Spg031911 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031911
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Werner Syndrome-like exonuclease
Location	scaffold11: 41734404 .. 41738983 (-)
RNA-Seq Expression	Spg031911
Synteny	Spg031911

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGTTCCATTCAGCTGAAAATGGAATTCCCCTACAGTAGCAACGGCGTCGGAGCTTCGACTTCGACGAAGCTCTCCGTCTGTTCCTCATCTTCTTCGTCGTGTTCGTTCTCCATATCGGAGTTTTCGGAGTTCGATCAGCCTTTTACAGAGCAACAACTCCAAGCAATCGAGGCCATTGAAGCTGCATATCAATCTACCAGCGCTAAAAGACGACGAGCAAGTTCAACAGCAGACGAAAATGAGGTTTCGTGCGTTTCTCCCAACTCCGGCCGTCGATTGCCTCATTCCATTTTGTCTCTCCAGTCGCCATATTCTTCTCCATTATCGCCCTGTCGAGGTAATTTTTTGAAATTTTGTTTCTCCCGTGTTTCGAACTTCCGTCTCCGGCGAACAAACTTCCGTCCCCGGCGTTTCAACTCCGACATTGACGATGGAAGAGAAAATTGTTGTGTTTTTTTTTGCTCTCAGATCTGAAAAAAGGAGGTTTCTCCTGTGTGGGTTTCTTTTCTGAAATTTGGTTTGCTGCGTTTTTGTTGATAAAGAAGAAGATGGGAGGGTTGGAGAGGGTTTATTGAGGAAGAAGAAGATGAGGGGAGTAGGGATGGGTTTGTTGAGGAAGAAGATTGGAGGGGTGAGGAAGGAGATGTGGATTCGGGGGGTGGGGAAGGGGAAAGGGAGGGAAGAGGAAGAAGGGGATTCGTTTGGAGAGGGGTTGCGGCGGCGACAAAGGGTTGCGGAAAGGGGTGGCGGCGGCGGCAAAGGTTAGATTTAGGTGGAGGGCGAGTGGGTTCGTTTGGAAGAAGGGAGGAGGCTCCGGCGAATTGGCGGCGACGGCGGGGGGAGGAGAAGGGAGGAGCTCCGATGCAGTGGGGAAGTGGCGGCGGCGGCGATAGGGGAGGAGGCGGCGACGGCGACGGTGAAGAAGAAGAAAATAGGGAGAGAAGCCGCCGTCGCCGGCAAGGAAAGGGAGGGGGAAAGGAGAGAGAAAGAAAGGGAAACAAAGGGAAAGGGGAAGAAAGGGGGTTCGGGAAAGGGGGAGGGTGGGGGAGAGAGAAAGAGTAGTTTTTTTTTTTTTTTAAATAATTATTATTATTTTAATATATTGATATATTATTATTTTAATTAAAATTAATATTTTAATATTTTAAAATATGCCACGTCATCACCGTTAAAAAAGGGACTCAATAGAATCATTTTTTAAACAGTAAGGACTTGATTGTTCAATTTTCAAACCTAGGGACCTATTGGAAACAACCTGAAACATGAGCGACCAAAATGGTATTTTACCCTAGAAGAAAAAAAAAAAAAAAAAACTAACCCATAAAACTCAGATTTTAGGGGTTTTTTTGAGCTAAAATTGAAATTCTGAGTGCGTTCTTTACTGTTTGCGTTTGCAGTAAACTCGAAGATGAGATTTCCTGCATTGAATTTTGGTGGTCGAGTTATTTACAGTAGAACTTCGAGTGAGGTTGATAGAGCGTCCCGGGAGCTAGCAAAGAAAATCGACTTCATGAGGAAAGGGATGGATCAAATTACTATTGGATTCGACATCGAATGGAAACCCACATTTAGAAGAGGTCAGTAACTCGGTTGATTCAATTAAACAATGGATTAAGAAGTTAATCATTCAGAGTGAGAGAGAGAGAGAGTGATTGAGAGCGAGTTCTCAAAGAGCAGAACGTGTCTTTGGCACTTTACTTGTTACCTGTGTAATTCCTCATTGTTTAATCAAATTACTGCCGAGAATTGGATAGTGCATTCTTGATTTCTCGAGTTCTTTGTTCTTTCTATCATTCTCTTTCCCTTCGTGATTGTAGTTTGTTGTTTCATGAACTTAAATCCTGACTAGTTTTATGGCTAACTTTTTTTTTTTTTTGAGTTCAACAACATATTGGGTGGGGATTTGAACCATAATATGGTGAAGGTTATAACATCTTGGCTAGTTGAGTTATACTCGAGTCGAGTTGGTAGCTCATGCTAATTACTCCGTCTATCATTCTTACAAACCTTTTTAATTGGTCATGGATTCCATGATATGTGTTGATCAAAACAAGAATTTCTACTTCTTTGACTGATAAATTCATCTGCAGGTGTTCCACCCGGAAAGGCAGCAGTAATGCAACTGTGTTTGGACAATTCTCAATGCCATGTTATGCACATCATTCATTCGGGAATACCCCAAAGTTTGCAGGCTCTTCTAGAGGATGACACGCTCGGCAAGGTTTGAAACATTGCCATAATTGCTTTTGTAATTTAATTTTCCTGTACTTTCTCTGACCCATTTTGATCAATCATGCTGAAGAACTCCAGGCTGGAGTTGGCATTGTCAATGATGCTATGAAAGTTTTTAAAGATTATAACGTGTCTATCAAAGGAGTGGATGAAATTTCGGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGAGTCTTAGGGCTCTAACTGAGACGCTCATATCAAAAGAGGTAAGTGCTTGTCCTTTTTTCTTTTTTTATTTTTTATTTTTTATTTTTTATTATTTTTTTTTTCCTTATGAGTTAAGCAAGTGTATTTAATAAAAAAAATTATCCATCTGATGTCAGGAAGCTGAAGTTTTCTTTATTATAATATTTATGGCCCTTCAATGACAACTTTTCTTTTTCTTTTTGGTAACTGGGGCATTGAGAGCTCCACCCTTGAGGTATTACCCACCTCAAAGTTAGCTCCACAAAACAGTTTTAAATTATTAAATTTTCTCATTTGGAAAATTTTCAAACATTCCTCCTTCACTACTTAGTTATGCCAGTGTTAGTGATACCAATTTGAGCATAGTTCAATTGGTTAAAGTATATGTCATGGATCAAGAGGTCAGAGATTTGAATCCTTCCATCCCACATGTGGTTGAACTCAAAAATGCATTTATCACTTTTTTATTTCTGTAAGGTTTCTAAAAAAAAGTTTTTGATTTTTGAAATTTATGTTTATTTACTAACAATTTTCCTTATTCTGCAATCTAGTTTTAGAATGTTTCTGAAATCCTAGGTAAACTCTAAAAACTAAATCCATTCTCTCTTCATCATCCACTGCCCCAATGCCATGCCTATACTCAGACAAATTGAGTGAATGTTCGTAGAGATCCTGAGCTCAAGTATATTTGCAATTTGCAAATGAAATTGTCATCTTTATTCAAATTTTATCGAACACTGTAGCTTCCAAAGCCCAACGAAATCAGGCTTGGAAACTGGGAGGTCGCTGAGTTATCAAAGGAGCAACTGAAATATGCTGCCACAGACGCATTTGCTTCTTGGTACTTACATGAGGTATTAACGAAATCAAGCTTTTCTTTTTTGATAATTGATTTAAGATCTCTTGAAGTCTTCATAAAGAAAAAAAATAAATAAATAATCATTAGAATAATCTCAACCTCTAATAATATTCACAGATCTTAAAGGGATTTCCACATGCTCAAAAAGTAGCAGATTCAGAGGGAGATAAGGAGCCAATCTGTTTGATGCCACAATGACATCCAATGCCGAAGTTCAATACGCAGCTAAGTTATCCAATTGATCTAATTAATCTTATTGTTGAAAATTGTTTCTTATCTTAAGAAACTATATTGGTTTGATTAATCTTAAGCTGGCATGCCATTTCAATTTTATGTCGTGTAGTATAATGTTATTTTTAATTGAGAAAAAAAAAAAACTTGAAACACAATTATCATTGAGGAACGAAAGTAGAATTTACGAGGCTAATGTGAACATATGGAAATGTCAACATGTCGATGGAAATTTAATACCTACACATGAAGACCAACTCCAAGCTCCTCCTAATTTGAATTAATTATAATGAGAATATAGTTTCTTAACTCTAGTATCCGAAACCTAAAGATATTGGTAAAGATACATAGTTCAGTAAGGGTAAAGCTAAAAAATTTTACGTTCTAACTTTTTATATTATAAATATGATTTTGGAAATTTAGAACTTTTTTATATAAATTTTTCTATTTAAGACAAACTGTGGAGGTACCTCCTTCCTTCTTTGCTCCATCCTTGAAGTTTAGTTAACCGAGCACTTTTCTATTATGATCTTTGCTGGGAAACTTTGATTTCGCTTTTCAACCAAATGCTTCTTAAAGTTGATTTCAAAGCATGTGTGCATAGCTCCTTGGCTTTCTCTCGAACAAGCGACTACGAAAAGCATTCGAAGAATCTAAAAGGACAGAAAACTGTAGAAGAGAGCACCAAACTTTCTTATTAAAGAAGCAATGAGAAATAGATGATCCAAAGCTTTCCAACCAAAGAGGATCAACACAAAGAAAGGAGCATGAGACATCTTTGTCTAAAAAGTTGACTATGTTTGTAGGTTATTACAAGTCCATTTCTATTCAAAAAGATGGCTTGTAGTCTCTTCCAAACATTGGGGACACAAGCTTCTTGGCTTTCCTATGTTGTGTTGTGTAATGTTGTAAAAAGCATCCTCCATTTTTAGTGCAATGTCTCTATTGTTGGTTTAGATCAATTTTTGAAAGACTAGACTCAACGAGTAAACATCAACGTAACTCGACGATGATTGACATTATGGTCAAAGGT

