Spg031566 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031566
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionPMEI domain-containing protein
Locationscaffold11: 42011356 .. 42011907 (+)
RNA-Seq ExpressionSpg031566
SyntenySpg031566
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTATGCTTATACGCACCGTTTCATCGCGCTCGCATTTTCCTTCTTCGTCGCCTTCAATTTCGCAGCCGCCAACGACGTCGTTTCACGAACCTGCGAGATCTCCGCCGCGAGCGATCCGAACGTCCACCTCGACTTCTGTCTGCGTTCTCTCGCCGCCGCACCGGCGAGCGACACGGCCGACCTGTTCGGACTCGGTGCGATCTCGATCGAGTTGATCGGAAAGAACGCGACCAGTACGCGGCGGTACATCAAGCGCTTGTTGAAGAAGAAGAATCCGTCGCCGGACTCGTTCGTGAGGCCGCGGCTGTGCGATTGCCTAGAGCTCTACTCCGACGCCGGCGAGACGGTGCGCGAGGCGGCGGCGGAGTACGCTGCGAAGCGCTACGACGAGGCGAACGTGAAGTTGAGTTCGGTTATGGATGCGGTTACGACGTGCGAGGACGGATTCAAGGAAATGGACGGCGGAGTTTCGCCGTTGACGAAGAGGAACGGCGACGCGTTTGAGTTAACCGCCATCGCGCTCTGTATTCTCAATATGCGTCCTTGA

mRNA sequence

ATGATTTATGCTTATACGCACCGTTTCATCGCGCTCGCATTTTCCTTCTTCGTCGCCTTCAATTTCGCAGCCGCCAACGACGTCGTTTCACGAACCTGCGAGATCTCCGCCGCGAGCGATCCGAACGTCCACCTCGACTTCTGTCTGCGTTCTCTCGCCGCCGCACCGGCGAGCGACACGGCCGACCTGTTCGGACTCGGTGCGATCTCGATCGAGTTGATCGGAAAGAACGCGACCAGTACGCGGCGGTACATCAAGCGCTTGTTGAAGAAGAAGAATCCGTCGCCGGACTCGTTCGTGAGGCCGCGGCTGTGCGATTGCCTAGAGCTCTACTCCGACGCCGGCGAGACGGTGCGCGAGGCGGCGGCGGAGTACGCTGCGAAGCGCTACGACGAGGCGAACGTGAAGTTGAGTTCGGTTATGGATGCGGTTACGACGTGCGAGGACGGATTCAAGGAAATGGACGGCGGAGTTTCGCCGTTGACGAAGAGGAACGGCGACGCGTTTGAGTTAACCGCCATCGCGCTCTGTATTCTCAATATGCGTCCTTGA

Coding sequence (CDS)

ATGATTTATGCTTATACGCACCGTTTCATCGCGCTCGCATTTTCCTTCTTCGTCGCCTTCAATTTCGCAGCCGCCAACGACGTCGTTTCACGAACCTGCGAGATCTCCGCCGCGAGCGATCCGAACGTCCACCTCGACTTCTGTCTGCGTTCTCTCGCCGCCGCACCGGCGAGCGACACGGCCGACCTGTTCGGACTCGGTGCGATCTCGATCGAGTTGATCGGAAAGAACGCGACCAGTACGCGGCGGTACATCAAGCGCTTGTTGAAGAAGAAGAATCCGTCGCCGGACTCGTTCGTGAGGCCGCGGCTGTGCGATTGCCTAGAGCTCTACTCCGACGCCGGCGAGACGGTGCGCGAGGCGGCGGCGGAGTACGCTGCGAAGCGCTACGACGAGGCGAACGTGAAGTTGAGTTCGGTTATGGATGCGGTTACGACGTGCGAGGACGGATTCAAGGAAATGGACGGCGGAGTTTCGCCGTTGACGAAGAGGAACGGCGACGCGTTTGAGTTAACCGCCATCGCGCTCTGTATTCTCAATATGCGTCCTTGA

