Spg032113 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg032113
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionP-loop nucleoside triphosphate hydrolase superfamily protein
Locationscaffold11: 39967042 .. 39969991 (-)
RNA-Seq ExpressionSpg032113
SyntenySpg032113
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGACGAACAATCGAAGATCCTCATCCTCTCCCTCCGATCTCTCTGCTTTTTGAATTTTCCATCATGAAGCACGAAGGTAGTCGAAGCTACTTCCCAGCAATCCTTTGATTTTGTTCTTTGAAATTCGCTGAATGATGAAGATAACTCTCTTGCTAATTGCTATGTGCTTAGATGGCGGTTTCGGGAAAGTCCTTCCATCGTCGCACTATCTCAGCCCTTCGTTCGTCGGAGGCGTCGCCGCCGCCGGAAAGCGCCATTTCCGCCGGACTTCGAAGGCTGTGACCAACAATCATAAGTTGATTCCGCCGCATCTACATAGGACGGAGTCCGGCCGTGTTGATTTTTTGGAGAGATTTTCTCATTATGTTGGTAAAATCCTCCGCCATTACTGACTTGAAAATTAGTTCTATGTAAAAGGGGTAATCTGACCCTTCTATTTGGGTTTTTTTTTGTTTGGACTTTTGCAGCGAGGCAATTGGGGATTTCAGATGCAGATGAGTATCCTCAACTCTGCAAGTTAGCGAACAACTATTTGAGGAAGACGAAAGGGTGCGAGGAAGAGATTTATGCATATTTCGCGAGTGAATCCGAGGCAGAATCTCTGTATGTGAAGTTAATGGATGAGTTCGATCAATGCATCTTGAGTTATTTCGCATTCCATTGGAGCCAAGCTCCCTTGATGATCACTCAGGTAAAATTCATCAATCTCGAAAGAAAAGGTCAACTCGATGTTAAAATTTCATCATATCCCTTAATTAATGGCGGATTTAATGATAGGTTTTGGGTGTTGACTCCGAGCACAAGAAGCTGAAAGACCTTGTGGTGGCAGCGACTAGGTAATTTCCTTTTTCATCTTTGTCAATTTGCTTGAGAAGTCAATTTTGGTTCCTGAGTTTGAACTTTAAAACACACCAGCATCCTTTTCTTGAATCCTGATGCATCTTGTAGGATTAAGATCCACTAGATAACTTCAATAAGGGAAGATTCTGAGTCTTTTCTTTTCCTTTTTTTGGTTTCTGGCTGTTTTTATAGGAAGCAAAGATTCGATAGGGTGAGTAAGGATCTGAAAGTGACAAGGGTGTTCTCTACTTTGGTGGAGGAGATGAAGAAGATTGGCTGTGCTCCATCTAAGGGCGAGTCGAAATGCTCCCACACGATACCGCCATGGTCTCAAAGAGAGAGGAGCCCTGTGCTTCTTCTTATGGGTGGTGGCATGGGGGCTGGCAAGAGTACTGTCCTCAAAGACATTCGCAAAGAGTGAGTAATATCGATATCGAATCCTGCTAATTTGGCAAAACATAAGAACTTTTAGAACAAAGTTTGTTGATAAACAGTTGCTAATTATGCAGGCCCTTCTGGCTGGAAGCAGATACAAACGTAGTGAATGTAGAGGCAGATGCCTTCAAAGAGACTGATGTTATCTACAAAGCCATCAGCTCGATGGGCTATCACGACGACATGCTTCAGACTGCTGAATTGGTGAGTATCTCTCCTCTTCAGCAGAACCACACATTTAATTACATCTCTAGTTGTTTCCATGAATAAACTAACTTACTAACTACGTGATGAATTACAACAGGTACACCAGCCATCAATTGATGCTGCATCTTCGTTGCTGGTCACAGCATTAAACGAGGGACGGGATGTGATCTTGGACAGCACTCTGTCATGGGAGCCATATGTTATGCAGACGATTGAAATGGCTCGAAACATTCACAATCGTCGATATCGGATGGGAGTTGGGTACAAGGTAGAGAATGGTAATGTCACAGAGAATTACTGGGAGCCTGTGAGTGAAGAGGAAGAGGAGGAATTGAGGAAAGAGCATGGAGAAATGCAAAATAGAAGACCATATAGAATAGAGCTGGTTGGAGTTGTTTGTGATGCTCATCTAGCTGTTGTTAGAGGAATAAGGTAAGAATACAAGTCAACTTTTGGAGCTTTTGTATTGACATCGTAAGGCATATATAGTTCTAATGAAGACTGATGATTTGAACAGGAGAGCCATAATGATGGGAAGAGCTGTGAGGGTGAATTCTCAGTTGCAATCCCACAAGAGATTTGCCAATGCTTTCCCAAAATACTCTGAAGTTGTTGATAGTGTCAGGCTATATTCTACCAATTCCATTGGCAACCCACCTAAGGTATTTTAAAATTATTTGTTGAATCTTGAACATTATATATGAAATTTAGATTATACTCTCTAATAGAATATTGACATGACATGTCTATTAAACAAATTTTAATTGAGTTGGATAGACATGATAAATATTTGAATAAACTAATCAAAGCATGTATGATTGTATTAATAGTGTCAAAAACTTGGATTAAATATAAGATGTAATTAAAACTTTCTAAAGACACAAATACCAAATTTATAATATAACTTTAATTTTGTTGTTGTACTTTTTACTCCTTTATGATTCCTACTTTGAATTAATTACTTGTTGTATTTCCAAGAAACTGTGACATTGCAGGTGTATTAATTATTTAAAACGTGTTTCCAATTTCGCAGTTGATCCATAGAAAAGACAACGACGACCCATTTGAGATGGTTGACCCGGAAGCACTTGCGTGTTTGACGGCGTTGAGCAATTTGAACCCGGATGCGGAATCCGTTTACGAGCTTTACCCGAACCCGAACCCATTTTCAGAACCCGAGCCCATTTTGAAAGAAATTGCTCTGACCCCTTCAAGGGTCCAGTCTCAGAAAGAGCTAAGATCTGCGATTCAAAAGCTAGAAAGTTCAAGAACAATCTCAAATGAAATTGAACAGTGAAAAACTTGTAAATTTCTTGGTGGTTGAAGTGAAGTTATATTAATGATATGGTAATCTTCACTTTCTTGAAAGAAGAAGAAGAAGAACCATTTGTAGTTTTGATTAATCTAACAAACGCAAGACTTCTTTTTTTTTTTTTTTGCTCGTTCATAAGAATGTTGAAACTCTGTG

