Spg032096 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg032096 vs. NCBI nr
Match: XP_004134140.1 (heat shock 70 kDa protein 8 [Cucumis sativus] >XP_011650982.1 heat shock 70 kDa protein 8 [Cucumis sativus] >KGN56991.1 hypothetical protein Csa_011014 [Cucumis sativus])

HSP 1 Score: 1072.4 bits (2772), Expect = 1.6e-309
Identity = 536/571 (93.87%), Postives = 564/571 (98.77%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGVSNELSHEYDMLSGA IFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 347
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQQQAVH++MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 348 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 407
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 408 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQD 467
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR++FEMVNSKVFEKCENLVKQCLHDAR+ I+D
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 468 VSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 527
           +SDVVVVGGCSYIPKIR LLMEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 528 DLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETK 587
           DLLSIQATPLAIGIRADG+SFI IIPKNTTMPARKELVFTT+QDNQSEALIVVYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 588 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVKM 647
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 648 PTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           PTVDDGHGWCAEALHGKYGA M+LVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Spg032096 vs. NCBI nr
Match: XP_038897373.1 (heat shock 70 kDa protein 8 [Benincasa hispida])

HSP 1 Score: 1068.5 bits (2762), Expect = 2.3e-308
Identity = 536/571 (93.87%), Postives = 560/571 (98.07%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGE+KTTSTFP+IAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEDKTTSTFPDIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGVSNELSHE+DMLSGA IFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEFDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKR I+NVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRSIKNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 347
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQQQAVHE+MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHESMGSGSEKIALIFNMGAGYCDVA 240

Query: 348 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 407
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 408 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQD 467
           AIHKLSFQS+VQIDVNLGNGSK+CKVLSR+EFEMVNSKVFEKCENLVKQCLHDARV I+D
Sbjct: 301 AIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVRIED 360

Query: 468 VSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 527
           +SDVVVVGGCSYIPKIR LLMEI K KALYEGMDPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRNLLMEICKNKALYEGMDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 528 DLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETK 587
           DLLSIQATPLAIGIRADG+SFI IIPKNTTMPARKELVFTTV+DNQSEALIVVYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVRDNQSEALIVVYEGEETR 480

Query: 588 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVKM 647
           AEENHLLGY KIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPG+QHPATP+MEVKM
Sbjct: 481 AEENHLLGYVKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGSQHPATPYMEVKM 540

Query: 648 PTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           PTVDDGHGWCAEALHGKYG  M+LV+LRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGPTMELVSLRKKS 571

BLAST of Spg032096 vs. NCBI nr
Match: KAA0049367.1 (heat shock 70 kDa protein 8 [Cucumis melo var. makuwa] >TYK17192.1 heat shock 70 kDa protein 8 [Cucumis melo var. makuwa])

HSP 1 Score: 1067.8 bits (2760), Expect = 4.0e-308
Identity = 535/571 (93.70%), Postives = 561/571 (98.25%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGS +ELLKNTR+QK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGVSNELSHEYDMLSGA IFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 347
           TRVERACAMAG QVRLMPEPTA+ALLYAQQQQQA H++MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 348 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 407
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 408 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQD 467
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR+EFEMVNSKVFEKCENLVKQCLHDARV I+D
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 468 VSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 527
           +SDVVVVGGCSYIPKIRKLLMEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 528 DLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETK 587
           DLLSIQATPLAIGIRADG+SFI IIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 588 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVKM 647
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 648 PTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           PTVDDGHGWCAEALHGKYGA M+LVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Spg032096 vs. NCBI nr
Match: XP_008438682.1 (PREDICTED: heat shock 70 kDa protein 8 [Cucumis melo])

HSP 1 Score: 1066.2 bits (2756), Expect = 1.1e-307
Identity = 534/571 (93.52%), Postives = 560/571 (98.07%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGS +ELLKNTR+QK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGVSNELSHEYDMLSGA IFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 347
           TRVERACAMAG QVRLMPEPTA+ALLYAQQQQQA H++MGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 348 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 407
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 408 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQD 467
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR+EFEMVNSKVFEKCENLVKQCLHDARV I+D
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 468 VSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 527
           +SDVVVVGGCSYIPKIRKLLMEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 528 DLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETK 587
           DLLSIQATPLAIGIRADG+SFI IIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 588 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVKM 647
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 648 PTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           PTVDDGHGWCAEALHGKYGA M+LVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Spg032096 vs. NCBI nr
Match: XP_022138266.1 (heat shock 70 kDa protein 8 [Momordica charantia])

HSP 1 Score: 1056.2 bits (2730), Expect = 1.2e-304
Identity = 531/571 (92.99%), Postives = 555/571 (97.20%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGVS+EL HEYDMLSGA IFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSSELCHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNN+WRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNMWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 347
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQ QAVHENMGSGSEKIAL+FNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQHQAVHENMGSGSEKIALVFNMGAGYCDVA 240

Query: 348 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 407
           +SAT GGVSQIRAL GSPIGGEDLLQNTMKYLLPNSESIFS+ GIDEIRRMGLLRVATQD
Sbjct: 241 ISATAGGVSQIRALTGSPIGGEDLLQNTMKYLLPNSESIFSNHGIDEIRRMGLLRVATQD 300

Query: 408 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQD 467
           AI KLSFQ++ QIDVNLGNGSKICKVLSR+EFEMVNSKVFEKCENLVKQCLHDARVEI+D
Sbjct: 301 AILKLSFQTSAQIDVNLGNGSKICKVLSREEFEMVNSKVFEKCENLVKQCLHDARVEIED 360

Query: 468 VSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 527
           +SDVVVVGGCS IPKIRKLLMEI+KKKALYE +DPLEAAVSGAA+EGAVASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSSIPKIRKLLMEISKKKALYEEIDPLEAAVSGAAIEGAVASGIGDPFGSL 420

Query: 528 DLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETK 587
           DLLSIQ TPLAIGIRADG++FI IIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEE +
Sbjct: 421 DLLSIQVTPLAIGIRADGNNFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEEAR 480

Query: 588 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVKM 647
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSN LRVLAGATLPGAQHPATPFMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNTLRVLAGATLPGAQHPATPFMEVKM 540

Query: 648 PTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           PT+DDGH WCAEALHGK+G +MDLVTLRKKS
Sbjct: 541 PTIDDGHSWCAEALHGKHGVSMDLVTLRKKS 571

BLAST of Spg032096 vs. ExPASy Swiss-Prot
Match: Q9SKY8 (Heat shock 70 kDa protein 8 OS=Arabidopsis thaliana OX=3702 GN=HSP70-8 PE=1 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 7.9e-243
Identity = 411/573 (71.73%), Postives = 492/573 (85.86%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTS--TFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQK 167
           MAE  YTVASDSE TGEEK++S  + PEIA+GIDIGTSQCSIA+WNGSQ+ +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 168 MMRSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQ 227
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GA IFNMKRL+GRVDTDPVVHASK+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 228 TLDVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRF 287
           TLD+GVRPF+AALVNN WRSTTPEEVLAIFLVEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 288 QLTRVERACAMAGLQV-RLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYC 347
           QLTR ERACAMAGL V RLMPEPTAIALLYAQQQQ   H+NMGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 348 DVAVSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 407
           DVAV+AT GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 408 TQDAIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVE 467
            QDAIH+L+ Q NVQI+V+LGNG+KI KVL R EFE VN KVFE+CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 468 IQDVSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPF 527
             D+ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGAV SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 528 GSLDLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGE 587
           GSLDLL+IQATPLA+G+RA+G+ FI +IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 588 ETKAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFME 647
               EENHLLGYFK++GIPPAPKGVPEI +CMDID+SN LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 648 VKMPTVDDGHGWCAEALHGKYGAAMDLVTLRKK 678
           V+MPTVDDGHGWCA+AL+ KYGA +DL+TL++K
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRK 562

BLAST of Spg032096 vs. ExPASy Swiss-Prot
Match: Q9LHA8 (Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.0e-76
Identity = 176/511 (34.44%), Postives = 286/511 (55.97%), Query Frame = 0

Query: 135 AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 194
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 195 GAVIFNMKRLIGRVDTDPVVHASKS-LPFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 254
              +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ +
Sbjct: 69  TNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISS 128

Query: 255 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 314
           + L++++ +AE  L  P++N V+T+P  F+  Q    + A  ++GL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAI 188

Query: 315 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 374
            Y       + +   S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AY------GLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 375 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVNLGNGSK 434
               + + +   +           R +  LR A + A   LS   Q+ ++ID +L  G  
Sbjct: 249 DNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLFEGID 308

Query: 435 ICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQDVSDVVVVGGCSYIPKIRKLLME 494
               ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++LL +
Sbjct: 309 FYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQD 368

Query: 495 ITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADGDSFI 554
               K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 555 SIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETKAEENHLLGYFKIIGIPPAPKGV 614
            +IP+NTT+P +KE +F+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPAP+GV
Sbjct: 429 VLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 615 PEITICMDIDSSNMLRVLAGATLPGAQHPAT 641
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Spg032096 vs. ExPASy Swiss-Prot
Match: P09189 (Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.7e-75
Identity = 179/515 (34.76%), Postives = 284/515 (55.15%), Query Frame = 0

Query: 135 AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 194
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVGFTDTERL--IGDAAKNQVAMNP 68

Query: 195 GAVIFNMKRLIGRVDTDPVVHAS-KSLPFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 254
              +F+ KRLIGR  +DP V +  K  PF V     G +P +        +    EE+ +
Sbjct: 69  INTVFDAKRLIGRRFSDPSVQSDIKLWPFKV-IPGPGDKPMIVVTYKGEEKQFAAEEISS 128

Query: 255 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 314
           + L ++K +AE  L   I+N V+T+P  F+  Q    + A  +AGL V R++ EPTA A+
Sbjct: 129 MVLTKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 315 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 374
            Y       + +   S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AY------GLDKKASSAGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 375 LQNTMKYLLPNSESIFSHRGIDEI----RRMGLLRVATQDAIHKLS--FQSNVQIDVNLG 434
               + + +      F  +   +I    R +  LR A + A   LS   Q+ ++ID +L 
Sbjct: 249 DNRMVNHFVQE----FKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLY 308

Query: 435 NGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQDVSDVVVVGGCSYIPKIRK 494
            G      ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++
Sbjct: 309 EGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQ 368

Query: 495 LLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADG 554
           LL +    K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G
Sbjct: 369 LLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAG 428

Query: 555 DSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETKAEENHLLGYFKIIGIPPA 614
                +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPA
Sbjct: 429 GGMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPA 488

Query: 615 PKGVPEITICMDIDSSNMLRVLAGATLPGAQHPAT 641
           P+GVP+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PRGVPQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Spg032096 vs. ExPASy Swiss-Prot
Match: Q9S9N1 (Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 6.3e-75
Identity = 178/517 (34.43%), Postives = 284/517 (54.93%), Query Frame = 0

Query: 129 STFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSH 188
           +T  E A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 189 EYDMLSGAVIFNMKRLIGRVDTDPVVHAS-KSLPFLVQTLDVGVRPFVAALVNNVWRSTT 248
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 249 PEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPE 308
           PEE+ ++ LV++K +AE  L R ++N V+T+P  F+  Q    + A A++GL V R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 309 PTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SP 368
           PTA A+ Y   ++           EK  LIF++G G  DV++     GV +++A AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 369 IGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVN 428
           +GGED     + + +               R +  LR A + A   LS   Q+ ++ID +
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEID-S 301

Query: 429 LGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQDVSDVVVVGGCSYIPKI 488
           L  G      +SR  FE +N  +F KC + V++ L DA+++   V DVV+VGG + IPKI
Sbjct: 302 LHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKI 361

Query: 489 RKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRA 548
           ++LL +    K L + ++P EA   GAA++ A+ +G G      DLL +   PL++G+  
Sbjct: 362 QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSE-KVQDLLLLDVAPLSLGLET 421

Query: 549 DGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETKAEENHLLGYFKIIGIP 608
            G     +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  +  +N+LLG F++ GIP
Sbjct: 422 AGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIP 481

Query: 609 PAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPAT 641
           PAP+GVP+I +C DID++ +L V A     G ++  T
Sbjct: 482 PAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Spg032096 vs. ExPASy Swiss-Prot
Match: P22953 (Heat shock 70 kDa protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP70-1 PE=1 SV=3)

HSP 1 Score: 281.2 bits (718), Expect = 3.1e-74
Identity = 174/511 (34.05%), Postives = 283/511 (55.38%), Query Frame = 0

Query: 135 AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 194
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 195 GAVIFNMKRLIGRVDTDPVVHASKSL-PFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 254
              +F+ KRLIGR  +D  V +   L PF +Q      +P +        +    EE+ +
Sbjct: 69  VNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQA-GPADKPMIYVEYKGEEKEFAAEEISS 128

Query: 255 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 314
           + L++++ +AE  L   I+N V+T+P  F+  Q    + A  +AGL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 315 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 374
            Y   ++        S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAT------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 375 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVNLGNGSK 434
               + + +   +           R +  LR + + A   LS   Q+ ++ID +L  G  
Sbjct: 249 DNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEID-SLYEGID 308

Query: 435 ICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQDVSDVVVVGGCSYIPKIRKLLME 494
               ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++LL +
Sbjct: 309 FYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQD 368

Query: 495 ITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADGDSFI 554
               K L + ++P EA   GAA++GA+ SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQGAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 555 SIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETKAEENHLLGYFKIIGIPPAPKGV 614
           ++IP+NTT+P +KE VF+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPAP+GV
Sbjct: 429 TLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 615 PEITICMDIDSSNMLRVLAGATLPGAQHPAT 641
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Spg032096 vs. ExPASy TrEMBL
Match: A0A0A0LAC6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G147740 PE=3 SV=1)

HSP 1 Score: 1072.4 bits (2772), Expect = 7.6e-310
Identity = 536/571 (93.87%), Postives = 564/571 (98.77%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGVSNELSHEYDMLSGA IFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFIVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 347
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQQQAVH++MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQAVHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 348 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 407
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 408 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQD 467
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR++FEMVNSKVFEKCENLVKQCLHDAR+ I+D
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREDFEMVNSKVFEKCENLVKQCLHDARLGIED 360

Query: 468 VSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 527
           +SDVVVVGGCSYIPKIR LLMEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRHLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 528 DLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETK 587
           DLLSIQATPLAIGIRADG+SFI IIPKNTTMPARKELVFTT+QDNQSEALIVVYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTIQDNQSEALIVVYEGEETR 480

Query: 588 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVKM 647
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPYMEVKM 540

Query: 648 PTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           PTVDDGHGWCAEALHGKYGA M+LVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Spg032096 vs. ExPASy TrEMBL
Match: A0A5D3D052 (Heat shock 70 kDa protein 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00370 PE=3 SV=1)

HSP 1 Score: 1067.8 bits (2760), Expect = 1.9e-308
Identity = 535/571 (93.70%), Postives = 561/571 (98.25%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGS +ELLKNTR+QK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGVSNELSHEYDMLSGA IFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 347
           TRVERACAMAG QVRLMPEPTA+ALLYAQQQQQA H++MGSGSEKIALIFNMGAGYCDVA
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDVA 240

Query: 348 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 407
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 408 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQD 467
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR+EFEMVNSKVFEKCENLVKQCLHDARV I+D
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 468 VSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 527
           +SDVVVVGGCSYIPKIRKLLMEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 528 DLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETK 587
           DLLSIQATPLAIGIRADG+SFI IIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 588 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVKM 647
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 648 PTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           PTVDDGHGWCAEALHGKYGA M+LVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Spg032096 vs. ExPASy TrEMBL
Match: A0A1S3AXP0 (heat shock 70 kDa protein 8 OS=Cucumis melo OX=3656 GN=LOC103483715 PE=3 SV=1)

HSP 1 Score: 1066.2 bits (2756), Expect = 5.5e-308
Identity = 534/571 (93.52%), Postives = 560/571 (98.07%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGE++TTSTFPEIAVGIDIGTSQCSIA+WNGS +ELLKNTR+QK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRTTSTFPEIAVGIDIGTSQCSIAVWNGSLVELLKNTRDQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGVSNELSHEYDMLSGA IFNMKRLIGRVDTDPVVHA KSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSNELSHEYDMLSGAAIFNMKRLIGRVDTDPVVHACKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNNVWRSTTPEEVLAIF+VELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNVWRSTTPEEVLAIFMVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 347
           TRVERACAMAG QVRLMPEPTA+ALLYAQQQQQA H++MGSGSEKIALIFNMGAGYCD A
Sbjct: 181 TRVERACAMAGFQVRLMPEPTAVALLYAQQQQQAAHDSMGSGSEKIALIFNMGAGYCDAA 240

Query: 348 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 407
           VSA GGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD
Sbjct: 241 VSAIGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 300

Query: 408 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQD 467
           AIHKLSFQS+V+I+V+LGNGSK+CKVLSR+EFEMVNSKVFEKCENLVKQCLHDARV I+D
Sbjct: 301 AIHKLSFQSSVEINVSLGNGSKLCKVLSREEFEMVNSKVFEKCENLVKQCLHDARVGIED 360

Query: 468 VSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 527
           +SDVVVVGGCSYIPKIRKLLMEI+KKKALYEG+DPLEAAVSGAALEGA+ASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSYIPKIRKLLMEISKKKALYEGIDPLEAAVSGAALEGAIASGIGDPFGSL 420

Query: 528 DLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETK 587
           DLLSIQATPLAIGIRADG+SFI IIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEET+
Sbjct: 421 DLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETR 480

Query: 588 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVKM 647
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGA LPGAQHPATP+MEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGAALPGAQHPATPYMEVKM 540

Query: 648 PTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           PTVDDGHGWCAEALHGKYGA M+LVTLRKKS
Sbjct: 541 PTVDDGHGWCAEALHGKYGATMELVTLRKKS 571

BLAST of Spg032096 vs. ExPASy TrEMBL
Match: A0A6J1C992 (heat shock 70 kDa protein 8 OS=Momordica charantia OX=3673 GN=LOC111009482 PE=3 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 5.7e-305
Identity = 531/571 (92.99%), Postives = 555/571 (97.20%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGVS+EL HEYDMLSGA IFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVSSELCHEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNN+WRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNMWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVA 347
           TRVERACAMAGLQVRLMPEPTA+ALLYAQQQ QAVHENMGSGSEKIAL+FNMGAGYCDVA
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQHQAVHENMGSGSEKIALVFNMGAGYCDVA 240

Query: 348 VSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQD 407
           +SAT GGVSQIRAL GSPIGGEDLLQNTMKYLLPNSESIFS+ GIDEIRRMGLLRVATQD
Sbjct: 241 ISATAGGVSQIRALTGSPIGGEDLLQNTMKYLLPNSESIFSNHGIDEIRRMGLLRVATQD 300

Query: 408 AIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQD 467
           AI KLSFQ++ QIDVNLGNGSKICKVLSR+EFEMVNSKVFEKCENLVKQCLHDARVEI+D
Sbjct: 301 AILKLSFQTSAQIDVNLGNGSKICKVLSREEFEMVNSKVFEKCENLVKQCLHDARVEIED 360

Query: 468 VSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSL 527
           +SDVVVVGGCS IPKIRKLLMEI+KKKALYE +DPLEAAVSGAA+EGAVASGIGDPFGSL
Sbjct: 361 LSDVVVVGGCSSIPKIRKLLMEISKKKALYEEIDPLEAAVSGAAIEGAVASGIGDPFGSL 420

Query: 528 DLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETK 587
           DLLSIQ TPLAIGIRADG++FI IIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEE +
Sbjct: 421 DLLSIQVTPLAIGIRADGNNFIPIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEEAR 480

Query: 588 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVKM 647
           AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSN LRVLAGATLPGAQHPATPFMEVKM
Sbjct: 481 AEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNTLRVLAGATLPGAQHPATPFMEVKM 540

Query: 648 PTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           PT+DDGH WCAEALHGK+G +MDLVTLRKKS
Sbjct: 541 PTIDDGHSWCAEALHGKHGVSMDLVTLRKKS 571

BLAST of Spg032096 vs. ExPASy TrEMBL
Match: A0A6J1GXD4 (heat shock 70 kDa protein 8 OS=Cucurbita moschata OX=3662 GN=LOC111457701 PE=3 SV=1)

HSP 1 Score: 1047.3 bits (2707), Expect = 2.7e-302
Identity = 530/572 (92.66%), Postives = 554/572 (96.85%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTSTFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMM 167
           MAEPIYTVASDSETTGE+++ S FPEIAVGIDIGTSQCSIA+WNGSQ+ELLKNTRNQK+M
Sbjct: 1   MAEPIYTVASDSETTGEDRSASIFPEIAVGIDIGTSQCSIAVWNGSQVELLKNTRNQKIM 60

Query: 168 RSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 227
           RSYVTFKDDTPSGGV NELS EYDMLSGA IFNMKRLIGRVDTDPVVHASKSLPFLVQTL
Sbjct: 61  RSYVTFKDDTPSGGVINELSQEYDMLSGAAIFNMKRLIGRVDTDPVVHASKSLPFLVQTL 120

Query: 228 DVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQL 287
           D+GVRPFVAALVNN+WRSTTPEEVLAIFLVELKMMAE+QLKRPIRNVVLTIPVSFSRFQL
Sbjct: 121 DIGVRPFVAALVNNIWRSTTPEEVLAIFLVELKMMAEHQLKRPIRNVVLTIPVSFSRFQL 180

Query: 288 TRVERACAMAGLQVRLMPEPTAIALLYA-QQQQQAVHENMGSGSEKIALIFNMGAGYCDV 347
           TRVERACAMAGLQVRLMPEPTA+ALLYA QQQQQAVHENMGSGSEKIALIFNMGAGYCDV
Sbjct: 181 TRVERACAMAGLQVRLMPEPTAVALLYAQQQQQQAVHENMGSGSEKIALIFNMGAGYCDV 240

Query: 348 AVSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQ 407
           AVSATGGGVSQI+ALAGSPIGGEDLLQNTMKYLLPN+ESIFSHRGIDEIRRMGLLRVATQ
Sbjct: 241 AVSATGGGVSQIKALAGSPIGGEDLLQNTMKYLLPNAESIFSHRGIDEIRRMGLLRVATQ 300

Query: 408 DAIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQ 467
           DAIHKLSFQS+VQIDVNLGNGSK+CKVLSR+EFEMVNS+VFEKCENLV QCLHDARV I 
Sbjct: 301 DAIHKLSFQSSVQIDVNLGNGSKLCKVLSREEFEMVNSEVFEKCENLVNQCLHDARVGID 360

Query: 468 DVSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGS 527
           D+SDVVVVGGCS IPKIR  LME +KKKALYEGM+PLEAAV GAALEGAVASGI DPFGS
Sbjct: 361 DLSDVVVVGGCSNIPKIRSFLMEKSKKKALYEGMNPLEAAVCGAALEGAVASGIADPFGS 420

Query: 528 LDLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEET 587
           LDLLSIQATPLAIGIRADG+SFI IIPKNTTMPARKELVFTTV+DNQSEALIVVYEGEET
Sbjct: 421 LDLLSIQATPLAIGIRADGNSFIPIIPKNTTMPARKELVFTTVKDNQSEALIVVYEGEET 480

Query: 588 KAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFMEVK 647
           +AEENHLLGYFKIIGIPPAPKG+PEITICMDIDSSNMLRVLAGA LPGAQHPATPFMEVK
Sbjct: 481 RAEENHLLGYFKIIGIPPAPKGIPEITICMDIDSSNMLRVLAGARLPGAQHPATPFMEVK 540

Query: 648 MPTVDDGHGWCAEALHGKYGAAMDLVTLRKKS 679
           MPTVDDG GWCAEALHGKYGAAMDLV+LRKKS
Sbjct: 541 MPTVDDGQGWCAEALHGKYGAAMDLVSLRKKS 572

BLAST of Spg032096 vs. TAIR 10
Match: AT2G32120.1 (heat-shock protein 70T-2 )

HSP 1 Score: 841.3 bits (2172), Expect = 5.6e-244
Identity = 411/573 (71.73%), Postives = 492/573 (85.86%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTS--TFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQK 167
           MAE  YTVASDSE TGEEK++S  + PEIA+GIDIGTSQCSIA+WNGSQ+ +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 168 MMRSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQ 227
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GA IFNMKRL+GRVDTDPVVHASK+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 228 TLDVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRF 287
           TLD+GVRPF+AALVNN WRSTTPEEVLAIFLVEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 288 QLTRVERACAMAGLQV-RLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYC 347
           QLTR ERACAMAGL V RLMPEPTAIALLYAQQQQ   H+NMGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 348 DVAVSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 407
           DVAV+AT GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 408 TQDAIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVE 467
            QDAIH+L+ Q NVQI+V+LGNG+KI KVL R EFE VN KVFE+CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 468 IQDVSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPF 527
             D+ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGAV SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 528 GSLDLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGE 587
           GSLDLL+IQATPLA+G+RA+G+ FI +IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 588 ETKAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFME 647
               EENHLLGYFK++GIPPAPKGVPEI +CMDID+SN LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 648 VKMPTVDDGHGWCAEALHGKYGAAMDLVTLRKK 678
           V+MPTVDDGHGWCA+AL+ KYGA +DL+TL++K
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRK 562

BLAST of Spg032096 vs. TAIR 10
Match: AT2G32120.2 (heat-shock protein 70T-2 )

HSP 1 Score: 841.3 bits (2172), Expect = 5.6e-244
Identity = 411/573 (71.73%), Postives = 492/573 (85.86%), Query Frame = 0

Query: 108 MAEPIYTVASDSETTGEEKTTS--TFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQK 167
           MAE  YTVASDSE TGEEK++S  + PEIA+GIDIGTSQCSIA+WNGSQ+ +L+NTRNQK
Sbjct: 1   MAEAAYTVASDSENTGEEKSSSSPSLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQK 60

Query: 168 MMRSYVTFKDDTPSGGVSNELSHEYDMLSGAVIFNMKRLIGRVDTDPVVHASKSLPFLVQ 227
           +++S+VTFKD+ P+GGVSN+L+HE +ML+GA IFNMKRL+GRVDTDPVVHASK+LPFLVQ
Sbjct: 61  LIKSFVTFKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHASKNLPFLVQ 120

Query: 228 TLDVGVRPFVAALVNNVWRSTTPEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRF 287
           TLD+GVRPF+AALVNN WRSTTPEEVLAIFLVEL++MAE QLKRP+RNVVLT+PVSFSRF
Sbjct: 121 TLDIGVRPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRF 180

Query: 288 QLTRVERACAMAGLQV-RLMPEPTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYC 347
           QLTR ERACAMAGL V RLMPEPTAIALLYAQQQQ   H+NMGSGSE++A+IFNMGAGYC
Sbjct: 181 QLTRFERACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYC 240

Query: 348 DVAVSATGGGVSQIRALAGSPIGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVA 407
           DVAV+AT GGVSQI+ALAGSPIGGED+LQNT++++ P +E              GLLRVA
Sbjct: 241 DVAVTATAGGVSQIKALAGSPIGGEDILQNTIRHIAPPNE-----------EASGLLRVA 300

Query: 408 TQDAIHKLSFQSNVQIDVNLGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVE 467
            QDAIH+L+ Q NVQI+V+LGNG+KI KVL R EFE VN KVFE+CE LV QCL DARV 
Sbjct: 301 AQDAIHRLTDQENVQIEVDLGNGNKISKVLDRLEFEEVNQKVFEECERLVVQCLRDARVN 360

Query: 468 IQDVSDVVVVGGCSYIPKIRKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPF 527
             D+ D+++VGGCSYIPK+R ++  + KK  +Y+G++PLEAAV GAALEGAV SGI DPF
Sbjct: 361 GGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHDPF 420

Query: 528 GSLDLLSIQATPLAIGIRADGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGE 587
           GSLDLL+IQATPLA+G+RA+G+ FI +IP+NT +PARK+L FTTVQDNQ EALI++YEGE
Sbjct: 421 GSLDLLTIQATPLAVGVRANGNKFIPVIPRNTMVPARKDLFFTTVQDNQKEALIIIYEGE 480

Query: 588 ETKAEENHLLGYFKIIGIPPAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPATPFME 647
               EENHLLGYFK++GIPPAPKGVPEI +CMDID+SN LRV A   +PG+  P  P +E
Sbjct: 481 GETVEENHLLGYFKLVGIPPAPKGVPEINVCMDIDASNALRVFAAVLMPGSSSPVVPVIE 540

Query: 648 VKMPTVDDGHGWCAEALHGKYGAAMDLVTLRKK 678
           V+MPTVDDGHGWCA+AL+ KYGA +DL+TL++K
Sbjct: 541 VRMPTVDDGHGWCAQALNVKYGATLDLITLQRK 562

BLAST of Spg032096 vs. TAIR 10
Match: AT3G12580.1 (heat shock protein 70 )

HSP 1 Score: 288.5 bits (737), Expect = 1.4e-77
Identity = 176/511 (34.44%), Postives = 286/511 (55.97%), Query Frame = 0

Query: 135 AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 194
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 195 GAVIFNMKRLIGRVDTDPVVHASKS-LPFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 254
              +F+ KRLIGR  +DP V A KS  PF V +   G +P +        +  + EE+ +
Sbjct: 69  TNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVS-GPGEKPMIVVNHKGEEKQFSAEEISS 128

Query: 255 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 314
           + L++++ +AE  L  P++N V+T+P  F+  Q    + A  ++GL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDAGVISGLNVMRIINEPTAAAI 188

Query: 315 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 374
            Y       + +   S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AY------GLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 375 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVNLGNGSK 434
               + + +   +           R +  LR A + A   LS   Q+ ++ID +L  G  
Sbjct: 249 DNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQTTIEID-SLFEGID 308

Query: 435 ICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQDVSDVVVVGGCSYIPKIRKLLME 494
               ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++LL +
Sbjct: 309 FYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGGSTRIPKVQQLLQD 368

Query: 495 ITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADGDSFI 554
               K L + ++P EA   GAA++ A+ SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQAAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 555 SIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETKAEENHLLGYFKIIGIPPAPKGV 614
            +IP+NTT+P +KE +F+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPAP+GV
Sbjct: 429 VLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 615 PEITICMDIDSSNMLRVLAGATLPGAQHPAT 641
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508

BLAST of Spg032096 vs. TAIR 10
Match: AT1G16030.1 (heat shock protein 70B )

HSP 1 Score: 283.5 bits (724), Expect = 4.5e-76
Identity = 178/517 (34.43%), Postives = 284/517 (54.93%), Query Frame = 0

Query: 129 STFPEIAVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSH 188
           +T  E A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   +
Sbjct: 2   ATKSEKAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVAFTDTERL--IGDAAKN 61

Query: 189 EYDMLSGAVIFNMKRLIGRVDTDPVVHAS-KSLPFLVQTLDVGVRPFVAALVNNVWRSTT 248
           +  +     +F+ KRLIGR  +DP V +     PF V +   G +P +     N  +  +
Sbjct: 62  QVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVS-GPGEKPMIVVSYKNEEKQFS 121

Query: 249 PEEVLAIFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPE 308
           PEE+ ++ LV++K +AE  L R ++N V+T+P  F+  Q    + A A++GL V R++ E
Sbjct: 122 PEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDAGAISGLNVLRIINE 181

Query: 309 PTAIALLYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SP 368
           PTA A+ Y   ++           EK  LIF++G G  DV++     GV +++A AG + 
Sbjct: 182 PTAAAIAYGLDKKGT------KAGEKNVLIFDLGGGTFDVSLLTIEEGVFEVKATAGDTH 241

Query: 369 IGGEDLLQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVN 428
           +GGED     + + +               R +  LR A + A   LS   Q+ ++ID +
Sbjct: 242 LGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQTTIEID-S 301

Query: 429 LGNGSKICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQDVSDVVVVGGCSYIPKI 488
           L  G      +SR  FE +N  +F KC + V++ L DA+++   V DVV+VGG + IPKI
Sbjct: 302 LHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGGSTRIPKI 361

Query: 489 RKLLMEITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRA 548
           ++LL +    K L + ++P EA   GAA++ A+ +G G      DLL +   PL++G+  
Sbjct: 362 QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSE-KVQDLLLLDVAPLSLGLET 421

Query: 549 DGDSFISIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETKAEENHLLGYFKIIGIP 608
            G     +IP+NTT+P +KE VF+T  DNQ   LI VYEGE  +  +N+LLG F++ GIP
Sbjct: 422 AGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTFELKGIP 481

Query: 609 PAPKGVPEITICMDIDSSNMLRVLAGATLPGAQHPAT 641
           PAP+GVP+I +C DID++ +L V A     G ++  T
Sbjct: 482 PAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQIT 507

BLAST of Spg032096 vs. TAIR 10
Match: AT5G02500.1 (heat shock cognate protein 70-1 )

HSP 1 Score: 281.2 bits (718), Expect = 2.2e-75
Identity = 174/511 (34.05%), Postives = 283/511 (55.38%), Query Frame = 0

Query: 135 AVGIDIGTSQCSIAIWNGSQIELLKNTRNQKMMRSYVTFKDDTPSGGVSNELSHEYDMLS 194
           A+GID+GT+   + +W   ++E++ N +  +   SYV F D      + +   ++  M  
Sbjct: 9   AIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVAFTDSERL--IGDAAKNQVAMNP 68

Query: 195 GAVIFNMKRLIGRVDTDPVVHASKSL-PFLVQTLDVGVRPFVAALVNNVWRSTTPEEVLA 254
              +F+ KRLIGR  +D  V +   L PF +Q      +P +        +    EE+ +
Sbjct: 69  VNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQA-GPADKPMIYVEYKGEEKEFAAEEISS 128

Query: 255 IFLVELKMMAEYQLKRPIRNVVLTIPVSFSRFQLTRVERACAMAGLQV-RLMPEPTAIAL 314
           + L++++ +AE  L   I+N V+T+P  F+  Q    + A  +AGL V R++ EPTA A+
Sbjct: 129 MVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDAGVIAGLNVMRIINEPTAAAI 188

Query: 315 LYAQQQQQAVHENMGSGSEKIALIFNMGAGYCDVAVSATGGGVSQIRALAG-SPIGGEDL 374
            Y   ++        S  EK  LIF++G G  DV++     G+ +++A AG + +GGED 
Sbjct: 189 AYGLDKKAT------SVGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDF 248

Query: 375 LQNTMKYLLPNSESIFSHRGIDEIRRMGLLRVATQDAIHKLS--FQSNVQIDVNLGNGSK 434
               + + +   +           R +  LR + + A   LS   Q+ ++ID +L  G  
Sbjct: 249 DNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQTTIEID-SLYEGID 308

Query: 435 ICKVLSRDEFEMVNSKVFEKCENLVKQCLHDARVEIQDVSDVVVVGGCSYIPKIRKLLME 494
               ++R  FE +N  +F KC   V++CL DA+++   V DVV+VGG + IPK+++LL +
Sbjct: 309 FYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGGSTRIPKVQQLLQD 368

Query: 495 ITKKKALYEGMDPLEAAVSGAALEGAVASGIGDPFGSLDLLSIQATPLAIGIRADGDSFI 554
               K L + ++P EA   GAA++GA+ SG G+     DLL +  TPL++G+   G    
Sbjct: 369 FFNGKELCKSINPDEAVAYGAAVQGAILSGEGNE-KVQDLLLLDVTPLSLGLETAGGVMT 428

Query: 555 SIIPKNTTMPARKELVFTTVQDNQSEALIVVYEGEETKAEENHLLGYFKIIGIPPAPKGV 614
           ++IP+NTT+P +KE VF+T  DNQ   LI VYEGE  + ++N+LLG F++ GIPPAP+GV
Sbjct: 429 TLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRGV 488

Query: 615 PEITICMDIDSSNMLRVLAGATLPGAQHPAT 641
           P+IT+C DID++ +L V A     G ++  T
Sbjct: 489 PQITVCFDIDANGILNVSAEDKTTGQKNKIT 508