Spg031782 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031782
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionATG8-interacting protein 1
Locationscaffold11: 43361104 .. 43364461 (-)
RNA-Seq ExpressionSpg031782
SyntenySpg031782
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCGAATATCAAAACTGCGACAAACTCAACCTGCTGTAGCGGTTACCCAATCAGTTTTGAAAATCAATTGTCACCGGTAACCGCCGGTTTCCATCATTGCTTGGCGGAGATTATTACTTTACCCCACACATTCACGTCCCAGTCCCAATCCCTTCTCCATTTCCCGAGGCAAATTTCATCCTTATCGTCACGTCGTCGCCGTTCGCCGCAAGTCTCCGATCGCCGCTCTCTCGTCCGGTTCGTCTCTTCTGCTACCGTTCTCTGCAACTTTTGATTGATTCTTTTCTGTTTTTCTCCTTCGATCGTGAAGTTGTGTGGTTGATTGTGATTTTTGGCTTGTTTGATGGACGATCCGATCTTTCTAGTGGTTTTGATCGGTGGTGATCTTTTTTGCCTATTGGTACGGTGTTAGATCCGTCTGTTGTTCGATCTTGAATTCTGGAATGGATGATTATACGACCTAGATATGTGAATTGTGTGTGCGTATGTGCAATTGCGATTAGATTGGTAGTTTATACTTTTGCAGGTCTAATTTCTCTATATTAGATTGGATTTTGTATTCTCTTTATCTTAGAGTGGGAATCGGGCGAGTTTACAACGCGTCGGATTGATGTGGCACGAGGAAATTGTTTTCTTGTTTTCGTTTCCGTGTATGGAGTTGTGTTTTTGGTTGACTTATGGCTTAGTTCTAAAATTTTCCGGAAGAAAGATTGTCATATTGTTGAAATTGCATCTGGAGTTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTCCTTCTTCTTTTTAATCAATCCGTTAAGCTATGTTTTCTTTGCCAGATTCTGCGGGGTTCCAATACACGAGGACTCCCTTAGGATGGCAGATAATGATGGAGAAGAGAATACCTCTCGTGGAAATGAATGGGAGGTTGTTTCACTCACTGCTTCGGCGTATGAAGCTGCTCCTAGTGTGAAGGAAGGTGAATCGACTGATGAAAATGAAAGCAATTTGTACGAAGCAGAAACTTCCCGTGCTCTTTTTATGTCTAGACACTTTGTGTTTCCACCAAGTCAGCACGAGAATTTGCCATTGGAACCTGATAAGAGTGAGATTCACGATGATCAAGGAGTGAAGGAGAATGTGGCGTCTGATTCTGCTGCTGTAGATGGCGGGAAATCTAGTGGAAAGACTGAAGATAGCTTGAATTTAGAAGGTTTGGGTGAAACAGATGAGTTTTCAGGGATTGGTAAAACTATGGAGAAAAGCAGCAAGTTGTCTTTCCATGGTGATGATTTTGGTGAAAATACACCCCTACAAGATTTGAGTCTGGTTGATAAGGAGCAGGATCCCTTCGGTGCTCCTGCATATAGTTCTTTTCATGGTGAAAGCGATCTCAGTTCCACGACATTTGATGAAATCCCTCCCCTGGAACCTACTGATCAAGTCTCAGCTCCAGAAATCTCAGAAACTCCTAGACTCGATAGTAAAAGCAATAAATCCAGTCTTCCTTGTGGTGCTTGGTGGAAGAGAAGAGCTGCTAGTTTATATTCTCATGCAAAAGAAGCGAATGCATTTTGGTCCATTTTCATTGCAGCAGCTGTTATGGGACTTGTAATACTTGGGCAGCGCTGGCAGCAGGAAAGTTGGCAGACTCTGCAACTGAAGTGGCACATTAGTGTGCATGATCAGGTATGTTGATTATTGTTGCCTCCGTTTGGTAGCATTCTCTTTACGGTTCAGATTCTTCAGCTGCTTCGTTATCATTATACATGCATCATTTTGTTATGTACACTGAATTTTAAGTTTCAGATTTAAGTATCTAGAATAGACTAAGAATAGGAGATTCAAATTCAATCTCAACAAAAGCAATGGTGCTGACTACTAAGATCTTATAATAGCAATATTATTTAGGATGATTAATCTTTGTGAGCAGGCTAGCTCATTATTTCTTTGAAGATGCATATGTTGCTTTGACTTGATGTCGAACTACTAGGCTTAAATTTTACAAGTGGAAACTAGAACCAATCTGAATGAGCTCATTGGTGGCAGAAATTCGGGGACCTTCCAACAAGGAAACCAGTTTTCTTGAATACAAACCGATTGAATAGCTAATTCTGATTCTGATCAGGGGAACATGTTATATGGTTATAAAGCCAACTTTTTGTCGTGCCCACTATTTTCCAAGTCTCATCTTCGCAGCTGTTGAGGAAATATGCGCGGAGTTTCATGTTAACGTTTTTTTTTTTTAAAATTTAATTTTTGAGTTCAACAACATTTGGGGATGGGGATAAAGAGTTGTCATATACTCTCGACCTGTCTGAGGTTCATATTAAAGTTTTTGGTTTTTAATTTTTGACATTTAAAGAATTTAAAACTGAAAAATATTTGGCTGCAATCCTTACCTACCCCATTAAAATCTTGACAACTAAAAAAGAGAAAGGTAAAAAATGAATTTTTTTTAATCTCTCTTAATTCTCAAGATTAATCCCTACATACTAGTACGGAGCAAAGTATTTTTCTTTAAAACTGATACATTTATGGGTTTTTTATTTTTTTATTTTTTTAAAAAAACTGCCACATTAGCCATCTGTTGATAGTCATTTTTCAAGTTGTGTCAGTTTAAATGATTGGATTGGTCTGCATATTGGCTTTTTGACATGACTACCACACACAAGCCGTGCCATCAATTACATGTTGATGCTTCCACCCTGCTCTAGTGTTTATAGCAATGCAATAAGTCCTCTGGTTCAATTTTTTAGATATTAGTTTACATTATTTATCGCCATTGCAACTCATGTTCGCACTGTGGTTATATAAATGCTCTCTTTTGACTGTTTTAGAAGAGATGTCTGCTTCCCTTTTCGCGTTGCTATCTTCTGGCCTTAATTTTCATTATACTGCTACAATTGCAGAAGACAAACCGAGTGCTGGGACCGATAACTCGGCTTAAAGATGTGATCGTAGGTGGCCAACGCCGCAGTTCGTCTGTCAAGTTCAGCTCCAGTGAGTTATAAGATGATGACGATAAGTGTAGAAAAGGTCGAAAGGAAGAAACCGAATGAAAGAGAGGTTTGTTAGTTGAATGAATACCTGAGTGTCTTTGGCCGTCTTGCAAAATTTACACAATTACTTTGGGGAACATGTTGGAGTTTGATTGTTTAAATTATATCTTATCTTGGTGGTATAGTACCATATTGCATTGTACATTATCTTAATCAATTGGATGAAATGTAAGTTTTGAGGTCGTTGTCCCTATCCCCGTGGGGTTTATTTGGGCTCATTTTATTTCAACTTTGTCGAGAACTACCCTGCCAATTAGTTTCAATGACACATTATAGATCACTGCATAAGATTATCTTGTTCATCTAA

mRNA sequence

ATGCCTCGAATATCAAAACTGCGACAAACTCAACCTGCTGTAGCGGTTACCCAATCAGTTTTGAAAATCAATTGTCACCGTCCCAATCCCTTCTCCATTTCCCGAGGCAAATTTCATCCTTATCGTCACGTCGTCGCCGTTCGCCGCAAGTCTCCGATCGCCGCTCTCTCGTCCGAGTGGGAATCGGGCGAGTTTACAACGCGTCGGATTGATGTGGCACGAGGAAATTGTTTTCTTGTTTTCGTTTCCGTGTATGGAGTTGTGTTTTTGGTTGACTTATGGCTTAGTTCTAAAATTTTCCGGAAGAAAGATTGTCATATTGTTGAAATTGCATCTGGAGTTGTATTCTGCGGGGTTCCAATACACGAGGACTCCCTTAGGATGGCAGATAATGATGGAGAAGAGAATACCTCTCGTGGAAATGAATGGGAGGTTGTTTCACTCACTGCTTCGGCGTATGAAGCTGCTCCTAGTGTGAAGGAAGGTGAATCGACTGATGAAAATGAAAGCAATTTGTACGAAGCAGAAACTTCCCGTGCTCTTTTTATGTCTAGACACTTTGTGTTTCCACCAAGTCAGCACGAGAATTTGCCATTGGAACCTGATAAGAGTGAGATTCACGATGATCAAGGAGTGAAGGAGAATGTGGCGTCTGATTCTGCTGCTGTAGATGGCGGGAAATCTAGTGGAAAGACTGAAGATAGCTTGAATTTAGAAGGTTTGGGTGAAACAGATGAGTTTTCAGGGATTGGTAAAACTATGGAGAAAAGCAGCAAGTTGTCTTTCCATGGTGATGATTTTGGTGAAAATACACCCCTACAAGATTTGAGTCTGGTTGATAAGGAGCAGGATCCCTTCGGTGCTCCTGCATATAGTTCTTTTCATGGTGAAAGCGATCTCAGTTCCACGACATTTGATGAAATCCCTCCCCTGGAACCTACTGATCAAGTCTCAGCTCCAGAAATCTCAGAAACTCCTAGACTCGATAGTAAAAGCAATAAATCCAGTCTTCCTTGTGGTGCTTGGTGGAAGAGAAGAGCTGCTAGTTTATATTCTCATGCAAAAGAAGCGAATGCATTTTGGTCCATTTTCATTGCAGCAGCTGTTATGGGACTTGTAATACTTGGGCAGCGCTGGCAGCAGGAAAGTTGGCAGACTCTGCAACTGAAGTGGCACATTAGTGTGCATGATCAGAAGACAAACCGAGTGCTGGGACCGATAACTCGGCTTAAAGATGTGATCGTAGGTGGCCAACGCCGCAGTTCGTCTGTCAAGTTCAGCTCCAGTGAGTTATAA

Coding sequence (CDS)

ATGCCTCGAATATCAAAACTGCGACAAACTCAACCTGCTGTAGCGGTTACCCAATCAGTTTTGAAAATCAATTGTCACCGTCCCAATCCCTTCTCCATTTCCCGAGGCAAATTTCATCCTTATCGTCACGTCGTCGCCGTTCGCCGCAAGTCTCCGATCGCCGCTCTCTCGTCCGAGTGGGAATCGGGCGAGTTTACAACGCGTCGGATTGATGTGGCACGAGGAAATTGTTTTCTTGTTTTCGTTTCCGTGTATGGAGTTGTGTTTTTGGTTGACTTATGGCTTAGTTCTAAAATTTTCCGGAAGAAAGATTGTCATATTGTTGAAATTGCATCTGGAGTTGTATTCTGCGGGGTTCCAATACACGAGGACTCCCTTAGGATGGCAGATAATGATGGAGAAGAGAATACCTCTCGTGGAAATGAATGGGAGGTTGTTTCACTCACTGCTTCGGCGTATGAAGCTGCTCCTAGTGTGAAGGAAGGTGAATCGACTGATGAAAATGAAAGCAATTTGTACGAAGCAGAAACTTCCCGTGCTCTTTTTATGTCTAGACACTTTGTGTTTCCACCAAGTCAGCACGAGAATTTGCCATTGGAACCTGATAAGAGTGAGATTCACGATGATCAAGGAGTGAAGGAGAATGTGGCGTCTGATTCTGCTGCTGTAGATGGCGGGAAATCTAGTGGAAAGACTGAAGATAGCTTGAATTTAGAAGGTTTGGGTGAAACAGATGAGTTTTCAGGGATTGGTAAAACTATGGAGAAAAGCAGCAAGTTGTCTTTCCATGGTGATGATTTTGGTGAAAATACACCCCTACAAGATTTGAGTCTGGTTGATAAGGAGCAGGATCCCTTCGGTGCTCCTGCATATAGTTCTTTTCATGGTGAAAGCGATCTCAGTTCCACGACATTTGATGAAATCCCTCCCCTGGAACCTACTGATCAAGTCTCAGCTCCAGAAATCTCAGAAACTCCTAGACTCGATAGTAAAAGCAATAAATCCAGTCTTCCTTGTGGTGCTTGGTGGAAGAGAAGAGCTGCTAGTTTATATTCTCATGCAAAAGAAGCGAATGCATTTTGGTCCATTTTCATTGCAGCAGCTGTTATGGGACTTGTAATACTTGGGCAGCGCTGGCAGCAGGAAAGTTGGCAGACTCTGCAACTGAAGTGGCACATTAGTGTGCATGATCAGAAGACAAACCGAGTGCTGGGACCGATAACTCGGCTTAAAGATGTGATCGTAGGTGGCCAACGCCGCAGTTCGTCTGTCAAGTTCAGCTCCAGTGAGTTATAA

Protein sequence

MPRISKLRQTQPAVAVTQSVLKINCHRPNPFSISRGKFHPYRHVVAVRRKSPIAALSSEWESGEFTTRRIDVARGNCFLVFVSVYGVVFLVDLWLSSKIFRKKDCHIVEIASGVVFCGVPIHEDSLRMADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTDENESNLYEAETSRALFMSRHFVFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDEFSGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDEIPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFSSSEL
Homology
BLAST of Spg031782 vs. NCBI nr
Match: KAG7028597.1 (ATG8-interacting protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 553.5 bits (1425), Expect = 1.6e-153
Identity = 285/337 (84.57%), Postives = 301/337 (89.32%), Query Frame = 0

Query: 95  LSSKIFRKKDCHIVEIASGVVFCGVPIHEDSLRMADNDGEENTSRGNEWEVVSLTASAYE 154
           LSS+  R++   I +  S V F  VP HEDSLRMADNDGE++TSRG EWEVVSLTASAYE
Sbjct: 27  LSSR--RRRSSRISDRLSLVRFWEVPAHEDSLRMADNDGEDSTSRGTEWEVVSLTASAYE 86

Query: 155 AAPSVKEGESTDENESNLYEAETSRALFMSRHFVFPPSQHENLPLEPDKSEIHDDQGVKE 214
           AAPS KE E +DEN+SNLYEAETS ALFMS+HFVFPPSQHENLPLEPDKSEIHDDQGVKE
Sbjct: 87  AAPSAKEDEQSDENKSNLYEAETSHALFMSKHFVFPPSQHENLPLEPDKSEIHDDQGVKE 146

Query: 215 NVASDSAAVDGGKSSGKTEDSLNLEGLGETDEFSGIGKTMEKSSKLSFHGDDFGENTPLQ 274
           NV SDSAAVDGGKS GK+EDSLNLEGLGETDEFSGI KT EK SKLSFHGDDFGENT LQ
Sbjct: 147 NVVSDSAAVDGGKSGGKSEDSLNLEGLGETDEFSGIDKTTEKGSKLSFHGDDFGENTTLQ 206

Query: 275 DLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDEIPPLEPTDQVSAPEISETPRLDSKSNK 334
           DL+LV KEQD FGAP YSSFH ESDLSSTTFDEIPP EPTDQVS PEISETPRL+SKS K
Sbjct: 207 DLNLVHKEQDLFGAPTYSSFHAESDLSSTTFDEIPPPEPTDQVSTPEISETPRLNSKSKK 266

Query: 335 SSLPCGAWWKRRAASLYSHAKEANAFWSIFIAAAVMGLVILGQRWQQESWQTLQLKWHIS 394
           SSLPCGAWWKRRAASLYSHAKEANAFWSIFIAAAVMGLVILGQRWQQESWQ+LQLKWHIS
Sbjct: 267 SSLPCGAWWKRRAASLYSHAKEANAFWSIFIAAAVMGLVILGQRWQQESWQSLQLKWHIS 326

Query: 395 VHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFSSSEL 432
           V+DQKTNRVLGPI RLKDVIVG QRR SS+K S +EL
Sbjct: 327 VNDQKTNRVLGPIARLKDVIVGAQRRGSSIKSSPNEL 361

BLAST of Spg031782 vs. NCBI nr
Match: KAG6597130.1 (ATG8-interacting protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 551.6 bits (1420), Expect = 6.0e-153
Identity = 284/337 (84.27%), Postives = 300/337 (89.02%), Query Frame = 0

Query: 95  LSSKIFRKKDCHIVEIASGVVFCGVPIHEDSLRMADNDGEENTSRGNEWEVVSLTASAYE 154
           LSS+  R++   I +  S V F  VP HEDSLRMADNDGE++TSRG EWEVVSLTASAYE
Sbjct: 21  LSSR--RRRSSRISDRLSLVRFWEVPAHEDSLRMADNDGEDSTSRGTEWEVVSLTASAYE 80

Query: 155 AAPSVKEGESTDENESNLYEAETSRALFMSRHFVFPPSQHENLPLEPDKSEIHDDQGVKE 214
           AAPS KE E +DEN+SNLYEAETS ALFMS+HFVFPPSQHENLPLEPDKSEIHDDQGVKE
Sbjct: 81  AAPSAKEDEQSDENKSNLYEAETSHALFMSKHFVFPPSQHENLPLEPDKSEIHDDQGVKE 140

Query: 215 NVASDSAAVDGGKSSGKTEDSLNLEGLGETDEFSGIGKTMEKSSKLSFHGDDFGENTPLQ 274
           NV SDSAAVDGGKS GK+EDSLNLEGLGETDEFSGI KT EK SKLSFHGDDFGENT LQ
Sbjct: 141 NVVSDSAAVDGGKSGGKSEDSLNLEGLGETDEFSGIDKTTEKGSKLSFHGDDFGENTTLQ 200

Query: 275 DLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDEIPPLEPTDQVSAPEISETPRLDSKSNK 334
           DL+LV KEQD FGAP YSSFH ESDLSSTTFDEIPP EPTDQVS PEISETPRL+ KS K
Sbjct: 201 DLNLVHKEQDLFGAPTYSSFHAESDLSSTTFDEIPPPEPTDQVSTPEISETPRLNRKSKK 260

Query: 335 SSLPCGAWWKRRAASLYSHAKEANAFWSIFIAAAVMGLVILGQRWQQESWQTLQLKWHIS 394
           SSLPCGAWWKRRAASLYSHAKEANAFWSIFIAAAVMGLVILGQRWQQESWQ+LQLKWHIS
Sbjct: 261 SSLPCGAWWKRRAASLYSHAKEANAFWSIFIAAAVMGLVILGQRWQQESWQSLQLKWHIS 320

Query: 395 VHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFSSSEL 432
           V+DQKTNRVLGPI RLKDVIVG QRR SS+K S +EL
Sbjct: 321 VNDQKTNRVLGPIARLKDVIVGAQRRGSSIKSSPNEL 355

BLAST of Spg031782 vs. NCBI nr
Match: KAE8650248.1 (hypothetical protein Csa_010335 [Cucumis sativus])

HSP 1 Score: 539.7 bits (1389), Expect = 2.4e-149
Identity = 276/325 (84.92%), Postives = 290/325 (89.23%), Query Frame = 0