Protein sequence

MIYAYTHRFIALAFSFFVAFNFAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTADLFGLGAISIELIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNMRP
Homology
BLAST of Spg031566 vs. NCBI nr
Match: XP_023528439.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 287.3 bits (734), Expect = 9.0e-74
Identity = 153/185 (82.70%), Postives = 162/185 (87.57%), Query Frame = 0

Query: 5   YTHRFIALAFSFFVA--FNFAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTAD 64
           YTHRFIALA S FVA   N   ANDVV+RTCEISAA DPNVHLDFCLRSLAAAPASD AD
Sbjct: 5   YTHRFIALASSLFVALYLNSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPASDMAD 64

Query: 65  LFGLGAISIELIGKNATSTRRYIKRLL----KKKNPSPDSFVRPRLCDCLELYSDAGETV 124
           L+ LGAISIELIG+NATSTRRYI+RLL    KKKNPS DS+VRPRL DC ELYSD   TV
Sbjct: 65  LYELGAISIELIGRNATSTRRYIERLLKKNKKKKNPSLDSYVRPRLSDCRELYSDVKATV 124

Query: 125 REAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCI 184
           R+AAA+YA+KRY+E NVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRN DAFELTAIALCI
Sbjct: 125 RDAAADYASKRYEEVNVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCI 184

BLAST of Spg031566 vs. NCBI nr
Match: KAG6582515.1 (hypothetical protein SDJN03_22517, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018899.1 hypothetical protein SDJN02_20772, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 287.0 bits (733), Expect = 1.2e-73
Identity = 152/185 (82.16%), Postives = 162/185 (87.57%), Query Frame = 0

Query: 5   YTHRFIALAFSFFVA--FNFAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTAD 64
           YTHRFIALA S FVA   N   ANDVV+RTCEISAA DPNVHLDFCLRSLAAAPASDTAD
Sbjct: 5   YTHRFIALASSLFVALYLNSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPASDTAD 64

Query: 65  LFGLGAISIELIGKNATSTRRYIKRLL----KKKNPSPDSFVRPRLCDCLELYSDAGETV 124
           L+ LGAISIELIG+NATSTRRYI+RLL    KKKNPSPDS++RP L DC ELYSD   TV
Sbjct: 65  LYELGAISIELIGRNATSTRRYIERLLKKNKKKKNPSPDSYLRPPLNDCRELYSDVRVTV 124

Query: 125 REAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCI 184
           R+AAA+YA+KRY+E NVKLSSVMDA TTCEDGFKEMDGGVSPLTKRN DAFELTAIALCI
Sbjct: 125 RDAAADYASKRYEEVNVKLSSVMDAATTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCI 184

BLAST of Spg031566 vs. NCBI nr
Match: XP_022924291.1 (putative invertase inhibitor [Cucurbita moschata])

HSP 1 Score: 286.2 bits (731), Expect = 2.0e-73
Identity = 151/185 (81.62%), Postives = 163/185 (88.11%), Query Frame = 0

Query: 5   YTHRFIALAFSFFVAFNFAA--ANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTAD 64
           YTHRFIALA S FVA    +  ANDVV+RTCEISAA DPNVHLDFCLRSLAAAPASDTAD
Sbjct: 5   YTHRFIALASSLFVALYLTSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPASDTAD 64

Query: 65  LFGLGAISIELIGKNATSTRRYIKRLL----KKKNPSPDSFVRPRLCDCLELYSDAGETV 124
           L+ LGAISIELIG+NATSTRRYI+RLL    KKKNPSPDS++RP L DC ELYSD   TV
Sbjct: 65  LYELGAISIELIGRNATSTRRYIERLLKKKKKKKNPSPDSYLRPPLNDCRELYSDVRVTV 124

Query: 125 REAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCI 184
           R+AAA+YA+KRY+E NVKLSS+MDAVTTCEDGFKEMDGGVSPLTKRN DAFELTAIALCI
Sbjct: 125 RDAAADYASKRYEEVNVKLSSIMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCI 184

BLAST of Spg031566 vs. NCBI nr
Match: XP_038905481.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 285.0 bits (728), Expect = 4.5e-73
Identity = 149/186 (80.11%), Postives = 164/186 (88.17%), Query Frame = 0

Query: 1   MIYAYTHRFIALAFSFFVAFNF--AAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPAS 60
           M + YTHRFIALA+SF +AF+F   A+ND+V RTCEISAASDPNV LDFCLRSLAA PAS
Sbjct: 1   MSFTYTHRFIALAYSFLLAFSFQSTASNDIVLRTCEISAASDPNVRLDFCLRSLAADPAS 60

Query: 61  DTADLFGLGAISIELIGKNATSTRRYIKRLLKKKNP-SPDSFVRPRLCDCLELYSDAGET 120
           DTADL+ LGAISIELIG+NATSTRRYI RLLKKK   SPDS+VRPRL DC ELYSDA ET
Sbjct: 61  DTADLYELGAISIELIGRNATSTRRYIDRLLKKKKKLSPDSYVRPRLSDCKELYSDAVET 120

Query: 121 VREAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALC 180
           V +++AEYA KRY+E NVKLSSVMDAVTTCEDGFKEM+GG+SPLTKRNGD FELTAIALC
Sbjct: 121 VGDSSAEYARKRYEEVNVKLSSVMDAVTTCEDGFKEMEGGISPLTKRNGDVFELTAIALC 180

Query: 181 ILNMRP 184
           ILNM P
Sbjct: 181 ILNMHP 186

BLAST of Spg031566 vs. NCBI nr
Match: XP_008438273.1 (PREDICTED: putative invertase inhibitor [Cucumis melo])

HSP 1 Score: 270.8 bits (691), Expect = 8.7e-69
Identity = 144/187 (77.01%), Postives = 157/187 (83.96%), Query Frame = 0

Query: 1   MIYAYTHRFIALAFSFFVAFNF--AAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPAS 60
           M + YTHR+IALAFSF V  NF   +AND+VSRTCE SAA DPNV LDFCLRSLAAAP S
Sbjct: 1   MSFTYTHRYIALAFSFVVLLNFEPISANDIVSRTCETSAARDPNVRLDFCLRSLAAAPGS 60

Query: 61  DTADLFGLGAISIELIGKNATSTRRYIKRLLK--KKNPSPDSFVRPRLCDCLELYSDAGE 120
           DTADL+ LGAISI LIG+NATST+RYI+RLLK  KK  S DS++RPRL DC ELYSDA E
Sbjct: 61  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 120

Query: 121 TVREAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIAL 180
           TV EAAAEY  KRYDE NVKLSSVMDAVTTCEDGFKEM+  VSPLTKRNGD FEL AIAL
Sbjct: 121 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 180

Query: 181 CILNMRP 184
           CIL++RP
Sbjct: 181 CILDLRP 187

BLAST of Spg031566 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 4.9e-30
Identity = 74/168 (44.05%), Postives = 104/168 (61.90%), Query Frame = 0

Query: 14  FSFFVAFNFAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTADLFGLGAISIEL 73
           F+  +      + D+V  TC+  A   PNV+ DFC++SL A P S TADL GLG IS  L
Sbjct: 12  FNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVISANL 71

Query: 74  IGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEYAAKRYDEA 133
             ++ +  + +I R+LK K    D  ++  L DC+ LY+DA  +V+EA A++ +K Y  A
Sbjct: 72  AIQHGSKIQTFIGRILKSK---VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYASA 131

Query: 134 NVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNM 182
           NVK+S+ +D   TCEDGFKE  G VSP+TK N D  +LTAI+L I  +
Sbjct: 132 NVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAITKL 176

BLAST of Spg031566 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 8.3e-30
Identity = 74/170 (43.53%), Postives = 104/170 (61.18%), Query Frame = 0

Query: 12  LAFSFFVAFNFAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTADLFGLGAISI 71
           +  SF +   F  + D+V  TC+  A   PNV+ DFC++SL A P S +ADL GLG IS 
Sbjct: 1   MKLSFSLCIFFLISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISA 60

Query: 72  ELIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEYAAKRYD 131
            L  +  +  + +I R+LK K    D  ++  L DC+ LY+DA  +V+EA A++ +K Y 
Sbjct: 61  NLAIQQGSKIQTFIGRILKSK---VDPALKKYLNDCVGLYADAKSSVQEAIADFKSKDYA 120

Query: 132 EANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNM 182
            ANVK+S+ +D   TCEDGFKE  G  SP+TK N D  +LTAI+L I  +
Sbjct: 121 SANVKMSAALDDSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAITKL 167

BLAST of Spg031566 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.3e-11
Identity = 53/169 (31.36%), Postives = 78/169 (46.15%), Query Frame = 0

Query: 14  FSFFVAFNFAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTA-DLFGLGAISIE 73
           FS F+   FA A  ++  +C+ + A DP +  DFC+ SL   P S  A  L  L   S +
Sbjct: 10  FSLFLN-GFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLASTK 69

Query: 74  LIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEYAAKRYDE 133
                 T+ +  + + LK +       +   L  CL  Y+DA + +  A A   ++ Y  
Sbjct: 70  TAAAKITNLKGIVAQDLKDQRYQD---IVEDLKLCLGFYNDANDDLTTALANIKSRDYQG 129

Query: 134 ANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNM 182
           AN+ LS+ +D    CED FKE     SP+T  N   F+   I L   NM
Sbjct: 130 ANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

BLAST of Spg031566 vs. ExPASy TrEMBL
Match: A0A6J1E955 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111431821 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 9.7e-74
Identity = 151/185 (81.62%), Postives = 163/185 (88.11%), Query Frame = 0

Query: 5   YTHRFIALAFSFFVAFNFAA--ANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTAD 64
           YTHRFIALA S FVA    +  ANDVV+RTCEISAA DPNVHLDFCLRSLAAAPASDTAD
Sbjct: 5   YTHRFIALASSLFVALYLTSTVANDVVARTCEISAAKDPNVHLDFCLRSLAAAPASDTAD 64

Query: 65  LFGLGAISIELIGKNATSTRRYIKRLL----KKKNPSPDSFVRPRLCDCLELYSDAGETV 124
           L+ LGAISIELIG+NATSTRRYI+RLL    KKKNPSPDS++RP L DC ELYSD   TV
Sbjct: 65  LYELGAISIELIGRNATSTRRYIERLLKKKKKKKNPSPDSYLRPPLNDCRELYSDVRVTV 124

Query: 125 REAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCI 184
           R+AAA+YA+KRY+E NVKLSS+MDAVTTCEDGFKEMDGGVSPLTKRN DAFELTAIALCI
Sbjct: 125 RDAAADYASKRYEEVNVKLSSIMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCI 184

BLAST of Spg031566 vs. ExPASy TrEMBL
Match: A0A1S3AVZ7 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103483434 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 4.2e-69
Identity = 144/187 (77.01%), Postives = 157/187 (83.96%), Query Frame = 0

Query: 1   MIYAYTHRFIALAFSFFVAFNF--AAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPAS 60
           M + YTHR+IALAFSF V  NF   +AND+VSRTCE SAA DPNV LDFCLRSLAAAP S
Sbjct: 1   MSFTYTHRYIALAFSFVVLLNFEPISANDIVSRTCETSAARDPNVRLDFCLRSLAAAPGS 60

Query: 61  DTADLFGLGAISIELIGKNATSTRRYIKRLLK--KKNPSPDSFVRPRLCDCLELYSDAGE 120
           DTADL+ LGAISI LIG+NATST+RYI+RLLK  KK  S DS++RPRL DC ELYSDA E
Sbjct: 61  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 120

Query: 121 TVREAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIAL 180
           TV EAAAEY  KRYDE NVKLSSVMDAVTTCEDGFKEM+  VSPLTKRNGD FEL AIAL
Sbjct: 121 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 180

Query: 181 CILNMRP 184
           CIL++RP
Sbjct: 181 CILDLRP 187

BLAST of Spg031566 vs. ExPASy TrEMBL
Match: A0A5A7U1K7 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003190 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.6e-68
Identity = 144/187 (77.01%), Postives = 156/187 (83.42%), Query Frame = 0

Query: 1   MIYAYTHRFIALAFSFFVAFNF--AAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPAS 60
           M + YTHR IALAFSF V  NF   +AND+VSRTCE SAA DPNV LDFCLRSLAAAP S
Sbjct: 1   MSFTYTHRCIALAFSFVVLLNFEPISANDIVSRTCETSAARDPNVRLDFCLRSLAAAPGS 60

Query: 61  DTADLFGLGAISIELIGKNATSTRRYIKRLLK--KKNPSPDSFVRPRLCDCLELYSDAGE 120
           DTADL+ LGAISI LIG+NATST+RYI+RLLK  KK  S DS++RPRL DC ELYSDA E
Sbjct: 61  DTADLYELGAISIRLIGRNATSTQRYIERLLKNEKKKSSSDSYIRPRLSDCEELYSDAVE 120

Query: 121 TVREAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIAL 180
           TV EAAAEY  KRYDE NVKLSSVMDAVTTCEDGFKEM+  VSPLTKRNGD FEL AIAL
Sbjct: 121 TVGEAAAEYGRKRYDEVNVKLSSVMDAVTTCEDGFKEMESRVSPLTKRNGDVFELAAIAL 180

Query: 181 CILNMRP 184
           CIL++RP
Sbjct: 181 CILDLRP 187

BLAST of Spg031566 vs. ExPASy TrEMBL
Match: A0A6J1ITK8 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111479799 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 3.6e-68
Identity = 142/173 (82.08%), Postives = 151/173 (87.28%), Query Frame = 0

Query: 17  FVA--FNFAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTADLFGLGAISIELI 76
           FVA   N  AANDVV+R CEI AA DPNVHLDFCLRSLAAAPASD ADL+ LGAISIELI
Sbjct: 15  FVALYLNSTAANDVVARNCEIFAAKDPNVHLDFCLRSLAAAPASDMADLYELGAISIELI 74

Query: 77  GKNATSTRRYIKRLL----KKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEYAAKRY 136
           G+NATSTRRYI+RLL    KKKNPS DS+VRPRL DC ELYSD   TVR+AAA+YA+KRY
Sbjct: 75  GRNATSTRRYIERLLKKNKKKKNPSLDSYVRPRLSDCRELYSDVKATVRDAAADYASKRY 134

Query: 137 DEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNMRP 184
           +E NVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRN DAFELTAIALCILNMRP
Sbjct: 135 EEVNVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNDDAFELTAIALCILNMRP 187

BLAST of Spg031566 vs. ExPASy TrEMBL
Match: A0A0A0L7T4 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G128870 PE=4 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.4e-64
Identity = 135/185 (72.97%), Postives = 150/185 (81.08%), Query Frame = 0

Query: 1   MIYAYTHRFIALAFSFFVAFNF--AAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPAS 60
           M   Y HRFIALA SFFV FN    +AND +SR CE+SAASDPNV LDFCL+SLAAAP S
Sbjct: 1   MSITYMHRFIALALSFFVLFNLRSISANDTLSRACELSAASDPNVRLDFCLQSLAAAPGS 60

Query: 61  DTADLFGLGAISIELIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETV 120
           DTADL+ LGA+SI+LI  NATSTRRYI+RLLK +  SPD +VRPRL DC ELY DA + V
Sbjct: 61  DTADLYELGALSIKLIAWNATSTRRYIERLLKNEKKSPDPYVRPRLSDCEELYIDAIKAV 120

Query: 121 REAAAEYAAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCI 180
            +AA EY   RY+E NVKLSSVMDAVTTCEDGFKEM+G VSPLTKRNGD FELTAIAL I
Sbjct: 121 GDAAFEYGRNRYEEVNVKLSSVMDAVTTCEDGFKEMEGRVSPLTKRNGDVFELTAIALSI 180

Query: 181 LNMRP 184
           LN+RP
Sbjct: 181 LNLRP 185

BLAST of Spg031566 vs. TAIR 10
Match: AT5G38610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 84.3 bits (207), Expect = 1.1e-16
Identity = 60/183 (32.79%), Postives = 89/183 (48.63%), Query Frame = 0

Query: 12  LAFSFFVAFNFAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPAS--DTADLFGLGAI 71
           + F FF+     + +D++ +TC+  AA       +FC+ SL  +P +     +L  L  +
Sbjct: 9   IIFLFFLVSQVKSTSDMIDQTCKSCAAKSTIFDYNFCVSSLNNSPIALPSPTNLSSLALV 68

Query: 72  SIELIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEY-AAK 131
            +     NAT+T   I++LL   +    SF    L DCLELY DA + + EA   +   K
Sbjct: 69  PMLQALDNATATASTIQQLLISDDDG--SFRSACLRDCLELYEDATDRLEEAVRVFITRK 128

Query: 132 RYDEANVKLSSVMDAVTTCEDGFKEMDGG----------VSPLTKRNGDAFELTAIALCI 182
                NV +S+ M++  TCE+GF+E D G           SP+   N   FE   IALCI
Sbjct: 129 ELGTVNVMVSAAMESAVTCENGFRERDDGDGGGGGVTTWTSPIGDENHKLFEFGQIALCI 188

BLAST of Spg031566 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 9.1e-16
Identity = 56/176 (31.82%), Postives = 89/176 (50.57%), Query Frame = 0

Query: 8   RFIALAFSFFVAFN-FAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTA-DLFG 67
           +F+    +FFV  N  A    ++  +C+ + A+ P    + C+ SL   P + TA DL G
Sbjct: 2   KFLLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAG 61

Query: 68  LGAISIELIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEY 127
           L   S +     AT+ +  + +++K K    +      L DCL+LY+DA  ++ EA A  
Sbjct: 62  LVMASTKNAVTKATTLKGTVDKIIKGK--KVNKMTAMPLRDCLQLYTDAIGSLNEALAGV 121

Query: 128 AAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNM 182
            ++ Y      LS+ MD  +TCE GFKE     SP+TK N + +++  I L   NM
Sbjct: 122 KSRNYPTVKTVLSAAMDTPSTCETGFKERK-APSPVTKENDNLYQMILIPLAFTNM 174

BLAST of Spg031566 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.0e-14
Identity = 55/157 (35.03%), Postives = 83/157 (52.87%), Query Frame = 0

Query: 16  FFVAFN-FAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTA-DLFGLGAISIEL 75
           FF+  N F  A  ++   C+ +A  +P +H +FC++SL   P S TA  L  L  +S + 
Sbjct: 10  FFLLLNAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLVMLSTKN 69

Query: 76  IGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEYAAKRYDEA 135
                TS +  + ++LK+     + +    L DCLELYSDA  +++EA     ++ Y  A
Sbjct: 70  AVSKTTSMKGIVDKILKENR--FEMYSEKPLRDCLELYSDATNSLKEALTIIKSRDYKTA 129

Query: 136 NVKLSSVMDAVTTCEDGFKEMDGGV-SPLTKRNGDAF 170
           NV +S+ M A  +CE GFKE    + SP TK N   F
Sbjct: 130 NVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

BLAST of Spg031566 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 3.8e-14
Identity = 55/177 (31.07%), Postives = 90/177 (50.85%), Query Frame = 0

Query: 8   RFIALAFSFFVAFN-FAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTA-DLFG 67
           +F      FF+  N FA A  ++  +C+ +   DP +  DFC++SL   P S TA  L G
Sbjct: 2   KFSLYLVMFFLLLNCFATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEG 61

Query: 68  LGAISIELIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEY 127
           L  +S +      TS +  + ++LK+     +   RP L DCLELY++A +++ ++    
Sbjct: 62  LVFVSTKNAVSKTTSLKGMVDKILKEDKYEVE---RP-LLDCLELYTEAIDSLNQSLDTV 121

Query: 128 AAKRYDEANVKLSSVMDAVTTCEDGFKEMDGGV-SPLTKRNGDAFELTAIALCILNM 182
            ++ Y  A + +S+ MDA  +CE  F +    V SP TK N   F +  I +   +M
Sbjct: 122 KSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFTSM 174

BLAST of Spg031566 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.6e-12
Identity = 53/169 (31.36%), Postives = 78/169 (46.15%), Query Frame = 0

Query: 14  FSFFVAFNFAAANDVVSRTCEISAASDPNVHLDFCLRSLAAAPASDTA-DLFGLGAISIE 73
           FS F+   FA A  ++  +C+ + A DP +  DFC+ SL   P S  A  L  L   S +
Sbjct: 10  FSLFLN-GFATAQTLIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLASTK 69

Query: 74  LIGKNATSTRRYIKRLLKKKNPSPDSFVRPRLCDCLELYSDAGETVREAAAEYAAKRYDE 133
                 T+ +  + + LK +       +   L  CL  Y+DA + +  A A   ++ Y  
Sbjct: 70  TAAAKITNLKGIVAQDLKDQRYQD---IVEDLKLCLGFYNDANDDLTTALANIKSRDYQG 129

Query: 134 ANVKLSSVMDAVTTCEDGFKEMDGGVSPLTKRNGDAFELTAIALCILNM 182
           AN+ LS+ +D    CED FKE     SP+T  N   F+   I L   NM
Sbjct: 130 ANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNM 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023528439.19.0e-7482.70putative invertase inhibitor [Cucurbita pepo subsp. pepo][more]
KAG6582515.11.2e-7382.16hypothetical protein SDJN03_22517, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022924291.12.0e-7381.62putative invertase inhibitor [Cucurbita moschata][more]
XP_038905481.14.5e-7380.11putative invertase inhibitor [Benincasa hispida][more]
XP_008438273.18.7e-6977.01PREDICTED: putative invertase inhibitor [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q8GT414.9e-3044.05Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1[more]
A9YUH48.3e-3043.53Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1[more]
Q9FJR52.3e-1131.36Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E9559.7e-7481.62putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111431821 PE=4 ... [more]
A0A1S3AVZ74.2e-6977.01putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103483434 PE=4 SV=1[more]
A0A5A7U1K71.6e-6877.01Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A6J1ITK83.6e-6882.08putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111479799 PE=4 SV... [more]
A0A0A0L7T42.4e-6472.97PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G128870 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT5G38610.11.1e-1632.79Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46940.19.1e-1631.82Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46970.11.0e-1435.03Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46930.13.8e-1431.07Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46960.11.6e-1231.36Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 24..178
e-value: 1.4E-24
score: 97.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 9..181
e-value: 2.9E-34
score: 115.9
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 29..178
e-value: 4.3E-26
score: 92.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 23..181
e-value: 5.0E-35
score: 122.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 27..181
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 15..180
NoneNo IPR availablePANTHERPTHR31080:SF226PECTINESTERASE INHIBITOR DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 15..180
IPR034088Pla a 1-likeCDDcd15795PMEI-Pla_a_1_likecoord: 33..181
e-value: 1.11981E-46
score: 148.282

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031566.1Spg031566.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0004857 enzyme inhibitor activity