGGAVVNSDGRM 620

Query: 63  IGLVT 68
           IGLVT
Sbjct: 621 IGLVT 625

BLAST of Spg032308 vs. NCBI nr
Match: XP_038881511.1 (glyoxysomal processing protease, glyoxysomal isoform X4 [Benincasa hispida] >XP_038881512.1 glyoxysomal processing protease, glyoxysomal isoform X4 [Benincasa hispida])

HSP 1 Score: 122.5 bits (306), Expect = 2.2e-24
Identity = 58/65 (89.23%), Postives = 61/65 (93.85%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + GFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFP +LETTA VHPG SGGAVVNS+GRM
Sbjct: 448 KSGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPAMLETTAAVHPGGSGGAVVNSDGRM 507

Query: 63  IGLVT 68
           IGLVT
Sbjct: 508 IGLVT 512

BLAST of Spg032308 vs. ExPASy Swiss-Prot
Match: Q8VZD4 (Glyoxysomal processing protease, glyoxysomal OS=Arabidopsis thaliana OX=3702 GN=DEG15 PE=1 SV=2)

HSP 1 Score: 75.5 bits (184), Expect = 4.0e-13
Identity = 44/79 (55.70%), Postives = 53/79 (67.09%), Query Frame = 0

Query: 5   GFSPSVCSGVVANVVKAKIP-SSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRMI 64
           G SPS+CSGVVA VV AK   ++      +  FP +LETTA VHPG SGGAV+NS G MI
Sbjct: 533 GLSPSICSGVVAKVVHAKRRLNTQSISQEVAEFPAMLETTAAVHPGGSGGAVLNSSGHMI 592

Query: 65  GLVT---RYGT-TKLPVLS 79
           GLVT   R+G  T +P L+
Sbjct: 593 GLVTSNARHGAGTVIPHLN 611

BLAST of Spg032308 vs. ExPASy Swiss-Prot
Match: P39668 (Uncharacterized serine protease YyxA OS=Bacillus subtilis (strain 168) OX=224308 GN=yyxA PE=3 SV=2)

HSP 1 Score: 45.4 bits (106), Expect = 4.5e-04
Identity = 21/60 (35.00%), Postives = 39/60 (65.00%), Query Frame = 0

Query: 6   FSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRMIGL 65
           F+ SV  GV++   +A IP   +     ++   +L+T A ++PG+SGGA++N +G++IG+
Sbjct: 199 FAGSVTQGVISGTERA-IPVDSNGDGQPDWNAEVLQTDAAINPGNSGGALLNMDGKVIGI 257

BLAST of Spg032308 vs. ExPASy Swiss-Prot
Match: Q5HQE2 (Serine protease HtrA-like OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=SERP0611 PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.9e-04
Identity = 22/62 (35.48%), Postives = 37/62 (59.68%), Query Frame = 0

Query: 6   FSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRMIGL 65
           F  SV  G+V+  +   +P    + D+ +      +  A V+PG+SGGAVV+ +GR+IG+
Sbjct: 391 FKGSVSQGIVSG-LNRHVPVDIDKNDNYDALMKAFQIDAPVNPGNSGGAVVDRDGRLIGI 450

Query: 66  VT 68
           V+
Sbjct: 451 VS 451

BLAST of Spg032308 vs. ExPASy Swiss-Prot
Match: Q8CT52 (Serine protease HtrA-like OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=SE_0722/SE_0723 PE=3 SV=2)

HSP 1 Score: 45.1 bits (105), Expect = 5.9e-04
Identity = 22/62 (35.48%), Postives = 37/62 (59.68%), Query Frame = 0

Query: 6   FSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRMIGL 65
           F  SV  G+V+  +   +P    + D+ +      +  A V+PG+SGGAVV+ +GR+IG+
Sbjct: 391 FKGSVSQGIVSG-LNRHVPVDIDKNDNYDALMKAFQIDAPVNPGNSGGAVVDRDGRLIGI 450

Query: 66  VT 68
           V+
Sbjct: 451 VS 451

BLAST of Spg032308 vs. ExPASy Swiss-Prot
Match: Q2FI55 (Serine protease HtrA-like OS=Staphylococcus aureus (strain USA300) OX=367830 GN=SAUSA300_0923 PE=3 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 1.0e-03
Identity = 20/61 (32.79%), Postives = 36/61 (59.02%), Query Frame = 0

Query: 6   FSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRMIGL 65
           F  +V  G+++  +   +P  + + +  +      +  A V+PG+SGGAVVN EG++IG+
Sbjct: 575 FKGTVTEGIISG-LNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNSGGAVVNREGKLIGV 634

Query: 66  V 67
           V
Sbjct: 635 V 634

BLAST of Spg032308 vs. ExPASy TrEMBL
Match: A0A6J1CLH2 (glyoxysomal processing protease, glyoxysomal isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012364 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 4.1e-24
Identity = 57/65 (87.69%), Postives = 60/65 (92.31%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + GFSPSVCSGVVANVVKAKIPSS+HQGDSLEYFP ILETTA VHPG SGGAVVNSEG M
Sbjct: 450 KSGFSPSVCSGVVANVVKAKIPSSHHQGDSLEYFPAILETTAAVHPGGSGGAVVNSEGHM 509

Query: 63  IGLVT 68
           +GLVT
Sbjct: 510 VGLVT 514

BLAST of Spg032308 vs. ExPASy TrEMBL
Match: A0A6J1CK76 (glyoxysomal processing protease, glyoxysomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012364 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 4.1e-24
Identity = 57/65 (87.69%), Postives = 60/65 (92.31%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + GFSPSVCSGVVANVVKAKIPSS+HQGDSLEYFP ILETTA VHPG SGGAVVNSEG M
Sbjct: 566 KSGFSPSVCSGVVANVVKAKIPSSHHQGDSLEYFPAILETTAAVHPGGSGGAVVNSEGHM 625

Query: 63  IGLVT 68
           +GLVT
Sbjct: 626 VGLVT 630

BLAST of Spg032308 vs. ExPASy TrEMBL
Match: A0A6J1INF4 (glyoxysomal processing protease, glyoxysomal isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478559 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 9.0e-24
Identity = 57/65 (87.69%), Postives = 59/65 (90.77%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + GFSPSVCSGVVANVVKAKIP SYHQGDSLEYFP +LETTA VHPG SGGAVVNSEG M
Sbjct: 564 KSGFSPSVCSGVVANVVKAKIPPSYHQGDSLEYFPAMLETTAAVHPGCSGGAVVNSEGHM 623

Query: 63  IGLVT 68
           IGLVT
Sbjct: 624 IGLVT 628

BLAST of Spg032308 vs. ExPASy TrEMBL
Match: A0A6J1IUM2 (glyoxysomal processing protease, glyoxysomal isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111478559 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 9.0e-24
Identity = 57/65 (87.69%), Postives = 59/65 (90.77%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + GFSPSVCSGVVANVVKAKIP SYHQGDSLEYFP +LETTA VHPG SGGAVVNSEG M
Sbjct: 416 KSGFSPSVCSGVVANVVKAKIPPSYHQGDSLEYFPAMLETTAAVHPGCSGGAVVNSEGHM 475

Query: 63  IGLVT 68
           IGLVT
Sbjct: 476 IGLVT 480

BLAST of Spg032308 vs. ExPASy TrEMBL
Match: A0A6J1ILN8 (glyoxysomal processing protease, glyoxysomal isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478559 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 9.0e-24
Identity = 57/65 (87.69%), Postives = 59/65 (90.77%), Query Frame = 0

Query: 3   QDGFSPSVCSGVVANVVKAKIPSSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRM 62
           + GFSPSVCSGVVANVVKAKIP SYHQGDSLEYFP +LETTA VHPG SGGAVVNSEG M
Sbjct: 470 KSGFSPSVCSGVVANVVKAKIPPSYHQGDSLEYFPAMLETTAAVHPGCSGGAVVNSEGHM 529

Query: 63  IGLVT 68
           IGLVT
Sbjct: 530 IGLVT 534

BLAST of Spg032308 vs. TAIR 10
Match: AT1G28320.1 (protease-related )

HSP 1 Score: 75.5 bits (184), Expect = 2.9e-14
Identity = 44/79 (55.70%), Postives = 53/79 (67.09%), Query Frame = 0

Query: 5   GFSPSVCSGVVANVVKAKIP-SSYHQGDSLEYFPPILETTAVVHPGSSGGAVVNSEGRMI 64
           G SPS+CSGVVA VV AK   ++      +  FP +LETTA VHPG SGGAV+NS G MI
Sbjct: 533 GLSPSICSGVVAKVVHAKRRLNTQSISQEVAEFPAMLETTAAVHPGGSGGAVLNSSGHMI 592

Query: 65  GLVT---RYGT-TKLPVLS 79
           GLVT   R+G  T +P L+
Sbjct: 593 GLVTSNARHGAGTVIPHLN 611

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_031742825.11.1e-2873.40glyoxysomal processing protease, glyoxysomal isoform X6 [Cucumis sativus][more]
XP_038881510.12.2e-2489.23glyoxysomal processing protease, glyoxysomal isoform X3 [Benincasa hispida][more]
XP_038881508.12.2e-2489.23glyoxysomal processing protease, glyoxysomal isoform X1 [Benincasa hispida][more]
XP_038881509.12.2e-2489.23glyoxysomal processing protease, glyoxysomal isoform X2 [Benincasa hispida][more]
XP_038881511.12.2e-2489.23glyoxysomal processing protease, glyoxysomal isoform X4 [Benincasa hispida] >XP_... [more]
Match NameE-valueIdentityDescription
Q8VZD44.0e-1355.70Glyoxysomal processing protease, glyoxysomal OS=Arabidopsis thaliana OX=3702 GN=... [more]
P396684.5e-0435.00Uncharacterized serine protease YyxA OS=Bacillus subtilis (strain 168) OX=224308... [more]
Q5HQE25.9e-0435.48Serine protease HtrA-like OS=Staphylococcus epidermidis (strain ATCC 35984 / RP6... [more]
Q8CT525.9e-0435.48Serine protease HtrA-like OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA... [more]
Q2FI551.0e-0332.79Serine protease HtrA-like OS=Staphylococcus aureus (strain USA300) OX=367830 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1CLH24.1e-2487.69glyoxysomal processing protease, glyoxysomal isoform X2 OS=Momordica charantia O... [more]
A0A6J1CK764.1e-2487.69glyoxysomal processing protease, glyoxysomal isoform X1 OS=Momordica charantia O... [more]
A0A6J1INF49.0e-2487.69glyoxysomal processing protease, glyoxysomal isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A6J1IUM29.0e-2487.69glyoxysomal processing protease, glyoxysomal isoform X4 OS=Cucurbita maxima OX=3... [more]
A0A6J1ILN89.0e-2487.69glyoxysomal processing protease, glyoxysomal isoform X2 OS=Cucurbita maxima OX=3... [more]
Match NameE-valueIdentityDescription
AT1G28320.12.9e-1455.70protease-related [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF13365Trypsin_2coord: 8..65
e-value: 2.8E-9
score: 38.1
IPR043504Peptidase S1, PA clan, chymotrypsin-like foldGENE3D2.40.10.10coord: 2..82
e-value: 1.5E-10
score: 43.0
IPR039245Peroxisomal/glyoxysomal leader peptide-processing proteasePANTHERPTHR21004SERINE PROTEASE-RELATEDcoord: 5..67
IPR009003Peptidase S1, PA clanSUPERFAMILY50494Trypsin-like serine proteasescoord: 5..71

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg032308.1Spg032308.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016485 protein processing
cellular_component GO:0005777 peroxisome
molecular_function GO:0004252 serine-type endopeptidase activity