Query: 107 IVEIASGVVFCGVPIHEDSLRMADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTD 166
           I  +A    F  V IH+DS RMAD DGEEN SRGNEWEVVSLTASAYEAAP+VKE ES D
Sbjct: 266 IFVVAYWYEFGEVGIHKDSPRMADKDGEENNSRGNEWEVVSLTASAYEAAPNVKEDESLD 325

Query: 167 ENESNLYEAETSRALFMSRHFVFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGG 226
           EN SNLYEAETSRALFMSRHFVFPPSQHENLPLEPDKSEIHDDQG KENV SDSA +DGG
Sbjct: 326 ENNSNLYEAETSRALFMSRHFVFPPSQHENLPLEPDKSEIHDDQGGKENVESDSAVIDGG 385

Query: 227 KSSGKTEDSLNLEGLGETDEFSGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPF 286
           KSS K EDSLNLEGL ETDEF+GIGKTMEK SKLSFHGDDF ENT L DL+LVDKE DPF
Sbjct: 386 KSSRKNEDSLNLEGLVETDEFTGIGKTMEKGSKLSFHGDDFSENTALPDLNLVDKELDPF 445

Query: 287 GAPAYSSFHGESDLSSTTFDEIPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRR 346
            AP YSSFHGE+DLSSTTFDEIPPLE  DQVS+PE  ETP+LD+KSNKSSLPCGAWWKRR
Sbjct: 446 SAPTYSSFHGETDLSSTTFDEIPPLESNDQVSSPESLETPKLDNKSNKSSLPCGAWWKRR 505

Query: 347 AASLYSHAKEANAFWSIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGP 406
           AA+LYSHAKEA AFWSIFIAAAVMGLVILGQRWQQESWQTLQLKWHISV+DQKTNRVLGP
Sbjct: 506 AANLYSHAKEAKAFWSIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVNDQKTNRVLGP 565

Query: 407 ITRLKDVIVGGQRRSSSVKFSSSEL 432
           ITRLKDVIVGGQRR SS+KFS +EL
Sbjct: 566 ITRLKDVIVGGQRRGSSIKFSPNEL 590

BLAST of Spg031782 vs. NCBI nr
Match: XP_023540318.1 (ATG8-interacting protein 1 [Cucurbita pepo subsp. pepo] >XP_023540319.1 ATG8-interacting protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 535.0 bits (1377), Expect = 5.8e-148
Identity = 271/304 (89.14%), Postives = 283/304 (93.09%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTDENESNLYEAETSRALFMSRHF 187
           MADNDGE++TSRG EWEVVSLTASAYEAAPS KE E +DEN+SNLYEAETS ALFMS+HF
Sbjct: 1   MADNDGEDSTSRGTEWEVVSLTASAYEAAPSAKEDEQSDENKSNLYEAETSHALFMSKHF 60

Query: 188 VFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDEF 247
           VFPPSQHENLPLEPDKSEIHDDQGVKENV SDSAAVDGGKS GK+EDSLNLEGLGETDEF
Sbjct: 61  VFPPSQHENLPLEPDKSEIHDDQGVKENVVSDSAAVDGGKSGGKSEDSLNLEGLGETDEF 120

Query: 248 SGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDE 307
           SGI KTMEK SKLSFHGDDFGENT LQDL+LV KEQD FGAP YSSFH ESDLSSTTFDE
Sbjct: 121 SGIDKTMEKGSKLSFHGDDFGENTTLQDLNLVHKEQDLFGAPTYSSFHAESDLSSTTFDE 180

Query: 308 IPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA 367
           IPP EPTDQVS PEISETPRL+SKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA
Sbjct: 181 IPPPEPTDQVSTPEISETPRLNSKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA 240

Query: 368 AVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFS 427
           AVMGLVILGQRWQQESWQ+LQLKWHISV+DQKTNRVLGPI RLKDVIVG QRR SS+K S
Sbjct: 241 AVMGLVILGQRWQQESWQSLQLKWHISVNDQKTNRVLGPIARLKDVIVGAQRRGSSIKSS 300

Query: 428 SSEL 432
            +EL
Sbjct: 301 PNEL 304

BLAST of Spg031782 vs. NCBI nr
Match: KAG6582617.1 (ATG8-interacting protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 534.3 bits (1375), Expect = 1.0e-147
Identity = 292/408 (71.57%), Postives = 310/408 (75.98%), Query Frame = 0

Query: 24  NCHRPNPFSISRGKFHPYRHVVAVRRKSPIAALSSEWESGEFTTRRIDVARGNCFLVFVS 83
           +C  PNPFSISR KFHPYR+V AV              SG             C      
Sbjct: 40  SCPSPNPFSISRAKFHPYRYVDAV--------------SGR-----------RC------ 99

Query: 84  VYGVVFLVDLWLSSKIFRKKDCHIVEIASGVVFCGVPIHEDSLRMADNDGEENTSRGNEW 143
                      LSS + R              FC V IHE+SLRMADNDGEEN SRGN W
Sbjct: 100 -----------LSSSLAR--------------FCEVGIHENSLRMADNDGEENNSRGNGW 159

Query: 144 EVVSLTASAYEAAPSVKEGESTDENESNLYEAETSRALFMSRHFVFPPSQHENLPLEPDK 203
           EVVSLTASAYEAAP+VKE ESTDEN+SNLYEAETS ALFMSRHFVFPP+QHENLPLEPDK
Sbjct: 160 EVVSLTASAYEAAPNVKESESTDENKSNLYEAETSHALFMSRHFVFPPNQHENLPLEPDK 219

Query: 204 SEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDEFSGIGKTMEKSSKLSFH 263
           SEIHDDQG KENV SDS AVDGGKSS K ED LNLE LGETDEFSGI KT EK  KLSFH
Sbjct: 220 SEIHDDQGGKENVDSDSTAVDGGKSSRKNEDVLNLERLGETDEFSGIDKTTEKGGKLSFH 279

Query: 264 GDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDEIPPLEPTDQVSAPEIS 323
           GD+F ENT L +L+LVDKEQDPF AP YSSFHGE++LSS T DEIPPLE  DQVS PE  
Sbjct: 280 GDEFSENTTLPELNLVDKEQDPFDAPTYSSFHGETNLSSMTNDEIPPLESNDQVSNPESL 339

Query: 324 ETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAAAVMGLVILGQRWQQES 383
           ET RLDSKSNKS+LPCGAWWKRRAA+LYSHAKEANAFWSIF+AAAVMGLVILGQRWQQES
Sbjct: 340 ETRRLDSKSNKSNLPCGAWWKRRAANLYSHAKEANAFWSIFVAAAVMGLVILGQRWQQES 391

Query: 384 WQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFSSSEL 432
           W+TLQLK HISV+DQKT RVLGPITRLKDVIVGGQRR SS+KFS SEL
Sbjct: 400 WRTLQLKGHISVNDQKTIRVLGPITRLKDVIVGGQRRGSSIKFSPSEL 391

BLAST of Spg031782 vs. ExPASy Swiss-Prot
Match: O82775 (ATG8-interacting protein 1 OS=Arabidopsis thaliana OX=3702 GN=ATI1 PE=1 SV=2)

HSP 1 Score: 193.0 bits (489), Expect = 7.1e-48
Identity = 131/312 (41.99%), Postives = 168/312 (53.85%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTDENESN-LYEAETSRALFMSRH 187
           MA+N  EE+  RGNEWEVVSLT+SAY AAP     ES D  + +  Y AETSR L+MS H
Sbjct: 1   MANN--EEHPPRGNEWEVVSLTSSAYAAAPGPYNVESRDVRKYDAYYGAETSRDLYMSEH 60

Query: 188 FVFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDE 247
           FVFPPS+HENLP++                  +S  V   +  G+    L LEG G +D+
Sbjct: 61  FVFPPSEHENLPID------------------ESLFVAEQRKDGR---DLMLEGQGLSDQ 120

Query: 248 F---------SGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGE 307
           F         S  G++   SS+   H + FG  + + +  LVD E +      +S   GE
Sbjct: 121 FHYEAGNNQQSIYGESALGSSR---HMESFGSESAVYEHGLVDAEGN---LDLHSDGEGE 180

Query: 308 SDLSSTTFDEIPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEA 367
            D+  +T                               +LPC AWWKRRA S+YS  +EA
Sbjct: 181 KDVKKST------------------------------HNLPCEAWWKRRAISMYSRTREA 240

Query: 368 NAFWSIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGG 427
           NA WS+F AAAV GLV+LGQRWQQE WQ LQLKW  S+  +K  RVL P++RLKDVIV  
Sbjct: 241 NAIWSLFFAAAVTGLVVLGQRWQQERWQVLQLKWQSSISSEKLGRVLEPLSRLKDVIVRS 253

Query: 428 QRRSSSVKFSSS 430
             ++S V+  SS
Sbjct: 301 NPQASLVRSGSS 253

BLAST of Spg031782 vs. ExPASy Swiss-Prot
Match: Q8VY98 (ATG8-interacting protein 2 OS=Arabidopsis thaliana OX=3702 GN=ATI2 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 5.3e-43
Identity = 127/307 (41.37%), Postives = 161/307 (52.44%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVK---EGESTDENE-SNLYEAETSRALFM 187
           MAD D  E  +RGN+WEVVSLTASAY AAP  K   + +  D  E +  YEAETS  L+M
Sbjct: 1   MADKD--EAATRGNDWEVVSLTASAYAAAPGPKPVVDSKDDDHKEVTPCYEAETSHPLYM 60

Query: 188 SRHFVFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGE 247
           SRHFVFPP+                  G  EN  SD        S  K    L+L+GL  
Sbjct: 61  SRHFVFPPT------------------GQLEN-TSDLTEASLTGSHCKEGSDLSLKGLDL 120

Query: 248 TDEFSGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSST 307
           +D+F G+  + +K  K         EN     +S +D E+   G+  Y            
Sbjct: 121 SDDFGGLEFSEDKGKK--------EENIYTTAMSSLDDERAIGGSHVYE----------- 180

Query: 308 TFDEIPPLEPTDQVSAPEIS--ETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFW 367
                P  EPT+ VS  +++    P  D +   S     AWWKR  ASL + AKE N  W
Sbjct: 181 -----PVEEPTEPVSPSDVTLDLNPIKDDEVANSPPSEEAWWKRSVASLIAQAKETNTVW 240

Query: 368 SIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRS 427
           SI IAAAVMG+VILGQ WQQE WQ LQ KW  S+ ++K  R++GPI+RLK   VGGQRR 
Sbjct: 241 SICIAAAVMGIVILGQHWQQERWQILQQKWESSIGNEKAGRLMGPISRLKQAFVGGQRRD 262

Query: 428 SSVKFSS 429
           S ++ S+
Sbjct: 301 SFIRASA 262

BLAST of Spg031782 vs. ExPASy TrEMBL
Match: A0A0A0L483 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122320 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 4.5e-146
Identity = 267/304 (87.83%), Postives = 279/304 (91.78%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTDENESNLYEAETSRALFMSRHF 187
           MAD DGEEN SRGNEWEVVSLTASAYEAAP+VKE ES DEN SNLYEAETSRALFMSRHF
Sbjct: 1   MADKDGEENNSRGNEWEVVSLTASAYEAAPNVKEDESLDENNSNLYEAETSRALFMSRHF 60

Query: 188 VFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDEF 247
           VFPPSQHENLPLEPDKSEIHDDQG KENV SDSA +DGGKSS K EDSLNLEGL ETDEF
Sbjct: 61  VFPPSQHENLPLEPDKSEIHDDQGGKENVESDSAVIDGGKSSRKNEDSLNLEGLVETDEF 120

Query: 248 SGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDE 307
           +GIGKTMEK SKLSFHGDDF ENT L DL+LVDKE DPF AP YSSFHGE+DLSSTTFDE
Sbjct: 121 TGIGKTMEKGSKLSFHGDDFSENTALPDLNLVDKELDPFSAPTYSSFHGETDLSSTTFDE 180

Query: 308 IPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA 367
           IPPLE  DQVS+PE  ETP+LD+KSNKSSLPCGAWWKRRAA+LYSHAKEA AFWSIFIAA
Sbjct: 181 IPPLESNDQVSSPESLETPKLDNKSNKSSLPCGAWWKRRAANLYSHAKEAKAFWSIFIAA 240

Query: 368 AVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFS 427
           AVMGLVILGQRWQQESWQTLQLKWHISV+DQKTNRVLGPITRLKDVIVGGQRR SS+KFS
Sbjct: 241 AVMGLVILGQRWQQESWQTLQLKWHISVNDQKTNRVLGPITRLKDVIVGGQRRGSSIKFS 300

Query: 428 SSEL 432
            +EL
Sbjct: 301 PNEL 304

BLAST of Spg031782 vs. ExPASy TrEMBL
Match: A0A1S3AVI5 (ATG8-interacting protein 1 OS=Cucumis melo OX=3656 GN=LOC103483267 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 2.5e-144
Identity = 263/304 (86.51%), Postives = 278/304 (91.45%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTDENESNLYEAETSRALFMSRHF 187
           MAD DGEEN SRGNEWEVVSLTASAYEAAP+VKE ES DEN SNLYEAETSRALFMSRHF
Sbjct: 1   MADKDGEENNSRGNEWEVVSLTASAYEAAPNVKEDESPDENNSNLYEAETSRALFMSRHF 60

Query: 188 VFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDEF 247
           VFPPSQHENLPLEPDKSEIHDDQG KENV SDSA +DGGKSS K EDSLNLEGL ETDEF
Sbjct: 61  VFPPSQHENLPLEPDKSEIHDDQGGKENVESDSAVIDGGKSSRKNEDSLNLEGLVETDEF 120

Query: 248 SGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDE 307
           +GIGKTMEK S+LSFHGDDF ENT + DL+LVDKE DPFGAP YSSFHGE+DLSSTTFDE
Sbjct: 121 TGIGKTMEKGSELSFHGDDFSENTTIPDLNLVDKELDPFGAPTYSSFHGETDLSSTTFDE 180

Query: 308 IPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA 367
           IPP E  DQVS+P+  ETPRLD+KSNKSSLPCGAWWKRRAA+LY HAKEA AFWSIFIAA
Sbjct: 181 IPPSESNDQVSSPKSLETPRLDNKSNKSSLPCGAWWKRRAANLYCHAKEAKAFWSIFIAA 240

Query: 368 AVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFS 427
           AVMGLVILGQRWQQESWQTLQLKWHISV+DQKTNRVLGPITRLKDVIVGGQRR SS+KF+
Sbjct: 241 AVMGLVILGQRWQQESWQTLQLKWHISVNDQKTNRVLGPITRLKDVIVGGQRRGSSIKFT 300

Query: 428 SSEL 432
            +EL
Sbjct: 301 PNEL 304

BLAST of Spg031782 vs. ExPASy TrEMBL
Match: A0A5A7TZB9 (ATG8-interacting protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004740 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 2.5e-144
Identity = 263/304 (86.51%), Postives = 278/304 (91.45%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTDENESNLYEAETSRALFMSRHF 187
           MAD DGEEN SRGNEWEVVSLTASAYEAAP+VKE ES DEN SNLYEAETSRALFMSRHF
Sbjct: 1   MADKDGEENNSRGNEWEVVSLTASAYEAAPNVKEDESPDENNSNLYEAETSRALFMSRHF 60

Query: 188 VFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDEF 247
           VFPPSQHENLPLEPDKSEIHDDQG KENV SDSA +DGGKSS K EDSLNLEGL ETDEF
Sbjct: 61  VFPPSQHENLPLEPDKSEIHDDQGGKENVESDSAVIDGGKSSRKNEDSLNLEGLVETDEF 120

Query: 248 SGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDE 307
           +GIGKTMEK S+LSFHGDDF ENT + DL+LVDKE DPFGAP YSSFHGE+DLSSTTFDE
Sbjct: 121 TGIGKTMEKGSELSFHGDDFSENTTIPDLNLVDKELDPFGAPTYSSFHGETDLSSTTFDE 180

Query: 308 IPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA 367
           IPP E  DQVS+P+  ETPRLD+KSNKSSLPCGAWWKRRAA+LY HAKEA AFWSIFIAA
Sbjct: 181 IPPSESNDQVSSPKSLETPRLDNKSNKSSLPCGAWWKRRAANLYCHAKEAKAFWSIFIAA 240

Query: 368 AVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFS 427
           AVMGLVILGQRWQQESWQTLQLKWHISV+DQKTNRVLGPITRLKDVIVGGQRR SS+KF+
Sbjct: 241 AVMGLVILGQRWQQESWQTLQLKWHISVNDQKTNRVLGPITRLKDVIVGGQRRGSSIKFT 300

Query: 428 SSEL 432
            +EL
Sbjct: 301 PNEL 304

BLAST of Spg031782 vs. ExPASy TrEMBL
Match: A0A6J1G3Q9 (ATG8-interacting protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450502 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 3.2e-144
Identity = 264/304 (86.84%), Postives = 279/304 (91.78%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTDENESNLYEAETSRALFMSRHF 187
           MADNDGE++T+RG EWEVVSLTASAYEAAPS KE E +DEN+SNLYEAETS ALFMS+HF
Sbjct: 1   MADNDGEDSTARGTEWEVVSLTASAYEAAPSAKEDEQSDENKSNLYEAETSHALFMSKHF 60

Query: 188 VFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDEF 247
           VFPPSQHENLPLEPDKSEIH+DQGVKENV SDSAAVDGG S GK+EDSLNLEGLGETDEF
Sbjct: 61  VFPPSQHENLPLEPDKSEIHNDQGVKENVVSDSAAVDGGNSGGKSEDSLNLEGLGETDEF 120

Query: 248 SGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDE 307
           SGI K  EK SKLSFHGDDFGENT LQDL+LV KEQD FGAP YSSFH ESDLSSTTFDE
Sbjct: 121 SGIDKITEKGSKLSFHGDDFGENTTLQDLNLVHKEQDLFGAPTYSSFHAESDLSSTTFDE 180

Query: 308 IPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA 367
           IPP EPTDQVS PEISETPRL+SKS KSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA
Sbjct: 181 IPPPEPTDQVSTPEISETPRLNSKSKKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA 240

Query: 368 AVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFS 427
           AVMGLVILGQRW+QESWQ+LQLKWHISV+DQKTNRVLGPI RLKDVIVG QRR SS+K S
Sbjct: 241 AVMGLVILGQRWKQESWQSLQLKWHISVNDQKTNRVLGPIARLKDVIVGAQRRGSSIKSS 300

Query: 428 SSEL 432
            +EL
Sbjct: 301 PNEL 304

BLAST of Spg031782 vs. ExPASy TrEMBL
Match: A0A6J1ITB6 (ATG8-interacting protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479169 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 3.1e-139
Identity = 260/304 (85.53%), Postives = 273/304 (89.80%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTDENESNLYEAETSRALFMSRHF 187
           MADNDGEEN SRGN WEVVSLTASAYEAAP+VKE ESTDEN+SNLYEAETS ALFMSRHF
Sbjct: 1   MADNDGEENNSRGNGWEVVSLTASAYEAAPNVKESESTDENKSNLYEAETSNALFMSRHF 60

Query: 188 VFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDEF 247
           VFPP+QHENLPLEPDKSEIHDD G KENV SDSA+VDGGKSS K ED LNLE LGETDEF
Sbjct: 61  VFPPNQHENLPLEPDKSEIHDDHGGKENVDSDSASVDGGKSSRKNEDVLNLERLGETDEF 120

Query: 248 SGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSSTTFDE 307
           SGI KT EK  KLSFHGD+F ENT L DL+LVDKEQDPF AP YSSFHGE++LSS T DE
Sbjct: 121 SGIDKTTEKGGKLSFHGDEFSENTTLPDLNLVDKEQDPFDAPTYSSFHGETNLSSMTNDE 180

Query: 308 IPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFWSIFIAA 367
           IPPLE  DQVS PE  ET RLDSKSNKS+LPCGAWWKRRAA+LYSHAKEANAFWSIFIAA
Sbjct: 181 IPPLESNDQVSNPESLETHRLDSKSNKSNLPCGAWWKRRAANLYSHAKEANAFWSIFIAA 240

Query: 368 AVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRSSSVKFS 427
           AVMGLVILGQRWQQESW+TLQLK HISV+DQKTNRVLGPITRLKDVIVGGQRR SS+KFS
Sbjct: 241 AVMGLVILGQRWQQESWRTLQLKGHISVNDQKTNRVLGPITRLKDVIVGGQRRGSSIKFS 300

Query: 428 SSEL 432
            SEL
Sbjct: 301 PSEL 304

BLAST of Spg031782 vs. TAIR 10
Match: AT2G45980.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G00355.2); Has 93 Blast hits to 90 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 93; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 193.0 bits (489), Expect = 5.1e-49
Identity = 131/312 (41.99%), Postives = 168/312 (53.85%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVKEGESTDENESN-LYEAETSRALFMSRH 187
           MA+N  EE+  RGNEWEVVSLT+SAY AAP     ES D  + +  Y AETSR L+MS H
Sbjct: 1   MANN--EEHPPRGNEWEVVSLTSSAYAAAPGPYNVESRDVRKYDAYYGAETSRDLYMSEH 60

Query: 188 FVFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGETDE 247
           FVFPPS+HENLP++                  +S  V   +  G+    L LEG G +D+
Sbjct: 61  FVFPPSEHENLPID------------------ESLFVAEQRKDGR---DLMLEGQGLSDQ 120

Query: 248 F---------SGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGE 307
           F         S  G++   SS+   H + FG  + + +  LVD E +      +S   GE
Sbjct: 121 FHYEAGNNQQSIYGESALGSSR---HMESFGSESAVYEHGLVDAEGN---LDLHSDGEGE 180

Query: 308 SDLSSTTFDEIPPLEPTDQVSAPEISETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEA 367
            D+  +T                               +LPC AWWKRRA S+YS  +EA
Sbjct: 181 KDVKKST------------------------------HNLPCEAWWKRRAISMYSRTREA 240

Query: 368 NAFWSIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGG 427
           NA WS+F AAAV GLV+LGQRWQQE WQ LQLKW  S+  +K  RVL P++RLKDVIV  
Sbjct: 241 NAIWSLFFAAAVTGLVVLGQRWQQERWQVLQLKWQSSISSEKLGRVLEPLSRLKDVIVRS 253

Query: 428 QRRSSSVKFSSS 430
             ++S V+  SS
Sbjct: 301 NPQASLVRSGSS 253

BLAST of Spg031782 vs. TAIR 10
Match: AT4G00355.3 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G45980.1); Has 100 Blast hits to 97 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 100; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 176.8 bits (447), Expect = 3.8e-44
Identity = 127/307 (41.37%), Postives = 161/307 (52.44%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVK---EGESTDENE-SNLYEAETSRALFM 187
           MAD D  E  +RGN+WEVVSLTASAY AAP  K   + +  D  E +  YEAETS  L+M
Sbjct: 1   MADKD--EAATRGNDWEVVSLTASAYAAAPGPKPVVDSKDDDHKEVTPCYEAETSHPLYM 60

Query: 188 SRHFVFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGE 247
           SRHFVFPP+                  G  EN  SD        S  K    L+L+GL  
Sbjct: 61  SRHFVFPPT------------------GQLEN-TSDLTEASLTGSHCKEGSDLSLKGLDL 120

Query: 248 TDEFSGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSST 307
           +D+F G+  + +K  K         EN     +S +D E+   G+  Y            
Sbjct: 121 SDDFGGLEFSEDKGKK--------EENIYTTAMSSLDDERAIGGSHVYE----------- 180

Query: 308 TFDEIPPLEPTDQVSAPEIS--ETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFW 367
                P  EPT+ VS  +++    P  D +   S     AWWKR  ASL + AKE N  W
Sbjct: 181 -----PVEEPTEPVSPSDVTLDLNPIKDDEVANSPPSEEAWWKRSVASLIAQAKETNTVW 240

Query: 368 SIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRS 427
           SI IAAAVMG+VILGQ WQQE WQ LQ KW  S+ ++K  R++GPI+RLK   VGGQRR 
Sbjct: 241 SICIAAAVMGIVILGQHWQQERWQILQQKWESSIGNEKAGRLMGPISRLKQAFVGGQRRD 262

Query: 428 SSVKFSS 429
           S ++ S+
Sbjct: 301 SFIRASA 262

BLAST of Spg031782 vs. TAIR 10
Match: AT4G00355.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G45980.1); Has 100 Blast hits to 97 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 100; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 176.8 bits (447), Expect = 3.8e-44
Identity = 127/307 (41.37%), Postives = 161/307 (52.44%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVK---EGESTDENE-SNLYEAETSRALFM 187
           MAD D  E  +RGN+WEVVSLTASAY AAP  K   + +  D  E +  YEAETS  L+M
Sbjct: 1   MADKD--EAATRGNDWEVVSLTASAYAAAPGPKPVVDSKDDDHKEVTPCYEAETSHPLYM 60

Query: 188 SRHFVFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGE 247
           SRHFVFPP+                  G  EN  SD        S  K    L+L+GL  
Sbjct: 61  SRHFVFPPT------------------GQLEN-TSDLTEASLTGSHCKEGSDLSLKGLDL 120

Query: 248 TDEFSGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSST 307
           +D+F G+  + +K  K         EN     +S +D E+   G+  Y            
Sbjct: 121 SDDFGGLEFSEDKGKK--------EENIYTTAMSSLDDERAIGGSHVYE----------- 180

Query: 308 TFDEIPPLEPTDQVSAPEIS--ETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFW 367
                P  EPT+ VS  +++    P  D +   S     AWWKR  ASL + AKE N  W
Sbjct: 181 -----PVEEPTEPVSPSDVTLDLNPIKDDEVANSPPSEEAWWKRSVASLIAQAKETNTVW 240

Query: 368 SIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRS 427
           SI IAAAVMG+VILGQ WQQE WQ LQ KW  S+ ++K  R++GPI+RLK   VGGQRR 
Sbjct: 241 SICIAAAVMGIVILGQHWQQERWQILQQKWESSIGNEKAGRLMGPISRLKQAFVGGQRRD 262

Query: 428 SSVKFSS 429
           S ++ S+
Sbjct: 301 SFIRASA 262

BLAST of Spg031782 vs. TAIR 10
Match: AT4G00355.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G45980.1); Has 100 Blast hits to 97 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 100; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 176.8 bits (447), Expect = 3.8e-44
Identity = 127/307 (41.37%), Postives = 161/307 (52.44%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVK---EGESTDENE-SNLYEAETSRALFM 187
           MAD D  E  +RGN+WEVVSLTASAY AAP  K   + +  D  E +  YEAETS  L+M
Sbjct: 1   MADKD--EAATRGNDWEVVSLTASAYAAAPGPKPVVDSKDDDHKEVTPCYEAETSHPLYM 60

Query: 188 SRHFVFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGE 247
           SRHFVFPP+                  G  EN  SD        S  K    L+L+GL  
Sbjct: 61  SRHFVFPPT------------------GQLEN-TSDLTEASLTGSHCKEGSDLSLKGLDL 120

Query: 248 TDEFSGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSST 307
           +D+F G+  + +K  K         EN     +S +D E+   G+  Y            
Sbjct: 121 SDDFGGLEFSEDKGKK--------EENIYTTAMSSLDDERAIGGSHVYE----------- 180

Query: 308 TFDEIPPLEPTDQVSAPEIS--ETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFW 367
                P  EPT+ VS  +++    P  D +   S     AWWKR  ASL + AKE N  W
Sbjct: 181 -----PVEEPTEPVSPSDVTLDLNPIKDDEVANSPPSEEAWWKRSVASLIAQAKETNTVW 240

Query: 368 SIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVHDQKTNRVLGPITRLKDVIVGGQRRS 427
           SI IAAAVMG+VILGQ WQQE WQ LQ KW  S+ ++K  R++GPI+RLK   VGGQRR 
Sbjct: 241 SICIAAAVMGIVILGQHWQQERWQILQQKWESSIGNEKAGRLMGPISRLKQAFVGGQRRD 262

Query: 428 SSVKFSS 429
           S ++ S+
Sbjct: 301 SFIRASA 262

BLAST of Spg031782 vs. TAIR 10
Match: AT4G00355.4 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G45980.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 143.7 bits (361), Expect = 3.5e-34
Identity = 111/277 (40.07%), Postives = 139/277 (50.18%), Query Frame = 0

Query: 128 MADNDGEENTSRGNEWEVVSLTASAYEAAPSVK---EGESTDENE-SNLYEAETSRALFM 187
           MAD D  E  +RGN+WEVVSLTASAY AAP  K   + +  D  E +  YEAETS  L+M
Sbjct: 1   MADKD--EAATRGNDWEVVSLTASAYAAAPGPKPVVDSKDDDHKEVTPCYEAETSHPLYM 60

Query: 188 SRHFVFPPSQHENLPLEPDKSEIHDDQGVKENVASDSAAVDGGKSSGKTEDSLNLEGLGE 247
           SRHFVFPP+                  G  EN  SD        S  K    L+L+GL  
Sbjct: 61  SRHFVFPPT------------------GQLEN-TSDLTEASLTGSHCKEGSDLSLKGLDL 120

Query: 248 TDEFSGIGKTMEKSSKLSFHGDDFGENTPLQDLSLVDKEQDPFGAPAYSSFHGESDLSST 307
           +D+F G+  + +K  K         EN     +S +D E+   G+  Y            
Sbjct: 121 SDDFGGLEFSEDKGKK--------EENIYTTAMSSLDDERAIGGSHVYE----------- 180

Query: 308 TFDEIPPLEPTDQVSAPEIS--ETPRLDSKSNKSSLPCGAWWKRRAASLYSHAKEANAFW 367
                P  EPT+ VS  +++    P  D +   S     AWWKR  ASL + AKE N  W
Sbjct: 181 -----PVEEPTEPVSPSDVTLDLNPIKDDEVANSPPSEEAWWKRSVASLIAQAKETNTVW 232

Query: 368 SIFIAAAVMGLVILGQRWQQESWQTLQLKWHISVHDQ 399
           SI IAAAVMG+VILGQ WQQE WQ LQ KW  S+ ++
Sbjct: 241 SICIAAAVMGIVILGQHWQQERWQILQQKWESSIGNE 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028597.11.6e-15384.57ATG8-interacting protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6597130.16.0e-15384.27ATG8-interacting protein 1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAE8650248.12.4e-14984.92hypothetical protein Csa_010335 [Cucumis sativus][more]
XP_023540318.15.8e-14889.14ATG8-interacting protein 1 [Cucurbita pepo subsp. pepo] >XP_023540319.1 ATG8-int... [more]
KAG6582617.11.0e-14771.57ATG8-interacting protein 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
O827757.1e-4841.99ATG8-interacting protein 1 OS=Arabidopsis thaliana OX=3702 GN=ATI1 PE=1 SV=2[more]
Q8VY985.3e-4341.37ATG8-interacting protein 2 OS=Arabidopsis thaliana OX=3702 GN=ATI2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L4834.5e-14687.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122320 PE=4 SV=1[more]
A0A1S3AVI52.5e-14486.51ATG8-interacting protein 1 OS=Cucumis melo OX=3656 GN=LOC103483267 PE=4 SV=1[more]
A0A5A7TZB92.5e-14486.51ATG8-interacting protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A6J1G3Q93.2e-14486.84ATG8-interacting protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450502 PE=4 SV... [more]
A0A6J1ITB63.1e-13985.53ATG8-interacting protein 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
AT2G45980.15.1e-4941.99unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT4G00355.33.8e-4441.37unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT4G00355.13.8e-4441.37unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT4G00355.23.8e-4441.37unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT4G00355.43.5e-3440.07unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 295..309
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 287..334
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 318..334
NoneNo IPR availablePANTHERPTHR34797:SF1ATG8-INTERACTING PROTEIN 2coord: 128..429
IPR040304ATG8-interacting protein 1/2PANTHERPTHR34797ATG8-INTERACTING PROTEIN 2coord: 128..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031782.1Spg031782.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle