Spg032293 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg032293
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Resistance gene-like protein
Location	scaffold2: 33197612 .. 33198136 (-)
RNA-Seq Expression	Spg032293
Synteny	Spg032293

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAACAAAAATCTCCTTCTTCTTGTAATCGGCCCGAGATGAAGTATGATGTATTCATAAGTTTCAGAGGTAAAGATGTTCGTAAGAATTTTGTGGGATTTCTACATAGAGAATTAGAACGTTTGGGGCTAAAAGCTTTTCTGGATGAGAAGGAGTTTAAAATAGGGGACGATCTGTATGAGCTGTTCAAAATAATAGAAGAATCGAAATTTGTGATCGTCGTTCTTTCGAAAGACTATGCTTCTTCCAAGTGGTGTTTGAGAGAGCTGACCAAAATCATGGATTCTTCCATGGAAACAACACGAGCTGCTCGTGTTCTTCCTGTATTTTACCGTGTAGAACCTTCTGAAATTCGACATCCGTCTAACCCTTTTCTCAAGAAAAAGTTTGTTAAATTTAAAAGCGTTCTTAAAGGAGTTGATGATGGAGAAAAACAAAAGAAATTGGAGGAGATTAAACGTTGGAAGAAGGCCATGAAAGAGATTAGCACTCGAACTGGAGTTGTCATCACCAAAACCAGGTAA

mRNA sequence

ATGCAACAAAAATCTCCTTCTTCTTGTAATCGGCCCGAGATGAAGTATGATGTATTCATAAGTTTCAGAGGTAAAGATGTTCGTAAGAATTTTGTGGGATTTCTACATAGAGAATTAGAACGTTTGGGGCTAAAAGCTTTTCTGGATGAGAAGGAGTTTAAAATAGGGGACGATCTGTATGAGCTGTTCAAAATAATAGAAGAATCGAAATTTGTGATCGTCGTTCTTTCGAAAGACTATGCTTCTTCCAAGTGGTGTTTGAGAGAGCTGACCAAAATCATGGATTCTTCCATGGAAACAACACGAGCTGCTCGTGTTCTTCCTGTATTTTACCGTGTAGAACCTTCTGAAATTCGACATCCGTCTAACCCTTTTCTCAAGAAAAAGTTTGTTAAATTTAAAAGCGTTCTTAAAGGAGTTGATGATGGAGAAAAACAAAAGAAATTGGAGGAGATTAAACGTTGGAAGAAGGCCATGAAAGAGATTAGCACTCGAACTGGAGTTGTCATCACCAAAACCAGGTAA

Coding sequence (CDS)

ATGCAACAAAAATCTCCTTCTTCTTGTAATCGGCCCGAGATGAAGTATGATGTATTCATAAGTTTCAGAGGTAAAGATGTTCGTAAGAATTTTGTGGGATTTCTACATAGAGAATTAGAACGTTTGGGGCTAAAAGCTTTTCTGGATGAGAAGGAGTTTAAAATAGGGGACGATCTGTATGAGCTGTTCAAAATAATAGAAGAATCGAAATTTGTGATCGTCGTTCTTTCGAAAGACTATGCTTCTTCCAAGTGGTGTTTGAGAGAGCTGACCAAAATCATGGATTCTTCCATGGAAACAACACGAGCTGCTCGTGTTCTTCCTGTATTTTACCGTGTAGAACCTTCTGAAATTCGACATCCGTCTAACCCTTTTCTCAAGAAAAAGTTTGTTAAATTTAAAAGCGTTCTTAAAGGAGTTGATGATGGAGAAAAACAAAAGAAATTGGAGGAGATTAAACGTTGGAAGAAGGCCATGAAAGAGATTAGCACTCGAACTGGAGTTGTCATCACCAAAACCAGGTAA

Protein sequence

MQQKSPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLYELFKIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPFLKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITKTR

Homology

[Close]

BLAST of Spg032293 vs. NCBI nr
Match: XP_022961418.1 (toll/interleukin-1 receptor-like protein [Cucurbita moschata])

HSP 1 Score: 252.7 bits (644), Expect = 2.3e-63
Identity = 131/161 (81.37%), Postives = 150/161 (93.17%), Query Frame = 0


Query: 14  MKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLYELFKIIEESKFVI 73
           MKYDVFISFRGKDVRKNFVGFLH+ELERLGLKA +DEKEFKIG+DL++LFKIIEESKFVI
Sbjct: 1   MKYDVFISFRGKDVRKNFVGFLHQELERLGLKALVDEKEFKIGEDLFDLFKIIEESKFVI 60

Query: 74  VVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPFLKKKFVKF 133
           V+LS +YASSKWCLRELTKIM+ SME  + AR LPVFY+V+PSEIR+ SNPFLKKKFVKF
Sbjct: 61  VILSNNYASSKWCLRELTKIME-SMEMAQ-ARALPVFYQVQPSEIRNLSNPFLKKKFVKF 120

Query: 134 KSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITKTR 175
           KS +KGVDD + ++KLEEIKRWKKAMK+I+TRTGVVITK+R
Sbjct: 121 KSFMKGVDDQKMRRKLEEIKRWKKAMKKITTRTGVVITKSR 159

[Close]

BLAST of Spg032293 vs. NCBI nr
Match: KAG6590417.1 (TMV resistance protein N, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 245.0 bits (624), Expect = 4.9e-61
Identity = 131/161 (81.37%), Postives = 146/161 (90.68%), Query Frame = 0


Query: 14  MKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLYELFKIIEESKFVI 73
           MKYDVFISFRGKDVRKNFVGFLHRELERLGLKAF+DEKEFKI DDL++LFKIIEESKFVI
Sbjct: 1   MKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFVDEKEFKIRDDLFDLFKIIEESKFVI 60

Query: 74  VVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPFLKKKFVKF 133
           V+LS +YASSKWCLRELTKIM+  ME  + ARVLPVFYRV+PS+IR+ SNPFLKKKFVKF
Sbjct: 61  VILSNNYASSKWCLRELTKIME-CMEMAQ-ARVLPVFYRVQPSKIRNLSNPFLKKKFVKF 120

Query: 134 KSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITKTR 175
           KS +KGVDD + Q+KLEEIKRWKK    I+TRTGVVITK+R
Sbjct: 121 KSFMKGVDDQKMQRKLEEIKRWKK----ITTRTGVVITKSR 155

[Close]

BLAST of Spg032293 vs. NCBI nr
Match: XP_022961416.1 (toll/interleukin-1 receptor-like protein [Cucurbita moschata])

HSP 1 Score: 220.7 bits (561), Expect = 9.8e-54
Identity = 115/141 (81.56%), Postives = 129/141 (91.49%), Query Frame = 0


Query: 14  MKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLYELFKIIEESKFVI 73
           MKYDVFISFRGKDVRKNFVGFLH+ELERLGLKA +DEKEFKIGDDL++LFKIIEE KFVI
Sbjct: 1   MKYDVFISFRGKDVRKNFVGFLHQELERLGLKALVDEKEFKIGDDLFDLFKIIEELKFVI 60

Query: 74  VVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPFLKKKFVKF 133
           V+LS +YASSKWCLRELTKIM+ SME  + AR LPVFYRV+PSEIR+ SNPFLKKKFVKF
Sbjct: 61  VILSNNYASSKWCLRELTKIME-SMEMAQ-ARALPVFYRVQPSEIRNLSNPFLKKKFVKF 120

Query: 134 KSVLKGVDDGEKQKKLEEIKR 155
           KS +KGVDD + ++KLEEIKR
Sbjct: 121 KSFMKGVDDQKMRRKLEEIKR 139

[Close]

BLAST of Spg032293 vs. NCBI nr
Match: XP_011656060.2 (TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656061.2 TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656062.2 TMV resistance protein N isoform X1 [Cucumis sativus] >XP_031741444.1 TMV resistance protein N isoform X1 [Cucumis sativus] >KAE8648981.1 hypothetical protein Csa_009304 [Cucumis sativus])

HSP 1 Score: 192.2 bits (487), Expect = 3.8e-45
Identity = 101/172 (58.72%), Postives = 127/172 (73.84%), Query Frame = 0


Query: 1   MQQKSPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLY 60
           MQ  S SS +RP+M YDVFISFRG+DVR  F G+L+  L RLG+KAFLD K F IGDDL+
Sbjct: 1   MQSSSSSSLDRPKMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFLIGDDLH 60

Query: 61  ELFKIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRH 120
           +LFKII+ES+  IVVLS+DYAS+KWCLRELTKIMDS    T   RVLPVFY ++PS ++ 
Sbjct: 61  DLFKIIDESRSAIVVLSEDYASAKWCLRELTKIMDSM--GTSMERVLPVFYHIDPSIVKD 120

