Spg031971 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031971
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionRING-type domain-containing protein
Locationscaffold11: 38703434 .. 38709050 (-)
RNA-Seq ExpressionSpg031971
SyntenySpg031971
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AATACATCAACATTTGGGAGTGAGGATTCAAACTTTTGACCTCATGGTCTACCTGTCAATTACCTTTGAGCTAAGCTTGCTTTGGCCAACTTTGTGTCAGTTGAGCTATGCTCTTGTTGGCATAGCTATCGAATTCTTCATTCTCATTCTATCACTCAATTTCATTAATATTATCGAACCGATTGAAAAAGAAAAATACCAAAACTCAAACACTTAGCATAAATAAAATTATTAAAAAAAAAAAGTGATAAAATGGGAGAGAGTAGTAGGCATCCTAACCATGGTAAATTAGAGAAGGCCCATTCAACTAACTCTAGTTACTTAATGTGGTTAACAAGAAAGCCAAAAAGTAAGAAAACAATACAATACAACAACAACGCCTGAGGCCAAAATATTCAGTAAAGAGAGAGAGAAACGCCATTATGCTCTACGCTACAGCAAGAAATTAAGAAACGAAAGGAAGAAAAAAAAAAAAAACAAAGAAAATAAACAAAGAAAAAAAAAAAGAAAAAAAAAAGAAAAGCTCTTATACGGATTTCTCTTTCTGATTCACCGATGTTCTTCTGATTTTGTATTTCCTTCCATCTTCGATTCTTCCAAATCACCAGCTTCAATCGGGTGCGTTTCTGCCGATCATTTCTCCGTTCTCTCTTCGACATTCAATTCTCGAATTTCTGTTTGGGGGCGGGGGATCTTCTTCTGTTCTTTTCGCAGTCCCTTTCCAATACAAATTTGTTGATAAAAGTTTTTGTTCCCTGGATCTACGAGGTTGGTTTTCGTATTCAATTCGCCGTGAAATTGGAATTAGATGATTGTGGTTTTCGCTCGTGTTCCACGGGTTTAACGTTTTCAGACGTCTGTAGATTCTTCTTCACTTTGTGTTTGTGTTTTCCTTTTTTGTTGTGATTGATTTGTTTTTCTCTTCATTTCCGGTGGACGCAGATTTTGATTATCGTCGATTTTAGTACTGAGATTCGAGGTATGCGTTTTAGTTCATTGTTTGATGAATACTCTAGATTCTCGATTCTTTAGTTCTTCGTCTTGTTTGTATCATCAGCTGCTGAATTTTGAGCTGTATACGATTCATTCATGCGATGCTAGTTGCTTTTTTCTCTTTCCTTGTCTTTCTTTTGGTTTGCATAAAATGGATGTTCGTGGTTTTCCAACTGCATTGAGGTATGAAAGACGTTTGGTACTTTTACTTGTTAAGTGTGTATTAATCCACACAAGAGAGAAGGAAAAAAGGATAACTAGATTGAGGTATTCATACATTGTTGTCTTGTGCAGTGGTCATCAATGGCATCAATGGTCGCTAAGCCCAGTTGTCCTAGTACTAGCAATCACGGGCCTTCTTCGATGACTGTCCAGGAAAAAGGGAGTAGGAATAAGAGGAAATACCGAGCGGATCCACCTTTAGGTGACCTGAATAAAATCACTTCTTCGTCTCAAGATGAATGTCCGAGTTATGAGTTTTCTGCTGAGAAATTTGAGATTAGTTCAAGTCTGGGGCAAACGAATGCGTGTGACCTTTGTAGCATTAGTCAAGAATTTTCTGCTGGATTGAAACTTGATCTCGGATTGTCCAATGGAGGCTCTTCTGATGTTGGGCTAAACTGGCCAAGAGGGGAATTAGAAGTTGATGAGTTCCAAGATGCCGATTGGAGTGACCTTACAGAAGCTCAGCTCGAAGAACTAGTTTTAAGCAATTTGGACACGATATTCAAGAGTGCCATTAAGAAAATTGTTGCTTCTGGATACACCGAAGAGGTTGCTATAAAAGCTGTCTCGAGGTCTGGCATCTGTTTTGGTTGTAAAGATACCGTGTCCAATATAGTGGACAACACCTTAGCTTTCCTTAGAAGTGGCCAAGAAATTGATCATTCAAGGGAACACTATTTTGAAGATTTACAGCAACTAGAAAAATATATTTTAGCTGAATTAGTTTGTGTACTACGGGAGGTTAGGCCTTTCTTCAGCACTGGTGATGCAATGTGGTGCTTATTGATTTGTGACATGAATGTATCTCATGCATGTGCAATGGATAGCGATCCATTTAATGCATTTGTCTGTGATGGGGCTTTGAACGAGAGTTCGTCTAACTCTATCCCACAGTTAAAAGCAGAAGCCAAAAGCTCTGAAATGAGTCTTCCTAAGCCTGTTAAGCCAGTTTCTCCAATCAGCTGTGCTCATAGTTCTCAATCTGATGGACCAGCCGCTCTAGGAGTTCCTAATCTTACAAAACCAAAGGAACCATTATTTTCAAGTGGACCAGTATCAGATAAAGAATTGCAAAATTCCACCTCTGATGTTGCTGAGGAATCATTTAGTGTGGCTGGAAACTCTCAAACTTCTGTGTCCGAAGAAAAAATTGGGAGCAGTAGAAAAGTTCATTCTAATATAACAAAGAGAGAATACATGCTACGACAAAAGTCGCTTCATGTGGATAAAAACTTTCGAACATATGGACCCAAGGGCTCATCAAGAGCTGGAAAGCTGACTGGTTTGGGGGGTTTAATGTTGGATAAGAAACTAAAGTCTGTTTCAGGCTCCACTGCAGTAAACTTTAAGAATGCTTCGCTGAAAATAAGCAAGGCTATGGGAATTGATGTGGCCCAAGACAATGGGAGTCATAATCTTTCCACCATCGACATTCCTTCTTCCTCTCTATCATTTAACTTGGAAAACATTAACACTGTTTCTCCTTTTTCTAAGACCAATGTACTATCTTCATTGCCCGCACCTAGTTCACCACCTGCATTACCTGCAACGAATATTTCTTCTGCGCTACCAGCAGCTGATATTGATCTTTCTCTTTCTTTGCCCACTAAAAGCAATCTGCCCTCAATGCCTATCAGCTGCAATGCTGAGTCTTCTACTAGTAGTTTTGTTGAGAAACCTTATGAAAAGTCCCTTGGGCAGTGGTTTCCTAGGGATAAGAAGGATGAGATGGTCTTGAAGCTAGTGCCAAGAGCTCGGGAATTACAAAATCAGCTGCAAGAGTGGACGGAATGGGCCAATCAAAAGGTCATGCAGGCTGCAAGGAGGCTAAGTAAGGACAAGGCTGAACTCAAAACATTGAAGCAAGAAAAGGAGGAAGTTGAACGGCTAAAAAAGGAGAAGCAGACTCTGGAGGAAAATACCATGAAAAAGCTTTCTGAGATGGAACACGCATTGTGCAAGGCTAGTGGGCAGGTTGAACTTGCTAACTCTGCTGTTAGGAGGCTTGAGGTGGAGAATGCTGCTCTACGGCAGGAGATGGAGGTTGCAAAATTACGTGCTACAGAATCAGCTGCTAGCTATCAAGAGGTTTCTAAGAGAGAGAAGAAAACACTGATGAAAGTTCAATCATGGGAGAAGCAAAAAATGTTGTTTCAGGAAGAACACACAGCCGAAAAACGAAAATTAAAACAACTAATACAGGAACTTGAGCAGGCCAGGGATCTCCAGGAGCAACTCGAGGTATGTTTTAACAAGTTACCCGTAGTCTCATTAGTTTTAGTTTCTATCTTTTAGTGCCTTCTTAATTATTAATTTTGGCAACTTGAAACTGTAGAACTGATGGAGTCGTAAGAAGAATGACAATAAATTGGGTGGTAAATCAAAATTTTATCACTTCCCACGAATAATTATGAGACACTAAATTATGGTTCTACCTGTAGATGGTTCAAAAGGCAATAACTGCCCTAGGAATGGAGTCCATGGTGGTGGTGTTAACAATGTGCATTCCATTTTATCTTTCCTAGGGGATTTCAACTGAACTTAAATCCTATTCTGCATAGATCATATTGCACTTTTTTGCTGTGCTTAGTTCCTGGTATGTTTGCAGATTACTGGTTGGTGTTTTCTCCCCCTTTAAAATATCCTACTATGCTCTCCTTTTTCCTTGTTGAAGCTGTTCTTTAAGTCGATTCTGTAGCTGTTGAGCATTACGAATTTTGTTTAAAAGATCACTCAGAGAAGTTACAAAAAGGTTTAGGGTCCATTTGGAAATACATTTTAATTTAAATGTTTTTCAAGAATGCATTCTTTAAAAAAACATTTTGTATAAAAATTGTTTAGAATTTAAACACTTAAATGTTTGGTTACATTTTCTCATAAATGTTTTTATACACAAGTTATTGTCTCTTTTTAAAATTTCTCAAAAAAAAAAAAAAAAAAAAACTCTTTTTAAAAAAGTTGGTTTTGAGTGTTTCTCTCAAAATGTTTTATTAAGTCATTTTTAGTGGTGGTCAAACACTAGAAATTTTTTCAAAATGATTTTTTTTTTCAAAATTAAACCCTTGAAAAGTTCAACCAAACACACCCTAAAATTGTTATCTTTGGATTCTTTCTTATAAAGCTGGATGTTGCATCTCATTTTGGGCGGAACATTAAGGTTCCGTTTGATAACCATTTGGTTTTTGAAAATTAAGCTTATACACACATACTTCCACCTATAAGTTTCTTGGTTTTAGTATCAATCTTCTACCTTGTTTTCAAAAACCAAGTCCAATTTTGGAAACAAAAAAAAGTAGATTTCAAAAATATGTTTTTGTTTTTAAAGTTTGGCTAGGAATTCAAATGCAACTTTAACTAGGATGAAAGCCATGGTAGGAAATCTAGAAGAAAACAAGTATAATTTTCAAAAACCAAAAACCAAAAACCAAATGGTTATCAAACGAGGCCTCAGATTTCCTTTTTTCCCCCTTGATATTAAATTTCAATTCTCATATGAATTTTCTTGCTTGGTGGGTCATGGTTTGCATCTTTTAATACATGATGCATTTACTTCTCCAACAATTAAATAAATTTTCTGAAAATTTGAGCATTGTATACTTTGTTTAAACCCAAAAAAGAAAAAAGAAAAAGAAAATCTGTATTTCCGTTTGTTTTATCTTGAACATACTTCCTTTGCGTTTTGTGATTGTGTTCCTAGTTTGTTTTTATATGTTCCTCACTGGTTTTCGACATACTTGTGATGGGCTTAAATATACCAATGCCCTACTCTAGCACTTAATTTGTACTGTTTCTCTTAGTTCTATTAATTACGTGGCGAATTGTGGGCCCTTTCTCATTGACAAGGAACATATCTCAGGGTAGATGGAAGTTAGAGGAGAGAGCAAAAGATGAGCTGCTCATGCAGGCTGCTTCATTGAGAAAGGAGAGAGAACAAATAGAAGCTTCAGTGAAAGCAAAGGAGGATACAATTAAATTGAAAGCAGAAAACAATCTTTTAAAATACAAAGACGATATCCAAAAGCTTGAAAAAGAAATCTCTCAGTTGAGACTTAAGACTGATTCTTCAAGAATTGCTGCTCTTAAGAGGGGCATCGACGGAAGTTATGCCAGTAGGCTTACAGATACCAGAAACAGCACAGATCACAAAGAGTCCTGGACCCCGAATGTCTCGGAATCGATGAAGGATCTTTACGAGTACTCTGGAACTGGAGGCGTGAAGCGGGAACGGGAGTGTGTGATGTGCCTTTCAGAGGAGATGTCAGTAGTTTTTCTTCCTTGCGCCCATCAGGTGGTGTGCACAACCTGCAATGAACTACATGAAAAACAGGGTATGAAAGATTGTCCTTCTTGTAGGAGCCCGATACAGCGGCGTATTCCAGTCCGTTATGCTCGCTCGTAA

mRNA sequence

ATGGCATCAATGGTCGCTAAGCCCAGTTGTCCTAGTACTAGCAATCACGGGCCTTCTTCGATGACTGTCCAGGAAAAAGGGAGTAGGAATAAGAGGAAATACCGAGCGGATCCACCTTTAGGTGACCTGAATAAAATCACTTCTTCGTCTCAAGATGAATGTCCGAGTTATGAGTTTTCTGCTGAGAAATTTGAGATTAGTTCAAGTCTGGGGCAAACGAATGCGTGTGACCTTTGTAGCATTAGTCAAGAATTTTCTGCTGGATTGAAACTTGATCTCGGATTGTCCAATGGAGGCTCTTCTGATGTTGGGCTAAACTGGCCAAGAGGGGAATTAGAAGTTGATGAGTTCCAAGATGCCGATTGGAGTGACCTTACAGAAGCTCAGCTCGAAGAACTAGTTTTAAGCAATTTGGACACGATATTCAAGAGTGCCATTAAGAAAATTGTTGCTTCTGGATACACCGAAGAGGTTGCTATAAAAGCTGTCTCGAGGTCTGGCATCTGTTTTGGTTGTAAAGATACCGTGTCCAATATAGTGGACAACACCTTAGCTTTCCTTAGAAGTGGCCAAGAAATTGATCATTCAAGGGAACACTATTTTGAAGATTTACAGCAACTAGAAAAATATATTTTAGCTGAATTAGTTTGTGTACTACGGGAGGTTAGGCCTTTCTTCAGCACTGGTGATGCAATGTGGTGCTTATTGATTTGTGACATGAATGTATCTCATGCATGTGCAATGGATAGCGATCCATTTAATGCATTTGTCTGTGATGGGGCTTTGAACGAGAGTTCGTCTAACTCTATCCCACAGTTAAAAGCAGAAGCCAAAAGCTCTGAAATGAGTCTTCCTAAGCCTGTTAAGCCAGTTTCTCCAATCAGCTGTGCTCATAGTTCTCAATCTGATGGACCAGCCGCTCTAGGAGTTCCTAATCTTACAAAACCAAAGGAACCATTATTTTCAAGTGGACCAGTATCAGATAAAGAATTGCAAAATTCCACCTCTGATGTTGCTGAGGAATCATTTAGTGTGGCTGGAAACTCTCAAACTTCTGTGTCCGAAGAAAAAATTGGGAGCAGTAGAAAAGTTCATTCTAATATAACAAAGAGAGAATACATGCTACGACAAAAGTCGCTTCATGTGGATAAAAACTTTCGAACATATGGACCCAAGGGCTCATCAAGAGCTGGAAAGCTGACTGGTTTGGGGGGTTTAATGTTGGATAAGAAACTAAAGTCTGTTTCAGGCTCCACTGCAGTAAACTTTAAGAATGCTTCGCTGAAAATAAGCAAGGCTATGGGAATTGATGTGGCCCAAGACAATGGGAGTCATAATCTTTCCACCATCGACATTCCTTCTTCCTCTCTATCATTTAACTTGGAAAACATTAACACTGTTTCTCCTTTTTCTAAGACCAATGTACTATCTTCATTGCCCGCACCTAGTTCACCACCTGCATTACCTGCAACGAATATTTCTTCTGCGCTACCAGCAGCTGATATTGATCTTTCTCTTTCTTTGCCCACTAAAAGCAATCTGCCCTCAATGCCTATCAGCTGCAATGCTGAGTCTTCTACTAGTAGTTTTGTTGAGAAACCTTATGAAAAGTCCCTTGGGCAGTGGTTTCCTAGGGATAAGAAGGATGAGATGGTCTTGAAGCTAGTGCCAAGAGCTCGGGAATTACAAAATCAGCTGCAAGAGTGGACGGAATGGGCCAATCAAAAGGTCATGCAGGCTGCAAGGAGGCTAAGTAAGGACAAGGCTGAACTCAAAACATTGAAGCAAGAAAAGGAGGAAGTTGAACGGCTAAAAAAGGAGAAGCAGACTCTGGAGGAAAATACCATGAAAAAGCTTTCTGAGATGGAACACGCATTGTGCAAGGCTAGTGGGCAGGTTGAACTTGCTAACTCTGCTGTTAGGAGGCTTGAGGTGGAGAATGCTGCTCTACGGCAGGAGATGGAGGTTGCAAAATTACGTGCTACAGAATCAGCTGCTAGCTATCAAGAGGTTTCTAAGAGAGAGAAGAAAACACTGATGAAAGTTCAATCATGGGAGAAGCAAAAAATGTTGTTTCAGGAAGAACACACAGCCGAAAAACGAAAATTAAAACAACTAATACAGGAACTTGAGCAGGCCAGGGATCTCCAGGAGCAACTCGAGATGGTTCAAAAGGCAATAACTGCCCTAGGAATGGAGTCCATGGTGGTGGTATCATATTGCACTTTTTTGCTGTGCTTAGTTCCTGTCGATTCTGTAGCTGTTGAGCATTACGAATTTTGTTTAAAAGATCACTCAGAGAAGTTACAAAAAGTTCTATTAATTACGTGGCGAATTGTGGGCCCTTTCTCATTGACAAGGAACATATCTCAGGGTAGATGGAAGTTAGAGGAGAGAGCAAAAGATGAGCTGCTCATGCAGGCTGCTTCATTGAGAAAGGAGAGAGAACAAATAGAAGCTTCAGTGAAAGCAAAGGAGGATACAATTAAATTGAAAGCAGAAAACAATCTTTTAAAATACAAAGACGATATCCAAAAGCTTGAAAAAGAAATCTCTCAGTTGAGACTTAAGACTGATTCTTCAAGAATTGCTGCTCTTAAGAGGGGCATCGACGGAAGTTATGCCAGTAGGCTTACAGATACCAGAAACAGCACAGATCACAAAGAGTCCTGGACCCCGAATGTCTCGGAATCGATGAAGGATCTTTACGAGTACTCTGGAACTGGAGGCGTGAAGCGGGAACGGGAGTGTGTGATGTGCCTTTCAGAGGAGATGTCAGTAGTTTTTCTTCCTTGCGCCCATCAGGTGGTGTGCACAACCTGCAATGAACTACATGAAAAACAGGGTATGAAAGATTGTCCTTCTTGTAGGAGCCCGATACAGCGGCGTATTCCAGTCCGTTATGCTCGCTCGTAA

Coding sequence (CDS)

ATGGCATCAATGGTCGCTAAGCCCAGTTGTCCTAGTACTAGCAATCACGGGCCTTCTTCGATGACTGTCCAGGAAAAAGGGAGTAGGAATAAGAGGAAATACCGAGCGGATCCACCTTTAGGTGACCTGAATAAAATCACTTCTTCGTCTCAAGATGAATGTCCGAGTTATGAGTTTTCTGCTGAGAAATTTGAGATTAGTTCAAGTCTGGGGCAAACGAATGCGTGTGACCTTTGTAGCATTAGTCAAGAATTTTCTGCTGGATTGAAACTTGATCTCGGATTGTCCAATGGAGGCTCTTCTGATGTTGGGCTAAACTGGCCAAGAGGGGAATTAGAAGTTGATGAGTTCCAAGATGCCGATTGGAGTGACCTTACAGAAGCTCAGCTCGAAGAACTAGTTTTAAGCAATTTGGACACGATATTCAAGAGTGCCATTAAGAAAATTGTTGCTTCTGGATACACCGAAGAGGTTGCTATAAAAGCTGTCTCGAGGTCTGGCATCTGTTTTGGTTGTAAAGATACCGTGTCCAATATAGTGGACAACACCTTAGCTTTCCTTAGAAGTGGCCAAGAAATTGATCATTCAAGGGAACACTATTTTGAAGATTTACAGCAACTAGAAAAATATATTTTAGCTGAATTAGTTTGTGTACTACGGGAGGTTAGGCCTTTCTTCAGCACTGGTGATGCAATGTGGTGCTTATTGATTTGTGACATGAATGTATCTCATGCATGTGCAATGGATAGCGATCCATTTAATGCATTTGTCTGTGATGGGGCTTTGAACGAGAGTTCGTCTAACTCTATCCCACAGTTAAAAGCAGAAGCCAAAAGCTCTGAAATGAGTCTTCCTAAGCCTGTTAAGCCAGTTTCTCCAATCAGCTGTGCTCATAGTTCTCAATCTGATGGACCAGCCGCTCTAGGAGTTCCTAATCTTACAAAACCAAAGGAACCATTATTTTCAAGTGGACCAGTATCAGATAAAGAATTGCAAAATTCCACCTCTGATGTTGCTGAGGAATCATTTAGTGTGGCTGGAAACTCTCAAACTTCTGTGTCCGAAGAAAAAATTGGGAGCAGTAGAAAAGTTCATTCTAATATAACAAAGAGAGAATACATGCTACGACAAAAGTCGCTTCATGTGGATAAAAACTTTCGAACATATGGACCCAAGGGCTCATCAAGAGCTGGAAAGCTGACTGGTTTGGGGGGTTTAATGTTGGATAAGAAACTAAAGTCTGTTTCAGGCTCCACTGCAGTAAACTTTAAGAATGCTTCGCTGAAAATAAGCAAGGCTATGGGAATTGATGTGGCCCAAGACAATGGGAGTCATAATCTTTCCACCATCGACATTCCTTCTTCCTCTCTATCATTTAACTTGGAAAACATTAACACTGTTTCTCCTTTTTCTAAGACCAATGTACTATCTTCATTGCCCGCACCTAGTTCACCACCTGCATTACCTGCAACGAATATTTCTTCTGCGCTACCAGCAGCTGATATTGATCTTTCTCTTTCTTTGCCCACTAAAAGCAATCTGCCCTCAATGCCTATCAGCTGCAATGCTGAGTCTTCTACTAGTAGTTTTGTTGAGAAACCTTATGAAAAGTCCCTTGGGCAGTGGTTTCCTAGGGATAAGAAGGATGAGATGGTCTTGAAGCTAGTGCCAAGAGCTCGGGAATTACAAAATCAGCTGCAAGAGTGGACGGAATGGGCCAATCAAAAGGTCATGCAGGCTGCAAGGAGGCTAAGTAAGGACAAGGCTGAACTCAAAACATTGAAGCAAGAAAAGGAGGAAGTTGAACGGCTAAAAAAGGAGAAGCAGACTCTGGAGGAAAATACCATGAAAAAGCTTTCTGAGATGGAACACGCATTGTGCAAGGCTAGTGGGCAGGTTGAACTTGCTAACTCTGCTGTTAGGAGGCTTGAGGTGGAGAATGCTGCTCTACGGCAGGAGATGGAGGTTGCAAAATTACGTGCTACAGAATCAGCTGCTAGCTATCAAGAGGTTTCTAAGAGAGAGAAGAAAACACTGATGAAAGTTCAATCATGGGAGAAGCAAAAAATGTTGTTTCAGGAAGAACACACAGCCGAAAAACGAAAATTAAAACAACTAATACAGGAACTTGAGCAGGCCAGGGATCTCCAGGAGCAACTCGAGATGGTTCAAAAGGCAATAACTGCCCTAGGAATGGAGTCCATGGTGGTGGTATCATATTGCACTTTTTTGCTGTGCTTAGTTCCTGTCGATTCTGTAGCTGTTGAGCATTACGAATTTTGTTTAAAAGATCACTCAGAGAAGTTACAAAAAGTTCTATTAATTACGTGGCGAATTGTGGGCCCTTTCTCATTGACAAGGAACATATCTCAGGGTAGATGGAAGTTAGAGGAGAGAGCAAAAGATGAGCTGCTCATGCAGGCTGCTTCATTGAGAAAGGAGAGAGAACAAATAGAAGCTTCAGTGAAAGCAAAGGAGGATACAATTAAATTGAAAGCAGAAAACAATCTTTTAAAATACAAAGACGATATCCAAAAGCTTGAAAAAGAAATCTCTCAGTTGAGACTTAAGACTGATTCTTCAAGAATTGCTGCTCTTAAGAGGGGCATCGACGGAAGTTATGCCAGTAGGCTTACAGATACCAGAAACAGCACAGATCACAAAGAGTCCTGGACCCCGAATGTCTCGGAATCGATGAAGGATCTTTACGAGTACTCTGGAACTGGAGGCGTGAAGCGGGAACGGGAGTGTGTGATGTGCCTTTCAGAGGAGATGTCAGTAGTTTTTCTTCCTTGCGCCCATCAGGTGGTGTGCACAACCTGCAATGAACTACATGAAAAACAGGGTATGAAAGATTGTCCTTCTTGTAGGAGCCCGATACAGCGGCGTATTCCAGTCCGTTATGCTCGCTCGTAA

Protein sequence

MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDADWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDMNVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSSQSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLPAPSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLRATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQLEMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIVGPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKYKDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMKDLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYARS
Homology
BLAST of Spg031971 vs. NCBI nr
Match: KAA0049418.1 (putative E3 ubiquitin-protein ligase RF298 [Cucumis melo var. makuwa] >TYK16096.1 putative E3 ubiquitin-protein ligase RF298 [Cucumis melo var. makuwa])

HSP 1 Score: 1529.2 bits (3958), Expect = 0.0e+00
Identity = 826/971 (85.07%), Postives = 862/971 (88.77%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDA 120
           AEKFEISSS+GQ+  CDLC ISQEFSAGLKLDLGLSNGGSSDVG+NWPRGELEVDE+QDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEAQLEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDM 240
           DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLI DM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSS 300
           NVS ACAMDS+P NA VCDG  NES  N++PQLKAE KSSEM+LPKPVKP+SPIS AH S
Sbjct: 241 NVSLACAMDSNPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGS 360
           QSDGPA +GVP+++KPK+PLFSSGP+S+KELQNST DV EESFSVA NSQTSVSEEKI S
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLP 480
           VNFKNASLKISKAMGIDVAQDNGSHNLST+DIPSSSL FNLENINTVS FSKTN+ SS+P
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 APSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA N SSA P  DIDLSLSLPTKSN PS+P +CN ESSTSSFVEKP+EK +G
Sbjct: 481 APSSPPALPAMNTSSAPPTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVL L+PR +ELQNQLQEWT+WANQKVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQ+MEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRK+K+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIV 780
           E                                                           
Sbjct: 721 E----------------------------------------------------------- 780

Query: 781 GPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKY 840
                      GRWKLEERAKDELL+QAASLRKEREQIE SVK KEDTIKLKAENNL+KY
Sbjct: 781 -----------GRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKY 840

Query: 841 KDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMK 900
           KDDIQKLEKEIS LRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESW+PNVSESMK
Sbjct: 841 KDDIQKLEKEISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMK 900

Query: 901 DLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 960
           DLY+YSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI
Sbjct: 901 DLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 901

Query: 961 QRRIPVRYARS 972
           QRRIPVRYARS
Sbjct: 961 QRRIPVRYARS 901

BLAST of Spg031971 vs. NCBI nr
Match: XP_008438753.1 (PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Cucumis melo])

HSP 1 Score: 1528.1 bits (3955), Expect = 0.0e+00
Identity = 826/971 (85.07%), Postives = 861/971 (88.67%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHG SSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGSSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDA 120
           AEKFEISSS+GQ+  CDLC ISQEFSAGLKLDLGLSNGGSSDVG+NWPRGELEVDE+QDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEAQLEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDM 240
           DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLI DM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSS 300
           NVS ACAMDSDP NA VCDG  NES  N++PQLKAE KSSEM+LPKPVKP+SPIS AH S
Sbjct: 241 NVSLACAMDSDPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGS 360
           QSDGPA +GVP+++KPK+PLFSSGP+S+KELQNST DV EESFSVA NSQTSVSEEKI S
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLP 480
           VNFKNASLKISKAMGIDVAQDNGSHNLST+DIPSSSL FNLENINTVS FSKTN+ SS+P
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 APSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA N SSA P  DIDLSLSLPTKSN PS+P +CN ESSTSSFVEKP+EK +G
Sbjct: 481 APSSPPALPAMNTSSAPPTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVL L+PR +ELQNQLQEWT+WANQKVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQ+MEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRK+K+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIV 780
           E                                                           
Sbjct: 721 E----------------------------------------------------------- 780

Query: 781 GPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKY 840
                      GRWKLEERAKDELL+QAASLRKEREQIE SVK KEDTIKLKAENNL+KY
Sbjct: 781 -----------GRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKY 840

Query: 841 KDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMK 900
           KDDIQKLEKEIS LRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESW+PNVSESMK
Sbjct: 841 KDDIQKLEKEISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMK 900

Query: 901 DLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 960
           DLY+YSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI
Sbjct: 901 DLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 901

Query: 961 QRRIPVRYARS 972
           QRRIPVRYARS
Sbjct: 961 QRRIPVRYARS 901

BLAST of Spg031971 vs. NCBI nr
Match: XP_004134170.2 (putative E3 ubiquitin-protein ligase RF298 [Cucumis sativus] >KGN57044.1 hypothetical protein Csa_009823 [Cucumis sativus])

HSP 1 Score: 1526.9 bits (3952), Expect = 0.0e+00
Identity = 825/971 (84.96%), Postives = 861/971 (88.67%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDA 120
           AEKFEISSS+GQ++ CDLCSISQEFSAGLKLDLGLSNGGSSDVG+NWPRGELEVDE QDA
Sbjct: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120

Query: 121 DWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEAQLEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDM 240
           DNTLAFLR GQEIDHSREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLI DM
Sbjct: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSS 300
           +V+ ACAMDSDP NA VCDG  NESSSN+IPQLKAE KSSEM+LPKPVKP+SPISCAH S
Sbjct: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300

Query: 301 QSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGS 360
           Q DGPA +GVP+++KPK+PLFSSGP+S+KELQNST DV EESFSVA NSQTSVSEEKI S
Sbjct: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYG KGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLP 480
           VNFKNASLKISKAMGIDVAQDNGSHNLST+DIPSSSL FNLENINTVSPFSKTN+ SS+P
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLPSSMP 480

Query: 481 APSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA N SSA P  DIDLSLSLP KSN PS+P +CN ESSTSSFVEKP EK +G
Sbjct: 481 APSSPPALPALNTSSAPPTTDIDLSLSLPAKSNQPSVPFNCNPESSTSSFVEKPQEKFIG 540

Query: 541 QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVL L+PR +ELQNQLQEWT+WANQKVMQAARRLSKDKAELK LKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKALKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQ+MEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRK+K+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIV 780
           E                                                           
Sbjct: 721 E----------------------------------------------------------- 780

Query: 781 GPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKY 840
                      GRWKLEERAKDELL+QAASLRKEREQIE SVK KEDTIKLKAENNL+KY
Sbjct: 781 -----------GRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKY 840

Query: 841 KDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMK 900
           KDDIQKLEKEIS LRLKTDSSRIAALKRGIDGSYASRLTDTRN+TDHKESW+PNVSESMK
Sbjct: 841 KDDIQKLEKEISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMK 900

Query: 901 DLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 960
           DLY+YSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI
Sbjct: 901 DLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 901

Query: 961 QRRIPVRYARS 972
           QRRIPVRYARS
Sbjct: 961 QRRIPVRYARS 901

BLAST of Spg031971 vs. NCBI nr
Match: XP_022137773.1 (putative E3 ubiquitin-protein ligase RF298 isoform X1 [Momordica charantia])

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 831/972 (85.49%), Postives = 864/972 (88.89%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNH PSSMTVQEKGSRNKRKYRADPPLGDLNKIT SSQDECPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHVPSSMTVQEKGSRNKRKYRADPPLGDLNKITMSSQDECPSYEFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNG-GSSDVGLNWPRGELEVDEFQD 120
           AEKFEISS++GQT+ACD+CSISQE+SA LKLDLGLSNG GSSDVGLNWPR EL+VDE +D
Sbjct: 61  AEKFEISSNIGQTSACDICSISQEYSAALKLDLGLSNGLGSSDVGLNWPREELDVDEVED 120

Query: 121 ADWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNI 180
           ADWSDLTEAQLEELVLSNLDTIFKSA KKIVASGY+EEVAIKAVSR+GICFG KDTVSNI
Sbjct: 121 ADWSDLTEAQLEELVLSNLDTIFKSATKKIVASGYSEEVAIKAVSRAGICFGSKDTVSNI 180

Query: 181 VDNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD 240
           VDNTLAFLRSGQEIDHSREH FEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD
Sbjct: 181 VDNTLAFLRSGQEIDHSREHCFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD 240

Query: 241 MNVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHS 300
           MNV+HACAMDSDPFNAF CDGA NESSSNSIPQ+KAEAK SEM+LPKPVKP+SPISCAHS
Sbjct: 241 MNVTHACAMDSDPFNAFFCDGASNESSSNSIPQIKAEAKISEMNLPKPVKPISPISCAHS 300

Query: 301 SQSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIG 360
           SQS+ PA LGVPNL K K+P+F SGPVSDKE Q+S SDVAEESF+VAGNSQTSVSEEKIG
Sbjct: 301 SQSEEPATLGVPNLPKTKDPVFLSGPVSDKEFQSSASDVAEESFNVAGNSQTSVSEEKIG 360

Query: 361 SSRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGST 420
           SSRK HSNITKREYMLRQKSLHV+KNFRTYGPKGSSR GKLTGLGGLMLDKK+KSVS ST
Sbjct: 361 SSRKFHSNITKREYMLRQKSLHVEKNFRTYGPKGSSRGGKLTGLGGLMLDKKIKSVSSST 420

Query: 421 AVNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSL 480
           +VNFKNAS KISKAMGIDV QD G+H+LSTIDIP+SSL+F LENI+T+SPFSK N LSS+
Sbjct: 421 SVNFKNAS-KISKAMGIDVIQDIGNHSLSTIDIPASSLAF-LENISTISPFSKANTLSSM 480

Query: 481 PAPSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSL 540
           PAPSSPPALP  N SSAL  +DIDLSLSLPTKSN PSMPIS NAESS+SSFVEK YEKSL
Sbjct: 481 PAPSSPPALPEANTSSALSTSDIDLSLSLPTKSNQPSMPISYNAESSSSSFVEKSYEKSL 540

Query: 541 GQWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKE 600
           GQWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELK LKQEKE
Sbjct: 541 GQWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKALKQEKE 600

Query: 601 EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKL 660
           EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKL
Sbjct: 601 EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKL 660

Query: 661 RATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQ 720
           RATESAASYQEVS+REKKTLMKVQSWEKQK LFQEEHTAEKRKLKQLIQEL+QARDLQEQ
Sbjct: 661 RATESAASYQEVSRREKKTLMKVQSWEKQKTLFQEEHTAEKRKLKQLIQELDQARDLQEQ 720

Query: 721 LEMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRI 780
           LE                                                          
Sbjct: 721 LE---------------------------------------------------------- 780

Query: 781 VGPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLK 840
                       GRWKLEERAKDE+LMQAASLRKEREQIEASVKAKEDTIKLKAENNLLK
Sbjct: 781 ------------GRWKLEERAKDEVLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLK 840

Query: 841 YKDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESM 900
           YKDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESM
Sbjct: 841 YKDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESM 900

Query: 901 KDLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSP 960
           KDLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSP
Sbjct: 901 KDLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSP 900

Query: 961 IQRRIPVRYARS 972
           IQRRIPVRYARS
Sbjct: 961 IQRRIPVRYARS 900

BLAST of Spg031971 vs. NCBI nr
Match: KAG7028938.1 (putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1510.0 bits (3908), Expect = 0.0e+00
Identity = 816/971 (84.04%), Postives = 854/971 (87.95%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCP+TSNHGPSS+ VQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSY+FS
Sbjct: 1   MASMVAKPSCPATSNHGPSSVLVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYDFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDA 120
           AEKFEISSSLGQ  ACDLCSISQEFSAGLKLDLGLSNGGS DVG++WPRGELEVDEFQD 
Sbjct: 61  AEKFEISSSLGQIGACDLCSISQEFSAGLKLDLGLSNGGSPDVGIDWPRGELEVDEFQDV 120

Query: 121 DWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEAQL EL+L NLDTIFK+AIKK+VASG+TEEVAIKAVSRSGICFGCKD +SN+V
Sbjct: 121 DWSDLTEAQLVELILCNLDTIFKAAIKKVVASGFTEEVAIKAVSRSGICFGCKDIMSNVV 180

Query: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDM 240
           DNTLAFLRSGQEID SREHYFEDLQQLEKYILAELVCVLREVRP+FSTGDAMWCLL+ DM
Sbjct: 181 DNTLAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPYFSTGDAMWCLLVSDM 240

Query: 241 NVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSS 300
           +VSHAC MDSDP NA VCDG  NE S+NS PQLKAEAKSSE++ PKP+KP+SPISCAHSS
Sbjct: 241 SVSHACTMDSDPSNAGVCDGTSNEGSTNSTPQLKAEAKSSELNFPKPLKPISPISCAHSS 300

Query: 301 QSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGS 360
           QSDGPA LGVPNLTKPK+PLFSS  VSDKELQNSTSDVA ESF+VAGN QTSV+EEKIGS
Sbjct: 301 QSDGPATLGVPNLTKPKDPLFSSVSVSDKELQNSTSDVARESFNVAGNPQTSVAEEKIGS 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SR+VHSNITKREYMLRQKSLHVDKNFRTYGPKG SRAGK+TGLG LM+DKKLKSVSGSTA
Sbjct: 361 SRRVHSNITKREYMLRQKSLHVDKNFRTYGPKGPSRAGKVTGLGSLMVDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLP 480
           VNFKNASLKISKAMGIDVAQDNG+HN +TIDIPSSSLSFNLEN  T SPFSK NVLSS+P
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGNHNPTTIDIPSSSLSFNLENNKTASPFSKINVLSSMP 480

Query: 481 APSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLG 540
           APSSP ALPATN SSALPAA+ DLSLSLPTKSN PS+PISCNAE STSSFVEKPYEKSLG
Sbjct: 481 APSSPLALPATNTSSALPAAETDLSLSLPTKSNEPSVPISCNAEPSTSSFVEKPYEKSLG 540

Query: 541 QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFP+DKKDEMVLKLVPRARELQ+QLQEW EWANQKVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPKDKKDEMVLKLVPRARELQSQLQEWMEWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660
           VERLKKEKQTLEENT KKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR
Sbjct: 601 VERLKKEKQTLEENTKKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQL 720
           ATESAA++QEVSKREKKTL+ VQSWEK KMLFQEEHT EKRKLKQLIQELEQARD+QEQL
Sbjct: 661 ATESAATFQEVSKREKKTLVNVQSWEKTKMLFQEEHTVEKRKLKQLIQELEQARDVQEQL 720

