Spg032048 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg032048
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionprotein disulfide isomerase pTAC5, chloroplastic
Locationscaffold11: 41442919 .. 41447321 (-)
RNA-Seq ExpressionSpg032048
SyntenySpg032048
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAGTTCTCACAGTCACTATCTTTTACTCCTTCGCCGTTCCTCCATTTCTCTCCCTTTCTTCCCCACTCTCTTCAATTTCTCTGCTACATTTCGATGTCTTCCTCGATCACTTTTCCTCTCAATCCTTCGCTTCATCTCAGACCTCTCCCCTTCTTCCCCTTCACTCTCTCAATCTTCTCATCTCCCAAGCTCTCATCTCGCTCTCTTGCCAGTTCCCTCATCTGTTGCTCTCTGAATCCCGCTAGTAACGATCGCGAGGAGCTCCGATGGCTTCGCGAGGAGCAGCGGTGGCTTCGCGAAGAGGAACGGTGGATCCGCGAAGAGCAGCGTTGGGCCAGAGAACGCGAGTCGCTTCTGCAGGAAATTGCTGAGCTTAAGCTGCAAATTCAGGCGTTAGAACGCCAGAATTCTATGCAAGGAGGGACGGTTTCGGTGTCGGAGACTATCGCGAATATTGCTGGTCTGTTGCAGGTGTTGAAGGAGAAGAATCTGATCGCCGAGAGCGGGGCGACGGTGAGTCGCATTTTGTTGGATGAGAGTACTCGCAAAGAGGACGTGGAGATAGAGAAGAAGGCGATTGTCGAAGAAGTGATAAAGAAGAAGGCAATTGTTGAAGAAGTTGTTAGGTTTTCCGATGAGATCAAGGCAGAGAAAGAGGTGAAGAACGAGAGAAAGTCTCTGAGAATTGGTTCGGAAGGAGCGGAAGTTCTAGCGATGCAGGTCTGCTCGCCATATGCATTTCTTCTATCAAATATTAGAAGGCGAGTTTTAAGTTCTGTGCTGCTTTTGTTCCTATGATTTTGTAGTCTTCGATAGATTTGGATCGAATTTTCTAGCCATATGCAAGCAATGTCGTAGATTAAACTGATTATGAACGTTATGAATTTGAGAATAGTTTTAAGATTAAAAAAAAGGATTAACTAGAATGAAGTTGAAAAAGATATGAATAGGAATAGATGTGATAGCAGTAGGCTTATGAATATAGTCGATTAAACTCTGTGGTAGATGCTGAGATATATGTTTGTTTGTGAAATTTAGTTTAGTCGATTTAGCTAGCTGAGATGCTTTAATTTCTTTTATTACGAAGATCATTGAAGAACATGTTTCTGGATGGTTTTGTCTCTACTAAAATGTCAGGAAGCATTGCTGAGGTTAGGATTTTACTCTGGTGAAGAAGACATGGAATTTTCAAGTTTCTCCAGTGGAACTGAGCGTGCTGTTAAGACTTGGCAAGTAAGATTATTCTGTAGTTGGCAGTAGTCTATCATTCTTTACCAATTCAATGGAATTTATATGGATTTAGTACACATTCTTTCAATCGATCATTAAAATATCTGTCGATTTGTAATATAGCTCTGAATTAGAGGATTCAACAAGGTATCTCCAATATTTTATATACATAGCCATAAAACATAAAAAAAAACACCCTCAATTTGTCACTGATATGAATATAAATACGAACAATAAGGTTCTAACTTATTTAGAAGCGAGAAAACTTAATTATTAATTTAAGTAATGTTCTTAATTTTTCTAATTTATTGGAATATCCATATATATCAATATCTTATCGTTATATCCGTAAAATAAAATTTTCAATATTGATATCGATATTTTAATCCTTGATTTCAATCAATGACACTTTATGGTGTACATAATTTTATTTGATAGGCTGCCTCGGGTTTCCGTGAAGATGGTATAATGACTGCAGAACTCCTTGAGATTTTATACAAGGAGAAAGTAACTGAGAGTGCTGGACCAGATGCCAAAACAGATGAAAAAGGGAATATTTCAACAGATCATAAAGTATGTTTGAAAACTTTTTATTTCATGAATCTAGTGCTGAATCAGAATCAGATGCCAGAGCTCCTTGGAGTTGTCTTTTTTTATGTCTTTCTGGTCGAAAGGAGTATCTGGTGTATCTATGGCTGCCTTTCTTGGATTGAAATATTTGGTTTTTTCTGATTTAAATGACGAATATATTTGATACTTGAGAGCCTACAGTCATCAAAATTCTTCCTATTTCCTTGAGTAGGCAATGTGTTGCTTAGGAACCTTCCAATTTTTTTAGTTTTTCTCAAGTAGTCCATGACATTCTCATGTGAATTTCTGTAGAGCTTAGAAGGTGCCATGCCTGCTCCATTGATATAAATAAAATTTCTCATCTCAATCTTCTCGAATTTCATTGAATAAAGAACCAATCTCATCTTTAGGGGTGCAAGCGAAAACTGCTCAAACCACCCAGTCCGACCGAAACCGCCAGCGGTTCGGTCCAGACGATCGGTTTCAGCTTGTGACTGTATGAAACCGAGGCTAATCAATTCGGCTTTGGTTCACCGTATAGGTGCGCCAACCGTGACCGAGCCAAATGATTATCCTAGAAAGACGAGTCGTCTTGCACAAGCAAATGGCAAGCTGTGTTAAAGCAAAAGATTGCAACGTTTTTAAGTATCACCTCTCTCGTTGATCATCTTTTGCGTATTGCTATTTGTCAGTTATGCTGTTTGCAACTTTTTGTCATTTTGTCTGTTTAAATGTTGGCAATTAAAGGTTTACTGTTTGATAAATGTTTGCATTCTGAAAACTGTTCTTCACGTTTTATAAATGTTTAGAAATGAGTGCTTTTCCCTCGTTCTCAGGAAGTTGACAATGGAGCTGTTGTTAATTCAATAACTGAAATATCAGAGATCCAGAAGACAGTGGTAAAAGAAGGCAGTGCAAGTTTTGATGTGTCCCAGCAGCGAGTTTTTCTCATAGGAGAGAACCGATGGGAAGACCCCGCACGACTTCATAGTTCAAATGGAAAAGCTTCTGATGGCAAAACTAAGGACACATCTACCAAGTGTCTGACTTGTCGTGGAGAAGGTCGTCTGTTATGCACAGGTACACGATCATCAATCATATATTTGAACTGCTATATATATATATATTAGTAACGTGGATTGAACTATCCAATGCTCTGATTCGTAGTCTTAAGAATGTCATGGGAGTGCTTTTCAATTTTCTAAAGAAACTTCGAGACCGAGCATCTTTGGAATTTTTGATGTTATAAAATGTCAATTTGACCAAAAGGTTAGATAGGGGTTGAACTAAAGAAAAAGTTACTTATAAAATAACTATGCCCCGTTTGATAACCATTTCATTTTTGGTTTTTGGTTTTTGAAATTTATGCTTGTTTTCTCCCAAATTTCCTACAATGGTTTTCATATTTGTTAAAGATCTATTTGAATTCCTAGCCAAATTCTAAAAACAAAAACAAGTTTTTGGAAACTACTTTTTTTTGTTTTCAAAATTTGGTTTGGTTTTTGAAAACATGGAAGAAGGTAGATACTAAGACAAAGAAACTTATGGAGGGAAATAGGTGTGTATAAGCTTATTTTTCAAAAACCAAAAACCAAAAACCAAATGGTTATCAAACGGGGCCTATATTATTGAAAAATACTATTTCTAACTTACCCAAAATGATTTTTTTTTTTTTTTTTTGTTAAATTACAAATTTAGTGTTTGAAGTTTGTGTCTATTTGTTCCCTAAACTTTCAAAATTATAAATTTAGTTCATGAACTTTTAAAATTCACAAATCTAGTAGACATACACAATTGAAACTTTAAGGTTCATTTGACATAAAATTCAAATTTATATCTAATTGATCAATTAATTTTTGAAATATTTTGAATTTATAAGGACATAAGATTGAAAGTTTAGGAACCAGATAGATAAACGTCAAAAGTTTATTAACTAAATTTGTAATTTAACCTTTTTTTTTTTTTTTTGTTTAAATCTTATTTTGGTCATTAGTATTTTCTTTTTGTTTCTTTCTTTTTCCTTTTTTTCTTACACCTTACACGTGTACATGCTGTTGTAAATGCTCTCTGTCTCGCCTGCATCTTATGAAATTATAAGCATTTGATAAACCACCAACAGCTCTACTCAGTACTCAGTATCTAACCAGTTTTACTTTCTCAGAATGCGATGGAACTGGTGAGCCAAATATTGAACCACAGGTGAAATCACTCGTACTCCCATTTTTTTTTTTTTTTAATTTCCTTCAAGTTTGGCTATTCTCTGGTAATTTTGTGTAAAAAGAATCAATTGAATGATGCAGTTTCTGGAATGGGTGGATGAAGGAACAAAATGTCCATATTGCGAAGGCCTTGGTTATACAATATGCGACGTATGTGAAGGGAAGACAGTAACATAGGTATGTATGACTACTCATCCATTGTAAATAATGGCTGTTTAATTGCTCACGTACTAAGATCCTATTCAATTTTGTTATATCTCATTTGAATATATATTGTTTACATACAGTAAAAATAAGATCATGGATAATCTTTTGTACCCTCTAGCATAGTATTGGTTCAACACGTGAGTGAGTGATTTGAACCTCCGACTTCTTGATCGAT

mRNA sequence

ATGTCTTCCTCGATCACTTTTCCTCTCAATCCTTCGCTTCATCTCAGACCTCTCCCCTTCTTCCCCTTCACTCTCTCAATCTTCTCATCTCCCAAGCTCTCATCTCGCTCTCTTGCCAGTTCCCTCATCTGTTGCTCTCTGAATCCCGCTAGTAACGATCGCGAGGAGCTCCGATGGCTTCGCGAGGAGCAGCGGTGGCTTCGCGAAGAGGAACGGTGGATCCGCGAAGAGCAGCGTTGGGCCAGAGAACGCGAGTCGCTTCTGCAGGAAATTGCTGAGCTTAAGCTGCAAATTCAGGCGTTAGAACGCCAGAATTCTATGCAAGGAGGGACGGTTTCGGTGTCGGAGACTATCGCGAATATTGCTGGTCTGTTGCAGGTGTTGAAGGAGAAGAATCTGATCGCCGAGAGCGGGGCGACGGTGAGTCGCATTTTGTTGGATGAGAGTACTCGCAAAGAGGACGTGGAGATAGAGAAGAAGGCGATTGTCGAAGAAGTGATAAAGAAGAAGGCAATTGTTGAAGAAGTTGTTAGGTTTTCCGATGAGATCAAGGCAGAGAAAGAGGTGAAGAACGAGAGAAAGTCTCTGAGAATTGGTTCGGAAGGAGCGGAAGTTCTAGCGATGCAGGAAGCATTGCTGAGGTTAGGATTTTACTCTGGTGAAGAAGACATGGAATTTTCAAGTTTCTCCAGTGGAACTGAGCGTGCTGTTAAGACTTGGCAAGCTGCCTCGGGTTTCCGTGAAGATGGTATAATGACTGCAGAACTCCTTGAGATTTTATACAAGGAGAAAGTAACTGAGAGTGCTGGACCAGATGCCAAAACAGATGAAAAAGGGAATATTTCAACAGATCATAAAAGCTTAGAAGGGGTGCAAGCGAAAACTGCTCAAACCACCCAGTCCGACCGAAACCGCCAGCGGTTCGTGCTTTTCCCTCGTTCTCAGGAAGTTGACAATGGAGCTGTTGTTAATTCAATAACTGAAATATCAGAGATCCAGAAGACAGTGGTAAAAGAAGGCAGTGCAAGTTTTGATGTGTCCCAGCAGCGAGTTTTTCTCATAGGAGAGAACCGATGGGAAGACCCCGCACGACTTCATAGTTCAAATGGAAAAGCTTCTGATGGCAAAACTAAGGACACATCTACCAAGTGTCTGACTTGTCGTGGAGAAGGTCGTCTGTTATGCACAGAATGCGATGGAACTGGTGAGCCAAATATTGAACCACAGTTTCTGGAATGGGTGGATGAAGGAACAAAATGTCCATATTGCGAAGGCCTTGGTTATACAATATGCGACGTATGTGAAGGGAAGACAGTAACATAG

Coding sequence (CDS)

ATGTCTTCCTCGATCACTTTTCCTCTCAATCCTTCGCTTCATCTCAGACCTCTCCCCTTCTTCCCCTTCACTCTCTCAATCTTCTCATCTCCCAAGCTCTCATCTCGCTCTCTTGCCAGTTCCCTCATCTGTTGCTCTCTGAATCCCGCTAGTAACGATCGCGAGGAGCTCCGATGGCTTCGCGAGGAGCAGCGGTGGCTTCGCGAAGAGGAACGGTGGATCCGCGAAGAGCAGCGTTGGGCCAGAGAACGCGAGTCGCTTCTGCAGGAAATTGCTGAGCTTAAGCTGCAAATTCAGGCGTTAGAACGCCAGAATTCTATGCAAGGAGGGACGGTTTCGGTGTCGGAGACTATCGCGAATATTGCTGGTCTGTTGCAGGTGTTGAAGGAGAAGAATCTGATCGCCGAGAGCGGGGCGACGGTGAGTCGCATTTTGTTGGATGAGAGTACTCGCAAAGAGGACGTGGAGATAGAGAAGAAGGCGATTGTCGAAGAAGTGATAAAGAAGAAGGCAATTGTTGAAGAAGTTGTTAGGTTTTCCGATGAGATCAAGGCAGAGAAAGAGGTGAAGAACGAGAGAAAGTCTCTGAGAATTGGTTCGGAAGGAGCGGAAGTTCTAGCGATGCAGGAAGCATTGCTGAGGTTAGGATTTTACTCTGGTGAAGAAGACATGGAATTTTCAAGTTTCTCCAGTGGAACTGAGCGTGCTGTTAAGACTTGGCAAGCTGCCTCGGGTTTCCGTGAAGATGGTATAATGACTGCAGAACTCCTTGAGATTTTATACAAGGAGAAAGTAACTGAGAGTGCTGGACCAGATGCCAAAACAGATGAAAAAGGGAATATTTCAACAGATCATAAAAGCTTAGAAGGGGTGCAAGCGAAAACTGCTCAAACCACCCAGTCCGACCGAAACCGCCAGCGGTTCGTGCTTTTCCCTCGTTCTCAGGAAGTTGACAATGGAGCTGTTGTTAATTCAATAACTGAAATATCAGAGATCCAGAAGACAGTGGTAAAAGAAGGCAGTGCAAGTTTTGATGTGTCCCAGCAGCGAGTTTTTCTCATAGGAGAGAACCGATGGGAAGACCCCGCACGACTTCATAGTTCAAATGGAAAAGCTTCTGATGGCAAAACTAAGGACACATCTACCAAGTGTCTGACTTGTCGTGGAGAAGGTCGTCTGTTATGCACAGAATGCGATGGAACTGGTGAGCCAAATATTGAACCACAGTTTCTGGAATGGGTGGATGAAGGAACAAAATGTCCATATTGCGAAGGCCTTGGTTATACAATATGCGACGTATGTGAAGGGAAGACAGTAACATAG

Protein sequence

MSSSITFPLNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSLNPASNDREELRWLREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIANIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRFSDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKTWQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTTQSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRWEDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTKCPYCEGLGYTICDVCEGKTVT
Homology
BLAST of Spg032048 vs. NCBI nr
Match: XP_022934883.1 (protein disulfide isomerase pTAC5, chloroplastic [Cucurbita moschata])

HSP 1 Score: 678.3 bits (1749), Expect = 4.4e-191
Identity = 367/440 (83.41%), Postives = 387/440 (87.95%), Query Frame = 0

Query: 1   MSSSITFP-LNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSLNPASNDREELRW 60
           MSSSIT P LNPSLHL P  FFP +LSIFSSPKLSSRS A+S IC SLNPASN+REE+RW
Sbjct: 1   MSSSITLPLLNPSLHLTPHRFFPVSLSIFSSPKLSSRSFANSFICRSLNPASNEREEVRW 60

Query: 61  LREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIA 120
           +REEQRW+REEERWIREEQRWARERESLLQEIAELKLQIQALERQ+SMQGGTVS SE+IA
Sbjct: 61  IREEQRWIREEERWIREEQRWARERESLLQEIAELKLQIQALERQHSMQGGTVSASESIA 120

Query: 121 NIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRF 180
           NIAGLLQVLKEKNLIAESGA+VSRILLDESTR+EDVEIEKK IVEEVI+ KAI+EEVVRF
Sbjct: 121 NIAGLLQVLKEKNLIAESGASVSRILLDESTREEDVEIEKKTIVEEVIENKAIIEEVVRF 180

Query: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240
           SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT
Sbjct: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240

Query: 241 WQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTT 300
           WQA SGFREDGIMTAELLEILY EK+TES G +AKTDEKGNI TD K  EG  A      
Sbjct: 241 WQATSGFREDGIMTAELLEILYGEKITESVGSNAKTDEKGNIPTDQK--EGANA------ 300

Query: 301 QSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRW 360
                                AVVNSITEISEI++TVVKEGSASFDVSQQRVFL+GENRW
Sbjct: 301 --------------------SAVVNSITEISEIERTVVKEGSASFDVSQQRVFLLGENRW 360

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDPARL SSNGKASDGKTKDTS KCLTCRGEGRLLC+ECDG+GEPN+EPQFLEWVDEG K
Sbjct: 361 EDPARLLSSNGKASDGKTKDTSPKCLTCRGEGRLLCSECDGSGEPNVEPQFLEWVDEGAK 412

Query: 421 CPYCEGLGYTICDVCEGKTV 440
           CPYCEGLGYTICD CEGKTV
Sbjct: 421 CPYCEGLGYTICDACEGKTV 412

BLAST of Spg032048 vs. NCBI nr
Match: XP_022974871.1 (protein disulfide isomerase pTAC5, chloroplastic [Cucurbita maxima])

HSP 1 Score: 676.0 bits (1743), Expect = 2.2e-190
Identity = 367/439 (83.60%), Postives = 384/439 (87.47%), Query Frame = 0

Query: 1   MSSSITFP-LNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSLNPASNDREELRW 60
           MSSSIT P LNPSLHL P  FFP +LSIFSSPKLSSRS A+S IC SLNPASN+REE+RW
Sbjct: 1   MSSSITLPLLNPSLHLTPHRFFPVSLSIFSSPKLSSRSFANSFICRSLNPASNEREEIRW 60

Query: 61  LREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIA 120
           +REEQRWLREEERWIREEQRW RER+SLLQEIAELKLQIQALERQ+SMQGGTVS SETIA
Sbjct: 61  IREEQRWLREEERWIREEQRWDRERKSLLQEIAELKLQIQALERQHSMQGGTVSASETIA 120

Query: 121 NIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRF 180
           NIAGLLQVLKEKNLIAESGA+VSRILLDESTR+EDVEIEKK IVEEVI+ KAIVE+VVR+
Sbjct: 121 NIAGLLQVLKEKNLIAESGASVSRILLDESTREEDVEIEKKTIVEEVIENKAIVEKVVRY 180

Query: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240
           SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT
Sbjct: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240

Query: 241 WQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTT 300
           WQA SGFREDGIMTAELLEILY EKVTES G DAKTDEKGNI TD K  + V        
Sbjct: 241 WQATSGFREDGIMTAELLEILYGEKVTESVGSDAKTDEKGNIPTDQKEGDNV-------- 300

Query: 301 QSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRW 360
                                AVVNSITEISEIQKTVVKEGSASFDVSQQRVFL+GENRW
Sbjct: 301 --------------------AAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLLGENRW 360

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDPARL SSNGKASDGKTK TSTKCLTCRGEGRLLC+ECDG+GEPN+EPQFLEWVDEG K
Sbjct: 361 EDPARLLSSNGKASDGKTKYTSTKCLTCRGEGRLLCSECDGSGEPNVEPQFLEWVDEGAK 411

Query: 421 CPYCEGLGYTICDVCEGKT 439
           CPYCEGLGYTICD CEGKT
Sbjct: 421 CPYCEGLGYTICDACEGKT 411

BLAST of Spg032048 vs. NCBI nr
Match: KAG6597298.1 (Protein disulfide isomerase pTAC5, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 674.1 bits (1738), Expect = 8.2e-190
Identity = 366/440 (83.18%), Postives = 383/440 (87.05%), Query Frame = 0

Query: 1   MSSSITFP-LNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSLNPASNDREELRW 60
           MSSSIT P LN SLHL P  FFP +LSIFSSPKLSSRS A+S IC SLNPASN+REE+RW
Sbjct: 1   MSSSITLPLLNLSLHLTPHRFFPVSLSIFSSPKLSSRSFANSFICRSLNPASNEREEIRW 60

Query: 61  LREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIA 120
           +REEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQ+SMQGGTVS SETIA
Sbjct: 61  IREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQHSMQGGTVSASETIA 120

Query: 121 NIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRF 180
           NIAGLLQVLKEKNLIAESGA+VSRI LDESTR+EDVEIEKK IVEEVI+ KAIVEEVVRF
Sbjct: 121 NIAGLLQVLKEKNLIAESGASVSRIFLDESTREEDVEIEKKTIVEEVIENKAIVEEVVRF 180

Query: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240
           SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT
Sbjct: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240

Query: 241 WQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTT 300
           WQA SGFREDGIMTAELLEILY EK+TES G DAKTDEKGNI TD K  + V        
Sbjct: 241 WQATSGFREDGIMTAELLEILYGEKITESVGSDAKTDEKGNIPTDQKEGDNV-------- 300

Query: 301 QSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRW 360
                                AVVNSITEISEI++TVVKEGSASFDVSQQRVFL+GENRW
Sbjct: 301 --------------------AAVVNSITEISEIERTVVKEGSASFDVSQQRVFLLGENRW 360

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDPARL SSNGKASDGKT  TSTKCLTCRGEGRLLC+ECDG+GEPN+EPQFLEWVDEG K
Sbjct: 361 EDPARLLSSNGKASDGKTNFTSTKCLTCRGEGRLLCSECDGSGEPNVEPQFLEWVDEGAK 412

Query: 421 CPYCEGLGYTICDVCEGKTV 440
           CPYCEGLGYTICD CEGKTV
Sbjct: 421 CPYCEGLGYTICDACEGKTV 412

BLAST of Spg032048 vs. NCBI nr
Match: KAG7028766.1 (hypothetical protein SDJN02_09947, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 674.1 bits (1738), Expect = 8.2e-190
Identity = 366/440 (83.18%), Postives = 383/440 (87.05%), Query Frame = 0

Query: 1   MSSSITFP-LNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSLNPASNDREELRW 60
           MSSSIT P LN SLHL P  FFP +LSIFSSPKLSSRS A+S IC SLNPASN+REE+RW
Sbjct: 32  MSSSITLPLLNLSLHLTPHRFFPVSLSIFSSPKLSSRSFANSFICRSLNPASNEREEIRW 91

Query: 61  LREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIA 120
           +REEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQ+SMQGGTVS SETIA
Sbjct: 92  IREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQHSMQGGTVSASETIA 151

Query: 121 NIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRF 180
           NIAGLLQVLKEKNLIAESGA+VSRI LDESTR+EDVEIEKK IVEEVI+ KAIVEEVVRF
Sbjct: 152 NIAGLLQVLKEKNLIAESGASVSRIFLDESTREEDVEIEKKTIVEEVIENKAIVEEVVRF 211

Query: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240
           SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT
Sbjct: 212 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 271

Query: 241 WQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTT 300
           WQA SGFREDGIMTAELLEILY EK+TES G DAKTDEKGNI TD K  + V        
Sbjct: 272 WQATSGFREDGIMTAELLEILYGEKITESVGSDAKTDEKGNIPTDQKEGDNV-------- 331

Query: 301 QSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRW 360
                                AVVNSITEISEI++TVVKEGSASFDVSQQRVFL+GENRW
Sbjct: 332 --------------------AAVVNSITEISEIERTVVKEGSASFDVSQQRVFLLGENRW 391

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDPARL SSNGKASDGKT  TSTKCLTCRGEGRLLC+ECDG+GEPN+EPQFLEWVDEG K
Sbjct: 392 EDPARLLSSNGKASDGKTNFTSTKCLTCRGEGRLLCSECDGSGEPNVEPQFLEWVDEGAK 443

Query: 421 CPYCEGLGYTICDVCEGKTV 440
           CPYCEGLGYTICD CEGKTV
Sbjct: 452 CPYCEGLGYTICDACEGKTV 443

BLAST of Spg032048 vs. NCBI nr
Match: XP_023538950.1 (protein disulfide isomerase pTAC5, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 668.7 bits (1724), Expect = 3.5e-188
Identity = 362/440 (82.27%), Postives = 380/440 (86.36%), Query Frame = 0

Query: 1   MSSSITFP-LNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSLNPASNDREELRW 60
           MSSSIT P LNPSLHL P   FP +LSIFSSPKLSSRS A+S IC SLNPASN+REE+RW
Sbjct: 1   MSSSITLPLLNPSLHLTPHRLFPVSLSIFSSPKLSSRSFANSFICRSLNPASNEREEIRW 60

Query: 61  LREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIA 120
           +REEQRWLREEERWIREEQRW RERESLLQEIAELKLQI+ LERQ+SMQGGTVS SETIA
Sbjct: 61  IREEQRWLREEERWIREEQRWTRERESLLQEIAELKLQIKVLERQHSMQGGTVSASETIA 120

Query: 121 NIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRF 180
           NIAGLLQVLKEKNLIAESGA+VSRILLDESTR+EDVEIEKK IVEEVI+ KAIVEEVVRF
Sbjct: 121 NIAGLLQVLKEKNLIAESGASVSRILLDESTREEDVEIEKKTIVEEVIENKAIVEEVVRF 180

Query: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240
           SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFS GTERAVKT
Sbjct: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSGGTERAVKT 240

Query: 241 WQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTT 300
           WQA SGF EDGIMTAELLEILY EKVTES G DAKTDEKGNI TD K  + V        
Sbjct: 241 WQATSGFHEDGIMTAELLEILYGEKVTESVGSDAKTDEKGNIPTDQKEGDNV-------- 300

Query: 301 QSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRW 360
                                AVVNSITEISEI++TVVKEGSASFDVSQQRVFL+GENRW
Sbjct: 301 --------------------AAVVNSITEISEIERTVVKEGSASFDVSQQRVFLLGENRW 360

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDPARL SSNGKASDGKT  TSTKCLTCRGEGRLLC+ECDG+GEPN+EPQFLEWVDEG K
Sbjct: 361 EDPARLLSSNGKASDGKTNFTSTKCLTCRGEGRLLCSECDGSGEPNVEPQFLEWVDEGAK 412

Query: 421 CPYCEGLGYTICDVCEGKTV 440
           CPYCEG+GYTICD CEGKTV
Sbjct: 421 CPYCEGIGYTICDACEGKTV 412

BLAST of Spg032048 vs. ExPASy Swiss-Prot
Match: A1A6M1 (Protein disulfide isomerase pTAC5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PTAC5 PE=1 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 3.4e-106
Identity = 230/437 (52.63%), Postives = 296/437 (67.73%), Query Frame = 0

Query: 3   SSITFPLNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSL-NPASNDREELRWLR 62
           +S + PL+    LR L     +L    SP     S+ SS++C S  NP   DREE+RWLR
Sbjct: 2   ASSSLPLSLPFPLRSLTSTTRSLPFQCSPLFF--SIPSSIVCFSTQNP---DREEVRWLR 61

Query: 63  EEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIANI 122
           EEQRW+REE+RWIREEQRW RERESLLQEI++L+L+IQ+LE +NS  G   S+ +TI+NI
Sbjct: 62  EEQRWIREEQRWIREEQRWIRERESLLQEISDLQLRIQSLESRNSQLGN--SIPDTISNI 121

Query: 123 AGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRFSD 182
           A LLQVLKEKN I+ESG + + ++L ESTR++ VE      VEE  K+  I EE VR S+
Sbjct: 122 AALLQVLKEKNRISESGLSATPMVL-ESTREQIVE-----EVEEEEKRVIIAEEKVRVSE 181

Query: 183 EIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKTWQ 242
            +K     K +R+ L++GSEG +V A+QEALL+LGFYSGEEDMEFSSFSSGT  AVKTWQ
Sbjct: 182 PVK-----KIKRRILKVGSEGDDVQALQEALLKLGFYSGEEDMEFSSFSSGTASAVKTWQ 241

Query: 243 AASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTTQS 302
           A+ G REDG+MTAELL+ L+ ++  E+   +A T +K                       
Sbjct: 242 ASLGVREDGVMTAELLQRLFMDEDVETDKDEASTMKK----------------------- 301

Query: 303 DRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGS-ASFDVSQQRVFLIGENRWE 362
                        +E  NGAV  S+T++ E ++++VK+ S    DV+Q RVFL+GENRWE
Sbjct: 302 -------------EEAGNGAVFTSVTQVPEKKQSIVKDQSDREVDVTQNRVFLLGENRWE 361

Query: 363 DPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTKC 422
           DP+RL   N      ++ +T T+C+TCRGEGRL+C ECDGTGEPNIEPQF+EWV E TKC
Sbjct: 362 DPSRLIGRNKPVDRSESTNTKTRCITCRGEGRLMCLECDGTGEPNIEPQFMEWVGEDTKC 384

Query: 423 PYCEGLGYTICDVCEGK 438
           PYCEGLGYT+CDVC+GK
Sbjct: 422 PYCEGLGYTVCDVCDGK 384

BLAST of Spg032048 vs. ExPASy TrEMBL
Match: A0A6J1F8Z4 (protein disulfide isomerase pTAC5, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111441890 PE=4 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 2.1e-191
Identity = 367/440 (83.41%), Postives = 387/440 (87.95%), Query Frame = 0

Query: 1   MSSSITFP-LNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSLNPASNDREELRW 60
           MSSSIT P LNPSLHL P  FFP +LSIFSSPKLSSRS A+S IC SLNPASN+REE+RW
Sbjct: 1   MSSSITLPLLNPSLHLTPHRFFPVSLSIFSSPKLSSRSFANSFICRSLNPASNEREEVRW 60

Query: 61  LREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIA 120
           +REEQRW+REEERWIREEQRWARERESLLQEIAELKLQIQALERQ+SMQGGTVS SE+IA
Sbjct: 61  IREEQRWIREEERWIREEQRWARERESLLQEIAELKLQIQALERQHSMQGGTVSASESIA 120

Query: 121 NIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRF 180
           NIAGLLQVLKEKNLIAESGA+VSRILLDESTR+EDVEIEKK IVEEVI+ KAI+EEVVRF
Sbjct: 121 NIAGLLQVLKEKNLIAESGASVSRILLDESTREEDVEIEKKTIVEEVIENKAIIEEVVRF 180

Query: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240
           SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT
Sbjct: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240

Query: 241 WQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTT 300
           WQA SGFREDGIMTAELLEILY EK+TES G +AKTDEKGNI TD K  EG  A      
Sbjct: 241 WQATSGFREDGIMTAELLEILYGEKITESVGSNAKTDEKGNIPTDQK--EGANA------ 300

Query: 301 QSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRW 360
                                AVVNSITEISEI++TVVKEGSASFDVSQQRVFL+GENRW
Sbjct: 301 --------------------SAVVNSITEISEIERTVVKEGSASFDVSQQRVFLLGENRW 360

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDPARL SSNGKASDGKTKDTS KCLTCRGEGRLLC+ECDG+GEPN+EPQFLEWVDEG K
Sbjct: 361 EDPARLLSSNGKASDGKTKDTSPKCLTCRGEGRLLCSECDGSGEPNVEPQFLEWVDEGAK 412

Query: 421 CPYCEGLGYTICDVCEGKTV 440
           CPYCEGLGYTICD CEGKTV
Sbjct: 421 CPYCEGLGYTICDACEGKTV 412

BLAST of Spg032048 vs. ExPASy TrEMBL
Match: A0A6J1ICM1 (protein disulfide isomerase pTAC5, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111473644 PE=4 SV=1)

HSP 1 Score: 676.0 bits (1743), Expect = 1.0e-190
Identity = 367/439 (83.60%), Postives = 384/439 (87.47%), Query Frame = 0

Query: 1   MSSSITFP-LNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSLNPASNDREELRW 60
           MSSSIT P LNPSLHL P  FFP +LSIFSSPKLSSRS A+S IC SLNPASN+REE+RW
Sbjct: 1   MSSSITLPLLNPSLHLTPHRFFPVSLSIFSSPKLSSRSFANSFICRSLNPASNEREEIRW 60

Query: 61  LREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIA 120
           +REEQRWLREEERWIREEQRW RER+SLLQEIAELKLQIQALERQ+SMQGGTVS SETIA
Sbjct: 61  IREEQRWLREEERWIREEQRWDRERKSLLQEIAELKLQIQALERQHSMQGGTVSASETIA 120

Query: 121 NIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRF 180
           NIAGLLQVLKEKNLIAESGA+VSRILLDESTR+EDVEIEKK IVEEVI+ KAIVE+VVR+
Sbjct: 121 NIAGLLQVLKEKNLIAESGASVSRILLDESTREEDVEIEKKTIVEEVIENKAIVEKVVRY 180

Query: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240
           SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT
Sbjct: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240

Query: 241 WQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTT 300
           WQA SGFREDGIMTAELLEILY EKVTES G DAKTDEKGNI TD K  + V        
Sbjct: 241 WQATSGFREDGIMTAELLEILYGEKVTESVGSDAKTDEKGNIPTDQKEGDNV-------- 300

Query: 301 QSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRW 360
                                AVVNSITEISEIQKTVVKEGSASFDVSQQRVFL+GENRW
Sbjct: 301 --------------------AAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLLGENRW 360

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDPARL SSNGKASDGKTK TSTKCLTCRGEGRLLC+ECDG+GEPN+EPQFLEWVDEG K
Sbjct: 361 EDPARLLSSNGKASDGKTKYTSTKCLTCRGEGRLLCSECDGSGEPNVEPQFLEWVDEGAK 411

Query: 421 CPYCEGLGYTICDVCEGKT 439
           CPYCEGLGYTICD CEGKT
Sbjct: 421 CPYCEGLGYTICDACEGKT 411

BLAST of Spg032048 vs. ExPASy TrEMBL
Match: A0A0A0L7Z4 (PG_binding_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G132000 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.6e-178
Identity = 346/441 (78.46%), Postives = 369/441 (83.67%), Query Frame = 0

Query: 1   MSSSITFPLNPSLHLRPLPFFPFTLSIFSSPKL-SSRSLASSLICCSLNPASNDREELRW 60
           MSSSIT PLNPSL L P     F+LS+FSSPKL SSRSL++SLIC SLNP+SNDREELRW
Sbjct: 1   MSSSITLPLNPSLLLTPR---AFSLSLFSSPKLSSSRSLSNSLICRSLNPSSNDREELRW 60

Query: 61  LREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIA 120
           +REEQRW REEERWIREEQRWARER+SLLQEIAELKLQIQALER+NS+QGGT+SVSETIA
Sbjct: 61  IREEQRWFREEERWIREEQRWARERQSLLQEIAELKLQIQALERRNSVQGGTISVSETIA 120

Query: 121 NIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRF 180
           NIAGLLQVLKEKNLIAESG TVSRILLDES+R+EDVEIEKK IVEEV+K          F
Sbjct: 121 NIAGLLQVLKEKNLIAESGPTVSRILLDESSREEDVEIEKKTIVEEVVK----------F 180

Query: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240
           S+E KAEKEVK ERKSLR GSEGAEVLAMQEAL+RLGFYSGEEDMEFSSFSSGTERAVKT
Sbjct: 181 SEESKAEKEVKKERKSLRTGSEGAEVLAMQEALMRLGFYSGEEDMEFSSFSSGTERAVKT 240

Query: 241 WQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTT 300
           WQAASGFREDGIMT ELL+IL+KE+VTES G DAKTDEKGNI TD +  E          
Sbjct: 241 WQAASGFREDGIMTTELLDILFKEEVTESVGSDAKTDEKGNIPTDQRGSE---------- 300

Query: 301 QSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRW 360
                              NG V+NS+TEI EIQKT+VKEGS SFDVSQQRVFLIGENRW
Sbjct: 301 -------------------NGTVINSVTEIQEIQKTIVKEGSESFDVSQQRVFLIGENRW 360

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDP RLHSSNGKASDGKTK  ST CLTCRGEGRLLCTECDGTGEPNIEPQFLEWV EGTK
Sbjct: 361 EDPTRLHSSNGKASDGKTKVISTNCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVGEGTK 399

Query: 421 CPYCEGLGYTICDVCEGKTVT 441
           CPYCEG+GY  CDVCEGKTVT
Sbjct: 421 CPYCEGVGYITCDVCEGKTVT 399

BLAST of Spg032048 vs. ExPASy TrEMBL
Match: A0A6J1CZV4 (uncharacterized protein LOC111016012 OS=Momordica charantia OX=3673 GN=LOC111016012 PE=4 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 8.6e-177
Identity = 343/441 (77.78%), Postives = 364/441 (82.54%), Query Frame = 0

Query: 1   MSSSITFPLNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSLNPASNDREELRWL 60
           MSSSIT PLNPSLHLR  P FPFTL IF SPK+SSRS A+S+ C SLNPA NDREE+RWL
Sbjct: 1   MSSSITLPLNPSLHLRSQPLFPFTLQIFPSPKISSRSFANSVACHSLNPAGNDREEVRWL 60

Query: 61  REEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIAN 120
           REEQRWLREEERWIREEQRW RERESLLQ IAELKLQIQALER+NS+QGG VSVSETIAN
Sbjct: 61  REEQRWLREEERWIREEQRWTRERESLLQGIAELKLQIQALERRNSIQGGAVSVSETIAN 120

Query: 121 IAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRFS 180
           IAGLLQ LK+KNLIAESG T S ILLDES+R+EDVEIE          KK IVEEV RFS
Sbjct: 121 IAGLLQALKDKNLIAESGPTASHILLDESSREEDVEIE----------KKEIVEEVARFS 180

Query: 181 DEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKTW 240
           D IKAEKEVK+ERKSLR GSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKTW
Sbjct: 181 DGIKAEKEVKSERKSLRNGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKTW 240

Query: 241 QAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTTQ 300
           QAASGF EDGIMTAELLEIL+K K+TES G DAKTD++GNI TD K              
Sbjct: 241 QAASGFPEDGIMTAELLEILFKGKLTESTGSDAKTDQQGNIPTDQK-------------- 300

Query: 301 SDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKE-GSASFDVSQQRVFLIGENRW 360
                          E +NGA+VNS TEISEIQKTVVKE G A+ ++SQQRVFLIGENRW
Sbjct: 301 ---------------EAENGAIVNSKTEISEIQKTVVKEGGGANLELSQQRVFLIGENRW 360

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDP+RL SSNGKASDGKTK    KC TCRGEGRLLCTECDG+GEPNIEPQFLEWVDEGTK
Sbjct: 361 EDPSRLRSSNGKASDGKTKGVLNKCRTCRGEGRLLCTECDGSGEPNIEPQFLEWVDEGTK 402

Query: 421 CPYCEGLGYTICDVCEGKTVT 441
           CPYCEGLGYTICDVCEGKTVT
Sbjct: 421 CPYCEGLGYTICDVCEGKTVT 402

BLAST of Spg032048 vs. ExPASy TrEMBL
Match: A0A1S3AWV2 (uncharacterized protein LOC103483490 OS=Cucumis melo OX=3656 GN=LOC103483490 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.8e-175
Identity = 341/440 (77.50%), Postives = 363/440 (82.50%), Query Frame = 0

Query: 1   MSSSITFPLNPSLHLRPLPFFPFTLSIFSSPKL-SSRSLASSLICCSLNPASNDREELRW 60
           MSSSIT PLNPSL L P      +LS+FSSPK  SSRSL++SLIC SLNPASNDREELRW
Sbjct: 1   MSSSITLPLNPSLLLTPR---ALSLSVFSSPKFSSSRSLSNSLICRSLNPASNDREELRW 60

Query: 61  LREEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIA 120
           +REEQRW REEERWIREEQRWARER+ LLQEIAELKLQIQALER+NS+QGGT+SVSETIA
Sbjct: 61  IREEQRWFREEERWIREEQRWARERQLLLQEIAELKLQIQALERRNSVQGGTISVSETIA 120

Query: 121 NIAGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRF 180
           NIAGLLQVLKEKNLIAESG TVSRILLDES+R+EDVEIEKK IVEEV+K          F
Sbjct: 121 NIAGLLQVLKEKNLIAESGPTVSRILLDESSREEDVEIEKKTIVEEVVK----------F 180

Query: 181 SDEIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKT 240
           S+E KAEKEVK ERKSLR+GSEGAEVLAMQEALL LGFY GEEDMEFSSFSSGTERAVKT
Sbjct: 181 SEESKAEKEVKKERKSLRVGSEGAEVLAMQEALLGLGFYCGEEDMEFSSFSSGTERAVKT 240

Query: 241 WQAASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTT 300
           WQ+ASGFREDGIMT ELLEIL+KEKVTES G DAKTDEKGNI TD +  E          
Sbjct: 241 WQSASGFREDGIMTTELLEILFKEKVTESVGSDAKTDEKGNIPTDQRGSE---------- 300

Query: 301 QSDRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGSASFDVSQQRVFLIGENRW 360
                              NG V+NSITEI EIQKT+VKEGSA  D+S+QRVFLIGENRW
Sbjct: 301 -------------------NGTVINSITEIQEIQKTIVKEGSAGLDLSEQRVFLIGENRW 360

Query: 361 EDPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTK 420
           EDP RLHSSNGKASDGKTK  ST CLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEG K
Sbjct: 361 EDPTRLHSSNGKASDGKTKVISTNCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGMK 398

Query: 421 CPYCEGLGYTICDVCEGKTV 440
           CPYCEG+GY  CDVCEGKTV
Sbjct: 421 CPYCEGVGYITCDVCEGKTV 398

BLAST of Spg032048 vs. TAIR 10
Match: AT4G13670.1 (plastid transcriptionally active 5 )

HSP 1 Score: 386.7 bits (992), Expect = 2.4e-107
Identity = 230/437 (52.63%), Postives = 296/437 (67.73%), Query Frame = 0

Query: 3   SSITFPLNPSLHLRPLPFFPFTLSIFSSPKLSSRSLASSLICCSL-NPASNDREELRWLR 62
           +S + PL+    LR L     +L    SP     S+ SS++C S  NP   DREE+RWLR
Sbjct: 2   ASSSLPLSLPFPLRSLTSTTRSLPFQCSPLFF--SIPSSIVCFSTQNP---DREEVRWLR 61

Query: 63  EEQRWLREEERWIREEQRWARERESLLQEIAELKLQIQALERQNSMQGGTVSVSETIANI 122
           EEQRW+REE+RWIREEQRW RERESLLQEI++L+L+IQ+LE +NS  G   S+ +TI+NI
Sbjct: 62  EEQRWIREEQRWIREEQRWIRERESLLQEISDLQLRIQSLESRNSQLGN--SIPDTISNI 121

Query: 123 AGLLQVLKEKNLIAESGATVSRILLDESTRKEDVEIEKKAIVEEVIKKKAIVEEVVRFSD 182
           A LLQVLKEKN I+ESG + + ++L ESTR++ VE      VEE  K+  I EE VR S+
Sbjct: 122 AALLQVLKEKNRISESGLSATPMVL-ESTREQIVE-----EVEEEEKRVIIAEEKVRVSE 181

Query: 183 EIKAEKEVKNERKSLRIGSEGAEVLAMQEALLRLGFYSGEEDMEFSSFSSGTERAVKTWQ 242
            +K     K +R+ L++GSEG +V A+QEALL+LGFYSGEEDMEFSSFSSGT  AVKTWQ
Sbjct: 182 PVK-----KIKRRILKVGSEGDDVQALQEALLKLGFYSGEEDMEFSSFSSGTASAVKTWQ 241

Query: 243 AASGFREDGIMTAELLEILYKEKVTESAGPDAKTDEKGNISTDHKSLEGVQAKTAQTTQS 302
           A+ G REDG+MTAELL+ L+ ++  E+   +A T +K                       
Sbjct: 242 ASLGVREDGVMTAELLQRLFMDEDVETDKDEASTMKK----------------------- 301

Query: 303 DRNRQRFVLFPRSQEVDNGAVVNSITEISEIQKTVVKEGS-ASFDVSQQRVFLIGENRWE 362
                        +E  NGAV  S+T++ E ++++VK+ S    DV+Q RVFL+GENRWE
Sbjct: 302 -------------EEAGNGAVFTSVTQVPEKKQSIVKDQSDREVDVTQNRVFLLGENRWE 361

Query: 363 DPARLHSSNGKASDGKTKDTSTKCLTCRGEGRLLCTECDGTGEPNIEPQFLEWVDEGTKC 422
           DP+RL   N      ++ +T T+C+TCRGEGRL+C ECDGTGEPNIEPQF+EWV E TKC
Sbjct: 362 DPSRLIGRNKPVDRSESTNTKTRCITCRGEGRLMCLECDGTGEPNIEPQFMEWVGEDTKC 384

Query: 423 PYCEGLGYTICDVCEGK 438
           PYCEGLGYT+CDVC+GK
Sbjct: 422 PYCEGLGYTVCDVCDGK 384

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022934883.14.4e-19183.41protein disulfide isomerase pTAC5, chloroplastic [Cucurbita moschata][more]
XP_022974871.12.2e-19083.60protein disulfide isomerase pTAC5, chloroplastic [Cucurbita maxima][more]
KAG6597298.18.2e-19083.18Protein disulfide isomerase pTAC5, chloroplastic, partial [Cucurbita argyrosperm... [more]
KAG7028766.18.2e-19083.18hypothetical protein SDJN02_09947, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023538950.13.5e-18882.27protein disulfide isomerase pTAC5, chloroplastic [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A1A6M13.4e-10652.63Protein disulfide isomerase pTAC5, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A6J1F8Z42.1e-19183.41protein disulfide isomerase pTAC5, chloroplastic OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1ICM11.0e-19083.60protein disulfide isomerase pTAC5, chloroplastic OS=Cucurbita maxima OX=3661 GN=... [more]
A0A0A0L7Z41.6e-17878.46PG_binding_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G13200... [more]
A0A6J1CZV48.6e-17777.78uncharacterized protein LOC111016012 OS=Momordica charantia OX=3673 GN=LOC111016... [more]
A0A1S3AWV22.8e-17577.50uncharacterized protein LOC103483490 OS=Cucumis melo OX=3656 GN=LOC103483490 PE=... [more]
Match NameE-valueIdentityDescription
AT4G13670.12.4e-10752.63plastid transcriptionally active 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 77..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..306
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 268..284
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 285..306
NoneNo IPR availablePANTHERPTHR15852:SF16PROTEIN DISULFIDE ISOMERASE PTAC5, CHLOROPLASTICcoord: 34..439
NoneNo IPR availablePANTHERPTHR15852PLASTID TRANSCRIPTIONALLY ACTIVE PROTEINcoord: 34..439
IPR036366PGBD superfamilyGENE3D1.10.101.10coord: 192..262
e-value: 2.0E-12
score: 48.8
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 202..260
e-value: 8.6E-11
score: 41.9
IPR036410Heat shock protein DnaJ, cysteine-rich domain superfamilySUPERFAMILY57938DnaJ/Hsp40 cysteine-rich domaincoord: 382..439
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 194..261

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg032048.1Spg032048.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0016020 membrane
molecular_function GO:0003756 protein disulfide isomerase activity