Spg031720 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031720
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Beta-amylase
Location	scaffold11: 41059194 .. 41062356 (+)
RNA-Seq Expression	Spg031720
Synteny	Spg031720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACAAATCGAAAATCGTAAGAACAATCTTTGAATTTCTTCTTTTAAAATTGTTCATTGAAATTGGAATTCCAGTTTTGATTTCAGTTTGAATTTGGTCAACCATCTCGTTTGATTTTTATCGAATATGGCGTTGAGCATCACGCATCAGATTGGCGCGCTCGCCGGAACTCCGGTAGTCTCAGAAGCGAGTAACAACTCAGCCGGTGAAGCGATGGCTGCGGTGAATACGACGGCGTTGAGGAAGTCTCCGGCGTCGGCTCTAAGATGCAGAGTGCAGAGGACGGACGGAGTAGACGCGCTATCGCCGCCGGTGAGTCCGTGCAGATCGCCGGTGTTGGGAGGAATACGGCCGGATTTATCGGTGGCTTGCCAAGCGTTCGCGACGGAGGTGGAGGCTCCGACGGAAGTGAGGGAGTACAAGGAAGGAGGTGAGAGAGGGAAGGGGAAAGGAGTGCCGGTGTACGTGATGATGCCGTTGGACAGTGTGACGATGGGGAACACGGTGAATCGGAGGAAGGCGATGAACGCGAGTTTACAGGCGCTGAAGAGCGCCGGAGTGGAGGGGATTATGATGGACGTGTGGTGGGGATTGGTGGAGAGAGACTCGCCGGGTTCGTACAACTGGGGAGGCTACACGGAGCTTTTGGAAATGGCCAAAAAGCACGGCCTCAAAGTCCAAGCCGTCATGTCCTTCCATCAATGTGGAGGAAACGTTGGTGACTCTGTCACGTAAGCTCCTTCAACTTTTTCTATCTTTGTTTTCTTTTCAAGATTCGTATAATAATAGTTTAGTTGGGCACTTGCTCAAGTATTTCTGTGAAGGAATCGCTTGCCAAACTGGACTTCCATGAATCAATCCTGTTTCTCTGTTTACTCTGTTGCAATTAAGGATAATTACAATTCATGTTAGACTAAATCTATGTTTTAGTTTAAGAATTAGGGTACTTAATAGAGGAAATAATGAAATTTCTGCAAATTTGGAGGAAGAATTAGGAACTTTTTACTCCTGCTAAATTCTTCTTCAACAGTTCACTAGTATCAGATAAGTTTATCATCAATCAATGTAAGTTTATCATCTCATCATATGTCAATTTTGAAGGAAATAATATAATTGAGAATTGTTAAATGTGAGAAATAGATTTGAGATGTTCTTAGTGGGAGGGACTAAATTGATAATACCATCCAATACTTTAGGAATTAAAACATATATTTAGCCTTCGGATCGAGTATAAATGAACTAAGAAATCACTGGTTCTTTGCTGGGAATTTGGTGACAACTTTCTTTTCTACAGTTCTAATTTTGGGTGAGGTTAGGAAATGATATGGTGTTCTTTCTTATTTGGGTGCAGCATTCCTTTACCTAAATGGGCGGTGGAAGAGATTGATAAAGATCCAGATCTTGCATACACTGATCAATGGGGGAGAAGGAACTACGAATACATATCATTGGGATGTGACAACCTTCCTGTTCTCAAAGGCCGAACGCCCGTCCAGTGTTATGCAGACTTCATGCGTGCTTTCAGAGACAATTTCAAGCACCTTCTTGGTGACACCATTGTGGTAAAACCCTTGTTCATTTCCTTCTGCTTTGCCTGCGACTTCTTAATTGATGTGAATAATGGCTGAACTGTCTGGATTTTATTGTTTTGGTTTAGGAAATCCAAGTGGGAATGGGCCCAGCTGGTGAGCTCCGTTATCCATCCTACCCTGAGCAAGATGGGACATGGAAATTTCCAGGGATCGGAGCCTTCCAATGCTTTGACAAGGTATCTTCAACTTCCTGCTTCTTCATCTAACTTTTCCGCCAAGTTAGTTTGGTTTAGTGTAGTAATTTAACATGTAAAATGTCTATTCTTTCGATTGTCAGTTGTTGAGGTTTGTGGACTGTGGTAGTTTCATATCTCAAAAGTATATGAAAGGTTAAAATATGCCATAATCTATTAACATGATAGCTGGTTGTTGGGGTTTGTGCCAAATTTCTAAATCTCACAAGGCTATACAGATCTAAACATTATCATAATCTATTGACAATGGTCAGTTTTAGTTTAACATTTGCAATGGTGTGTGCAGTACATGCTGAGCAGTCTAAAAGCTGCTGCTGAAGCTGCTGGTAAACCTGAATGGGGAAGCACAGGTCCAACAGACGCCGGCGGCTACAACAACTGGCCAGAAGACACTCCATTTTTCCGAAAAGAAGGCGGTGGCTGGAACTCAACTTACGGCGAATTCTTCCTCTCCTGGTACTCCCAAATGCTGTTAGACCACGGAGATAGAATCCTCACAGCAGCCACTTCAACCTTCAAGAACACCGGCATCAAGATATCGGTGAAGATCGCAGGAATCCATTGGCACTACGGCCACCGCTCCCATGCCCCAGAGCTCACAGCAGGCTACTACAACACACGGTTCCGGGATGGCTATCTCCCAATCGCTCAAATGCTGGCACGCCATGGCGCCATCTTCAACTTCACCTGCATAGAAATGCGCGACCATGAGCAGCCACAAGACGCTCTCTGTGCACCTGAGAAGCTCGTCCGGCAGGTAGCTCTAGCCACCCACAAAGCCCAAGTCCCTCTGGCAGGCGAAAACGCTCTGCCTCGCTATGATGAGTTCGCCCATGACCAGATCCTGCAAGCTTCCTCTTTCAATGGCGACGGGGATGATTCAGAGGAGACTGAGATGTGTGCCTTCACTTATCTGAGAATGAACCCTCACCTGTTTGAGGCTGATAACTGGAGAAGATTCGTGGCGTTTGTGAAGAAGATGAAGGAAGGGAAGAATCCAGACAAGTGTTGGGAGCAAGTGGAGAGGGAAGCTGAGCATTTTGTTCATGTCACTCAACCTCTGGTTCAAGCGGCTGCTGTCGCTCTCATGCATTGACAAAAGAAAAAGGTTCGTTGTTTGTTATAGTGTTGTTGTATACTCCATAGTTGATGAATAGGTTCAATAATGGCGGTCTGATCTCGTCTGTTATCTACTCTGCTCTGCGTTTTCTGTAGCTCTCTGGTTTTTAGTTTGATGTAGCTATGGAAAAATGGAAATCTGTTGTAATTTCATTTCTATCCTTGTGATATGGGAAACTTGTTGTAAGAAAACCCTTCTCTCATGAGATGAGATTTGTATCTATTTTGATTTAAATGCTACTTTAGTCATACTTCGGAT

mRNA sequence

ATGGCGTTGAGCATCACGCATCAGATTGGCGCGCTCGCCGGAACTCCGGTAGTCTCAGAAGCGAGTAACAACTCAGCCGGTGAAGCGATGGCTGCGGTGAATACGACGGCGTTGAGGAAGTCTCCGGCGTCGGCTCTAAGATGCAGAGTGCAGAGGACGGACGGAGTAGACGCGCTATCGCCGCCGGTGAGTCCGTGCAGATCGCCGGTGTTGGGAGGAATACGGCCGGATTTATCGGTGGCTTGCCAAGCGTTCGCGACGGAGGTGGAGGCTCCGACGGAAGTGAGGGAGTACAAGGAAGGAGGTGAGAGAGGGAAGGGGAAAGGAGTGCCGGTGTACGTGATGATGCCGTTGGACAGTGTGACGATGGGGAACACGGTGAATCGGAGGAAGGCGATGAACGCGAGTTTACAGGCGCTGAAGAGCGCCGGAGTGGAGGGGATTATGATGGACGTGTGGTGGGGATTGGTGGAGAGAGACTCGCCGGGTTCGTACAACTGGGGAGGCTACACGGAGCTTTTGGAAATGGCCAAAAAGCACGGCCTCAAAGTCCAAGCCGTCATGTCCTTCCATCAATGTGGAGGAAACGTTGGTGACTCTGTCACCATTCCTTTACCTAAATGGGCGGTGGAAGAGATTGATAAAGATCCAGATCTTGCATACACTGATCAATGGGGGAGAAGGAACTACGAATACATATCATTGGGATGTGACAACCTTCCTGTTCTCAAAGGCCGAACGCCCGTCCAGTGTTATGCAGACTTCATGCGTGCTTTCAGAGACAATTTCAAGCACCTTCTTGGTGACACCATTGTGGAAATCCAAGTGGGAATGGGCCCAGCTGGTGAGCTCCGTTATCCATCCTACCCTGAGCAAGATGGGACATGGAAATTTCCAGGGATCGGAGCCTTCCAATGCTTTGACAAGTACATGCTGAGCAGTCTAAAAGCTGCTGCTGAAGCTGCTGGTAAACCTGAATGGGGAAGCACAGGTCCAACAGACGCCGGCGGCTACAACAACTGGCCAGAAGACACTCCATTTTTCCGAAAAGAAGGCGGTGGCTGGAACTCAACTTACGGCGAATTCTTCCTCTCCTGGTACTCCCAAATGCTGTTAGACCACGGAGATAGAATCCTCACAGCAGCCACTTCAACCTTCAAGAACACCGGCATCAAGATATCGGTGAAGATCGCAGGAATCCATTGGCACTACGGCCACCGCTCCCATGCCCCAGAGCTCACAGCAGGCTACTACAACACACGGTTCCGGGATGGCTATCTCCCAATCGCTCAAATGCTGGCACGCCATGGCGCCATCTTCAACTTCACCTGCATAGAAATGCGCGACCATGAGCAGCCACAAGACGCTCTCTGTGCACCTGAGAAGCTCGTCCGGCAGGTAGCTCTAGCCACCCACAAAGCCCAAGTCCCTCTGGCAGGCGAAAACGCTCTGCCTCGCTATGATGAGTTCGCCCATGACCAGATCCTGCAAGCTTCCTCTTTCAATGGCGACGGGGATGATTCAGAGGAGACTGAGATGTGTGCCTTCACTTATCTGAGAATGAACCCTCACCTGTTTGAGGCTGATAACTGGAGAAGATTCGTGGCGTTTGTGAAGAAGATGAAGGAAGGGAAGAATCCAGACAAGTGTTGGGAGCAAGTGGAGAGGGAAGCTGAGCATTTTGTTCATGTCACTCAACCTCTGGTTCAAGCGGCTGCTGTCGCTCTCATGCATTGA

Coding sequence (CDS)

ATGGCGTTGAGCATCACGCATCAGATTGGCGCGCTCGCCGGAACTCCGGTAGTCTCAGAAGCGAGTAACAACTCAGCCGGTGAAGCGATGGCTGCGGTGAATACGACGGCGTTGAGGAAGTCTCCGGCGTCGGCTCTAAGATGCAGAGTGCAGAGGACGGACGGAGTAGACGCGCTATCGCCGCCGGTGAGTCCGTGCAGATCGCCGGTGTTGGGAGGAATACGGCCGGATTTATCGGTGGCTTGCCAAGCGTTCGCGACGGAGGTGGAGGCTCCGACGGAAGTGAGGGAGTACAAGGAAGGAGGTGAGAGAGGGAAGGGGAAAGGAGTGCCGGTGTACGTGATGATGCCGTTGGACAGTGTGACGATGGGGAACACGGTGAATCGGAGGAAGGCGATGAACGCGAGTTTACAGGCGCTGAAGAGCGCCGGAGTGGAGGGGATTATGATGGACGTGTGGTGGGGATTGGTGGAGAGAGACTCGCCGGGTTCGTACAACTGGGGAGGCTACACGGAGCTTTTGGAAATGGCCAAAAAGCACGGCCTCAAAGTCCAAGCCGTCATGTCCTTCCATCAATGTGGAGGAAACGTTGGTGACTCTGTCACCATTCCTTTACCTAAATGGGCGGTGGAAGAGATTGATAAAGATCCAGATCTTGCATACACTGATCAATGGGGGAGAAGGAACTACGAATACATATCATTGGGATGTGACAACCTTCCTGTTCTCAAAGGCCGAACGCCCGTCCAGTGTTATGCAGACTTCATGCGTGCTTTCAGAGACAATTTCAAGCACCTTCTTGGTGACACCATTGTGGAAATCCAAGTGGGAATGGGCCCAGCTGGTGAGCTCCGTTATCCATCCTACCCTGAGCAAGATGGGACATGGAAATTTCCAGGGATCGGAGCCTTCCAATGCTTTGACAAGTACATGCTGAGCAGTCTAAAAGCTGCTGCTGAAGCTGCTGGTAAACCTGAATGGGGAAGCACAGGTCCAACAGACGCCGGCGGCTACAACAACTGGCCAGAAGACACTCCATTTTTCCGAAAAGAAGGCGGTGGCTGGAACTCAACTTACGGCGAATTCTTCCTCTCCTGGTACTCCCAAATGCTGTTAGACCACGGAGATAGAATCCTCACAGCAGCCACTTCAACCTTCAAGAACACCGGCATCAAGATATCGGTGAAGATCGCAGGAATCCATTGGCACTACGGCCACCGCTCCCATGCCCCAGAGCTCACAGCAGGCTACTACAACACACGGTTCCGGGATGGCTATCTCCCAATCGCTCAAATGCTGGCACGCCATGGCGCCATCTTCAACTTCACCTGCATAGAAATGCGCGACCATGAGCAGCCACAAGACGCTCTCTGTGCACCTGAGAAGCTCGTCCGGCAGGTAGCTCTAGCCACCCACAAAGCCCAAGTCCCTCTGGCAGGCGAAAACGCTCTGCCTCGCTATGATGAGTTCGCCCATGACCAGATCCTGCAAGCTTCCTCTTTCAATGGCGACGGGGATGATTCAGAGGAGACTGAGATGTGTGCCTTCACTTATCTGAGAATGAACCCTCACCTGTTTGAGGCTGATAACTGGAGAAGATTCGTGGCGTTTGTGAAGAAGATGAAGGAAGGGAAGAATCCAGACAAGTGTTGGGAGCAAGTGGAGAGGGAAGCTGAGCATTTTGTTCATGTCACTCAACCTCTGGTTCAAGCGGCTGCTGTCGCTCTCATGCATTGA

Protein sequence

MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALSPPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDSVTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNLPVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYGEFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAGENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKKMKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH

Homology

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BLAST of Spg031720 vs. NCBI nr
Match: XP_008438436.1 (PREDICTED: beta-amylase 1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 543/578 (93.94%), Postives = 564/578 (97.58%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MALSITHQIGALAGTP+ SEASN SAGEA AAVNT  LRKS +S LRCRVQRT+GVDALS
Sbjct: 1   MALSITHQIGALAGTPMTSEASNTSAGEASAAVNTAVLRKSASSPLRCRVQRTEGVDALS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGGIRPDLSVACQAFATEV APTEVREYKEGGE+GK KGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGIRPDLSVACQAFATEVAAPTEVREYKEGGEKGKEKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEE+DKDPDLAYTDQWGRRNYEYISLGCD L
Sbjct: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEMDKDPDLAYTDQWGRRNYEYISLGCDTL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAF+DNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTW+FPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFKDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWRFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEA+GKPEWGSTGPTDAGGYNNWPEDTPFFRK+GGGWNSTYG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEASGKPEWGSTGPTDAGGYNNWPEDTPFFRKDGGGWNSTYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFL+WYSQMLLDHG+RILTAATSTF+NTG+KISVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLTWYSQMLLDHGERILTAATSTFENTGVKISVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           R+RDGYLPIA+MLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVA ATHKAQVPLAG
Sbjct: 421 RYRDGYLPIARMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVAQATHKAQVPLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDEFAH+QILQASSF GD +DSEE+EMCAFTYLRMNPHLFEA+NWRRFVAFVKK
Sbjct: 481 ENALPRYDEFAHEQILQASSFIGD-EDSEESEMCAFTYLRMNPHLFEAENWRRFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNPDKCWEQVEREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. NCBI nr
Match: XP_022980262.1 (beta-amylase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 544/578 (94.12%), Postives = 558/578 (96.54%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MA SITHQIGALAGTPV SE SN ++GEA AAVN   LRKSPASALRCRVQRTDGVDALS
Sbjct: 1   MAFSITHQIGALAGTPVTSEMSNGASGEATAAVNAAVLRKSPASALRCRVQRTDGVDALS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGGIRPDLSVACQAFATEV APTEV+EYKEGGERGKGKGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGIRPDLSVACQAFATEVAAPTEVKEYKEGGERGKGKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMSFHQCGGNVGDSVTIPLPKW VEEIDKDPDLAYTDQWGRRNYEYISLGCDNL
Sbjct: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWVVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAF+DNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTWKFPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFKDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWKFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEA GKPEWG TGPTDAG YNNWPEDTPFFRK+GGGWNSTYG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEAYGKPEWGVTGPTDAGSYNNWPEDTPFFRKDGGGWNSTYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFLSWYSQMLL HG+RIL AATSTF+NTG+K+SVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLSWYSQMLLAHGERILMAATSTFENTGVKMSVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG
Sbjct: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDE AH QILQASSF G+G DSEETEMCAFTYLRMNPHLFEA+NWRRFVAFVKK
Sbjct: 481 ENALPRYDESAHGQILQASSFIGEG-DSEETEMCAFTYLRMNPHLFEAENWRRFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNPDKCWEQ+EREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPDKCWEQLEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. NCBI nr
Match: KAG6582430.1 (Beta-amylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 543/578 (93.94%), Postives = 558/578 (96.54%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MA SITHQIGALAGTPV SE SN ++GEA AAVN   LRKSPAS LRCRVQRTDGVDALS
Sbjct: 1   MAFSITHQIGALAGTPVTSEMSNGASGEATAAVNAAVLRKSPASTLRCRVQRTDGVDALS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGGIRPDLSVACQAFATEV APTEV+EYKEGGERGKGKGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGIRPDLSVACQAFATEVAAPTEVKEYKEGGERGKGKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVER+SPGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERESPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMSFHQCGGNVGDSVTIPLPKW VEEIDKDPDLAYTDQWGRRNYEYISLGCDNL
Sbjct: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWVVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAF++NFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTWKFPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFKENFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWKFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEA GKPEWG TGPTDAG YNNWPEDTPFFRK+GGGWNSTYG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEAFGKPEWGVTGPTDAGSYNNWPEDTPFFRKDGGGWNSTYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFLSWYSQMLL HG+RILTAATSTF+NTG+KISVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLSWYSQMLLAHGERILTAATSTFENTGVKISVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG
Sbjct: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDE AH QILQASSF G+G DSEETEMCAFTYLRMNPHLFEA+NWRRFVAFVKK
Sbjct: 481 ENALPRYDESAHGQILQASSFIGEG-DSEETEMCAFTYLRMNPHLFEAENWRRFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNPDKCWEQ+EREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPDKCWEQLEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. NCBI nr
Match: XP_022924792.1 (beta-amylase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 543/578 (93.94%), Postives = 557/578 (96.37%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MA SITHQIGALAGTPV SE SN ++GEA AAVN   LRKSPAS LRCRVQRTDGVDALS
Sbjct: 1   MAFSITHQIGALAGTPVTSEMSNGASGEATAAVNAAVLRKSPASTLRCRVQRTDGVDALS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGGIRPDLSVACQAFATEV APTEV+EYKEGGERGKGKGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGIRPDLSVACQAFATEVAAPTEVKEYKEGGERGKGKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMSFHQCGGNVGDSVTIPLPKW VEEIDKDPDLAYTDQWGRRNYEYISLGCDNL
Sbjct: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWVVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAF++NFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTWKFPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFKENFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWKFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEA GKPEWG TGPTDAG YNNWPEDTPFFRK+GGGWNSTYG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEAFGKPEWGVTGPTDAGSYNNWPEDTPFFRKDGGGWNSTYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFLSWYSQMLL HG+RILTAATSTF+NTG+KISVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLSWYSQMLLAHGERILTAATSTFENTGVKISVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG
Sbjct: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDE AH QILQASSF G+G DSEE EMCAFTYLRMNPHLFEA+NWRRFVAFVKK
Sbjct: 481 ENALPRYDESAHGQILQASSFIGEG-DSEEAEMCAFTYLRMNPHLFEAENWRRFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNPDKCWEQ+EREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPDKCWEQLEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. NCBI nr
Match: KAG7018828.1 (Beta-amylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 541/578 (93.60%), Postives = 557/578 (96.37%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MA SITHQIGALAGTPV SE SN ++GEA AAVN   LRKSPAS LRCRVQRTDGVDALS
Sbjct: 1   MAFSITHQIGALAGTPVTSEMSNGASGEATAAVNAAVLRKSPASTLRCRVQRTDGVDALS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGGIRPDLSVACQAFATEV APTEV+EYKEGGERGK KGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGIRPDLSVACQAFATEVAAPTEVKEYKEGGERGKAKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVER+SPGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERESPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMSFHQCGGNVGDSVTIPLPKW VEEIDKDPDLAYTDQWGRRNYEY+SLGCDNL
Sbjct: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWVVEEIDKDPDLAYTDQWGRRNYEYVSLGCDNL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAF++NFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTWKFPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFKENFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWKFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEA GKPEWG TGPTDAG YNNWPEDTPFFRK+GGGWNSTYG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEAFGKPEWGVTGPTDAGSYNNWPEDTPFFRKDGGGWNSTYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFLSWYSQMLL HG+RILTAATSTF+NTG+KISVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLSWYSQMLLAHGERILTAATSTFENTGVKISVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG
Sbjct: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDE AH QILQASSF G+G DSEETEMCAFTYLRMNPHLFEA+NWRRFVAFVKK
Sbjct: 481 ENALPRYDESAHGQILQASSFIGEG-DSEETEMCAFTYLRMNPHLFEAENWRRFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNPDKCWEQ+EREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPDKCWEQLEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. ExPASy Swiss-Prot
Match: Q9LIR6 (Beta-amylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM1 PE=1 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 1.4e-264
Identity = 448/584 (76.71%), Postives = 500/584 (85.62%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MAL+++HQ+G LAGTP+ S    +S   ++ +++  + R     A+  R  +  G D  S
Sbjct: 1   MALNLSHQLGVLAGTPIKSGEMTDS---SLLSISPPSARMMTPKAMN-RNYKAHGTDP-S 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATE--VEAPTEVREYKEGGERGK---GKGVPVYVM 120
           PP+    SP+LG  R DLSVAC+AFA E  +    E R Y+EGG  GK   G GVPV+VM
Sbjct: 61  PPM----SPILGATRADLSVACKAFAVENGIGTIEEQRTYREGGIGGKKEGGGGVPVFVM 120

Query: 121 MPLDSVTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLE 180
           MPLDSVTMGNTVNRRKAM ASLQALKSAGVEGIM+DVWWGLVE++SPG+YNWGGY ELLE
Sbjct: 121 MPLDSVTMGNTVNRRKAMKASLQALKSAGVEGIMIDVWWGLVEKESPGTYNWGGYNELLE 180

Query: 181 MAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISL 240
           +AKK GLKVQAVMSFHQCGGNVGDSVTIPLP+W VEE+DKDPDLAYTDQWGRRN+EYISL
Sbjct: 181 LAKKLGLKVQAVMSFHQCGGNVGDSVTIPLPQWVVEEVDKDPDLAYTDQWGRRNHEYISL 240

Query: 241 GCDNLPVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGT 300
           G D LPVLKGRTPVQCYADFMRAFRDNFKHLLG+TIVEIQVGMGPAGELRYPSYPEQ+GT
Sbjct: 241 GADTLPVLKGRTPVQCYADFMRAFRDNFKHLLGETIVEIQVGMGPAGELRYPSYPEQEGT 300

Query: 301 WKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGW 360
           WKFPGIGAFQC+DKY LSSLKAAAE  GKPEWGSTGPTDAG YNNWPEDT FF+KEGGGW
Sbjct: 301 WKFPGIGAFQCYDKYSLSSLKAAAETYGKPEWGSTGPTDAGHYNNWPEDTQFFKKEGGGW 360

Query: 361 NSTYGEFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTA 420
           NS YG+FFLSWYSQMLLDHG+RIL++A S F+N G+KISVKIAGIHWHYG RSHAPELTA
Sbjct: 361 NSEYGDFFLSWYSQMLLDHGERILSSAKSIFENMGVKISVKIAGIHWHYGTRSHAPELTA 420

Query: 421 GYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQ 480
           GYYNTRFRDGYLPIAQMLARH AIFNFTCIEMRDHEQPQDALCAPEKLV QVALAT  A+
Sbjct: 421 GYYNTRFRDGYLPIAQMLARHNAIFNFTCIEMRDHEQPQDALCAPEKLVNQVALATLAAE 480

Query: 481 VPLAGENALPRYDEFAHDQILQASSFNGD-GDDSEETEMCAFTYLRMNPHLFEADNWRRF 540
           VPLAGENALPRYD++AH+QIL+AS+ N D  ++ E  EMCAFTYLRMNP LF+ADNW +F
Sbjct: 481 VPLAGENALPRYDDYAHEQILKASALNLDQNNEGEPREMCAFTYLRMNPELFQADNWGKF 540

Query: 541 VAFVKKMKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           VAFVKKM EG++  +C E+VEREAEHFVHVTQPLVQ AAVAL H
Sbjct: 541 VAFVKKMGEGRDSHRCREEVEREAEHFVHVTQPLVQEAAVALTH 575

[Close]

BLAST of Spg031720 vs. ExPASy Swiss-Prot
Match: Q10RZ1 (Beta-amylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY2 PE=1 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 1.2e-212
Identity = 376/578 (65.05%), Postives = 445/578 (76.99%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           M+L++ HQ GA A    V+ A+  +A  A AA   +A   +PA+A   ++Q T  VD   
Sbjct: 2   MSLNLAHQTGAAA---AVAPAAPRTAVVAAAAGTVSAPAVAPAAAPSLQLQ-TQTVD--- 61

