Spg032223 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg032223
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRNase H domain-containing protein
Locationscaffold2: 33236787 .. 33245153 (+)
RNA-Seq ExpressionSpg032223
SyntenySpg032223
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCACCTCGGAGAGCCAGGAGAATCAGGAAATATGGACTCCCCCACCGGTCGGAATTTTGAAGATCAACGTGGATGCATCGTGGAATGATGATCAGAGACGAGGTGGGATCGGATGGATAATTCGTGATTCTCAAGGATCTCCTGTAGGAATGGGAAGCGCAAGGATCAGGAAAAGATGGTCAATTTGCTCCCTGGAAGCAACAACTATCAACGAGAGTTTAGAAGCTTACCTGGCTTCAACGAGAGAATCTCGGTCTCAAATCATCCTCGAATCAGAATCGATCGAGGTGGTTAGGGCTCTAAATCATGAGATCGCAAATGAATCGGAGACGCGAGTGATTTTAGAAGAAATCGACTTGAAGGCGTCTCTCATTGGTGAAGTTTCGTTCGTGAAATGTCCCCGAACGGCTAACGGAATCGCGCACTCCCTCGCGCGTTCAGCGGCTGGATTTCAGCCGAATTTTTCGCCGTCTTTCGTTGTTGAACATGGTAGTTTTGGGCAGTTTTTTTCTTCCATGTTGGAAGACAAATGGGTTTGGAGGGAAGGGAATTTCCCGTTTTTTGTATTCCAATTATTGTGGAAGGATATTAGTGTACCCACTTTCTCAGGTTTTTAATGCTATTTATTTATTTCTCAAAAAAAAAGAAAGAGAGAAAGTAAGTTGAGAATGAAAGAGCATTTGGAACATGACCTGGTAAATAAAAGATTTGTTATAAGATTGGAAAATTTCAACAGTAGAAAAAACAAATAATCAACTGCTTCTACATATATGATCAAAGCTCCATTTCGCACACCTTTCAATTTTGAGAGATGTTGGTAAAATAACTAAAAGGTATAATTTATGTTTAAGTTATGAAAATTTAAGAAAAGAAAAATCTAGGATTTTTTTTTTAAAACAGAAGATAGAAAAAAATGCAAGGAAAAAAAAAACTACAAAATCGTTTGAAGGAAGTATTGAGAGCAAAGATGGAACAAAATTGACGCAATCGGTTACGGAATTTGATTGGTCATATTTAAAAGGTTAAATTACAAGTTTAGTCATTGAACTTTTAGGGTTGTGTTAATTTGATCCCTCAACTTTAAAAAGTGTCTAGCAGGTCCCTCAACTTTCAATCTTGTGTTTAATAGGTCCTTGAACTTTAAAAATGTCTAATAAGTCTTCGAACTTTTAATTTTGTATCTAATAGATCCCTCACATATTTGATTTTTTTTTTTAAAAAAAAAATTATCCAATCTACTAGATATAAGATTGAATTTTATACCTATTGGGACCCTAAACATCCAATTTTATATCTAGTAGGCTTGTCAATTTTAAAAAACATTTAATAGGTCAAGAACTTATGAGACATAAAATTGAAAGTTCTGGACCTATTGGACACAAAAGTGAAAGTGTAAGAACCTACTTAATGTTTATTAAAGTTCAGGGACCTATTAGATACAAAATTGAAAGTTTGAGGACCTAGTCGAAACTTTTTAAAGTTTATTGGGACTAAATTGACACAACTCTGAAAGCTTAAGGACTAAACTTGTAATTAAACCTATCAAAGTCTATCAATGATAGTCACTGATAGATTTCGATTAACCGGAGTCTATCAGTGATAGACACTGATAGACTCTGAATAATCGGAGTCTATCAGTAGACTGATAGATTATGATTAATCACAGTCTATCGTTGATAAACTCTGATAATCGAAGTCTATCAGATAGATTCTAATTAACTAGAGTCTATAAGTGTCTATCAGTAATATACTCTGATAGATTTTGCTATATTTACAATTTTTTTTTATATTTTGCTATATTTGCAATTATATAATTCTTATTTGCTACCCAGTGCAAATTCTAAAAAAAAATTTGTATTTCTGAACATGAAAAGGAAGAAAAAAAAAAAAAAGAAATTGTATATTGGGCTTGGGCCTTCAAAAAGCATGCTATAAAAGGCATGAAAACAACCAAAAAATTGAACACTTTTGGGGTTGAAGACAGGGAGAGAGAGAGAGTCGCAGAGCCGAATTTCACAGAGAAAACTGGAGAGTGGGGCAATTTAACTTCCCTCTTCCCCCCAAAATTTTTCACTTTTCCCGCGGCACTTTCCCTCTCCTTTCTCTCTCTCTCTCTCTCTCTCTCTTCATTCTCTTTCGCTGCCGCTGCCCAATCCAATTTTCAACTCTCGGTAATTCCCTCTCTTCTTCTCTACTTCGCTTTCTTCTTGCTTCGAGGCTTAATTCTAGGGTTTTCTTCTCATTTCTCGGTAAATCCCTTTTCTTCTCTTGTTTCTGTCTTCTTTCTTCATTTTCGGCTTCAGTTTTTGCTTTTCTTTACTTAATTTTTTCATGCCTTAGCTTATGTCTGTGTTCGTTCTGCTCGTTGTTGTTCCGAATTGGTATTTGGTTGCCTATTTGCAAGGTTTTTCGTGTTTGATGCTGATTTCTTGAATTAGTTGTGCTATTGACTTGTTTTCGTCACTGTTTTCGTGTATGAAGTTGAACTTTTGATGGTGTTTTATTTACTGTTGAAGTATTGTGTTGTTTTGGTAGATGGGGGATGTTAGAGTAACGCAGTTGAGAATTAAAATGTTTTCTTAGATCGGTCTTTCTTTCTTTCTATCTCACGTTACCATAAATTGGTGGCTCTGTTTTCTCCTGTAAAACGGGCGGTGGGTGGCAATGGAGTTTATGATCACTGAAGTTGCTATGGTTTTATGGGAACCAAATCTAGGATCTTATAAAGTTATAATATGTGTTCATTGCGAATAACTTTGTCTCTCTCCCTCTAACTCACACAGAAAGATTTGTTGGCTTGGTATCTAGGAATTGCCAATGTTGAGACTACTTTTTTTCATTTCTTTTTTCTCTTTTAGTTCATACTAGAAAAAGCTAGCAAGTACATCAATAGTAAGATGGTTATCATCTTGCAAAATGTCTAGTTTCAAGATTTTCTTTACAGTCAATAAAGGAAGTCGGTAGTTTTGAGGCTAGTAAATGCCATGAAAAAAAATATTAAAGAGAAGCCGTAATAAAATTATAGCTACTAAAAGGAAGAAAAAAGATGTTTTGAAGATCATTAATAGCAAAATTTTCTTTATGGTCAAACCATCTTTACCAGCTCAAATCCAAATCAACCTTGCATCATTAAAAAAAATGAATATCATTAATACTAATTCTTTCCGCAAGCTGATGGAAGGTTGATTTGGATTTGAGCTGGTAAAAATGGTTTGAAACTTAAACAGTTGGTGGGTTTTTTTTTTTATGAAGAGTTTTACTAGTTTCTTAGTTGAGGTGAAGTGGCGAGAGTTACAGAGAAATTTGTATTCATTGGTTTTAGAATCCCTAAAAAGCATGTGCTTAATTGAACCTTGCAGCTAGGTGCAACGTGCTTAGACAGTGGGAAACTAGGGTTTGAATTAAAATCAAAGGAAATCTTAAACAAAAAGTAATTGGTTTGTTTCCTCGACTCATTCCCTTTGGTGAATGTATTTGAAAATGAAAAGACTAATGATTGGGGGAAATGGGAATGAAGTCCGATTTGGCAATGATAGTTTGAAATTTTAGGTATTTGAAATAACTTTATGAACTCTTTGTCATTGAAGTTGTGTTTTTCAAGGCACAAGGCATGTTGAAGTGCAACATCCCTTTGGGGCTTAAGTATGAAGTTAAAAGAAAAGCAAGGGTTTCTTTTTGTTTGAGTGTGCAACAGAAAAATACAAATAAATTTTTAGGTAAAATTCACTTTTGGTCCCTAGGATTTGAGCTTAGTTTCTATTTGGTCAAAGTTTAGTAATTAATACTTCTGGTCCTTGAGATATTTATTTAATTTCTATTTAATCCTTGAAGTTTCACTTTGGTCTCCTAATTTTGATTTTGATTTTTAATTAATTCTTGAAGAGTGAAATTGGTTATTAGTCCTCGAGTACAAAAAATAAGAAATAAAAAAAATTCAAATTCATTTTAAAAAATACATATTAACAAAGTCTAGTCAATATATGTTAACTATCACATCATGCCATCATTCTATTAGGTCAACTCCACTCTAGCACCATTTAGATACCGATTTCAAATTTTGGGAATCAATGAGAAATCAAATTCAAACTTTTGGGACCAAACATGTTAGTCTTAAAACCTCAAAGATGAAATAGAAACTAAATTCAGATCTCAACGACCAAAAAGGTTAGTTTTGAAAACCACAACCAATGAAAAACTAAGTTCAAATAACAAGGATCAAAATTGTATTTTTATCCAAAAAAAATCCTTTATTTTCTCACTTATGCTTCATCTTACTTGGGTATTCACTTTTTTTTGTGGCTCATTGTCCTCAAAGGGCCATTAGGCTTTCTTCTTTAAAACATTGATGGTAATTTATGGATCCACTATTTTCTTCTTAGTTATAGCTAGAACATCCTTCACTGAATTCTCTTTAATTCTGAATGTGGTAATATTGTGTATACCAGTTAGCTAACTAATTTTTCCCTTTGCATTGAATAGGAGGTTTTGGTCATTAGAACTTGGGAGATTTGAGCTCTTTATTCAAGGTTGTAATTTGCAGTAAGAAGTAAAGAATTGAGAATGTCGTTATGAATGATGATTGTAGAGAGTTGAGTCTGGAATATGTAGGGGCTGTGTACTTTGGTGTGGGCTGTATGTTTGGATTGTTCTCACCAGTGATAATAGTTTTGTAAATAAAAAAGTAATTTTCTAATGAAATGCATTCTTCATAAGGAATGAGATTCAGTTTTAGAAACTTGTGCCTATGGTCTGTCGAGGAAGCATCTTAATCCTAGCCAATTAGGTATTGACTGTGTGAATAACCTACGATCATAATGAATAAGTAGAGGACAAATATGAAGAAGATAATGCTTGTGATTGTTTCGGAGTTCCAAATTTCATTTTTGCTTGTATTTCTACTTTTTGCTCGTTTATTTCCTACCTACGGGCTCTGATTTTGTATATTTGAACTGTACTTGCAGATATTGATTTCAAGTATTAGAATTTTTGCACTTGTGGATGTTGTAGCTCCGAAACCTTTGATTAGATGCCAGTACGTCATTCATGCTCAACTATCAACTTTGGGTGCTGATGGACTGTCAACAGCACGAGTAGAGAAATCCGTTCTTCCAGTGTCTTCTGTGTTTGAAAATTTAAATGGTTAGTACTTGAATGTGGTAGTAATGGAGTGCAACAAGGAAGAGGCCATCAAGGCTATGAAAATTGCTGAGAAGAAGCTGGAAATCAGTGATTTTATTGGGGCAAGGAAGATGGCTCAGACAGCACATAGACTATTCCCTACACTTGAAAACATTACCCAACTGCTAACAGTATGTGAGATTCACTGTTCAGCACAGAATAGAATATATGGATCTGAAAATGACTGGTATGGGATTCTTCAGATTGAACAATCAGCTGATGAAACAACTATTAAAAAGCAATACAGGAAGCTCGCGCTGTTACTTCACCCTGATAAGAACAAGTTTGCTGGTGCAGAAGCTGCCTTTAAACTTATTGGTGAAGCCAACAGGCTTCTCTCTGATCAATCAAAGCGCAAGTTATATGACATGAAGTGTGGAGTTACAAGAAGAAATATTGCACCTTCCAAGCCCTCTCATGATCAGTCAAATGGATATGCAACTATGAACAAGCAAGAGAGTGGAACTGCAAATGGTTTCTCAAGTGTTCCTTTCTCACAGTTCCCTGGAAGGAATTCTTATAAACCACCACAATCACAGCAGGCTTTTTGGACTTGCTGCCCATATTGTAATGTTAGGTATCAATACCTGAAATGCTATCTTAGTAAGATGTTGCGTTGCCAGAATTGTGGCAGAGGTTTTCTCTCTTATGATTTGAATAGCCAAACTATGCCTCCAACATCCCACCAAGGTCACATCCCTCAGAAGAAAGTAGCTCCTGAGTCGGGACCCTCAAAATCTGCTGCACAAAACAAACAGAGTTCTGATAAGAGATCTCAGGATAGGTCTGCTGGAGTTGATCGAGCATCAAGGGAAAATTACACTGCTGGGAATGAGCCCAAATCAGATGCAGACATGACTGGGAAAGTAAAGTCAAGGTCTTATACAGCCACAAACACGGAAAAAAGAGCTGCCAAGTGTGAAAGTAGGAAGAGACCACGAAAGTCAGCTCAGACACCTTACAACAACTCAGAAAATGCCGAAGAGGTTGAAGATGCCGATGTATCTGGAAAGGATCCTGACCTGAGTAGAGGCAATTGTCAAAGGAGATCTTCAAGGAACAAACAACATGTTTCATACAAGAAATATTTAAGTGATGATGATGATAATTTACAGAGTCCAAAGAAGTCAAGGGGCAGTGGATCATCTATGGACTTGAAAGAGAAGATGAAAGATGTAACTGCAGATGTTGCAGCATCTAAAGTAGACAAATGTTCAGATGGTCATGGTACTGTTGATGGTCATAACAAGGGCATCAAACGTGAAGTACCTTCCCCCCATCCGGAAGTCTTGCCCAACATGAAACCCAAAGGTGAGGAACTGAAGGTAATGAGAGAAGAAAAAAACATGTCAGATCAAAATGATAAAAGATCCAAAGTTGAGGATTCGGATACAGAGAAAGACTGTGTGAAAGGAGTACATGTTTTGGTATGTGCTGATCCTGAGTTTAGCGATTTTGATAAGGGTAAAATGGAAGATTGTTTTGCGGTCAATCAGGTATGGGCTATATATGATACCATTGATGGCATGCCAAGATTTTATGCCCGCATTAGAAAAGTGTTCTCCCCTACATTCAAGCTCCAGATTACTTGGTTTGAACCAAATCCTGATGACAAAGGTGAAATTGATTGGAGCGATGCAGAATTGCCGATTGCATGTGGCAAGTATACACTAGGAGAGACTGAGGAAACTGTTGATCTTCCCATGTTCTCTCATTTGGTACATTGCCCCAAACAGGGAGTGAGGAATGCTTATTTGATGTATCCTAGAAAAGGAGAAACTTGGGCTCTTTTCAAGGATTGGGATATCAGATGGAGCTCAAACCCTGAAAAGCATGCAACTTTTGAATATGAATTCGTTGAGATCATGTCAGATTATGTTGCGGGTGCTGGCATCTCGGTTGCTTACATGGACAAGGTCAAAGGTTTTGTCTGCTTGTTTCAGACTACTGAGAAGCACAAAGTAAACTGTTTTAAGATTCCACCTAATGAGTTGTACAGATTTTCTCATCAAATTCCTTCAGTTAGAATGACTGGAGATGAAAGAGAAGGTGTTCCGAAAGGATCATTTGAATTAGATCCTGCTGCGCTTCCCCCCAATATTAATGACCATGTCGATCGTAATGACATCAAGGGGGGGACTGATGATGAAGCTGCTGCTGCTACTTCTGGAAGAATTGATCCATCTCATGATTGTAAATCCCCAAAATTGAAAGTGGAGGTGATTGGTCTAGAGAATAATCAAGCTGCAAACATACAGAAGAAGAGTAACTCAAAGAAGAGTAATCTGAAAAGTGAGGCCCCAACTATTGTACGAAGGTCTCCAAGAAAGTTGGACACGACTGGAAACGACACTCAGGTAAACGTGGACGAGTACATGCCTGAAGACAATGGAAGTAGAGTTGCAAGACAAAATGGCCTATCAACGGACAAGGAAAGCAGTGTGATTCATATAAATGGAGGAACGACCACTCCAAAGAAACATGGCAAGAATGGCAATGAGAGAGAAATAATCATACTTAGAAGATCACCGAGAGATTTGGCCAAGAAAAATGCAGGTTAATGTATGAAAGAGATGGAGAGCAGGCAATCTGATTCTTCCGAAGTTGAAAACAAATTTTGCTTTTGAGAATGGGAAGTATTCATCTCCATGTAAAATGAGCACAGTCCCAGTTGATTTCCTTTCTTTCTTTTCTTATTTTCTCATTCTAGAGAACTCTCGTGCACTGTTAAGTGCTCTTTTTCAGGAAGGCATCTATCGACGAGTTTGAGTTTCGAAGTTTCTAGCCGGAGAAAACTTCGGTTTTGCTCAGACCTCTAACAGTGAAAGGTTGAAGAAAGAGTTGGGTAATGCCATCAAGCAAATGGGAAGAGCAAGGAGTTGACTCAGGCTGTTGGGAGCTTAATATTTTGGTTCTAATTTGAAGATTGGTCTGCAAAAGCAGGAATTGTAAATTTGATTTTCCAACAGTTTAGTGTAGGAGTAGTCATTAGAGGGAGGGCCTCATCTGCTATGTTGACACTTTTGAAAGCATTATCATGTTTTATTGGATTTTGGATGAAGATGTTCATATATGGAGAGGGAGGCCTTTTGATATGTAACCACTAAATTCTTCTTGAGTTCTGATCATCCCATGTTTATCCATGTCTGTTCTACTATCTTGTTCTCATGTGCTATTTGATAATG

mRNA sequence

ATGCTCACCTCGGAGAGCCAGGAGAATCAGGAAATATGGACTCCCCCACCGGTCGGAATTTTGAAGATCAACGTGGATGCATCGTGGAATGATGATCAGAGACGAGGTGGGATCGGATGGATAATTCGTGATTCTCAAGGATCTCCTGTAGGAATGGGAAGCGCAAGGATCAGGAAAAGATGGTCAATTTGCTCCCTGGAAGCAACAACTATCAACGAGAGTTTAGAAGCTTACCTGGCTTCAACGAGAGAATCTCGGTCTCAAATCATCCTCGAATCAGAATCGATCGAGGTGGTTAGGGCTCTAAATCATGAGATCGCAAATGAATCGGAGACGCGAGTGATTTTAGAAGAAATCGACTTGAAGGCGTCTCTCATTGGTGAAGTTTCGTTCGTGAAATGTCCCCGAACGGCTAACGGAATCGCGCACTCCCTCGCGCGTTCAGCGGCTGGATTTCAGCCGAATTTTTCGCCGTCTTTCGTTGTTGAACATGCTTATGTCTGTGTTCGTTCTGCTCGTTGTTGTTCCGAATTGATATTGATTTCAAGTATTAGAATTTTTGCACTTGTGGATGTTGTAGCTCCGAAACCTTTGATTAGATGCCAGTACGTCATTCATGCTCAACTATCAACTTTGGGTGCTGATGGACTGTCAACAGCACGAGTAGAGAAATCCGTTCTTCCAGTGTCTTCTGTGTTTGAAAATTTAAATGGTTAG

Coding sequence (CDS)

ATGCTCACCTCGGAGAGCCAGGAGAATCAGGAAATATGGACTCCCCCACCGGTCGGAATTTTGAAGATCAACGTGGATGCATCGTGGAATGATGATCAGAGACGAGGTGGGATCGGATGGATAATTCGTGATTCTCAAGGATCTCCTGTAGGAATGGGAAGCGCAAGGATCAGGAAAAGATGGTCAATTTGCTCCCTGGAAGCAACAACTATCAACGAGAGTTTAGAAGCTTACCTGGCTTCAACGAGAGAATCTCGGTCTCAAATCATCCTCGAATCAGAATCGATCGAGGTGGTTAGGGCTCTAAATCATGAGATCGCAAATGAATCGGAGACGCGAGTGATTTTAGAAGAAATCGACTTGAAGGCGTCTCTCATTGGTGAAGTTTCGTTCGTGAAATGTCCCCGAACGGCTAACGGAATCGCGCACTCCCTCGCGCGTTCAGCGGCTGGATTTCAGCCGAATTTTTCGCCGTCTTTCGTTGTTGAACATGCTTATGTCTGTGTTCGTTCTGCTCGTTGTTGTTCCGAATTGATATTGATTTCAAGTATTAGAATTTTTGCACTTGTGGATGTTGTAGCTCCGAAACCTTTGATTAGATGCCAGTACGTCATTCATGCTCAACTATCAACTTTGGGTGCTGATGGACTGTCAACAGCACGAGTAGAGAAATCCGTTCTTCCAGTGTCTTCTGTGTTTGAAAATTTAAATGGTTAG

Protein sequence

MLTSESQENQEIWTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEATTINESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEVSFVKCPRTANGIAHSLARSAAGFQPNFSPSFVVEHAYVCVRSARCCSELILISSIRIFALVDVVAPKPLIRCQYVIHAQLSTLGADGLSTARVEKSVLPVSSVFENLNG
Homology
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BLAST of Spg032223 vs. NCBI nr
Match: XP_015384077.1 (uncharacterized protein LOC107176301 [Citrus sinensis] >XP_024039330.1 uncharacterized protein LOC112097966 [Citrus clementina])

HSP 1 Score: 83.6 bits (205), Expect = 2.6e-12
Identity = 54/161 (33.54%), Postives = 87/161 (54.04%), Query Frame = 0 

Query: 4   SESQENQEIWTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSI 63
           + S+  Q  W PPP  + K+NVDA+ N  ++  G+G +IRDS+ + V  G  +   + S+
Sbjct: 211 TSSKVPQHKWKPPPKNVFKVNVDAAINSKRQMAGLGAVIRDSENNFVATGIMQTGMKESV 270

Query: 64  CSLEATTINESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKA 123
              EA  I+  L+    + R + S +I+ES+ +EVV  +N+  +N++    I+EEI  + 
Sbjct: 271 SYAEAEAIDWGLK---LARRAALSTLIIESDCLEVVELVNNTKSNKTGLWWIIEEIQNQK 330

Query: 124 SLIGEVSFVKCPRTANGIAHSLARSAAGFQPNFSPSFVVEH 165
                V     PRT N  AHSLA+ A G     +P+  V+H
Sbjct: 331 RTFCNVIVNHIPRTCNVCAHSLAKFAVGAN---TPAVWVDH 365

[Close]
BLAST of Spg032223 vs. NCBI nr
Match: PNT77801.1 (hypothetical protein BRADI_1g69052v3, partial [Brachypodium distachyon])

HSP 1 Score: 83.2 bits (204), Expect = 3.4e-12
Identity = 50/149 (33.56%), Postives = 75/149 (50.34%), Query Frame = 0 

Query: 10  QEIWTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEAT 69
           +E+W  PP   +KINVDAS++ D+  G IG +IRD+ G  +   + ++     + S E T
Sbjct: 274 EEMWMKPPADFVKINVDASFSMDELSGTIGAVIRDTSGEFIVAANGKLPLVTDVVSAEVT 333

Query: 70  TINESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEV 129
            +   LE  +A   +   +II+  +++EVV ALN      S    I EE       +G+ 
Sbjct: 334 AVQLGLELAIA---QGCQRIIINGDNLEVVEALNRGERVYSSATAIFEECFALLKEVGKA 393

Query: 130 SFVKCPRTANGIAHSLARSAAGFQPNFSP 159
             + C R  N +AH LAR     Q  FSP
Sbjct: 394 QIIHCSRNTNSVAHELAR-----QAKFSP 414

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BLAST of Spg032223 vs. NCBI nr
Match: EOA22050.1 (hypothetical protein CARUB_v10002590mg, partial [Capsella rubella])

HSP 1 Score: 79.0 bits (193), Expect = 6.3e-11
Identity = 45/150 (30.00%), Postives = 80/150 (53.33%), Query Frame = 0 

Query: 9   NQEIWTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEA 68
           N+E W PPP   +K N D +WN +  + G+GW++R+  G  + MG+  +R+  S   +E 
Sbjct: 88  NEEKWRPPPRCWVKCNTDVAWNGEDTQSGMGWVLRNCSGEVLWMGAQALRRTRSALEVEL 147

Query: 69  TTINESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGE 128
             I  ++ + L   R    +II ES+S+ +V  LN++  N      ++++I     L+ +
Sbjct: 148 EAIRWAVTSML---RLDFQKIIFESDSLVLVNLLNND-ENWPAFAPLIQDIKDSLPLLED 207

Query: 129 VSFVKCPRTANGIAHSLARSAAGFQPNFSP 159
              V  PR  NG+A  +A+ +  F+ N+ P
Sbjct: 208 FKIVYTPRGCNGVADRVAKESLSFE-NYDP 232

[Close]
BLAST of Spg032223 vs. NCBI nr
Match: XP_006471930.1 (uncharacterized protein LOC102617744 [Citrus sinensis])

HSP 1 Score: 78.6 bits (192), Expect = 8.3e-11
Identity = 45/148 (30.41%), Postives = 79/148 (53.38%), Query Frame = 0 

Query: 2   LTSESQENQEIWTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRW 61
           +++  +E+Q+ W+PPP  +LK+NVDA+ N   ++ G+G ++RDS G  V  G  +   R 
Sbjct: 125 ISNTREESQQRWSPPPTNVLKLNVDAATNSKDQKVGLGAVLRDSNGRVVAAGIKQASFRK 184

Query: 62  SICSLEATTINESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDL 121
            +   EA  I   L+    + + + + +I+E++  +V   +N+   + SE   I+ EI  
Sbjct: 185 DVSFAEAEAIQWGLQ---IAKKAAAASLIVETDCKDVAELVNNTKGSRSEIFWIISEIQT 244

Query: 122 KASLIGEVSFVKCPRTANGIAHSLARSA 150
           +      + F   PR  N  AHSLA+ A
Sbjct: 245 QKREFQRIHFNFVPRFCNAHAHSLAKHA 269

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BLAST of Spg032223 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 78.2 bits (191), Expect = 1.1e-10
Identity = 50/137 (36.50%), Postives = 69/137 (50.36%), Query Frame = 0 

Query: 13  WTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEATTIN 72
           W PP     K+N +A+W  D   GGIGWI+RD +G  +      IR   +I  LE   I 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 73  ESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEVSFV 132
           E L A     +E    I LES+S+E +  L+ +  +++E   +LEEI      +  VS  
Sbjct: 139 EGLRAI---RQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMR 198

Query: 133 KCPRTANGIAHSLARSA 150
              R AN +AH LAR A
Sbjct: 199 HISREANKVAHGLARRA 212

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BLAST of Spg032223 vs. ExPASy TrEMBL
Match: A0A2K2DU38 (RNase H domain-containing protein (Fragment) OS=Brachypodium distachyon OX=15368 GN=BRADI_1g69052v3 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 1.6e-12
Identity = 50/149 (33.56%), Postives = 75/149 (50.34%), Query Frame = 0 

Query: 10  QEIWTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEAT 69
           +E+W  PP   +KINVDAS++ D+  G IG +IRD+ G  +   + ++     + S E T
Sbjct: 274 EEMWMKPPADFVKINVDASFSMDELSGTIGAVIRDTSGEFIVAANGKLPLVTDVVSAEVT 333

Query: 70  TINESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEV 129
            +   LE  +A   +   +II+  +++EVV ALN      S    I EE       +G+ 
Sbjct: 334 AVQLGLELAIA---QGCQRIIINGDNLEVVEALNRGERVYSSATAIFEECFALLKEVGKA 393

Query: 130 SFVKCPRTANGIAHSLARSAAGFQPNFSP 159
             + C R  N +AH LAR     Q  FSP
Sbjct: 394 QIIHCSRNTNSVAHELAR-----QAKFSP 414

[Close]
BLAST of Spg032223 vs. ExPASy TrEMBL
Match: R0HAP3 (RNase H domain-containing protein (Fragment) OS=Capsella rubella OX=81985 GN=CARUB_v10002590mg PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 3.1e-11
Identity = 45/150 (30.00%), Postives = 80/150 (53.33%), Query Frame = 0 

Query: 9   NQEIWTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEA 68
           N+E W PPP   +K N D +WN +  + G+GW++R+  G  + MG+  +R+  S   +E 
Sbjct: 88  NEEKWRPPPRCWVKCNTDVAWNGEDTQSGMGWVLRNCSGEVLWMGAQALRRTRSALEVEL 147

Query: 69  TTINESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGE 128
             I  ++ + L   R    +II ES+S+ +V  LN++  N      ++++I     L+ +
Sbjct: 148 EAIRWAVTSML---RLDFQKIIFESDSLVLVNLLNND-ENWPAFAPLIQDIKDSLPLLED 207

Query: 129 VSFVKCPRTANGIAHSLARSAAGFQPNFSP 159
              V  PR  NG+A  +A+ +  F+ N+ P
Sbjct: 208 FKIVYTPRGCNGVADRVAKESLSFE-NYDP 232

[Close]
BLAST of Spg032223 vs. ExPASy TrEMBL
Match: A0A1J3C7A8 (Putative ribonuclease H protein (Fragment) OS=Noccaea caerulescens OX=107243 GN=GA_TR12511_c0_g1_i1_g.40014 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 4.0e-11
Identity = 50/146 (34.25%), Postives = 79/146 (54.11%), Query Frame = 0 

Query: 13  WTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEATTIN 72
           W PPP G LK NVD +W+++  R GIGW++RDS+G  + MG+   RK  ++ S  AT I 
Sbjct: 587 WKPPPQGWLKCNVDGTWSEEHDRSGIGWVLRDSRGKVIWMGA---RKTKNLGSPLATEIE 646

Query: 73  ESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEVSFV 132
               A   +T    S II E++S E + A++ + A     + I ++I +  + +     V
Sbjct: 647 ALRWAIHFTTSLKCSNIIYETDSKETLEAISVD-AGWPAFKAIAKDISVMLTQLERKILV 706

Query: 133 KCPRTANGIAHSLARSAAGFQPNFSP 159
             PR  NG+A  +A+    F+ N+ P
Sbjct: 707 FQPREGNGVADRIAKETISFE-NYVP 727

[Close]
BLAST of Spg032223 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 5.2e-11
Identity = 50/137 (36.50%), Postives = 69/137 (50.36%), Query Frame = 0 

Query: 13  WTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEATTIN 72
           W PP     K+N +A+W  D   GGIGWI+RD +G  +      IR   +I  LE   I 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 73  ESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEVSFV 132
           E L A     +E    I LES+S+E +  L+ +  +++E   +LEEI      +  VS  
Sbjct: 139 EGLRAI---RQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMR 198

Query: 133 KCPRTANGIAHSLARSA 150
              R AN +AH LAR A
Sbjct: 199 HISREANKVAHGLARRA 212

[Close]
BLAST of Spg032223 vs. ExPASy TrEMBL
Match: A0A6J5UAY2 (Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS20161 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 6.8e-11
Identity = 43/134 (32.09%), Postives = 69/134 (51.49%), Query Frame = 0 

Query: 13   WTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEATTIN 72
            W  PP G+LK+N DA+W    + GG+GW++RDS G  V  G     +  S  ++EA  I 
Sbjct: 1147 WRKPPYGVLKVNCDAAWVSQTQMGGVGWVVRDSDGWMVQAGGKGDMRGGSALAMEAEAIR 1206

Query: 73   ESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEVSFV 132
            E+L   +A  +   S++ +ES+S++V+R +  E   +     I+ +I           F+
Sbjct: 1207 EAL---VACVQSGLSKLEVESDSLQVIRMIGGEWNMDIAVDTIVFDIKQLVGQFQHCVFL 1266

Query: 133  KCPRTANGIAHSLA 147
              PR  N  AH +A
Sbjct: 1267 YTPRNCNKAAHKIA 1277

[Close]
BLAST of Spg032223 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 1.5e-13
Identity = 47/146 (32.19%), Postives = 75/146 (51.37%), Query Frame = 0 

Query: 13  WTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEATTIN 72
           W PPP   +K N DA+WN D  R GIGW++R+ +G    MG+  + K  S+   E   + 
Sbjct: 420 WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMR 479

Query: 73  ESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEVSFV 132
               A L+ +R   + +I ES+S  ++  LN++    S  +  ++++    S   EV FV
Sbjct: 480 ---WAVLSLSRFQYNYVIFESDSQVLIEILNNDEIWPS-LKPTIQDLQRLLSQFTEVKFV 539

Query: 133 KCPRTANGIAHSLARSAAGFQPNFSP 159
             PR  N +A  +AR +  F  N+ P
Sbjct: 540 FIPREGNTLAERVARESLSFL-NYDP 560

[Close]
BLAST of Spg032223 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 3.3e-10
Identity = 44/146 (30.14%), Postives = 70/146 (47.95%), Query Frame = 0 

Query: 13  WTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEATTIN 72
           W  PP   +K N DA+W  +  R GIGWI+R+  G  + MG+  + +  ++   E   + 
Sbjct: 137 WKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALR 196

Query: 73  ESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEVSFV 132
               A L  +R +  +II ES++  +V  LN +    +  +  LE+I        EV F 
Sbjct: 197 ---WAVLTMSRFNYKRIIFESDAQALVNLLNSDDFWPT-LQPALEDIQQLLHHFEEVKFE 256

Query: 133 KCPRTANGIAHSLARSAAGFQPNFSP 159
             PR  N +A  +AR +  F  N+ P
Sbjct: 257 FTPRGGNKVADRIARESISFS-NYDP 277

[Close]
BLAST of Spg032223 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.2e-07
Identity = 39/137 (28.47%), Postives = 65/137 (47.45%), Query Frame = 0 

Query: 13  WTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEATTIN 72
           W+PP    LK N DAS ++     G+GWI+R+SQG+ +  G  + + R +    E +T+ 
Sbjct: 105 WSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTL- 164

Query: 73  ESLEAYLASTRESRSQIILESESIEVVRALNHEIANESETRVILEEIDLKASLIGEVSFV 132
             + A  AS      ++I E ++  + R +N + +N    +  L+ I         + F 
Sbjct: 165 --IWAIQASYGFGHKKVIFEGDNQTITRMINTKSSN-PRLQHFLDTIQSWIPSFESIEFS 224

Query: 133 KCPRTANGIAHSLARSA 150
              R  NG A  LA+ A
Sbjct: 225 FKHREQNGCADFLAKQA 237

[Close]
BLAST of Spg032223 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.6e-07
Identity = 45/158 (28.48%), Postives = 74/158 (46.84%), Query Frame = 0 

Query: 3   TSESQENQEIWTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWS 62
           T +  EN+  W  PP   +K N DA ++  +     GWIIR+  G+P+  GS ++    +
Sbjct: 316 TRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKLAHTSN 375

Query: 63  ICSLEATTINESLEAYLAST-RESRSQIILESESIEVVRALNHEIANESETRVILEEIDL 122
              LEA T  ++L A L  T     +Q+ +E +   ++  +N  I+  S     LE+I  
Sbjct: 376 --PLEAET--KALLAALQQTWIRGYTQVFMEGDCQTLINLIN-GISFHSSLANHLEDISF 435

Query: 123 KASLIGEVSFVKCPRTANGIAHSLARSAAGFQPNFSPS 160
            A+    + F    R  N +AH LA+    +   +S S
Sbjct: 436 WANKFASIQFGFIRRKGNKLAHVLAKYGCTYSTFYSGS 468

[Close]
BLAST of Spg032223 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 6.5e-06
Identity = 47/159 (29.56%), Postives = 67/159 (42.14%), Query Frame = 0 

Query: 13  WTPPPVGILKINVDASWNDDQRRGGIGWIIRDSQGSPVGMGSARIRKRWSICSLEATTIN 72
           W  PP   +K N D ++N   R    GW++RD  G  +G G A       I S  + ++ 
Sbjct: 186 WQRPPSTWIKYNYDGAFNHQTRNAKAGWLMRDENGVYMGSGQA-------IGSTTSDSLE 245

Query: 73  ESLEAYLASTRESRSQ----IILESESIEVVRALNHEIANESETRVILEEIDLKASLIGE 132
              +A + + + + SQ    +I E +S +V   +N+E  N      I  E         E
Sbjct: 246 SEFQALIIAMQHAWSQGYRKVIFEGDSKQVEELMNNEKLNFGRFNWI-REGRFWQKRFEE 305

Query: 133 VSFVKCPRTANGIAHSLARSAAGFQPNFSPSFVVEHAYV 168
             F   PRT N  A  LA+     QPN S  F   H YV
Sbjct: 306 AVFKWVPRTNNQPADILAKH--HLQPNQSFKF---HYYV 331

The following BLAST results are available for this feature:
BLAST of Spg032223 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_015384077.12.6e-1233.54uncharacterized protein LOC107176301 [Citrus sinensis] >XP_024039330.1 uncharact... [more]
PNT77801.13.4e-1233.56hypothetical protein BRADI_1g69052v3, partial [Brachypodium distachyon][more]
EOA22050.16.3e-1130.00hypothetical protein CARUB_v10002590mg, partial [Capsella rubella][more]
XP_006471930.18.3e-1130.41uncharacterized protein LOC102617744 [Citrus sinensis][more]
XP_022143535.11.1e-1036.50uncharacterized protein LOC111013412 [Momordica charantia][more]
BLAST of Spg032223 vs. NCBI nr
Match NameE-valueIdentityDescription
BLAST of Spg032223 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A2K2DU381.6e-1233.56RNase H domain-containing protein (Fragment) OS=Brachypodium distachyon OX=15368... [more]
R0HAP33.1e-1130.00RNase H domain-containing protein (Fragment) OS=Capsella rubella OX=81985 GN=CAR... [more]
A0A1J3C7A84.0e-1134.25Putative ribonuclease H protein (Fragment) OS=Noccaea caerulescens OX=107243 GN=... [more]
A0A6J1CP265.2e-1136.50uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013... [more]
A0A6J5UAY26.8e-1132.09Reverse transcriptase domain-containing protein OS=Prunus armeniaca OX=36596 GN=... [more]
BLAST of Spg032223 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G29090.11.5e-1332.19Ribonuclease H-like superfamily protein [more]
AT2G34320.13.3e-1030.14Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G65005.11.2e-0728.47Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G09510.11.6e-0728.48Ribonuclease H-like superfamily protein [more]
AT3G25270.16.5e-0629.56Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 24..149
e-value: 1.1E-14
score: 54.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 20..151
e-value: 1.1E-11
score: 46.8
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 9..151
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 9..151
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 23..147
e-value: 5.27843E-19
score: 77.7396
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 20..155

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg032223.1Spg032223.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity