Spg031701 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031701
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Protease inhibitor protein
Location	scaffold11: 40088873 .. 40089704 (-)
RNA-Seq Expression	Spg031701
Synteny	Spg031701

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGAAGAATCTGCTCCTCGTAAGTTCATCCTATCAATACTCCATTTTGAAAAACTTGGTTCCATGGAATGATTTGTGCATGCACACAACCATAACATTATTGCATTTTTGGAAAAATCATTGAATAGAAAAATCATTGAGACGAAAGAAGAGAAAAACGCAGAGGGATTCCAAGAGAAAAATCTCTATCACATTGAATTGAATTCAACTATTGAAGAGGCAGCTACATATATACTGCTAGATAGAGATGAAAAAACTCTTGGAAAAATAATAACAAACCCTAACCCTAGATCACGAAATCTTACCTCTGAGAGCTATCACGAAAATTACATCAGAGAGAGTGAGAGTTCTAGAAGACAACTATTCTAGAACCTTTACATATAACCCTATCAATCTGATAAAGAGAAAATGGGTTGGGCTTCATAATATGGGCTTATGCCTTTTACAAAGAGTATGAGCTGGACAAATAATAAAATTTCAGTGGGCTGCCTTTACGTTAACATCTTCGTCGTAGTTTTTTTTTTTTTTTTATGTAATTAAGATTTATTATTATAAATACCTTTTGAGTGTATTTGATTGGATTATATATAGGTGATATTTATTGCCAAATTTAACACAAATGTATTTCTAAATAGGGAAAAGTCAATGGCCAGAGCTTGTCTTTGTAAAATTCTCTATTGCTGCTGCGATCATAGAAAAAGAGAATCCTGATGTCACAGCTTTCAAGATCCTATCTGGGAGTAAAAGAATTATGGATTTTGATCGTACACGAGTTTTTGTTGATGTCAATATTGAAGATGTGGTTGTTAAAGTTCCTAAGGTCGGCTAG

mRNA sequence

ATGTCTGAAGAATCTGCTCCTCGGAAAAGTCAATGGCCAGAGCTTGTCTTTGTAAAATTCTCTATTGCTGCTGCGATCATAGAAAAAGAGAATCCTGATGTCACAGCTTTCAAGATCCTATCTGGGAGTAAAAGAATTATGGATTTTGATCGTACACGAGTTTTTGTTGATGTCAATATTGAAGATGTGGTTGTTAAAGTTCCTAAGGTCGGCTAG

Coding sequence (CDS)

ATGTCTGAAGAATCTGCTCCTCGGAAAAGTCAATGGCCAGAGCTTGTCTTTGTAAAATTCTCTATTGCTGCTGCGATCATAGAAAAAGAGAATCCTGATGTCACAGCTTTCAAGATCCTATCTGGGAGTAAAAGAATTATGGATTTTGATCGTACACGAGTTTTTGTTGATGTCAATATTGAAGATGTGGTTGTTAAAGTTCCTAAGGTCGGCTAG

Protein sequence

MSEESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNIEDVVVKVPKVG

Homology

[Close]

BLAST of Spg031701 vs. NCBI nr
Match: KGN56897.1 (hypothetical protein Csa_010510 [Cucumis sativus])

HSP 1 Score: 111.7 bits (278), Expect = 2.6e-21
Identity = 54/71 (76.06%), Postives = 65/71 (91.55%), Query Frame = 0


Query: 1  MSEESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNI 60
          M+EESAPRK++WPELVFVK+SIAAAIIEKENPDV A KILSG+ RI++FD  RV+VD+N+
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 EDVVVKVPKVG 72
          E+VVVK PKVG
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Spg031701 vs. NCBI nr
Match: KAA0049288.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 105.1 bits (261), Expect = 2.5e-19
Identity = 49/68 (72.06%), Postives = 62/68 (91.18%), Query Frame = 0


Query: 3  EESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNIED 62
          ++SAPRK+QWPELVF+K+SIAAAIIE+ENPDV A KILSG+ RI++FD  RV+ DVN+ED
Sbjct: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63

Query: 63 VVVKVPKV 71
          V+VK+PKV
Sbjct: 64 VIVKMPKV 71

[Close]

BLAST of Spg031701 vs. NCBI nr
Match: KAA0049287.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 105.1 bits (261), Expect = 2.5e-19
Identity = 52/69 (75.36%), Postives = 59/69 (85.51%), Query Frame = 0


Query: 3  EESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNIED 62
          ++SAPRK+QWPELVFVK+S AAAIIEKENPDV A KILSG  RI +FD  RV+ DVN+ED
Sbjct: 4  DQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVED 63

Query: 63 VVVKVPKVG 72
          VVVK PKVG
Sbjct: 64 VVVKTPKVG 72

[Close]

BLAST of Spg031701 vs. NCBI nr
Match: KAA0049286.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 99.0 bits (245), Expect = 1.8e-17
Identity = 50/71 (70.42%), Postives = 59/71 (83.10%), Query Frame = 0


Query: 1  MSEESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNI 60
          M+EESA RK++WPELVFV+FS AA IIEKENPDV A KIL+ S RI +FD +RV+VD N+
Sbjct: 1  MAEESAQRKTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNV 60

Query: 61 EDVVVKVPKVG 72
          ED VVKVP VG
Sbjct: 61 EDKVVKVPSVG 71

[Close]

BLAST of Spg031701 vs. NCBI nr
Match: KAE8650337.1 (hypothetical protein Csa_010581 [Cucumis sativus])

HSP 1 Score: 90.1 bits (222), Expect = 8.2e-15
Identity = 47/68 (69.12%), Postives = 54/68 (79.41%), Query Frame = 0


Query: 1  MSEESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNI 60
          M+EESA RK++WPELVFV F  AA IIEKENPDV A KIL  S RI +FD +RV+VD NI
Sbjct: 1  MAEESAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNI 60

Query: 61 EDVVVKVP 69
          E+ VVKVP
Sbjct: 61 EERVVKVP 68

[Close]

BLAST of Spg031701 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 8.5e-07
Identity = 30/67 (44.78%), Postives = 40/67 (59.70%), Query Frame = 0


Query: 5  SAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNIEDVV 64
          S P KS WP LV V  S+A AIIE++NP+V A  +  G+    DF   RV + VN   +V
Sbjct: 2  SCPGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLV 61

Query: 65 VKVPKVG 72
          V  P++G
Sbjct: 62 VSPPRIG 68

[Close]

BLAST of Spg031701 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 1.6e-05
Identity = 31/67 (46.27%), Postives = 38/67 (56.72%), Query Frame = 0


Query: 7  PRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMD--FDRTRVFVDVNIEDVV 66
          P K+ WPELV     IAA II+ EN +V A  +  G     D  F+R RVFVD N   VV
Sbjct: 6  PVKNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDEN--RVV 65

Query: 67 VKVPKVG 72
           +VP +G
Sbjct: 66 TQVPAIG 70

[Close]

BLAST of Spg031701 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.6e-05
Identity = 28/64 (43.75%), Postives = 37/64 (57.81%), Query Frame = 0


Query: 7  PRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNIEDVVVK 66
          P K+ WPELV    ++AAA +E+EN +V A  +  GS    DF   RV+V VN   VV  
Sbjct: 5  PGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTS 64

Query: 67 VPKV 71
          VP +
Sbjct: 65 VPHI 68

[Close]

BLAST of Spg031701 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.6e-05
Identity = 30/73 (41.10%), Postives = 43/73 (58.90%), Query Frame = 0


Query: 2  SEESAP--RKSQWPELVFVKFSIAAAIIEKENPDVTAF-KILSGSKRIMDFDRTRVFVDV 61
          ++ S P  +K  WPEL+ V    A  II+KEN  +T    +L+GS    DF   RV + V
Sbjct: 22 AQSSCPGNKKETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFV 81

Query: 62 NIEDVVVKVPKVG 72
          N+ D VV++P+VG
Sbjct: 82 NVLDFVVQIPRVG 94

[Close]

BLAST of Spg031701 vs. ExPASy Swiss-Prot
Match: P20076 (Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 3.6e-05
Identity = 28/64 (43.75%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 9   KSQWPELVFVKFSIAAAIIEKENPDVTAFK-ILSGSKRIMDFDRTRVFVDVNIEDVVVKV 68
           K  WPEL+      A  II+KENP +T  + +L+GS    D    RV + VN+ D+VV+ 
Sbjct: 56  KESWPELLGTPAKFAKQIIQKENPKLTNVETLLNGSAFTEDLRCNRVRLFVNLLDIVVQT 115

Query: 69  PKVG 72
           PKVG
Sbjct: 116 PKVG 119

[Close]

BLAST of Spg031701 vs. ExPASy TrEMBL
Match: A0A0A0L8E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.3e-21
Identity = 54/71 (76.06%), Postives = 65/71 (91.55%), Query Frame = 0


Query: 1  MSEESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNI 60
          M+EESAPRK++WPELVFVK+SIAAAIIEKENPDV A KILSG+ RI++FD  RV+VD+N+
Sbjct: 1  MAEESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINV 60

Query: 61 EDVVVKVPKVG 72
          E+VVVK PKVG
Sbjct: 61 EEVVVKKPKVG 71

[Close]

BLAST of Spg031701 vs. ExPASy TrEMBL
Match: A0A5A7U228 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001230 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.2e-19
Identity = 49/68 (72.06%), Postives = 62/68 (91.18%), Query Frame = 0


Query: 3  EESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNIED 62
          ++SAPRK+QWPELVF+K+SIAAAIIE+ENPDV A KILSG+ RI++FD  RV+ DVN+ED
Sbjct: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63

Query: 63 VVVKVPKV 71
          V+VK+PKV
Sbjct: 64 VIVKMPKV 71

[Close]

BLAST of Spg031701 vs. ExPASy TrEMBL
Match: A0A5A7U4L9 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001240 PE=3 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.2e-19
Identity = 52/69 (75.36%), Postives = 59/69 (85.51%), Query Frame = 0


Query: 3  EESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNIED 62
          ++SAPRK+QWPELVFVK+S AAAIIEKENPDV A KILSG  RI +FD  RV+ DVN+ED
Sbjct: 4  DQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVNVED 63

Query: 63 VVVKVPKVG 72
          VVVK PKVG
Sbjct: 64 VVVKTPKVG 72

[Close]

BLAST of Spg031701 vs. ExPASy TrEMBL
Match: A0A5A7U4U0 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001250 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 8.5e-18
Identity = 50/71 (70.42%), Postives = 59/71 (83.10%), Query Frame = 0


Query: 1  MSEESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNI 60
          M+EESA RK++WPELVFV+FS AA IIEKENPDV A KIL+ S RI +FD +RV+VD N+
Sbjct: 1  MAEESAQRKTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNV 60

Query: 61 EDVVVKVPKVG 72
          ED VVKVP VG
Sbjct: 61 EDKVVKVPSVG 71

[Close]

BLAST of Spg031701 vs. ExPASy TrEMBL
Match: A0A0A0L785 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.1e-16
Identity = 49/71 (69.01%), Postives = 56/71 (78.87%), Query Frame = 0


Query: 1  MSEESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNI 60
          M+EESA RK++WPELVFV F  AA IIEKENPDV A KIL  S RI +FD +RV+VD NI
Sbjct: 1  MAEESAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNI 60

Query: 61 EDVVVKVPKVG 72
          E+ VVKVP VG
Sbjct: 61 EERVVKVPSVG 71

[Close]

BLAST of Spg031701 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 60.8 bits (146), Expect = 5.0e-10
Identity = 39/73 (53.42%), Postives = 43/73 (58.90%), Query Frame = 0


Query: 1  MSEESAPRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDF--DRTRVFVDV 60
          MS E  PRK+ WPEL       AA +IE+ENP V A  IL GS    DF  DR RVFVD 
Sbjct: 1  MSTE-CPRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDG 60

Query: 61 NIEDVVVKVPKVG 72
          N   +VVK PK G
Sbjct: 61 N--RIVVKTPKSG 70

[Close]

BLAST of Spg031701 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 48.1 bits (113), Expect = 3.3e-06
Identity = 28/65 (43.08%), Postives = 39/65 (60.00%), Query Frame = 0


Query: 7  PRKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNIEDVVVK 66
          P KS WPEL+  K   A  +IE+ENP + A  IL G+     F  +RV+V VN   +VV+
Sbjct: 35 PGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVNDCGIVVQ 94

Query: 67 VPKVG 72
          +P +G
Sbjct: 95 IPIIG 99

[Close]

BLAST of Spg031701 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 47.4 bits (111), Expect = 5.7e-06
Identity = 26/64 (40.62%), Postives = 35/64 (54.69%), Query Frame = 0


Query: 8  RKSQWPELVFVKFSIAAAIIEKENPDVTAFKILSGSKRIMDFDRTRVFVDVNIEDVVVKV 67
          +K +WPELV     +A   IE+ENP+V A  +  G KRI +F   RVFV +     V   
Sbjct: 22 KKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADA 81

Query: 68 PKVG 72
          P +G
Sbjct: 82 PMIG 85

The following BLAST results are available for this feature:

BLAST of Spg031701 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN56897.1	2.6e-21	76.06	hypothetical protein Csa_010510 [Cucumis sativus]	[more]
KAA0049288.1	2.5e-19	72.06	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhib...	[more]
KAA0049287.1	2.5e-19	75.36	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhib...	[more]
KAA0049286.1	1.8e-17	70.42	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhib...	[more]
KAE8650337.1	8.2e-15	69.12	hypothetical protein Csa_010581 [Cucumis sativus]	[more]

BLAST of Spg031701 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	8.5e-07	44.78	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
Q6XNP7	1.6e-05	46.27	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P82381	1.6e-05	43.75	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
Q02214	1.6e-05	41.10	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P20076	3.6e-05	43.75	Ethylene-responsive proteinase inhibitor 1 OS=Solanum lycopersicum OX=4081 PE=3 ...	[more]

BLAST of Spg031701 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8E6	1.3e-21	76.06	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1	[more]
A0A5A7U228	1.2e-19	72.06	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4L9	1.2e-19	75.36	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4U0	8.5e-18	70.42	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0L785	2.1e-16	69.01	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1	[more]

BLAST of Spg031701 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	5.0e-10	53.42	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	3.3e-06	43.08	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	5.7e-06	40.63	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..71 e-value: 3.4E-17 score: 62.9
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 2..71
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 3..70 e-value: 3.1E-20 score: 74.2
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 2..71
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 6..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031701.1	Spg031701.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity