Spg031830 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031830
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionCytochrome P450
Locationscaffold11: 42827593 .. 42832215 (-)
RNA-Seq ExpressionSpg031830
SyntenySpg031830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAACATATTCGTTAAACAACCTAATTGATACAACTTTAATAAAACAAAAAAACAAATTAAATAAAATTTCCACGTGAATATTTCTTGTTAAAGATAACAACGACAGGATGACGACAAATTATTATTAAAAAAAAAAAAAAAAAAAAACCCAAAAGTCAAATGATCGTACTCTCGACTTCTGAATCCATCTTCTTCAGCCAAAAAATCCCCAAAAATTTCTCAAAGAATATCTCCCGCTTCGTGATTCTCTCATCCAATCATTGCGATTCAGCCATGGAAGTGAATTTCAACATCTTCACCGTCGTTTCCATCGCACTACTCACAGTCTCCCTCTTCCTTCTGTCCTTGTTCATTCTCCTCCTCCGAACCCTCGCCGGAAAATCCATTACAAGCTCCGACTACTCCCCGGTGTACGGCACCACCTACGGCCAGGCATTCTACTTCAACAACCTCTACGATCATCTAACGGCGGTGGCGAAGCGGCACCGAACGTTCCGGCTGCTCGCGTCGGCGTACAGCGAGATATACACGGTCGATCCGAGAAACGTCGAGCACGTATTGAAGACGAAATTCGACAAGTACCCGAAAGGAAGCAAAGATCAAGAACTCTGCGGGGATCTGTTTGGAGAGGGAATATTTGCGGTGGATGGAGAGAAGTGGAAGCAGCAGAGGAAATTGGCGAGCTATGAATTCTCGACGAGGGTTCTTAGGGATTTTAGCTGCTCTGTTTTCAGACGAAATGCTGCTAAACTTGTTGGACTTCTTTCCCAGCTTTCCACCTCGGGTCGGGTTTTTGATATCCAGGTTAGCACGTATTTACAAGATTTATGGAGGGAAATTGATTTTTTTTTTTTTTTTTTTTTTGAGTACATCAACATTTGAGGCGGGGGATTCGAACATCTGACGAGGATACATGTCAATTACCGTTAAGCTAAGCTCGCTTTGGCGGGAGGGAAATTGATTAAAAATAATAATTTATTTATCATTTTTTAATAGAAAAGTTAACAATAACCCAATATTAGGAACCAATTTTAAGTTTGATTTAAAGTAGAGGGATTAAAATGTTGATATATATGTTAGTACAAGAGTTAGGGTACAAAATATTGTCAACATGAGCATAGCTCAACTAGCATAACTTTTGTACTAATGATCACTCACATATTGTCAAAAAAATGGTACAAAATGCTCGTAAACTTTGAATTTTTATTTTTACCTTTTAACTTTAATTTGTTCCAATCGTTAACATAAACATAACAAAATTGGCATAAAGTTTGTACTAAAATTACTAATGACCAAAATTTATGTGGTTCTAATTTCCCACCCTCACATATTGTCCAAAAAAATGTTCAAAAAACATACAATTGCATGGGAATGGAGACGACTACTAATGTGTATAAACATTGGAAGACATATGCATCATTCATTGATAGGTAAAAATAGGTTATTTTGATGATTTTTTTAACCATATATGTTGAATAAATTAATGACCTATTAGACATAATCTAAAGTTAAATAATTTTTTTTTTTTTGTATAATATGTAAGGGATGAGATGATTCGAACCATAAACCTCCTAGTCATCAAAGTTTATGTCAGTCGAGCTATATTCATGTTGACACAACATTTAGATTTTTTTTTTTTTTTTTAAACTAAGAAAAAGGATTGTCTTCCGTTTCTTTTGTAAGGATTTGCTAATGCGATGCTCTTTGGACTCCATTTTCAAAGTCGGGTTCGGCGTGGATTTAGATTGCTTGGAGGAACCAAGCAAAGCTGGGAGCGGCTTCATGAAGGCCTTCGATGATTCTAGCGCTCAGGTTTTTTGGCGCTATATCGATCCTTTTTGGAAATTGAAGAGATTTCTCAACATCGGTTCCGAAGCTTCGTTTAGGAACAACGTAAAAGTAATCGATGCTTTTGTGCACCAGTTGATCAGTGACAGGAGAAAATTGCTTCAGCAACCAAATCTCGTAAGCTTCGTTCTCTACTTTCAGTGCCTATAAGCATTATAATCTGTAACCTTGTAGTGATGTTTGCTTTTGATGGCAGTATATTGTGATAGCACTGTTCTTTATCTCAACTTGATTTTGCTGTTCTTACTGGAGTAAAAGAGATCATTTCGTTCATAAAATGATCGTTATTGTTCCTATTATGGGAGCTACTTTACTAACGTCTAGGTATATCTAAGTTTTGAAATGTGTGCTGTAGGACGAACCAATTATACTATAAATTAGTCGAGCAATATTGGCCCAATTCCTAAACACTCATAGTTTTTTTTTTTTTCAACTTGTGTGGTGGGAGATTCGAACTACAGACCTTTTGGTCATTAATACAAGTTTTATGTCAGTTGAGTTATGCTCATGCTGGCAAACACTCATAGTTACTTGTAGTACATTAAGTTTTTTTATGACAAATATTGCAGTCTTGCGTATGTATTTTTTAATCAAAATGTCTATAAGTTAATGATGGTTAATAAAAAAGAACATCCTTCGATGTATAAGTTAAACTAGTAAATTTGAACATAGTTCAAGTGGTTAAGGTATATATCCTCGACCAAAAGGTCATATATTCAAATCCTCAGAAAAAATAACGAGTTAAACTATGTAGACTAATGTTTTCTATGGAAAAGAAGGAATTGAAACAACCATTCAGTTCAAAAGCTAAGTTGTGTTAACTAGTAGTCTTACTATCAGAATTAGAGCTTGTAGCCTTATTTGAACTCTTTACTTTTGTTAACAAGTTCGTTGAATTTTGTTTTGAACTTGAAGAAGAATGACAAAGAGGACATACTTTCGAGGTTTCTAATGGAAAGTGAGAAGGATCCAACAAGAATGAATGATCGATATCTGAGAGATATAGTTCTTAATTTCATGTTGGCTGGCAAGGATACAAGTGCAGGAACTCTGTCTTGGTTCTTCTACATGCTATGCAAGAACCCCTTAATACAGGAAAAAGTTGCAGAAGAAGTGAGACAAATTGTTGGGGTTCAAGGGGAAGAAACTGATATAAATTTGTTCGTACAGAACTTAACTGATTCAGCTCTCGACAAAATGCATTATCTTCATGCAGCACTGGCAGAGACTCTAAGGCTGTATCCTGCTGTCCCTGTGGTAAGTTTTCAAAAACCTGACATTTTGGCTGAATATGAGATAGGATGAAATTATTTAACTGTCAATTAAATGTTGTGTATAGCATGTTTAGGAACCGTTAGATGTATGTTTGAAATTTGTATGCCCCTAATAACCAACGGGTGAATGCACAAATTTGAAGTACGTTTTGTAACTTCTCTCTCATAAATATACGTTGTTACGGTCTTTTCTAACAATTTCTCTAGTTCGGGGGACTAATCGATGGATTTCTCAATGAATACTTGCAAAGCATGCGTAGTTTGTGAAATCTGCAAATCATGTCCTATAGTTGATTAGTATCTTTAGTGTTTTATAGAATTCACTTGTTATTGATATAATATCAAAGAAGTACACTAAATAATAAATAGTTTAGAGGGAATGAATTCAAACCATAGTGGCCACCTATCTAGGATTTAATATCCTTCAAGTTACCTTGACAACTAAATGTAGAAGGGTCAAGCGGTTGCCTTGTGATAATAGTCAAAGTACACGCAAGCTGACCTGAACACTCACGAAAAGAAAAGTACAAGTATGTACGTATCTAGGAAGAGATTTGAGAGTCCCTCGCTAAATCTTCTGTTCACCCTCATCCTATGATACATGTTAATTTTGAGGGCTGCTAATTTCTTGCTAAAATTGGTCATATGACACATGTAGAATTGCAACGTGTTATAGTACATTTTAATAAAATTGTCCCTTAATATTATGAAGATTTAAATCTTTCATCTAGAAATATTAGCATGTGGTTTCAATATTTCACGAGCTACTTTGTGTCATTTGATATTCTTTAATTCATTGTTGTCATAGTAACTATGGTAACAAGAATTAGTGTTAATGTGTCAAGGATGGAAGGACTGCAGAAATAGATGACATTCTTCCTAATGGCCATAAACTAAGAAAAGGGGACAGTGTGTACTACTTGGCCTATTCCATGGGCAGGATGCCCTGCCTTTGGGGAGAAGATGCTGAAGATTTTAAACCTGAGAGATGGCTTGAAAATGGAACTTTTCGACCCGAATCGCCTTTCAAATTCATCGCATTTCATGTGAGTATTGATTTTATATTGATCGTATCATCATTATTTTTTTACATGTTTTTACTGAGTTTCTTCATCTTAGGCGGGTCCTCGAATGTGTTTGGGAAAGGATTTTGCTTATCGACAAATGAAGATAGTATCTGCTGCTCTGCTTCAATTTTTTCGATTCAAATTAGCTGATCCGACTAGAAATATAACTTATAGGATCATGCTCACCCTCCACATCGATGGAGGTCTCCCACTTGTTGCAATTCCAAGAATAAGAAAATTGCACTATGTATTGTAACTAAGTGTATAAAGTAAGAAATACTAGTTTGTAATGTGAATGTACTCAGGTGTGTAATATGTATATATTGAAACTGACTTTTTTTTTTTTTGAAAATATATTGAAACTGACTTCATATATGTATAATAATATGTGATTTTTCATATATTGTTAAAGCTGTTACATAAGTTAAACTCT