mRNA sequence

ATGGAATTCCCCTACAGTAGCAACGGCGTCGGAGCTTCGACTTCGACGAAGCTCTCCGTCTGTTCCTCATCTTCTTCGTCGTGTTCGTTCTCCATATCGGAGTTTTCGGAGTTCGATCAGCCTTTTACAGAGCAACAACTCCAAGCAATCGAGGCCATTGAAGCTGCATATCAATCTACCAGCGCTAAAAGACGACGAGCAAGTTCAACAGCAGACGAAAATGAGGTTTCGTGCGTTTCTCCCAACTCCGGCCGTCGATTGCCTCATTCCATTTTGTCTCTCCAGTCGCCATATTCTTCTCCATTATCGCCCTGTCGAGAAGAAGATGGGAGGGTTGGAGAGGGTTTATTGAGGAAGAAGAAGATGAGGGGAGTAGGGATGGGTTTGTTGAGGAAGAAGATTGGAGGGGTGAGGAAGGAGATGTGGATTCGGGGGGTGGGGAAGGGGAAAGGGAGGGAAGAGGAAGAAGGGGATTCGTTTGGAGAGGGGTTGCGGCGGCGACAAAGGGTTGCGGAAAGGGGTGGCGGCGGCGGCAAAGTAAACTCGAAGATGAGATTTCCTGCATTGAATTTTGGTGGTCGAGTTATTTACAGTAGAACTTCGAGTGAGGTTGATAGAGCGTCCCGGGAGCTAGCAAAGAAAATCGACTTCATGAGGAAAGGGATGGATCAAATTACTATTGGATTCGACATCGAATGGAAACCCACATTTAGAAGAGGTGTTCCACCCGGAAAGGCAGCAGTAATGCAACTGTGTTTGGACAATTCTCAATGCCATGTTATGCACATCATTCATTCGGGAATACCCCAAAGTTTGCAGGCTCTTCTAGAGGATGACACGCTCGGCAAGGCTGGAGTTGGCATTGTCAATGATGCTATGAAAGTTTTTAAAGATTATAACGTGTCTATCAAAGGAGTGGATGAAATTTCGGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGAGTCTTAGGGCTCTAACTGAGACGCTCATATCAAAAGAGCTTCCAAAGCCCAACGAAATCAGGCTTGGAAACTGGGAGGTCGCTGAGTTATCAAAGGAGCAACTGAAATATGCTGCCACAGACGCATTTGCTTCTTGGTACTTACATGAGATCTTAAAGGGATTTCCACATGCTCAAAAAGTAGCAGATTCAGAGGGAGATAAGGAGCCAATCTGTTTGATGCCACAATGA

Coding sequence (CDS)

ATGGAATTCCCCTACAGTAGCAACGGCGTCGGAGCTTCGACTTCGACGAAGCTCTCCGTCTGTTCCTCATCTTCTTCGTCGTGTTCGTTCTCCATATCGGAGTTTTCGGAGTTCGATCAGCCTTTTACAGAGCAACAACTCCAAGCAATCGAGGCCATTGAAGCTGCATATCAATCTACCAGCGCTAAAAGACGACGAGCAAGTTCAACAGCAGACGAAAATGAGGTTTCGTGCGTTTCTCCCAACTCCGGCCGTCGATTGCCTCATTCCATTTTGTCTCTCCAGTCGCCATATTCTTCTCCATTATCGCCCTGTCGAGAAGAAGATGGGAGGGTTGGAGAGGGTTTATTGAGGAAGAAGAAGATGAGGGGAGTAGGGATGGGTTTGTTGAGGAAGAAGATTGGAGGGGTGAGGAAGGAGATGTGGATTCGGGGGGTGGGGAAGGGGAAAGGGAGGGAAGAGGAAGAAGGGGATTCGTTTGGAGAGGGGTTGCGGCGGCGACAAAGGGTTGCGGAAAGGGGTGGCGGCGGCGGCAAAGTAAACTCGAAGATGAGATTTCCTGCATTGAATTTTGGTGGTCGAGTTATTTACAGTAGAACTTCGAGTGAGGTTGATAGAGCGTCCCGGGAGCTAGCAAAGAAAATCGACTTCATGAGGAAAGGGATGGATCAAATTACTATTGGATTCGACATCGAATGGAAACCCACATTTAGAAGAGGTGTTCCACCCGGAAAGGCAGCAGTAATGCAACTGTGTTTGGACAATTCTCAATGCCATGTTATGCACATCATTCATTCGGGAATACCCCAAAGTTTGCAGGCTCTTCTAGAGGATGACACGCTCGGCAAGGCTGGAGTTGGCATTGTCAATGATGCTATGAAAGTTTTTAAAGATTATAACGTGTCTATCAAAGGAGTGGATGAAATTTCGGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGAGTCTTAGGGCTCTAACTGAGACGCTCATATCAAAAGAGCTTCCAAAGCCCAACGAAATCAGGCTTGGAAACTGGGAGGTCGCTGAGTTATCAAAGGAGCAACTGAAATATGCTGCCACAGACGCATTTGCTTCTTGGTACTTACATGAGATCTTAAAGGGATTTCCACATGCTCAAAAAGTAGCAGATTCAGAGGGAGATAAGGAGCCAATCTGTTTGATGCCACAATGA

Protein sequence

MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQSTSAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKKKMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKVNSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKGVDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLKYAATDAFASWYLHEILKGFPHAQKVADSEGDKEPICLMPQ

Homology

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BLAST of Spg031911 vs. NCBI nr
Match: KAG7028742.1 (Werner Syndrome-like exonuclease [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501.9 bits (1291), Expect = 5.1e-138
Identity = 278/400 (69.50%), Postives = 296/400 (74.00%), Query Frame = 0


Query: 1   MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQST 60
           MEFP SSN  GA  ST+LSVCSS SSS SFS+   SEFDQPFTEQQLQAIEAIEAAYQ+T
Sbjct: 1   MEFPCSSNRAGA--STELSVCSSPSSSYSFSL---SEFDQPFTEQQLQAIEAIEAAYQAT 60

Query: 61  SAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKK 120
           +AKRRR      ENEVSCVSP+SGRRLP SI SLQSP+SSPLSPCR              
Sbjct: 61  TAKRRR------ENEVSCVSPDSGRRLPRSISSLQSPHSSPLSPCR-------------- 120

Query: 121 KMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKV 180
                                                                      V
Sbjct: 121 -----------------------------------------------------------V 180

Query: 181 NSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRG 240
            SKMRFPAL F GR++YSRT+SEVDRASRELAKKI+ MRKGMDQITIGFDIEWKPTFRRG
Sbjct: 181 TSKMRFPALKFDGRIVYSRTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRG 240

Query: 241 VPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYN 300
           VPPGKAAVMQLCLD+SQCHVMHIIHSGIP+SLQALLEDDTL KAGVGIVNDAMKVFKDYN
Sbjct: 241 VPPGKAAVMQLCLDHSQCHVMHIIHSGIPRSLQALLEDDTLSKAGVGIVNDAMKVFKDYN 300

Query: 301 VSIKGVDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLK 360
           VSIK VDEIS+LANQKLAGVPKKWSL +LTE LISKELPKP++IRLGNWEVA LSKEQL 
Sbjct: 301 VSIKAVDEISNLANQKLAGVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLH 316

Query: 361 YAATDAFASWYLHEILKGFPHAQKVADSEGDKEPICLMPQ 401
           YAATDAFASWYLHEILKGFP  QK ADSEGD+EPIC+MPQ
Sbjct: 361 YAATDAFASWYLHEILKGFPDPQKAADSEGDEEPICVMPQ 316

[Close]

BLAST of Spg031911 vs. NCBI nr
Match: XP_022947132.1 (Werner Syndrome-like exonuclease [Cucurbita moschata])

HSP 1 Score: 501.9 bits (1291), Expect = 5.1e-138
Identity = 278/400 (69.50%), Postives = 295/400 (73.75%), Query Frame = 0


Query: 1   MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQST 60
           MEFP SSN  GA  ST+LSVCSS SSS SFS+   SEFDQPFTEQQLQAIEAIEAAYQ+T
Sbjct: 1   MEFPCSSNRAGA--STELSVCSSPSSSYSFSL---SEFDQPFTEQQLQAIEAIEAAYQAT 60

Query: 61  SAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKK 120
           +AKRRR      ENEV CVSPNSGRRLP SI SLQSP+SSPLSPCR              
Sbjct: 61  TAKRRR------ENEVLCVSPNSGRRLPRSISSLQSPHSSPLSPCR-------------- 120

Query: 121 KMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKV 180
                                                                      V
Sbjct: 121 -----------------------------------------------------------V 180

Query: 181 NSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRG 240
            SKMRFPAL FGGR++YSRT+SEVDRASRELAKKI+ MRKGMDQITIGFDIEWKPTFRRG
Sbjct: 181 TSKMRFPALKFGGRIVYSRTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRG 240

Query: 241 VPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYN 300
           VPPGKAAVMQLCLD+SQCHVMHIIHSGIP+SLQALLEDDTL KAGVGIVNDAMKVFKDYN
Sbjct: 241 VPPGKAAVMQLCLDHSQCHVMHIIHSGIPRSLQALLEDDTLNKAGVGIVNDAMKVFKDYN 300

Query: 301 VSIKGVDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLK 360
           VSIK VDEIS+LANQKLAGVPKKWSL +LTE LISKELPKP++IRLGNWEVA LSKEQL 
Sbjct: 301 VSIKAVDEISNLANQKLAGVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLH 316

Query: 361 YAATDAFASWYLHEILKGFPHAQKVADSEGDKEPICLMPQ 401
           YAATDAFASWYLHEILKGFP  Q  ADSEGD+EPIC+MPQ
Sbjct: 361 YAATDAFASWYLHEILKGFPDPQAAADSEGDEEPICVMPQ 316

[Close]

BLAST of Spg031911 vs. NCBI nr
Match: KAG6597271.1 (Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 501.5 bits (1290), Expect = 6.6e-138
Identity = 277/400 (69.25%), Postives = 296/400 (74.00%), Query Frame = 0


Query: 1   MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQST 60
           MEFP SSN  GA  ST+LSVCSS SSS SFS+   SEFDQPFTEQQLQAIEAIEAAYQ+T
Sbjct: 1   MEFPCSSNRAGA--STELSVCSSPSSSYSFSL---SEFDQPFTEQQLQAIEAIEAAYQAT 60

Query: 61  SAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKK 120
           +AKRRR      ENEVSCVSP+SGRRLP S+ SLQSP+SSPLSPCR              
Sbjct: 61  TAKRRR------ENEVSCVSPDSGRRLPRSVSSLQSPHSSPLSPCR-------------- 120

Query: 121 KMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKV 180
                                                                      V
Sbjct: 121 -----------------------------------------------------------V 180

Query: 181 NSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRG 240
            SKMRFPAL F GR++YSRT+SEVDRASRELAKKI+ MRKGMDQITIGFDIEWKPTFRRG
Sbjct: 181 TSKMRFPALKFDGRIVYSRTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRG 240

Query: 241 VPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYN 300
           VPPGKAAVMQLCLD+SQCHVMHIIHSGIP+SLQALLEDDTL KAGVGIVNDAMKVFKDYN
Sbjct: 241 VPPGKAAVMQLCLDHSQCHVMHIIHSGIPRSLQALLEDDTLSKAGVGIVNDAMKVFKDYN 300

Query: 301 VSIKGVDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLK 360
           VSIK VDEIS+LANQKLAGVPKKWSL +LTE LISKELPKP++IRLGNWEVA LSKEQL 
Sbjct: 301 VSIKAVDEISNLANQKLAGVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLH 316

Query: 361 YAATDAFASWYLHEILKGFPHAQKVADSEGDKEPICLMPQ 401
           YAATDAFASWYLHEILKGFP  QK ADSEGD+EPIC+MPQ
Sbjct: 361 YAATDAFASWYLHEILKGFPDPQKAADSEGDEEPICVMPQ 316

[Close]

BLAST of Spg031911 vs. NCBI nr
Match: XP_023540073.1 (Werner Syndrome-like exonuclease [Cucurbita pepo subsp. pepo])

HSP 1 Score: 497.3 bits (1279), Expect = 1.3e-136
Identity = 276/400 (69.00%), Postives = 295/400 (73.75%), Query Frame = 0


Query: 1   MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQST 60
           MEF  SSN  GA  ST+LSVCSS SSS SFS+   SEFDQPFTEQQLQAIEAIEAAYQST
Sbjct: 1   MEFSCSSNRAGA--STELSVCSSPSSSYSFSL---SEFDQPFTEQQLQAIEAIEAAYQST 60

Query: 61  SAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKK 120
           +AKRRR      ENEV CVSPNSGRRLP SI SLQSP+SSPLSPCR              
Sbjct: 61  TAKRRR------ENEVLCVSPNSGRRLPRSISSLQSPHSSPLSPCR-------------- 120

Query: 121 KMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKV 180
                                                                      V
Sbjct: 121 -----------------------------------------------------------V 180

Query: 181 NSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRG 240
            SKMRFPAL FGGR++YSRT+SEVDRASRELAKKI+ MRKGMDQITIGFDIEWKPTFRRG
Sbjct: 181 TSKMRFPALKFGGRIVYSRTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRG 240

Query: 241 VPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYN 300
           VPPGKAAVMQLCLD+SQCHVMHIIHSGIP+SLQALLEDDTL KAGVGIVNDAMKVFKD+N
Sbjct: 241 VPPGKAAVMQLCLDHSQCHVMHIIHSGIPRSLQALLEDDTLSKAGVGIVNDAMKVFKDHN 300

Query: 301 VSIKGVDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLK 360
           VSIK VDEIS+LAN+KLAGVPKKWSL +LTE LISKELPKP++IRLGNWEVA LSKEQL 
Sbjct: 301 VSIKAVDEISNLANRKLAGVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLH 316

Query: 361 YAATDAFASWYLHEILKGFPHAQKVADSEGDKEPICLMPQ 401
           YAATDAFASWYLHEILKGFP  Q+ ADSEGD+EPIC+MPQ
Sbjct: 361 YAATDAFASWYLHEILKGFPDPQEAADSEGDEEPICVMPQ 316

[Close]

BLAST of Spg031911 vs. NCBI nr
Match: XP_022974736.1 (Werner Syndrome-like exonuclease [Cucurbita maxima])

HSP 1 Score: 492.7 bits (1267), Expect = 3.1e-135
Identity = 275/401 (68.58%), Postives = 294/401 (73.32%), Query Frame = 0


Query: 1   MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQST 60
           MEFP SSN  GA  ST+LSVCSS SSS SFS+   SEFDQPFTEQQLQAIEAIEA YQ+T
Sbjct: 1   MEFPCSSNRAGA--STELSVCSSPSSSYSFSV---SEFDQPFTEQQLQAIEAIEAEYQAT 60

Query: 61  SAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKK 120
           +AKRRR      ENEV CVSPNSGRRLP SI SLQSP+SSPLSPCR              
Sbjct: 61  TAKRRR------ENEVLCVSPNSGRRLPRSISSLQSPHSSPLSPCR-------------- 120

Query: 121 KMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKV 180
                                                                      V
Sbjct: 121 -----------------------------------------------------------V 180

Query: 181 NSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRG 240
            S MRFPAL FGGR++YSRT+SEVDRASRELAKKI+ MRKGMDQITIGFDIEWKPTFRRG
Sbjct: 181 TSMMRFPALKFGGRIVYSRTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRG 240

Query: 241 VPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYN 300
           VPPGKAAVMQLCLD+SQCHVMHIIHSGIP+SLQALLED+TL KAGVGIVNDAMKVFKDYN
Sbjct: 241 VPPGKAAVMQLCLDHSQCHVMHIIHSGIPRSLQALLEDNTLSKAGVGIVNDAMKVFKDYN 300

Query: 301 VSIKGVDEISDLANQKL-AGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQL 360
           VSI+ VDEIS+LANQKL AGVPKKWSL +LTE LISKELPKP++IRLGNWEVA LSKEQL
Sbjct: 301 VSIRAVDEISNLANQKLAAGVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAMLSKEQL 317

Query: 361 KYAATDAFASWYLHEILKGFPHAQKVADSEGDKEPICLMPQ 401
            YAATDAFASWYLHEILKGFP  QK ADSEGD+EPIC+MPQ
Sbjct: 361 HYAATDAFASWYLHEILKGFPDPQKAADSEGDEEPICVMPQ 317

[Close]

BLAST of Spg031911 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.0e-64
Identity = 146/361 (40.44%), Postives = 200/361 (55.40%), Query Frame = 0


Query: 32  ISEFSEFDQPFTEQQLQAIEAIEAAY--------QSTSAKRRRASST--ADENEVSCVSP 91
           +S  +  D  FTE++L AI+AIEA+Y         S++A   +A+++    E + + +  
Sbjct: 1   MSSSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPN 60

Query: 92  NSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKKKMRGVGMGLLRKKIGGVRKEM 151
           N  R+LP SI S  S    PLS CR  +                                
Sbjct: 61  NIRRQLPRSITSSTSYKRFPLSRCRARN-------------------------------- 120

Query: 152 WIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKVNSKMRFPALNFGGRVIYSRTS 211
                                                       FPA+ FGGR++YS+T+
Sbjct: 121 --------------------------------------------FPAMRFGGRILYSKTA 180

Query: 212 SEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVM 271
           +EVD+ + +L K +D  R       +G DIEW+P+FR+GV PGK A +Q+C+D++ C VM
Sbjct: 181 TEVDKRAMQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVM 240

Query: 272 HIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKGVDEISDLANQKLAGVP 331
           HI HSGIPQSLQ L+ED TL K G+GI  D++K+F DY VSIK V+++SDLANQK+ G  
Sbjct: 241 HIFHSGIPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-D 284

Query: 332 KKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLKYAATDAFASWYLHEILKGFPH 383
           KKW L +LTETL+ KEL KPN IRLGNWE   LSK+QL+YAATDA+ASW+L+++LK  P 
Sbjct: 301 KKWGLASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLPD 284

[Close]

BLAST of Spg031911 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 6.7e-32
Identity = 68/193 (35.23%), Postives = 116/193 (60.10%), Query Frame = 0


Query: 187 PALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRGVPPGKA 246
           P + F G ++YS  S++    S ++   +      +++  +GFDIEW P + +G   GK 
Sbjct: 39  PFMKFNGSIVYSYESNDCSLLSEDIRSSL------LEEDVLGFDIEWPPVYTKG-KTGKV 98

Query: 247 AVMQLCLDNSQCHVMHII-HSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKG 306
           A++Q+C+   +C++ HI   +G P+ L+ LLED+++ K GVGI  D  K+  DY + +KG
Sbjct: 99  ALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVGVGIEGDQWKLMSDYELKLKG 158

Query: 307 VDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLKYAATD 366
             E+S++ANQKL    +KW+   L + L  ++L K    R  NW++  L+++Q  YAATD
Sbjct: 159 FIELSEMANQKLR-CKEKWTFNGLIKHLFKEQLYKRKSYRCSNWDIFLLTEDQKLYAATD 218

Query: 367 AFASWYLHEILKG 379
           A+A   +++ L+G
Sbjct: 219 AYAGLLIYKKLEG 223

[Close]

BLAST of Spg031911 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 137.5 bits (345), Expect = 3.3e-31
Identity = 70/192 (36.46%), Postives = 115/192 (59.90%), Query Frame = 0


Query: 187 PALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRGVPPGKA 246
           P L F G ++YS  +S+    S +++  +       D   +GFD+EW P + RG   GK 
Sbjct: 45  PFLEFTGSIVYSYDASDCSFLSEDISMSLS------DGDVVGFDMEWPPLYNRG-KLGKV 104

Query: 247 AVMQLCLDNSQCHVMHIIHSGI-PQSLQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKG 306
           A++QLC+  S+C++ H+    + PQ L+ LLE+  + KAGVGI  D  K+ +D+++ +K 
Sbjct: 105 ALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKN 164

Query: 307 VDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLKYAATD 366
             E++D+AN+KL    + WSL +L + L+ K+L K   IR  NW    L+++Q  YAATD
Sbjct: 165 FVELTDVANKKLK-CTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATD 224

Query: 367 AFASWYLHEILK 378
           A+A + ++  L+
Sbjct: 225 AYAGFIIYRNLE 228

[Close]

BLAST of Spg031911 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 132.1 bits (331), Expect = 1.4e-29
Identity = 68/191 (35.60%), Postives = 114/191 (59.69%), Query Frame = 0


Query: 187 PALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRGVPPGKA 246
           P L F G ++YS  +S+    S +++ ++       D   +GFD+EW P ++ G    + 
Sbjct: 39  PFLEFPGSIVYSYEASDCSFLSEDISMRLS------DGDVVGFDMEWPPIYKPG-KRSRV 98

Query: 247 AVMQLCLDNSQCHVMHIIHSGI-PQSLQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKG 306
           AV+QLC+  S+C++ HI    + PQ L+ LLE+ ++ KAGVGI  D  K+ +D++V ++ 
Sbjct: 99  AVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAGVGIEGDQWKLLRDFDVKLES 158

Query: 307 VDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLKYAATD 366
             E++D+AN+KL    + WSL  L + ++ K+L K   IR  NW    L+++Q  YAATD
Sbjct: 159 FVELTDVANEKLK-CAETWSLNGLVKHVLGKQLLKDKSIRCSNWSNFPLTEDQKLYAATD 218

Query: 367 AFASWYLHEIL 377
           A+A   +++ L
Sbjct: 219 AYAGLIIYQKL 221

[Close]

BLAST of Spg031911 vs. ExPASy Swiss-Prot
Match: Q8VEG4 (Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus OX=10090 GN=Exd2 PE=1 SV=2)

HSP 1 Score: 74.7 bits (182), Expect = 2.6e-12
Identity = 49/180 (27.22%), Postives = 95/180 (52.78%), Query Frame = 0


Query: 217 FMRKGMDQI-TIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVM---HIIHSG--IPQ 276
           F+++ ++    +G D EW     +  P    +++Q+   +  C ++    +I+ G  +P+
Sbjct: 123 FLKRELEDFPVLGIDCEWVNLEGKASP---LSLLQMASPSGFCALVRLPRLIYGGRTLPR 182

Query: 277 SLQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKGVDEISDLANQKLAGV-PKKWSLRAL 336
           +L  +L D  + K GVG   DA K+ +DY + ++G  ++  LA ++   +     SL++L
Sbjct: 183 TLLDILADGAILKVGVGCSEDANKLLQDYGLIVRGCLDLRYLAMKQGNNILCNGLSLKSL 242

Query: 337 TETLISKELPKPNEIRLGNWEVAELSKEQLKYAATDAFASWYLHEILKGFPHAQKVADSE 390
            ET+++  L K   +R  NW+   L+++Q+ YAA DA  S  L   L G+P ++   + E
Sbjct: 243 AETILNFPLDKSLLLRCSNWDAENLTEDQVTYAARDAQISVALFLHLLGYPFSRDSYEEE 299

[Close]

BLAST of Spg031911 vs. ExPASy TrEMBL
Match: A0A6J1G5L3 (Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451092 PE=4 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 2.5e-138
Identity = 278/400 (69.50%), Postives = 295/400 (73.75%), Query Frame = 0


Query: 1   MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQST 60
           MEFP SSN  GA  ST+LSVCSS SSS SFS+   SEFDQPFTEQQLQAIEAIEAAYQ+T
Sbjct: 1   MEFPCSSNRAGA--STELSVCSSPSSSYSFSL---SEFDQPFTEQQLQAIEAIEAAYQAT 60

Query: 61  SAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKK 120
           +AKRRR      ENEV CVSPNSGRRLP SI SLQSP+SSPLSPCR              
Sbjct: 61  TAKRRR------ENEVLCVSPNSGRRLPRSISSLQSPHSSPLSPCR-------------- 120

Query: 121 KMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKV 180
                                                                      V
Sbjct: 121 -----------------------------------------------------------V 180

Query: 181 NSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRG 240
            SKMRFPAL FGGR++YSRT+SEVDRASRELAKKI+ MRKGMDQITIGFDIEWKPTFRRG
Sbjct: 181 TSKMRFPALKFGGRIVYSRTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRG 240

Query: 241 VPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYN 300
           VPPGKAAVMQLCLD+SQCHVMHIIHSGIP+SLQALLEDDTL KAGVGIVNDAMKVFKDYN
Sbjct: 241 VPPGKAAVMQLCLDHSQCHVMHIIHSGIPRSLQALLEDDTLNKAGVGIVNDAMKVFKDYN 300

Query: 301 VSIKGVDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLK 360
           VSIK VDEIS+LANQKLAGVPKKWSL +LTE LISKELPKP++IRLGNWEVA LSKEQL 
Sbjct: 301 VSIKAVDEISNLANQKLAGVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLH 316

Query: 361 YAATDAFASWYLHEILKGFPHAQKVADSEGDKEPICLMPQ 401
           YAATDAFASWYLHEILKGFP  Q  ADSEGD+EPIC+MPQ
Sbjct: 361 YAATDAFASWYLHEILKGFPDPQAAADSEGDEEPICVMPQ 316

[Close]

BLAST of Spg031911 vs. ExPASy TrEMBL
Match: A0A6J1IC67 (Werner Syndrome-like exonuclease OS=Cucurbita maxima OX=3661 GN=LOC111473470 PE=4 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 1.5e-135
Identity = 275/401 (68.58%), Postives = 294/401 (73.32%), Query Frame = 0


Query: 1   MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQST 60
           MEFP SSN  GA  ST+LSVCSS SSS SFS+   SEFDQPFTEQQLQAIEAIEA YQ+T
Sbjct: 1   MEFPCSSNRAGA--STELSVCSSPSSSYSFSV---SEFDQPFTEQQLQAIEAIEAEYQAT 60

Query: 61  SAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKK 120
           +AKRRR      ENEV CVSPNSGRRLP SI SLQSP+SSPLSPCR              
Sbjct: 61  TAKRRR------ENEVLCVSPNSGRRLPRSISSLQSPHSSPLSPCR-------------- 120

Query: 121 KMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKV 180
                                                                      V
Sbjct: 121 -----------------------------------------------------------V 180

Query: 181 NSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRG 240
            S MRFPAL FGGR++YSRT+SEVDRASRELAKKI+ MRKGMDQITIGFDIEWKPTFRRG
Sbjct: 181 TSMMRFPALKFGGRIVYSRTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRG 240

Query: 241 VPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYN 300
           VPPGKAAVMQLCLD+SQCHVMHIIHSGIP+SLQALLED+TL KAGVGIVNDAMKVFKDYN
Sbjct: 241 VPPGKAAVMQLCLDHSQCHVMHIIHSGIPRSLQALLEDNTLSKAGVGIVNDAMKVFKDYN 300

Query: 301 VSIKGVDEISDLANQKL-AGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQL 360
           VSI+ VDEIS+LANQKL AGVPKKWSL +LTE LISKELPKP++IRLGNWEVA LSKEQL
Sbjct: 301 VSIRAVDEISNLANQKLAAGVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAMLSKEQL 317

Query: 361 KYAATDAFASWYLHEILKGFPHAQKVADSEGDKEPICLMPQ 401
            YAATDAFASWYLHEILKGFP  QK ADSEGD+EPIC+MPQ
Sbjct: 361 HYAATDAFASWYLHEILKGFPDPQKAADSEGDEEPICVMPQ 317

[Close]

BLAST of Spg031911 vs. ExPASy TrEMBL
Match: A0A6J1D021 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016124 PE=4 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 2.7e-129
Identity = 264/395 (66.84%), Postives = 278/395 (70.38%), Query Frame = 0


Query: 1   MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQST 60
           MEFP  S   GA  S  LSV SSS SS SFS+   SEFDQPFTEQQLQ I+AIEA YQST
Sbjct: 1   MEFPGCSRNAGA--SPPLSVSSSSFSSRSFSV---SEFDQPFTEQQLQEIDAIEAMYQST 60

Query: 61  SAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKK 120
           S KRRR SSTADENE SCVSPN+GRRLPHSILSLQ PYSSPLSP R              
Sbjct: 61  SGKRRRGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRR-------------- 120

Query: 121 KMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKV 180
                                                                      V
Sbjct: 121 -----------------------------------------------------------V 180

Query: 181 NSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRG 240
           NSKMRFPALNF GR+IYS T+SEVDRASRELAKKI   RKG DQITIGFDIEWKPTFRRG
Sbjct: 181 NSKMRFPALNFDGRIIYSTTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRG 240

Query: 241 VPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYN 300
           VPPGK AVMQLCLDNSQCHVMHIIHSGI  SL+ LLEDDTL KAGVGI NDA+KVFKDYN
Sbjct: 241 VPPGKTAVMQLCLDNSQCHVMHIIHSGITPSLKTLLEDDTLSKAGVGIANDAVKVFKDYN 300

Query: 301 VSIKGVDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLK 360
           VSIK V EISDLANQKLAGVP+KW L +LT+ L+SKEL KPN+IRLGNWEVA LSK+QL+
Sbjct: 301 VSIKAVSEISDLANQKLAGVPQKWGLGSLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQ 317

Query: 361 YAATDAFASWYLHEILKGFPHAQKVADSEGDKEPI 396
           YAATDAFASWYLHEILKGFPHAQKV DSE D EPI
Sbjct: 361 YAATDAFASWYLHEILKGFPHAQKVEDSERDMEPI 317

[Close]

BLAST of Spg031911 vs. ExPASy TrEMBL
Match: A0A1S3AWQ6 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103483463 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 5.1e-128
Identity = 260/389 (66.84%), Postives = 281/389 (72.24%), Query Frame = 0


Query: 1   MEFPYSSNGVGASTSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQST 60
           MEFP SSN     +ST+LS CSSS  S SFS    SEFDQPFTEQQLQAIEAIEAAYQST
Sbjct: 1   MEFPGSSN--SGHSSTELSACSSSFPSWSFS---SSEFDQPFTEQQLQAIEAIEAAYQST 60

Query: 61  SAKRRRASSTADENEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKK 120
           SAK+RR +ST DEN VSCVSPN+GRRLP SI S QSP+ SPLS CR              
Sbjct: 61  SAKKRRPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCR-------------- 120

Query: 121 KMRGVGMGLLRKKIGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKV 180
                                                                      V
Sbjct: 121 -----------------------------------------------------------V 180

Query: 181 NSKMRFPALNFGGRVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRG 240
           NSKMRFPAL++GGR+IYSRT SEVDRASRELAKKI+  RKGM+QITIGFDIEW+P+F+RG
Sbjct: 181 NSKMRFPALSYGGRIIYSRTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRG 240

Query: 241 VPPGKAAVMQLCLDNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYN 300
           VPPGKAAVMQLCL+NS+CHVMHIIHSGIPQSLQALLEDDTL KAGVGI +DA KVFKDYN
Sbjct: 241 VPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYN 300

Query: 301 VSIKGVDEISDLANQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLK 360
           VS+K +DEISDLANQKLAGVPKKW LRALTETLISKEL KP+ IRLGNWEVA LSKEQL 
Sbjct: 301 VSMKQLDEISDLANQKLAGVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLH 311

Query: 361 YAATDAFASWYLHEILKGFPHAQKVADSE 390
           YAATDAFASWYLHEILKGFPH +KVADSE
Sbjct: 361 YAATDAFASWYLHEILKGFPHVEKVADSE 311

[Close]

BLAST of Spg031911 vs. ExPASy TrEMBL
Match: A0A5A7TZU7 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002920 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 4.8e-126
Identity = 253/376 (67.29%), Postives = 274/376 (72.87%), Query Frame = 0


Query: 14  TSTKLSVCSSSSSSCSFSISEFSEFDQPFTEQQLQAIEAIEAAYQSTSAKRRRASSTADE 73
           +ST+LS CSSS  S SFS    SEFDQPFTEQQLQAIEAIEAAYQSTSAK+RR +ST DE
Sbjct: 37  SSTELSACSSSFPSWSFS---SSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTLDE 96

Query: 74  NEVSCVSPNSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKKKMRGVGMGLLRKK 133
           N VSCVSPN+GRRLP SI S QSP+ SPLS CR                           
Sbjct: 97  NGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCR--------------------------- 156

Query: 134 IGGVRKEMWIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKVNSKMRFPALNFGG 193
                                                         VNSKMRFPAL++GG
Sbjct: 157 ----------------------------------------------VNSKMRFPALSYGG 216

Query: 194 RVIYSRTSSEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCL 253
           R+IYSRT SEVDRASRELAKKI+  RKGM+QITIGFDIEW+P+F+RGVPPGKAAVMQLCL
Sbjct: 217 RIIYSRTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCL 276

Query: 254 DNSQCHVMHIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKGVDEISDLA 313
           +NS+CHVMHIIHSGIPQSLQALLEDDTL KAGVGI +DA KVFKDYNVS+K +DEISDLA
Sbjct: 277 ENSECHVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLA 336

Query: 314 NQKLAGVPKKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLKYAATDAFASWYLH 373
           NQKLAGVPKKW LRALTETLISKEL KP+ IRLGNWEVA LSKEQL YAATDAFASWYLH
Sbjct: 337 NQKLAGVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLH 336

Query: 374 EILKGFPHAQKVADSE 390
           EILKGFPH +KVADSE
Sbjct: 397 EILKGFPHVEKVADSE 336

[Close]

BLAST of Spg031911 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 248.8 bits (634), Expect = 7.2e-66
Identity = 146/361 (40.44%), Postives = 200/361 (55.40%), Query Frame = 0


Query: 32  ISEFSEFDQPFTEQQLQAIEAIEAAY--------QSTSAKRRRASST--ADENEVSCVSP 91
           +S  +  D  FTE++L AI+AIEA+Y         S++A   +A+++    E + + +  
Sbjct: 1   MSSSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPN 60

Query: 92  NSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKKKMRGVGMGLLRKKIGGVRKEM 151
           N  R+LP SI S  S    PLS CR  +                                
Sbjct: 61  NIRRQLPRSITSSTSYKRFPLSRCRARN-------------------------------- 120

Query: 152 WIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKVNSKMRFPALNFGGRVIYSRTS 211
                                                       FPA+ FGGR++YS+T+
Sbjct: 121 --------------------------------------------FPAMRFGGRILYSKTA 180

Query: 212 SEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVM 271
           +EVD+ + +L K +D  R       +G DIEW+P+FR+GV PGK A +Q+C+D++ C VM
Sbjct: 181 TEVDKRAMQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVM 240

Query: 272 HIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKGVDEISDLANQKLAGVP 331
           HI HSGIPQSLQ L+ED TL K G+GI  D++K+F DY VSIK V+++SDLANQK+ G  
Sbjct: 241 HIFHSGIPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-D 284

Query: 332 KKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLKYAATDAFASWYLHEILKGFPH 383
           KKW L +LTETL+ KEL KPN IRLGNWE   LSK+QL+YAATDA+ASW+L+++LK  P 
Sbjct: 301 KKWGLASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLPD 284

[Close]

BLAST of Spg031911 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 241.1 bits (614), Expect = 1.5e-63
Identity = 143/355 (40.28%), Postives = 196/355 (55.21%), Query Frame = 0


Query: 32  ISEFSEFDQPFTEQQLQAIEAIEAAY--------QSTSAKRRRASST--ADENEVSCVSP 91
           +S  +  D  FTE++L AI+AIEA+Y         S++A   +A+++    E + + +  
Sbjct: 1   MSSSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPN 60

Query: 92  NSGRRLPHSILSLQSPYSSPLSPCREEDGRVGEGLLRKKKMRGVGMGLLRKKIGGVRKEM 151
           N  R+LP SI S  S    PLS CR  +                                
Sbjct: 61  NIRRQLPRSITSSTSYKRFPLSRCRARN-------------------------------- 120

Query: 152 WIRGVGKGKGREEEEGDSFGEGLRRRQRVAERGGGGGKVNSKMRFPALNFGGRVIYSRTS 211
                                                       FPA+ FGGR++YS+T+
Sbjct: 121 --------------------------------------------FPAMRFGGRILYSKTA 180

Query: 212 SEVDRASRELAKKIDFMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQCHVM 271
           +EVD+ + +L K +D  R       +G DIEW+P+FR+GV PGK A +Q+C+D++ C VM
Sbjct: 181 TEVDKRAMQLIKVLDTKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVM 240

Query: 272 HIIHSGIPQSLQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKGVDEISDLANQKLAGVP 331
           HI HSGIPQSLQ L+ED TL K G+GI  D++K+F DY VSIK V+++SDLANQK+ G  
Sbjct: 241 HIFHSGIPQSLQHLIEDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-D 278

Query: 332 KKWSLRALTETLISKELPKPNEIRLGNWEVAELSKEQLKYAATDAFASWYLHEIL 377
           KKW L +LTETL+ KEL KPN IRLGNWE   LSK+QL+YAATDA+ASW+L++ L
Sbjct: 301 KKWGLASLTETLVCKELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKDL 278

[Close]

BLAST of Spg031911 vs. TAIR 10
Match: AT4G13885.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 86.3 bits (212), Expect = 6.2e-17
Identity = 48/111 (43.24%), Postives = 59/111 (53.15%), Query Frame = 0


Query: 272 LQALLEDDTLGKAGVGIVNDAMKVFKDYNVSIKGVDEISDLANQKLAGVPKKWSLRALTE 331
           LQ L+ED TL K G+GI  D++K+F DY                                
Sbjct: 186 LQHLIEDSTLVKVGIGIDGDSVKLFHDYG------------------------------- 245

Query: 332 TLISKELPKPNEIRLGNWEVAELSKEQLKYAATDAFASWYLHEILKGFPHA 383
                 L KPN IRLGNWEV  LSK+QL+YAATDA+ASW+LH++LK  P A
Sbjct: 246 ------LLKPNRIRLGNWEVHPLSKQQLQYAATDAYASWHLHQVLKDLPDA 259

The following BLAST results are available for this feature:

BLAST of Spg031911 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028742.1	5.1e-138	69.50	Werner Syndrome-like exonuclease [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022947132.1	5.1e-138	69.50	Werner Syndrome-like exonuclease [Cucurbita moschata]	[more]
KAG6597271.1	6.6e-138	69.25	Werner Syndrome-like exonuclease, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_023540073.1	1.3e-136	69.00	Werner Syndrome-like exonuclease [Cucurbita pepo subsp. pepo]	[more]
XP_022974736.1	3.1e-135	68.58	Werner Syndrome-like exonuclease [Cucurbita maxima]	[more]

BLAST of Spg031911 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	1.0e-64	40.44	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
O93530	6.7e-32	35.23	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]
Q14191	3.3e-31	36.46	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O09053	1.4e-29	35.60	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
Q8VEG4	2.6e-12	27.22	Exonuclease 3'-5' domain-containing protein 2 OS=Mus musculus OX=10090 GN=Exd2 P...	[more]

BLAST of Spg031911 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G5L3	2.5e-138	69.50	Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451092 P...	[more]
A0A6J1IC67	1.5e-135	68.58	Werner Syndrome-like exonuclease OS=Cucurbita maxima OX=3661 GN=LOC111473470 PE=...	[more]
A0A6J1D021	2.7e-129	66.84	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016124 ...	[more]
A0A1S3AWQ6	5.1e-128	66.84	Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103483463 PE=4 SV...	[more]
A0A5A7TZU7	4.8e-126	67.29	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Spg031911 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13870.2	7.2e-66	40.44	Werner syndrome-like exonuclease	[more]
AT4G13870.1	1.5e-63	40.28	Werner syndrome-like exonuclease	[more]
AT4G13885.1	6.2e-17	43.24	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 195..381 e-value: 1.5E-16 score: 71.0
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 207..377 e-value: 4.7E-14 score: 52.5
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 178..379 e-value: 1.5E-52 score: 179.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..28
None	No IPR available	PANTHER	PTHR13620:SF45	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 179..384
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 179..384
None	No IPR available	CDD	cd06141	WRN_exo	coord: 199..377 e-value: 2.40707E-57 score: 183.549
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 223..377

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031911.1	Spg031911.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding