Spg031688 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg031688 vs. NCBI nr
Match: XP_038874856.1 (protein NRT1/ PTR FAMILY 5.2-like [Benincasa hispida])

HSP 1 Score: 949.1 bits (2452), Expect = 1.8e-272
Identity = 484/593 (81.62%), Postives = 535/593 (90.22%), Query Frame = 0

Query: 1   MAATAADQETA--LDDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISV 60
           MA  AADQET   LD++TKDG+VD KGNPVLRSKTG WKACSFIIVYELI+R MF GI+ 
Sbjct: 1   MAGAAADQETGLQLDNYTKDGTVDRKGNPVLRSKTGRWKACSFIIVYELIDRMMFNGIAA 60

Query: 61  NLFIYLTTKLNQGTVTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAM 120
           NL IYLTTKLNQGT+TASNNVTNWTG VWITPILGAY+ADAHLGRYRTF ISS LCL AM
Sbjct: 61  NLIIYLTTKLNQGTLTASNNVTNWTGTVWITPILGAYVADAHLGRYRTFFISSLLCLIAM 120

Query: 121 SLLTLAVSIPSLKPPPCLET-NKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGA 180
           SLLTLAVS+PSLKPPPCLE  +KENCK ASKLQLAVFFGSLYLLALA+GGTKPNISTMGA
Sbjct: 121 SLLTLAVSVPSLKPPPCLEAISKENCKQASKLQLAVFFGSLYLLALASGGTKPNISTMGA 180

Query: 181 DQFDDFHLREKALKLSFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAI 240
           DQFDDF  +EKA KLSFFNWW+FS+F+GILFASTI+VYIQDNVGWSLGYGIP IG+ VAI
Sbjct: 181 DQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPPIGLGVAI 240

Query: 241 VIFVAGTPFYRHRLPNGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFK 300
           +IFV GTPFYRHRLPNGSP T MANVI+AA  NWR PLPNDP EL+ELE++HYSK+GTFK
Sbjct: 241 LIFVVGTPFYRHRLPNGSPFTTMANVIIAAIWNWRFPLPNDPNELYELEIQHYSKHGTFK 300

Query: 301 IDSTPSLRFLNKAAIRRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTL 360
           IDSTPSLRFLNKAAIRRDSS PWR+C+VT+VEETKQM+RMIPI++CTF+PNT+++QS+TL
Sbjct: 301 IDSTPSLRFLNKAAIRRDSSDPWRMCTVTEVEETKQMVRMIPIMICTFIPNTMVAQSHTL 360

Query: 361 FIKQCTTLDRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQ 420
           FIKQ TTLDRSIGSHFK+PPAS Y FVTISML+SI+IYDR+FVKIM+RVTKNPRGITMLQ
Sbjct: 361 FIKQGTTLDRSIGSHFKVPPASLYSFVTISMLLSILIYDRVFVKIMQRVTKNPRGITMLQ 420

Query: 421 RTGIGMIFHVLIMAVAFQVERHRLDVAKEKGLVKNGGQ--LPLTIFILLPQFILTGIAEA 480
           R GIGMI HVLIM VA QVE+HRL +A+E GL     Q  LPLTIFILLPQFILTGIA+A
Sbjct: 421 RMGIGMICHVLIMTVASQVEKHRLHIAEENGLSAAQEQKVLPLTIFILLPQFILTGIADA 480

Query: 481 LLNIAITEFFYDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNL 540
            L IA  EFFYDQAPENMKSLG++Y MTS GIGNFLS+FILSKVS IT RQG GWILNNL
Sbjct: 481 FLQIASNEFFYDQAPENMKSLGNSYFMTSHGIGNFLSTFILSKVSKITKRQGKGWILNNL 540

Query: 541 NASHLDHFYALLAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLK 589
           NASHLD+FYALLAVMSAVNF +FL+ISK+YVY+AEVSDSI+VLTDELKKKK K
Sbjct: 541 NASHLDYFYALLAVMSAVNFGLFLLISKFYVYKAEVSDSIQVLTDELKKKKSK 593

BLAST of Spg031688 vs. NCBI nr
Match: XP_022143759.1 (protein NRT1/ PTR FAMILY 5.2-like [Momordica charantia])

HSP 1 Score: 931.8 bits (2407), Expect = 3.0e-267
Identity = 463/590 (78.47%), Postives = 533/590 (90.34%), Query Frame = 0

Query: 13  DDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGT 72
           DD+T+DGSVDLKGNPVLR KTG WKACSFIIVYE+I+R MF GIS NL IYLTTKLNQGT
Sbjct: 15  DDYTQDGSVDLKGNPVLRRKTGRWKACSFIIVYEVIDRMMFNGISANLIIYLTTKLNQGT 74

Query: 73  VTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKP 132
           VTASNNVTNW+G +WITP+LGAYIADAHLGRYRTFLIS+ LC T+M++LT+AVS+PSLKP
Sbjct: 75  VTASNNVTNWSGTLWITPVLGAYIADAHLGRYRTFLISTLLCFTSMAVLTVAVSVPSLKP 134

Query: 133 PPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKL 192
           PPCLE NKENCK ASKLQL VFFG+LY LA+A+GGTKPNISTMGADQFD+F  +EKA KL
Sbjct: 135 PPCLEANKENCKQASKLQLGVFFGALYALAVASGGTKPNISTMGADQFDEFDPKEKAQKL 194

Query: 193 SFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLP 252
           SFFNWW+FS+F GILFAST++VYIQDNVGW+LGYGIPT G+AVAI+IF+AGTPFYRH+LP
Sbjct: 195 SFFNWWLFSVFFGILFASTVLVYIQDNVGWALGYGIPTSGLAVAILIFLAGTPFYRHKLP 254

Query: 253 NGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI 312
           +GSP +RMA VIVAAA NW LPLP DPK+LHEL+LEHYSK GTF+IDSTPSLR LNKAAI
Sbjct: 255 SGSPFSRMAKVIVAAAFNWTLPLPKDPKQLHELDLEHYSKPGTFRIDSTPSLRILNKAAI 314

Query: 313 RRDSS----HPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDRS 372
           +  SS    HPW+LCSVTQVEETKQMLRMIPIL+CTF+P+T+++Q++TLFIKQ TTLDRS
Sbjct: 315 KTVSSSTTDHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLAQAHTLFIKQGTTLDRS 374

Query: 373 IGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHVL 432
           IGSHFK+PPAS Y FVTISMLV+I++YDR+FVKIM+R+TKNPRGIT+LQR GIGMI HVL
Sbjct: 375 IGSHFKVPPASLYAFVTISMLVTIVVYDRVFVKIMQRLTKNPRGITLLQRMGIGMILHVL 434

Query: 433 IMAVAFQVERHRLDVAKEKGLVKNGGQ--LPLTIFILLPQFILTGIAEALLNIAITEFFY 492
           IM VA +VERHRLDVA+E GLVKNGG   LPL+IF LLPQF+L GIA+A + IA  EFFY
Sbjct: 435 IMTVASRVERHRLDVARENGLVKNGGHAVLPLSIFTLLPQFMLMGIADAFVQIASVEFFY 494

Query: 493 DQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNASHLDHFYAL 552
           DQAPE MKSLGS+Y MTSLGIGNFLSSF+LS VSDIT ++GNGWI NNLNASHLD+FYAL
Sbjct: 495 DQAPETMKSLGSSYMMTSLGIGNFLSSFLLSTVSDITNKRGNGWIQNNLNASHLDYFYAL 554

Query: 553 LAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLKASNDQDEK 597
           LAV++ VNF +FLV+SK YVY+AEVSDSIK+LT+ LKKKKLKAS++QDEK
Sbjct: 555 LAVLNVVNFCIFLVVSKMYVYKAEVSDSIKLLTEGLKKKKLKASHNQDEK 604