mRNA sequence

ATGATCGTACTCTCGACTTCTGAATCCATCTTCTTCAGCCAAAAAATCCCCAAAAATTTCTCAAAGAATATCTCCCGCTTCGTGATTCTCTCATCCAATCATTGCGATTCAGCCATGGAAGTGAATTTCAACATCTTCACCGTCGTTTCCATCGCACTACTCACAGTCTCCCTCTTCCTTCTGTCCTTGTTCATTCTCCTCCTCCGAACCCTCGCCGGAAAATCCATTACAAGCTCCGACTACTCCCCGGTGTACGGCACCACCTACGGCCAGGCATTCTACTTCAACAACCTCTACGATCATCTAACGGCGGTGGCGAAGCGGCACCGAACGTTCCGGCTGCTCGCGTCGGCGTACAGCGAGATATACACGGTCGATCCGAGAAACGTCGAGCACGTATTGAAGACGAAATTCGACAAGTACCCGAAAGGAAGCAAAGATCAAGAACTCTGCGGGGATCTGTTTGGAGAGGGAATATTTGCGGTGGATGGAGAGAAGTGGAAGCAGCAGAGGAAATTGGCGAGCTATGAATTCTCGACGAGGGTTCTTAGGGATTTTAGCTGCTCTGTTTTCAGACGAAATGCTGCTAAACTTGTTGGACTTCTTTCCCAGCTTTCCACCTCGGGTCGGGTTTTTGATATCCAGGATTTGCTAATGCGATGCTCTTTGGACTCCATTTTCAAAGTCGGGTTCGGCGTGGATTTAGATTGCTTGGAGGAACCAAGCAAAGCTGGGAGCGGCTTCATGAAGGCCTTCGATGATTCTAGCGCTCAGGTTTTTTGGCGCTATATCGATCCTTTTTGGAAATTGAAGAGATTTCTCAACATCGGTTCCGAAGCTTCGTTTAGGAACAACGTAAAAGTAATCGATGCTTTTGTGCACCAGTTGATCAGTGACAGGAGAAAATTGCTTCAGCAACCAAATCTCCACTGTTCTTTATCTCAACTTGATTTTGCTGTTCTTACTGGAGTAAAAGAGATCATTTCTTCGTTGAATTTTGTTTTGAACTTGAAGAAGAATGACAAAGAGGACATACTTTCGAGGTTTCTAATGGAAAGTGAGAAGGATCCAACAAGAATGAATGATCGATATCTGAGAGATATAGTTCTTAATTTCATGTTGGCTGGCAAGGATACAAGTGCAGGAACTCTGTCTTGGTTCTTCTACATGCTATGCAAGAACCCCTTAATACAGGAAAAAGTTGCAGAAGAAGTGAGACAAATTGTTGGGGTTCAAGGGGAAGAAACTGATATAAATTTGTTCGTACAGAACTTAACTGATTCAGCTCTCGACAAAATGCATTATCTTCATGCAGCACTGGCAGAGACTCTAAGGCTGTATCCTGCTGTCCCTGTGGATGGAAGGACTGCAGAAATAGATGACATTCTTCCTAATGGCCATAAACTAAGAAAAGGGGACAGTGTGTACTACTTGGCCTATTCCATGGGCAGGATGCCCTGCCTTTGGGGAGAAGATGCTGAAGATTTTAAACCTGAGAGATGGCTTGAAAATGGAACTTTTCGACCCGAATCGCCTTTCAAATTCATCGCATTTCATGCGGGTCCTCGAATGTGTTTGGGAAAGGATTTTGCTTATCGACAAATGAAGATAGTATCTGCTGCTCTGCTTCAATTTTTTCGATTCAAATTAGCTGATCCGACTAGAAATATAACTTATAGGATCATGCTCACCCTCCACATCGATGGAGGTCTCCCACTTGTTGCAATTCCAAGAATAAGAAAATTGCACTATGTATTGTAA

Coding sequence (CDS)

ATGATCGTACTCTCGACTTCTGAATCCATCTTCTTCAGCCAAAAAATCCCCAAAAATTTCTCAAAGAATATCTCCCGCTTCGTGATTCTCTCATCCAATCATTGCGATTCAGCCATGGAAGTGAATTTCAACATCTTCACCGTCGTTTCCATCGCACTACTCACAGTCTCCCTCTTCCTTCTGTCCTTGTTCATTCTCCTCCTCCGAACCCTCGCCGGAAAATCCATTACAAGCTCCGACTACTCCCCGGTGTACGGCACCACCTACGGCCAGGCATTCTACTTCAACAACCTCTACGATCATCTAACGGCGGTGGCGAAGCGGCACCGAACGTTCCGGCTGCTCGCGTCGGCGTACAGCGAGATATACACGGTCGATCCGAGAAACGTCGAGCACGTATTGAAGACGAAATTCGACAAGTACCCGAAAGGAAGCAAAGATCAAGAACTCTGCGGGGATCTGTTTGGAGAGGGAATATTTGCGGTGGATGGAGAGAAGTGGAAGCAGCAGAGGAAATTGGCGAGCTATGAATTCTCGACGAGGGTTCTTAGGGATTTTAGCTGCTCTGTTTTCAGACGAAATGCTGCTAAACTTGTTGGACTTCTTTCCCAGCTTTCCACCTCGGGTCGGGTTTTTGATATCCAGGATTTGCTAATGCGATGCTCTTTGGACTCCATTTTCAAAGTCGGGTTCGGCGTGGATTTAGATTGCTTGGAGGAACCAAGCAAAGCTGGGAGCGGCTTCATGAAGGCCTTCGATGATTCTAGCGCTCAGGTTTTTTGGCGCTATATCGATCCTTTTTGGAAATTGAAGAGATTTCTCAACATCGGTTCCGAAGCTTCGTTTAGGAACAACGTAAAAGTAATCGATGCTTTTGTGCACCAGTTGATCAGTGACAGGAGAAAATTGCTTCAGCAACCAAATCTCCACTGTTCTTTATCTCAACTTGATTTTGCTGTTCTTACTGGAGTAAAAGAGATCATTTCTTCGTTGAATTTTGTTTTGAACTTGAAGAAGAATGACAAAGAGGACATACTTTCGAGGTTTCTAATGGAAAGTGAGAAGGATCCAACAAGAATGAATGATCGATATCTGAGAGATATAGTTCTTAATTTCATGTTGGCTGGCAAGGATACAAGTGCAGGAACTCTGTCTTGGTTCTTCTACATGCTATGCAAGAACCCCTTAATACAGGAAAAAGTTGCAGAAGAAGTGAGACAAATTGTTGGGGTTCAAGGGGAAGAAACTGATATAAATTTGTTCGTACAGAACTTAACTGATTCAGCTCTCGACAAAATGCATTATCTTCATGCAGCACTGGCAGAGACTCTAAGGCTGTATCCTGCTGTCCCTGTGGATGGAAGGACTGCAGAAATAGATGACATTCTTCCTAATGGCCATAAACTAAGAAAAGGGGACAGTGTGTACTACTTGGCCTATTCCATGGGCAGGATGCCCTGCCTTTGGGGAGAAGATGCTGAAGATTTTAAACCTGAGAGATGGCTTGAAAATGGAACTTTTCGACCCGAATCGCCTTTCAAATTCATCGCATTTCATGCGGGTCCTCGAATGTGTTTGGGAAAGGATTTTGCTTATCGACAAATGAAGATAGTATCTGCTGCTCTGCTTCAATTTTTTCGATTCAAATTAGCTGATCCGACTAGAAATATAACTTATAGGATCATGCTCACCCTCCACATCGATGGAGGTCTCCCACTTGTTGCAATTCCAAGAATAAGAAAATTGCACTATGTATTGTAA

Protein sequence

MIVLSTSESIFFSQKIPKNFSKNISRFVILSSNHCDSAMEVNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNLYDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEGIFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLLMRCSLDSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQEKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAEIDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAIPRIRKLHYVL
Homology
BLAST of Spg031830 vs. NCBI nr
Match: XP_038877606.1 (cytochrome P450 704C1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 915.2 bits (2364), Expect = 2.8e-262
Identity = 449/542 (82.84%), Postives = 483/542 (89.11%), Query Frame = 0

Query: 39  MEVNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNL 98
           MEV  N FT+ SI     SLF L  FILLL+T AGKSIT+SDYSPVYGT YGQAFYFNNL
Sbjct: 1   MEVIINTFTLFSIVF---SLFFLFFFILLLQTFAGKSITNSDYSPVYGTIYGQAFYFNNL 60

Query: 99  YDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEG 158
           YDHLTAVAKRH+TFRL+ +AYSEIYTVDPRN+E +LKTKFDKY KG KDQE+CGDLFGEG
Sbjct: 61  YDHLTAVAKRHKTFRLVGTAYSEIYTVDPRNIEQILKTKFDKYRKGRKDQEVCGDLFGEG 120

Query: 159 IFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLL 218
           IFAVDGEKWKQQRKLASYEFST++LRDFSCSVFRRN AKLVG++S+ + + RVFD+QDLL
Sbjct: 121 IFAVDGEKWKQQRKLASYEFSTKILRDFSCSVFRRNGAKLVGIVSEFAATARVFDVQDLL 180

Query: 219 MRCSLDSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGS 278
           MRCSLDSIFKVGFGVDL+C+EEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGS
Sbjct: 181 MRCSLDSIFKVGFGVDLNCMEEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGS 240

Query: 279 EASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLK 338
           EASFRNN+K+IDAF+H LIS RRKLL QPNL                             
Sbjct: 241 EASFRNNLKLIDAFMHHLISARRKLLHQPNL----------------------------- 300

Query: 339 KNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQ 398
           KNDKEDILSRFLMESEKDPTRMND+YLRDIVLNFMLAG+DTSAGTLSWFFYMLCKNPLIQ
Sbjct: 301 KNDKEDILSRFLMESEKDPTRMNDQYLRDIVLNFMLAGRDTSAGTLSWFFYMLCKNPLIQ 360

Query: 399 EKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAE 458
           EKVAEEV QIVGVQGEE DINLF+QNLTDSA+DKMHYLHAAL ETLRLYPAVPVDGRTAE
Sbjct: 361 EKVAEEVSQIVGVQGEEADINLFIQNLTDSAIDKMHYLHAALTETLRLYPAVPVDGRTAE 420

Query: 459 IDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAF 518
           IDD+LP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENG+FRPESPFKFIAF
Sbjct: 421 IDDVLPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGSFRPESPFKFIAF 480

Query: 519 HAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAIPR 578
           HAGPR CLGKDFAYRQMKIVSAALLQFFRFKLADPTRN+TYRIMLTLHIDGGLPL+A+PR
Sbjct: 481 HAGPRTCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNVTYRIMLTLHIDGGLPLLAVPR 510

Query: 579 IR 581
           +R
Sbjct: 541 VR 510

BLAST of Spg031830 vs. NCBI nr
Match: XP_008438136.1 (PREDICTED: cytochrome P450 704C1-like isoform X1 [Cucumis melo])

HSP 1 Score: 913.7 bits (2360), Expect = 8.2e-262
Identity = 450/545 (82.57%), Postives = 483/545 (88.62%), Query Frame = 0

