Spg031577 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031577
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	enoyl-CoA delta isomerase 2, peroxisomal-like
Location	scaffold11: 40221334 .. 40222053 (+)
RNA-Seq Expression	Spg031577
Synteny	Spg031577

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCACAGTTGAGACGCGAGGCAATCTCTTCATTCTAACTCTGACCGGCAACGACGATCACCGCCTCGGCCCGTCCTTGATCAACTCCATCCTCAAAGCTCTTTCCAAGATCAAGGCCCAAGCTTTCCCTGGTTCAGTTCTCATCACAACTTCCCATGGCAAGTTCTTCTCTAACGGCTTCGATCTCCCCTGGGCCCTCGCCGCCGGCTCCGTTTCTGGCGCCCGTGATCGCCTGAACCACATAATCGATATCTTCAAGCCCGTTGTAGCAGAACTACTTTCCCTTCCTATGCCTACCATTGCTGCCGTTGCAGGACACGCCGCTGCCGCTGGTTTCACATTGGCGCTGAGCCACGACTATTTGATCATGAGGAGCGATCGGGGCGTTCTGTACATGCGTGAGTTGGATCTGGGTCTAACTCTGCCGGATTACTTTATGGCTCTGTTTAAGTCCAAGATGGGCTCGAGTTTGGCTATGCGCGACCTGATCCTGAAGGGGATGAAAGTGAGAGGTGAAGCGGCGTTGAAATTGGGCGTCGTGGAATCGGCGCACGACGGCGAGGACGGCGTCGTGAAGGCTGCAGTGCGGCTCGGGGAGCAGCTGGCGGCGAGAAATTGGGACGGCGAAGCATATGCTGAAATCAGGACGAGTTTGTATCCTGAGGTCTCTGTGGTGCTTGGATTACCGGGGAAGGCCATATCGATTTCCAAACTATGA

mRNA sequence

ATGTGCACAGTTGAGACGCGAGGCAATCTCTTCATTCTAACTCTGACCGGCAACGACGATCACCGCCTCGGCCCGTCCTTGATCAACTCCATCCTCAAAGCTCTTTCCAAGATCAAGGCCCAAGCTTTCCCTGGTTCAGTTCTCATCACAACTTCCCATGGCAAGTTCTTCTCTAACGGCTTCGATCTCCCCTGGGCCCTCGCCGCCGGCTCCGTTTCTGGCGCCCGTGATCGCCTGAACCACATAATCGATATCTTCAAGCCCGTTGTAGCAGAACTACTTTCCCTTCCTATGCCTACCATTGCTGCCGTTGCAGGACACGCCGCTGCCGCTGGTTTCACATTGGCGCTGAGCCACGACTATTTGATCATGAGGAGCGATCGGGGCGTTCTGTACATGCGTGAGTTGGATCTGGGTCTAACTCTGCCGGATTACTTTATGGCTCTGTTTAAGTCCAAGATGGGCTCGAGTTTGGCTATGCGCGACCTGATCCTGAAGGGGATGAAAGTGAGAGGTGAAGCGGCGTTGAAATTGGGCGTCGTGGAATCGGCGCACGACGGCGAGGACGGCGTCGTGAAGGCTGCAGTGCGGCTCGGGGAGCAGCTGGCGGCGAGAAATTGGGACGGCGAAGCATATGCTGAAATCAGGACGAGTTTGTATCCTGAGGTCTCTGTGGTGCTTGGATTACCGGGGAAGGCCATATCGATTTCCAAACTATGA

Coding sequence (CDS)

ATGTGCACAGTTGAGACGCGAGGCAATCTCTTCATTCTAACTCTGACCGGCAACGACGATCACCGCCTCGGCCCGTCCTTGATCAACTCCATCCTCAAAGCTCTTTCCAAGATCAAGGCCCAAGCTTTCCCTGGTTCAGTTCTCATCACAACTTCCCATGGCAAGTTCTTCTCTAACGGCTTCGATCTCCCCTGGGCCCTCGCCGCCGGCTCCGTTTCTGGCGCCCGTGATCGCCTGAACCACATAATCGATATCTTCAAGCCCGTTGTAGCAGAACTACTTTCCCTTCCTATGCCTACCATTGCTGCCGTTGCAGGACACGCCGCTGCCGCTGGTTTCACATTGGCGCTGAGCCACGACTATTTGATCATGAGGAGCGATCGGGGCGTTCTGTACATGCGTGAGTTGGATCTGGGTCTAACTCTGCCGGATTACTTTATGGCTCTGTTTAAGTCCAAGATGGGCTCGAGTTTGGCTATGCGCGACCTGATCCTGAAGGGGATGAAAGTGAGAGGTGAAGCGGCGTTGAAATTGGGCGTCGTGGAATCGGCGCACGACGGCGAGGACGGCGTCGTGAAGGCTGCAGTGCGGCTCGGGGAGCAGCTGGCGGCGAGAAATTGGGACGGCGAAGCATATGCTGAAATCAGGACGAGTTTGTATCCTGAGGTCTCTGTGGTGCTTGGATTACCGGGGAAGGCCATATCGATTTCCAAACTATGA

Protein sequence

MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNGFDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHDYLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGVVESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL

Homology

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BLAST of Spg031577 vs. NCBI nr
Match: XP_038877355.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 419.1 bits (1076), Expect = 2.6e-113
Identity = 212/239 (88.70%), Postives = 224/239 (93.72%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCTVE RGN FILTL GNDDHRLGPSLINSILKALS++KAQAFPGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEERGNFFILTLIGNDDHRLGPSLINSILKALSQVKAQAFPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWAL AGSVS ARDRLNHIIDIFKPVVAELLSLPMPTIAA+ GHAAAAGFTLALSHD
Sbjct: 61  FDLPWALTAGSVSAARDRLNHIIDIFKPVVAELLSLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSK+GSSLA+RDLIL+GMKV GEAA+++GV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAVRDLILRGMKVGGEAAVRMGV 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           VESAH G+D VV AAVRLGEQLA RNWDGE YA++R SLYPEVS VLGL GKAISISKL
Sbjct: 181 VESAHQGQDAVVNAAVRLGEQLAGRNWDGEVYAKVRRSLYPEVSEVLGLTGKAISISKL 239

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BLAST of Spg031577 vs. NCBI nr
Match: XP_022979202.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 414.1 bits (1063), Expect = 8.3e-112
Identity = 210/239 (87.87%), Postives = 223/239 (93.31%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCT+E RGN FILTLTG+DDHRLGPSLINSILKALS++KAQA PGSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWALAAGS+SGARDRLNHII IFKP+VA+LLSLPMPTIAA+AGHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALF SKMGSSLAMRDLIL+GMKVRGEAA+++GV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           VES H GEDGV  AAV+LGEQLAAR WDGE YAEIR SLYPEVS VLGL GKAISISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

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BLAST of Spg031577 vs. NCBI nr
Match: XP_004134079.1 (enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56881.1 hypothetical protein Csa_010206 [Cucumis sativus])

HSP 1 Score: 412.5 bits (1059), Expect = 2.4e-111
Identity = 208/239 (87.03%), Postives = 223/239 (93.31%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCTVE RGN FILTLTG+DDHRL PSLINSILKALS++KAQA P SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWAL+AGSVS AR RLNH+I+IFK VVAEL+SLPMPTIAA+ GHAAAAGFTLALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSK+GSSLAMRDLIL+GMKVRGEAA+++G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           V+SAHDGED VV AAVRLGEQLA RNWDGE YAEIR SLYPEVS VLGLPGK ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