BLAST of Spg031688 vs. NCBI nr
Match: XP_031737789.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucumis sativus] >KAE8650326.1 hypothetical protein Csa_011590 [Cucumis sativus])

HSP 1 Score: 922.2 bits (2382), Expect = 2.3e-264
Identity = 467/593 (78.75%), Postives = 529/593 (89.21%), Query Frame = 0

Query: 1   MAATAADQETA---LDDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGIS 60
           MA  AADQE     +D++TKDG+VD KGN VLRSKTG WKACSFI+ YELIER MF GI+
Sbjct: 1   MADAAADQEAGVLDVDNYTKDGTVDWKGNAVLRSKTGRWKACSFILGYELIERMMFNGIA 60

Query: 61  VNLFIYLTTKLNQGTVTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTA 120
            NL IYLTTKLNQGT+TASNNVTNWTG VWI PILGAY+ADAHLGRYRTF ISS +C  A
Sbjct: 61  ANLIIYLTTKLNQGTLTASNNVTNWTGTVWIMPILGAYVADAHLGRYRTFFISSLVCFMA 120

Query: 121 MSLLTLAVSIPSLKPPPC-LETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMG 180
           MSLLTL VSIPSLKPPPC    +KENCK ASKLQLA+FFGSLYLLA+A+GGTKPNISTMG
Sbjct: 121 MSLLTLTVSIPSLKPPPCSTAISKENCKQASKLQLAMFFGSLYLLAIASGGTKPNISTMG 180

Query: 181 ADQFDDFHLREKALKLSFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVA 240
           ADQFDDF  +EKA KLSFFNWW+FS+F+GILFASTI+VYIQDNVGWSLGYGIPTIG+ VA
Sbjct: 181 ADQFDDFDPKEKAQKLSFFNWWLFSVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVA 240

Query: 241 IVIFVAGTPFYRHRLPNGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTF 300
           I+IFV GTPFYRH+LP+GSP  +MANVIVAA  NWRLPLPNDP +L+EL+L+HYSKNGTF
Sbjct: 241 ILIFVVGTPFYRHKLPSGSPFIKMANVIVAATWNWRLPLPNDPNQLYELDLQHYSKNGTF 300

Query: 301 KIDSTPSLRFLNKAAIRRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYT 360
           KIDSTPSLRFLNKAA+R  SS PWR+C+VT+VEETKQM+RMIPI++CTFLP+T+++QS+T
Sbjct: 301 KIDSTPSLRFLNKAALRGVSSDPWRICTVTEVEETKQMVRMIPIMVCTFLPSTMVAQSHT 360

Query: 361 LFIKQCTTLDRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITML 420
           LFIKQ TTLDRSIGSHFK+PPAS Y FVTISML++I+IYDRIFVKIM+RVTKNPRGITML
Sbjct: 361 LFIKQGTTLDRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFVKIMQRVTKNPRGITML 420

Query: 421 QRTGIGMIFHVLIMAVAFQVERHRLDVAKEKG-LVKNGGQLPLTIFILLPQFILTGIAEA 480
           QR GIGMI HVL+M VA QVE+HRL +A + G   +   +LPLTIFILLPQFILTG+A+A
Sbjct: 421 QRMGIGMICHVLVMTVASQVEKHRLHIAAKYGSSAEVQKELPLTIFILLPQFILTGVADA 480

Query: 481 LLNIAITEFFYDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNL 540
            L IA  EFFYDQAPENMKSLGS+Y MTSLGIGNFLSSFILSKVS+IT RQGNGWILNNL
Sbjct: 481 FLQIASNEFFYDQAPENMKSLGSSYFMTSLGIGNFLSSFILSKVSEITKRQGNGWILNNL 540

Query: 541 NASHLDHFYALLAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLK 589
           NASHLD+FYALLAVMS+VN F+FL+ISK+Y+Y+AEVSDSI+VLTDELKKKK K
Sbjct: 541 NASHLDYFYALLAVMSSVNLFIFLLISKFYIYKAEVSDSIQVLTDELKKKKSK 593

BLAST of Spg031688 vs. NCBI nr
Match: XP_011650916.1 (protein NRT1/ PTR FAMILY 5.2 [Cucumis sativus] >KAE8650325.1 hypothetical protein Csa_009670 [Cucumis sativus])

HSP 1 Score: 906.0 bits (2340), Expect = 1.7e-259
Identity = 454/591 (76.82%), Postives = 521/591 (88.16%), Query Frame = 0

Query: 4   TAADQETALDDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIY 63
           T   QE+ +D++T+DG+VDLKG P+LRSKTG WKAC FIIVYEL+ER +F G+S NLFIY
Sbjct: 10  TTTHQESVIDNYTQDGTVDLKGRPLLRSKTGSWKACFFIIVYELLERIVFQGVSANLFIY 69

Query: 64  LTTKLNQGTVTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTL 123
           LTTKL+QG VTASNNVTNW GA+WI PI GAYIADAHLGRYRTFLISS +  TAMSLLTL
Sbjct: 70  LTTKLHQGIVTASNNVTNWNGAIWIMPIFGAYIADAHLGRYRTFLISSFIWFTAMSLLTL 129

Query: 124 AVSIPSLKPPPCLE-TNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDD 183
           AVS+PSLKPPPCL+   K+NCK ASKLQLAVFFGSLYLL +A+GGTKPNISTMGADQFDD
Sbjct: 130 AVSVPSLKPPPCLQPITKQNCKQASKLQLAVFFGSLYLLVIASGGTKPNISTMGADQFDD 189

Query: 184 FHLREKALKLSFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVA 243
           F+ +EKA KLSFFNWW F+ F+GILFASTI+VYIQDNVGWSLGYGIPTIG+ VAI+IFV 
Sbjct: 190 FYPKEKAQKLSFFNWWFFTAFSGILFASTILVYIQDNVGWSLGYGIPTIGIGVAIIIFVV 249

Query: 244 GTPFYRHRLPNGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTP 303
           GTPFYRHR P+GSP T +ANVIV AA NWRLP+PNDP +L+ELE++HYSK G FKIDSTP
Sbjct: 250 GTPFYRHRPPSGSPFTTIANVIVGAAWNWRLPIPNDPNQLYELEIQHYSKPGNFKIDSTP 309

Query: 304 SLRFLNKAAIRRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQC 363
           SLRFLNKAAIRRDS  PWRLCSVT+VEETKQMLRMIPIL+CTF+P+TI +Q++TLFIKQ 
Sbjct: 310 SLRFLNKAAIRRDSGGPWRLCSVTEVEETKQMLRMIPILICTFIPHTITAQTHTLFIKQG 369

Query: 364 TTLDRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIG 423
           TTLDRSIGSHFK+PPAS Y FVTIS+L+SI+IYDRIFVKI++RVTKNPRGITMLQR GIG
Sbjct: 370 TTLDRSIGSHFKVPPASLYAFVTISLLLSILIYDRIFVKIVRRVTKNPRGITMLQRMGIG 429

Query: 424 MIFHVLIMAVAFQVERHRLDV-AKEKGLVKNGGQ-LPLTIFILLPQFILTGIAEALLNIA 483
           MI HVL+M VA +VE+HRLDV A+E G      + LPLTIF LLPQFILTG+A++ L +A
Sbjct: 430 MICHVLVMVVASRVEKHRLDVAARENGSSPQEQKVLPLTIFTLLPQFILTGVADSFLQVA 489

Query: 484 ITEFFYDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNASHL 543
           + EFFYDQAPENMKS G++Y MTSLGIGNFLSS I+SKVS+IT RQG  WILNNLNASHL
Sbjct: 490 VQEFFYDQAPENMKSFGTSYAMTSLGIGNFLSSLIVSKVSEITKRQGKEWILNNLNASHL 549

