Spg031794 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031794
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionadenylate isopentenyltransferase 5, chloroplastic
Locationscaffold11: 38888511 .. 38889485 (-)
RNA-Seq ExpressionSpg031794
SyntenySpg031794
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTGTCATTATCCTCCGCCGCCGGCAAGCAAGTTCAGCAGCTGGTCGGCAATTTTCAGGGGAGTTTCAACATGGAGCAATTCTTCCGGCGGAAAGATAAAGTGGTTGTTGTAATGGGGGCGACCGGAACGGGTAAATCCCGGTTGGCGATTGATTTAGCTACTCGATTTCCGGCGGAAATCGTAAACTCCGATAAGATTCAAGTCTTCGAAGGACTCGATGTTGTCACTAACAAAGTCACCGACGAAGAACGCCGTGGAATTCCTCACCATTTGTTGGGCTCGATCGACCCAGATTCAAATTTCTCCTCTGATGATTTTAACCACCATGCCTCTGTTGCAATCGAATCGATTTTGGCTCGAGATCGCCTTCCGATCATCGCCGGCGGCTCGAATTCGTACATTGAAGCTCTGGTTGATGATTATGCAGAGTCCCGATTTCGATATGAGTTCTGTTTTTTGTGGGTCGATGTGTCGTTGCCGATTCTTCAGAATTTTGTCTCCGACCGTGTTGATCGGATGGTCGACGGTGGATTCGTCGGAGAAGTGAGGGAGATTTTTGATCCCAAAGGGAATTATTCTCAGGGGATTAAACGGGCCATCGGAGTACCGGAATTACACGACTATTTGAGAGCAGAGCGCGACGGCGCCGACGAGAGGACGTTGACGATTCTTCTCGAATTGGCGATTTCGAAGATTAAAGACAATACTTGCCGATTGGCCCTCCGGCAACTGCAGAAGATTCGCCTCCTTCGGAATAAGTGGAATTGGAATCTCCGCCGTTTGGATGCCACCGGAGCGTTTTTGACCGACGGAGGAAACTCCGACGCCGTGTGGGAAAAAAACGTCGTCGAACCTAGCTCGATGATCGTCGATCAATTTCTCTGCGACGATAGCCGTCGTATTACCACCGCCATTATCTCGCCGGAGCCGGTCCCCCGCGCCGCCGTCGCCGCCGCCGTAAGTCGTTAA

mRNA sequence

ATGGCATTGTCATTATCCTCCGCCGCCGGCAAGCAAGTTCAGCAGCTGGTCGGCAATTTTCAGGGGAGTTTCAACATGGAGCAATTCTTCCGGCGGAAAGATAAAGTGGTTGTTGTAATGGGGGCGACCGGAACGGGTAAATCCCGGTTGGCGATTGATTTAGCTACTCGATTTCCGGCGGAAATCGTAAACTCCGATAAGATTCAAGTCTTCGAAGGACTCGATGTTGTCACTAACAAAGTCACCGACGAAGAACGCCGTGGAATTCCTCACCATTTGTTGGGCTCGATCGACCCAGATTCAAATTTCTCCTCTGATGATTTTAACCACCATGCCTCTGTTGCAATCGAATCGATTTTGGCTCGAGATCGCCTTCCGATCATCGCCGGCGGCTCGAATTCGTACATTGAAGCTCTGGTTGATGATTATGCAGAGTCCCGATTTCGATATGAGTTCTGTTTTTTGTGGGTCGATGTGTCGTTGCCGATTCTTCAGAATTTTGTCTCCGACCGTGTTGATCGGATGGTCGACGGTGGATTCGTCGGAGAAGTGAGGGAGATTTTTGATCCCAAAGGGAATTATTCTCAGGGGATTAAACGGGCCATCGGAGTACCGGAATTACACGACTATTTGAGAGCAGAGCGCGACGGCGCCGACGAGAGGACGTTGACGATTCTTCTCGAATTGGCGATTTCGAAGATTAAAGACAATACTTGCCGATTGGCCCTCCGGCAACTGCAGAAGATTCGCCTCCTTCGGAATAAGTGGAATTGGAATCTCCGCCGTTTGGATGCCACCGGAGCGTTTTTGACCGACGGAGGAAACTCCGACGCCGTGTGGGAAAAAAACGTCGTCGAACCTAGCTCGATGATCGTCGATCAATTTCTCTGCGACGATAGCCGTCGTATTACCACCGCCATTATCTCGCCGGAGCCGGTCCCCCGCGCCGCCGTCGCCGCCGCCGTAAGTCGTTAA

Coding sequence (CDS)

ATGGCATTGTCATTATCCTCCGCCGCCGGCAAGCAAGTTCAGCAGCTGGTCGGCAATTTTCAGGGGAGTTTCAACATGGAGCAATTCTTCCGGCGGAAAGATAAAGTGGTTGTTGTAATGGGGGCGACCGGAACGGGTAAATCCCGGTTGGCGATTGATTTAGCTACTCGATTTCCGGCGGAAATCGTAAACTCCGATAAGATTCAAGTCTTCGAAGGACTCGATGTTGTCACTAACAAAGTCACCGACGAAGAACGCCGTGGAATTCCTCACCATTTGTTGGGCTCGATCGACCCAGATTCAAATTTCTCCTCTGATGATTTTAACCACCATGCCTCTGTTGCAATCGAATCGATTTTGGCTCGAGATCGCCTTCCGATCATCGCCGGCGGCTCGAATTCGTACATTGAAGCTCTGGTTGATGATTATGCAGAGTCCCGATTTCGATATGAGTTCTGTTTTTTGTGGGTCGATGTGTCGTTGCCGATTCTTCAGAATTTTGTCTCCGACCGTGTTGATCGGATGGTCGACGGTGGATTCGTCGGAGAAGTGAGGGAGATTTTTGATCCCAAAGGGAATTATTCTCAGGGGATTAAACGGGCCATCGGAGTACCGGAATTACACGACTATTTGAGAGCAGAGCGCGACGGCGCCGACGAGAGGACGTTGACGATTCTTCTCGAATTGGCGATTTCGAAGATTAAAGACAATACTTGCCGATTGGCCCTCCGGCAACTGCAGAAGATTCGCCTCCTTCGGAATAAGTGGAATTGGAATCTCCGCCGTTTGGATGCCACCGGAGCGTTTTTGACCGACGGAGGAAACTCCGACGCCGTGTGGGAAAAAAACGTCGTCGAACCTAGCTCGATGATCGTCGATCAATTTCTCTGCGACGATAGCCGTCGTATTACCACCGCCATTATCTCGCCGGAGCCGGTCCCCCGCGCCGCCGTCGCCGCCGCCGTAAGTCGTTAA

Protein sequence

MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESILARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGFVGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCRLALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDSRRITTAIISPEPVPRAAVAAAVSR
Homology
BLAST of Spg031794 vs. NCBI nr
Match: XP_038898938.1 (adenylate isopentenyltransferase 5, chloroplastic [Benincasa hispida])

HSP 1 Score: 572.4 bits (1474), Expect = 2.5e-159
Identity = 292/324 (90.12%), Postives = 304/324 (93.83%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           MALSLSSAAGKQVQ LVGNFQG+FNMEQFFRRK+KVVVVMGATGTGKSRLAIDLATRFPA
Sbjct: 3   MALSLSSAAGKQVQPLVGNFQGNFNMEQFFRRKEKVVVVMGATGTGKSRLAIDLATRFPA 62

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVNSDKIQV++GLDVVTNKVTDEERRGI HHLL SIDP+SNFSS DF  HASVAIESIL
Sbjct: 63  EIVNSDKIQVYKGLDVVTNKVTDEERRGISHHLLDSIDPNSNFSSHDFTRHASVAIESIL 122

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQ FVSDRVDRMVDGGF
Sbjct: 123 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGF 182

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           VGEVR+IFDP+G+YS GIKRAIGVPELHDYLRAE DGADER LTILLELAISKIKDNTCR
Sbjct: 183 VGEVRQIFDPEGDYSYGIKRAIGVPELHDYLRAEHDGADERILTILLELAISKIKDNTCR 242

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LALRQL+KIR LRNKWNWNLRRLDATGAFLTDGGNSD  W+K V+EPSS IVDQFLCD +
Sbjct: 243 LALRQLEKIRRLRNKWNWNLRRLDATGAFLTDGGNSDDAWQKLVLEPSSRIVDQFLCDGN 302

Query: 301 RRITTAIISPEPVPRAAVAAAVSR 325
           RRITTAI SPEPVPRA   AAVSR
Sbjct: 303 RRITTAIASPEPVPRA--VAAVSR 324

BLAST of Spg031794 vs. NCBI nr
Match: KAA0049402.1 (adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK17157.1 adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa])

HSP 1 Score: 552.4 bits (1422), Expect = 2.7e-153
Identity = 282/324 (87.04%), Postives = 299/324 (92.28%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           MALSL+SAAGKQVQ LVGNFQG+FNMEQFFRRKDKVVVVMGATGTGKSRLAI+LATRFP+
Sbjct: 15  MALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRFPS 74

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVNSDKIQV+EGLDVVTNKVT+EERRGIPHHLL SIDP SNFSS DF HHASVAIESIL
Sbjct: 75  EIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASVAIESIL 134

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQ FVSDRVDRMVDGGF
Sbjct: 135 ARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGF 194

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           V EVR+IFDP+G+YSQGIKRAIGVPELH+YLRAERDGADER L ILLELAIS+IKDNTCR
Sbjct: 195 VEEVRQIFDPEGDYSQGIKRAIGVPELHEYLRAERDGADERVLKILLELAISRIKDNTCR 254

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LA RQL+KIRLL  KWNWNLRRLDATGAFLTDG NS  VWEK V+EPSS IVDQFLCD S
Sbjct: 255 LAFRQLEKIRLLHTKWNWNLRRLDATGAFLTDGENSVDVWEKLVLEPSSRIVDQFLCDGS 314

Query: 301 RRITTAIISPEPVPRAAVAAAVSR 325
           RRITT I +P+ V RA   AAVSR
Sbjct: 315 RRITTGISTPDAVSRA--VAAVSR 336

BLAST of Spg031794 vs. NCBI nr
Match: XP_008438727.1 (PREDICTED: adenylate isopentenyltransferase 5, chloroplastic [Cucumis melo])

HSP 1 Score: 552.4 bits (1422), Expect = 2.7e-153
Identity = 282/324 (87.04%), Postives = 299/324 (92.28%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           MALSL+SAAGKQVQ LVGNFQG+FNMEQFFRRKDKVVVVMGATGTGKSRLAI+LATRFP+
Sbjct: 3   MALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRFPS 62

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVNSDKIQV+EGLDVVTNKVT+EERRGIPHHLL SIDP SNFSS DF HHASVAIESIL
Sbjct: 63  EIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASVAIESIL 122

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQ FVSDRVDRMVDGGF
Sbjct: 123 ARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGF 182

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           V EVR+IFDP+G+YSQGIKRAIGVPELH+YLRAERDGADER L ILLELAIS+IKDNTCR
Sbjct: 183 VEEVRQIFDPEGDYSQGIKRAIGVPELHEYLRAERDGADERVLKILLELAISRIKDNTCR 242

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LA RQL+KIRLL  KWNWNLRRLDATGAFLTDG NS  VWEK V+EPSS IVDQFLCD S
Sbjct: 243 LAFRQLEKIRLLHTKWNWNLRRLDATGAFLTDGENSVDVWEKLVLEPSSRIVDQFLCDGS 302

Query: 301 RRITTAIISPEPVPRAAVAAAVSR 325
           RRITT I +P+ V RA   AAVSR
Sbjct: 303 RRITTGISTPDAVSRA--VAAVSR 324

BLAST of Spg031794 vs. NCBI nr
Match: XP_011650995.1 (adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus] >KGN57030.1 hypothetical protein Csa_010976 [Cucumis sativus])

HSP 1 Score: 546.6 bits (1407), Expect = 1.5e-151
Identity = 279/324 (86.11%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           MALSL+SAAGKQVQ LVGNFQG+FNMEQFFRRKDKVVVVMGATGTGKSRLAI+LATRFP+
Sbjct: 3   MALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRFPS 62

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVNSDKIQV+EGLDVVTNKVT+EERRGIPHHLL SIDP SNFSS DF HHAS AIESIL
Sbjct: 63  EIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASGAIESIL 122

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQ FVSDRVDRMVDGGF
Sbjct: 123 ARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGF 182

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           V EVR+IFDP+G+YSQGIKRAIGVPELH++LRAERDGADER L ILLELAIS+IKDNTCR
Sbjct: 183 VEEVRQIFDPEGDYSQGIKRAIGVPELHEFLRAERDGADERVLNILLELAISRIKDNTCR 242

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LA RQL+KIRLLR+KWNWNLRRLDATG  LTDG NS  VWEK V+EPSS IVDQFLCD S
Sbjct: 243 LAFRQLEKIRLLRSKWNWNLRRLDATGVILTDGENSLDVWEKLVLEPSSRIVDQFLCDGS 302

Query: 301 RRITTAIISPEPVPRAAVAAAVSR 325
           RRITT I +P+ V RA   AAVSR
Sbjct: 303 RRITTGISTPDAVSRA--VAAVSR 324

BLAST of Spg031794 vs. NCBI nr
Match: KAG7028930.1 (Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 545.4 bits (1404), Expect = 3.2e-151
Identity = 279/324 (86.11%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           ++LS ++AAGK+V  LVGNFQGSFNMEQFFRRKDKVV VMGATGTGKSRLAIDLATRFPA
Sbjct: 19  LSLSSAAAAGKRVAPLVGNFQGSFNMEQFFRRKDKVVFVMGATGTGKSRLAIDLATRFPA 78

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVN+DKIQV+EGLDVVTNKVTDEERRGI HHLLGSIDP SNFSS DF HHAS AIESIL
Sbjct: 79  EIVNADKIQVYEGLDVVTNKVTDEERRGITHHLLGSIDPYSNFSSHDFTHHASDAIESIL 138

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPIIAGGSNSYIEALVDDY+E RFRY+FCFLWVDVSLPIL NFVSDRVD+MVDGGF
Sbjct: 139 ARDRLPIIAGGSNSYIEALVDDYSEFRFRYDFCFLWVDVSLPILHNFVSDRVDQMVDGGF 198

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           VGEVREIFDPKG+YS GIKRAIGVPELHDYL+AERDGADERTL+ILLELAISKIKDNTC 
Sbjct: 199 VGEVREIFDPKGDYSYGIKRAIGVPELHDYLKAERDGADERTLSILLELAISKIKDNTCL 258

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LALRQ QKIR L+NK NWNL+RLDATGAFLT+GGNS  VWEKNV+EPSSMIVD+FL DD+
Sbjct: 259 LALRQWQKIRRLQNKRNWNLQRLDATGAFLTEGGNSHPVWEKNVIEPSSMIVDKFLHDDT 318

Query: 301 RRITTAIISPEPVPRAAVAAAVSR 325
           R ITT I SPEPV R   AAAVSR
Sbjct: 319 RHITTTIFSPEPVHR---AAAVSR 339

BLAST of Spg031794 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 352.4 bits (903), Expect = 5.3e-96
Identity = 190/325 (58.46%), Postives = 232/325 (71.38%), Query Frame = 0

Query: 7   SAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPAEIVNSD 66
           +A  + +Q L  NFQG+     FFRRKDKVV VMGATGTGKSRLAIDLATRFPAEIVNSD
Sbjct: 6   TALRQVIQPLSLNFQGNMVDVPFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSD 65

Query: 67  KIQVFEGLDVVTNKVTDEERRGIPHHLLGSI-DPDSNFSSDDFNHHASVAIESILARDRL 126
           KIQV++GLD+VTNKVT EE  G+PHHLLG++ D   +F+++DF   A  A+ESI+ RDR+
Sbjct: 66  KIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRV 125

Query: 127 PIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGFVGEVR 186
           PIIAGGSNSYIEALV+D  + R RY  CFLWVDVS P+L +FVS+RVD+MVD G V EVR
Sbjct: 126 PIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVR 185

Query: 187 EIFDP-KGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCRLALR 246
            IFDP   +YS GI+RAIGVPEL ++LR+E       T   LLE AI KIK+NTC LA R
Sbjct: 186 RIFDPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACR 245

Query: 247 QLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFL--CDDSRR 306
           QLQKI+ L  +W WN+ R+DAT  FL  G  +D  W+ +V  PS++ V++FL   DD   
Sbjct: 246 QLQKIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFLSYSDDHHL 305

Query: 307 ITTAIISPE---PVPRAAVAAAVSR 325
               I+ PE     P  A  AA+SR
Sbjct: 306 EGANILLPEISAVPPLPAAVAAISR 330

BLAST of Spg031794 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 293.1 bits (749), Expect = 3.8e-78
Identity = 160/300 (53.33%), Postives = 209/300 (69.67%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLV--GNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRF 60
           M  S+SS   KQVQ ++   N     N+  F   K+KV+ VMGATG+GKSRLAIDLATRF
Sbjct: 1   MKFSISSL--KQVQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATRF 60

Query: 61  PAEIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDS-NFSSDDFNHHASVAIE 120
             EI+NSDKIQ+++GLDV+TNKVT +E RG+PHHLLG  D ++ N ++  ++  AS AI 
Sbjct: 61  QGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAIS 120

Query: 121 SILARDRLPIIAGGSNSYIEALVDDYAESRF-RYEFCFLWVDVSLPILQNFVSDRVDRMV 180
            + A ++LPI+AGGSNSYIEALV+  +      Y+ CF+WVDVSLP+L +FVS RVDRM+
Sbjct: 121 KLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMM 180

Query: 181 DGGFVGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKD 240
           + G + EVRE+F+PK NYS GI+RAIGVPELH+YLR E    D  T + +L++A+  IK 
Sbjct: 181 EAGLLEEVREVFNPKANYSVGIRRAIGVPELHEYLRNE-SLVDRATKSKMLDVAVKNIKK 240

Query: 241 NTCRLALRQLQKIRLLRNKWNWNLRRLDATGAFL-TDGGNSDAVWEKNVVEPSSMIVDQF 296
           NT  LA RQL+KI+ L  KW  ++ R+DAT  FL  +    D  WE  V  PS  IVD+F
Sbjct: 241 NTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKF 297

BLAST of Spg031794 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.7e-73
Identity = 156/299 (52.17%), Postives = 204/299 (68.23%), Query Frame = 0

Query: 33  KDKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVFEGLDVVTNKVTDEERRGIPHH 92
           KDKVVV+MGATGTGKSRL++D+ATRF AEI+NSDKIQV +GLD+VTNK+T EE  G+PHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 93  LLGSIDPDSNFSSDDFNHHASVAIESILARDRLPIIAGGSNSYIEALVDDYAES-RFRYE 152
           LLG + P+++ ++ ++ H A+++IES+L R +LPII GGSNSY+EALVDD     R RY+
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYD 159

Query: 153 FCFLWVDVSLPILQNFVSDRVDRMVDGGFVGEVREIFD-PKGNYSQGIKRAIGVPELHDY 212
            CFLWVDV+LP+L  FVS+RVD+MV+ G V EVRE FD    +YS+GIK+AIG PE   +
Sbjct: 160 CCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRF 219

Query: 213 LRAERD-GADERTLTILLELAISKIKDNTCRLALRQLQKIRLLRNKWNWNLRRLDATGAF 272
            R E+    ++R    LL   + +IK NT  LA RQ +KI  LR    W+++R+DAT  F
Sbjct: 220 FRNEQFLNVEDR--EELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVF 279

Query: 273 LTDGGNSDA--VWEKNVVEPSSMIVDQFLCDDSRRITTAIISPEPVPRA--AVAAAVSR 325
                  DA   WE+ V  PS+  V +FL D        I S  P+  A  AVAAA+ R
Sbjct: 280 TKRRSKMDANVAWERLVAGPSTDTVSRFLLD--------IASRRPLVEASTAVAAAMER 328

BLAST of Spg031794 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 3.4e-58
Identity = 127/301 (42.19%), Postives = 175/301 (58.14%), Query Frame = 0

Query: 32  RKDKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVFEGLDVVTNKVTDEERRGIPH 91
           RK+K++V+MGATGTGKSRL+IDLA  FP E++NSDK+QV++GLD+ TNK++  +R G+PH
Sbjct: 28  RKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPH 87

Query: 92  HLLGSIDP-DSNFSSDDFNHHASVAIESILARDRLPIIAGGSNSYIEALVDD-------- 151
           HLLG +DP     +  DF   A  A+  I  R +LP++ GGSNS+I AL+ D        
Sbjct: 88  HLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPG 147

Query: 152 --------YAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGFVGEVREIFDPKGN- 211
                      S  RY+ CFLWVDVS+ +L ++++ RVD M++ G   E+ E + P+   
Sbjct: 148 VFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDED 207

Query: 212 ------YSQGIKRAIGVPELHDYLRAERDG-------ADERTLTILLELAISKIKDNTCR 271
                    G+++AIGVPE   Y    R G         +R      E A+  IK+NTC 
Sbjct: 208 HDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCH 267

Query: 272 LALRQLQKIRLLRNKWNWNLRRLDATGAFLT-----DGGNSDAVWEKNVVEPSSMIVDQF 297
           LA RQ+ KI  L+    W+LRRLDAT +F        G     +WEK V+EPS  IV +F
Sbjct: 268 LAKRQIGKILRLKGA-GWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRF 327

BLAST of Spg031794 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 1.8e-56
Identity = 127/304 (41.78%), Postives = 185/304 (60.86%), Query Frame = 0

Query: 23  SFNMEQ--FFRRKDKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVFEGLDVVTNK 82
           S  MEQ     RKDKVVV++GATG GKSRL++DLATRFP+EI+NSDKIQV+EGL++ TN+
Sbjct: 52  SIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQ 111

Query: 83  VTDEERRGIPHHLLGSIDPD-SNFSSDDFNHHASVAIESILARDRLPIIAGGSNSYIEAL 142
           +T ++RRG+PHHLLG I+P+    ++ +F   AS  ++ I +R ++PIIAGGSNS++ AL
Sbjct: 112 ITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHAL 171

Query: 143 VDDYAESRF--------------RYEFCFLWVDVSLPILQNFVSDRVDRMVDGGFVGEVR 202
           +    + +F              RYE CF+WVDVS  +L  ++  RVD M+D G   E+ 
Sbjct: 172 LAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELS 231

Query: 203 EIFDPKGNYSQ---GIKRAIGVPELHDYLR----AERDGADERTLTILLELAISKIKDNT 262
             +DP  +  +   GI++AIGVPE   Y +     ++    +       + A+  IK NT
Sbjct: 232 RFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNT 291

Query: 263 CRLALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAV------WEKNVVEPSSMIV 297
             LA RQ++KI +L++   W + R+DAT +F      S +       WE+ V+EPS  IV
Sbjct: 292 WTLAKRQVKKIEMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIV 351

BLAST of Spg031794 vs. ExPASy TrEMBL
Match: A0A5A7U0V6 (Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00010 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.3e-153
Identity = 282/324 (87.04%), Postives = 299/324 (92.28%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           MALSL+SAAGKQVQ LVGNFQG+FNMEQFFRRKDKVVVVMGATGTGKSRLAI+LATRFP+
Sbjct: 15  MALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRFPS 74

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVNSDKIQV+EGLDVVTNKVT+EERRGIPHHLL SIDP SNFSS DF HHASVAIESIL
Sbjct: 75  EIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASVAIESIL 134

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQ FVSDRVDRMVDGGF
Sbjct: 135 ARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGF 194

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           V EVR+IFDP+G+YSQGIKRAIGVPELH+YLRAERDGADER L ILLELAIS+IKDNTCR
Sbjct: 195 VEEVRQIFDPEGDYSQGIKRAIGVPELHEYLRAERDGADERVLKILLELAISRIKDNTCR 254

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LA RQL+KIRLL  KWNWNLRRLDATGAFLTDG NS  VWEK V+EPSS IVDQFLCD S
Sbjct: 255 LAFRQLEKIRLLHTKWNWNLRRLDATGAFLTDGENSVDVWEKLVLEPSSRIVDQFLCDGS 314

Query: 301 RRITTAIISPEPVPRAAVAAAVSR 325
           RRITT I +P+ V RA   AAVSR
Sbjct: 315 RRITTGISTPDAVSRA--VAAVSR 336

BLAST of Spg031794 vs. ExPASy TrEMBL
Match: A0A1S3AXR7 (adenylate isopentenyltransferase 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483750 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.3e-153
Identity = 282/324 (87.04%), Postives = 299/324 (92.28%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           MALSL+SAAGKQVQ LVGNFQG+FNMEQFFRRKDKVVVVMGATGTGKSRLAI+LATRFP+
Sbjct: 3   MALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRFPS 62

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVNSDKIQV+EGLDVVTNKVT+EERRGIPHHLL SIDP SNFSS DF HHASVAIESIL
Sbjct: 63  EIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASVAIESIL 122

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQ FVSDRVDRMVDGGF
Sbjct: 123 ARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGF 182

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           V EVR+IFDP+G+YSQGIKRAIGVPELH+YLRAERDGADER L ILLELAIS+IKDNTCR
Sbjct: 183 VEEVRQIFDPEGDYSQGIKRAIGVPELHEYLRAERDGADERVLKILLELAISRIKDNTCR 242

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LA RQL+KIRLL  KWNWNLRRLDATGAFLTDG NS  VWEK V+EPSS IVDQFLCD S
Sbjct: 243 LAFRQLEKIRLLHTKWNWNLRRLDATGAFLTDGENSVDVWEKLVLEPSSRIVDQFLCDGS 302

Query: 301 RRITTAIISPEPVPRAAVAAAVSR 325
           RRITT I +P+ V RA   AAVSR
Sbjct: 303 RRITTGISTPDAVSRA--VAAVSR 324

BLAST of Spg031794 vs. ExPASy TrEMBL
Match: A0A0A0L524 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G150100 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 7.1e-152
Identity = 279/324 (86.11%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           MALSL+SAAGKQVQ LVGNFQG+FNMEQFFRRKDKVVVVMGATGTGKSRLAI+LATRFP+
Sbjct: 3   MALSLTSAAGKQVQPLVGNFQGNFNMEQFFRRKDKVVVVMGATGTGKSRLAIELATRFPS 62

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVNSDKIQV+EGLDVVTNKVT+EERRGIPHHLL SIDP SNFSS DF HHAS AIESIL
Sbjct: 63  EIVNSDKIQVYEGLDVVTNKVTEEERRGIPHHLLSSIDPKSNFSSRDFTHHASGAIESIL 122

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPIIAGGSNSYIEALV+DYAE+RFRYEFCFLWVDVSLPILQ FVSDRVDRMVDGGF
Sbjct: 123 ARDRLPIIAGGSNSYIEALVNDYAEARFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGF 182

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           V EVR+IFDP+G+YSQGIKRAIGVPELH++LRAERDGADER L ILLELAIS+IKDNTCR
Sbjct: 183 VEEVRQIFDPEGDYSQGIKRAIGVPELHEFLRAERDGADERVLNILLELAISRIKDNTCR 242

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LA RQL+KIRLLR+KWNWNLRRLDATG  LTDG NS  VWEK V+EPSS IVDQFLCD S
Sbjct: 243 LAFRQLEKIRLLRSKWNWNLRRLDATGVILTDGENSLDVWEKLVLEPSSRIVDQFLCDGS 302

Query: 301 RRITTAIISPEPVPRAAVAAAVSR 325
           RRITT I +P+ V RA   AAVSR
Sbjct: 303 RRITTGISTPDAVSRA--VAAVSR 324

BLAST of Spg031794 vs. ExPASy TrEMBL
Match: A0A6J1GV51 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111457751 PE=4 SV=1)

HSP 1 Score: 543.9 bits (1400), Expect = 4.6e-151
Identity = 276/324 (85.19%), Postives = 298/324 (91.98%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           MALSLSSA GKQVQ LVGNFQGSF+MEQFFRRK+KVV+VMGATGTGKSRLAIDLATRFPA
Sbjct: 3   MALSLSSAVGKQVQPLVGNFQGSFSMEQFFRRKEKVVIVMGATGTGKSRLAIDLATRFPA 62

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVNSDKIQ +EGLDVVTNKVTDEERRG+ HHLLGSIDP+SNFSS DF +HASVAIESIL
Sbjct: 63  EIVNSDKIQAYEGLDVVTNKVTDEERRGVSHHLLGSIDPNSNFSSHDFTYHASVAIESIL 122

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPI+AGGSNSYIEALVDD+AESRFRYEFCFLWVDVSLPILQ FVSDRVDRMVDGGF
Sbjct: 123 ARDRLPIVAGGSNSYIEALVDDWAESRFRYEFCFLWVDVSLPILQKFVSDRVDRMVDGGF 182

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           VGEVRE FDP+G+YS+GIKRAIGVPELHDYLRAERDGAD+RTLTILLELAISKIKDNTC+
Sbjct: 183 VGEVREFFDPEGDYSRGIKRAIGVPELHDYLRAERDGADQRTLTILLELAISKIKDNTCQ 242

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LALRQLQKIR LRNK NWNLRRLDATGAFLT+ GN +  W+K V++PS  IVD+FL DDS
Sbjct: 243 LALRQLQKIRRLRNKRNWNLRRLDATGAFLTNSGNPNDAWDKFVLQPSLRIVDKFLTDDS 302

Query: 301 RRITTAIISPEPVPRAAVAAAVSR 325
           R ITT I+SPE  P   + AAVSR
Sbjct: 303 RLITTPIVSPEAAP---LVAAVSR 323

BLAST of Spg031794 vs. ExPASy TrEMBL
Match: A0A6J1F8Q6 (adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111443341 PE=4 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.0e-150
Identity = 274/320 (85.62%), Postives = 294/320 (91.88%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPA 60
           ++LS ++AAGK+V  LVGNFQGSFNMEQFFRRKDKVV VMGATGTGKSRLAIDLATRFPA
Sbjct: 5   LSLSSAAAAGKRVAPLVGNFQGSFNMEQFFRRKDKVVFVMGATGTGKSRLAIDLATRFPA 64

Query: 61  EIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDSNFSSDDFNHHASVAIESIL 120
           EIVN+DKIQV+EGLDVVTNKVTDEERRGI HHLLGSIDP SNFSS DF HHAS AIESIL
Sbjct: 65  EIVNADKIQVYEGLDVVTNKVTDEERRGITHHLLGSIDPYSNFSSHDFTHHASDAIESIL 124

Query: 121 ARDRLPIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGF 180
           ARDRLPIIAGGSNSYIEALVDDY+E RFRY+FCFLWVDVSLPIL NFVSDRVD+MVDGGF
Sbjct: 125 ARDRLPIIAGGSNSYIEALVDDYSEFRFRYDFCFLWVDVSLPILHNFVSDRVDQMVDGGF 184

Query: 181 VGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCR 240
           VGEVREIFDPKG+YS GIKRAIGVPELHDYL+AERDGADERTL+ILLELAISKIKDNTC 
Sbjct: 185 VGEVREIFDPKGDYSYGIKRAIGVPELHDYLKAERDGADERTLSILLELAISKIKDNTCL 244

Query: 241 LALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDDS 300
           LALRQ QKIR L+NK NWNL+RLDAT AFLT+GGNS  VWEKNV+EPSSMIVD+FL DD+
Sbjct: 245 LALRQWQKIRRLQNKRNWNLQRLDATCAFLTEGGNSHPVWEKNVIEPSSMIVDKFLHDDT 304

Query: 301 RRITTAIISPEPVPRAAVAA 321
           R ITT I SPEPV RAA  +
Sbjct: 305 RHITTTIFSPEPVHRAAAVS 324

BLAST of Spg031794 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 352.4 bits (903), Expect = 3.8e-97
Identity = 190/325 (58.46%), Postives = 232/325 (71.38%), Query Frame = 0

Query: 7   SAAGKQVQQLVGNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPAEIVNSD 66
           +A  + +Q L  NFQG+     FFRRKDKVV VMGATGTGKSRLAIDLATRFPAEIVNSD
Sbjct: 6   TALRQVIQPLSLNFQGNMVDVPFFRRKDKVVFVMGATGTGKSRLAIDLATRFPAEIVNSD 65

Query: 67  KIQVFEGLDVVTNKVTDEERRGIPHHLLGSI-DPDSNFSSDDFNHHASVAIESILARDRL 126
           KIQV++GLD+VTNKVT EE  G+PHHLLG++ D   +F+++DF   A  A+ESI+ RDR+
Sbjct: 66  KIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRDRV 125

Query: 127 PIIAGGSNSYIEALVDDYAESRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGFVGEVR 186
           PIIAGGSNSYIEALV+D  + R RY  CFLWVDVS P+L +FVS+RVD+MVD G V EVR
Sbjct: 126 PIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDEVR 185

Query: 187 EIFDP-KGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKDNTCRLALR 246
            IFDP   +YS GI+RAIGVPEL ++LR+E       T   LLE AI KIK+NTC LA R
Sbjct: 186 RIFDPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLACR 245

Query: 247 QLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFL--CDDSRR 306
           QLQKI+ L  +W WN+ R+DAT  FL  G  +D  W+ +V  PS++ V++FL   DD   
Sbjct: 246 QLQKIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFLSYSDDHHL 305

Query: 307 ITTAIISPE---PVPRAAVAAAVSR 325
               I+ PE     P  A  AA+SR
Sbjct: 306 EGANILLPEISAVPPLPAAVAAISR 330

BLAST of Spg031794 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 293.1 bits (749), Expect = 2.7e-79
Identity = 160/300 (53.33%), Postives = 209/300 (69.67%), Query Frame = 0

Query: 1   MALSLSSAAGKQVQQLV--GNFQGSFNMEQFFRRKDKVVVVMGATGTGKSRLAIDLATRF 60
           M  S+SS   KQVQ ++   N     N+  F   K+KV+ VMGATG+GKSRLAIDLATRF
Sbjct: 1   MKFSISSL--KQVQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATRF 60

Query: 61  PAEIVNSDKIQVFEGLDVVTNKVTDEERRGIPHHLLGSIDPDS-NFSSDDFNHHASVAIE 120
             EI+NSDKIQ+++GLDV+TNKVT +E RG+PHHLLG  D ++ N ++  ++  AS AI 
Sbjct: 61  QGEIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAIS 120

Query: 121 SILARDRLPIIAGGSNSYIEALVDDYAESRF-RYEFCFLWVDVSLPILQNFVSDRVDRMV 180
            + A ++LPI+AGGSNSYIEALV+  +      Y+ CF+WVDVSLP+L +FVS RVDRM+
Sbjct: 121 KLSANNKLPIVAGGSNSYIEALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMM 180

Query: 181 DGGFVGEVREIFDPKGNYSQGIKRAIGVPELHDYLRAERDGADERTLTILLELAISKIKD 240
           + G + EVRE+F+PK NYS GI+RAIGVPELH+YLR E    D  T + +L++A+  IK 
Sbjct: 181 EAGLLEEVREVFNPKANYSVGIRRAIGVPELHEYLRNE-SLVDRATKSKMLDVAVKNIKK 240

Query: 241 NTCRLALRQLQKIRLLRNKWNWNLRRLDATGAFL-TDGGNSDAVWEKNVVEPSSMIVDQF 296
           NT  LA RQL+KI+ L  KW  ++ R+DAT  FL  +    D  WE  V  PS  IVD+F
Sbjct: 241 NTEILACRQLKKIQRLHKKWKMSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKF 297

BLAST of Spg031794 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 277.7 bits (709), Expect = 1.2e-74
Identity = 156/299 (52.17%), Postives = 204/299 (68.23%), Query Frame = 0

Query: 33  KDKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVFEGLDVVTNKVTDEERRGIPHH 92
           KDKVVV+MGATGTGKSRL++D+ATRF AEI+NSDKIQV +GLD+VTNK+T EE  G+PHH
Sbjct: 40  KDKVVVIMGATGTGKSRLSVDIATRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHH 99

Query: 93  LLGSIDPDSNFSSDDFNHHASVAIESILARDRLPIIAGGSNSYIEALVDDYAES-RFRYE 152
           LLG + P+++ ++ ++ H A+++IES+L R +LPII GGSNSY+EALVDD     R RY+
Sbjct: 100 LLGVLPPEADLTAANYCHMANLSIESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYD 159

Query: 153 FCFLWVDVSLPILQNFVSDRVDRMVDGGFVGEVREIFD-PKGNYSQGIKRAIGVPELHDY 212
            CFLWVDV+LP+L  FVS+RVD+MV+ G V EVRE FD    +YS+GIK+AIG PE   +
Sbjct: 160 CCFLWVDVALPVLHGFVSERVDKMVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRF 219

Query: 213 LRAERD-GADERTLTILLELAISKIKDNTCRLALRQLQKIRLLRNKWNWNLRRLDATGAF 272
            R E+    ++R    LL   + +IK NT  LA RQ +KI  LR    W+++R+DAT  F
Sbjct: 220 FRNEQFLNVEDR--EELLSKVLEEIKRNTFELACRQREKIERLRKVKKWSIQRVDATPVF 279

Query: 273 LTDGGNSDA--VWEKNVVEPSSMIVDQFLCDDSRRITTAIISPEPVPRA--AVAAAVSR 325
                  DA   WE+ V  PS+  V +FL D        I S  P+  A  AVAAA+ R
Sbjct: 280 TKRRSKMDANVAWERLVAGPSTDTVSRFLLD--------IASRRPLVEASTAVAAAMER 328

BLAST of Spg031794 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 221.1 bits (562), Expect = 1.3e-57
Identity = 127/304 (41.78%), Postives = 185/304 (60.86%), Query Frame = 0

Query: 23  SFNMEQ--FFRRKDKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVFEGLDVVTNK 82
           S  MEQ     RKDKVVV++GATG GKSRL++DLATRFP+EI+NSDKIQV+EGL++ TN+
Sbjct: 52  SIRMEQSRSRNRKDKVVVILGATGAGKSRLSVDLATRFPSEIINSDKIQVYEGLEITTNQ 111

Query: 83  VTDEERRGIPHHLLGSIDPD-SNFSSDDFNHHASVAIESILARDRLPIIAGGSNSYIEAL 142
           +T ++RRG+PHHLLG I+P+    ++ +F   AS  ++ I +R ++PIIAGGSNS++ AL
Sbjct: 112 ITLQDRRGVPHHLLGVINPEHGELTAGEFRSAASNVVKEITSRQKVPIIAGGSNSFVHAL 171

Query: 143 VDDYAESRF--------------RYEFCFLWVDVSLPILQNFVSDRVDRMVDGGFVGEVR 202
           +    + +F              RYE CF+WVDVS  +L  ++  RVD M+D G   E+ 
Sbjct: 172 LAQRFDPKFDPFSSGSCLISSDLRYECCFIWVDVSETVLYEYLLRRVDEMMDSGMFEELS 231

Query: 203 EIFDPKGNYSQ---GIKRAIGVPELHDYLR----AERDGADERTLTILLELAISKIKDNT 262
             +DP  +  +   GI++AIGVPE   Y +     ++    +       + A+  IK NT
Sbjct: 232 RFYDPVKSGLETRFGIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNT 291

Query: 263 CRLALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAV------WEKNVVEPSSMIV 297
             LA RQ++KI +L++   W + R+DAT +F      S +       WE+ V+EPS  IV
Sbjct: 292 WTLAKRQVKKIEMLKDA-GWEIERVDATASFKAVMMKSSSEKKWRENWEEQVLEPSVKIV 351

BLAST of Spg031794 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 219.9 bits (559), Expect = 2.9e-57
Identity = 129/298 (43.29%), Postives = 185/298 (62.08%), Query Frame = 0

Query: 26  MEQFFRRKDKVVVVMGATGTGKSRLAIDLATRFPAEIVNSDKIQVFEGLDVVTNKVTDEE 85
           MEQ +  K KVVV+MGATG+GKS L+IDLATRF  EIVNSDKIQ ++GL V TN+++  E
Sbjct: 37  MEQSY--KQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILE 96

Query: 86  RRGIPHHLLGSIDP-DSNFSSDDFNHHASVAIESILARDRLPIIAGGSNSYIEALVDDYA 145
           R G+PHHLLG + P DS  ++ +F   AS +I  I AR  LPIIAGGSNS+I AL+ D  
Sbjct: 97  RCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRF 156

Query: 146 E-------------SRFRYEFCFLWVDVSLPILQNFVSDRVDRMVDGGFVGEVREIFDPK 205
           +             S  RYE CFLWVDVS+ +L  ++S RVD+M++ G   E+   +DP+
Sbjct: 157 DPKTYPFSSETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDPR 216

Query: 206 GNYS----QGIKRAIGVPELHDYL------RAERDGADERTLTILLELAISKIKDNTCRL 265
            + S     GI + IG+PE   Y       R ++    ++      + A+ +IK+NT RL
Sbjct: 217 YSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRL 276

Query: 266 ALRQLQKIRLLRNKWNWNLRRLDATGAFLTDGGNSDAVWEKNVVEPSSMIVDQFLCDD 300
           A +Q+++I  L++   W+++RLDAT +F   G +S  +W+  V++ S  +V +FL  D
Sbjct: 277 AKKQIERIMKLKSS-GWDIQRLDATPSF---GRSSREIWDNTVLDESIKVVKRFLVKD 328

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898938.12.5e-15990.12adenylate isopentenyltransferase 5, chloroplastic [Benincasa hispida][more]
KAA0049402.12.7e-15387.04adenylate isopentenyltransferase 5 [Cucumis melo var. makuwa] >TYK17157.1 adenyl... [more]
XP_008438727.12.7e-15387.04PREDICTED: adenylate isopentenyltransferase 5, chloroplastic [Cucumis melo][more]
XP_011650995.11.5e-15186.11adenylate isopentenyltransferase 5, chloroplastic [Cucumis sativus] >KGN57030.1 ... [more]
KAG7028930.13.2e-15186.11Adenylate isopentenyltransferase 5, chloroplastic, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
Q94ID25.3e-9658.46Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q94ID13.8e-7853.33Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370... [more]
Q93WC91.7e-7352.17Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Q5GHF73.4e-5842.19Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1[more]
Q94ID31.8e-5641.78Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
A0A5A7U0V61.3e-15387.04Adenylate isopentenyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AXR71.3e-15387.04adenylate isopentenyltransferase 5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A0A0L5247.1e-15286.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G150100 PE=3 SV=1[more]
A0A6J1GV514.6e-15185.19adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=... [more]
A0A6J1F8Q63.0e-15085.63adenylate isopentenyltransferase 5, chloroplastic-like OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
AT5G19040.13.8e-9758.46isopentenyltransferase 5 [more]
AT3G23630.12.7e-7953.33isopentenyltransferase 7 [more]
AT3G63110.11.2e-7452.17isopentenyltransferase 3 [more]
AT1G68460.11.3e-5741.78isopentenyltransferase 1 [more]
AT3G19160.12.9e-5743.29ATP/ADP isopentenyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 33..296
e-value: 5.0E-105
score: 352.5
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 34..190
NoneNo IPR availablePFAMPF01715IPPTcoord: 147..250
e-value: 2.4E-11
score: 43.8
coord: 69..143
e-value: 5.7E-22
score: 78.6
NoneNo IPR availableGENE3D1.10.287.890Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domaincoord: 161..260
e-value: 5.0E-105
score: 352.5
NoneNo IPR availablePANTHERPTHR11088:SF74ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTICcoord: 24..302
IPR039657DimethylallyltransferasePANTHERPTHR11088TRNA DIMETHYLALLYLTRANSFERASEcoord: 24..302

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031794.1Spg031794.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0006400 tRNA modification
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0052381 tRNA dimethylallyltransferase activity