Query: 121 PSNPFLKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITK 173
            S  F         +VLK +D+ EK+K+L+E++ WK A+K+I   TGVVITK
Sbjct: 121 QSGTFKTSFDEHEANVLKEIDNQEKEKRLKELQNWKSALKKIGNHTGVVITK 170

[Close]

BLAST of Spg032293 vs. NCBI nr
Match: XP_031741445.1 (TMV resistance protein N isoform X2 [Cucumis sativus])

HSP 1 Score: 192.2 bits (487), Expect = 3.8e-45
Identity = 101/172 (58.72%), Postives = 127/172 (73.84%), Query Frame = 0


Query: 1   MQQKSPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLY 60
           MQ  S SS +RP+M YDVFISFRG+DVR  F G+L+  L RLG+KAFLD K F IGDDL+
Sbjct: 1   MQSSSSSSLDRPKMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFLIGDDLH 60

Query: 61  ELFKIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRH 120
           +LFKII+ES+  IVVLS+DYAS+KWCLRELTKIMDS    T   RVLPVFY ++PS ++ 
Sbjct: 61  DLFKIIDESRSAIVVLSEDYASAKWCLRELTKIMDSM--GTSMERVLPVFYHIDPSIVKD 120

Query: 121 PSNPFLKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITK 173
            S  F         +VLK +D+ EK+K+L+E++ WK A+K+I   TGVVITK
Sbjct: 121 QSGTFKTSFDEHEANVLKEIDNQEKEKRLKELQNWKSALKKIGNHTGVVITK 170

[Close]

BLAST of Spg032293 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 7.2e-23
Identity = 66/153 (43.14%), Postives = 94/153 (61.44%), Query Frame = 0


Query: 16  YDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDL-YELFKIIEESKFVIV 75
           YDVF+SFRG+D RK FVG L   L   G+  F+D+KE K G  +  EL K I ES+F +V
Sbjct: 12  YDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVV 71

Query: 76  VLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPFLKKKFVKFK 135
           V SK+YASS WCL EL KI++  +       V+PVFY V+PS +R  +  +    F KF+
Sbjct: 72  VFSKNYASSTWCLEELVKILE--IHEKFELIVVPVFYDVDPSTVRKQNGEY-AVCFTKFE 131

Query: 136 SVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTG 168
           + L  VDD +K      + RW++A+ +++  +G
Sbjct: 132 ANL--VDDRDK------VLRWREALTKVANISG 153

[Close]

BLAST of Spg032293 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 1.6e-22
Identity = 67/162 (41.36%), Postives = 95/162 (58.64%), Query Frame = 0


Query: 7   SSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDL-YELFKI 66
           SS +     YDVF+SFRG+D RK F   L+  L   G+K F D+K  + G  +  EL K 
Sbjct: 3   SSSSSSRWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKA 62

Query: 67  IEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPF 126
           IEES+F IVV S++YA+S+WCL EL KIM+   +T     V+P+FY V+PS +R+    F
Sbjct: 63  IEESQFAIVVFSENYATSRWCLNELVKIME--CKTRFKQTVIPIFYDVDPSHVRNQKESF 122

Query: 127 LKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTG 168
             K F + ++  K  DD      +E I+RW+ A+ E +   G
Sbjct: 123 -AKAFEEHETKYK--DD------VEGIQRWRIALNEAANLKG 153

[Close]

BLAST of Spg032293 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.3e-21
Identity = 67/173 (38.73%), Postives = 94/173 (54.34%), Query Frame = 0


Query: 1   MQQKSPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLY 60
           M+  SPSS      ++DVF+SFRG D R NF G L + L   G+ +F+D++  + GD+L 
Sbjct: 1   MESSSPSSA-----EFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFIDDR-LRRGDNLT 60

Query: 61  ELFKIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRH 120
            LF  IE+SK  I+V S +YA+S WCLREL KI++     +    V+P+FY+V+ S++  
Sbjct: 61  ALFDRIEKSKIAIIVFSTNYANSAWCLRELVKILE--CRNSNQQLVVPIFYKVDKSDVEK 120

Query: 121 PSNPFLKKKFVKFK---SVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVI 171
             N F     V FK       GV         EEI  WK A+   S   G V+
Sbjct: 121 QRNSFA----VPFKLPELTFPGVTP-------EEISSWKAALASASNILGYVV 154

[Close]

BLAST of Spg032293 vs. ExPASy Swiss-Prot
Match: Q9SSN3 (Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR PE=1 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.5e-20
Identity = 60/154 (38.96%), Postives = 92/154 (59.74%), Query Frame = 0


Query: 15  KYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLY-ELFKIIEESKFVI 74
           KYDVF+SFRG D R NF+ FL++EL R  ++ F D+KE + G     EL   IE S+F +
Sbjct: 8   KYDVFLSFRGHDTRHNFISFLYKELVRRSIRTFKDDKELENGQRFSPELKSPIEVSRFAV 67

Query: 75  VVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPFLKKKFVKF 134
           VV+S++YA+S WCL EL  IMD   E   +  V+P+FY VEP+ +R  +   L ++F K 
Sbjct: 68  VVVSENYAASSWCLDELVTIMD--FEKKGSITVMPIFYGVEPNHVRWQTG-VLAEQFKKH 127

Query: 135 KSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTG 168
            S          ++  E++ +W++A+   +  +G
Sbjct: 128 AS----------REDPEKVLKWRQALTNFAQLSG 148

[Close]

BLAST of Spg032293 vs. ExPASy Swiss-Prot
Match: Q40253 (Disease resistance protein L6 OS=Linum usitatissimum OX=4006 GN=L6 PE=1 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 5.7e-20
Identity = 58/162 (35.80%), Postives = 90/162 (55.56%), Query Frame = 0


Query: 12  PEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDL-YELFKIIEESK 71
           P ++Y+VF+SFRG D R+ F  FL++ L R  +  F D+ E   G ++   L + I++SK
Sbjct: 57  PSVEYEVFLSFRGPDTREQFTDFLYQSLRRYKIHTFRDDDELLKGKEIGPNLLRAIDQSK 116

Query: 72  FVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPFLKKKF 131
             + ++S  YA SKWCL EL +I+    E  R   +LP+FY V+PS++RH +  + KK F
Sbjct: 117 IYVPIISSGYADSKWCLMELAEIVRRQEEDPRRI-ILPIFYMVDPSDVRHQTGCY-KKAF 176

Query: 132 VKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITK 173
            K  +   G          + I+ WK A+K++    G  I K
Sbjct: 177 RKHANKFDG----------QTIQNWKDALKKVGDLKGWHIGK 206

[Close]

BLAST of Spg032293 vs. ExPASy TrEMBL
Match: A0A6J1HBR4 (toll/interleukin-1 receptor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111461999 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.1e-63
Identity = 131/161 (81.37%), Postives = 150/161 (93.17%), Query Frame = 0


Query: 14  MKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLYELFKIIEESKFVI 73
           MKYDVFISFRGKDVRKNFVGFLH+ELERLGLKA +DEKEFKIG+DL++LFKIIEESKFVI
Sbjct: 1   MKYDVFISFRGKDVRKNFVGFLHQELERLGLKALVDEKEFKIGEDLFDLFKIIEESKFVI 60

Query: 74  VVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPFLKKKFVKF 133
           V+LS +YASSKWCLRELTKIM+ SME  + AR LPVFY+V+PSEIR+ SNPFLKKKFVKF
Sbjct: 61  VILSNNYASSKWCLRELTKIME-SMEMAQ-ARALPVFYQVQPSEIRNLSNPFLKKKFVKF 120

Query: 134 KSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITKTR 175
           KS +KGVDD + ++KLEEIKRWKKAMK+I+TRTGVVITK+R
Sbjct: 121 KSFMKGVDDQKMRRKLEEIKRWKKAMKKITTRTGVVITKSR 159

[Close]

BLAST of Spg032293 vs. ExPASy TrEMBL
Match: A0A6J1HAB0 (toll/interleukin-1 receptor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111461997 PE=4 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.8e-54
Identity = 115/141 (81.56%), Postives = 129/141 (91.49%), Query Frame = 0


Query: 14  MKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLYELFKIIEESKFVI 73
           MKYDVFISFRGKDVRKNFVGFLH+ELERLGLKA +DEKEFKIGDDL++LFKIIEE KFVI
Sbjct: 1   MKYDVFISFRGKDVRKNFVGFLHQELERLGLKALVDEKEFKIGDDLFDLFKIIEELKFVI 60

Query: 74  VVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPFLKKKFVKF 133
           V+LS +YASSKWCLRELTKIM+ SME  + AR LPVFYRV+PSEIR+ SNPFLKKKFVKF
Sbjct: 61  VILSNNYASSKWCLRELTKIME-SMEMAQ-ARALPVFYRVQPSEIRNLSNPFLKKKFVKF 120

Query: 134 KSVLKGVDDGEKQKKLEEIKR 155
           KS +KGVDD + ++KLEEIKR
Sbjct: 121 KSFMKGVDDQKMRRKLEEIKR 139

[Close]

BLAST of Spg032293 vs. ExPASy TrEMBL
Match: A0A0A0KXU0 (TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647530 PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 1.8e-45
Identity = 101/172 (58.72%), Postives = 127/172 (73.84%), Query Frame = 0


Query: 1   MQQKSPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLY 60
           MQ  S SS +RP+M YDVFISFRG+DVR  F G+L+  L RLG+KAFLD K F IGDDL+
Sbjct: 68  MQSSSSSSLDRPKMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFLIGDDLH 127

Query: 61  ELFKIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRH 120
           +LFKII+ES+  IVVLS+DYAS+KWCLRELTKIMDS    T   RVLPVFY ++PS ++ 
Sbjct: 128 DLFKIIDESRSAIVVLSEDYASAKWCLRELTKIMDSM--GTSMERVLPVFYHIDPSIVKD 187

Query: 121 PSNPFLKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITK 173
            S  F         +VLK +D+ EK+K+L+E++ WK A+K+I   TGVVITK
Sbjct: 188 QSGTFKTSFDEHEANVLKEIDNQEKEKRLKELQNWKSALKKIGNHTGVVITK 237

[Close]

BLAST of Spg032293 vs. ExPASy TrEMBL
Match: M4QSV5 (Resistance gene-like protein OS=Cucumis melo OX=3656 GN=RGH11 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 2.4e-45
Identity = 101/174 (58.05%), Postives = 127/174 (72.99%), Query Frame = 0


Query: 1   MQQKSPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLY 60
           MQ  S SS +RP+M YDVFISFRG+DVR  F G+L+  L RLG+KAFLD K F IGDDL+
Sbjct: 1   MQSSSSSSLDRPKMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFLIGDDLH 60

Query: 61  ELFKIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRH 120
           +LFKII+ES+  IVVLS+DYAS+KWCLRELTKIMDS    T   RVLPVFY ++PS ++ 
Sbjct: 61  DLFKIIDESRSAIVVLSEDYASAKWCLRELTKIMDSM--GTSMERVLPVFYHIDPSIVKD 120

Query: 121 PSNPFLKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITKTR 175
            S  F         + LK +D+ EK+K+L+E++ WK A+K+I   TGVVITK R
Sbjct: 121 QSGTFKTSFDEHEANALKEIDNQEKEKRLKELQNWKNALKKIGNHTGVVITKNR 172

[Close]

BLAST of Spg032293 vs. ExPASy TrEMBL
Match: A0A1S4E362 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498648 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 5.3e-45
Identity = 100/172 (58.14%), Postives = 126/172 (73.26%), Query Frame = 0


Query: 1   MQQKSPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLY 60
           MQ  S SS +RP+M YDVFISFRG+DVR  F G+L+  L RLG+KAFLD K F IGDDL+
Sbjct: 1   MQSSSSSSLDRPKMNYDVFISFRGRDVRHTFAGYLYDALNRLGIKAFLDNKRFLIGDDLH 60

Query: 61  ELFKIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRH 120
           +LFKII+ES+  IVVLS+DYAS+KWCLRELTKIMDS    T   RVLPVFY ++PS ++ 
Sbjct: 61  DLFKIIDESRSAIVVLSEDYASAKWCLRELTKIMDSM--GTSMERVLPVFYHIDPSIVKD 120

Query: 121 PSNPFLKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTGVVITK 173
            S  F         + LK +D+ EK+K+L+E++ WK A+K+I   TGVVITK
Sbjct: 121 QSGTFKTSFDEHEANALKEIDNQEKEKRLKELQNWKNALKKIGNHTGVVITK 170

[Close]

BLAST of Spg032293 vs. TAIR 10
Match: AT1G72860.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 110.9 bits (276), Expect = 1.0e-24
Identity = 59/162 (36.42%), Postives = 96/162 (59.26%), Query Frame = 0


Query: 7   SSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDL-YELFKI 66
           SS + P  KYDVF+SFRG+D RKN V  LH++L   G+  F D+K+ ++GD +  E+ + 
Sbjct: 3   SSSSSPIWKYDVFLSFRGEDTRKNIVSHLHKQLVDKGVVTFKDDKKLELGDSISEEISRA 62

Query: 67  IEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNPF 126
           I+ S + +V+LS++YASS WCL EL  +MD  +      +V+P+FY V+PS +RH +  F
Sbjct: 63  IQNSTYALVILSENYASSSWCLDELRMVMD--LHLKNKIKVVPIFYGVDPSHVRHQTGSF 122

Query: 127 LKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTG 168
              K+             +  K   ++  W++A+ +I++  G
Sbjct: 123 TFDKY-------------QDSKMPNKVTTWREALTQIASLAG 149

[Close]

BLAST of Spg032293 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 110.2 bits (274), Expect = 1.7e-24
Identity = 69/172 (40.12%), Postives = 102/172 (59.30%), Query Frame = 0


Query: 5   SPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLY-ELF 64
           S SS +    K DVF+SFRG+DVRK FV  L  E +R+G+KAF D+ + + G  +  EL 
Sbjct: 7   SSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPELI 66

Query: 65  KIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSN 124
             I+ S+F IVV+S++YA+S WCL EL KIM+ + +T     ++P+FY V+PS++R    
Sbjct: 67  DAIKGSRFAIVVVSRNYAASSWCLDELLKIMECNKDT-----IVPIFYEVDPSDVRRQRG 126

Query: 125 PF--------LKKKFVKFKSVLK--GVDDGEKQKKLEEIKRWKKAMKEISTR 166
            F         K+K  K+K  LK      GE  +  ++ K  KK +K+IS +
Sbjct: 127 SFGEDVESHSDKEKVGKWKEALKKLAAISGEDSRNWDDSKLIKKIVKDISDK 173

[Close]

BLAST of Spg032293 vs. TAIR 10
Match: AT4G09430.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 107.1 bits (266), Expect = 1.5e-23
Identity = 67/168 (39.88%), Postives = 97/168 (57.74%), Query Frame = 0


Query: 1   MQQKSPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDLY 60
           M   S SS  R + +YDVF+SFRG D R N V +LH+ L  +G++ F D+KE + GD + 
Sbjct: 1   MASSSTSSPTRVK-EYDVFLSFRGADTRNNIVSYLHKALVDVGIRTFKDDKELEEGDIIS 60

Query: 61  E-LFKIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIR 120
           E L   I+ S F +VVLS+ Y +S WCL EL  IM+ S++      V+P+FY+VEPS++R
Sbjct: 61  EKLVNAIQTSWFAVVVLSEKYVTSSWCLEELRHIMELSIQDD--IIVVPIFYKVEPSDVR 120

Query: 121 HPSNPFLKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTG 168
           +  N F  K        L+   D EK      I +WK A+ ++   +G
Sbjct: 121 YQKNSFEVK--------LQHYRDPEK------ILKWKGALTQVGNMSG 151

[Close]

BLAST of Spg032293 vs. TAIR 10
Match: AT1G17615.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 105.9 bits (263), Expect = 3.3e-23
Identity = 58/164 (35.37%), Postives = 97/164 (59.15%), Query Frame = 0


Query: 5   SPSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDL-YELF 64
           S SS +    K+DVF+SFRGKD R+ F+ FL++EL  +G++ F D+ E + G  +  +L 
Sbjct: 4   SSSSSSATPQKFDVFLSFRGKDTRRTFISFLYKELIEMGIRTFKDDVELQSGRRIASDLL 63

Query: 65  KIIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSN 124
             IE SK  +V++SK+Y++S WCL+EL  IMD  +E   +  V+P+FY VEP+ +R    
Sbjct: 64  AAIENSKIAVVIISKNYSASPWCLQELVMIMD--VEKKGSIIVMPIFYNVEPAHVRR--- 123

Query: 125 PFLKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTG 168
                   + + V K     E ++  E +  W++A+  +++ +G
Sbjct: 124 --------QIEQVAKQFRKHEGRENYETVVSWRQALTNLASISG 154

[Close]

BLAST of Spg032293 vs. TAIR 10
Match: AT1G65850.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 104.8 bits (260), Expect = 7.3e-23
Identity = 65/163 (39.88%), Postives = 98/163 (60.12%), Query Frame = 0


Query: 6   PSSCNRPEMKYDVFISFRGKDVRKNFVGFLHRELERLGLKAFLDEKEFKIGDDL-YELFK 65
           PSS +  +  + VF SFRG+DVR+ F+  +H+E +R G+  F+D  E K G+ +  E+  
Sbjct: 23  PSSSSSHKWTHQVFPSFRGEDVRRGFLSHIHKEFQRKGITPFID-NEIKRGESIGLEIIH 82

Query: 66  IIEESKFVIVVLSKDYASSKWCLRELTKIMDSSMETTRAARVLPVFYRVEPSEIRHPSNP 125
            I ESK  IV+LS++YASS WCL EL +IM    E ++   V+P+FYRV+PS++      
Sbjct: 83  AIRESKIAIVLLSRNYASSSWCLDELVEIMKCKEEFSQI--VIPIFYRVDPSDV------ 142

Query: 126 FLKKKFVKFKSVLKGVDDGEKQKKLEEIKRWKKAMKEISTRTG 168
             KK    F +V K    G   K  E I++W++A+ ++ T TG
Sbjct: 143 --KKLTGNFGNVFKNNCVG---KTNEVIRKWRQALAKMGTTTG 171

The following BLAST results are available for this feature:

BLAST of Spg032293 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961418.1	2.3e-63	81.37	toll/interleukin-1 receptor-like protein [Cucurbita moschata]	[more]
KAG6590417.1	4.9e-61	81.37	TMV resistance protein N, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022961416.1	9.8e-54	81.56	toll/interleukin-1 receptor-like protein [Cucurbita moschata]	[more]
XP_011656060.2	3.8e-45	58.72	TMV resistance protein N isoform X1 [Cucumis sativus] >XP_011656061.2 TMV resist...	[more]
XP_031741445.1	3.8e-45	58.72	TMV resistance protein N isoform X2 [Cucumis sativus]	[more]

BLAST of Spg032293 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A290U7C4	7.2e-23	43.14	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
Q40392	1.6e-22	41.36	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]
Q9SZ66	2.3e-21	38.73	Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=...	[more]
Q9SSN3	1.5e-20	38.96	Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR ...	[more]
Q40253	5.7e-20	35.80	Disease resistance protein L6 OS=Linum usitatissimum OX=4006 GN=L6 PE=1 SV=1	[more]

BLAST of Spg032293 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBR4	1.1e-63	81.37	toll/interleukin-1 receptor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1HAB0	4.8e-54	81.56	toll/interleukin-1 receptor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A0A0KXU0	1.8e-45	58.72	TIR domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G647530 PE=4 SV...	[more]
M4QSV5	2.4e-45	58.05	Resistance gene-like protein OS=Cucumis melo OX=3656 GN=RGH11 PE=4 SV=1	[more]
A0A1S4E362	5.3e-45	58.14	TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498648 PE=4 SV=1	[more]

BLAST of Spg032293 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G72860.1	1.0e-24	36.42	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT5G17680.1	1.7e-24	40.12	disease resistance protein (TIR-NBS-LRR class), putative	[more]
AT4G09430.1	1.5e-23	39.88	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT1G17615.1	3.3e-23	35.37	Disease resistance protein (TIR-NBS class)	[more]
AT1G65850.1	7.3e-23	39.88	Disease resistance protein (TIR-NBS-LRR class) family	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 145..165
None	No IPR available	PANTHER	PTHR32009:SF84	TOLL/INTERLEUKIN-LIKE RECEPTOR-PROTEIN	coord: 13..172
None	No IPR available	PANTHER	PTHR32009	TMV RESISTANCE PROTEIN N-LIKE	coord: 13..172
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 15..165 e-value: 2.3E-35 score: 133.5
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF01582	TIR	coord: 15..163 e-value: 6.2E-30 score: 104.3
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROSITE	PS50104	TIR	coord: 14..174 score: 32.921486
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	GENE3D	3.40.50.10140		coord: 9..174 e-value: 5.9E-48 score: 164.8
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	SUPERFAMILY	52200	Toll/Interleukin receptor TIR domain	coord: 5..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg032293.1	Spg032293.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
molecular_function	GO:0003953	NAD+ nucleosidase activity