Query: 544 DHFYALLAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLKASN 592
           D+FY LLAVMSAVNFF+FL+ISK Y+Y+AEVSDSI +LT+ELKKKK  A+N
Sbjct: 550 DYFYLLLAVMSAVNFFLFLIISKLYLYKAEVSDSINLLTEELKKKKSMATN 600

BLAST of Spg031688 vs. NCBI nr
Match: XP_022924429.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 905.6 bits (2339), Expect = 2.3e-259
Identity = 446/590 (75.59%), Postives = 525/590 (88.98%), Query Frame = 0

Query: 1   MAATAADQETALDDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNL 60
           MAA+AA +E+ +DD+TKDG+VDLKGNPVLRSK G WKACSFI+VYE+ ER  + GIS NL
Sbjct: 1   MAASAA-EESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNL 60

Query: 61  FIYLTTKLNQGTVTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSL 120
            I+LT KL+QGTV ++NNVTNW+G VWI PILGAYIADAHLGRYRTFLI+SA+CLT M L
Sbjct: 61  IIFLTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGL 120

Query: 121 LTLAVSIPSLKPPPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQF 180
           LTLAVS+PSLKPPPCL+ NK NCK AS LQLAVFFG+LY+LAL TGGTKPNIST+GADQF
Sbjct: 121 LTLAVSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQF 180

Query: 181 DDFHLREKALKLSFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIF 240
           D+FH +EKA KLSFFNWWMFSIF G LFA+TI+VYIQDNVGWSLGYG+PTIG+A++I+IF
Sbjct: 181 DEFHPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIF 240

Query: 241 VAGTPFYRHRLPNGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDS 300
           VAGTPFYRH+LP GSP T+MA+VIVAA RNWRLPLPNDPKELHEL  E Y+K G F+IDS
Sbjct: 241 VAGTPFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDS 300

Query: 301 TPSLRFLNKAAIRRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIK 360
           TPSLRFLNKAAIRR SS  W+LC+VTQVEETKQMLRMIP+L+CTF+P+T+++Q++TLFIK
Sbjct: 301 TPSLRFLNKAAIRRGSSDSWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLFIK 360

Query: 361 QCTTLDRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTG 420
           Q TTLDRSIGSHF+IPPAS   FVTISML+S++IYDR+FVKIM+R+TKNPRGIT+LQR G
Sbjct: 361 QGTTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQRMG 420

Query: 421 IGMIFHVLIMAVAFQVERHRLDVAKEKGLVKN--GGQLPLTIFILLPQFILTGIAEALLN 480
           IGMI HVLIM +A +VERHRLDVA++ G V N  G  LPLTIF LLPQF+L G+A+A   
Sbjct: 421 IGMILHVLIMIIASRVERHRLDVARQNGQVGNASGQALPLTIFTLLPQFMLVGVADAFTE 480

Query: 481 IAITEFFYDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNAS 540
           +A  EFFYDQAPE+MKSLG++Y+MTS+GIGNFLSSF+LS VS IT ++GNGWI+NNLNAS
Sbjct: 481 VAKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKRGNGWIMNNLNAS 540

Query: 541 HLDHFYALLAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLK 589
           HLD++YA LAV+SA+NFF+FL+ISK+YVY+AEVS SIKVL DELK KKLK
Sbjct: 541 HLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKVLADELKDKKLK 589

BLAST of Spg031688 vs. ExPASy Swiss-Prot
Match: Q9FNL7 (Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 1.7e-209
Identity = 356/573 (62.13%), Postives = 455/573 (79.41%), Query Frame = 0

Query: 13  DDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGT 72
           DD+TKDG+VDL+GNPV RS  G WKACSF++VYE+ ER  + GIS NLFIY+TTKL+QGT
Sbjct: 8   DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTTKLHQGT 67

Query: 73  VTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKP 132
           V +SNNVTNW G  W+TPILGAY+ DA LGRY TF+IS A+  + M +LTL+V+IP +KP
Sbjct: 68  VKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVTIPGIKP 127

Query: 133 PPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKL 192
           P C  TN ENC+ AS LQLAVFFG+LY LA+ TGGTKPNIST+GADQFD F  +EK  KL
Sbjct: 128 PECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPKEKTQKL 187

Query: 193 SFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLP 252
           SFFNWWMFSIF G LFA+T++VY+QDNVGW+LGYG+PT+G+A++I IF+ GTPFYRH+LP
Sbjct: 188 SFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPFYRHKLP 247

Query: 253 NGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI 312
            GSP T+MA VIVA+ R    P+ +D    HEL    Y + G F I  TPSLRFL++A++
Sbjct: 248 TGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRFLDRASL 307

Query: 313 RRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDRSIGSH 372
           +  ++H W LC+ T+VEETKQMLRM+P+L  TF+P+ +++Q  TLF+KQ TTLDR +   
Sbjct: 308 KTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGTTLDRKVTGS 367

Query: 373 FKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHVLIMAV 432
           F IPPAS   FVT+SML+SI++YDR+FVKI ++ T NPRGIT+LQR GIG+IFH+LIM V
Sbjct: 368 FSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIGLIFHILIMIV 427

Query: 433 AFQVERHRLDVAKEKGLV-KNGGQLPLTIFILLPQFILTGIAEALLNIAITEFFYDQAPE 492
           A   ER+RL VA + GL+ + G +LPLTIF LLPQF+L G+A++ L +A  EFFYDQAPE
Sbjct: 428 ASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAKLEFFYDQAPE 487

Query: 493 NMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNASHLDHFYALLAVMS 552
           +MKSLG++Y+ TSL IGNF+SSF+LS VS+IT ++G GWILNNLN S LD++Y   AV++
Sbjct: 488 SMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESRLDYYYLFFAVLN 547

Query: 553 AVNFFVFLVISKWYVYRAEVSDSIKVLTDELKK 585
            VNF +FLV+ K+YVYRAEV+DS+ V   E+K+
Sbjct: 548 LVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of Spg031688 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 2.1e-199
Identity = 341/566 (60.25%), Postives = 440/566 (77.74%), Query Frame = 0

Query: 13  DDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGT 72
           DD+TKDG+VDL+GN V RS+TG WKACSF++VYE+ ER  + GIS NL IY+TTKL+QGT
Sbjct: 8   DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTTKLHQGT 67

Query: 73  VTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKP 132
           V +SNNVTNW G  W+TPILGAY+ADAH GRY TF+ISSA+ L  M+LLTL+VS+P LKP
Sbjct: 68  VKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVSLPGLKP 127

Query: 133 PPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKL 192
           P C   N ENC+ AS +QLAVFFG+LY LA+ TGGTKPNIST+GADQFD+F  ++K  K 
Sbjct: 128 PKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPKDKIHKH 187

Query: 193 SFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLP 252
           SFFNWWMFSIF G  FA+T++VY+QDNVGW++GYG+ T+G+A +I IF+ GT  YRH+LP
Sbjct: 188 SFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRLYRHKLP 247

Query: 253 NGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI 312
            GSP T+MA VIVA+ R  R P+ +D    +EL    Y+    F I ST SLRFLN+A++
Sbjct: 248 MGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRFLNRASL 307

Query: 313 RRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDRSIGSH 372
           +  S+H WRLC++T+VEETKQML+M+P+L  TF+P+ +++Q  TLFIKQ TTLDR + ++
Sbjct: 308 KTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGTTLDRRLTNN 367

Query: 373 FKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHVLIMAV 432
           F IPPAS   F T SMLVSI+IYDR+FVK M+++T NPRGIT+LQR GIGMI H+LIM +
Sbjct: 368 FSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIGMILHILIMII 427

Query: 433 AFQVERHRLDVAKEKGLV-KNGGQLPLTIFILLPQFILTGIAEALLNIAITEFFYDQAPE 492
           A   ER+RL VA E GL  +    +PL+IF LLPQ++L G+A+A + IA  EFFYDQAPE
Sbjct: 428 ASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADAFIEIAKLEFFYDQAPE 487

Query: 493 NMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNASHLDHFYALLAVMS 552
           +MKSLG++YT TS+ +G F+SS +LS VS IT +QG GWI NNLN S LD++Y   AV++
Sbjct: 488 SMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESRLDNYYMFFAVLN 547

Query: 553 AVNFFVFLVISKWYVYRAEVSDSIKV 578
            +NF +FLV+ ++Y YRA+V+ S  V
Sbjct: 548 LLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Spg031688 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 533.9 bits (1374), Expect = 2.4e-150
Identity = 275/558 (49.28%), Postives = 387/558 (69.35%), Query Frame = 0

Query: 15  FTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGTVT 74
           +T+DG+VDL+G PVL SKTG W+ACSF++ YE  ER  F GI+ NL  YLT +L++ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 75  ASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKPPP 134
           +  NV NW+GAVWITPI GAYIAD+++GR+ TF  SS + +  M LLT+AV++ SL+P  
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRP-- 126

Query: 135 CLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKLSF 194
                   C  AS LQ+  F+ SLY +A+  GGTKPNIST GADQFD + + EK  K+SF
Sbjct: 127 --TCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSF 186

Query: 195 FNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLPNG 254
           FNWWMFS F G LFA+  +VYIQ+N+GW LGYGIPT+G+ V++V+F  GTPFYRH++   
Sbjct: 187 FNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKT 246

Query: 255 SPITR-MANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAIR 314
             + + +  V +AA +N +L  P+D  EL+EL+  +Y  NG  ++  TP  RFL+KAAI+
Sbjct: 247 DNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAIK 306

Query: 315 RDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDRSIGSHF 374
             S  P   C+VT+VE  K++L +I I L T +P+T+ +Q  TLF+KQ TTLDR IGS+F
Sbjct: 307 TSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGSNF 366

Query: 375 KIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHVLIMAVA 434
           +IP AS   FVT+SML+S+ +YD+ FV  M++ T NPRGIT+LQR G+G    ++ +A+A
Sbjct: 367 QIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIAIA 426

Query: 435 FQVERHRLDVAKEKGLVKNGGQLPLTIFILLPQFILTGIAEALLNIAITEFFYDQAPENM 494
             VE  R+ V KE  +      +P++IF LLPQ+ L GI +    I + EFFYDQ+PE M
Sbjct: 427 SAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQSPEEM 486

Query: 495 KSLGSTYTMTSLGIGNFLSSFILSKVSDITIR-QGNGWILNNLNASHLDHFYALLAVMSA 554
           +SLG+T+  + +G+GNFL+SF+++ +  IT +  G  WI NNLN S LD++Y  L V+S 
Sbjct: 487 QSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFLVVISI 546

Query: 555 VNFFVFLVISKWYVYRAE 571
           VN  +F+  +  YVY+++
Sbjct: 547 VNMGLFVWAASKYVYKSD 557

BLAST of Spg031688 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 2.7e-130
Identity = 251/567 (44.27%), Postives = 363/567 (64.02%), Query Frame = 0

Query: 13  DDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGT 72
           D +T+DG+VD+  NP  + KTG WKAC FI+  E  ER  + G+  NL  YL ++LNQG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 73  VTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKP 132
            TA+NNVTNW+G  +ITP++GA+IADA+LGRY T      + ++ M+LLTL+ S+P LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 133 PPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKL 192
             C   N + C H +  Q AVFF +LY++AL TGG KP +S+ GADQFD+    EK  K 
Sbjct: 125 GNC---NADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 184

Query: 193 SFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLP 252
           SFFNW+ FSI  G L A+T++V+IQ NVGW  G+G+PT+ + +A+  F  G+ FYR + P
Sbjct: 185 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 244

Query: 253 NGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI 312
            GSP+TR+  VIVAA R   + +P D   L E   +  +  G+ K+  T +L+F +KAA+
Sbjct: 245 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 304

Query: 313 RRDSS-------HPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTL 372
              S        +PWRLCSVTQVEE K ++ ++P+     +  T+ SQ  T+F+ Q  T+
Sbjct: 305 ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 364

Query: 373 DRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIF 432
           D+ +G +F+IP AS  +F T+S+L    +YD+  + + ++ T+N RG T LQR GIG++ 
Sbjct: 365 DQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVV 424

Query: 433 HVLIMAVAFQVERHRLDVAKEKGLVKNGGQLPLTIFILLPQFILTGIAEALLNIAITEFF 492
            +  M  A  +E  RLD  K      +  Q+ ++IF  +PQ++L G AE    I   EFF
Sbjct: 425 SIFAMITAGVLEVVRLDYVKTHN-AYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 484

Query: 493 YDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGN-GWILNNLNASHLDHFY 552
           YDQAP+ M+SL S  ++T++ +GN+LS+ +++ V  IT + G  GWI +NLN  HLD+F+
Sbjct: 485 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFF 544

Query: 553 ALLAVMSAVNFFVFLVISKWYVYRAEV 572
            LLA +S +NF V+L ISK Y Y+  V
Sbjct: 545 YLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Spg031688 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.9e-128
Identity = 249/559 (44.54%), Postives = 359/559 (64.22%), Query Frame = 0

Query: 15  FTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGTVT 74
           + +DGSVD  GNP L+ KTG WKAC FI+  E  ER  + GI+ NL  YLTTKL+QG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 75  ASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKPPP 134
           A+ NVT W G  ++TP++GA +ADA+ GRY T    S +    MS LTL+ S+P+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 135 CLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKLSF 194
           C+    + C  A+  Q A+FFG LYL+AL TGG KP +S+ GADQFDD   RE+  K SF
Sbjct: 144 CI---GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 203

Query: 195 FNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLPNG 254
           FNW+ FSI  G L +S+++V+IQ+N GW LG+GIPT+ + +AI  F  GTP YR + P G
Sbjct: 204 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 263

Query: 255 SPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI-- 314
           SPITR++ V+VA+ R   + +P D   L+E + ++ +  G+ KI+ T   ++L+KAA+  
Sbjct: 264 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 323

Query: 315 -----RRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDR 374
                  D S+ WRLC+VTQVEE K ++RM PI     + + + +Q  T+F++Q   ++ 
Sbjct: 324 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 383

Query: 375 SIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHV 434
            IGS F++PPA+   F T S+++ + +YDR  V + ++ T   +G T +QR GIG+   V
Sbjct: 384 KIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSV 443

Query: 435 LIMAVAFQVERHRLDVAKEKGLVKNGGQLPLTIFILLPQFILTGIAEALLNIAITEFFYD 494
           L MA A  VE  RL +A + GLV++G  +P+++   +PQ+ + G AE    I   EFFYD
Sbjct: 444 LCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYD 503

Query: 495 QAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQG-NGWILNNLNASHLDHFYAL 554
           Q+P+ M+SL S   + +  +GN+LSS IL+ V+  T R G  GWI +NLN+ HLD+F+ L
Sbjct: 504 QSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWL 563

Query: 555 LAVMSAVNFFVFLVISKWY 566
           LA +S VN  V+   +  Y
Sbjct: 564 LAGLSLVNMAVYFFSAARY 578

BLAST of Spg031688 vs. ExPASy TrEMBL
Match: A0A6J1CQA1 (protein NRT1/ PTR FAMILY 5.2-like OS=Momordica charantia OX=3673 GN=LOC111013596 PE=3 SV=1)

HSP 1 Score: 931.8 bits (2407), Expect = 1.4e-267
Identity = 463/590 (78.47%), Postives = 533/590 (90.34%), Query Frame = 0

Query: 13  DDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGT 72
           DD+T+DGSVDLKGNPVLR KTG WKACSFIIVYE+I+R MF GIS NL IYLTTKLNQGT
Sbjct: 15  DDYTQDGSVDLKGNPVLRRKTGRWKACSFIIVYEVIDRMMFNGISANLIIYLTTKLNQGT 74

Query: 73  VTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKP 132
           VTASNNVTNW+G +WITP+LGAYIADAHLGRYRTFLIS+ LC T+M++LT+AVS+PSLKP
Sbjct: 75  VTASNNVTNWSGTLWITPVLGAYIADAHLGRYRTFLISTLLCFTSMAVLTVAVSVPSLKP 134

Query: 133 PPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKL 192
           PPCLE NKENCK ASKLQL VFFG+LY LA+A+GGTKPNISTMGADQFD+F  +EKA KL
Sbjct: 135 PPCLEANKENCKQASKLQLGVFFGALYALAVASGGTKPNISTMGADQFDEFDPKEKAQKL 194

Query: 193 SFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLP 252
           SFFNWW+FS+F GILFAST++VYIQDNVGW+LGYGIPT G+AVAI+IF+AGTPFYRH+LP
Sbjct: 195 SFFNWWLFSVFFGILFASTVLVYIQDNVGWALGYGIPTSGLAVAILIFLAGTPFYRHKLP 254

Query: 253 NGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI 312
           +GSP +RMA VIVAAA NW LPLP DPK+LHEL+LEHYSK GTF+IDSTPSLR LNKAAI
Sbjct: 255 SGSPFSRMAKVIVAAAFNWTLPLPKDPKQLHELDLEHYSKPGTFRIDSTPSLRILNKAAI 314

Query: 313 RRDSS----HPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDRS 372
           +  SS    HPW+LCSVTQVEETKQMLRMIPIL+CTF+P+T+++Q++TLFIKQ TTLDRS
Sbjct: 315 KTVSSSTTDHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLAQAHTLFIKQGTTLDRS 374

Query: 373 IGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHVL 432
           IGSHFK+PPAS Y FVTISMLV+I++YDR+FVKIM+R+TKNPRGIT+LQR GIGMI HVL
Sbjct: 375 IGSHFKVPPASLYAFVTISMLVTIVVYDRVFVKIMQRLTKNPRGITLLQRMGIGMILHVL 434

Query: 433 IMAVAFQVERHRLDVAKEKGLVKNGGQ--LPLTIFILLPQFILTGIAEALLNIAITEFFY 492
           IM VA +VERHRLDVA+E GLVKNGG   LPL+IF LLPQF+L GIA+A + IA  EFFY
Sbjct: 435 IMTVASRVERHRLDVARENGLVKNGGHAVLPLSIFTLLPQFMLMGIADAFVQIASVEFFY 494

Query: 493 DQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNASHLDHFYAL 552
           DQAPE MKSLGS+Y MTSLGIGNFLSSF+LS VSDIT ++GNGWI NNLNASHLD+FYAL
Sbjct: 495 DQAPETMKSLGSSYMMTSLGIGNFLSSFLLSTVSDITNKRGNGWIQNNLNASHLDYFYAL 554

Query: 553 LAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLKASNDQDEK 597
           LAV++ VNF +FLV+SK YVY+AEVSDSIK+LT+ LKKKKLKAS++QDEK
Sbjct: 555 LAVLNVVNFCIFLVVSKMYVYKAEVSDSIKLLTEGLKKKKLKASHNQDEK 604

BLAST of Spg031688 vs. ExPASy TrEMBL
Match: A0A0A0L9Q0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G134750 PE=3 SV=1)

HSP 1 Score: 906.0 bits (2340), Expect = 8.4e-260
Identity = 454/591 (76.82%), Postives = 521/591 (88.16%), Query Frame = 0

Query: 4   TAADQETALDDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIY 63
           T   QE+ +D++T+DG+VDLKG P+LRSKTG WKAC FIIVYEL+ER +F G+S NLFIY
Sbjct: 5   TTTHQESVIDNYTQDGTVDLKGRPLLRSKTGSWKACFFIIVYELLERIVFQGVSANLFIY 64

Query: 64  LTTKLNQGTVTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTL 123
           LTTKL+QG VTASNNVTNW GA+WI PI GAYIADAHLGRYRTFLISS +  TAMSLLTL
Sbjct: 65  LTTKLHQGIVTASNNVTNWNGAIWIMPIFGAYIADAHLGRYRTFLISSFIWFTAMSLLTL 124

Query: 124 AVSIPSLKPPPCLE-TNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDD 183
           AVS+PSLKPPPCL+   K+NCK ASKLQLAVFFGSLYLL +A+GGTKPNISTMGADQFDD
Sbjct: 125 AVSVPSLKPPPCLQPITKQNCKQASKLQLAVFFGSLYLLVIASGGTKPNISTMGADQFDD 184

Query: 184 FHLREKALKLSFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVA 243
           F+ +EKA KLSFFNWW F+ F+GILFASTI+VYIQDNVGWSLGYGIPTIG+ VAI+IFV 
Sbjct: 185 FYPKEKAQKLSFFNWWFFTAFSGILFASTILVYIQDNVGWSLGYGIPTIGIGVAIIIFVV 244

Query: 244 GTPFYRHRLPNGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTP 303
           GTPFYRHR P+GSP T +ANVIV AA NWRLP+PNDP +L+ELE++HYSK G FKIDSTP
Sbjct: 245 GTPFYRHRPPSGSPFTTIANVIVGAAWNWRLPIPNDPNQLYELEIQHYSKPGNFKIDSTP 304

Query: 304 SLRFLNKAAIRRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQC 363
           SLRFLNKAAIRRDS  PWRLCSVT+VEETKQMLRMIPIL+CTF+P+TI +Q++TLFIKQ 
Sbjct: 305 SLRFLNKAAIRRDSGGPWRLCSVTEVEETKQMLRMIPILICTFIPHTITAQTHTLFIKQG 364

Query: 364 TTLDRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIG 423
           TTLDRSIGSHFK+PPAS Y FVTIS+L+SI+IYDRIFVKI++RVTKNPRGITMLQR GIG
Sbjct: 365 TTLDRSIGSHFKVPPASLYAFVTISLLLSILIYDRIFVKIVRRVTKNPRGITMLQRMGIG 424

Query: 424 MIFHVLIMAVAFQVERHRLDV-AKEKGLVKNGGQ-LPLTIFILLPQFILTGIAEALLNIA 483
           MI HVL+M VA +VE+HRLDV A+E G      + LPLTIF LLPQFILTG+A++ L +A
Sbjct: 425 MICHVLVMVVASRVEKHRLDVAARENGSSPQEQKVLPLTIFTLLPQFILTGVADSFLQVA 484

Query: 484 ITEFFYDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNASHL 543
           + EFFYDQAPENMKS G++Y MTSLGIGNFLSS I+SKVS+IT RQG  WILNNLNASHL
Sbjct: 485 VQEFFYDQAPENMKSFGTSYAMTSLGIGNFLSSLIVSKVSEITKRQGKEWILNNLNASHL 544

Query: 544 DHFYALLAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLKASN 592
           D+FY LLAVMSAVNFF+FL+ISK Y+Y+AEVSDSI +LT+ELKKKK  A+N
Sbjct: 545 DYFYLLLAVMSAVNFFLFLIISKLYLYKAEVSDSINLLTEELKKKKSMATN 595

BLAST of Spg031688 vs. ExPASy TrEMBL
Match: A0A6J1E956 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431935 PE=3 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 1.1e-259
Identity = 446/590 (75.59%), Postives = 525/590 (88.98%), Query Frame = 0

Query: 1   MAATAADQETALDDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNL 60
           MAA+AA +E+ +DD+TKDG+VDLKGNPVLRSK G WKACSFI+VYE+ ER  + GIS NL
Sbjct: 1   MAASAA-EESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNL 60

Query: 61  FIYLTTKLNQGTVTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSL 120
            I+LT KL+QGTV ++NNVTNW+G VWI PILGAYIADAHLGRYRTFLI+SA+CLT M L
Sbjct: 61  IIFLTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGL 120

Query: 121 LTLAVSIPSLKPPPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQF 180
           LTLAVS+PSLKPPPCL+ NK NCK AS LQLAVFFG+LY+LAL TGGTKPNIST+GADQF
Sbjct: 121 LTLAVSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQF 180

Query: 181 DDFHLREKALKLSFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIF 240
           D+FH +EKA KLSFFNWWMFSIF G LFA+TI+VYIQDNVGWSLGYG+PTIG+A++I+IF
Sbjct: 181 DEFHPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIF 240

Query: 241 VAGTPFYRHRLPNGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDS 300
           VAGTPFYRH+LP GSP T+MA+VIVAA RNWRLPLPNDPKELHEL  E Y+K G F+IDS
Sbjct: 241 VAGTPFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDS 300

Query: 301 TPSLRFLNKAAIRRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIK 360
           TPSLRFLNKAAIRR SS  W+LC+VTQVEETKQMLRMIP+L+CTF+P+T+++Q++TLFIK
Sbjct: 301 TPSLRFLNKAAIRRGSSDSWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLFIK 360

Query: 361 QCTTLDRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTG 420
           Q TTLDRSIGSHF+IPPAS   FVTISML+S++IYDR+FVKIM+R+TKNPRGIT+LQR G
Sbjct: 361 QGTTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQRMG 420

Query: 421 IGMIFHVLIMAVAFQVERHRLDVAKEKGLVKN--GGQLPLTIFILLPQFILTGIAEALLN 480
           IGMI HVLIM +A +VERHRLDVA++ G V N  G  LPLTIF LLPQF+L G+A+A   
Sbjct: 421 IGMILHVLIMIIASRVERHRLDVARQNGQVGNASGQALPLTIFTLLPQFMLVGVADAFTE 480

Query: 481 IAITEFFYDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNAS 540
           +A  EFFYDQAPE+MKSLG++Y+MTS+GIGNFLSSF+LS VS IT ++GNGWI+NNLNAS
Sbjct: 481 VAKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKRGNGWIMNNLNAS 540

Query: 541 HLDHFYALLAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLK 589
           HLD++YA LAV+SA+NFF+FL+ISK+YVY+AEVS SIKVL DELK KKLK
Sbjct: 541 HLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKVLADELKDKKLK 589

BLAST of Spg031688 vs. ExPASy TrEMBL
Match: A0A6J1IQK5 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479112 PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 2.5e-259
Identity = 447/595 (75.13%), Postives = 528/595 (88.74%), Query Frame = 0

Query: 1   MAATAADQETALDDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNL 60
           MAA+AA +E+ +DD+TKDG+VDLKGNPVLRSK G WKACSFI+VYE+ ER  + GIS NL
Sbjct: 1   MAASAA-EESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNL 60

Query: 61  FIYLTTKLNQGTVTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSL 120
            I+LT KL+QGTV ++NNVTNW+G VWI PILGAYIADAHLGRYRTFLI+SA+CLT M L
Sbjct: 61  IIFLTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGL 120

Query: 121 LTLAVSIPSLKPPPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQF 180
           LTLAVS+PSLKPPPCL+ NK NCK AS LQLAVFFG+LY+LAL TGGTKPNIST+GADQF
Sbjct: 121 LTLAVSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQF 180

Query: 181 DDFHLREKALKLSFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIF 240
           D+FH +EKA KLSFFNWWMFSIF G LFA+TI+VYIQDNVGWSLGYG+PTIG+A++I+IF
Sbjct: 181 DEFHPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIF 240

Query: 241 VAGTPFYRHRLPNGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDS 300
           VAGTPFYRH+LP GSP T+MA+VIVAA RNWRLPLPNDPKELHEL  E Y+K G F+IDS
Sbjct: 241 VAGTPFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDS 300

Query: 301 TPSLRFLNKAAIRRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIK 360
           TPSLRFLNKAAIRR SS  W+LC+VTQVEETKQMLRMIP+L+CTF+P+T+++Q++TLFIK
Sbjct: 301 TPSLRFLNKAAIRRGSSDSWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLFIK 360

Query: 361 QCTTLDRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTG 420
           Q TTLDRSIGSHF+IPPAS   FVTISML+S++IYDR+FVKIM+R+TKNPRGIT+LQR G
Sbjct: 361 QGTTLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQRMG 420

Query: 421 IGMIFHVLIMAVAFQVERHRLDVAKEKGLVKNG-GQ-LPLTIFILLPQFILTGIAEALLN 480
           IGMI HVLIM +A +VERHRLDVA++ G V NG GQ LPLTIF LLPQF+L G+A+A   
Sbjct: 421 IGMILHVLIMIIASRVERHRLDVARQNGQVGNGSGQALPLTIFTLLPQFMLVGVADAFTE 480

Query: 481 IAITEFFYDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNAS 540
           +A  EFFYDQAPE+MKSLG++Y+MTS+GIGNFLSSF+LS VS IT + GNGWI+NNLNAS
Sbjct: 481 VAKIEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKHGNGWIMNNLNAS 540

Query: 541 HLDHFYALLAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLKASNDQ 594
           HLD++YA LAV+SA+NFF+FL+ISK+YVY+AEVS SIK L DELK KKLK  +++
Sbjct: 541 HLDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKALADELKDKKLKPFSEK 594

BLAST of Spg031688 vs. ExPASy TrEMBL
Match: A0A5A7U6G8 (Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G004610 PE=3 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.2e-258
Identity = 457/593 (77.07%), Postives = 525/593 (88.53%), Query Frame = 0

Query: 1   MAATAADQETALD--DFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISV 60
           MA  AA+QE  LD  ++TKDG+VD KGN VLRSKTG WKACSFI+ YELIER MF GIS 
Sbjct: 1   MADAAANQEAGLDLHNYTKDGTVDWKGNTVLRSKTGRWKACSFILGYELIERMMFHGISS 60

Query: 61  NLFIYLTTKLNQGTVTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAM 120
           NL IYLTTKLNQGT+TASNNVTNW+G VW  PI+GAY+ADAHLGRYRTF ISS +C  AM
Sbjct: 61  NLIIYLTTKLNQGTLTASNNVTNWSGTVWTMPIIGAYVADAHLGRYRTFFISSLVCFMAM 120

Query: 121 SLLTLAVSIPSLKPPPC-LETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGA 180
           +LLT AVSIPSLKPPPC    ++ENCK ASKLQLAVFFGSLYLLA+A+GGTKPNISTMGA
Sbjct: 121 TLLTAAVSIPSLKPPPCSASISRENCKQASKLQLAVFFGSLYLLAIASGGTKPNISTMGA 180

Query: 181 DQFDDFHLREKALKLSFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAI 240
           DQFDDF  +EKA KLSFFNWW+F++F+GILFASTI+VYIQDNVGWSLGYGIPTIG+ VAI
Sbjct: 181 DQFDDFDPKEKAQKLSFFNWWLFTVFSGILFASTILVYIQDNVGWSLGYGIPTIGLGVAI 240

Query: 241 VIFVAGTPFYRHRLPNGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFK 300
           +IFV GTPFYRH+ PNGSP   MANV VAA  NWRLPLPNDP +LHEL+L++YSKNGTFK
Sbjct: 241 LIFVVGTPFYRHKPPNGSPFITMANVFVAAIWNWRLPLPNDPNQLHELDLQNYSKNGTFK 300

Query: 301 IDSTPSLRFLNKAAIRRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTL 360
           IDSTPSLRFLNKAAIRR SS PWR+C+VT+VEETKQM+RMIPI++C+F+P+ +++Q++TL
Sbjct: 301 IDSTPSLRFLNKAAIRRVSSDPWRICTVTEVEETKQMVRMIPIMVCSFIPSAMVAQTHTL 360

Query: 361 FIKQCTTLDRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQ 420
           FIKQ TTL+RSIGSHFK+PPAS Y FVTISML++I+IYDRIF+KIM+RVTKNPRGITMLQ
Sbjct: 361 FIKQGTTLNRSIGSHFKVPPASLYAFVTISMLLTILIYDRIFLKIMQRVTKNPRGITMLQ 420

Query: 421 RTGIGMIFHVLIMAVAFQVERHRLDVAKEKG-LVKNGGQLPLTIFILLPQFILTGIAEAL 480
           R GIGMI HVL+M VA QVE+HRL +A + G       +LPLTIFILLPQFILTG+A+A 
Sbjct: 421 RMGIGMICHVLVMTVASQVEKHRLHIAAKYGSSAHEQKELPLTIFILLPQFILTGVADAF 480

Query: 481 LNIAITEFFYDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLN 540
           L IA  EFFYDQAPENMKSLGS+Y  TSLGIGNFLS+FILSKVS+IT RQGNGWILNNLN
Sbjct: 481 LLIANNEFFYDQAPENMKSLGSSYFTTSLGIGNFLSTFILSKVSEITKRQGNGWILNNLN 540

Query: 541 ASHLDHFYALLAVMSAVNFFVFLVISKWYVYRAEVSDSIKVLTDELKKKKLKA 590
           +SHL++FYALLAVMS+VNFF+FL+ISK+YVY+AEVSDSI+VLTDELKKKK KA
Sbjct: 541 SSHLNYFYALLAVMSSVNFFLFLLISKFYVYKAEVSDSIQVLTDELKKKKSKA 593

BLAST of Spg031688 vs. TAIR 10
Match: AT5G46050.1 (peptide transporter 3 )

HSP 1 Score: 730.3 bits (1884), Expect = 1.2e-210
Identity = 356/573 (62.13%), Postives = 455/573 (79.41%), Query Frame = 0

Query: 13  DDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGT 72
           DD+TKDG+VDL+GNPV RS  G WKACSF++VYE+ ER  + GIS NLFIY+TTKL+QGT
Sbjct: 8   DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTTKLHQGT 67

Query: 73  VTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKP 132
           V +SNNVTNW G  W+TPILGAY+ DA LGRY TF+IS A+  + M +LTL+V+IP +KP
Sbjct: 68  VKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVTIPGIKP 127

Query: 133 PPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKL 192
           P C  TN ENC+ AS LQLAVFFG+LY LA+ TGGTKPNIST+GADQFD F  +EK  KL
Sbjct: 128 PECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPKEKTQKL 187

Query: 193 SFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLP 252
           SFFNWWMFSIF G LFA+T++VY+QDNVGW+LGYG+PT+G+A++I IF+ GTPFYRH+LP
Sbjct: 188 SFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPFYRHKLP 247

Query: 253 NGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI 312
            GSP T+MA VIVA+ R    P+ +D    HEL    Y + G F I  TPSLRFL++A++
Sbjct: 248 TGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRFLDRASL 307

Query: 313 RRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDRSIGSH 372
           +  ++H W LC+ T+VEETKQMLRM+P+L  TF+P+ +++Q  TLF+KQ TTLDR +   
Sbjct: 308 KTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGTTLDRKVTGS 367

Query: 373 FKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHVLIMAV 432
           F IPPAS   FVT+SML+SI++YDR+FVKI ++ T NPRGIT+LQR GIG+IFH+LIM V
Sbjct: 368 FSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIGLIFHILIMIV 427

Query: 433 AFQVERHRLDVAKEKGLV-KNGGQLPLTIFILLPQFILTGIAEALLNIAITEFFYDQAPE 492
           A   ER+RL VA + GL+ + G +LPLTIF LLPQF+L G+A++ L +A  EFFYDQAPE
Sbjct: 428 ASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAKLEFFYDQAPE 487

Query: 493 NMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNASHLDHFYALLAVMS 552
           +MKSLG++Y+ TSL IGNF+SSF+LS VS+IT ++G GWILNNLN S LD++Y   AV++
Sbjct: 488 SMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKRGRGWILNNLNESRLDYYYLFFAVLN 547

Query: 553 AVNFFVFLVISKWYVYRAEVSDSIKVLTDELKK 585
            VNF +FLV+ K+YVYRAEV+DS+ V   E+K+
Sbjct: 548 LVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of Spg031688 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 696.8 bits (1797), Expect = 1.5e-200
Identity = 341/566 (60.25%), Postives = 440/566 (77.74%), Query Frame = 0

Query: 13  DDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGT 72
           DD+TKDG+VDL+GN V RS+TG WKACSF++VYE+ ER  + GIS NL IY+TTKL+QGT
Sbjct: 8   DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTTKLHQGT 67

Query: 73  VTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKP 132
           V +SNNVTNW G  W+TPILGAY+ADAH GRY TF+ISSA+ L  M+LLTL+VS+P LKP
Sbjct: 68  VKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVSLPGLKP 127

Query: 133 PPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKL 192
           P C   N ENC+ AS +QLAVFFG+LY LA+ TGGTKPNIST+GADQFD+F  ++K  K 
Sbjct: 128 PKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPKDKIHKH 187

Query: 193 SFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLP 252
           SFFNWWMFSIF G  FA+T++VY+QDNVGW++GYG+ T+G+A +I IF+ GT  YRH+LP
Sbjct: 188 SFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRLYRHKLP 247

Query: 253 NGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI 312
            GSP T+MA VIVA+ R  R P+ +D    +EL    Y+    F I ST SLRFLN+A++
Sbjct: 248 MGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRFLNRASL 307

Query: 313 RRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDRSIGSH 372
           +  S+H WRLC++T+VEETKQML+M+P+L  TF+P+ +++Q  TLFIKQ TTLDR + ++
Sbjct: 308 KTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGTTLDRRLTNN 367

Query: 373 FKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHVLIMAV 432
           F IPPAS   F T SMLVSI+IYDR+FVK M+++T NPRGIT+LQR GIGMI H+LIM +
Sbjct: 368 FSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIGMILHILIMII 427

Query: 433 AFQVERHRLDVAKEKGLV-KNGGQLPLTIFILLPQFILTGIAEALLNIAITEFFYDQAPE 492
           A   ER+RL VA E GL  +    +PL+IF LLPQ++L G+A+A + IA  EFFYDQAPE
Sbjct: 428 ASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADAFIEIAKLEFFYDQAPE 487

Query: 493 NMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGNGWILNNLNASHLDHFYALLAVMS 552
           +MKSLG++YT TS+ +G F+SS +LS VS IT +QG GWI NNLN S LD++Y   AV++
Sbjct: 488 SMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKKQGRGWIQNNLNESRLDNYYMFFAVLN 547

Query: 553 AVNFFVFLVISKWYVYRAEVSDSIKV 578
            +NF +FLV+ ++Y YRA+V+ S  V
Sbjct: 548 LLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Spg031688 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 533.9 bits (1374), Expect = 1.7e-151
Identity = 275/558 (49.28%), Postives = 387/558 (69.35%), Query Frame = 0

Query: 15  FTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGTVT 74
           +T+DG+VDL+G PVL SKTG W+ACSF++ YE  ER  F GI+ NL  YLT +L++ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 75  ASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKPPP 134
           +  NV NW+GAVWITPI GAYIAD+++GR+ TF  SS + +  M LLT+AV++ SL+P  
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRP-- 126

Query: 135 CLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKLSF 194
                   C  AS LQ+  F+ SLY +A+  GGTKPNIST GADQFD + + EK  K+SF
Sbjct: 127 --TCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKVSF 186

Query: 195 FNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLPNG 254
           FNWWMFS F G LFA+  +VYIQ+N+GW LGYGIPT+G+ V++V+F  GTPFYRH++   
Sbjct: 187 FNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVIKT 246

Query: 255 SPITR-MANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAIR 314
             + + +  V +AA +N +L  P+D  EL+EL+  +Y  NG  ++  TP  RFL+KAAI+
Sbjct: 247 DNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAAIK 306

Query: 315 RDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDRSIGSHF 374
             S  P   C+VT+VE  K++L +I I L T +P+T+ +Q  TLF+KQ TTLDR IGS+F
Sbjct: 307 TSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGSNF 366

Query: 375 KIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHVLIMAVA 434
           +IP AS   FVT+SML+S+ +YD+ FV  M++ T NPRGIT+LQR G+G    ++ +A+A
Sbjct: 367 QIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIAIA 426

Query: 435 FQVERHRLDVAKEKGLVKNGGQLPLTIFILLPQFILTGIAEALLNIAITEFFYDQAPENM 494
             VE  R+ V KE  +      +P++IF LLPQ+ L GI +    I + EFFYDQ+PE M
Sbjct: 427 SAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQSPEEM 486

Query: 495 KSLGSTYTMTSLGIGNFLSSFILSKVSDITIR-QGNGWILNNLNASHLDHFYALLAVMSA 554
           +SLG+T+  + +G+GNFL+SF+++ +  IT +  G  WI NNLN S LD++Y  L V+S 
Sbjct: 487 QSLGTTFFTSGIGLGNFLNSFLVTMIDKITSKGGGKSWIGNNLNDSRLDYYYGFLVVISI 546

Query: 555 VNFFVFLVISKWYVYRAE 571
           VN  +F+  +  YVY+++
Sbjct: 547 VNMGLFVWAASKYVYKSD 557

BLAST of Spg031688 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 467.2 bits (1201), Expect = 1.9e-131
Identity = 251/567 (44.27%), Postives = 363/567 (64.02%), Query Frame = 0

Query: 13  DDFTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGT 72
           D +T+DG+VD+  NP  + KTG WKAC FI+  E  ER  + G+  NL  YL ++LNQG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 73  VTASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKP 132
            TA+NNVTNW+G  +ITP++GA+IADA+LGRY T      + ++ M+LLTL+ S+P LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 133 PPCLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKL 192
             C   N + C H +  Q AVFF +LY++AL TGG KP +S+ GADQFD+    EK  K 
Sbjct: 125 GNC---NADTC-HPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 184

Query: 193 SFFNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLP 252
           SFFNW+ FSI  G L A+T++V+IQ NVGW  G+G+PT+ + +A+  F  G+ FYR + P
Sbjct: 185 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 244

Query: 253 NGSPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI 312
            GSP+TR+  VIVAA R   + +P D   L E   +  +  G+ K+  T +L+F +KAA+
Sbjct: 245 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 304

Query: 313 RRDSS-------HPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTL 372
              S        +PWRLCSVTQVEE K ++ ++P+     +  T+ SQ  T+F+ Q  T+
Sbjct: 305 ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 364

Query: 373 DRSIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIF 432
           D+ +G +F+IP AS  +F T+S+L    +YD+  + + ++ T+N RG T LQR GIG++ 
Sbjct: 365 DQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVV 424

Query: 433 HVLIMAVAFQVERHRLDVAKEKGLVKNGGQLPLTIFILLPQFILTGIAEALLNIAITEFF 492
            +  M  A  +E  RLD  K      +  Q+ ++IF  +PQ++L G AE    I   EFF
Sbjct: 425 SIFAMITAGVLEVVRLDYVKTHN-AYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 484

Query: 493 YDQAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQGN-GWILNNLNASHLDHFY 552
           YDQAP+ M+SL S  ++T++ +GN+LS+ +++ V  IT + G  GWI +NLN  HLD+F+
Sbjct: 485 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFF 544

Query: 553 ALLAVMSAVNFFVFLVISKWYVYRAEV 572
            LLA +S +NF V+L ISK Y Y+  V
Sbjct: 545 YLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Spg031688 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 461.1 bits (1185), Expect = 1.4e-129
Identity = 249/559 (44.54%), Postives = 359/559 (64.22%), Query Frame = 0

Query: 15  FTKDGSVDLKGNPVLRSKTGCWKACSFIIVYELIERTMFVGISVNLFIYLTTKLNQGTVT 74
           + +DGSVD  GNP L+ KTG WKAC FI+  E  ER  + GI+ NL  YLTTKL+QG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 75  ASNNVTNWTGAVWITPILGAYIADAHLGRYRTFLISSALCLTAMSLLTLAVSIPSLKPPP 134
           A+ NVT W G  ++TP++GA +ADA+ GRY T    S +    MS LTL+ S+P+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 135 CLETNKENCKHASKLQLAVFFGSLYLLALATGGTKPNISTMGADQFDDFHLREKALKLSF 194
           C+    + C  A+  Q A+FFG LYL+AL TGG KP +S+ GADQFDD   RE+  K SF
Sbjct: 144 CI---GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 203

Query: 195 FNWWMFSIFTGILFASTIVVYIQDNVGWSLGYGIPTIGVAVAIVIFVAGTPFYRHRLPNG 254
           FNW+ FSI  G L +S+++V+IQ+N GW LG+GIPT+ + +AI  F  GTP YR + P G
Sbjct: 204 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 263

Query: 255 SPITRMANVIVAAARNWRLPLPNDPKELHELELEHYSKNGTFKIDSTPSLRFLNKAAI-- 314
           SPITR++ V+VA+ R   + +P D   L+E + ++ +  G+ KI+ T   ++L+KAA+  
Sbjct: 264 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 323

Query: 315 -----RRDSSHPWRLCSVTQVEETKQMLRMIPILLCTFLPNTIMSQSYTLFIKQCTTLDR 374
                  D S+ WRLC+VTQVEE K ++RM PI     + + + +Q  T+F++Q   ++ 
Sbjct: 324 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 383

Query: 375 SIGSHFKIPPASFYVFVTISMLVSIIIYDRIFVKIMKRVTKNPRGITMLQRTGIGMIFHV 434
            IGS F++PPA+   F T S+++ + +YDR  V + ++ T   +G T +QR GIG+   V
Sbjct: 384 KIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSV 443

Query: 435 LIMAVAFQVERHRLDVAKEKGLVKNGGQLPLTIFILLPQFILTGIAEALLNIAITEFFYD 494
           L MA A  VE  RL +A + GLV++G  +P+++   +PQ+ + G AE    I   EFFYD
Sbjct: 444 LCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYD 503

Query: 495 QAPENMKSLGSTYTMTSLGIGNFLSSFILSKVSDITIRQG-NGWILNNLNASHLDHFYAL 554
           Q+P+ M+SL S   + +  +GN+LSS IL+ V+  T R G  GWI +NLN+ HLD+F+ L
Sbjct: 504 QSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQEGWISDNLNSGHLDYFFWL 563

Query: 555 LAVMSAVNFFVFLVISKWY 566
           LA +S VN  V+   +  Y
Sbjct: 564 LAGLSLVNMAVYFFSAARY 578