Query: 39  MEVNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNL 98
           MEVNFNI T  ++ L    LF LS FIL L+TLAGKSIT+SDYSPVYGT YGQAFY NNL
Sbjct: 1   MEVNFNIITFFTVVL---CLFFLSFFILFLKTLAGKSITNSDYSPVYGTIYGQAFYLNNL 60

Query: 99  YDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEG 158
           YDHLTAVAKRHRTFRLL  +YSEIYTVDPRNVEH+LKTKF+ Y KG KDQE+CGDLFGEG
Sbjct: 61  YDHLTAVAKRHRTFRLLGESYSEIYTVDPRNVEHILKTKFENYMKGRKDQEVCGDLFGEG 120

Query: 159 IFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLL 218
           IFAVDGEKWK+QRKLASYEFST++LRDFSCSVFRRNA KLVG++S+ ST  R+FD+QDLL
Sbjct: 121 IFAVDGEKWKEQRKLASYEFSTKILRDFSCSVFRRNAEKLVGIVSEFSTMARIFDVQDLL 180

Query: 219 MRCSLDSIFKVGFGVDLDCLEEPSKAG--SGFMKAFDDSSAQVFWRYIDPFWKLKRFLNI 278
           MRCSLDSIFKVGFGVDL+C+EEPSKA    GFM+AFDD+SAQVFWRYIDPFWKLKRFLNI
Sbjct: 181 MRCSLDSIFKVGFGVDLNCMEEPSKAAGRGGFMEAFDDASAQVFWRYIDPFWKLKRFLNI 240

Query: 279 GSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLN 338
           GSEASFRNN+K+IDAFVHQLIS RRKLL QPNL                           
Sbjct: 241 GSEASFRNNLKIIDAFVHQLISARRKLLHQPNL--------------------------- 300

Query: 339 LKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPL 398
             K DKEDILSRFLMESEKDPTRMND+YLRDI+LNFMLAG+DTSAGTLSWFFYMLCKNPL
Sbjct: 301 --KIDKEDILSRFLMESEKDPTRMNDQYLRDIILNFMLAGRDTSAGTLSWFFYMLCKNPL 360

Query: 399 IQEKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRT 458
           IQEKVAEEV QIVGVQGEETDINLFVQNLTDSALDKMHYLHAAL ETLRLYPAVP+DGRT
Sbjct: 361 IQEKVAEEVSQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALTETLRLYPAVPIDGRT 420

Query: 459 AEIDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 518
           AE DDILP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI
Sbjct: 421 AETDDILPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 480

Query: 519 AFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAI 578
           +FHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRN+TYRIMLTLHIDGGLPL+A+
Sbjct: 481 SFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNVTYRIMLTLHIDGGLPLLAL 513

Query: 579 PRIRK 582
           PR+RK
Sbjct: 541 PRVRK 513

BLAST of Spg031830 vs. NCBI nr
Match: XP_038877612.1 (cytochrome P450 704C1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 912.9 bits (2358), Expect = 1.4e-261
Identity = 448/542 (82.66%), Postives = 482/542 (88.93%), Query Frame = 0

Query: 39  MEVNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNL 98
           MEV  N FT+ SI     SLF L  FILLL+T AGKSIT+SDYSPVYGT YGQAFYFNNL
Sbjct: 1   MEVIINTFTLFSIVF---SLFFLFFFILLLQTFAGKSITNSDYSPVYGTIYGQAFYFNNL 60

Query: 99  YDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEG 158
           YDHLTAVAKRH+TFRL+ +AYSEIYTVDPRN+E +LKTKFDKY KG KDQE+CGDLFGEG
Sbjct: 61  YDHLTAVAKRHKTFRLVGTAYSEIYTVDPRNIEQILKTKFDKYRKGRKDQEVCGDLFGEG 120

Query: 159 IFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLL 218
           IFAVDGEKWKQQRKLASYEFST++LRDFSCSVFRRN AKLVG++S+ + + RVFD+QDLL
Sbjct: 121 IFAVDGEKWKQQRKLASYEFSTKILRDFSCSVFRRNGAKLVGIVSEFAATARVFDVQDLL 180

Query: 219 MRCSLDSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGS 278
           MRCSLDSIFKVGFGVDL+C+EEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGS
Sbjct: 181 MRCSLDSIFKVGFGVDLNCMEEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGS 240

Query: 279 EASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLK 338
           EASFRNN+K+IDAF+H LIS RRKLL QPNL                             
Sbjct: 241 EASFRNNLKLIDAFMHHLISARRKLLHQPNL----------------------------- 300

Query: 339 KNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQ 398
            NDKEDILSRFLMESEKDPTRMND+YLRDIVLNFMLAG+DTSAGTLSWFFYMLCKNPLIQ
Sbjct: 301 -NDKEDILSRFLMESEKDPTRMNDQYLRDIVLNFMLAGRDTSAGTLSWFFYMLCKNPLIQ 360

Query: 399 EKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAE 458
           EKVAEEV QIVGVQGEE DINLF+QNLTDSA+DKMHYLHAAL ETLRLYPAVPVDGRTAE
Sbjct: 361 EKVAEEVSQIVGVQGEEADINLFIQNLTDSAIDKMHYLHAALTETLRLYPAVPVDGRTAE 420

Query: 459 IDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAF 518
           IDD+LP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENG+FRPESPFKFIAF
Sbjct: 421 IDDVLPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGSFRPESPFKFIAF 480

Query: 519 HAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAIPR 578
           HAGPR CLGKDFAYRQMKIVSAALLQFFRFKLADPTRN+TYRIMLTLHIDGGLPL+A+PR
Sbjct: 481 HAGPRTCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNVTYRIMLTLHIDGGLPLLAVPR 509

Query: 579 IR 581
           +R
Sbjct: 541 VR 509

BLAST of Spg031830 vs. NCBI nr
Match: XP_008438137.1 (PREDICTED: cytochrome P450 704C1-like isoform X2 [Cucumis melo])

HSP 1 Score: 911.4 bits (2354), Expect = 4.1e-261
Identity = 449/545 (82.39%), Postives = 482/545 (88.44%), Query Frame = 0

Query: 39  MEVNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNL 98
           MEVNFNI T  ++ L    LF LS FIL L+TLAGKSIT+SDYSPVYGT YGQAFY NNL
Sbjct: 1   MEVNFNIITFFTVVL---CLFFLSFFILFLKTLAGKSITNSDYSPVYGTIYGQAFYLNNL 60

Query: 99  YDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEG 158
           YDHLTAVAKRHRTFRLL  +YSEIYTVDPRNVEH+LKTKF+ Y KG KDQE+CGDLFGEG
Sbjct: 61  YDHLTAVAKRHRTFRLLGESYSEIYTVDPRNVEHILKTKFENYMKGRKDQEVCGDLFGEG 120

Query: 159 IFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLL 218
           IFAVDGEKWK+QRKLASYEFST++LRDFSCSVFRRNA KLVG++S+ ST  R+FD+QDLL
Sbjct: 121 IFAVDGEKWKEQRKLASYEFSTKILRDFSCSVFRRNAEKLVGIVSEFSTMARIFDVQDLL 180

Query: 219 MRCSLDSIFKVGFGVDLDCLEEPSKAG--SGFMKAFDDSSAQVFWRYIDPFWKLKRFLNI 278
           MRCSLDSIFKVGFGVDL+C+EEPSKA    GFM+AFDD+SAQVFWRYIDPFWKLKRFLNI
Sbjct: 181 MRCSLDSIFKVGFGVDLNCMEEPSKAAGRGGFMEAFDDASAQVFWRYIDPFWKLKRFLNI 240

Query: 279 GSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLN 338
           GSEASFRNN+K+IDAFVHQLIS RRKLL QPNL                           
Sbjct: 241 GSEASFRNNLKIIDAFVHQLISARRKLLHQPNL--------------------------- 300

Query: 339 LKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPL 398
               DKEDILSRFLMESEKDPTRMND+YLRDI+LNFMLAG+DTSAGTLSWFFYMLCKNPL
Sbjct: 301 ---IDKEDILSRFLMESEKDPTRMNDQYLRDIILNFMLAGRDTSAGTLSWFFYMLCKNPL 360

Query: 399 IQEKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRT 458
           IQEKVAEEV QIVGVQGEETDINLFVQNLTDSALDKMHYLHAAL ETLRLYPAVP+DGRT
Sbjct: 361 IQEKVAEEVSQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALTETLRLYPAVPIDGRT 420

Query: 459 AEIDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 518
           AE DDILP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI
Sbjct: 421 AETDDILPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 480

Query: 519 AFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAI 578
           +FHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRN+TYRIMLTLHIDGGLPL+A+
Sbjct: 481 SFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNVTYRIMLTLHIDGGLPLLAL 512

Query: 579 PRIRK 582
           PR+RK
Sbjct: 541 PRVRK 512

BLAST of Spg031830 vs. NCBI nr
Match: XP_004133900.1 (cytochrome P450 704C1 isoform X1 [Cucumis sativus] >KGN56579.1 hypothetical protein Csa_009529 [Cucumis sativus])

HSP 1 Score: 902.9 bits (2332), Expect = 1.5e-258
Identity = 446/543 (82.14%), Postives = 484/543 (89.13%), Query Frame = 0

Query: 41  VNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNLYD 100
           +NFNI T  SI L    L  LS FILLL+TL+GKSIT+SDYSPVYGT YGQAFYFNNLYD
Sbjct: 4   INFNIITFFSILL---CLLFLSFFILLLKTLSGKSITNSDYSPVYGTIYGQAFYFNNLYD 63

Query: 101 HLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEGIF 160
           HLTAVAKRHRTFRL+ ++YSEIYTV+PRN+EH+LKTKF+KY KGSKDQE+ GDLFGEGIF
Sbjct: 64  HLTAVAKRHRTFRLIGASYSEIYTVNPRNIEHILKTKFEKYMKGSKDQEVGGDLFGEGIF 123

Query: 161 AVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLLMR 220
           AVDGEKWK+QRKLASYE ST++LRDFSCSVFRRNA KLVG++S+ ST  RVFD+QDLLMR
Sbjct: 124 AVDGEKWKEQRKLASYELSTKILRDFSCSVFRRNAEKLVGIISEFSTMARVFDVQDLLMR 183

Query: 221 CSLDSIFKVGFGVDLDCLEEPSKAGS--GFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGS 280
           CSLDSIFKVGFGVDL+C+EEPSKA    GFM+AFD++SAQVFWR+IDPFWKLKRFLN+GS
Sbjct: 184 CSLDSIFKVGFGVDLNCVEEPSKAAGRRGFMEAFDNASAQVFWRFIDPFWKLKRFLNVGS 243