mRNA sequence

ATGATGAAGATAACTCTCTTGCTAATTGCTATGTGCTTAGATGGCGGTTTCGGGAAAGTCCTTCCATCGTCGCACTATCTCAGCCCTTCGTTCGTCGGAGGCGTCGCCGCCGCCGGAAAGCGCCATTTCCGCCGGACTTCGAAGGCTGTGACCAACAATCATAAGTTGATTCCGCCGCATCTACATAGGACGGAGTCCGGCCGTGTTGATTTTTTGGAGAGATTTTCTCATTATGTTGCGAGGCAATTGGGGATTTCAGATGCAGATGAGTATCCTCAACTCTGCAAGTTAGCGAACAACTATTTGAGGAAGACGAAAGGGTGCGAGGAAGAGATTTATGCATATTTCGCGAGTGAATCCGAGGCAGAATCTCTGTATGTGAAGTTAATGGATGAGTTCGATCAATGCATCTTGAGTTATTTCGCATTCCATTGGAGCCAAGCTCCCTTGATGATCACTCAGGTTTTGGGTGTTGACTCCGAGCACAAGAAGCTGAAAGACCTTGTGGTGGCAGCGACTAGGAAGCAAAGATTCGATAGGGTGAGTAAGGATCTGAAAGTGACAAGGGTGTTCTCTACTTTGGTGGAGGAGATGAAGAAGATTGGCTGTGCTCCATCTAAGGGCGAGTCGAAATGCTCCCACACGATACCGCCATGGTCTCAAAGAGAGAGGAGCCCTGTGCTTCTTCTTATGGGTGGTGGCATGGGGGCTGGCAAGAGTACTGTCCTCAAAGACATTCGCAAAGAGCCCTTCTGGCTGGAAGCAGATACAAACGTAGTGAATGTAGAGGCAGATGCCTTCAAAGAGACTGATGTTATCTACAAAGCCATCAGCTCGATGGGCTATCACGACGACATGCTTCAGACTGCTGAATTGGTACACCAGCCATCAATTGATGCTGCATCTTCGTTGCTGGTCACAGCATTAAACGAGGGACGGGATGTGATCTTGGACAGCACTCTGTCATGGGAGCCATATGTTATGCAGACGATTGAAATGGCTCGAAACATTCACAATCGTCGATATCGGATGGGAGTTGGGTACAAGGTAGAGAATGGTAATGTCACAGAGAATTACTGGGAGCCTGTGAGTGAAGAGGAAGAGGAGGAATTGAGGAAAGAGCATGGAGAAATGCAAAATAGAAGACCATATAGAATAGAGCTGGTTGGAGTTGTTTGTGATGCTCATCTAGCTGTTGTTAGAGGAATAAGGAGAGCCATAATGATGGGAAGAGCTGTGAGGGTGAATTCTCAGTTGCAATCCCACAAGAGATTTGCCAATGCTTTCCCAAAATACTCTGAAGTTGTTGATAGTGTCAGGCTATATTCTACCAATTCCATTGGCAACCCACCTAAGTTGATCCATAGAAAAGACAACGACGACCCATTTGAGATGGTTGACCCGGAAGCACTTGCGTGTTTGACGGCGTTGAGCAATTTGAACCCGGATGCGGAATCCGTTTACGAGCTTTACCCGAACCCGAACCCATTTTCAGAACCCGAGCCCATTTTGAAAGAAATTGCTCTGACCCCTTCAAGGGTCCAGTCTCAGAAAGAGCTAAGATCTGCGATTCAAAAGCTAGAAAGTTCAAGAACAATCTCAAATGAAATTGAACAGTGA

Coding sequence (CDS)

ATGATGAAGATAACTCTCTTGCTAATTGCTATGTGCTTAGATGGCGGTTTCGGGAAAGTCCTTCCATCGTCGCACTATCTCAGCCCTTCGTTCGTCGGAGGCGTCGCCGCCGCCGGAAAGCGCCATTTCCGCCGGACTTCGAAGGCTGTGACCAACAATCATAAGTTGATTCCGCCGCATCTACATAGGACGGAGTCCGGCCGTGTTGATTTTTTGGAGAGATTTTCTCATTATGTTGCGAGGCAATTGGGGATTTCAGATGCAGATGAGTATCCTCAACTCTGCAAGTTAGCGAACAACTATTTGAGGAAGACGAAAGGGTGCGAGGAAGAGATTTATGCATATTTCGCGAGTGAATCCGAGGCAGAATCTCTGTATGTGAAGTTAATGGATGAGTTCGATCAATGCATCTTGAGTTATTTCGCATTCCATTGGAGCCAAGCTCCCTTGATGATCACTCAGGTTTTGGGTGTTGACTCCGAGCACAAGAAGCTGAAAGACCTTGTGGTGGCAGCGACTAGGAAGCAAAGATTCGATAGGGTGAGTAAGGATCTGAAAGTGACAAGGGTGTTCTCTACTTTGGTGGAGGAGATGAAGAAGATTGGCTGTGCTCCATCTAAGGGCGAGTCGAAATGCTCCCACACGATACCGCCATGGTCTCAAAGAGAGAGGAGCCCTGTGCTTCTTCTTATGGGTGGTGGCATGGGGGCTGGCAAGAGTACTGTCCTCAAAGACATTCGCAAAGAGCCCTTCTGGCTGGAAGCAGATACAAACGTAGTGAATGTAGAGGCAGATGCCTTCAAAGAGACTGATGTTATCTACAAAGCCATCAGCTCGATGGGCTATCACGACGACATGCTTCAGACTGCTGAATTGGTACACCAGCCATCAATTGATGCTGCATCTTCGTTGCTGGTCACAGCATTAAACGAGGGACGGGATGTGATCTTGGACAGCACTCTGTCATGGGAGCCATATGTTATGCAGACGATTGAAATGGCTCGAAACATTCACAATCGTCGATATCGGATGGGAGTTGGGTACAAGGTAGAGAATGGTAATGTCACAGAGAATTACTGGGAGCCTGTGAGTGAAGAGGAAGAGGAGGAATTGAGGAAAGAGCATGGAGAAATGCAAAATAGAAGACCATATAGAATAGAGCTGGTTGGAGTTGTTTGTGATGCTCATCTAGCTGTTGTTAGAGGAATAAGGAGAGCCATAATGATGGGAAGAGCTGTGAGGGTGAATTCTCAGTTGCAATCCCACAAGAGATTTGCCAATGCTTTCCCAAAATACTCTGAAGTTGTTGATAGTGTCAGGCTATATTCTACCAATTCCATTGGCAACCCACCTAAGTTGATCCATAGAAAAGACAACGACGACCCATTTGAGATGGTTGACCCGGAAGCACTTGCGTGTTTGACGGCGTTGAGCAATTTGAACCCGGATGCGGAATCCGTTTACGAGCTTTACCCGAACCCGAACCCATTTTCAGAACCCGAGCCCATTTTGAAAGAAATTGCTCTGACCCCTTCAAGGGTCCAGTCTCAGAAAGAGCTAAGATCTGCGATTCAAAAGCTAGAAAGTTCAAGAACAATCTCAAATGAAATTGAACAGTGA

Protein sequence

MMKITLLLIAMCLDGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLERFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEFDQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWLEADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGRDVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRKEHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPNPNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ
Homology
BLAST of Spg032113 vs. NCBI nr
Match: KAG6582355.1 (Calmodulin calcium-dependent NAD kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 931.4 bits (2406), Expect = 3.5e-267
Identity = 477/526 (90.68%), Postives = 489/526 (92.97%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGKV+ SSHYL+ SFVGGV AAG RHFRRTSKAV NN KLI PHLHRTESGRVDFLE
Sbjct: 5   DGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLI-PHLHRTESGRVDFLE 64

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVARQLGISDADE PQLCKLANNYLRKTKGCEEEIYAYFASE++ ESLYVKLMDEF
Sbjct: 65  RFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLMDEF 124

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST
Sbjct: 125 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 184

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCSH  P  SQR+RSPVLLLMGGGMGAGKSTVLKDI KEPFWL
Sbjct: 185 LVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEPFWL 244

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 245 EAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 304

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSWEPYVMQTIEMARNIH RRYRMGVGYKVE  NVTENYWEPVSEEEEEELRK
Sbjct: 305 DVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEELRK 364

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
           EHGEMQNR PYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS
Sbjct: 365 EHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 424

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           EVVDSVRLYSTNS G PPKLIHRK+ DDPF+M DPEA ACL+ LSNLNPDAESVYELYPN
Sbjct: 425 EVVDSVRLYSTNSFGKPPKLIHRKEGDDPFQMFDPEANACLSTLSNLNPDAESVYELYPN 484

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
           P+ FSEPE I KEIA TPSRV SQK+LRS I+K+ESSR ISNEI Q
Sbjct: 485 PSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKVESSRRISNEIVQ 529

BLAST of Spg032113 vs. NCBI nr
Match: XP_022924243.1 (uncharacterized protein LOC111431771 [Cucurbita moschata])

HSP 1 Score: 927.5 bits (2396), Expect = 5.1e-266
Identity = 476/526 (90.49%), Postives = 488/526 (92.78%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGKV+ SSHYL+ SFVGGV AAG RHFRRTSKAV NN KLI PHLHRTESGRVDFLE
Sbjct: 5   DGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLI-PHLHRTESGRVDFLE 64

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVARQLGISDADE PQLCKLANNYLRKTKGCEEEIYAYFASE++ ESLYVKLMDEF
Sbjct: 65  RFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLMDEF 124

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST
Sbjct: 125 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 184

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCSH  P  SQR+RSPVLLLMGGGMGAGKSTVLKDI KEPFWL
Sbjct: 185 LVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEPFWL 244

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 245 EAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 304

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSWEPYVMQTIEMARNIH RRYRMGVGYKVE  NVTENYWEPVSEEEEEELRK
Sbjct: 305 DVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEELRK 364

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
           EHGEMQNR PYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS
Sbjct: 365 EHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 424

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           EVVDSVRLYSTNS G PPKLIHRK+ DDPF+M DPEA ACL+ LSNLNPDAESVYELYPN
Sbjct: 425 EVVDSVRLYSTNSFGKPPKLIHRKEGDDPFQMFDPEANACLSTLSNLNPDAESVYELYPN 484

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
            + FSEPE I KEIA TPSRV SQK+LRS I+K+ESSR ISNEI Q
Sbjct: 485 LSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKVESSRRISNEIVQ 529

BLAST of Spg032113 vs. NCBI nr
Match: XP_023526441.1 (uncharacterized protein LOC111789938 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924.9 bits (2389), Expect = 3.3e-265
Identity = 474/526 (90.11%), Postives = 488/526 (92.78%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGKV+ SSHYL+ SFVGGV AAG RHFRRTSKAV NN KLI PHLHRTESGRVDFLE
Sbjct: 5   DGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLI-PHLHRTESGRVDFLE 64

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVARQLGISDAD+ PQLCKLANNYLRKTKGCEEEIYAYFASE++ ESLYVKLMDEF
Sbjct: 65  RFSHYVARQLGISDADDCPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLMDEF 124

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST
Sbjct: 125 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 184

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCSH  P  SQR+RSPVLLLMGGGMGAGKSTVLKDI KEPFWL
Sbjct: 185 LVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEPFWL 244

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 245 EAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 304

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSWEPYVMQTIEMARNIH RRYRMGVGYKVE+  VTENYWEPVSEEEEEELRK
Sbjct: 305 DVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVESDKVTENYWEPVSEEEEEELRK 364

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
           EHGEMQNR PYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS
Sbjct: 365 EHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 424

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           +VVDSVRLYSTNS G PPKLIHRK+ DDPF+M D EA ACL+ LSNLNPDAESVYELYPN
Sbjct: 425 KVVDSVRLYSTNSFGKPPKLIHRKEGDDPFQMFDSEANACLSTLSNLNPDAESVYELYPN 484

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
           P+ FSEPE I KEIA TPSRV SQK+LRS I+KLESSR ISNEI Q
Sbjct: 485 PSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKLESSRRISNEIVQ 529

BLAST of Spg032113 vs. NCBI nr
Match: XP_022980774.1 (uncharacterized protein LOC111480063 [Cucurbita maxima])

HSP 1 Score: 924.9 bits (2389), Expect = 3.3e-265
Identity = 474/526 (90.11%), Postives = 486/526 (92.40%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGKV+ SSHYL+ SFVGGV AAG RHFRRTSKAV NN KLI PHLHRTESGRVDFLE
Sbjct: 5   DGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLI-PHLHRTESGRVDFLE 64

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVARQLGISDADE PQLCKLANNYLRKTKGCEEEIYAYFASE++ ESLYVKLMDEF
Sbjct: 65  RFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLMDEF 124

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST
Sbjct: 125 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 184

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCSH  P  SQR+RSPVLLLMGGGMGAGKSTVLKDI KEPFWL
Sbjct: 185 LVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEPFWL 244

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 245 EAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 304

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSWEPYVMQTIEMARNIH RRYRMGVGYKVE  NVTENYWEPVSEEEEEELR 
Sbjct: 305 DVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEELRN 364

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
           EHGEMQNR PYRIELVGVVCDAHLAVVRGIRRAIM+GRAVRVNSQLQSHKRFANAFPKYS
Sbjct: 365 EHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMVGRAVRVNSQLQSHKRFANAFPKYS 424

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           EVVDSVRLYSTNS G PPKLIHRK+ DDPF+M DPEA ACL+ LS LNPDAESVYELYPN
Sbjct: 425 EVVDSVRLYSTNSFGKPPKLIHRKEGDDPFQMFDPEANACLSTLSKLNPDAESVYELYPN 484

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
           P+ FSEPE I KEIA TPSRV SQK+LRS I+KLESSR I NEI Q
Sbjct: 485 PSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKLESSRRICNEIVQ 529

BLAST of Spg032113 vs. NCBI nr
Match: XP_038877094.1 (uncharacterized protein LOC120069426 [Benincasa hispida])

HSP 1 Score: 922.9 bits (2384), Expect = 1.2e-264
Identity = 473/526 (89.92%), Postives = 488/526 (92.78%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGKV+PSSHYL+PSFVGGV AAGKRHFRRTSK V NN KLI PHL RTESGRVDFLE
Sbjct: 5   DGGFGKVIPSSHYLNPSFVGGVPAAGKRHFRRTSKVVINNQKLI-PHLRRTESGRVDFLE 64

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVAR LG SD DE PQLCKLANNYLRKTKGCEEEIYAYFASESE ESLYVKL++EF
Sbjct: 65  RFSHYVARLLGFSDVDECPQLCKLANNYLRKTKGCEEEIYAYFASESEGESLYVKLVNEF 124

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST
Sbjct: 125 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 184