Query: 721 EMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIV 780
           E                                                           
Sbjct: 721 E----------------------------------------------------------- 780

Query: 781 GPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKY 840
                      GR K+E RAKDELLMQAASLRKEREQIEAS+KAKEDTIKLKAENNLLKY
Sbjct: 781 -----------GRGKMEARAKDELLMQAASLRKEREQIEASLKAKEDTIKLKAENNLLKY 840

Query: 841 KDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMK 900
           K DIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRL DTRNSTDH ESWTPNVSESMK
Sbjct: 841 KSDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLIDTRNSTDHNESWTPNVSESMK 900

Query: 901 DLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 960
           DLYEYSGTG VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI
Sbjct: 901 DLYEYSGTGSVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 901

Query: 961 QRRIPVRYARS 972
           QRRIPVRY RS
Sbjct: 961 QRRIPVRYTRS 901

BLAST of Spg031971 vs. ExPASy Swiss-Prot
Match: Q0WPJ7 (Putative E3 ubiquitin-protein ligase RF298 OS=Arabidopsis thaliana OX=3702 GN=RF298 PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 6.6e-126
Identity = 355/951 (37.33%), Postives = 503/951 (52.89%), Query Frame = 0

Query: 24  QEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSLGQTNACDLCSISQ 83
           Q+KG +NKRK      L D +   ++S  E P YE  + K        Q+  C+      
Sbjct: 29  QDKGRKNKRK------LADPSPQNAASLTEFPRYELHSFK-------SQSPLCE------ 88

Query: 84  EFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDADWSDLTEAQLEELVLSNLDTIFK 143
                                 N   G+L+ +E     W D     LE L+ SNL T+F+
Sbjct: 89  ----------------------NDSNGQLKAEESDSVGWDDPFACHLEGLLSSNLLTLFR 148

Query: 144 SAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNTLAFLRSGQEIDHSREHYFED 203
           SA+ +I+  GY+E+V +KA+S S    G  D VSNIV++TL+FL+SG+++  SR++ FED
Sbjct: 149 SAMNQIMDCGYSEDVVLKAISSSRFYCGGTDLVSNIVNDTLSFLKSGKKVAGSRDYVFED 208

Query: 204 LQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDMNVSHACAMDSDPFNAFVCDGALN 263
           LQQL  Y L E + ++REVRP  ST +AMW LLICD+NV  A  +D+D           N
Sbjct: 209 LQQLVAYSLVEKISLVREVRPSLSTDEAMWRLLICDLNVLKAFEVDADGLEG---SSVSN 268

Query: 264 ESSSNSIPQLKAE-AKSSEMSLPKPVKPVSPISCAHSSQSDGPAALGVPNLTKPKEPLFS 323
            S S+  P  +    KSS+   PK     +P+S   S QS+        N+   K P  +
Sbjct: 269 ASKSSESPVAECNPPKSSDADNPK-----APVSNTQSKQSEPVKFGNFANVNNSKNP-HA 328

Query: 324 SGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQKSLH 383
           SG    KE+ + ++   E + S    S TSVS+EK+ S RK     TK+E  MLRQKS  
Sbjct: 329 SGATPGKEVFSVSTASGEGTKSA---SLTSVSDEKLVSCRK---GRTKKEMAMLRQKSC- 388

Query: 384 VDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNASLKISKAMGIDVAQD 443
           V+K  RTY   G  +  K    GG +++K+ KS S   +   +N+S KI+          
Sbjct: 389 VEK-IRTYSKGGGYKTAK---FGGFLVEKRGKSASDLLSAQARNSSSKIT---------- 448

Query: 444 NGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLPAPSSPPALPATNISSALPAAD 503
                   + IP +  S  L N                   S  PAL      +ALPA +
Sbjct: 449 -----TEVMKIPLAESSSTLSN----------------STKSDSPALDVKEHVTALPANN 508

Query: 504 IDLSLSLPTKS-NLPSMPISCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMVLKLVPRAR 563
               ++   KS + P    S + + +   +   PY+ +LG + PR+K+DE++LKLVPR +
Sbjct: 509 APAPVASEKKSGSEPEEKPSVSTKPAPDYYAAIPYDATLGIYIPRNKRDELILKLVPRMK 568

Query: 564 ELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLEENTMKKLS 623
           +LQ +LQ+WT+WANQKV QA  RL KD+ ELK L++EKEE E  +KEKQ LEENT+K+ S
Sbjct: 569 DLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLEENTIKRRS 628

Query: 624 EMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLRATESAASYQEVSKREKKTLM 683
           EME AL  A+ Q+E  N+ +RRLE+E + L++E E A +RA+ESA S +E  +R ++ L 
Sbjct: 629 EMELALNNATNQLERTNNTIRRLELEQSLLKREREAANIRASESAESCREAKERVQRLLK 688

Query: 684 KVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQLEMVQKAITALGMESMVVVSY 743
             QSWE QK L QEE  +++ K+  L QE+ +A+  Q Q+E                   
Sbjct: 689 NSQSWEGQKNLLQEELKSQRDKVAGLQQEVAKAKTRQNQIE------------------- 748

Query: 744 CTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIVGPFSLTRNISQGRWKLEERA 803
                                                                WK E+ A
Sbjct: 749 ---------------------------------------------------ATWKQEKSA 808

Query: 804 KDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKYKDDIQKLEKEISQLRLKTDS 863
             +L  QAA+L+KER ++E   KA+E+ IK KAEN++  Y ++I++L+ EIS+L+LK+DS
Sbjct: 809 TGKLTAQAAALKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDS 813

Query: 864 SRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMKDLYEYSGT-GGVKRERECVM 923
            +IAALK+GIDG+     +   ++T+ K     N   S K      G    +KRERECVM
Sbjct: 869 LKIAALKKGIDGNNDGNKSGMNHTTNTK----ANSMASAKVWENNQGAESKIKRERECVM 813

Query: 924 CLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 971
           CLSEEMSV+FLPCAHQV+C+ CN+LHEK+ M+DCPSCR+ IQRRI  R+AR
Sbjct: 929 CLSEEMSVIFLPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 813

BLAST of Spg031971 vs. ExPASy Swiss-Prot
Match: Q9ZVT8 (Putative E3 ubiquitin-protein ligase RF4 OS=Arabidopsis thaliana OX=3702 GN=RF4 PE=3 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.2e-116
Identity = 344/956 (35.98%), Postives = 498/956 (52.09%), Query Frame = 0

Query: 20  SMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSLGQTNACDLC 79
           +++ QEKG +NKRK      L D ++  +SS  E P YE  + K                
Sbjct: 24  TVSPQEKGRKNKRK------LADPSQPNASSLTEFPPYELPSLK---------------- 83

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDADWSDLTEAQLEELVLSNLD 139
              Q   +G        NG   +V       +L+V+  +  +W D     LEEL+ SNL 
Sbjct: 84  --PQNHLSG--------NGSVGEVS-----NQLQVEVSESVEWDDPFACHLEELLSSNLL 143

Query: 140 TIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNTLAFLRSGQEIDHSREH 199
           T+F   +K+++  GYT++  +KAVSR  +  G  + +SNIV+NTL+ L++G E   S ++
Sbjct: 144 TLFLDTMKQLIDLGYTDDEVLKAVSRCRLYCGGNNLLSNIVNNTLSALKTGDEGAGSGDY 203

Query: 200 YFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDMNVSHACAMDSDPFNAFVCD 259
            FEDLQQL  Y L E++ +++EVRP  ST +AMW LL+CD+NV  A   +         D
Sbjct: 204 VFEDLQQLVSYTLVEMISLIKEVRPSLSTVEAMWRLLMCDLNVLQAFEAEG--------D 263

Query: 260 GALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSSQSDGPAALGVPNLTKPKEP 319
           G ++ S  +    L AE+   + S P   KP  P S   S++++       PN    K+ 
Sbjct: 264 GLVSSSKLSDSESLGAESNPPKSSDPDNPKP--PQSDPQSNRNEPLKFGNFPNTPNSKKT 323

Query: 320 LFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQK 379
             SSG    KE+ + ++   +    +   S T VS+EK+ S RK     TK+E  MLRQK
Sbjct: 324 Q-SSGTTPGKEVCSGSTVSCQ---GMRSTSFTLVSDEKLVSCRK---GRTKKEIAMLRQK 383

Query: 380 SLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNASLKISKAMGIDV 439
           S  V+K  RTY      +A K   +G  +L+K++KS S       +N+S KI+  +G+ V
Sbjct: 384 SC-VEK-IRTYSKGSGYKAAKFASVGSFLLEKRVKSSSEFVP---RNSSSKITAEIGVKV 443

Query: 440 --AQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLPAPSSPPALPATNISSA 499
             A+D+G        + S  +  + +   T  P       S     S    L     S++
Sbjct: 444 SLAEDSGCFVRKNSKLDSPVVVVDAKGYITALPARSVKSASKKKTGSESVTLIP---SAS 503

Query: 500 LPAADIDLSLSLPTKSNLPS-MPISCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMVLKL 559
              +D  +  +   KS   S    S +A+ +   +   PY+ +LG + PRDKKDE++LKL
Sbjct: 504 EKKSDSSIPSTSEKKSGSESEEKASVSAKLAPDYYAGIPYDAALGIYVPRDKKDELILKL 563

Query: 560 VPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLEENT 619
           VPR  +LQN+LQ WT+WANQKV +A  RL KD+ ELK L++E+EE E+ KKEKQ LEENT
Sbjct: 564 VPRVNDLQNELQVWTDWANQKVKEATGRLLKDQPELKALRKEREEAEQYKKEKQLLEENT 623

Query: 620 MKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLRATESAASYQEVSKRE 679
            K+LSEM+ AL  A+ Q+E A +   RLE+E + L++EME AK++A ESA S++E  +R 
Sbjct: 624 RKRLSEMDFALKNATSQLEKAFNTAHRLELEQSILKKEMEAAKIKAVESAESFREAKERG 683

Query: 680 KKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQLEMVQKAITALGMESM 739
           +++L  + SWE QK++ QEE   ++ K+  L +E+ +A++ Q Q+E   K          
Sbjct: 684 ERSLKDIHSWEGQKIMLQEELKGQREKVTVLQKEVTKAKNRQNQIEAALKQ--------- 743

Query: 740 VVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIVGPFSLTRNISQGRWK 799
                                                                       
Sbjct: 744 ------------------------------------------------------------ 803

Query: 800 LEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKYKDDIQKLEKEISQLR 859
            E  AK +L  QA+ +RKE +++EA  K +E+ IK KAE ++  Y D+I++LE+EIS+L+
Sbjct: 804 -ERTAKGKLSAQASLIRKETKELEALGKVEEERIKGKAETDVKYYIDNIKRLEREISELK 822

Query: 860 LKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMKDLYEYSGTGGVKRER 919
           LK+D SRI ALK+G                          SES     E  G   VKRER
Sbjct: 864 LKSDYSRIIALKKG-------------------------SSESKATKRESLGMPKVKRER 822

Query: 920 ECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYARS 972
           ECVMCLSEEMSV+FLPCAHQV+C  CN+LHEK+GM DCPSCR  I RRI  R+ARS
Sbjct: 924 ECVMCLSEEMSVIFLPCAHQVLCFKCNQLHEKEGMMDCPSCRGTIHRRIQARFARS 822

BLAST of Spg031971 vs. ExPASy Swiss-Prot
Match: Q8RX22 (MND1-interacting protein 1 OS=Arabidopsis thaliana OX=3702 GN=MIP1 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 8.0e-55
Identity = 240/908 (26.43%), Postives = 393/908 (43.28%), Query Frame = 0

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDADWSDLTEAQLEELVLSNLD 139
           ++SQ     L    GL++ GS  V LN        +  ++  W+  TE  LEE++L +L+
Sbjct: 34  AMSQSIVENLVYHPGLTDSGS--VNLN-----SVTENPEENFWAYCTEEHLEEILLKHLE 93

Query: 140 TIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNTLAFLRSG-------QE 199
            ++  A+ K++  GY E VA+KAV  +G C+G  D ++NIV+N+L++L SG         
Sbjct: 94  FLYNQAVSKLLELGYEERVALKAVLSNGHCYGELDVLTNIVNNSLSYLNSGGGGGGSNGN 153

Query: 200 IDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDMNVSHACAMDSDP 259
            +   E  F DL+ LE+Y LA ++ +L++V+P  S GDAMWCLL+ +++V  A  +D   
Sbjct: 154 GEDRTETGFTDLRDLEEYSLAGMIYLLQQVKPNLSKGDAMWCLLMSELHVGRASTLD--- 213

Query: 260 FNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSSQSDGPAALGVPN 319
                                                P +  SC     S+         
Sbjct: 214 ------------------------------------VPTNRSSCCTKEDSN--------- 273

Query: 320 LTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGSSRKVHSNITKRE 379
                E + + G                 +  +AG    ++                   
Sbjct: 274 ----VEDVGTGG-----------------TLDIAGFMAPALC------------------ 333

Query: 380 YMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNASLKISK 439
                         R +G  G    G                 SG+   + K A LK+ +
Sbjct: 334 --------------RFHGGWGFGNGGG-------------PEFSGN-GFSMKGAELKLQR 393

Query: 440 AMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLPAPSSPPALPATN 499
                            ID P     FNL    ++    K NV +   A     ++    
Sbjct: 394 ----------------EIDCPK---RFNLS--PSMKSLLKRNVAAF--AAGYRASMKQKQ 453

Query: 500 ISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLGQWFPR---DKKD 559
           I S+    D           +    P    +E S S+ +EK  + +L         D KD
Sbjct: 454 IQSSDTIGDSKACNDPAIVKSCGQQPRKSGSEESVSTVLEKFRDLNLDDNLESVGVDDKD 513

Query: 560 EMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQ 619
            +++ L+ + ++ + +++E  EWA +  MQAA+++S++ AELKTL  E+E ++ LKK KQ
Sbjct: 514 CVIVDLLHQVKDFEKKVKERKEWAQKNAMQAAQKVSEELAELKTLSSEREGIQLLKKGKQ 573

Query: 620 TLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLRATESAASYQ 679
            +EE+T K+ ++ E  L KA  Q + AN  VR+LE +NA +R E E +KL A+ES  +  
Sbjct: 574 AVEESTAKRFTDKEIELRKACSQNDRANVIVRKLENQNAEIRAEREGSKLSASESLKACM 633

Query: 680 EVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQLEMVQKAITA 739
           E SK+EKK L K+ +WEKQ +  Q+E TAEK K+K L + L Q  + ++++E        
Sbjct: 634 EASKKEKKCLKKLVAWEKQILKLQDEITAEKEKIKALYKTLAQITEYEKEIE-------- 693

Query: 740 LGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIVGPFSLTRNI 799
                                                                       
Sbjct: 694 ------------------------------------------------------------ 711

Query: 800 SQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKYKDDIQKLEK 859
              +W+ E++AK+E L Q    ++ +E  E   K K +T++LK E +  ++KDD Q+LE+
Sbjct: 754 --AKWRQEQKAKEEALAQMEEEQRSKEAAEGHNKRKLETLRLKIELDFQRHKDDHQRLEQ 711

Query: 860 EISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMKDLYEYSGT- 919
           E+ +L+  +DS          D S+ S      N+   K+S   N+++ ++++ +  G+ 
Sbjct: 814 ELGRLKASSDS----------DSSHIS-----NNAWKPKKSQGENIAKLLEEIDKLEGSY 711

Query: 920 -GGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNE---LHEKQGMK-DCPSCRSPIQRR 972
                 +REC++C+ +E+SVVFLPCAHQVVC +C++        G K  CP CR  +Q+R
Sbjct: 874 DNEANYDRECIICMKDEVSVVFLPCAHQVVCGSCSDSFFASNNGGSKVTCPCCRGLVQQR 711

BLAST of Spg031971 vs. ExPASy TrEMBL
Match: A0A5D3D0B8 (Putative E3 ubiquitin-protein ligase RF298 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00180 PE=4 SV=1)

HSP 1 Score: 1529.2 bits (3958), Expect = 0.0e+00
Identity = 826/971 (85.07%), Postives = 862/971 (88.77%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDA 120
           AEKFEISSS+GQ+  CDLC ISQEFSAGLKLDLGLSNGGSSDVG+NWPRGELEVDE+QDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEAQLEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDM 240
           DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLI DM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSS 300
           NVS ACAMDS+P NA VCDG  NES  N++PQLKAE KSSEM+LPKPVKP+SPIS AH S
Sbjct: 241 NVSLACAMDSNPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGS 360
           QSDGPA +GVP+++KPK+PLFSSGP+S+KELQNST DV EESFSVA NSQTSVSEEKI S
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLP 480
           VNFKNASLKISKAMGIDVAQDNGSHNLST+DIPSSSL FNLENINTVS FSKTN+ SS+P
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 APSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA N SSA P  DIDLSLSLPTKSN PS+P +CN ESSTSSFVEKP+EK +G
Sbjct: 481 APSSPPALPAMNTSSAPPTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVL L+PR +ELQNQLQEWT+WANQKVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQ+MEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRK+K+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIV 780
           E                                                           
Sbjct: 721 E----------------------------------------------------------- 780

Query: 781 GPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKY 840
                      GRWKLEERAKDELL+QAASLRKEREQIE SVK KEDTIKLKAENNL+KY
Sbjct: 781 -----------GRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKY 840

Query: 841 KDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMK 900
           KDDIQKLEKEIS LRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESW+PNVSESMK
Sbjct: 841 KDDIQKLEKEISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMK 900

Query: 901 DLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 960
           DLY+YSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI
Sbjct: 901 DLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 901

Query: 961 QRRIPVRYARS 972
           QRRIPVRYARS
Sbjct: 961 QRRIPVRYARS 901

BLAST of Spg031971 vs. ExPASy TrEMBL
Match: A0A1S3AX62 (putative E3 ubiquitin-protein ligase RF298 OS=Cucumis melo OX=3656 GN=LOC103483769 PE=4 SV=1)

HSP 1 Score: 1528.1 bits (3955), Expect = 0.0e+00
Identity = 826/971 (85.07%), Postives = 861/971 (88.67%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHG SSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGSSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDA 120
           AEKFEISSS+GQ+  CDLC ISQEFSAGLKLDLGLSNGGSSDVG+NWPRGELEVDE+QDA
Sbjct: 61  AEKFEISSSMGQSGGCDLCGISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEYQDA 120

Query: 121 DWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEAQLEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDM 240
           DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLI DM
Sbjct: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSS 300
           NVS ACAMDSDP NA VCDG  NES  N++PQLKAE KSSEM+LPKPVKP+SPIS AH S
Sbjct: 241 NVSLACAMDSDPCNALVCDGTSNESLPNTVPQLKAEVKSSEMNLPKPVKPISPISSAHGS 300

Query: 301 QSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGS 360
           QSDGPA +GVP+++KPK+PLFSSGP+S+KELQNST DV EESFSVA NSQTSVSEEKI S
Sbjct: 301 QSDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLP 480
           VNFKNASLKISKAMGIDVAQDNGSHNLST+DIPSSSL FNLENINTVS FSKTN+ SS+P
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSLFSKTNLPSSMP 480

Query: 481 APSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA N SSA P  DIDLSLSLPTKSN PS+P +CN ESSTSSFVEKP+EK +G
Sbjct: 481 APSSPPALPAMNTSSAPPTTDIDLSLSLPTKSNQPSVPFNCNPESSTSSFVEKPHEKFVG 540

Query: 541 QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVL L+PR +ELQNQLQEWT+WANQKVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQ+MEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRK+K+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIV 780
           E                                                           
Sbjct: 721 E----------------------------------------------------------- 780

Query: 781 GPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKY 840
                      GRWKLEERAKDELL+QAASLRKEREQIE SVK KEDTIKLKAENNL+KY
Sbjct: 781 -----------GRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKY 840

Query: 841 KDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMK 900
           KDDIQKLEKEIS LRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESW+PNVSESMK
Sbjct: 841 KDDIQKLEKEISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWSPNVSESMK 900

Query: 901 DLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 960
           DLY+YSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI
Sbjct: 901 DLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 901

Query: 961 QRRIPVRYARS 972
           QRRIPVRYARS
Sbjct: 961 QRRIPVRYARS 901

BLAST of Spg031971 vs. ExPASy TrEMBL
Match: A0A0A0L7S3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G150240 PE=4 SV=1)

HSP 1 Score: 1526.9 bits (3952), Expect = 0.0e+00
Identity = 825/971 (84.96%), Postives = 861/971 (88.67%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQD+CPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDKCPSYEFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDA 120
           AEKFEISSS+GQ++ CDLCSISQEFSAGLKLDLGLSNGGSSDVG+NWPRGELEVDE QDA
Sbjct: 61  AEKFEISSSMGQSSGCDLCSISQEFSAGLKLDLGLSNGGSSDVGINWPRGELEVDEDQDA 120

Query: 121 DWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEAQLEELVL NLDTIFK AIKKIVASGYTEEVAIKAVSRSGICFG KDTVSN+V
Sbjct: 121 DWSDLTEAQLEELVLINLDTIFKGAIKKIVASGYTEEVAIKAVSRSGICFGGKDTVSNVV 180

Query: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDM 240
           DNTLAFLR GQEIDHSREHYFEDLQQLEKYILAELVCVLRE+RPFFSTGDAMWCLLI DM
Sbjct: 181 DNTLAFLRRGQEIDHSREHYFEDLQQLEKYILAELVCVLREIRPFFSTGDAMWCLLISDM 240

Query: 241 NVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSS 300
           +V+ ACAMDSDP NA VCDG  NESSSN+IPQLKAE KSSEM+LPKPVKP+SPISCAH S
Sbjct: 241 SVALACAMDSDPCNALVCDGTSNESSSNTIPQLKAEVKSSEMNLPKPVKPISPISCAHGS 300

Query: 301 QSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGS 360
           Q DGPA +GVP+++KPK+PLFSSGP+S+KELQNST DV EESFSVA NSQTSVSEEKI S
Sbjct: 301 QYDGPATVGVPSISKPKDPLFSSGPLSEKELQNSTFDVVEESFSVAVNSQTSVSEEKIES 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SRKVHSNITKREYMLRQKSLHVDKNFRTYG KGSSRAGKLTGLGGLMLDKKLKSVSGSTA
Sbjct: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGAKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLP 480
           VNFKNASLKISKAMGIDVAQDNGSHNLST+DIPSSSL FNLENINTVSPFSKTN+ SS+P
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTMDIPSSSLPFNLENINTVSPFSKTNLPSSMP 480

Query: 481 APSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLG 540
           APSSPPALPA N SSA P  DIDLSLSLP KSN PS+P +CN ESSTSSFVEKP EK +G
Sbjct: 481 APSSPPALPALNTSSAPPTTDIDLSLSLPAKSNQPSVPFNCNPESSTSSFVEKPQEKFIG 540

Query: 541 QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFPRDKKDEMVL L+PR +ELQNQLQEWT+WANQKVMQAARRLSKDKAELK LKQEKEE
Sbjct: 541 QWFPRDKKDEMVLNLLPRVQELQNQLQEWTQWANQKVMQAARRLSKDKAELKALKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660
           VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQ+MEVAKLR
Sbjct: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQDMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQL 720
           ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRK+K+LIQELEQARDLQEQL
Sbjct: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKVKKLIQELEQARDLQEQL 720

Query: 721 EMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIV 780
           E                                                           
Sbjct: 721 E----------------------------------------------------------- 780

Query: 781 GPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKY 840
                      GRWKLEERAKDELL+QAASLRKEREQIE SVK KEDTIKLKAENNL+KY
Sbjct: 781 -----------GRWKLEERAKDELLVQAASLRKEREQIEDSVKVKEDTIKLKAENNLIKY 840

Query: 841 KDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMK 900
           KDDIQKLEKEIS LRLKTDSSRIAALKRGIDGSYASRLTDTRN+TDHKESW+PNVSESMK
Sbjct: 841 KDDIQKLEKEISVLRLKTDSSRIAALKRGIDGSYASRLTDTRNNTDHKESWSPNVSESMK 900

Query: 901 DLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 960
           DLY+YSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI
Sbjct: 901 DLYKYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 901

Query: 961 QRRIPVRYARS 972
           QRRIPVRYARS
Sbjct: 961 QRRIPVRYARS 901

BLAST of Spg031971 vs. ExPASy TrEMBL
Match: A0A6J1CBB5 (putative E3 ubiquitin-protein ligase RF298 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009134 PE=4 SV=1)

HSP 1 Score: 1522.3 bits (3940), Expect = 0.0e+00
Identity = 831/972 (85.49%), Postives = 864/972 (88.89%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCPSTSNH PSSMTVQEKGSRNKRKYRADPPLGDLNKIT SSQDECPSYEFS
Sbjct: 1   MASMVAKPSCPSTSNHVPSSMTVQEKGSRNKRKYRADPPLGDLNKITMSSQDECPSYEFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNG-GSSDVGLNWPRGELEVDEFQD 120
           AEKFEISS++GQT+ACD+CSISQE+SA LKLDLGLSNG GSSDVGLNWPR EL+VDE +D
Sbjct: 61  AEKFEISSNIGQTSACDICSISQEYSAALKLDLGLSNGLGSSDVGLNWPREELDVDEVED 120

Query: 121 ADWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNI 180
           ADWSDLTEAQLEELVLSNLDTIFKSA KKIVASGY+EEVAIKAVSR+GICFG KDTVSNI
Sbjct: 121 ADWSDLTEAQLEELVLSNLDTIFKSATKKIVASGYSEEVAIKAVSRAGICFGSKDTVSNI 180

Query: 181 VDNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD 240
           VDNTLAFLRSGQEIDHSREH FEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD
Sbjct: 181 VDNTLAFLRSGQEIDHSREHCFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICD 240

Query: 241 MNVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHS 300
           MNV+HACAMDSDPFNAF CDGA NESSSNSIPQ+KAEAK SEM+LPKPVKP+SPISCAHS
Sbjct: 241 MNVTHACAMDSDPFNAFFCDGASNESSSNSIPQIKAEAKISEMNLPKPVKPISPISCAHS 300

Query: 301 SQSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIG 360
           SQS+ PA LGVPNL K K+P+F SGPVSDKE Q+S SDVAEESF+VAGNSQTSVSEEKIG
Sbjct: 301 SQSEEPATLGVPNLPKTKDPVFLSGPVSDKEFQSSASDVAEESFNVAGNSQTSVSEEKIG 360

Query: 361 SSRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGST 420
           SSRK HSNITKREYMLRQKSLHV+KNFRTYGPKGSSR GKLTGLGGLMLDKK+KSVS ST
Sbjct: 361 SSRKFHSNITKREYMLRQKSLHVEKNFRTYGPKGSSRGGKLTGLGGLMLDKKIKSVSSST 420

Query: 421 AVNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSL 480
           +VNFKNAS KISKAMGIDV QD G+H+LSTIDIP+SSL+F LENI+T+SPFSK N LSS+
Sbjct: 421 SVNFKNAS-KISKAMGIDVIQDIGNHSLSTIDIPASSLAF-LENISTISPFSKANTLSSM 480

Query: 481 PAPSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSL 540
           PAPSSPPALP  N SSAL  +DIDLSLSLPTKSN PSMPIS NAESS+SSFVEK YEKSL
Sbjct: 481 PAPSSPPALPEANTSSALSTSDIDLSLSLPTKSNQPSMPISYNAESSSSSFVEKSYEKSL 540

Query: 541 GQWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKE 600
           GQWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELK LKQEKE
Sbjct: 541 GQWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKALKQEKE 600

Query: 601 EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKL 660
           EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKL
Sbjct: 601 EVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKL 660

Query: 661 RATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQ 720
           RATESAASYQEVS+REKKTLMKVQSWEKQK LFQEEHTAEKRKLKQLIQEL+QARDLQEQ
Sbjct: 661 RATESAASYQEVSRREKKTLMKVQSWEKQKTLFQEEHTAEKRKLKQLIQELDQARDLQEQ 720

Query: 721 LEMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRI 780
           LE                                                          
Sbjct: 721 LE---------------------------------------------------------- 780

Query: 781 VGPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLK 840
                       GRWKLEERAKDE+LMQAASLRKEREQIEASVKAKEDTIKLKAENNLLK
Sbjct: 781 ------------GRWKLEERAKDEVLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLK 840

Query: 841 YKDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESM 900
           YKDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESM
Sbjct: 841 YKDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESM 900

Query: 901 KDLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSP 960
           KDLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSP
Sbjct: 901 KDLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSP 900

Query: 961 IQRRIPVRYARS 972
           IQRRIPVRYARS
Sbjct: 961 IQRRIPVRYARS 900

BLAST of Spg031971 vs. ExPASy TrEMBL
Match: A0A6J1GLE6 (putative E3 ubiquitin-protein ligase RF298 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455308 PE=4 SV=1)

HSP 1 Score: 1507.3 bits (3901), Expect = 0.0e+00
Identity = 814/971 (83.83%), Postives = 852/971 (87.74%), Query Frame = 0

Query: 1   MASMVAKPSCPSTSNHGPSSMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFS 60
           MASMVAKPSCP+TSNHGPSS+ VQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSY+FS
Sbjct: 1   MASMVAKPSCPATSNHGPSSVLVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYDFS 60

Query: 61  AEKFEISSSLGQTNACDLCSISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDA 120
           AEKFEISSSLGQ  ACDLCSISQEFSAGLKLDLGLSNGGS DVG++WPRGELEVDEFQD 
Sbjct: 61  AEKFEISSSLGQIGACDLCSISQEFSAGLKLDLGLSNGGSPDVGIDWPRGELEVDEFQDV 120

Query: 121 DWSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIV 180
           DWSDLTEAQL EL+L NLDTIFK+AIKK+VASG+TEEVAIKA+SRSGICFGCKD +SN+V
Sbjct: 121 DWSDLTEAQLVELILCNLDTIFKAAIKKVVASGFTEEVAIKAISRSGICFGCKDIMSNVV 180

Query: 181 DNTLAFLRSGQEIDHSREHYFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDM 240
           DNTLAFLRSGQEID SREHYFEDLQQLEKYILAELVCVLREVRP+FSTGDAMWCLL+ DM
Sbjct: 181 DNTLAFLRSGQEIDQSREHYFEDLQQLEKYILAELVCVLREVRPYFSTGDAMWCLLVSDM 240

Query: 241 NVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSS 300
           +VSHAC MDSDP NA VCDG  NE S+NS PQLKAEAKSSE++ PKP+KP+SPISCAHSS
Sbjct: 241 SVSHACTMDSDPSNAGVCDGTSNEGSTNSTPQLKAEAKSSELNFPKPLKPISPISCAHSS 300

Query: 301 QSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGS 360
           QSDGPA LGVPNLTKPK+PLFSS  VSDKELQNS SDVA ESF+VAGN QTSV+EEKIGS
Sbjct: 301 QSDGPATLGVPNLTKPKDPLFSSVSVSDKELQNSASDVARESFNVAGNPQTSVAEEKIGS 360

Query: 361 SRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTA 420
           SR+VHSNITKREYMLRQKSLHVDKNFRTYGPKG SRAGK+TGLG LMLDKKLKSVSGSTA
Sbjct: 361 SRRVHSNITKREYMLRQKSLHVDKNFRTYGPKGPSRAGKVTGLGSLMLDKKLKSVSGSTA 420

Query: 421 VNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLP 480
           VNFKNASLKISKAMGIDVAQDNG+HN +TIDIPSSSLSFNLEN  T SPFSK NVLSS+P
Sbjct: 421 VNFKNASLKISKAMGIDVAQDNGNHNPTTIDIPSSSLSFNLENNKTASPFSKINVLSSMP 480

Query: 481 APSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSSFVEKPYEKSLG 540
           APSSP ALPATN SSALPAA+ DLSLSLPTKSN PS+PISCN E STSSFVEKPYEKSLG
Sbjct: 481 APSSPLALPATNTSSALPAAETDLSLSLPTKSNEPSVPISCNTEPSTSSFVEKPYEKSLG 540

Query: 541 QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEE 600
           QWFP+DKKDEMVLKLVPRARELQ+QLQEW EWANQKVMQAARRLSKDKAELKTLKQEKEE
Sbjct: 541 QWFPKDKKDEMVLKLVPRARELQSQLQEWMEWANQKVMQAARRLSKDKAELKTLKQEKEE 600

Query: 601 VERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660
           VERLKKEKQTLEENT KKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR
Sbjct: 601 VERLKKEKQTLEENTKKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLR 660

Query: 661 ATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQL 720
           ATESAA++QEVSKREKKTL+ VQSWEK KMLFQEEHT EKRKLKQLIQELEQARD+QEQL
Sbjct: 661 ATESAATFQEVSKREKKTLVNVQSWEKTKMLFQEEHTVEKRKLKQLIQELEQARDVQEQL 720

Query: 721 EMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIV 780
           E                                                           
Sbjct: 721 E----------------------------------------------------------- 780

Query: 781 GPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKY 840
                      GR K+E RAKDELLMQAASLRKEREQIEAS+KAKEDTIKLKAENNLLKY
Sbjct: 781 -----------GRGKMEARAKDELLMQAASLRKEREQIEASLKAKEDTIKLKAENNLLKY 840

Query: 841 KDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMK 900
           K DIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRL DTRNSTDH ESWTPNVSESMK
Sbjct: 841 KSDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLIDTRNSTDHNESWTPNVSESMK 900

Query: 901 DLYEYSGTGGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 960
           DLYEYSGTG VKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI
Sbjct: 901 DLYEYSGTGSVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPI 901

Query: 961 QRRIPVRYARS 972
           QRRIPVRY RS
Sbjct: 961 QRRIPVRYTRS 901

BLAST of Spg031971 vs. TAIR 10
Match: AT4G03000.1 (RING/U-box superfamily protein )

HSP 1 Score: 453.4 bits (1165), Expect = 4.7e-127
Identity = 355/951 (37.33%), Postives = 503/951 (52.89%), Query Frame = 0

Query: 24  QEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSLGQTNACDLCSISQ 83
           Q+KG +NKRK      L D +   ++S  E P YE  + K        Q+  C+      
Sbjct: 29  QDKGRKNKRK------LADPSPQNAASLTEFPRYELHSFK-------SQSPLCE------ 88

Query: 84  EFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDADWSDLTEAQLEELVLSNLDTIFK 143
                                 N   G+L+ +E     W D     LE L+ SNL T+F+
Sbjct: 89  ----------------------NDSNGQLKAEESDSVGWDDPFACHLEGLLSSNLLTLFR 148

Query: 144 SAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNTLAFLRSGQEIDHSREHYFED 203
           SA+ +I+  GY+E+V +KA+S S    G  D VSNIV++TL+FL+SG+++  SR++ FED
Sbjct: 149 SAMNQIMDCGYSEDVVLKAISSSRFYCGGTDLVSNIVNDTLSFLKSGKKVAGSRDYVFED 208

Query: 204 LQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDMNVSHACAMDSDPFNAFVCDGALN 263
           LQQL  Y L E + ++REVRP  ST +AMW LLICD+NV  A  +D+D           N
Sbjct: 209 LQQLVAYSLVEKISLVREVRPSLSTDEAMWRLLICDLNVLKAFEVDADGLEG---SSVSN 268

Query: 264 ESSSNSIPQLKAE-AKSSEMSLPKPVKPVSPISCAHSSQSDGPAALGVPNLTKPKEPLFS 323
            S S+  P  +    KSS+   PK     +P+S   S QS+        N+   K P  +
Sbjct: 269 ASKSSESPVAECNPPKSSDADNPK-----APVSNTQSKQSEPVKFGNFANVNNSKNP-HA 328

Query: 324 SGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQKSLH 383
           SG    KE+ + ++   E + S    S TSVS+EK+ S RK     TK+E  MLRQKS  
Sbjct: 329 SGATPGKEVFSVSTASGEGTKSA---SLTSVSDEKLVSCRK---GRTKKEMAMLRQKSC- 388

Query: 384 VDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNASLKISKAMGIDVAQD 443
           V+K  RTY   G  +  K    GG +++K+ KS S   +   +N+S KI+          
Sbjct: 389 VEK-IRTYSKGGGYKTAK---FGGFLVEKRGKSASDLLSAQARNSSSKIT---------- 448

Query: 444 NGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLPAPSSPPALPATNISSALPAAD 503
                   + IP +  S  L N                   S  PAL      +ALPA +
Sbjct: 449 -----TEVMKIPLAESSSTLSN----------------STKSDSPALDVKEHVTALPANN 508

Query: 504 IDLSLSLPTKS-NLPSMPISCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMVLKLVPRAR 563
               ++   KS + P    S + + +   +   PY+ +LG + PR+K+DE++LKLVPR +
Sbjct: 509 APAPVASEKKSGSEPEEKPSVSTKPAPDYYAAIPYDATLGIYIPRNKRDELILKLVPRMK 568

Query: 564 ELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLEENTMKKLS 623
           +LQ +LQ+WT+WANQKV QA  RL KD+ ELK L++EKEE E  +KEKQ LEENT+K+ S
Sbjct: 569 DLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLEENTIKRRS 628

Query: 624 EMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLRATESAASYQEVSKREKKTLM 683
           EME AL  A+ Q+E  N+ +RRLE+E + L++E E A +RA+ESA S +E  +R ++ L 
Sbjct: 629 EMELALNNATNQLERTNNTIRRLELEQSLLKREREAANIRASESAESCREAKERVQRLLK 688

Query: 684 KVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQLEMVQKAITALGMESMVVVSY 743
             QSWE QK L QEE  +++ K+  L QE+ +A+  Q Q+E                   
Sbjct: 689 NSQSWEGQKNLLQEELKSQRDKVAGLQQEVAKAKTRQNQIE------------------- 748

Query: 744 CTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIVGPFSLTRNISQGRWKLEERA 803
                                                                WK E+ A
Sbjct: 749 ---------------------------------------------------ATWKQEKSA 808

Query: 804 KDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKYKDDIQKLEKEISQLRLKTDS 863
             +L  QAA+L+KER ++E   KA+E+ IK KAEN++  Y ++I++L+ EIS+L+LK+DS
Sbjct: 809 TGKLTAQAAALKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDS 813

Query: 864 SRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMKDLYEYSGT-GGVKRERECVM 923
            +IAALK+GIDG+     +   ++T+ K     N   S K      G    +KRERECVM
Sbjct: 869 LKIAALKKGIDGNNDGNKSGMNHTTNTK----ANSMASAKVWENNQGAESKIKRERECVM 813

Query: 924 CLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 971
           CLSEEMSV+FLPCAHQV+C+ CN+LHEK+ M+DCPSCR+ IQRRI  R+AR
Sbjct: 929 CLSEEMSVIFLPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 813

BLAST of Spg031971 vs. TAIR 10
Match: AT4G03000.2 (RING/U-box superfamily protein )

HSP 1 Score: 453.4 bits (1165), Expect = 4.7e-127
Identity = 355/951 (37.33%), Postives = 503/951 (52.89%), Query Frame = 0

Query: 24  QEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSLGQTNACDLCSISQ 83
           Q+KG +NKRK      L D +   ++S  E P YE  + K        Q+  C+      
Sbjct: 29  QDKGRKNKRK------LADPSPQNAASLTEFPRYELHSFK-------SQSPLCE------ 88

Query: 84  EFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDADWSDLTEAQLEELVLSNLDTIFK 143
                                 N   G+L+ +E     W D     LE L+ SNL T+F+
Sbjct: 89  ----------------------NDSNGQLKAEESDSVGWDDPFACHLEGLLSSNLLTLFR 148

Query: 144 SAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNTLAFLRSGQEIDHSREHYFED 203
           SA+ +I+  GY+E+V +KA+S S    G  D VSNIV++TL+FL+SG+++  SR++ FED
Sbjct: 149 SAMNQIMDCGYSEDVVLKAISSSRFYCGGTDLVSNIVNDTLSFLKSGKKVAGSRDYVFED 208

Query: 204 LQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDMNVSHACAMDSDPFNAFVCDGALN 263
           LQQL  Y L E + ++REVRP  ST +AMW LLICD+NV  A  +D+D           N
Sbjct: 209 LQQLVAYSLVEKISLVREVRPSLSTDEAMWRLLICDLNVLKAFEVDADGLEG---SSVSN 268

Query: 264 ESSSNSIPQLKAE-AKSSEMSLPKPVKPVSPISCAHSSQSDGPAALGVPNLTKPKEPLFS 323
            S S+  P  +    KSS+   PK     +P+S   S QS+        N+   K P  +
Sbjct: 269 ASKSSESPVAECNPPKSSDADNPK-----APVSNTQSKQSEPVKFGNFANVNNSKNP-HA 328

Query: 324 SGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQKSLH 383
           SG    KE+ + ++   E + S    S TSVS+EK+ S RK     TK+E  MLRQKS  
Sbjct: 329 SGATPGKEVFSVSTASGEGTKSA---SLTSVSDEKLVSCRK---GRTKKEMAMLRQKSC- 388

Query: 384 VDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNASLKISKAMGIDVAQD 443
           V+K  RTY   G  +  K    GG +++K+ KS S   +   +N+S KI+          
Sbjct: 389 VEK-IRTYSKGGGYKTAK---FGGFLVEKRGKSASDLLSAQARNSSSKIT---------- 448

Query: 444 NGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLPAPSSPPALPATNISSALPAAD 503
                   + IP +  S  L N                   S  PAL      +ALPA +
Sbjct: 449 -----TEVMKIPLAESSSTLSN----------------STKSDSPALDVKEHVTALPANN 508

Query: 504 IDLSLSLPTKS-NLPSMPISCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMVLKLVPRAR 563
               ++   KS + P    S + + +   +   PY+ +LG + PR+K+DE++LKLVPR +
Sbjct: 509 APAPVASEKKSGSEPEEKPSVSTKPAPDYYAAIPYDATLGIYIPRNKRDELILKLVPRMK 568

Query: 564 ELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLEENTMKKLS 623
           +LQ +LQ+WT+WANQKV QA  RL KD+ ELK L++EKEE E  +KEKQ LEENT+K+ S
Sbjct: 569 DLQKELQDWTDWANQKVKQATVRLLKDQPELKALRKEKEEAEEFRKEKQLLEENTIKRRS 628

Query: 624 EMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLRATESAASYQEVSKREKKTLM 683
           EME AL  A+ Q+E  N+ +RRLE+E + L++E E A +RA+ESA S +E  +R ++ L 
Sbjct: 629 EMELALNNATNQLERTNNTIRRLELEQSLLKREREAANIRASESAESCREAKERVQRLLK 688

Query: 684 KVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQLEMVQKAITALGMESMVVVSY 743
             QSWE QK L QEE  +++ K+  L QE+ +A+  Q Q+E                   
Sbjct: 689 NSQSWEGQKNLLQEELKSQRDKVAGLQQEVAKAKTRQNQIE------------------- 748

Query: 744 CTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIVGPFSLTRNISQGRWKLEERA 803
                                                                WK E+ A
Sbjct: 749 ---------------------------------------------------ATWKQEKSA 808

Query: 804 KDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKYKDDIQKLEKEISQLRLKTDS 863
             +L  QAA+L+KER ++E   KA+E+ IK KAEN++  Y ++I++L+ EIS+L+LK+DS
Sbjct: 809 TGKLTAQAAALKKERGKLEELGKAEEERIKTKAENDVKYYIENIKRLDTEISKLKLKSDS 813

Query: 864 SRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMKDLYEYSGT-GGVKRERECVM 923
            +IAALK+GIDG+     +   ++T+ K     N   S K      G    +KRERECVM
Sbjct: 869 LKIAALKKGIDGNNDGNKSGMNHTTNTK----ANSMASAKVWENNQGAESKIKRERECVM 813

Query: 924 CLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYAR 971
           CLSEEMSV+FLPCAHQV+C+ CN+LHEK+ M+DCPSCR+ IQRRI  R+AR
Sbjct: 929 CLSEEMSVIFLPCAHQVLCSKCNQLHEKEAMEDCPSCRAKIQRRIQARFAR 813

BLAST of Spg031971 vs. TAIR 10
Match: AT1G03365.1 (RING/U-box superfamily protein )

HSP 1 Score: 422.5 bits (1085), Expect = 8.9e-118
Identity = 344/956 (35.98%), Postives = 498/956 (52.09%), Query Frame = 0

Query: 20  SMTVQEKGSRNKRKYRADPPLGDLNKITSSSQDECPSYEFSAEKFEISSSLGQTNACDLC 79
           +++ QEKG +NKRK      L D ++  +SS  E P YE  + K                
Sbjct: 24  TVSPQEKGRKNKRK------LADPSQPNASSLTEFPPYELPSLK---------------- 83

Query: 80  SISQEFSAGLKLDLGLSNGGSSDVGLNWPRGELEVDEFQDADWSDLTEAQLEELVLSNLD 139
              Q   +G        NG   +V       +L+V+  +  +W D     LEEL+ SNL 
Sbjct: 84  --PQNHLSG--------NGSVGEVS-----NQLQVEVSESVEWDDPFACHLEELLSSNLL 143

Query: 140 TIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDTVSNIVDNTLAFLRSGQEIDHSREH 199
           T+F   +K+++  GYT++  +KAVSR  +  G  + +SNIV+NTL+ L++G E   S ++
Sbjct: 144 TLFLDTMKQLIDLGYTDDEVLKAVSRCRLYCGGNNLLSNIVNNTLSALKTGDEGAGSGDY 203

Query: 200 YFEDLQQLEKYILAELVCVLREVRPFFSTGDAMWCLLICDMNVSHACAMDSDPFNAFVCD 259
            FEDLQQL  Y L E++ +++EVRP  ST +AMW LL+CD+NV  A   +         D
Sbjct: 204 VFEDLQQLVSYTLVEMISLIKEVRPSLSTVEAMWRLLMCDLNVLQAFEAEG--------D 263

Query: 260 GALNESSSNSIPQLKAEAKSSEMSLPKPVKPVSPISCAHSSQSDGPAALGVPNLTKPKEP 319
           G ++ S  +    L AE+   + S P   KP  P S   S++++       PN    K+ 
Sbjct: 264 GLVSSSKLSDSESLGAESNPPKSSDPDNPKP--PQSDPQSNRNEPLKFGNFPNTPNSKKT 323

Query: 320 LFSSGPVSDKELQNSTSDVAEESFSVAGNSQTSVSEEKIGSSRKVHSNITKREY-MLRQK 379
             SSG    KE+ + ++   +    +   S T VS+EK+ S RK     TK+E  MLRQK
Sbjct: 324 Q-SSGTTPGKEVCSGSTVSCQ---GMRSTSFTLVSDEKLVSCRK---GRTKKEIAMLRQK 383

Query: 380 SLHVDKNFRTYGPKGSSRAGKLTGLGGLMLDKKLKSVSGSTAVNFKNASLKISKAMGIDV 439
           S  V+K  RTY      +A K   +G  +L+K++KS S       +N+S KI+  +G+ V
Sbjct: 384 SC-VEK-IRTYSKGSGYKAAKFASVGSFLLEKRVKSSSEFVP---RNSSSKITAEIGVKV 443

Query: 440 --AQDNGSHNLSTIDIPSSSLSFNLENINTVSPFSKTNVLSSLPAPSSPPALPATNISSA 499
             A+D+G        + S  +  + +   T  P       S     S    L     S++
Sbjct: 444 SLAEDSGCFVRKNSKLDSPVVVVDAKGYITALPARSVKSASKKKTGSESVTLIP---SAS 503

Query: 500 LPAADIDLSLSLPTKSNLPS-MPISCNAESSTSSFVEKPYEKSLGQWFPRDKKDEMVLKL 559
              +D  +  +   KS   S    S +A+ +   +   PY+ +LG + PRDKKDE++LKL
Sbjct: 504 EKKSDSSIPSTSEKKSGSESEEKASVSAKLAPDYYAGIPYDAALGIYVPRDKKDELILKL 563

Query: 560 VPRARELQNQLQEWTEWANQKVMQAARRLSKDKAELKTLKQEKEEVERLKKEKQTLEENT 619
           VPR  +LQN+LQ WT+WANQKV +A  RL KD+ ELK L++E+EE E+ KKEKQ LEENT
Sbjct: 564 VPRVNDLQNELQVWTDWANQKVKEATGRLLKDQPELKALRKEREEAEQYKKEKQLLEENT 623

Query: 620 MKKLSEMEHALCKASGQVELANSAVRRLEVENAALRQEMEVAKLRATESAASYQEVSKRE 679
            K+LSEM+ AL  A+ Q+E A +   RLE+E + L++EME AK++A ESA S++E  +R 
Sbjct: 624 RKRLSEMDFALKNATSQLEKAFNTAHRLELEQSILKKEMEAAKIKAVESAESFREAKERG 683

Query: 680 KKTLMKVQSWEKQKMLFQEEHTAEKRKLKQLIQELEQARDLQEQLEMVQKAITALGMESM 739
           +++L  + SWE QK++ QEE   ++ K+  L +E+ +A++ Q Q+E   K          
Sbjct: 684 ERSLKDIHSWEGQKIMLQEELKGQREKVTVLQKEVTKAKNRQNQIEAALKQ--------- 743

Query: 740 VVVSYCTFLLCLVPVDSVAVEHYEFCLKDHSEKLQKVLLITWRIVGPFSLTRNISQGRWK 799
                                                                       
Sbjct: 744 ------------------------------------------------------------ 803

Query: 800 LEERAKDELLMQAASLRKEREQIEASVKAKEDTIKLKAENNLLKYKDDIQKLEKEISQLR 859
            E  AK +L  QA+ +RKE +++EA  K +E+ IK KAE ++  Y D+I++LE+EIS+L+
Sbjct: 804 -ERTAKGKLSAQASLIRKETKELEALGKVEEERIKGKAETDVKYYIDNIKRLEREISELK 822

Query: 860 LKTDSSRIAALKRGIDGSYASRLTDTRNSTDHKESWTPNVSESMKDLYEYSGTGGVKRER 919
           LK+D SRI ALK+G                          SES     E  G   VKRER
Sbjct: 864 LKSDYSRIIALKKG-------------------------SSESKATKRESLGMPKVKRER 822

Query: 920 ECVMCLSEEMSVVFLPCAHQVVCTTCNELHEKQGMKDCPSCRSPIQRRIPVRYARS 972
           ECVMCLSEEMSV+FLPCAHQV+C  CN+LHEK+GM DCPSCR  I RRI  R+ARS
Sbjct: 924 ECVMCLSEEMSVIFLPCAHQVLCFKCNQLHEKEGMMDCPSCRGTIHRRIQARFARS 822

BLAST of Spg031971 vs. TAIR 10
Match: AT2G35330.1 (RING/U-box superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 5.6e-72
Identity = 253/867 (29.18%), Postives = 407/867 (46.94%), Query Frame = 0

Query: 117 FQDAD-WSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDT 176
           F+D + W   TE QLE+++L +L+ ++  AI K+V SGY E+VA++AV  +G C+G  D 
Sbjct: 92  FEDNNGWGYCTEEQLEDILLKHLEYLYNEAISKLVGSGYDEDVALRAVLSNGYCYGGMDV 151

Query: 177 VSNIVDNTLAFLRS----GQEIDH--SREHYFEDLQQLEKYILAELVCVLREVRPFFSTG 236
           ++NI+ N+LA+L+S    G  +++    E  F DL+QLE+Y LA +V +L++V+P  S G
Sbjct: 152 MTNILHNSLAYLKSNTGEGSNVNNEDQSETVFTDLRQLEEYSLAGMVYLLQQVKPNLSKG 211

Query: 237 DAMWCLLICDMNVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVK 296
           DAMWCLL+ +++V  A  MD       +      +SS+  +      A S+   +   + 
Sbjct: 212 DAMWCLLMSELHVGRASTMD-------IPSSGKGDSSNVGV----GGASSTVNGVGGAIA 271

Query: 297 PVSPISCAHSSQSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNS 356
           P   +   H     G            K P FS                           
Sbjct: 272 PA--LCRFHGGWGFG----------NGKGPKFS--------------------------- 331

Query: 357 QTSVSEEKIGSSRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLD 416
                    G+   +HS     E +  Q+ +   + F                     L 
Sbjct: 332 ---------GNGFSLHS-----EELTLQREIDCPRRFN--------------------LS 391

Query: 417 KKLKSVSGSTAVNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSP 476
             +KS+       F                              ++    ++E    V  
Sbjct: 392 PSMKSLLRENVAAF------------------------------AAGYRASMEQKKQVQM 451

Query: 477 FSKTNVLSSLPAPSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSS 536
            S+T+                T++S    A          T S     P    +E   SS
Sbjct: 452 QSETS---------------GTSLSCTAAA----------THSEKCEQPHVFGSEECFSS 511

Query: 537 FVEKPYEKSLG---QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSK 596
            +EK  + +L       P + KD+ ++ L+ + ++L+ QL+E  +WA +K MQAA+++S 
Sbjct: 512 VLEKFRDLNLDDNVDSAPEELKDDALIGLLQQVQDLKKQLKERKDWAQKKAMQAAQKVSD 571

Query: 597 DKAELKTLKQEKEEVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVE 656
           + +ELK+L+ E+EE++R+KK KQT E++T+KKLSEME+AL KASGQV+ AN+ VR LE E
Sbjct: 572 ELSELKSLRSEREEIQRVKKGKQTREDSTLKKLSEMENALRKASGQVDKANAVVRALENE 631

Query: 657 NAALRQEMEVAKLRATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQL 716
           +A +R EME +KL A+ES  +  E SK+EKK L K+ +WEKQKM  Q+E TAEK K+K L
Sbjct: 632 SAEIRAEMEASKLSASESLTACMEASKKEKKCLKKLLAWEKQKMKLQDEITAEKEKIKAL 691

Query: 717 IQELEQARDLQEQLEMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHS 776
            + L Q    +++ E                                             
Sbjct: 692 NRALAQITQEEKEYE--------------------------------------------- 738

Query: 777 EKLQKVLLITWRIVGPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKE 836
                                     +W+ E++AK+++L Q    ++ +E IEAS K K 
Sbjct: 752 -------------------------AKWRQEQKAKEQVLAQVEEEQRSKEAIEASNKRKV 738

Query: 837 DTIKLKAENNLLKYKDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTD 896
           ++++LK E +  ++KDD+Q+LE+E+S  RL   SS  ++L+       ++  + T+  +D
Sbjct: 812 ESLRLKIEIDFQRHKDDLQRLEQELS--RLNKASSTDSSLQ-------SNNTSHTKVKSD 738

Query: 897 HKESWTPNVSESMKDLYEYSGT--GGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNEL 956
             +  T  +S+ +++L    G+       +REC++C+ +E+SVVFLPCAHQVVC +C++ 
Sbjct: 872 KSKGET--MSKLLEELNRLDGSYEKEANYDRECLICMKDEVSVVFLPCAHQVVCASCSDS 738

Query: 957 HEKQGMKDCPSCRSPIQRRIPVRYARS 972
               G   CP CR+P+Q+RI V  A S
Sbjct: 932 FMGSGKATCPCCRAPVQQRIRVFGASS 738

BLAST of Spg031971 vs. TAIR 10
Match: AT2G35330.2 (RING/U-box superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 5.6e-72
Identity = 253/867 (29.18%), Postives = 407/867 (46.94%), Query Frame = 0

Query: 117 FQDAD-WSDLTEAQLEELVLSNLDTIFKSAIKKIVASGYTEEVAIKAVSRSGICFGCKDT 176
           F+D + W   TE QLE+++L +L+ ++  AI K+V SGY E+VA++AV  +G C+G  D 
Sbjct: 65  FEDNNGWGYCTEEQLEDILLKHLEYLYNEAISKLVGSGYDEDVALRAVLSNGYCYGGMDV 124

Query: 177 VSNIVDNTLAFLRS----GQEIDH--SREHYFEDLQQLEKYILAELVCVLREVRPFFSTG 236
           ++NI+ N+LA+L+S    G  +++    E  F DL+QLE+Y LA +V +L++V+P  S G
Sbjct: 125 MTNILHNSLAYLKSNTGEGSNVNNEDQSETVFTDLRQLEEYSLAGMVYLLQQVKPNLSKG 184

Query: 237 DAMWCLLICDMNVSHACAMDSDPFNAFVCDGALNESSSNSIPQLKAEAKSSEMSLPKPVK 296
           DAMWCLL+ +++V  A  MD       +      +SS+  +      A S+   +   + 
Sbjct: 185 DAMWCLLMSELHVGRASTMD-------IPSSGKGDSSNVGV----GGASSTVNGVGGAIA 244

Query: 297 PVSPISCAHSSQSDGPAALGVPNLTKPKEPLFSSGPVSDKELQNSTSDVAEESFSVAGNS 356
           P   +   H     G            K P FS                           
Sbjct: 245 PA--LCRFHGGWGFG----------NGKGPKFS--------------------------- 304

Query: 357 QTSVSEEKIGSSRKVHSNITKREYMLRQKSLHVDKNFRTYGPKGSSRAGKLTGLGGLMLD 416
                    G+   +HS     E +  Q+ +   + F                     L 
Sbjct: 305 ---------GNGFSLHS-----EELTLQREIDCPRRFN--------------------LS 364

Query: 417 KKLKSVSGSTAVNFKNASLKISKAMGIDVAQDNGSHNLSTIDIPSSSLSFNLENINTVSP 476
             +KS+       F                              ++    ++E    V  
Sbjct: 365 PSMKSLLRENVAAF------------------------------AAGYRASMEQKKQVQM 424

Query: 477 FSKTNVLSSLPAPSSPPALPATNISSALPAADIDLSLSLPTKSNLPSMPISCNAESSTSS 536
            S+T+                T++S    A          T S     P    +E   SS
Sbjct: 425 QSETS---------------GTSLSCTAAA----------THSEKCEQPHVFGSEECFSS 484

Query: 537 FVEKPYEKSLG---QWFPRDKKDEMVLKLVPRARELQNQLQEWTEWANQKVMQAARRLSK 596
            +EK  + +L       P + KD+ ++ L+ + ++L+ QL+E  +WA +K MQAA+++S 
Sbjct: 485 VLEKFRDLNLDDNVDSAPEELKDDALIGLLQQVQDLKKQLKERKDWAQKKAMQAAQKVSD 544

Query: 597 DKAELKTLKQEKEEVERLKKEKQTLEENTMKKLSEMEHALCKASGQVELANSAVRRLEVE 656
           + +ELK+L+ E+EE++R+KK KQT E++T+KKLSEME+AL KASGQV+ AN+ VR LE E
Sbjct: 545 ELSELKSLRSEREEIQRVKKGKQTREDSTLKKLSEMENALRKASGQVDKANAVVRALENE 604

Query: 657 NAALRQEMEVAKLRATESAASYQEVSKREKKTLMKVQSWEKQKMLFQEEHTAEKRKLKQL 716
           +A +R EME +KL A+ES  +  E SK+EKK L K+ +WEKQKM  Q+E TAEK K+K L
Sbjct: 605 SAEIRAEMEASKLSASESLTACMEASKKEKKCLKKLLAWEKQKMKLQDEITAEKEKIKAL 664

Query: 717 IQELEQARDLQEQLEMVQKAITALGMESMVVVSYCTFLLCLVPVDSVAVEHYEFCLKDHS 776
            + L Q    +++ E                                             
Sbjct: 665 NRALAQITQEEKEYE--------------------------------------------- 711

Query: 777 EKLQKVLLITWRIVGPFSLTRNISQGRWKLEERAKDELLMQAASLRKEREQIEASVKAKE 836
                                     +W+ E++AK+++L Q    ++ +E IEAS K K 
Sbjct: 725 -------------------------AKWRQEQKAKEQVLAQVEEEQRSKEAIEASNKRKV 711

Query: 837 DTIKLKAENNLLKYKDDIQKLEKEISQLRLKTDSSRIAALKRGIDGSYASRLTDTRNSTD 896
           ++++LK E +  ++KDD+Q+LE+E+S  RL   SS  ++L+       ++  + T+  +D
Sbjct: 785 ESLRLKIEIDFQRHKDDLQRLEQELS--RLNKASSTDSSLQ-------SNNTSHTKVKSD 711

Query: 897 HKESWTPNVSESMKDLYEYSGT--GGVKRERECVMCLSEEMSVVFLPCAHQVVCTTCNEL 956
             +  T  +S+ +++L    G+       +REC++C+ +E+SVVFLPCAHQVVC +C++ 
Sbjct: 845 KSKGET--MSKLLEELNRLDGSYEKEANYDRECLICMKDEVSVVFLPCAHQVVCASCSDS 711

Query: 957 HEKQGMKDCPSCRSPIQRRIPVRYARS 972
               G   CP CR+P+Q+RI V  A S
Sbjct: 905 FMGSGKATCPCCRAPVQQRIRVFGASS 711

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0049418.10.0e+0085.07putative E3 ubiquitin-protein ligase RF298 [Cucumis melo var. makuwa] >TYK16096.... [more]
XP_008438753.10.0e+0085.07PREDICTED: putative E3 ubiquitin-protein ligase RF298 [Cucumis melo][more]
XP_004134170.20.0e+0084.96putative E3 ubiquitin-protein ligase RF298 [Cucumis sativus] >KGN57044.1 hypothe... [more]
XP_022137773.10.0e+0085.49putative E3 ubiquitin-protein ligase RF298 isoform X1 [Momordica charantia][more]
KAG7028938.10.0e+0084.04putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. arg... [more]
Match NameE-valueIdentityDescription
Q0WPJ76.6e-12637.33Putative E3 ubiquitin-protein ligase RF298 OS=Arabidopsis thaliana OX=3702 GN=RF... [more]
Q9ZVT81.2e-11635.98Putative E3 ubiquitin-protein ligase RF4 OS=Arabidopsis thaliana OX=3702 GN=RF4 ... [more]
Q8RX228.0e-5526.43MND1-interacting protein 1 OS=Arabidopsis thaliana OX=3702 GN=MIP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3D0B80.0e+0085.07Putative E3 ubiquitin-protein ligase RF298 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3AX620.0e+0085.07putative E3 ubiquitin-protein ligase RF298 OS=Cucumis melo OX=3656 GN=LOC1034837... [more]
A0A0A0L7S30.0e+0084.96RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G150240 P... [more]
A0A6J1CBB50.0e+0085.49putative E3 ubiquitin-protein ligase RF298 isoform X1 OS=Momordica charantia OX=... [more]
A0A6J1GLE60.0e+0083.83putative E3 ubiquitin-protein ligase RF298 isoform X1 OS=Cucurbita moschata OX=3... [more]
Match NameE-valueIdentityDescription
AT4G03000.14.7e-12737.33RING/U-box superfamily protein [more]
AT4G03000.24.7e-12737.33RING/U-box superfamily protein [more]
AT1G03365.18.9e-11835.98RING/U-box superfamily protein [more]
AT2G35330.15.6e-7229.18RING/U-box superfamily protein [more]
AT2G35330.25.6e-7229.18RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 801..828
NoneNo IPR availableCOILSCoilCoilcoord: 633..660
NoneNo IPR availableCOILSCoilCoilcoord: 577..621
NoneNo IPR availableCOILSCoilCoilcoord: 830..864
NoneNo IPR availableCOILSCoilCoilcoord: 693..733
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 914..962
e-value: 1.1E-11
score: 44.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..51
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 326..357
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 272..363
NoneNo IPR availablePANTHERPTHR46405:SF2OS05G0141500 PROTEINcoord: 14..725
NoneNo IPR availablePANTHERPTHR46405:SF2OS05G0141500 PROTEINcoord: 789..970
NoneNo IPR availablePANTHERPTHR46405OS05G0141500 PROTEINcoord: 789..970
NoneNo IPR availablePANTHERPTHR46405OS05G0141500 PROTEINcoord: 14..725
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 916..964
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 896..967
e-value: 2.0E-13
score: 51.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 917..957
score: 9.89275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031971.1Spg031971.1mRNA