Query: 281 EASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLK 340
           EASFRNN+K+IDAFVHQLIS RRKLL QPNL                             
Sbjct: 244 EASFRNNLKIIDAFVHQLISARRKLLHQPNL----------------------------- 303

Query: 341 KNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQ 400
           K DKEDILSRFLMESEKDPTRMND+YLRDIVLNFMLAG+DTSAGTLSWFFYMLCKNPLIQ
Sbjct: 304 KIDKEDILSRFLMESEKDPTRMNDQYLRDIVLNFMLAGRDTSAGTLSWFFYMLCKNPLIQ 363

Query: 401 EKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAE 460
           EKVAEEV QIVGVQGEETDI+LF+QNLTDSALDKMHYLHAAL ETLRLYPAVPVDGRTAE
Sbjct: 364 EKVAEEVSQIVGVQGEETDIHLFIQNLTDSALDKMHYLHAALTETLRLYPAVPVDGRTAE 423

Query: 461 IDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAF 520
           IDDILP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAF
Sbjct: 424 IDDILPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAF 483

Query: 521 HAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAIPR 580
           HAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRN+TYRIMLTLHIDGGLPL+A+PR
Sbjct: 484 HAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNVTYRIMLTLHIDGGLPLLALPR 514

Query: 581 IRK 582
           +RK
Sbjct: 544 VRK 514

BLAST of Spg031830 vs. ExPASy Swiss-Prot
Match: Q50EK3 (Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 3.0e-134
Identity = 265/554 (47.83%), Postives = 368/554 (66.43%), Query Frame = 0

Query: 39  MEVN-FNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNN 98
           M+VN   +F  VS   L  SL++ S     LR    K +    Y PV GT    A  F  
Sbjct: 1   MDVNILTMFVTVSALALACSLWIAS----YLRNWRKKGV----YPPVVGTMLNHAINFER 60

Query: 99  LYDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGE 158
           L+D+ T  A+R++TFR++    S ++T DP NVEH+LKT F  Y KG+ + ++  DL G+
Sbjct: 61  LHDYHTDQAQRYKTFRVVYPTCSYVFTTDPVNVEHILKTNFANYDKGTFNYDIMKDLLGD 120

Query: 159 GIFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDL 218
           GIF VDG+KW+QQRKLAS EF+++VL+DFS  VF  NAAKL  +L+Q +      ++QDL
Sbjct: 121 GIFNVDGDKWRQQRKLASSEFASKVLKDFSSGVFCNNAAKLANILAQAAKLNLSVEMQDL 180

Query: 219 LMRCSLDSIFKVGFGVDLDCLEEPSKAGSG----FMKAFDDSSAQVFWRY-IDPFWKLKR 278
            MR SLDSI KV FG+D++ L   SKA SG    F KAFD ++A VF R+ +  FWK++R
Sbjct: 181 FMRSSLDSICKVVFGIDINSLSS-SKAESGPEASFAKAFDVANAMVFHRHMVGSFWKVQR 240

Query: 279 FLNIGSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLN 338
           F N+GSEA  R+N+K++D F++++I  RR                       +E+ S+  
Sbjct: 241 FFNVGSEAILRDNIKMVDDFLYKVIHFRR-----------------------QEMFSA-- 300

Query: 339 FVLNLKKNDKEDILSRFLMESEKDPT-RMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYML 398
                K+N + DILSR+++ S+K+   +++D+YLRD++LNFM+A +DT+A  LSWF YML
Sbjct: 301 ----EKENVRPDILSRYIIISDKETDGKVSDKYLRDVILNFMVAARDTTAIALSWFIYML 360

Query: 399 CKNPLIQEKVAEEVRQIVGVQGEE-----TDINLFVQNLTDSALDKMHYLHAALAETLRL 458
           CK+  +QEK+ EE+     V  ++      DI  F Q+LTD AL KMHYLHA+L+ETLRL
Sbjct: 361 CKHQHVQEKLLEEIISSTSVHEDQYSTECNDIASFAQSLTDEALGKMHYLHASLSETLRL 420

Query: 459 YPAVPVDGRTAEIDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGT 518
           YPA+PVDG+    +D LP+G K++KGDSV +L Y+MGRM  LWG+DA++FKPERW+++G 
Sbjct: 421 YPALPVDGKYVVNEDTLPDGFKVKKGDSVNFLPYAMGRMSYLWGDDAKEFKPERWIQDGI 480

Query: 519 FRPESPFKFIAFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLH 578
           F P+SPFKF AF AGPR CLGKDFAY QMKIV+A L++FF+F+ A  T+ + YR MLTLH
Sbjct: 481 FHPKSPFKFPAFQAGPRTCLGKDFAYLQMKIVAAVLVRFFKFE-AVKTKEVRYRTMLTLH 515

Query: 579 I-DGGLPLVAIPRI 580
           + + GL +   PR+
Sbjct: 541 MNEDGLNVQVTPRL 515

BLAST of Spg031830 vs. ExPASy Swiss-Prot
Match: Q9C788 (Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 6.0e-106
Identity = 219/513 (42.69%), Postives = 296/513 (57.70%), Query Frame = 0

Query: 83  PVYGTTYGQAFYFNNLYDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYP 142
           P+ G    Q   F+ ++D L       RT  +     +  Y  DP NVE+VLKT F  YP
Sbjct: 32  PLVGAAIEQLTNFDRMHDWLVEYLYNSRTVVVPMPFTTYTYIADPINVEYVLKTNFSNYP 91

Query: 143 KGSKDQELCGDLFGEGIFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLL 202
           KG         L G+GIF  DGE W++QRK AS+EF+++ LRDFS  VF+  + KL  +L
Sbjct: 92  KGETYHSYMEVLLGDGIFNSDGELWRKQRKTASFEFASKNLRDFSTVVFKEYSLKLFTIL 151

Query: 203 SQLSTSGRVFDIQDLLMRCSLDSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWR 262
           SQ S   +  D+Q+LLMR +LDSI KVGFGV++  L  P    + F KAFD ++  V  R
Sbjct: 152 SQASFKEQQVDMQELLMRMTLDSICKVGFGVEIGTL-APELPENHFAKAFDTANIIVTLR 211

Query: 263 YIDPFWKLKRFLNIGSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLT 322
           +IDP WK+K+FLNIGSEA    ++KV++ F + +I  R+  L +                
Sbjct: 212 FIDPLWKMKKFLNIGSEALLGKSIKVVNDFTYSVIRRRKAELLEAQ-------------- 271

Query: 323 GVKEIISSLNFVLNLKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAG 382
                IS  N   N     K DILSRF+  S+   ++  ++ LRDIVLNF++AG+DT+A 
Sbjct: 272 -----ISPTNNNNNNNNKVKHDILSRFIEISDDPDSKETEKSLRDIVLNFVIAGRDTTAT 331

Query: 383 TLSWFFYMLCKNPLIQEKVAEEVRQIVGVQGEET----------DINLFVQNLTDSA--- 442
           TL+W  YM+  N  + EK+  E++++     E T          D N F + +T+ A   
Sbjct: 332 TLTWAIYMIMMNENVAEKLYSELQELEKESAEATNTSLHQYDTEDFNSFNEKVTEFAGLL 391

Query: 443 ----LDKMHYLHAALAETLRLYPAVPVDGRTAEIDDILPNGHKLRKGDSVYYLAYSMGRM 502
               L K+HYLHA + ETLRLYPAVP D +    DD+LPNG K++ G  V Y+ YSMGRM
Sbjct: 392 NYDSLGKLHYLHAVITETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMVTYVPYSMGRM 451

Query: 503 PCLWGEDAEDFKPERWLENGTFRPESPFKFIAFHAGPRMCLGKDFAYRQMKIVSAALLQF 562
              WG DA  FKPERWL++G F+  SPFKF AF AGPR+CLGKD AY QMK+  A L +F
Sbjct: 452 EYNWGSDAALFKPERWLKDGVFQNASPFKFTAFQAGPRICLGKDSAYLQMKMAMAILCRF 511

Query: 563 FRFKLADPTRNITYRIMLTLHIDGGLPLVAIPR 579
           ++F L  P   + YR+M  L +  GL +    R
Sbjct: 512 YKFHLV-PNHPVKYRMMTILSMAHGLKVTVSRR 523

BLAST of Spg031830 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.8e-86
Identity = 196/571 (34.33%), Postives = 315/571 (55.17%), Query Frame = 0

Query: 19  NFSKNISRFVILSSNHCDSAMEVNFNIFTVVSIALLTVSLFLLSLFILL-LRTLAGKSIT 78
           N S N++   +  S+   S   V+  +F +  + +L +   L+++F+ + +  L  K   
Sbjct: 4   NSSYNLTFNDVFFSSSSSSDPLVSRRLFLLRDVQILEL---LIAIFVFVAIHALRQKKYQ 63

Query: 79  SSDYSPVYGTTYGQAFYF-NNLYDHLTAV-AKRHRTFRLLASAYSEI---YTVDPRNVEH 138
                P  G     AF    N+Y+ L+ V   ++ TF+     +S +    T DPRNVEH
Sbjct: 64  GLPVWPFLGMLPSLAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEH 123

Query: 139 VLKTKFDKYPKGSKDQELCGDLFGEGIFAVDGEKWKQQRKLASYEFSTRVLRDFSC-SVF 198
           +LK +F  +PKGS  ++   DL G+GIF  D E W++QRK AS EF +   R  +  S+F
Sbjct: 124 LLKNRFSVFPKGSYFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLF 183

Query: 199 RRNAAKLVGLLSQLSTSGRVFDIQDLLMRCSLDSIFKVGFGVDLDCLEEPSKAGSGFMKA 258
                +L+ +L     S    D+QD+L+R + D++  + FGVD  CL  P +    F KA
Sbjct: 184 ELVHKRLLPVLETSVKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCL-GPDQPVIPFAKA 243

Query: 259 FDDSSAQVFWRYIDP--FWKLKRFLNIGSEASFRNNVKVIDAFVHQLISDRRKLLQQPNL 318
           F+D++     R++ P   WK  R+L+IG+E   + ++K +D F  ++I  R+K L     
Sbjct: 244 FEDATEAAVVRFVMPTCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELS---- 303

Query: 319 HCSLSQLDFAVLTGVKEIISSLNFVLNLKKNDKEDILSRFLMESEKDPTRMNDRYLRDIV 378
                                    L  +   + D+L+ F+   ++     +D++LRDI 
Sbjct: 304 -------------------------LEGETTKRSDLLTVFMGLRDEKGESFSDKFLRDIC 363

Query: 379 LNFMLAGKDTSAGTLSWFFYMLCKNPLIQEKVAEEVRQIVGVQGE--ETDINLFVQNLTD 438
           +NF+LAG+DTS+  LSWFF++L KNP ++EK+  E+ +I+  + +    + + +      
Sbjct: 364 VNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKILRQRDDHGNAEKSDYEPVFGP 423

Query: 439 SALDKMHYLHAALAETLRLYPAVPVDGRTAEIDDILPNGHKLRKGDSVYYLAYSMGRMPC 498
             + KM YL AAL+E LRLYP+VPVD +  + DD+ P+G  L+KGD V Y  Y+MGRM  
Sbjct: 424 EEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGTMLKKGDKVIYAIYAMGRMEA 483

Query: 499 LWGEDAEDFKPERWLENGTFRPESPFKFIAFHAGPRMCLGKDFAYRQMKIVSAALLQFFR 558
           +WG+D  +F+PERWL +G F  ES +KF AF+ GPR+CLGKDFAY QMK  +AA++  ++
Sbjct: 484 IWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLCLGKDFAYYQMKSTAAAIVYRYK 540

Query: 559 FKLADPTRNITYRIMLTLHIDGGLPLVAIPR 579
            K+ +    +  ++ LT+++  GL +  I R
Sbjct: 544 VKVVN-GHKVEPKLALTMYMKHGLMVNLINR 540

BLAST of Spg031830 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 1.6e-82
Identity = 170/455 (37.36%), Postives = 267/455 (58.68%), Query Frame = 0

Query: 124 TVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEGIFAVDGEKWKQQRKLASYEFSTRVL 183
           T DP+N+EH+LKT+FD YPKG   Q +  DL G+GIF  DG+ W+ QRK A+ EF+TR L
Sbjct: 84  TCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIFNSDGDTWRFQRKTAALEFTTRTL 143

Query: 184 RDFSCS-VFRRNAAKLVGLLSQLSTSGRVFDIQDLLMRCSLDSIFKVGFGVDLDCLEEPS 243
           R      V R    +LV +L    +     D+QD+L+R + D+I  + FG D   L  P 
Sbjct: 144 RQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLLRLTFDNICGLTFGKDPRTL-SPE 203

Query: 244 KAGSGFMKAFDDSSAQVFWRYIDP--FWKLKRFLNIGSEASFRNNVKVIDAFVHQLISDR 303
              +GF  AFD ++     R+I P   WK++++L +G E     ++  +D ++ ++I+ R
Sbjct: 204 FPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGLEDDMSRSISHVDNYLSEIINTR 263

Query: 304 R-KLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLKKNDKEDILSRFLMESEKDPTR 363
           + +LL Q                                ++  +D+LSRF+ + E     
Sbjct: 264 KLELLGQQQ-----------------------------DESRHDDLLSRFMKKKES---- 323

Query: 364 MNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQEKVAEEVRQIVGVQGEETDIN 423
            +D+YL+ + LNF+LAG+DTS+  +SWFF+++  NP ++EK+  E+  I+ ++  +T+++
Sbjct: 324 YSDKYLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTIL-IKTRDTNVS 383

Query: 424 LFV-QNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAEIDDILPNGHKLRKGDSVYYL 483
            +  + LT   +D++ YL AAL+ETLRLYP+VP D +    +D+LP+G  +  G +V Y 
Sbjct: 384 KWTDEPLTFDEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYS 443

Query: 484 AYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAFHAGPRMCLGKDFAYRQMKIV 543
            YS+GRM  +WGED  +FKPERWLE       + +KF+AF+AGPR+CLGKD AY QMK +
Sbjct: 444 IYSVGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSI 502

Query: 544 SAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPL 574
           +A++L   R  +A P   +  ++ LTL +  GL +
Sbjct: 504 TASILLRHRLTVA-PGHRVEQKMSLTLFMKFGLKM 502

BLAST of Spg031830 vs. ExPASy Swiss-Prot
Match: Q9ZUX1 (Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.3e-81
Identity = 192/537 (35.75%), Postives = 286/537 (53.26%), Query Frame = 0

Query: 46  FTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNLYDHLTAV 105
           FT+VS   + +      LF+  LR        + +    Y T+  +   F NL D  T +
Sbjct: 7   FTIVSFFFIIIFSLFHLLFLQKLR------YCNCEICHAYLTSSWKK-DFINLSDWYTHL 66

Query: 106 AKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEGIFAVDGE 165
            +R  T  +     + + T +P NVEH+LKT F  YPKG +   + GDL G GIF  DG+
Sbjct: 67  LRRSPTSTIKVHVLNSVITANPSNVEHILKTNFHNYPKGKQFSVILGDLLGRGIFNSDGD 126

Query: 166 KWKQQRKLASYEFSTRVLRDFSCSVFRRN-AAKLVGLLSQLSTS-GRVFDIQDLLMRCSL 225
            W+ QRKLAS E  +  +R F+  + +     +L+ +L+  S + G V D+QD+  R S 
Sbjct: 127 TWRFQRKLASLELGSVSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDLQDVFRRFSF 186

Query: 226 DSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWRYIDPF---WKLKRFLNIGSEA 285
           D+I K+ FG D DCL  P    S F  AFD +S     R + PF   WK KR L IGSE 
Sbjct: 187 DTISKLSFGFDPDCLRLPFPI-SEFAVAFDTASLLSAKRALAPFPLLWKTKRLLRIGSEK 246

Query: 286 SFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLKKN 345
             + ++ VI+     LI  RR                   LTG+                
Sbjct: 247 KLQESINVINRLAGDLIKQRR-------------------LTGLM--------------- 306

Query: 346 DKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQEK 405
            K D++SRF+    +D    +D YLRDIV++F+LAG+DT A  L+ FF++L ++P ++ +
Sbjct: 307 GKNDLISRFMAVVAED----DDEYLRDIVVSFLLAGRDTVAAGLTGFFWLLTRHPEVENR 366

Query: 406 VAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAEID 465
           + EE+ +++G   +               + +M YLHA+L E++RL+P V  D + A  D
Sbjct: 367 IREELDRVMGTGFDSVTARC-------DEMREMDYLHASLYESMRLFPPVQFDSKFALND 426

Query: 466 DILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLEN-GTFRPESPFKFIAFH 525
           D+L +G  +  G  V Y AY+MGRM  +WG D E+FKPERWL+N G FRPE+P K+  F 
Sbjct: 427 DVLSDGTFVNSGTRVTYHAYAMGRMDRIWGPDYEEFKPERWLDNEGKFRPENPVKYPVFQ 486

Query: 526 AGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIM--LTLHIDGGLPLV 575
           AG R+C+GK+ A  +MK ++ A+++ F  ++A P    T R    LT  ++GGLP++
Sbjct: 487 AGARVCIGKEMAIMEMKSIAVAIIRRFETRVASPETTETLRFAPGLTATVNGGLPVM 490

BLAST of Spg031830 vs. ExPASy TrEMBL
Match: A0A1S3AW91 (cytochrome P450 704C1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483335 PE=3 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 4.0e-262
Identity = 450/545 (82.57%), Postives = 483/545 (88.62%), Query Frame = 0

Query: 39  MEVNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNL 98
           MEVNFNI T  ++ L    LF LS FIL L+TLAGKSIT+SDYSPVYGT YGQAFY NNL
Sbjct: 1   MEVNFNIITFFTVVL---CLFFLSFFILFLKTLAGKSITNSDYSPVYGTIYGQAFYLNNL 60

Query: 99  YDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEG 158
           YDHLTAVAKRHRTFRLL  +YSEIYTVDPRNVEH+LKTKF+ Y KG KDQE+CGDLFGEG
Sbjct: 61  YDHLTAVAKRHRTFRLLGESYSEIYTVDPRNVEHILKTKFENYMKGRKDQEVCGDLFGEG 120

Query: 159 IFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLL 218
           IFAVDGEKWK+QRKLASYEFST++LRDFSCSVFRRNA KLVG++S+ ST  R+FD+QDLL
Sbjct: 121 IFAVDGEKWKEQRKLASYEFSTKILRDFSCSVFRRNAEKLVGIVSEFSTMARIFDVQDLL 180

Query: 219 MRCSLDSIFKVGFGVDLDCLEEPSKAG--SGFMKAFDDSSAQVFWRYIDPFWKLKRFLNI 278
           MRCSLDSIFKVGFGVDL+C+EEPSKA    GFM+AFDD+SAQVFWRYIDPFWKLKRFLNI
Sbjct: 181 MRCSLDSIFKVGFGVDLNCMEEPSKAAGRGGFMEAFDDASAQVFWRYIDPFWKLKRFLNI 240

Query: 279 GSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLN 338
           GSEASFRNN+K+IDAFVHQLIS RRKLL QPNL                           
Sbjct: 241 GSEASFRNNLKIIDAFVHQLISARRKLLHQPNL--------------------------- 300

Query: 339 LKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPL 398
             K DKEDILSRFLMESEKDPTRMND+YLRDI+LNFMLAG+DTSAGTLSWFFYMLCKNPL
Sbjct: 301 --KIDKEDILSRFLMESEKDPTRMNDQYLRDIILNFMLAGRDTSAGTLSWFFYMLCKNPL 360

Query: 399 IQEKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRT 458
           IQEKVAEEV QIVGVQGEETDINLFVQNLTDSALDKMHYLHAAL ETLRLYPAVP+DGRT
Sbjct: 361 IQEKVAEEVSQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALTETLRLYPAVPIDGRT 420

Query: 459 AEIDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 518
           AE DDILP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI
Sbjct: 421 AETDDILPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 480

Query: 519 AFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAI 578
           +FHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRN+TYRIMLTLHIDGGLPL+A+
Sbjct: 481 SFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNVTYRIMLTLHIDGGLPLLAL 513

Query: 579 PRIRK 582
           PR+RK
Sbjct: 541 PRVRK 513

BLAST of Spg031830 vs. ExPASy TrEMBL
Match: A0A1S3AVB3 (cytochrome P450 704C1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483335 PE=3 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 2.0e-261
Identity = 449/545 (82.39%), Postives = 482/545 (88.44%), Query Frame = 0

Query: 39  MEVNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNL 98
           MEVNFNI T  ++ L    LF LS FIL L+TLAGKSIT+SDYSPVYGT YGQAFY NNL
Sbjct: 1   MEVNFNIITFFTVVL---CLFFLSFFILFLKTLAGKSITNSDYSPVYGTIYGQAFYLNNL 60

Query: 99  YDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEG 158
           YDHLTAVAKRHRTFRLL  +YSEIYTVDPRNVEH+LKTKF+ Y KG KDQE+CGDLFGEG
Sbjct: 61  YDHLTAVAKRHRTFRLLGESYSEIYTVDPRNVEHILKTKFENYMKGRKDQEVCGDLFGEG 120

Query: 159 IFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLL 218
           IFAVDGEKWK+QRKLASYEFST++LRDFSCSVFRRNA KLVG++S+ ST  R+FD+QDLL
Sbjct: 121 IFAVDGEKWKEQRKLASYEFSTKILRDFSCSVFRRNAEKLVGIVSEFSTMARIFDVQDLL 180

Query: 219 MRCSLDSIFKVGFGVDLDCLEEPSKAG--SGFMKAFDDSSAQVFWRYIDPFWKLKRFLNI 278
           MRCSLDSIFKVGFGVDL+C+EEPSKA    GFM+AFDD+SAQVFWRYIDPFWKLKRFLNI
Sbjct: 181 MRCSLDSIFKVGFGVDLNCMEEPSKAAGRGGFMEAFDDASAQVFWRYIDPFWKLKRFLNI 240

Query: 279 GSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLN 338
           GSEASFRNN+K+IDAFVHQLIS RRKLL QPNL                           
Sbjct: 241 GSEASFRNNLKIIDAFVHQLISARRKLLHQPNL--------------------------- 300

Query: 339 LKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPL 398
               DKEDILSRFLMESEKDPTRMND+YLRDI+LNFMLAG+DTSAGTLSWFFYMLCKNPL
Sbjct: 301 ---IDKEDILSRFLMESEKDPTRMNDQYLRDIILNFMLAGRDTSAGTLSWFFYMLCKNPL 360

Query: 399 IQEKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRT 458
           IQEKVAEEV QIVGVQGEETDINLFVQNLTDSALDKMHYLHAAL ETLRLYPAVP+DGRT
Sbjct: 361 IQEKVAEEVSQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALTETLRLYPAVPIDGRT 420

Query: 459 AEIDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 518
           AE DDILP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI
Sbjct: 421 AETDDILPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 480

Query: 519 AFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAI 578
           +FHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRN+TYRIMLTLHIDGGLPL+A+
Sbjct: 481 SFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNVTYRIMLTLHIDGGLPLLAL 512

Query: 579 PRIRK 582
           PR+RK
Sbjct: 541 PRVRK 512

BLAST of Spg031830 vs. ExPASy TrEMBL
Match: A0A0A0L6D6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124970 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 7.0e-259
Identity = 446/543 (82.14%), Postives = 484/543 (89.13%), Query Frame = 0

Query: 41  VNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNLYD 100
           +NFNI T  SI L    L  LS FILLL+TL+GKSIT+SDYSPVYGT YGQAFYFNNLYD
Sbjct: 4   INFNIITFFSILL---CLLFLSFFILLLKTLSGKSITNSDYSPVYGTIYGQAFYFNNLYD 63

Query: 101 HLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEGIF 160
           HLTAVAKRHRTFRL+ ++YSEIYTV+PRN+EH+LKTKF+KY KGSKDQE+ GDLFGEGIF
Sbjct: 64  HLTAVAKRHRTFRLIGASYSEIYTVNPRNIEHILKTKFEKYMKGSKDQEVGGDLFGEGIF 123

Query: 161 AVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLLMR 220
           AVDGEKWK+QRKLASYE ST++LRDFSCSVFRRNA KLVG++S+ ST  RVFD+QDLLMR
Sbjct: 124 AVDGEKWKEQRKLASYELSTKILRDFSCSVFRRNAEKLVGIISEFSTMARVFDVQDLLMR 183

Query: 221 CSLDSIFKVGFGVDLDCLEEPSKAGS--GFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGS 280
           CSLDSIFKVGFGVDL+C+EEPSKA    GFM+AFD++SAQVFWR+IDPFWKLKRFLN+GS
Sbjct: 184 CSLDSIFKVGFGVDLNCVEEPSKAAGRRGFMEAFDNASAQVFWRFIDPFWKLKRFLNVGS 243

Query: 281 EASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLK 340
           EASFRNN+K+IDAFVHQLIS RRKLL QPNL                             
Sbjct: 244 EASFRNNLKIIDAFVHQLISARRKLLHQPNL----------------------------- 303

Query: 341 KNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQ 400
           K DKEDILSRFLMESEKDPTRMND+YLRDIVLNFMLAG+DTSAGTLSWFFYMLCKNPLIQ
Sbjct: 304 KIDKEDILSRFLMESEKDPTRMNDQYLRDIVLNFMLAGRDTSAGTLSWFFYMLCKNPLIQ 363

Query: 401 EKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAE 460
           EKVAEEV QIVGVQGEETDI+LF+QNLTDSALDKMHYLHAAL ETLRLYPAVPVDGRTAE
Sbjct: 364 EKVAEEVSQIVGVQGEETDIHLFIQNLTDSALDKMHYLHAALTETLRLYPAVPVDGRTAE 423

Query: 461 IDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAF 520
           IDDILP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAF
Sbjct: 424 IDDILPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAF 483

Query: 521 HAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAIPR 580
           HAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRN+TYRIMLTLHIDGGLPL+A+PR
Sbjct: 484 HAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNVTYRIMLTLHIDGGLPLLALPR 514

Query: 581 IRK 582
           +RK
Sbjct: 544 VRK 514

BLAST of Spg031830 vs. ExPASy TrEMBL
Match: A0A5A7U692 (Cytochrome P450 704C1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004140 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 3.0e-257
Identity = 445/533 (83.49%), Postives = 473/533 (88.74%), Query Frame = 0

Query: 39  MEVNFNIFTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNL 98
           MEVNFNI T  ++ L    LF LS FILLL+TLAGKSIT+SDYSPVYGT YGQAFY NNL
Sbjct: 1   MEVNFNIITFFTVVL---CLFFLSFFILLLKTLAGKSITNSDYSPVYGTIYGQAFYLNNL 60

Query: 99  YDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEG 158
           YDHLTAVAKRHRTFRLL  +YSEIYTVDPRNVEH+LKTKF+ Y KGSKDQE+CGDLFGEG
Sbjct: 61  YDHLTAVAKRHRTFRLLGESYSEIYTVDPRNVEHILKTKFENYRKGSKDQEVCGDLFGEG 120

Query: 159 IFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLL 218
           IFAVDGEKWK+QRKLASYEFST++LRDFSCSVFRRNA KLVG++S+ ST  RVFD+QDLL
Sbjct: 121 IFAVDGEKWKEQRKLASYEFSTKILRDFSCSVFRRNAEKLVGIVSEFSTMARVFDVQDLL 180

Query: 219 MRCSLDSIFKVGFGVDLDCLEEPSKAGS--GFMKAFDDSSAQVFWRYIDPFWKLKRFLNI 278
           MRCSLDSIFKVGFGVDL+C+EEPSKA    GFM+AFDD+SAQVFWRYIDPFWKLKRFLNI
Sbjct: 181 MRCSLDSIFKVGFGVDLNCMEEPSKAAGRRGFMEAFDDASAQVFWRYIDPFWKLKRFLNI 240

Query: 279 GSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLN 338
           GSEASFRNN+K+IDAFVHQLIS RRKLL QPNL                           
Sbjct: 241 GSEASFRNNLKIIDAFVHQLISARRKLLHQPNL--------------------------- 300

Query: 339 LKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPL 398
             K DKEDILSRFLMESEKDPTRMND+YLRDIVLNFMLAG+DTSAGTLSWFFYMLCKNPL
Sbjct: 301 --KIDKEDILSRFLMESEKDPTRMNDQYLRDIVLNFMLAGRDTSAGTLSWFFYMLCKNPL 360

Query: 399 IQEKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRT 458
           IQEKVAEEV QIVGVQGEETDINLFVQNLTDSALDKMHYLHAAL ETLRLYPAVP+DGRT
Sbjct: 361 IQEKVAEEVSQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALTETLRLYPAVPIDGRT 420

Query: 459 AEIDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 518
           AE DDILP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI
Sbjct: 421 AETDDILPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 480

Query: 519 AFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDG 570
           +FHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRN+TYRIMLTLHIDG
Sbjct: 481 SFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNVTYRIMLTLHIDG 501

BLAST of Spg031830 vs. ExPASy TrEMBL
Match: A0A6J1CZU0 (cytochrome P450 704C1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015998 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.2e-247
Identity = 430/545 (78.90%), Postives = 470/545 (86.24%), Query Frame = 0

Query: 39  MEVN-FNI-FTVVSIALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFN 98
           MEVN  NI FTVVS  L T  LF L   I+LLRTLAGKSIT+S+Y+PVYG+ YGQA Y  
Sbjct: 1   MEVNIINIFFTVVSFILFTSFLFFL---IVLLRTLAGKSITNSEYAPVYGSVYGQALYLK 60

Query: 99  NLYDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFG 158
            L+DHLT VAK+H TFRL+A+AYSEIYT+DPRN+EH+LKTKFDKY KGS +Q + GDLFG
Sbjct: 61  KLFDHLTEVAKKHGTFRLVAAAYSEIYTIDPRNIEHILKTKFDKYSKGSNNQAVVGDLFG 120

Query: 159 EGIFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQD 218
           EGIFAVDGEKWKQQRKLASYEFSTR+LRDFSCSVFRRNAAKLVG++SQ +  GR FDIQD
Sbjct: 121 EGIFAVDGEKWKQQRKLASYEFSTRILRDFSCSVFRRNAAKLVGVVSQFAAMGRPFDIQD 180

Query: 219 LLMRCSLDSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNI 278
           LLMR +LDSIFKVGFGV+L+CLEE SK G GFMKAFDDSSAQ+FWRYIDP WKLKRFLNI
Sbjct: 181 LLMRSALDSIFKVGFGVELNCLEESSKEGIGFMKAFDDSSAQIFWRYIDPLWKLKRFLNI 240

Query: 279 GSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLN 338
           GSE S RNN+K IDAFVHQLI D+RKLLQQPNL                           
Sbjct: 241 GSEGSLRNNIKTIDAFVHQLIRDKRKLLQQPNL--------------------------- 300

Query: 339 LKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPL 398
             KNDKEDIL RFLMESEKDPTRMND+YLRDIVLNFMLAGKD+S GTLSWFFYMLCKNPL
Sbjct: 301 --KNDKEDILWRFLMESEKDPTRMNDQYLRDIVLNFMLAGKDSSGGTLSWFFYMLCKNPL 360

Query: 399 IQEKVAEEVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRT 458
           IQEK+AEEV+Q+VG++GEE DINLF++NLTDSALDKMHYLHAAL ETLRLYP VPVDGRT
Sbjct: 361 IQEKIAEEVKQVVGIEGEEADINLFIENLTDSALDKMHYLHAALTETLRLYPPVPVDGRT 420

Query: 459 AEIDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 518
           AEIDDILP+G+KLRKGD VYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI
Sbjct: 421 AEIDDILPDGYKLRKGDGVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFI 480

Query: 519 AFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAI 578
           AFHAGPRMCLGK+FAYRQMKIVSA+LLQFFRFKL D TRN+TYRIMLTLHIDGGLPL+A+
Sbjct: 481 AFHAGPRMCLGKEFAYRQMKIVSASLLQFFRFKLGDATRNVTYRIMLTLHIDGGLPLLAL 513

Query: 579 PRIRK 582
           PR  K
Sbjct: 541 PRKTK 513

BLAST of Spg031830 vs. TAIR 10
Match: AT2G45510.1 (cytochrome P450, family 704, subfamily A, polypeptide 2 )

HSP 1 Score: 630.9 bits (1626), Expect = 9.9e-181
Identity = 308/535 (57.57%), Postives = 391/535 (73.08%), Query Frame = 0

Query: 45  IFTVVSIALLTVSLFLLSLFI-LLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNLYDHLT 104
           I T ++I + T    +L   I L++R   GKS     Y+PV+ T +   F+ + LYD+ T
Sbjct: 3   ILTSIAITVATTIFIVLCFTIYLMIRIFTGKSRNDKRYAPVHATVFDLLFHSDELYDYET 62

Query: 105 AVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEGIFAVD 164
            +A+   T+R L+   SEI T DPRNVEH+LKT+FD Y KG   +E   DL G GIFAVD
Sbjct: 63  EIAREKPTYRFLSPGQSEILTADPRNVEHILKTRFDNYSKGHSSRENMADLLGHGIFAVD 122

Query: 165 GEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLLMRCSL 224
           GEKW+QQRKL+S+EFSTRVLRDFSCSVFRRNA+KLVG +S+ + SG+ FD QDLLMRC+L
Sbjct: 123 GEKWRQQRKLSSFEFSTRVLRDFSCSVFRRNASKLVGFVSEFALSGKAFDAQDLLMRCTL 182

Query: 225 DSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGSEASFR 284
           DSIFKVGFGV+L CL+  SK G  FM+AFD+ +     R+IDP WKLK F NIGS++  +
Sbjct: 183 DSIFKVGFGVELKCLDGFSKEGQEFMEAFDEGNVATSSRFIDPLWKLKWFFNIGSQSKLK 242

Query: 285 NNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLKKNDKE 344
            ++  ID FV+ LI+ +R                       KE+    N V+      +E
Sbjct: 243 KSIATIDKFVYSLITTKR-----------------------KELAKEQNTVV------RE 302

Query: 345 DILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQEKVAE 404
           DILSRFL+ESEKDP  MND+YLRDI+LNFM+AGKDT+A  LSWF YMLCKNPL+QEK+ +
Sbjct: 303 DILSRFLVESEKDPENMNDKYLRDIILNFMIAGKDTTAALLSWFLYMLCKNPLVQEKIVQ 362

Query: 405 EVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAEIDDIL 464
           E+R +     + TD+N FV+++ + ALD+MHYLHAAL+ETLRLYP VPVD R AE DD+L
Sbjct: 363 EIRDVTFSHEKTTDVNGFVESINEEALDEMHYLHAALSETLRLYPPVPVDMRCAENDDVL 422

Query: 465 PNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAFHAGPR 524
           P+GH++ KGD++YY+AY+MGRM  +WG+DAE+FKPERWL++G F+PESPFKFI+FHAGPR
Sbjct: 423 PDGHRVSKGDNIYYIAYAMGRMTYIWGQDAEEFKPERWLKDGLFQPESPFKFISFHAGPR 482

Query: 525 MCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAIPR 579
           +CLGKDFAYRQMKIVS ALL FFRFK+AD    + Y+ MLTLH+DGGL L AIPR
Sbjct: 483 ICLGKDFAYRQMKIVSMALLHFFRFKMADENSKVYYKRMLTLHVDGGLHLCAIPR 508

BLAST of Spg031830 vs. TAIR 10
Match: AT2G44890.1 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 599.0 bits (1543), Expect = 4.2e-171
Identity = 292/528 (55.30%), Postives = 380/528 (71.97%), Query Frame = 0

Query: 51  IALLTVSLFLLSLFILLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNLYDHLTAVAKRHR 110
           I + T+ + L     L +R   GKS     Y+PV+ T +   F+ + LYD+ T +A+   
Sbjct: 4   IVVTTIFILLSFALYLTIRIFTGKSRNDKRYTPVHATIFDLFFHSHKLYDYETEIARTKP 63

Query: 111 TFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEGIFAVDGEKWKQQ 170
           TFR L+   SEI+T DPRNVEH+LKT+F  Y KG        DL G GIFAVDGEKWKQQ
Sbjct: 64  TFRFLSPGQSEIFTADPRNVEHILKTRFHNYSKGPVGTVNLADLLGHGIFAVDGEKWKQQ 123

Query: 171 RKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLLMRCSLDSIFKVG 230
           RKL S+EFSTRVLR+FS SVFR +A+KLVG +++ + SG+ FD QD+LM+C+LDSIFKVG
Sbjct: 124 RKLVSFEFSTRVLRNFSYSVFRTSASKLVGFIAEFALSGKSFDFQDMLMKCTLDSIFKVG 183

Query: 231 FGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGSEASFRNNVKVID 290
           FGV+L CL+  SK G  FMKAFD+ +     R  DPFWKLK FLNIGSE+  + ++ +ID
Sbjct: 184 FGVELGCLDGFSKEGEEFMKAFDEGNGATSSRVTDPFWKLKCFLNIGSESRLKKSIAIID 243

Query: 291 AFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLKKNDKEDILSRFL 350
            FV+ LI+ +RK L                    KE  +S+          +EDILS+FL
Sbjct: 244 KFVYSLITTKRKELS-------------------KEQNTSV----------REDILSKFL 303

Query: 351 MESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQEKVAEEVRQIVG 410
           +ESEKDP  MND+YLRDI+LN M+AGKDT+A +LSWF YMLCKNPL+QEK+ +E+R +  
Sbjct: 304 LESEKDPENMNDKYLRDIILNVMVAGKDTTAASLSWFLYMLCKNPLVQEKIVQEIRDVTS 363

Query: 411 VQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAEIDDILPNGHKLR 470
              + TD+N F++++T+ AL +M YLHAAL+ET+RLYP VP   R AE DD+LP+GH++ 
Sbjct: 364 SHEKTTDVNGFIESVTEEALAQMQYLHAALSETMRLYPPVPEHMRCAENDDVLPDGHRVS 423

Query: 471 KGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAFHAGPRMCLGKDF 530
           KGD++YY++Y+MGRM  +WG+DAE+FKPERWL++G F+PES FKFI+FHAGPR+C+GKDF
Sbjct: 424 KGDNIYYISYAMGRMTYIWGQDAEEFKPERWLKDGVFQPESQFKFISFHAGPRICIGKDF 483

Query: 531 AYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAIPR 579
           AYRQMKIVS ALL FFRFK+AD    ++Y+ MLTLH+DGGL L AIPR
Sbjct: 484 AYRQMKIVSMALLHFFRFKMADENSKVSYKKMLTLHVDGGLHLCAIPR 502

BLAST of Spg031830 vs. TAIR 10
Match: AT2G44890.2 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 567.0 bits (1460), Expect = 1.8e-161
Identity = 283/535 (52.90%), Postives = 368/535 (68.79%), Query Frame = 0

Query: 45  IFTVVSIALLTVSLFLLSLFI-LLLRTLAGKSITSSDYSPVYGTTYGQAFYFNNLYDHLT 104
           I T ++I ++T    LLS  + L +R   GKS     Y+PV+ T +   F+ + LYD+ T
Sbjct: 3   ILTSMAIIVVTTIFILLSFALYLTIRIFTGKSRNDKRYTPVHATIFDLFFHSHKLYDYET 62

Query: 105 AVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYPKGSKDQELCGDLFGEGIFAVD 164
            +A+   TFR L+   SEI+T DPRNVEH+LKT+F  Y KG        DL G GIFAVD
Sbjct: 63  EIARTKPTFRFLSPGQSEIFTADPRNVEHILKTRFHNYSKGPVGTVNLADLLGHGIFAVD 122

Query: 165 GEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRVFDIQDLLMRCSL 224
           GEKWKQQRKL S+EFSTRVLR+FS SVFR +A+KLVG +++ + SG+ FD QD+LM+C+L
Sbjct: 123 GEKWKQQRKLVSFEFSTRVLRNFSYSVFRTSASKLVGFIAEFALSGKSFDFQDMLMKCTL 182

Query: 225 DSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWRYIDPFWKLKRFLNIGSEASFR 284
           DSIFKVGFGV+L CL+  SK G  FMKAFD+ +     R  DPFWKLK FLNIGSE+  +
Sbjct: 183 DSIFKVGFGVELGCLDGFSKEGEEFMKAFDEGNGATSSRVTDPFWKLKCFLNIGSESRLK 242

Query: 285 NNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIISSLNFVLNLKKNDKE 344
            ++ +ID FV+ LI+ +RK                                  L K    
Sbjct: 243 KSIAIIDKFVYSLITTKRK---------------------------------ELSKEQNT 302

Query: 345 DILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFYMLCKNPLIQEKVAE 404
           DILS+FL+ESEKDP  MND+YLRDI+LN M+AGKDT+A +LSWF YMLCKNPL+QEK+ +
Sbjct: 303 DILSKFLLESEKDPENMNDKYLRDIILNVMVAGKDTTAASLSWFLYMLCKNPLVQEKIVQ 362

Query: 405 EVRQIVGVQGEETDINLFVQNLTDSALDKMHYLHAALAETLRLYPAVPVDGRTAEIDDIL 464
           E+R +     + TD+N F++++T+ AL +M YLHAAL+ET+RLYP VP   R AE DD+L
Sbjct: 363 EIRDVTSSHEKTTDVNGFIESVTEEALAQMQYLHAALSETMRLYPPVPEHMRCAENDDVL 422

Query: 465 PNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGTFRPESPFKFIAFHAGPR 524
           P+GH++ KGD++YY++Y+MGRM  +WG+DAE+FKPERWL++G              AGPR
Sbjct: 423 PDGHRVSKGDNIYYISYAMGRMTYIWGQDAEEFKPERWLKDG--------------AGPR 482

Query: 525 MCLGKDFAYRQMKIVSAALLQFFRFKLADPTRNITYRIMLTLHIDGGLPLVAIPR 579
           +C+GKDFAYRQMKIVS ALL FFRFK+AD    ++Y+ MLTLH+DGGL L AIPR
Sbjct: 483 ICIGKDFAYRQMKIVSMALLHFFRFKMADENSKVSYKKMLTLHVDGGLHLCAIPR 490

BLAST of Spg031830 vs. TAIR 10
Match: AT1G69500.1 (cytochrome P450, family 704, subfamily B, polypeptide 1 )

HSP 1 Score: 386.3 bits (991), Expect = 4.3e-107
Identity = 219/513 (42.69%), Postives = 296/513 (57.70%), Query Frame = 0

Query: 83  PVYGTTYGQAFYFNNLYDHLTAVAKRHRTFRLLASAYSEIYTVDPRNVEHVLKTKFDKYP 142
           P+ G    Q   F+ ++D L       RT  +     +  Y  DP NVE+VLKT F  YP
Sbjct: 32  PLVGAAIEQLTNFDRMHDWLVEYLYNSRTVVVPMPFTTYTYIADPINVEYVLKTNFSNYP 91

Query: 143 KGSKDQELCGDLFGEGIFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLL 202
           KG         L G+GIF  DGE W++QRK AS+EF+++ LRDFS  VF+  + KL  +L
Sbjct: 92  KGETYHSYMEVLLGDGIFNSDGELWRKQRKTASFEFASKNLRDFSTVVFKEYSLKLFTIL 151

Query: 203 SQLSTSGRVFDIQDLLMRCSLDSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWR 262
           SQ S   +  D+Q+LLMR +LDSI KVGFGV++  L  P    + F KAFD ++  V  R
Sbjct: 152 SQASFKEQQVDMQELLMRMTLDSICKVGFGVEIGTL-APELPENHFAKAFDTANIIVTLR 211

Query: 263 YIDPFWKLKRFLNIGSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLT 322
           +IDP WK+K+FLNIGSEA    ++KV++ F + +I  R+  L +                
Sbjct: 212 FIDPLWKMKKFLNIGSEALLGKSIKVVNDFTYSVIRRRKAELLEAQ-------------- 271

Query: 323 GVKEIISSLNFVLNLKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAG 382
                IS  N   N     K DILSRF+  S+   ++  ++ LRDIVLNF++AG+DT+A 
Sbjct: 272 -----ISPTNNNNNNNNKVKHDILSRFIEISDDPDSKETEKSLRDIVLNFVIAGRDTTAT 331

Query: 383 TLSWFFYMLCKNPLIQEKVAEEVRQIVGVQGEET----------DINLFVQNLTDSA--- 442
           TL+W  YM+  N  + EK+  E++++     E T          D N F + +T+ A   
Sbjct: 332 TLTWAIYMIMMNENVAEKLYSELQELEKESAEATNTSLHQYDTEDFNSFNEKVTEFAGLL 391

Query: 443 ----LDKMHYLHAALAETLRLYPAVPVDGRTAEIDDILPNGHKLRKGDSVYYLAYSMGRM 502
               L K+HYLHA + ETLRLYPAVP D +    DD+LPNG K++ G  V Y+ YSMGRM
Sbjct: 392 NYDSLGKLHYLHAVITETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMVTYVPYSMGRM 451

Query: 503 PCLWGEDAEDFKPERWLENGTFRPESPFKFIAFHAGPRMCLGKDFAYRQMKIVSAALLQF 562
              WG DA  FKPERWL++G F+  SPFKF AF AGPR+CLGKD AY QMK+  A L +F
Sbjct: 452 EYNWGSDAALFKPERWLKDGVFQNASPFKFTAFQAGPRICLGKDSAYLQMKMAMAILCRF 511

Query: 563 FRFKLADPTRNITYRIMLTLHIDGGLPLVAIPR 579
           ++F L  P   + YR+M  L +  GL +    R
Sbjct: 512 YKFHLV-PNHPVKYRMMTILSMAHGLKVTVSRR 523

BLAST of Spg031830 vs. TAIR 10
Match: AT5G08250.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 2.1e-90
Identity = 184/493 (37.32%), Postives = 285/493 (57.81%), Query Frame = 0

Query: 96  NNLYDHLTAV-AKRHRTFRLLASAYSE---IYTVDPRNVEHVLKTKFDKYPKGSKDQELC 155
           +N+Y+ L+ V   ++ TFR     +S    + T DPRNVEH+LKT+F  YPKGS  +E  
Sbjct: 19  SNIYEWLSDVLISQNGTFRFRGPWFSTLNCVVTCDPRNVEHLLKTRFSIYPKGSYFRETM 78

Query: 156 GDLFGEGIFAVDGEKWKQQRKLASYEFSTRVLRDFSCSVFRRNAAKLVGLLSQLSTSGRV 215
            DL G+GIF  D   W++QRK AS EF +   R  +             LL  L TSG++
Sbjct: 79  QDLLGDGIFNTDDGTWQRQRKAASVEFHSAKFRQLTSQSLHELVHN--RLLPVLETSGKI 138

Query: 216 FDIQDLLMRCSLDSIFKVGFGVDLDCLEEPSKAGSGFMKAFDDSSAQVFWRYIDP--FWK 275
            D+QD+L+R + D++  + FGVD  CL  P      F KAF+D++     R++ P   WK
Sbjct: 139 -DLQDILLRLTFDNVCMIAFGVDPGCL-SPKLPEIPFAKAFEDATEATVVRFVMPKFVWK 198

Query: 276 LKRFLNIGSEASFRNNVKVIDAFVHQLISDRRKLLQQPNLHCSLSQLDFAVLTGVKEIIS 335
           L R LN+G+E   + ++  +D F  ++I  R+K +                         
Sbjct: 199 LMRSLNLGTEKKLKESINGVDDFAEEVIRTRKKEMS------------------------ 258

Query: 336 SLNFVLNLKKNDKEDILSRFLMESEKDPTRMNDRYLRDIVLNFMLAGKDTSAGTLSWFFY 395
                L  +   + D+L+ F+   +++  + +D++LRDI +NF+LAG+DTS+  LSWFF+
Sbjct: 259 -----LETEIAKRPDLLTIFMGLRDENGQKFSDKFLRDICVNFILAGRDTSSVALSWFFW 318

Query: 396 MLCKNPLIQEKVAEEVRQIV--GVQGEETDINL-FVQNLTDSALDKMHYLHAALAETLRL 455
           ++ KNP ++EK+   + +I+   V   +T  N+ +        + KM YL AAL+ETLRL
Sbjct: 319 LIEKNPEVEEKIMMGICKILEQRVDHGDTKKNMEYEPVFRPEEIKKMDYLQAALSETLRL 378

Query: 456 YPAVPVDGRTAEIDDILPNGHKLRKGDSVYYLAYSMGRMPCLWGEDAEDFKPERWLENGT 515
           YP+VPVD +    DD+ P+G KL+KG+ V Y  Y+MGRM  +WG+D  +FKPERWL +G 
Sbjct: 379 YPSVPVDHKEVLEDDVFPDGTKLKKGEKVIYAIYAMGRMETIWGKDCREFKPERWLRDGR 438

Query: 516 FRPESPFKFIAFHAGPRMCLGKDFAYRQMKIVSAALLQFFRFKLADP-TRNITYRIMLTL 575
           +  ES +KF AF+ GPR+CLGKDFAY QM+ V+AA++  ++ ++ D     +  ++ LT+
Sbjct: 439 YMSESAYKFTAFNGGPRLCLGKDFAYYQMRYVAAAIIYRYKVRVDDKGGHKVEPKMALTM 478

Query: 576 HIDGGLPLVAIPR 579
           ++  GL +  + R
Sbjct: 499 YMKHGLKVNMVKR 478

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877606.12.8e-26282.84cytochrome P450 704C1-like isoform X1 [Benincasa hispida][more]
XP_008438136.18.2e-26282.57PREDICTED: cytochrome P450 704C1-like isoform X1 [Cucumis melo][more]
XP_038877612.11.4e-26182.66cytochrome P450 704C1-like isoform X2 [Benincasa hispida][more]
XP_008438137.14.1e-26182.39PREDICTED: cytochrome P450 704C1-like isoform X2 [Cucumis melo][more]
XP_004133900.11.5e-25882.14cytochrome P450 704C1 isoform X1 [Cucumis sativus] >KGN56579.1 hypothetical prot... [more]
Match NameE-valueIdentityDescription
Q50EK33.0e-13447.83Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1[more]
Q9C7886.0e-10642.69Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1[more]
Q9FMY11.8e-8634.33Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1[more]
Q9CAD61.6e-8237.36Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
Q9ZUX12.3e-8135.75Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AW914.0e-26282.57cytochrome P450 704C1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483335 PE... [more]
A0A1S3AVB32.0e-26182.39cytochrome P450 704C1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483335 PE... [more]
A0A0A0L6D67.0e-25982.14Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G124970 PE=3 SV=1[more]
A0A5A7U6923.0e-25783.49Cytochrome P450 704C1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1CZU01.2e-24778.90cytochrome P450 704C1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
Match NameE-valueIdentityDescription
AT2G45510.19.9e-18157.57cytochrome P450, family 704, subfamily A, polypeptide 2 [more]
AT2G44890.14.2e-17155.30cytochrome P450, family 704, subfamily A, polypeptide 1 [more]
AT2G44890.21.8e-16152.90cytochrome P450, family 704, subfamily A, polypeptide 1 [more]
AT1G69500.14.3e-10742.69cytochrome P450, family 704, subfamily B, polypeptide 1 [more]
AT5G08250.12.1e-9037.32Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 438..449
score: 43.75
coord: 375..392
score: 38.26
coord: 525..536
score: 35.31
coord: 516..525
score: 49.19
IPR001128Cytochrome P450PFAMPF00067p450coord: 95..554
e-value: 1.4E-73
score: 248.3
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 384..410
score: 33.99
coord: 525..548
score: 29.73
coord: 364..381
score: 29.38
coord: 437..455
score: 28.9
coord: 515..525
score: 46.73
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 90..578
e-value: 9.1E-108
score: 362.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 91..578
NoneNo IPR availablePANTHERPTHR24296:SF163CYTOCHROME P450, FAMILY 704, SUBFAMILY A, POLYPEPTIDE 1coord: 47..580
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 47..580
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 518..527

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031830.1Spg031830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0004672 protein kinase activity