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BLAST of Spg031577 vs. NCBI nr
Match: XP_023526416.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 412.1 bits (1058), Expect = 3.2e-111
Identity = 208/239 (87.03%), Postives = 224/239 (93.72%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCTVE RGN FILTLTG+DDHRLGPSLINS+LKALS++KAQA PGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWALAAGS+SGARDRL+HII IFKP+VA+LLSLPMPTIAA++GHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLSHIIQIFKPLVAQLLSLPMPTIAAISGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALFKSKMGSSLAMRDLIL+GMKVRGEAA+++GV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLAMRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           VES H GEDGV  AAV+LGEQLAAR WDGE YAEIR S+YPEVS VLGL GKAISISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWDGEVYAEIRKSVYPEVSEVLGLRGKAISISKL 239

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BLAST of Spg031577 vs. NCBI nr
Match: KAG6582371.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 407.9 bits (1047), Expect = 6.0e-110
Identity = 208/239 (87.03%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCTVE RGN FILTLTG+DDHRLGPSLINSILKALS++KAQA  GSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATSGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWALAAGS+SGARDRLNHII IFKP+VA+LLSLPMPTIAA+AGHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALFKSKMGSSLA RDLIL+GMKVRGEAA+++GV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           VES H GEDGV  AAV+LGEQLAAR W+GE YAEIR SLYPEVS VLGL GK ISISKL
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPISISKL 239

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BLAST of Spg031577 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.1e-77
Identity = 145/240 (60.42%), Postives = 188/240 (78.33%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCT+E RG+LF+LTLTG+ +HR  P  I +IL  L + K+Q+  GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDL WA  AGS +GA +RL+ +++ FKPVVA LL LPMPTIAA+ GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           Y+ MR DRGVLYM E+D+GL++PDYF AL ++K+G+S A R+L+L G K+RGE A+ LG+
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           V+S A+D E+GVV A+VRLGE+LAA+ W GE YA IR SLYPE+  +LGL  +  +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

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BLAST of Spg031577 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 6.7e-72
Identity = 138/240 (57.50%), Postives = 177/240 (73.75%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCT+E RG+LF+LTLTG D+HR  P  I S+L  L + K+Q+  GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDL WA +AG   GA  R++ ++  FKPV+A LL LPMPTIAA+ GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           Y+ MR DRGVLYM E+D+GL +PDYF AL  +K+GS +A R+L+L G K++GE A+ LG+
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           V+S AHD  +GVV+A V LGE LAA+ W+GE YA IR SLYPE+  ++ L    ++   L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNLATHNL 238

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BLAST of Spg031577 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 2.7e-52
Identity = 109/226 (48.23%), Postives = 156/226 (69.03%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQ-AFPGSVLITTSHGKFFSN 60
           MC++E R  LFIL LTG+ +HRL P+L++S+   +++I++  +F  SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSH 120
           G+DL  A +  S+S   D         + +VA+L+SLPMPTIAAV GHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLG 180
           DY++MR DRG LYM ELD+ L +P +FMA+ + K+GS  A RD++L   KV  +  +K+G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 VVESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSV 226
           +V+SA+      V+AA++LGE++  R  DG  Y ++R SL  EV +
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREVLI 220

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BLAST of Spg031577 vs. ExPASy Swiss-Prot
Match: A9MR28 (Carnitinyl-CoA dehydratase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) OX=41514 GN=caiD PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 4.9e-06
Identity = 53/158 (33.54%), Postives = 76/158 (48.10%), Query Frame = 0


Query: 48  LITTSHGKFFSNGFDLPWALAAGSVSGARDRLNHIIDIFKP----VVAELLSLPMPTIAA 107
           +IT +  KFFS G+DL  A A G    A          F P     + EL  L  P IAA
Sbjct: 51  IITGAGEKFFSAGWDLK-AAAEGEAPDAD---------FGPGGFAGLTELFDLNKPVIAA 110

Query: 108 VAGHAAAAGFTLALSHDYLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDL 167
           V G+A   GF LAL+ D++I  +D     + E  LG+ +PD    L   K+     + D+
Sbjct: 111 VNGYAFGGGFELALAADFIIC-ADHASFALPEAKLGI-VPDSGGVLRLPKILPPAIVNDM 170

Query: 168 ILKGMKVRGEAALKLGVVE---SAHDGEDGVVKAAVRL 199
           ++ G ++  E AL+ GVV    S H+  D   + A +L
Sbjct: 171 VMTGRRMTAEEALRWGVVNRVVSPHELLDSARELARQL 196

[Close]

BLAST of Spg031577 vs. ExPASy Swiss-Prot
Match: O07533 (Putative enoyl-CoA hydratase/isomerase YhaR OS=Bacillus subtilis (strain 168) OX=224308 GN=yhaR PE=3 SV=4)

HSP 1 Score: 51.6 bits (122), Expect = 1.4e-05
Identity = 43/168 (25.60%), Postives = 76/168 (45.24%), Query Frame = 0


Query: 21  HRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNGFDLPWALAAGSVSGARDRLN 80
           H L   +++ + +A+    A A    +++    GK FS G D+    +        D+  
Sbjct: 24  HALNEQMLSELKEAVE--MAAASEALIVLLRGSGKGFSAGGDIRMMTSEHD----PDQFK 83

Query: 81  HIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHDYLIMRSDRGVLYMRELDLGL 140
            ++D  + V   L  +   TIAA+ G AA  G +LAL  D +++     VL M  + +GL
Sbjct: 84  RLMDTIEAVTLNLYQMKKVTIAAIHGAAAGLGLSLALCAD-IVLAEKNAVLAMNFIGIGL 143

Query: 141 TLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGVVESAHDGE 189
                   L K ++G + A + LI  G K+    A  +G+++    G+
Sbjct: 144 VPDGGGHYLLKKRIGEAKA-KKLIWSGKKLSASEAADMGLLDGTFAGD 183

[Close]

BLAST of Spg031577 vs. ExPASy TrEMBL
Match: A0A6J1IN54 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111479008 PE=4 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 4.0e-112
Identity = 210/239 (87.87%), Postives = 223/239 (93.31%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCT+E RGN FILTLTG+DDHRLGPSLINSILKALS++KAQA PGSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRGNFFILTLTGDDDHRLGPSLINSILKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWALAAGS+SGARDRLNHII IFKP+VA+LLSLPMPTIAA+AGHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALF SKMGSSLAMRDLIL+GMKVRGEAA+++GV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFMSKMGSSLAMRDLILRGMKVRGEAAVRIGV 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           VES H GEDGV  AAV+LGEQLAAR WDGE YAEIR SLYPEVS VLGL GKAISISKL
Sbjct: 181 VESTHHGEDGVANAAVQLGEQLAARKWDGEVYAEIRKSLYPEVSEVLGLRGKAISISKL 239

[Close]

BLAST of Spg031577 vs. ExPASy TrEMBL
Match: A0A0A0L9X4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.2e-111
Identity = 208/239 (87.03%), Postives = 223/239 (93.31%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCTVE RGN FILTLTG+DDHRL PSLINSILKALS++KAQA P SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWAL+AGSVS AR RLNH+I+IFK VVAEL+SLPMPTIAA+ GHAAAAGFTLALSHD
Sbjct: 61  FDLPWALSAGSVSAARHRLNHLIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSK+GSSLAMRDLIL+GMKVRGEAA+++G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           V+SAHDGED VV AAVRLGEQLA RNWDGE YAEIR SLYPEVS VLGLPGK ISISKL
Sbjct: 181 VDSAHDGEDSVVNAAVRLGEQLAGRNWDGEVYAEIRKSLYPEVSEVLGLPGKPISISKL 239

[Close]

BLAST of Spg031577 vs. ExPASy TrEMBL
Match: A0A6J1E8J9 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431761 PE=4 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 6.4e-110
Identity = 206/239 (86.19%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCTVE RGN FILTLTG+DDHRLGPSLINS+LKALS++KAQA PGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLGPSLINSLLKALSQVKAQATPGSVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWALAAGS+SGARDRLNHII IFKP+VA+LLSLPMPTIAA+AGHAAAAGF LALSHD
Sbjct: 61  FDLPWALAAGSISGARDRLNHIIQIFKPLVAQLLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYF ALFKSKMGSSLA RDLIL+GMKVRGEAA+++GV
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFTALFKSKMGSSLATRDLILRGMKVRGEAAVRMGV 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           VES H GEDGV  AAV+LGEQLAAR W+GE YAEIR SLYPEVS VLGL GK I IS+L
Sbjct: 181 VESTHHGEDGVFNAAVQLGEQLAARKWNGEVYAEIRKSLYPEVSEVLGLRGKPIFISRL 239

[Close]

BLAST of Spg031577 vs. ExPASy TrEMBL
Match: A0A1S3AWB3 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483618 PE=4 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.1e-109
Identity = 206/239 (86.19%), Postives = 220/239 (92.05%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCTVE RGN FILTLTG+DDHRL PSLINSILKALS++KAQA P SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWALAAGSVS AR RLNHII+IFK VVAEL+SLPMPTIAA+ GHAAAAGFTLALSHD
Sbjct: 61  FDLPWALAAGSVSAARHRLNHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSK+GSSLAMRDLIL+GMKVRGEAA+++G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           V+SAH  E  VV AAVRLGEQL  RNWDGE YAEIR SLYPE+S VLGLPGKAISISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

[Close]

BLAST of Spg031577 vs. ExPASy TrEMBL
Match: A0A5D3CZF5 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001370 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.9e-109
Identity = 205/239 (85.77%), Postives = 220/239 (92.05%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCTVE RGN FILTLTG+DDHRL PSLINSILKALS++KAQA P SVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNFFILTLTGDDDHRLSPSLINSILKALSQVKAQASPASVLITTSHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDLPWALAAGSVS AR R+NHII+IFK VVAEL+SLPMPTIAA+ GHAAAAGFTLALSHD
Sbjct: 61  FDLPWALAAGSVSAARHRINHIIEIFKSVVAELISLPMPTIAAITGHAAAAGFTLALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSK+GSSLAMRDLIL+GMKVRGEAA+++G+
Sbjct: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKIGSSLAMRDLILRGMKVRGEAAVRMGI 180

Query: 181 VESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           V+SAH  E  VV AAVRLGEQL  RNWDGE YAEIR SLYPE+S VLGLPGKAISISKL
Sbjct: 181 VDSAHHSEVSVVNAAVRLGEQLVGRNWDGEVYAEIRKSLYPEISEVLGLPGKAISISKL 239

[Close]

BLAST of Spg031577 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 291.2 bits (744), Expect = 7.6e-79
Identity = 145/240 (60.42%), Postives = 188/240 (78.33%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCT+E RG+LF+LTLTG+ +HR  P  I +IL  L + K+Q+  GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDL WA  AGS +GA +RL+ +++ FKPVVA LL LPMPTIAA+ GHAAAAG  LALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           Y+ MR DRGVLYM E+D+GL++PDYF AL ++K+G+S A R+L+L G K+RGE A+ LG+
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           V+S A+D E+GVV A+VRLGE+LAA+ W GE YA IR SLYPE+  +LGL  +  +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of Spg031577 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 271.9 bits (694), Expect = 4.8e-73
Identity = 138/240 (57.50%), Postives = 177/240 (73.75%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQAFPGSVLITTSHGKFFSNG 60
           MCT+E RG+LF+LTLTG D+HR  P  I S+L  L + K+Q+  GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSHD 120
           FDL WA +AG   GA  R++ ++  FKPV+A LL LPMPTIAA+ GHAAA+G   ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLGV 180
           Y+ MR DRGVLYM E+D+GL +PDYF AL  +K+GS +A R+L+L G K++GE A+ LG+
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSVVLGLPGKAISISKL 240
           V+S AHD  +GVV+A V LGE LAA+ W+GE YA IR SLYPE+  ++ L    ++   L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDLTANNLATHNL 238

[Close]

BLAST of Spg031577 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 206.8 bits (525), Expect = 1.9e-53
Identity = 109/226 (48.23%), Postives = 156/226 (69.03%), Query Frame = 0


Query: 1   MCTVETRGNLFILTLTGNDDHRLGPSLINSILKALSKIKAQ-AFPGSVLITTSHGKFFSN 60
           MC++E R  LFIL LTG+ +HRL P+L++S+   +++I++  +F  SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLPWALAAGSVSGARDRLNHIIDIFKPVVAELLSLPMPTIAAVAGHAAAAGFTLALSH 120
           G+DL  A +  S+S   D         + +VA+L+SLPMPTIAAV GHA+AAG  LA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDRGVLYMRELDLGLTLPDYFMALFKSKMGSSLAMRDLILKGMKVRGEAALKLG 180
           DY++MR DRG LYM ELD+ L +P +FMA+ + K+GS  A RD++L   KV  +  +K+G
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 VVESAHDGEDGVVKAAVRLGEQLAARNWDGEAYAEIRTSLYPEVSV 226
           +V+SA+      V+AA++LGE++  R  DG  Y ++R SL  EV +
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESLLREVLI 220

The following BLAST results are available for this feature:

BLAST of Spg031577 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877355.1	2.6e-113	88.70	enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida]	[more]
XP_022979202.1	8.3e-112	87.87	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima]	[more]
XP_004134079.1	2.4e-111	87.03	enoyl-CoA delta isomerase 2, peroxisomal [Cucumis sativus] >KGN56881.1 hypotheti...	[more]
XP_023526416.1	3.2e-111	87.03	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita pepo subsp. pepo]	[more]
KAG6582371.1	6.0e-110	87.03	Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Spg031577 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23299	1.1e-77	60.42	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2...	[more]
O23300	6.7e-72	57.50	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1	[more]
O04469	2.7e-52	48.23	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1...	[more]
A9MR28	4.9e-06	33.54	Carnitinyl-CoA dehydratase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-...	[more]
O07533	1.4e-05	25.60	Putative enoyl-CoA hydratase/isomerase YhaR OS=Bacillus subtilis (strain 168) OX...	[more]

BLAST of Spg031577 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IN54	4.0e-112	87.87	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A0A0L9X4	1.2e-111	87.03	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141770 PE=4 SV=1	[more]
A0A6J1E8J9	6.4e-110	86.19	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A1S3AWB3	1.1e-109	86.19	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5D3CZF5	1.9e-109	85.77	Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119...	[more]

BLAST of Spg031577 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14430.1	7.6e-79	60.42	indole-3-butyric acid response 10	[more]
AT4G14440.1	4.8e-73	57.50	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	1.9e-53	48.23	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 25..204 e-value: 1.6E-19 score: 70.2
None	No IPR available	GENE3D	3.90.226.10		coord: 1..226 e-value: 2.4E-36 score: 127.1
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..232
None	No IPR available	PANTHER	PTHR11941:SF75	ENOYL-COA DELTA ISOMERASE 2, PEROXISOMAL	coord: 1..232
None	No IPR available	CDD	cd06558	crotonase-like	coord: 2..202 e-value: 1.00549E-20 score: 84.5344
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 3..208

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031577.1	Spg031577.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006635	fatty acid beta-oxidation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004165	dodecenoyl-CoA delta-isomerase activity
molecular_function	GO:0004300	enoyl-CoA hydratase activity
molecular_function	GO:0003824	catalytic activity