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
            P +P +        PDL +A QA    VE+  E +    GGE  +  GVPVYVMMPLD+
Sbjct: 62  -PAAPAQG-------PDLPMAFQAL---VESLPEEQHPDVGGEERRKVGVPVYVMMPLDT 121

Query: 121 VTM-GNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKK 180
           V   GN +NRRKA+ ASL+ALKSAG EGIM+DVWWG+ E + PG YN+ GY EL+EMAKK
Sbjct: 122 VRKDGNGLNRRKAVEASLKALKSAGAEGIMVDVWWGIAECEGPGRYNFTGYMELMEMAKK 181

Query: 181 HGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDN 240
           +GLKVQAVMSFHQCGGNVGDSVTIPLPKW +EE+DKD DLAYTD+ GRRNYEY+SLG D 
Sbjct: 182 NGLKVQAVMSFHQCGGNVGDSVTIPLPKWVLEEMDKDQDLAYTDRSGRRNYEYLSLGADA 241

Query: 241 LPVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFP 300
           +PVLKGRTPVQCY DFMRAFRD+F   +G+TIVEIQVGMGPAGELRYPSYPE +GTW+FP
Sbjct: 242 MPVLKGRTPVQCYGDFMRAFRDHFAAFMGNTIVEIQVGMGPAGELRYPSYPESNGTWRFP 301

Query: 301 GIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTY 360
           GIG FQC+D+YMLSSLKAAAEA GKPEWG+ GP D+GGYN+WPED+PFFR+E GGWN+ Y
Sbjct: 302 GIGEFQCYDRYMLSSLKAAAEAVGKPEWGNAGPGDSGGYNDWPEDSPFFRRE-GGWNTPY 361

Query: 361 GEFFLSWYSQMLLDHGDRILTAATSTFKNT-GIKISVKIAGIHWHYGHRSHAPELTAGYY 420
           GEFF+SWYSQMLL+HG+RIL+AA+  +  T G+KISVK+AGIHWHYG RSHA ELTAGYY
Sbjct: 362 GEFFMSWYSQMLLEHGERILSAASGVYTGTPGVKISVKVAGIHWHYGTRSHAAELTAGYY 421

Query: 421 NTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPL 480
           NTR  DGY PIA+MLARHGA+ NFTC+EMR+HEQPQDA C PE+LV+QVA A  ++ V L
Sbjct: 422 NTRHHDGYQPIARMLARHGAVLNFTCVEMRNHEQPQDAQCRPEELVQQVAAAARESGVGL 481

Query: 481 AGENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFV 540
           AGENALPRYDE AHDQI+  ++     + +EE  M AFTYLRM P LF+ DNWRRF AFV
Sbjct: 482 AGENALPRYDETAHDQIVTTAA-----EKAEEERMVAFTYLRMGPDLFQPDNWRRFAAFV 541

Query: 541 KKMKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVAL 577
           K+M E    D C EQVEREA+   H T  LV  AAVAL
Sbjct: 542 KRMTESGVRDVCREQVEREAQGVAHATGSLVHEAAVAL 555

[Close]

BLAST of Spg031720 vs. ExPASy Swiss-Prot
Match: Q9AV88 (Beta-amylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY1 PE=1 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 2.8e-204
Identity = 355/537 (66.11%), Postives = 401/537 (74.67%), Query Frame = 0


Query: 43  ASALRCRVQRTDGVDALSPPVSP-CRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEG 102
           A+A  C     D   A S    P   S     +R     AC+  A    A    R     
Sbjct: 9   AAAAACFATAGDARRAASVVAMPSSSSSATTSLRMKRQAACEPVACRAVA----RHVAAA 68

Query: 103 GERGKGKGVPVYVMMPLDSVT-MGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERD 162
               +  GVPV+VMMPLD+V+  G+ +NRRKA+ ASL ALKSAGVEGIM+DVWWG+VE +
Sbjct: 69  AASSRRNGVPVFVMMPLDTVSKCGSALNRRKAVAASLAALKSAGVEGIMVDVWWGIVESE 128

Query: 163 SPGSYNWGGYTELLEMAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLA 222
            PG YN+ GY EL+EMA+K GLKVQAVMSFHQCGGNVGDSV IPLP+W VEE++KD DLA
Sbjct: 129 GPGRYNFDGYVELMEMARKTGLKVQAVMSFHQCGGNVGDSVNIPLPRWVVEEMEKDNDLA 188

Query: 223 YTDQWGRRNYEYISLGCDNLPVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGP 282
           YTDQWGRRN+EYISLGCD +PV KGRTPV+CY DFMRAFRD+F   LGDTIVEIQVGMGP
Sbjct: 189 YTDQWGRRNFEYISLGCDAMPVFKGRTPVECYTDFMRAFRDHFASFLGDTIVEIQVGMGP 248

Query: 283 AGELRYPSYPEQDGTWKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNN 342
           AGELRYPSYPE +GTW+FPGIGAFQC D+YM SSLKAAAEA GKPEWG  GPTDAGGYNN
Sbjct: 249 AGELRYPSYPESNGTWRFPGIGAFQCNDRYMRSSLKAAAEARGKPEWGHGGPTDAGGYNN 308

Query: 343 WPEDTPFFRKEGGGWNSTYGEFFLSWYSQMLLDHGDRILTAATSTF-KNTGIKISVKIAG 402
           WPEDT FFR + GGW++ YGEFFLSWYSQMLL+HG+R+L+ ATS F    G KISVK+AG
Sbjct: 309 WPEDTVFFRGDCGGWSTEYGEFFLSWYSQMLLEHGERVLSGATSVFGDGAGAKISVKVAG 368

Query: 403 IHWHYGHRSHAPELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCA 462
           IHWHYG RSHAPELTAGYYNTR RDGYLPIA+MLARHGA+ NFTC+EMRDHEQPQ+A C 
Sbjct: 369 IHWHYGTRSHAPELTAGYYNTRHRDGYLPIARMLARHGAVLNFTCVEMRDHEQPQEAQCM 428

Query: 463 PEKLVRQVALATHKAQVPLAGENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYL 522
           PE LVRQVA A   A V LAGENALPRYD  AHDQ++ A++     D + E  M AFTYL
Sbjct: 429 PEALVRQVAAAARAAGVGLAGENALPRYDGTAHDQVVAAAA-----DRAAEDRMVAFTYL 488

Query: 523 RMNPHLFEADNWRRFVAFVKKMKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVAL 577
           RM P LF  DNWRRFVAFV++M E  +P    E  E  A      T  LV  AAVAL
Sbjct: 489 RMGPDLFHPDNWRRFVAFVRRMSESGSPR---EAAESAAHGVAQATGSLVHEAAVAL 533

[Close]

BLAST of Spg031720 vs. ExPASy Swiss-Prot
Match: O23553 (Beta-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM3 PE=1 SV=3)

HSP 1 Score: 603.2 bits (1554), Expect = 3.1e-171
Identity = 277/436 (63.53%), Postives = 341/436 (78.21%), Query Frame = 0


Query: 110 VPVYVMMPLDSVTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGG 169
           VPV+VM+PLD+VTM   +N+ +AMNASL ALK AGVEG+M+D WWGLVE+D P +YNW G
Sbjct: 86  VPVFVMLPLDTVTMSGHLNKPRAMNASLMALKGAGVEGVMVDAWWGLVEKDGPMNYNWEG 145

Query: 170 YTELLEMAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRN 229
           Y EL++M +KHGLK+Q VMSFHQCGGNVGDS +IPLP W +EEI K+PDL YTD+ GRRN
Sbjct: 146 YAELIQMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDKSGRRN 205

Query: 230 YEYISLGCDNLPVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSY 289
            EYISLGCD++PVL+GRTP+Q Y+DFMR+FR+ F+  +G  I EIQVGMGP GELRYPSY
Sbjct: 206 PEYISLGCDSVPVLRGRTPIQVYSDFMRSFRERFEGYIGGVIAEIQVGMGPCGELRYPSY 265

Query: 290 PEQDGTWKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFR 349
           PE +GTW+FPGIG FQC+DKYM SSL+A AE+ GK  WG++GP DAG Y N PEDT FFR
Sbjct: 266 PESNGTWRFPGIGEFQCYDKYMKSSLQAYAESIGKTNWGTSGPHDAGEYKNLPEDTEFFR 325

Query: 350 KEGGGWNSTYGEFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSH 409
           ++ G WNS YG+FF+ WYS  LL+HGD++L++A   F+ +G K+S K+AGIHWHY  RSH
Sbjct: 326 RD-GTWNSEYGKFFMEWYSGKLLEHGDQLLSSAKGIFQGSGAKLSGKVAGIHWHYNTRSH 385

Query: 410 APELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVAL 469
           A ELTAGYYNTR  DGYLPIA+M  +HG + NFTC+EM+D EQP+ A C+PE LV+QV  
Sbjct: 386 AAELTAGYYNTRNHDGYLPIAKMFNKHGVVLNFTCMEMKDGEQPEHANCSPEGLVKQVQN 445

Query: 470 ATHKAQVPLAGENALPRYDEFAHDQILQAS-SFNGDGDDSEETEMCAFTYLRMNPHLFEA 529
           AT +A   LAGENAL RYD  A  Q++  + S +G+G       + AFTYLRMN  LFE 
Sbjct: 446 ATRQAGTELAGENALERYDSSAFGQVVATNRSDSGNG-------LTAFTYLRMNKRLFEG 505

Query: 530 DNWRRFVAFVKKMKEG 545
            NW++ V FVK MKEG
Sbjct: 506 QNWQQLVEFVKNMKEG 513

[Close]

BLAST of Spg031720 vs. ExPASy Swiss-Prot
Match: P82993 (Beta-amylase OS=Hordeum vulgare subsp. spontaneum OX=77009 GN=BMY1 PE=1 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 7.2e-128
Identity = 226/438 (51.60%), Postives = 295/438 (67.35%), Query Frame = 0


Query: 106 KGKGVPVYVMMPLDSVTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSY 165
           KG  V VYVM+PLD+V++ N   +   + A L+ L  AGV+G+M+DVWWGLVE   P +Y
Sbjct: 6   KGNYVQVYVMLPLDAVSVNNRFEKGDELRAQLRKLVEAGVDGVMVDVWWGLVEGKGPKAY 65

Query: 166 NWGGYTELLEMAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQW 225
           +W  Y +L E+ +K GLK+QA+MSFHQCGGNVGD+V IP+P+W  +   +DPD+ YTD  
Sbjct: 66  DWSAYKQLFELVQKAGLKLQAIMSFHQCGGNVGDAVNIPIPQWVRDVGTRDPDIFYTDGH 125

Query: 226 GRRNYEYISLGCDNLPVLKGRTPVQCYADFMRAFRDNFKHLL-GDTIVEIQVGMGPAGEL 285
           G RN EY++LG DN P+  GR+ VQ YAD+M +FR+N K  L    IV+I+VG+GPAGE+
Sbjct: 126 GTRNIEYLTLGVDNQPLFHGRSAVQMYADYMTSFRENMKEFLDAGVIVDIEVGLGPAGEM 185

Query: 286 RYPSYPEQDGTWKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPED 345
           RYPSYP+  G W FPGIG F C+DKY+ +  KAAA A G PEW    P DAG YN+ PE 
Sbjct: 186 RYPSYPQSHG-WSFPGIGEFICYDKYLQADFKAAAAAVGHPEW--EFPNDAGQYNDTPER 245

Query: 346 TPFFRKEGGGWNSTYGEFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHY 405
           T FFR + G + +  G FFL+WYS  L+ HGDRIL  A   F    +++++KI+GIHW Y
Sbjct: 246 TQFFR-DNGTYLTEKGRFFLAWYSNNLIKHGDRILDEANKVFLGYKVQLAIKISGIHWWY 305

Query: 406 GHRSHAPELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLV 465
              SHA ELTAGYYN   RDGY  IA+ML RH A  NFTC EMRD EQ   A+ APE+LV
Sbjct: 306 KVPSHAAELTAGYYNLHDRDGYRTIARMLKRHRASINFTCAEMRDSEQSSQAMSAPEELV 365

Query: 466 RQVALATHKAQVPLAGENALPRYDEFAHDQILQASSFNG-DGDDSEETEMCAFTYLRMNP 525
           +QV  A  +  + +A ENALPRYD  A++ IL+ +  +G +     E ++  FTYLR++ 
Sbjct: 366 QQVLSAGWREGLNVACENALPRYDPTAYNTILRNARPHGINQSGPPEHKLFGFTYLRLSN 425

Query: 526 HLFEADNWRRFVAFVKKM 542
            L E  N+  F  FV +M
Sbjct: 426 QLVEGQNYVNFKTFVDRM 439

[Close]

BLAST of Spg031720 vs. ExPASy TrEMBL
Match: A0A1S3AWG6 (Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103483532 PE=3 SV=1)

HSP 1 Score: 1137.9 bits (2942), Expect = 0.0e+00
Identity = 543/578 (93.94%), Postives = 564/578 (97.58%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MALSITHQIGALAGTP+ SEASN SAGEA AAVNT  LRKS +S LRCRVQRT+GVDALS
Sbjct: 1   MALSITHQIGALAGTPMTSEASNTSAGEASAAVNTAVLRKSASSPLRCRVQRTEGVDALS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGGIRPDLSVACQAFATEV APTEVREYKEGGE+GK KGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGIRPDLSVACQAFATEVAAPTEVREYKEGGEKGKEKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEE+DKDPDLAYTDQWGRRNYEYISLGCD L
Sbjct: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEMDKDPDLAYTDQWGRRNYEYISLGCDTL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAF+DNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTW+FPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFKDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWRFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEA+GKPEWGSTGPTDAGGYNNWPEDTPFFRK+GGGWNSTYG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEASGKPEWGSTGPTDAGGYNNWPEDTPFFRKDGGGWNSTYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFL+WYSQMLLDHG+RILTAATSTF+NTG+KISVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLTWYSQMLLDHGERILTAATSTFENTGVKISVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           R+RDGYLPIA+MLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVA ATHKAQVPLAG
Sbjct: 421 RYRDGYLPIARMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVAQATHKAQVPLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDEFAH+QILQASSF GD +DSEE+EMCAFTYLRMNPHLFEA+NWRRFVAFVKK
Sbjct: 481 ENALPRYDEFAHEQILQASSFIGD-EDSEESEMCAFTYLRMNPHLFEAENWRRFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNPDKCWEQVEREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. ExPASy TrEMBL
Match: A0A6J1IQX2 (Beta-amylase OS=Cucurbita maxima OX=3661 GN=LOC111479682 PE=3 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 544/578 (94.12%), Postives = 558/578 (96.54%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MA SITHQIGALAGTPV SE SN ++GEA AAVN   LRKSPASALRCRVQRTDGVDALS
Sbjct: 1   MAFSITHQIGALAGTPVTSEMSNGASGEATAAVNAAVLRKSPASALRCRVQRTDGVDALS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGGIRPDLSVACQAFATEV APTEV+EYKEGGERGKGKGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGIRPDLSVACQAFATEVAAPTEVKEYKEGGERGKGKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMSFHQCGGNVGDSVTIPLPKW VEEIDKDPDLAYTDQWGRRNYEYISLGCDNL
Sbjct: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWVVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAF+DNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTWKFPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFKDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWKFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEA GKPEWG TGPTDAG YNNWPEDTPFFRK+GGGWNSTYG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEAYGKPEWGVTGPTDAGSYNNWPEDTPFFRKDGGGWNSTYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFLSWYSQMLL HG+RIL AATSTF+NTG+K+SVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLSWYSQMLLAHGERILMAATSTFENTGVKMSVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG
Sbjct: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDE AH QILQASSF G+G DSEETEMCAFTYLRMNPHLFEA+NWRRFVAFVKK
Sbjct: 481 ENALPRYDESAHGQILQASSFIGEG-DSEETEMCAFTYLRMNPHLFEAENWRRFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNPDKCWEQ+EREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPDKCWEQLEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. ExPASy TrEMBL
Match: A0A6J1E9Z7 (Beta-amylase OS=Cucurbita moschata OX=3662 GN=LOC111432184 PE=3 SV=1)

HSP 1 Score: 1133.6 bits (2931), Expect = 0.0e+00
Identity = 543/578 (93.94%), Postives = 557/578 (96.37%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MA SITHQIGALAGTPV SE SN ++GEA AAVN   LRKSPAS LRCRVQRTDGVDALS
Sbjct: 1   MAFSITHQIGALAGTPVTSEMSNGASGEATAAVNAAVLRKSPASTLRCRVQRTDGVDALS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGGIRPDLSVACQAFATEV APTEV+EYKEGGERGKGKGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGIRPDLSVACQAFATEVAAPTEVKEYKEGGERGKGKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMSFHQCGGNVGDSVTIPLPKW VEEIDKDPDLAYTDQWGRRNYEYISLGCDNL
Sbjct: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWVVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAF++NFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTWKFPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFKENFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWKFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEA GKPEWG TGPTDAG YNNWPEDTPFFRK+GGGWNSTYG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEAFGKPEWGVTGPTDAGSYNNWPEDTPFFRKDGGGWNSTYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFLSWYSQMLL HG+RILTAATSTF+NTG+KISVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLSWYSQMLLAHGERILTAATSTFENTGVKISVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG
Sbjct: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDE AH QILQASSF G+G DSEE EMCAFTYLRMNPHLFEA+NWRRFVAFVKK
Sbjct: 481 ENALPRYDESAHGQILQASSFIGEG-DSEEAEMCAFTYLRMNPHLFEAENWRRFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNPDKCWEQ+EREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPDKCWEQLEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. ExPASy TrEMBL
Match: A0A0A0L480 (Beta-amylase OS=Cucumis sativus OX=3659 GN=Csa_3G133950 PE=3 SV=1)

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 541/578 (93.60%), Postives = 559/578 (96.71%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MALSITHQIGALAGTPV SEASN SAGEA  AVNT  LRKS AS LRCRVQRTDGVDALS
Sbjct: 1   MALSITHQIGALAGTPVTSEASNISAGEASLAVNTAMLRKSSASPLRCRVQRTDGVDALS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKE GE+GK KGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEEGEKGKEKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTM NTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMENTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEE+DKDPDLAYTDQWGRRNYEYISLGCD L
Sbjct: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEMDKDPDLAYTDQWGRRNYEYISLGCDTL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAF+DNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTWKFPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFKDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWKFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEA+GKPEWGSTGPTDAGGY++WPED PFFRKEGGGWNSTYG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEASGKPEWGSTGPTDAGGYHSWPEDNPFFRKEGGGWNSTYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFL+WYSQMLLDHGDRILTAATS F+ TG+KISVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLTWYSQMLLDHGDRILTAATSIFEKTGVKISVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           R+RDGYLPIA+MLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVA ATHKAQVPLAG
Sbjct: 421 RYRDGYLPIARMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVAQATHKAQVPLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDEFAH+QILQASSF GD +DS+E+EMCAFTYLRMNPHLFEA+NWRRFVAFVKK
Sbjct: 481 ENALPRYDEFAHEQILQASSFVGD-EDSKESEMCAFTYLRMNPHLFEAENWRRFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNPDKCWEQVEREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. ExPASy TrEMBL
Match: A0A6J1DY10 (Beta-amylase OS=Momordica charantia OX=3673 GN=LOC111024550 PE=3 SV=1)

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 532/578 (92.04%), Postives = 553/578 (95.67%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MALSITHQIGALAGTPV SE SNNS+GEA AA+N   LRKSPAS LRCRVQRTD VD LS
Sbjct: 1   MALSITHQIGALAGTPVTSETSNNSSGEAAAALNAAVLRKSPASTLRCRVQRTDVVDTLS 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATEVEAPTEVREYKEGGERGKGKGVPVYVMMPLDS 120
           PP+SPCRSPVLGG+RPDLSVACQAFATEVEAP EVREYKEG E+ KGKGVPVYVMMPLDS
Sbjct: 61  PPLSPCRSPVLGGMRPDLSVACQAFATEVEAPAEVREYKEGREKEKGKGVPVYVMMPLDS 120

Query: 121 VTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLEMAKKH 180
           VTMGNTVNRRKAMNASLQALKSAGVEG+MMDVWWGLVERD+PGSYNWGGYTELLEMAKKH
Sbjct: 121 VTMGNTVNRRKAMNASLQALKSAGVEGVMMDVWWGLVERDAPGSYNWGGYTELLEMAKKH 180

Query: 181 GLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISLGCDNL 240
           GLKVQAVMS+HQCGGNVGDSVTIPLPKW VEEIDKDPDLAYTDQWG RNYEY+SLGCDNL
Sbjct: 181 GLKVQAVMSYHQCGGNVGDSVTIPLPKWVVEEIDKDPDLAYTDQWGGRNYEYLSLGCDNL 240

Query: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGTWKFPG 300
           PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQ+GTW+FPG
Sbjct: 241 PVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQNGTWRFPG 300

Query: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGWNSTYG 360
           IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAG YNNWPEDT FFRKEGGGWN +YG
Sbjct: 301 IGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGSYNNWPEDTTFFRKEGGGWNCSYG 360

Query: 361 EFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTAGYYNT 420
           EFFLSWYSQMLLDHGDRILTAA STF+NTG+KISVKIAGIHWHYGHRSHAPELTAGYYNT
Sbjct: 361 EFFLSWYSQMLLDHGDRILTAAKSTFENTGVKISVKIAGIHWHYGHRSHAPELTAGYYNT 420

Query: 421 RFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQVPLAG 480
           RFRDGYLPIAQMLARHGAIFNFTC+EMRDHEQPQDALCAPEKLVRQVA+ATHKA V LAG
Sbjct: 421 RFRDGYLPIAQMLARHGAIFNFTCLEMRDHEQPQDALCAPEKLVRQVAMATHKAGVRLAG 480

Query: 481 ENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEADNWRRFVAFVKK 540
           ENALPRYDEFAH+QILQASSF G+G DSEE EMCAFTYLRMNP LF+ADNWR+FVAFVKK
Sbjct: 481 ENALPRYDEFAHEQILQASSFKGEG-DSEEREMCAFTYLRMNPQLFQADNWRKFVAFVKK 540

Query: 541 MKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           MKEGKNP+KCWEQVEREAEHFVHVTQPLVQ AAVALMH
Sbjct: 541 MKEGKNPEKCWEQVEREAEHFVHVTQPLVQEAAVALMH 577

[Close]

BLAST of Spg031720 vs. TAIR 10
Match: AT3G23920.1 (beta-amylase 1 )

HSP 1 Score: 913.3 bits (2359), Expect = 9.9e-266
Identity = 448/584 (76.71%), Postives = 500/584 (85.62%), Query Frame = 0


Query: 1   MALSITHQIGALAGTPVVSEASNNSAGEAMAAVNTTALRKSPASALRCRVQRTDGVDALS 60
           MAL+++HQ+G LAGTP+ S    +S   ++ +++  + R     A+  R  +  G D  S
Sbjct: 1   MALNLSHQLGVLAGTPIKSGEMTDS---SLLSISPPSARMMTPKAMN-RNYKAHGTDP-S 60

Query: 61  PPVSPCRSPVLGGIRPDLSVACQAFATE--VEAPTEVREYKEGGERGK---GKGVPVYVM 120
           PP+    SP+LG  R DLSVAC+AFA E  +    E R Y+EGG  GK   G GVPV+VM
Sbjct: 61  PPM----SPILGATRADLSVACKAFAVENGIGTIEEQRTYREGGIGGKKEGGGGVPVFVM 120

Query: 121 MPLDSVTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGGYTELLE 180
           MPLDSVTMGNTVNRRKAM ASLQALKSAGVEGIM+DVWWGLVE++SPG+YNWGGY ELLE
Sbjct: 121 MPLDSVTMGNTVNRRKAMKASLQALKSAGVEGIMIDVWWGLVEKESPGTYNWGGYNELLE 180

Query: 181 MAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRNYEYISL 240
           +AKK GLKVQAVMSFHQCGGNVGDSVTIPLP+W VEE+DKDPDLAYTDQWGRRN+EYISL
Sbjct: 181 LAKKLGLKVQAVMSFHQCGGNVGDSVTIPLPQWVVEEVDKDPDLAYTDQWGRRNHEYISL 240

Query: 241 GCDNLPVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSYPEQDGT 300
           G D LPVLKGRTPVQCYADFMRAFRDNFKHLLG+TIVEIQVGMGPAGELRYPSYPEQ+GT
Sbjct: 241 GADTLPVLKGRTPVQCYADFMRAFRDNFKHLLGETIVEIQVGMGPAGELRYPSYPEQEGT 300

Query: 301 WKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFRKEGGGW 360
           WKFPGIGAFQC+DKY LSSLKAAAE  GKPEWGSTGPTDAG YNNWPEDT FF+KEGGGW
Sbjct: 301 WKFPGIGAFQCYDKYSLSSLKAAAETYGKPEWGSTGPTDAGHYNNWPEDTQFFKKEGGGW 360

Query: 361 NSTYGEFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSHAPELTA 420
           NS YG+FFLSWYSQMLLDHG+RIL++A S F+N G+KISVKIAGIHWHYG RSHAPELTA
Sbjct: 361 NSEYGDFFLSWYSQMLLDHGERILSSAKSIFENMGVKISVKIAGIHWHYGTRSHAPELTA 420

Query: 421 GYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVALATHKAQ 480
           GYYNTRFRDGYLPIAQMLARH AIFNFTCIEMRDHEQPQDALCAPEKLV QVALAT  A+
Sbjct: 421 GYYNTRFRDGYLPIAQMLARHNAIFNFTCIEMRDHEQPQDALCAPEKLVNQVALATLAAE 480

Query: 481 VPLAGENALPRYDEFAHDQILQASSFNGD-GDDSEETEMCAFTYLRMNPHLFEADNWRRF 540
           VPLAGENALPRYD++AH+QIL+AS+ N D  ++ E  EMCAFTYLRMNP LF+ADNW +F
Sbjct: 481 VPLAGENALPRYDDYAHEQILKASALNLDQNNEGEPREMCAFTYLRMNPELFQADNWGKF 540

Query: 541 VAFVKKMKEGKNPDKCWEQVEREAEHFVHVTQPLVQAAAVALMH 579
           VAFVKKM EG++  +C E+VEREAEHFVHVTQPLVQ AAVAL H
Sbjct: 541 VAFVKKMGEGRDSHRCREEVEREAEHFVHVTQPLVQEAAVALTH 575

[Close]

BLAST of Spg031720 vs. TAIR 10
Match: AT4G17090.1 (chloroplast beta-amylase )

HSP 1 Score: 603.2 bits (1554), Expect = 2.2e-172
Identity = 277/436 (63.53%), Postives = 341/436 (78.21%), Query Frame = 0


Query: 110 VPVYVMMPLDSVTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGG 169
           VPV+VM+PLD+VTM   +N+ +AMNASL ALK AGVEG+M+D WWGLVE+D P +YNW G
Sbjct: 86  VPVFVMLPLDTVTMSGHLNKPRAMNASLMALKGAGVEGVMVDAWWGLVEKDGPMNYNWEG 145

Query: 170 YTELLEMAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRN 229
           Y EL++M +KHGLK+Q VMSFHQCGGNVGDS +IPLP W +EEI K+PDL YTD+ GRRN
Sbjct: 146 YAELIQMVQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDKSGRRN 205

Query: 230 YEYISLGCDNLPVLKGRTPVQCYADFMRAFRDNFKHLLGDTIVEIQVGMGPAGELRYPSY 289
            EYISLGCD++PVL+GRTP+Q Y+DFMR+FR+ F+  +G  I EIQVGMGP GELRYPSY
Sbjct: 206 PEYISLGCDSVPVLRGRTPIQVYSDFMRSFRERFEGYIGGVIAEIQVGMGPCGELRYPSY 265

Query: 290 PEQDGTWKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFFR 349
           PE +GTW+FPGIG FQC+DKYM SSL+A AE+ GK  WG++GP DAG Y N PEDT FFR
Sbjct: 266 PESNGTWRFPGIGEFQCYDKYMKSSLQAYAESIGKTNWGTSGPHDAGEYKNLPEDTEFFR 325

Query: 350 KEGGGWNSTYGEFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRSH 409
           ++ G WNS YG+FF+ WYS  LL+HGD++L++A   F+ +G K+S K+AGIHWHY  RSH
Sbjct: 326 RD-GTWNSEYGKFFMEWYSGKLLEHGDQLLSSAKGIFQGSGAKLSGKVAGIHWHYNTRSH 385

Query: 410 APELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVAL 469
           A ELTAGYYNTR  DGYLPIA+M  +HG + NFTC+EM+D EQP+ A C+PE LV+QV  
Sbjct: 386 AAELTAGYYNTRNHDGYLPIAKMFNKHGVVLNFTCMEMKDGEQPEHANCSPEGLVKQVQN 445

Query: 470 ATHKAQVPLAGENALPRYDEFAHDQILQAS-SFNGDGDDSEETEMCAFTYLRMNPHLFEA 529
           AT +A   LAGENAL RYD  A  Q++  + S +G+G       + AFTYLRMN  LFE 
Sbjct: 446 ATRQAGTELAGENALERYDSSAFGQVVATNRSDSGNG-------LTAFTYLRMNKRLFEG 505

Query: 530 DNWRRFVAFVKKMKEG 545
            NW++ V FVK MKEG
Sbjct: 506 QNWQQLVEFVKNMKEG 513

[Close]

BLAST of Spg031720 vs. TAIR 10
Match: AT4G15210.1 (beta-amylase 5 )

HSP 1 Score: 443.7 bits (1140), Expect = 2.2e-124
Identity = 213/435 (48.97%), Postives = 292/435 (67.13%), Query Frame = 0


Query: 110 VPVYVMMPLDSVTMGNTVNRRKAMNASLQALK-SAGVEGIMMDVWWGLVERDSPGSYNWG 169
           VPVYVM+PL  V + N     + +   L+ LK  AGV+G+M+DVWWG++E   P  Y+W 
Sbjct: 14  VPVYVMLPLGVVNVENVFADPETLETQLKRLKEEAGVDGVMVDVWWGIIESKGPKQYDWT 73

Query: 170 GYTELLEMAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRR 229
            Y  L ++  + GLK+QA+MSFHQCGGNVGD VTIP+P+W  +  D DPD+ YT++ G R
Sbjct: 74  AYKTLFQLIARLGLKIQAIMSFHQCGGNVGDIVTIPIPQWVRDVGDNDPDIYYTNRKGTR 133

Query: 230 NYEYISLGCDNLPVLKGRTPVQCYADFMRAFRDNFKHLL-GDTIVEIQVGMGPAGELRYP 289
           + EY+S+G DNLP+  GRT VQ Y+D+M +F++N   L+    IV+I+VG+GPAGELRYP
Sbjct: 134 DIEYLSIGVDNLPLFAGRTAVQLYSDYMSSFKENMADLIEAGVIVDIEVGLGPAGELRYP 193

Query: 290 SYPEQDGTWKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPF 349
           SYP+  G W FPGIG FQC+DKY+    K AA  AG PEW    P DAG YN+ PE+T F
Sbjct: 194 SYPQSQG-WVFPGIGEFQCYDKYLKKDFKEAAAKAGHPEWDL--PEDAGEYNDKPEETGF 253

Query: 350 FRKEGGGWNSTYGEFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHR 409
           F+K+ G + S  G+FF++WYS  L+ HGD+IL  A   F    + ++ K++GIHW Y H 
Sbjct: 254 FKKD-GTYVSEKGKFFMTWYSNKLIFHGDQILGEANKIFAGLKVNLAAKVSGIHWLYNHH 313

Query: 410 SHAPELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQV 469
           SHA ELTAGYYN   RDGY PIA+ML++H  I NFTC+EM+D +   +AL AP++LV++V
Sbjct: 314 SHAAELTAGYYNLFKRDGYRPIARMLSKHYGILNFTCLEMKDTDNTAEALSAPQELVQEV 373

Query: 470 ALATHKAQVPLAGENALPRYDEFAHDQILQASSFNG-DGDDSEETEMCAFTYLRMNPHLF 529
                K  + +AGENAL  Y    ++QIL  +  NG + +   +  M  FTYLR++  +F
Sbjct: 374 LSKAWKEGIEVAGENALETYGAKGYNQILLNARPNGVNPNGKPKLRMYGFTYLRLSDTVF 433

Query: 530 EADNWRRFVAFVKKM 542
           + +N+  F   V+KM
Sbjct: 434 QENNFELFKKLVRKM 444

[Close]

BLAST of Spg031720 vs. TAIR 10
Match: AT4G00490.1 (beta-amylase 2 )

HSP 1 Score: 436.0 bits (1120), Expect = 4.6e-122
Identity = 218/443 (49.21%), Postives = 290/443 (65.46%), Query Frame = 0


Query: 110 VPVYVMMPLDSVTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGG 169
           VPVYVM+PL  + M + V   + +   L+ LKS  V+G+M+D WWG+VE  +P  YNW G
Sbjct: 95  VPVYVMLPLGVIDMNSEVVEPEELLDQLRTLKSVNVDGVMVDCWWGIVESHTPQVYNWSG 154

Query: 170 YTELLEMAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRN 229
           Y +L +M ++ GLK+Q VMSFH+CGGNVGD V I +P+W  E    +PD+ +TD  GRRN
Sbjct: 155 YKKLFQMIRELGLKIQVVMSFHECGGNVGDDVHIQIPEWVREIGQSNPDIYFTDSAGRRN 214

Query: 230 YEYISLGCDNLPVLKGRTPVQCYADFMRAFRDNFKHLLGDTIV-EIQVGMGPAGELRYPS 289
            E ++ G D   VL+GRT ++ Y D+MR+FR  F     + I+ EI+VG+GP GELRYPS
Sbjct: 215 TECLTWGIDKQRVLRGRTALEVYFDYMRSFRVEFDEFFEEKIIPEIEVGLGPCGELRYPS 274

Query: 290 YPEQDGTWKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFF 349
           YP Q G WK+PGIG FQC+DKY+++SLK AAE  G   WG  GP +   YN+ P  T FF
Sbjct: 275 YPAQFG-WKYPGIGEFQCYDKYLMNSLKEAAEVRGHSFWG-RGPDNTETYNSTPHGTGFF 334

Query: 350 RKEGGGWNSTYGEFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRS 409
           R +GG ++S YG FFL+WYS++L+DHGDR+L  A   F+  G  I+ K++GIHW Y   S
Sbjct: 335 R-DGGDYDSYYGRFFLNWYSRVLIDHGDRVLAMANLAFE--GTCIAAKLSGIHWWYKTAS 394

Query: 410 HAPELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMR---DHEQPQDALCAPEKLVR 469
           HA ELTAG+YN+  RDGY PIA M  +H A  NFTC+E+R    HE   +AL  PE LV 
Sbjct: 395 HAAELTAGFYNSSNRDGYGPIAAMFKKHDAALNFTCVELRTLDQHEDFPEALADPEGLVW 454

Query: 470 QVALATHKAQVPLAGENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHL 529
           QV  A   A +P+A ENALP YD   +++IL+ +    D D      +  FTYLR+NP L
Sbjct: 455 QVLNAAWDASIPVASENALPCYDREGYNKILENAKPLTDPDG---RHLSCFTYLRLNPTL 514

Query: 530 FEADNWRRFVAFVKKMKEGKNPD 549
            E+ N++ F  F+K+M     PD
Sbjct: 515 MESQNFKEFERFLKRMHGEAVPD 529

[Close]

BLAST of Spg031720 vs. TAIR 10
Match: AT2G32290.1 (beta-amylase 6 )

HSP 1 Score: 432.2 bits (1110), Expect = 6.7e-121
Identity = 211/460 (45.87%), Postives = 289/460 (62.83%), Query Frame = 0


Query: 110 VPVYVMMPLDSVTMGNTVNRRKAMNASLQALKSAGVEGIMMDVWWGLVERDSPGSYNWGG 169
           VPVYVM+ L  +T  N +   +++   L+ LK + V+G+M+DVWWG+VE   P  Y W  
Sbjct: 78  VPVYVMLQLGVITNDNVLENEESLKKQLKKLKQSQVDGVMVDVWWGIVESKGPKQYQWSA 137

Query: 170 YTELLEMAKKHGLKVQAVMSFHQCGGNVGDSVTIPLPKWAVEEIDKDPDLAYTDQWGRRN 229
           Y  L  + +  GLK+QA+MSFH+CGGN+GD V IP+PKW +E  D +PD+ YT++ G RN
Sbjct: 138 YRNLFAIVQSFGLKLQAIMSFHRCGGNIGDDVNIPIPKWVLEIGDSNPDIFYTNKSGNRN 197

Query: 230 YEYISLGCDNLPVLKGRTPVQCYADFMRAFRDNFKHLLGD-TIVEIQVGMGPAGELRYPS 289
            E +SL  DNL + +GRT V+ Y D+M++FR+N +  +    I++I+VG+GPAGELRYPS
Sbjct: 198 KECLSLSVDNLSLFRGRTAVEMYRDYMKSFRENMEDFISSGVIIDIEVGLGPAGELRYPS 257

Query: 290 YPEQDGTWKFPGIGAFQCFDKYMLSSLKAAAEAAGKPEWGSTGPTDAGGYNNWPEDTPFF 349
           Y E  G W FPGIG FQC+DKY+ S  +      G PEW    P +AG YN+ P +T FF
Sbjct: 258 YSETQG-WVFPGIGEFQCYDKYLRSDYEEEVRRIGHPEWKL--PENAGEYNSVPGETEFF 317

Query: 350 RKEGGGWNSTYGEFFLSWYSQMLLDHGDRILTAATSTFKNTGIKISVKIAGIHWHYGHRS 409
               G +    G FFLSWYS+ LL HGD+IL  A   F    +KI+ K++GIHW Y   S
Sbjct: 318 EYSNGTYLKEEGNFFLSWYSKKLLLHGDQILDEANKVFLGCKLKIAAKVSGIHWWYKTES 377

Query: 410 HAPELTAGYYNTRFRDGYLPIAQMLARHGAIFNFTCIEMRDHEQPQDALCAPEKLVRQVA 469
           HA ELTAGYYN + RDGY  IA+++ RH AI NFTC+EM++ EQP  A   P++LV+QV 
Sbjct: 378 HAAELTAGYYNLKNRDGYRAIAKIMRRHHAILNFTCLEMKNTEQPAKAKSGPQELVQQVL 437

Query: 470 LATHKAQVPLAGENALPRYDEFAHDQILQASSFNGDGDDSEETEMCAFTYLRMNPHLFEA 529
            +  +  + +AGENALPR+D   ++QI+  +  NG   D  +  M  FTYLR++  L   
Sbjct: 438 SSGWREGIEVAGENALPRFDRNGYNQIILNARPNGVNQDG-KPRMFGFTYLRLSDKLLNE 497

Query: 530 DNWRRFVAFVKKMKEGKNPDKCWEQVEREAEHFVHVTQPL 569
            N+  F  F+K+M    N + C      E E + H   PL
Sbjct: 498 PNFSTFKMFLKRMH--ANQEYC-----SEPERYNHELLPL 526

The following BLAST results are available for this feature:

BLAST of Spg031720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008438436.1	0.0e+00	93.94	PREDICTED: beta-amylase 1, chloroplastic-like [Cucumis melo]	[more]
XP_022980262.1	0.0e+00	94.12	beta-amylase 1, chloroplastic-like [Cucurbita maxima]	[more]
KAG6582430.1	0.0e+00	93.94	Beta-amylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022924792.1	0.0e+00	93.94	beta-amylase 1, chloroplastic-like [Cucurbita moschata]	[more]
KAG7018828.1	0.0e+00	93.60	Beta-amylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Spg031720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LIR6	1.4e-264	76.71	Beta-amylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM1 PE=1 SV=1	[more]
Q10RZ1	1.2e-212	65.05	Beta-amylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY2 ...	[more]
Q9AV88	2.8e-204	66.11	Beta-amylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY1 ...	[more]
O23553	3.1e-171	63.53	Beta-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM3 PE=1 SV=3	[more]
P82993	7.2e-128	51.60	Beta-amylase OS=Hordeum vulgare subsp. spontaneum OX=77009 GN=BMY1 PE=1 SV=1	[more]

BLAST of Spg031720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AWG6	0.0e+00	93.94	Beta-amylase OS=Cucumis melo OX=3656 GN=LOC103483532 PE=3 SV=1	[more]
A0A6J1IQX2	0.0e+00	94.12	Beta-amylase OS=Cucurbita maxima OX=3661 GN=LOC111479682 PE=3 SV=1	[more]
A0A6J1E9Z7	0.0e+00	93.94	Beta-amylase OS=Cucurbita moschata OX=3662 GN=LOC111432184 PE=3 SV=1	[more]
A0A0A0L480	0.0e+00	93.60	Beta-amylase OS=Cucumis sativus OX=3659 GN=Csa_3G133950 PE=3 SV=1	[more]
A0A6J1DY10	0.0e+00	92.04	Beta-amylase OS=Momordica charantia OX=3673 GN=LOC111024550 PE=3 SV=1	[more]

BLAST of Spg031720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23920.1	9.9e-266	76.71	beta-amylase 1	[more]
AT4G17090.1	2.2e-172	63.53	chloroplast beta-amylase	[more]
AT4G15210.1	2.2e-124	48.97	beta-amylase 5	[more]
AT4G00490.1	4.6e-122	49.21	beta-amylase 2	[more]
AT2G32290.1	6.7e-121	45.87	beta-amylase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001554	Glycoside hydrolase, family 14	PRINTS	PR00750	BETAAMYLASE	coord: 355..374 score: 50.0 coord: 144..158 score: 69.0 coord: 279..301 score: 55.22 coord: 408..419 score: 77.5 coord: 165..183 score: 37.89 coord: 391..407 score: 41.47 coord: 463..485 score: 45.87 coord: 187..208 score: 75.45 coord: 426..449 score: 57.92
IPR001554	Glycoside hydrolase, family 14	PFAM	PF01373	Glyco_hydro_14	coord: 113..537 e-value: 1.7E-137 score: 458.6
IPR001554	Glycoside hydrolase, family 14	PANTHER	PTHR31352	FAMILY NOT NAMED	coord: 37..576
IPR001371	Glycoside hydrolase, family 14B, plant	PRINTS	PR00842	GLHYDLASE14B	coord: 269..278 score: 46.92 coord: 485..494 score: 46.15 coord: 429..439 score: 62.94 coord: 532..546 score: 32.31 coord: 517..531 score: 43.59
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 102..575 e-value: 9.4E-217 score: 722.2
None	No IPR available	PANTHER	PTHR31352:SF38	BETA-AMYLASE 1, CHLOROPLASTIC	coord: 37..576
IPR018238	Glycoside hydrolase, family 14, conserved site	PROSITE	PS00506	BETA_AMYLASE_1	coord: 191..199
IPR018238	Glycoside hydrolase, family 14, conserved site	PROSITE	PS00679	BETA_AMYLASE_2	coord: 279..289
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 108..551

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031720.1	Spg031720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000272	polysaccharide catabolic process
molecular_function	GO:0102229	amylopectin maltohydrolase activity
molecular_function	GO:0016161	beta-amylase activity