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCS T P   QRER+PVLLLMGGGMGAGKSTVLKDI KEPFWL
Sbjct: 185 LVEEMKKIGCASSKGESKCSLTAPRSCQRERNPVLLLMGGGMGAGKSTVLKDILKEPFWL 244

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA+TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 245 EAETNTVTVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 304

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSWEPYVMQTIEMARNIH  RYRMGVGYKVENGNVTENYWEPVSEEEE+    
Sbjct: 305 DVILDSTLSWEPYVMQTIEMARNIHKCRYRMGVGYKVENGNVTENYWEPVSEEEED---- 364

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
             GEMQNR PYRIELVGVVCDAHLAVVRGIRRAI+MGRAVRVNSQLQSHKRFA+AFPKYS
Sbjct: 365 --GEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIVMGRAVRVNSQLQSHKRFAHAFPKYS 424

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           EVVDSVRLYSTNSIGNPPKLIHRKD DDPF+M+DPEA ACLT LSNLNPDAESVYELYPN
Sbjct: 425 EVVDSVRLYSTNSIGNPPKLIHRKDGDDPFQMIDPEANACLTTLSNLNPDAESVYELYPN 484

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
           P+PFSEPE I KEIALTPSR+  QKELRSAI+KLESSRT+SNEI Q
Sbjct: 485 PSPFSEPEAIWKEIALTPSRLHPQKELRSAIKKLESSRTVSNEIVQ 523

BLAST of Spg032113 vs. ExPASy Swiss-Prot
Match: Q0WUY1 (Calmodulin calcium-dependent NAD kinase OS=Arabidopsis thaliana OX=3702 GN=NADKC PE=1 SV=1)

HSP 1 Score: 583.6 bits (1503), Expect = 2.3e-165
Identity = 309/513 (60.23%), Postives = 385/513 (75.05%), Query Frame = 0

Query: 32  VGGVAAAGKRHF--------RRTSKAVTNNHK--LIPPHLHRTESGRVDFLERFSHYVAR 91
           +GG+AAA    +        R+  +  +  +K   I P + R +SGR   LERFSHYVAR
Sbjct: 27  LGGLAAAVAAAYAGELLLRRRKLDQGASMGYKDVKIAPLIERKDSGRRSNLERFSHYVAR 86

Query: 92  QLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEFDQCILSYF 151
           QLG  D +EYPQLCKLAN YL KTKG +E +  Y  +E+E +SLYV L++EFD+CIL+YF
Sbjct: 87  QLGFEDPNEYPQLCKLANGYLLKTKGYDENVDEYLENEAERDSLYVHLLEEFDRCILTYF 146

Query: 152 AFHWSQAPLMITQVLGVDSEHK--KLKDLVVAATRKQRFDRVSKDLKVTRVFSTLVEEMK 211
           +F+W+Q+  +I+Q L  +S+ K  KLKD V+AATRKQRF+RV+KDLKV RV STLVEEM+
Sbjct: 147 SFNWTQSSNLISQALSDESDQKVPKLKDFVMAATRKQRFERVTKDLKVKRVISTLVEEMR 206

Query: 212 KIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWLEADTNV 271
            IG   S  E  C+  + P +  +RSPVLLLMGGGMGAGKSTVLKDI  E FW EA  + 
Sbjct: 207 VIGSGSS--EPHCTEVMSPVAHNKRSPVLLLMGGGMGAGKSTVLKDIFLESFWSEAQADA 266

Query: 272 VNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGRDVILDS 331
           V +EADAFKETDVIY+A+SS G+HDDMLQTAELVHQ S DAASSLLVTALN+GRDVI+D 
Sbjct: 267 VVIEADAFKETDVIYRALSSRGHHDDMLQTAELVHQSSTDAASSLLVTALNDGRDVIMDG 326

Query: 332 TLSWEPYVMQTIEMARNIHNRRYRMGVGYKV-ENGNVTENYWEPVSEEEEEELRKEHGEM 391
           TLSWEP+V Q IEMARN+H ++YRMG GYKV E G +TE YW     EEEEE  KE+G+ 
Sbjct: 327 TLSWEPFVEQMIEMARNVHKQKYRMGEGYKVSEEGTITEKYW-----EEEEEETKENGKQ 386

Query: 392 QNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYSEVVDS 451
           QN +PYRIELVGVVCDA+LAV RGIRRA+M+ RAVRV  QL SHKRFANAFPKY E+VD+
Sbjct: 387 QNLKPYRIELVGVVCDAYLAVARGIRRALMVKRAVRVKPQLNSHKRFANAFPKYCELVDN 446

Query: 452 VRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPNPNPFS 511
            RLY TN++G PP+LI  KD +    +VDPE + CL  +S+LNPDAES+YELYP+P+  S
Sbjct: 447 ARLYCTNAVGGPPRLIAWKDGNSKL-LVDPEDIDCLKRVSSLNPDAESIYELYPDPSQLS 506

Query: 512 EPEPILKEIALTPSRVQSQKELRSAIQKLESSR 532
           +P  +  ++ L PSR + QKEL  AI+++E ++
Sbjct: 507 KPGSVWNDVVLVPSRPKVQKELSDAIRRIEKAQ 531

BLAST of Spg032113 vs. ExPASy TrEMBL
Match: A0A6J1EBV0 (uncharacterized protein LOC111431771 OS=Cucurbita moschata OX=3662 GN=LOC111431771 PE=4 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.4e-266
Identity = 476/526 (90.49%), Postives = 488/526 (92.78%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGKV+ SSHYL+ SFVGGV AAG RHFRRTSKAV NN KLI PHLHRTESGRVDFLE
Sbjct: 5   DGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLI-PHLHRTESGRVDFLE 64

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVARQLGISDADE PQLCKLANNYLRKTKGCEEEIYAYFASE++ ESLYVKLMDEF
Sbjct: 65  RFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLMDEF 124

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST
Sbjct: 125 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 184

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCSH  P  SQR+RSPVLLLMGGGMGAGKSTVLKDI KEPFWL
Sbjct: 185 LVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEPFWL 244

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 245 EAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 304

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSWEPYVMQTIEMARNIH RRYRMGVGYKVE  NVTENYWEPVSEEEEEELRK
Sbjct: 305 DVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEELRK 364

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
           EHGEMQNR PYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS
Sbjct: 365 EHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 424

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           EVVDSVRLYSTNS G PPKLIHRK+ DDPF+M DPEA ACL+ LSNLNPDAESVYELYPN
Sbjct: 425 EVVDSVRLYSTNSFGKPPKLIHRKEGDDPFQMFDPEANACLSTLSNLNPDAESVYELYPN 484

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
            + FSEPE I KEIA TPSRV SQK+LRS I+K+ESSR ISNEI Q
Sbjct: 485 LSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKVESSRRISNEIVQ 529

BLAST of Spg032113 vs. ExPASy TrEMBL
Match: A0A6J1J066 (uncharacterized protein LOC111480063 OS=Cucurbita maxima OX=3661 GN=LOC111480063 PE=4 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.6e-265
Identity = 474/526 (90.11%), Postives = 486/526 (92.40%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGKV+ SSHYL+ SFVGGV AAG RHFRRTSKAV NN KLI PHLHRTESGRVDFLE
Sbjct: 5   DGGFGKVITSSHYLNTSFVGGVPAAGSRHFRRTSKAVINNQKLI-PHLHRTESGRVDFLE 64

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVARQLGISDADE PQLCKLANNYLRKTKGCEEEIYAYFASE++ ESLYVKLMDEF
Sbjct: 65  RFSHYVARQLGISDADECPQLCKLANNYLRKTKGCEEEIYAYFASEADGESLYVKLMDEF 124

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST
Sbjct: 125 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 184

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCSH  P  SQR+RSPVLLLMGGGMGAGKSTVLKDI KEPFWL
Sbjct: 185 LVEEMKKIGCASSKGESKCSHATPQLSQRKRSPVLLLMGGGMGAGKSTVLKDILKEPFWL 244

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 245 EAKTNRVMVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 304

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSWEPYVMQTIEMARNIH RRYRMGVGYKVE  NVTENYWEPVSEEEEEELR 
Sbjct: 305 DVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVEKDNVTENYWEPVSEEEEEELRN 364

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
           EHGEMQNR PYRIELVGVVCDAHLAVVRGIRRAIM+GRAVRVNSQLQSHKRFANAFPKYS
Sbjct: 365 EHGEMQNRMPYRIELVGVVCDAHLAVVRGIRRAIMVGRAVRVNSQLQSHKRFANAFPKYS 424

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           EVVDSVRLYSTNS G PPKLIHRK+ DDPF+M DPEA ACL+ LS LNPDAESVYELYPN
Sbjct: 425 EVVDSVRLYSTNSFGKPPKLIHRKEGDDPFQMFDPEANACLSTLSKLNPDAESVYELYPN 484

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
           P+ FSEPE I KEIA TPSRV SQK+LRS I+KLESSR I NEI Q
Sbjct: 485 PSQFSEPEAIWKEIAPTPSRVHSQKQLRSEIKKLESSRRICNEIVQ 529

BLAST of Spg032113 vs. ExPASy TrEMBL
Match: A0A5A7U1T8 (P-loop nucleoside triphosphate hydrolase superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001100 PE=4 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 2.4e-261
Identity = 466/526 (88.59%), Postives = 487/526 (92.59%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGKV+P+SHYL+ SFVGGV+A G RHFRRTSK V NN KLI PHL RTESGRVDFLE
Sbjct: 5   DGGFGKVIPTSHYLNTSFVGGVSATGNRHFRRTSKPVINNQKLI-PHLRRTESGRVDFLE 64

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVARQLGISD DE PQLCKLANNYLRKTKGCEEEIYAYFASE+E ESLYVKL++EF
Sbjct: 65  RFSHYVARQLGISDVDECPQLCKLANNYLRKTKGCEEEIYAYFASEAEGESLYVKLVNEF 124

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLK+TRVFST
Sbjct: 125 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKMTRVFST 184

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCS T P  SQR+RSPVLLLMGGGMGAGKSTVLKDI KEPFWL
Sbjct: 185 LVEEMKKIGCAASKGESKCSLTTPRSSQRQRSPVLLLMGGGMGAGKSTVLKDILKEPFWL 244

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA+TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 245 EAETNTVKVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 304

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSWEPYVMQTIEMARNIH RRYRMGVGYKVENG VTENYWEPVSEEEE+E   
Sbjct: 305 DVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVENGKVTENYWEPVSEEEEDE--- 364

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
              EMQ+R PYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRV+SQLQSHKRFANAFPKYS
Sbjct: 365 ---EMQDRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVSSQLQSHKRFANAFPKYS 424

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           EVVDSVRLYSTN IGNPPKLIHRKD  DPF+ +D EA ACLT LSNLNPDAESVYELYPN
Sbjct: 425 EVVDSVRLYSTNYIGNPPKLIHRKDGTDPFQTIDAEASACLTTLSNLNPDAESVYELYPN 484

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
           P+PFSEPE I KEIALTPSR+ SQKELRSAI+K+ESSRTIS+E+EQ
Sbjct: 485 PSPFSEPEAIWKEIALTPSRLHSQKELRSAIKKIESSRTISSEVEQ 523

BLAST of Spg032113 vs. ExPASy TrEMBL
Match: A0A0A0L4J6 (Zeta_toxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G143510 PE=4 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 3.1e-261
Identity = 466/526 (88.59%), Postives = 486/526 (92.40%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGK++P+SHYL+ SFVGGVAA G RHFRRTSK V NN KLI PHLHRTESGRVDFLE
Sbjct: 34  DGGFGKLIPTSHYLNQSFVGGVAATGNRHFRRTSKPVINNQKLI-PHLHRTESGRVDFLE 93

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVARQLGISD DE PQLCKLANNYLRKTKGCEEEIYAYFASE+  ESLYVKL+ EF
Sbjct: 94  RFSHYVARQLGISDVDECPQLCKLANNYLRKTKGCEEEIYAYFASEAGGESLYVKLVTEF 153

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLK+TRVFST
Sbjct: 154 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKMTRVFST 213

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCS T P  SQR+RSPVLL+MGGGMGAGKSTVLKDI KEPFWL
Sbjct: 214 LVEEMKKIGCAASKGESKCSLTKPRSSQRQRSPVLLMMGGGMGAGKSTVLKDILKEPFWL 273

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA+TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 274 EAETNTVTVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 333

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSW PYVMQTIEMARNIH RRYRMGVGYKVENG VTENYWEPVSEEEE+    
Sbjct: 334 DVILDSTLSWAPYVMQTIEMARNIHKRRYRMGVGYKVENGEVTENYWEPVSEEEED---- 393

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
             GEMQ+R PYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS
Sbjct: 394 --GEMQDRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 453

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           EVVDSVRLYSTN IGNPPKLIHRKD  DPF+ +D EA ACLT LSNLNPDAESVYELYPN
Sbjct: 454 EVVDSVRLYSTNFIGNPPKLIHRKDGTDPFQTIDAEASACLTTLSNLNPDAESVYELYPN 513

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
           P+PFSEPE I KEIALTPSR+QSQKELRSAI+KLESSRT+S++IEQ
Sbjct: 514 PSPFSEPEAIWKEIALTPSRLQSQKELRSAIKKLESSRTVSSKIEQ 552

BLAST of Spg032113 vs. ExPASy TrEMBL
Match: A0A1S3AXE1 (uncharacterized protein LOC103483643 OS=Cucumis melo OX=3656 GN=LOC103483643 PE=4 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 9.0e-261
Identity = 465/526 (88.40%), Postives = 487/526 (92.59%), Query Frame = 0

Query: 14  DGGFGKVLPSSHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLE 73
           DGGFGKV+P+SHYL+ SFVGGV+A G RHFRRTSK V NN KLI PHL RTESGRVDFLE
Sbjct: 5   DGGFGKVIPTSHYLNTSFVGGVSATGNRHFRRTSKPVINNQKLI-PHLRRTESGRVDFLE 64

Query: 74  RFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEF 133
           RFSHYVARQLGISD DE PQLCKLANNYLRKTKGCEEEIYAYFASE+E ESLYVKL++EF
Sbjct: 65  RFSHYVARQLGISDVDECPQLCKLANNYLRKTKGCEEEIYAYFASEAEGESLYVKLVNEF 124

Query: 134 DQCILSYFAFHWSQAPLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKVTRVFST 193
           DQCILSYFAFHWSQA LMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLK+TRVFST
Sbjct: 125 DQCILSYFAFHWSQASLMITQVLGVDSEHKKLKDLVVAATRKQRFDRVSKDLKMTRVFST 184

Query: 194 LVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWL 253
           LVEEMKKIGCA SKGESKCS T P  SQR+RSPVLLLMGGGMGAGKSTVLKDI KEPFWL
Sbjct: 185 LVEEMKKIGCAASKGESKCSLTTPRSSQRQRSPVLLLMGGGMGAGKSTVLKDILKEPFWL 244

Query: 254 EADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 313
           EA+TN V VEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR
Sbjct: 245 EAETNTVKVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGR 304

Query: 314 DVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKVENGNVTENYWEPVSEEEEEELRK 373
           DVILDSTLSWEPYVMQTIEMARNIH RRYRMGVGYKVENG VTENYWEPVSEEEE+E   
Sbjct: 305 DVILDSTLSWEPYVMQTIEMARNIHKRRYRMGVGYKVENGKVTENYWEPVSEEEEDE--- 364

Query: 374 EHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYS 433
              EMQ+R PYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRV+SQLQSHKRFANAFPK+S
Sbjct: 365 ---EMQDRMPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVSSQLQSHKRFANAFPKHS 424

Query: 434 EVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPN 493
           EVVDSVRLYSTN IGNPPKLIHRKD  DPF+ +D EA ACLT LSNLNPDAESVYELYPN
Sbjct: 425 EVVDSVRLYSTNYIGNPPKLIHRKDGTDPFQTIDAEASACLTTLSNLNPDAESVYELYPN 484

Query: 494 PNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLESSRTISNEIEQ 540
           P+PFSEPE I KEIALTPSR+ SQKELRSAI+K+ESSRTIS+E+EQ
Sbjct: 485 PSPFSEPEAIWKEIALTPSRLHSQKELRSAIKKIESSRTISSEVEQ 523

BLAST of Spg032113 vs. TAIR 10
Match: AT1G04280.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 583.6 bits (1503), Expect = 1.7e-166
Identity = 309/513 (60.23%), Postives = 385/513 (75.05%), Query Frame = 0

Query: 32  VGGVAAAGKRHF--------RRTSKAVTNNHK--LIPPHLHRTESGRVDFLERFSHYVAR 91
           +GG+AAA    +        R+  +  +  +K   I P + R +SGR   LERFSHYVAR
Sbjct: 27  LGGLAAAVAAAYAGELLLRRRKLDQGASMGYKDVKIAPLIERKDSGRRSNLERFSHYVAR 86

Query: 92  QLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEFDQCILSYF 151
           QLG  D +EYPQLCKLAN YL KTKG +E +  Y  +E+E +SLYV L++EFD+CIL+YF
Sbjct: 87  QLGFEDPNEYPQLCKLANGYLLKTKGYDENVDEYLENEAERDSLYVHLLEEFDRCILTYF 146

Query: 152 AFHWSQAPLMITQVLGVDSEHK--KLKDLVVAATRKQRFDRVSKDLKVTRVFSTLVEEMK 211
           +F+W+Q+  +I+Q L  +S+ K  KLKD V+AATRKQRF+RV+KDLKV RV STLVEEM+
Sbjct: 147 SFNWTQSSNLISQALSDESDQKVPKLKDFVMAATRKQRFERVTKDLKVKRVISTLVEEMR 206

Query: 212 KIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWLEADTNV 271
            IG   S  E  C+  + P +  +RSPVLLLMGGGMGAGKSTVLKDI  E FW EA  + 
Sbjct: 207 VIGSGSS--EPHCTEVMSPVAHNKRSPVLLLMGGGMGAGKSTVLKDIFLESFWSEAQADA 266

Query: 272 VNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGRDVILDS 331
           V +EADAFKETDVIY+A+SS G+HDDMLQTAELVHQ S DAASSLLVTALN+GRDVI+D 
Sbjct: 267 VVIEADAFKETDVIYRALSSRGHHDDMLQTAELVHQSSTDAASSLLVTALNDGRDVIMDG 326

Query: 332 TLSWEPYVMQTIEMARNIHNRRYRMGVGYKV-ENGNVTENYWEPVSEEEEEELRKEHGEM 391
           TLSWEP+V Q IEMARN+H ++YRMG GYKV E G +TE YW     EEEEE  KE+G+ 
Sbjct: 327 TLSWEPFVEQMIEMARNVHKQKYRMGEGYKVSEEGTITEKYW-----EEEEEETKENGKQ 386

Query: 392 QNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYSEVVDS 451
           QN +PYRIELVGVVCDA+LAV RGIRRA+M+ RAVRV  QL SHKRFANAFPKY E+VD+
Sbjct: 387 QNLKPYRIELVGVVCDAYLAVARGIRRALMVKRAVRVKPQLNSHKRFANAFPKYCELVDN 446

Query: 452 VRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPNPNPFS 511
            RLY TN++G PP+LI  KD +    +VDPE + CL  +S+LNPDAES+YELYP+P+  S
Sbjct: 447 ARLYCTNAVGGPPRLIAWKDGNSKL-LVDPEDIDCLKRVSSLNPDAESIYELYPDPSQLS 506

Query: 512 EPEPILKEIALTPSRVQSQKELRSAIQKLESSR 532
           +P  +  ++ L PSR + QKEL  AI+++E ++
Sbjct: 507 KPGSVWNDVVLVPSRPKVQKELSDAIRRIEKAQ 531

BLAST of Spg032113 vs. TAIR 10
Match: AT2G30630.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 543.1 bits (1398), Expect = 2.5e-154
Identity = 286/459 (62.31%), Postives = 357/459 (77.78%), Query Frame = 0

Query: 72  LERFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMD 131
           LERFSHYV RQ+G  D  E P LCKLAN Y+RK+  CEE+IY++F+ E  A+SL++KL++
Sbjct: 72  LERFSHYVVRQMGFKDRRECPHLCKLANEYIRKSGSCEEDIYSFFSEEPGADSLFIKLVE 131

Query: 132 EFDQCILSYFAFHWSQAPLMITQVLGVDSE-HKKLKDLVVAATRKQRFDRVSKDLKVTRV 191
           EF++CILSYFA+HWS A LMI+Q+L  D E  KKLK +V+AATR+QRF+RV+K+LKV RV
Sbjct: 132 EFERCILSYFAYHWSHADLMISQILSADVEPKKKLKHIVMAATREQRFERVTKNLKVARV 191

Query: 192 FSTLVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEP 251
           F+TLVEEMK +G A S  +S+C+  + P + ++RSPVLLLMGGGMGAGKSTVLKDI KEP
Sbjct: 192 FNTLVEEMKAMGIA-SVDDSECTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKEP 251

Query: 252 FWLEADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALN 311
           FW  AD   V +EADAFKE+DVIY+A+S+ G H DM++TAE VHQ S DAASSLLVTALN
Sbjct: 252 FWAGAD--AVVIEADAFKESDVIYRALSARG-HVDMIKTAEFVHQSSTDAASSLLVTALN 311

Query: 312 EGRDVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYKV-ENGNVTENYWEPVSEEEEE 371
           EGRDVI+D TLSW P+V+QTI MARN+H  RYRMG GYKV ENG+V ENYWE + E ++ 
Sbjct: 312 EGRDVIMDGTLSWVPFVVQTITMARNVHRHRYRMGAGYKVGENGDVIENYWERIGERQQ- 371

Query: 372 ELRKEHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAF 431
              +E G  + R+PYRIELVGVVCDA+LAV+RGIRRAIM  RAVRV SQL+SHKRFANAF
Sbjct: 372 --LQEDG--RERKPYRIELVGVVCDAYLAVIRGIRRAIMCRRAVRVRSQLRSHKRFANAF 431

Query: 432 PKYSEVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYE 491
             Y  +VD+ RLY TN++   PKLI  K+ +    +VDPE + CL  +  LN +A+S+YE
Sbjct: 432 LTYCNLVDNARLYCTNALEGSPKLIGWKEKEKTL-LVDPEEIDCLKNVGRLNENADSIYE 491

Query: 492 LYPNPNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLE 529
           LY NPNP  E   I K+I L+PSR   Q+EL+ +IQK+E
Sbjct: 492 LYRNPNPACEAGSIWKDIVLSPSRFNIQQELKYSIQKVE 520

BLAST of Spg032113 vs. TAIR 10
Match: AT2G30630.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 531.2 bits (1367), Expect = 9.8e-151
Identity = 287/480 (59.79%), Postives = 357/480 (74.38%), Query Frame = 0

Query: 72  LERFSHYVARQLGISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMD 131
           LERFSHYV RQ+G  D  E P LCKLAN Y+RK+  CEE+IY++F+ E  A+SL++KL++
Sbjct: 72  LERFSHYVVRQMGFKDRRECPHLCKLANEYIRKSGSCEEDIYSFFSEEPGADSLFIKLVE 131

Query: 132 EFDQCILSYFAFHWSQAPLMITQV---------------------LGVDSE-HKKLKDLV 191
           EF++CILSYFA+HWS A LMI+QV                     L  D E  KKLK +V
Sbjct: 132 EFERCILSYFAYHWSHADLMISQVTNKLSLSLLCYITNRPKTFQILSADVEPKKKLKHIV 191

Query: 192 VAATRKQRFDRVSKDLKVTRVFSTLVEEMKKIGCAPSKGESKCSHTIPPWSQRERSPVLL 251
           +AATR+QRF+RV+K+LKV RVF+TLVEEMK +G A S  +S+C+  + P + ++RSPVLL
Sbjct: 192 MAATREQRFERVTKNLKVARVFNTLVEEMKAMGIA-SVDDSECTEVMAPVAHKDRSPVLL 251

Query: 252 LMGGGMGAGKSTVLKDIRKEPFWLEADTNVVNVEADAFKETDVIYKAISSMGYHDDMLQT 311
           LMGGGMGAGKSTVLKDI KEPFW  AD   V +EADAFKE+DVIY+A+S+ G H DM++T
Sbjct: 252 LMGGGMGAGKSTVLKDILKEPFWAGAD--AVVIEADAFKESDVIYRALSARG-HVDMIKT 311

Query: 312 AELVHQPSIDAASSLLVTALNEGRDVILDSTLSWEPYVMQTIEMARNIHNRRYRMGVGYK 371
           AE VHQ S DAASSLLVTALNEGRDVI+D TLSW P+V+QTI MARN+H  RYRMG GYK
Sbjct: 312 AEFVHQSSTDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARNVHRHRYRMGAGYK 371

Query: 372 V-ENGNVTENYWEPVSEEEEEELRKEHGEMQNRRPYRIELVGVVCDAHLAVVRGIRRAIM 431
           V ENG+V ENYWE + E ++    +E G  + R+PYRIELVGVVCDA+LAV+RGIRRAIM
Sbjct: 372 VGENGDVIENYWERIGERQQ---LQEDG--RERKPYRIELVGVVCDAYLAVIRGIRRAIM 431

Query: 432 MGRAVRVNSQLQSHKRFANAFPKYSEVVDSVRLYSTNSIGNPPKLIHRKDNDDPFEMVDP 491
             RAVRV SQL+SHKRFANAF  Y  +VD+ RLY TN++   PKLI  K+ +    +VDP
Sbjct: 432 CRRAVRVRSQLRSHKRFANAFLTYCNLVDNARLYCTNALEGSPKLIGWKEKEKTL-LVDP 491

Query: 492 EALACLTALSNLNPDAESVYELYPNPNPFSEPEPILKEIALTPSRVQSQKELRSAIQKLE 529
           E + CL  +  LN +A+S+YELY NPNP  E   I K+I L+PSR   Q+EL+ +IQK+E
Sbjct: 492 EEIDCLKNVGRLNENADSIYELYRNPNPACEAGSIWKDIVLSPSRFNIQQELKYSIQKVE 541

BLAST of Spg032113 vs. TAIR 10
Match: AT1G06750.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 528.9 bits (1361), Expect = 4.9e-150
Identity = 291/507 (57.40%), Postives = 370/507 (72.98%), Query Frame = 0

Query: 24  SHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLERFSHYVARQL 83
           S  L  S +G + AA   +  R  +      +L   H H+      + LERFSHYVARQ+
Sbjct: 14  SQILVVSSIGLMIAAAVHYRLRRLRDTKIIPRLKSSHKHKGH----EKLERFSHYVARQM 73

Query: 84  GISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEFDQCILSYFAF 143
           G  D  E P LCKLA  Y+ K++ CEE+IY++F+ E +A++L++KL++EF++CILSYFAF
Sbjct: 74  GFKDRRECPNLCKLAAEYISKSECCEEDIYSFFSEEPDADTLFIKLVEEFERCILSYFAF 133

Query: 144 HWSQAPLMITQVLGVDSEHK-KLKDLVVAATRKQRFDRVSKDLKVTRVFSTLVEEMKKIG 203
           HWS A LMITQVL  D+E K KLK +V+AATR+ R  RV+K+LKV RVF+TLVEEM+ +G
Sbjct: 134 HWSHADLMITQVLSADAEPKRKLKQIVMAATRELRIKRVTKNLKVARVFNTLVEEMRAMG 193

Query: 204 CAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWLEADTNVVNV 263
            A S  +S+C+  + P + ++RSPVLLLMGGGMGAGKSTVLKDI KE FW  AD+  V +
Sbjct: 194 LA-SVDDSQCTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKEAFWAGADS--VVI 253

Query: 264 EADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGRDVILDSTLS 323
           EADAFKE+DVIY+A+SS G H DM+QTAELVHQ S DAASSLLVTALNEGRDVI+D TLS
Sbjct: 254 EADAFKESDVIYRALSSRG-HVDMIQTAELVHQSSTDAASSLLVTALNEGRDVIMDGTLS 313

Query: 324 WEPYVMQTIEMARNIHNRRYRMGVGYKV-ENGNVTENYWEPVSEEEEEELRKEHGEMQNR 383
           W P+V+QTI MARN+H   YRMG GYKV ++G +TENYWE + E ++ +   E G  + R
Sbjct: 314 WVPFVVQTITMARNVHRHHYRMGAGYKVGDDGVITENYWERIGERQQIQ---EDG--RRR 373

Query: 384 RPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYSEVVDSVRL 443
           +PYRIELVGVVCDA+LAV+RGIRRAIM  RAVRV SQL+SHKRFA AFP Y  +VD+ RL
Sbjct: 374 KPYRIELVGVVCDAYLAVIRGIRRAIMCRRAVRVRSQLRSHKRFAEAFPTYCSLVDNARL 433

Query: 444 YSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPNPNPFSEPE 503
           Y TN++   PKLI  K+ +    +VD E + CL  +  LN +A S+YELY  PNP  E  
Sbjct: 434 YCTNALEGSPKLIGWKEKEKTL-LVDTEEIDCLKRVGGLNENAGSIYELYRQPNPACEAG 493

Query: 504 PILKEIALTPSRVQSQKELRSAIQKLE 529
            I K+I L+PSR   Q+EL+ +IQK+E
Sbjct: 494 SIWKDIVLSPSRFSIQQELKYSIQKVE 506

BLAST of Spg032113 vs. TAIR 10
Match: AT1G06750.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 528.9 bits (1361), Expect = 4.9e-150
Identity = 291/507 (57.40%), Postives = 370/507 (72.98%), Query Frame = 0

Query: 24  SHYLSPSFVGGVAAAGKRHFRRTSKAVTNNHKLIPPHLHRTESGRVDFLERFSHYVARQL 83
           S  L  S +G + AA   +  R  +      +L   H H+      + LERFSHYVARQ+
Sbjct: 21  SQILVVSSIGLMIAAAVHYRLRRLRDTKIIPRLKSSHKHKGH----EKLERFSHYVARQM 80

Query: 84  GISDADEYPQLCKLANNYLRKTKGCEEEIYAYFASESEAESLYVKLMDEFDQCILSYFAF 143
           G  D  E P LCKLA  Y+ K++ CEE+IY++F+ E +A++L++KL++EF++CILSYFAF
Sbjct: 81  GFKDRRECPNLCKLAAEYISKSECCEEDIYSFFSEEPDADTLFIKLVEEFERCILSYFAF 140

Query: 144 HWSQAPLMITQVLGVDSEHK-KLKDLVVAATRKQRFDRVSKDLKVTRVFSTLVEEMKKIG 203
           HWS A LMITQVL  D+E K KLK +V+AATR+ R  RV+K+LKV RVF+TLVEEM+ +G
Sbjct: 141 HWSHADLMITQVLSADAEPKRKLKQIVMAATRELRIKRVTKNLKVARVFNTLVEEMRAMG 200

Query: 204 CAPSKGESKCSHTIPPWSQRERSPVLLLMGGGMGAGKSTVLKDIRKEPFWLEADTNVVNV 263
            A S  +S+C+  + P + ++RSPVLLLMGGGMGAGKSTVLKDI KE FW  AD+  V +
Sbjct: 201 LA-SVDDSQCTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKEAFWAGADS--VVI 260

Query: 264 EADAFKETDVIYKAISSMGYHDDMLQTAELVHQPSIDAASSLLVTALNEGRDVILDSTLS 323
           EADAFKE+DVIY+A+SS G H DM+QTAELVHQ S DAASSLLVTALNEGRDVI+D TLS
Sbjct: 261 EADAFKESDVIYRALSSRG-HVDMIQTAELVHQSSTDAASSLLVTALNEGRDVIMDGTLS 320

Query: 324 WEPYVMQTIEMARNIHNRRYRMGVGYKV-ENGNVTENYWEPVSEEEEEELRKEHGEMQNR 383
           W P+V+QTI MARN+H   YRMG GYKV ++G +TENYWE + E ++ +   E G  + R
Sbjct: 321 WVPFVVQTITMARNVHRHHYRMGAGYKVGDDGVITENYWERIGERQQIQ---EDG--RRR 380

Query: 384 RPYRIELVGVVCDAHLAVVRGIRRAIMMGRAVRVNSQLQSHKRFANAFPKYSEVVDSVRL 443
           +PYRIELVGVVCDA+LAV+RGIRRAIM  RAVRV SQL+SHKRFA AFP Y  +VD+ RL
Sbjct: 381 KPYRIELVGVVCDAYLAVIRGIRRAIMCRRAVRVRSQLRSHKRFAEAFPTYCSLVDNARL 440

Query: 444 YSTNSIGNPPKLIHRKDNDDPFEMVDPEALACLTALSNLNPDAESVYELYPNPNPFSEPE 503
           Y TN++   PKLI  K+ +    +VD E + CL  +  LN +A S+YELY  PNP  E  
Sbjct: 441 YCTNALEGSPKLIGWKEKEKTL-LVDTEEIDCLKRVGGLNENAGSIYELYRQPNPACEAG 500

Query: 504 PILKEIALTPSRVQSQKELRSAIQKLE 529
            I K+I L+PSR   Q+EL+ +IQK+E
Sbjct: 501 SIWKDIVLSPSRFSIQQELKYSIQKVE 513

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6582355.13.5e-26790.68Calmodulin calcium-dependent NAD kinase, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022924243.15.1e-26690.49uncharacterized protein LOC111431771 [Cucurbita moschata][more]
XP_023526441.13.3e-26590.11uncharacterized protein LOC111789938 [Cucurbita pepo subsp. pepo][more]
XP_022980774.13.3e-26590.11uncharacterized protein LOC111480063 [Cucurbita maxima][more]
XP_038877094.11.2e-26489.92uncharacterized protein LOC120069426 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q0WUY12.3e-16560.23Calmodulin calcium-dependent NAD kinase OS=Arabidopsis thaliana OX=3702 GN=NADKC... [more]
Match NameE-valueIdentityDescription
A0A6J1EBV02.4e-26690.49uncharacterized protein LOC111431771 OS=Cucurbita moschata OX=3662 GN=LOC1114317... [more]
A0A6J1J0661.6e-26590.11uncharacterized protein LOC111480063 OS=Cucurbita maxima OX=3661 GN=LOC111480063... [more]
A0A5A7U1T82.4e-26188.59P-loop nucleoside triphosphate hydrolase superfamily protein OS=Cucumis melo var... [more]
A0A0A0L4J63.1e-26188.59Zeta_toxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G143510 ... [more]
A0A1S3AXE19.0e-26188.40uncharacterized protein LOC103483643 OS=Cucumis melo OX=3656 GN=LOC103483643 PE=... [more]
Match NameE-valueIdentityDescription
AT1G04280.11.7e-16660.23P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G30630.12.5e-15462.31P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G30630.29.8e-15159.79P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G06750.14.9e-15057.40P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G06750.24.9e-15057.40P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 215..486
e-value: 1.1E-26
score: 95.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 215..358
IPR010488Zeta toxin domainPFAMPF06414Zeta_toxincoord: 221..335
e-value: 6.5E-14
score: 51.7
IPR044802Calmodulin calcium-dependent NAD kinase NADKc-likePANTHERPTHR31153UNCHARACTERIZEDcoord: 40..531

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg032113.1Spg032113.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016301 kinase activity