Spg031521 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031521
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Cytochrome P450
Location	scaffold11: 42890679 .. 42895430 (-)
RNA-Seq Expression	Spg031521
Synteny	Spg031521

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GACAATATGTGGAGATGGAGGTTCAAACCACAGACCTTGTGGTTATCAGTACAACACTAAGCCAGTAGAGCTATACTCCTATTGACATTTTTTAAGAAAAGTTTATCCATAAATTATGTCAAAGACTAATTTAAGATTTATTTGTAATTAGTCAGCATTGTTTTATTACTCTCTAAAATGTTGACCAAACAAATAATACTACCATTTGGAAAAAAATTAAAAGCCCGCTTTTTATCATCAATAATTACTTTTAAAAGTTGTCAACACACCTAAATCTCTGTGCTATTGATTACTGCAGAAACGTACTTCAATTTTCCTTATTATATTATATTAAGCGGCCTAACACTGAAGCATCCAGAGCTGAATTATCTGTTCATCTATCGCCATGATCGCTGTGAAATTTCTCTCAAACCCCATTTCAGTTGCAGCTCTATTGCTACTGATTTCCATTGTTGTAAGGCAGATTCTCAATGGAAAATCGAGGAGAAAGAAATATCATCCAATTGCAGGAACTGTATTTCACCAACTCCTCAATTTCCATCGATTGCATGATTATATGACTGATCTTGCTAGAAAGTATAAAACATATCGGCTTCTTGGTCCTTTCAGAAATGAGATTTACACTTCAGACCCACCAAATGTTGAGTATATACTCAGAACAAACTTCGAGAATTTTGGAAAGGTATGAGGTTTTCTTTTTCTTCCTTTTTCGGGTTGTAAAAGTTGAAATACTGTATGGATATTGGATTTACCATGGAATTGAGTTAAAACTGCAAACATGGGAGGGTGCATTTACCATGAAATTTGGGGTTCTCCATCCTAGTGATTGCATAAAATTTACTTGATTTTTGAATATTTCCGTGAACTCAAGTTATCTGAAAAGTTTGTGTTCTTCCATATGAAAATTAAGGTTTAGGTTTCCGAAATTAGTCAACAAACAACTTGAAAGCTCAAATGCTCAAGCGGGTCGATTATAGGACCTATTTGGAATGACTTTCTAAATGCTTAAAAAAGTGTTTTAAGTACTCAGAAAGTTGTTTTGAAAAGACCCTTAATCTTTTATACATATTCTTAATACGGGATTCTCTTGGAATGGTTCACTTTTCAATGTACTGGATTAGTGGATGTACCGGAACCGGATTGGTTAGAGTTTGTGTGGTATTTTATTTGGTATGATTGATGCTTCAAAAATGATGTTCTTGAAGGGCCATGATTTTGACTGAGTTTTGCACAATGTTCACTGTTCAGGGGTCACATAACCACAGAATTCTCAAGGATCTTCTTGGTGATGGAATTTTCACCGTTGATGGTGAGAAGTGGCGTCAGCAGAGGAAGATTTCAAGCTATGAGTTCTCTACAAAAGTTCTGAGGGATTTTAGCAGTGTCATATTCCAGAAATCTGCTGTTAAACTAGTTCATATCGTGTCCGAAGCTGCTATTTCAAATCGAGTAATTGATATTCAAGTAAGTTTCAACGATGCAGATGGCGGTAATGATTTCAAACACAATTTTTCCTTTCGGAAACCAGTAAACTCATTCAATTACTTATTTGCAGGATCTCTTTATGAAAACAACTCTAGATTCAATTTTTCATGTATCATTTGGAATTGATCTGGATAGCTTGCATGGATCAAATGAAGAAATAAACAGGTTTTGCTCTGCTTTTGAAGCATCAAGTACAATGACACTCTGGCGATATGTTGATGTAATCTGGCCAATCAAGAAGATTCTGAATATTGGGTCTGAAGCTACCTTGAAAAAAAACATGAAAATTGTTGATGAGTACATATATAAGCTCATTCACAACGCACTCGAACAGCGACAGAAGTTAGGCGAGGACACTTTGGTAGATGAATTTGTATCTTTGTTTTGCTTTAAACTTATTATATATGGAGCCTGATATTCTACTTTGGTATGTGAATTTAGATGACAAAAGATAACATAGTGTCGAGGTTTCTGCAAGCGGAAAAGACCAACCCGACGTATCTAAGAGATATTATTCTTAATTTTGTCATAGCTGGAAAGGACACAACAGCAGTTACACTTGCTTGGTTCATGTACGTGCTTTGCAAGCATCCTGACGTGCAGGAAAGAGTTGCTAGAGAGATCAAGGAAGTGACAAATGTTGAAGATGTTAACAATTTTGCAGAGTTTGCTGCAAGATTGACTGAAGAAGCTCTAGAGAAGATGCAGTTTCTCACTGCAACTATTAATGAAACTCTTAGACTCTATCCTGCTGTTCCAGTGGTATGGCATTTCCTCTTGAAATCTCAACATAGTTATTGCTAAGTATTTAAAATAGTTCTAAAATAATTGCACTGGTTAAACAGTTTACTTCTTCAGATAATGTCTTGGCATTTAGCAATTGATTTTCTCAACTACACCTTGAATAAATCTTGAGAGTAGTAAAAGCACTTAGTTAAGCTTCCCCTCACTGTCTTTATGTGTTTTTTTGAATTCTCATAGCCACTTTTAATTATCTGTGGAAATGCTATAAAAGTTTTGAAACAAACTTTTAATGGACTAAAAAAAATATAAGATCACCATTGAACTCAAAAGCTTAAGTAAATAGGTTGTGATAAAGTTTCACTTGTGGGTTTGAAAAATTAGCAAGAGTACAAGTTCTTTTTGTCTCAATACTATATTAAATCACCCTTTAACCTCGGAATGTTTGAAATCACTTTCGTCATGTCATTTTTAGTGAAAAAGAGCATTAAGTGGTTTTGGTCATTCTAAAATCATTCCCAAACATGCTCTAAAATAAAGTTTAAGTTAATGGGTTAAACATAAACTTCTTACCCTTTCACAAATCATGTTAAACTCGCTCTCGTAGCTTGTAAGTTATGGGCAAGTTTCATAATTTCTCGGTTGAATGAATCGACTTACTGTGTTAAGTTTTGAATTGCAGGATGCAAAGATTTGCTTTTCTGACGATACATTGCCAGATGGATTCAGTGTGAGAAAAGGAGATATGGTGTCATACCAGCCATATGCAATGGGTAGAATGAAATTCATATGGGGTGAAGATGCCGAGGAATTCCGACCAGAGAGATGGTTTGATGAAGATGGAAATTTCCAGCCTCAGAGCCCTTTCAAATTTACAGCCTTTCAGGTATGTGAGCAAAACATTAGCATATAACTGTCACAGTTCAAAATTGTTTGCATTGGAGCAAGTGCATAATATTCTTTAGTAGATATTTGGTTTAAAATTTATTTCATCTTGGCCCCTGCAATTTCAAATGTTTTGTTTCGGTTTCTAAATTCTGCATTAGAAAAATTATTGTTTTCGATCTTATTTTGGTCCTTTTTTCACAATGGTTTCTTCTTGGTCCATTTTTTTTTAGTTCAATAGATGTGAAATCGGATTGACTTCTTTTTGGTCTTTAAAATTTCAAATATTATGTTTTAGTTTTGAATTTTATACAAAACTATTGTAGTTTTTGTCGTTAATTTTTAAAAAATAATTTAACAAAAACTTGGCATGTGAAAAACCACATTTTTGTTAATTTACTTCCTGACTTGATATCTAAATAGACAAGTATTTGTTGTAGATCTAAAGTTGAGTTTCAAGACCTTTTTTTTTTTTTTTTTTTTTTTGAAGAAAGACAAACTTGGTTTTTTGTTAAATGATTAATGAAAATTTATTTATAAAGATTATAATAAATTTTTATACGAAATTAATTTAGTTGACGTGCTTTAATTTTTATTGTTAATTTGAAGTTTTTTTTTATTGAAATGAATGAAATATTAATTATTTCAAAAAATAAAATAAAATATTAATGATAATTTTAATTCAAGAAAATACTCGATGTGAATATATTTCAAAATTTATAGTGGGAATGTTAGACCACTCTTTTATGAAAAAGCTACATAAACTAACAGTTGGACTAATTTTAAGGTTTATTGAAAGTTACTAAATTTGGACGTTTAAAGATACTAAGACTGAAATAGAACAAATCTCAAAGTACAAAGATTAAAATGATATCTTAACCTATAAATATTTTATTCAAAGTTGAAATACTAAAATAAATCCTAGACCAAGATAAGAATAAGTATGATTTAGCCCTAGATATATTCTCTAAAATGGGCTTGAAGAGGGAGAAATGAACGAAAATTATGTTTTTCTTTTTTTTTTTTTCCTAATGAATTAGCTTGTGATTATGAGGCTCTCAATTAAGACATCTGATGATTCACAGGCAGGGCCACGCATTTGTCTGGGAAGAGACTTTGCTTACAGGCAGATGAAGATCCTCTCATCTCTATTATTGGTCTTTTTTAATTTCAAAATGAGTGATGAGAAGAAAATTGTTAAGTATCAACCAATGATCAATCTTCTCATAGATGGAGGCCTACAAGTTTGTGCCTTTCCAAGACCTCGTAAGTAGTTTCACTTCACTACAAAATTTGATGCATACATGTCAAATGTACTTTGACACCCATGATAGGTATGTCTAAGAGTGATTTTGAAATGCTATTAAAAATCACTCTGGAAAATGTCTTTAATATCGTTTCAATAATGTTTTTTTTTTAGTTCAACAATAATTGAGGGTGGGAGATACCATTGAGTTATGGTCATTTTGGCTTTTATCATGAGCTTCAATAATGTTAATAAAGTAATTTCTTTTTTTTTTTACTTTTTCTTTTAATTACCAATTCACCTCTTCCTTCATTTGGTTTAGACTTTAGAAAAGAAATGCTTATCTACTAATCGGACAGCAAAAATAGTGAAATCTCATTATCAACACCCAAATT

mRNA sequence

ATGATCGCTGTGAAATTTCTCTCAAACCCCATTTCAGTTGCAGCTCTATTGCTACTGATTTCCATTGTTGTAAGGCAGATTCTCAATGGAAAATCGAGGAGAAAGAAATATCATCCAATTGCAGGAACTGTATTTCACCAACTCCTCAATTTCCATCGATTGCATGATTATATGACTGATCTTGCTAGAAAGTATAAAACATATCGGCTTCTTGGTCCTTTCAGAAATGAGATTTACACTTCAGACCCACCAAATGTTGAGTATATACTCAGAACAAACTTCGAGAATTTTGGAAAGGGGTCACATAACCACAGAATTCTCAAGGATCTTCTTGGTGATGGAATTTTCACCGTTGATGGTGAGAAGTGGCGTCAGCAGAGGAAGATTTCAAGCTATGAGTTCTCTACAAAAGTTCTGAGGGATTTTAGCAGTGTCATATTCCAGAAATCTGCTGTTAAACTAGTTCATATCGTGTCCGAAGCTGCTATTTCAAATCGAGTAATTGATATTCAAGATCTCTTTATGAAAACAACTCTAGATTCAATTTTTCATGTATCATTTGGAATTGATCTGGATAGCTTGCATGGATCAAATGAAGAAATAAACAGGTTTTGCTCTGCTTTTGAAGCATCAAGTACAATGACACTCTGGCGATATGTTGATGTAATCTGGCCAATCAAGAAGATTCTGAATATTGGGTCTGAAGCTACCTTGAAAAAAAACATGAAAATTGTTGATGAGTACATATATAAGCTCATTCACAACGCACTCGAACAGCGACAGAAGTTAGGCGAGGACACTTTGATGACAAAAGATAACATAGTGTCGAGGTTTCTGCAAGCGGAAAAGACCAACCCGACGTATCTAAGAGATATTATTCTTAATTTTGTCATAGCTGGAAAGGACACAACAGCAGTTACACTTGCTTGGTTCATGTACGTGCTTTGCAAGCATCCTGACGTGCAGGAAAGAGTTGCTAGAGAGATCAAGGAAGTGACAAATGTTGAAGATGTTAACAATTTTGCAGAGTTTGCTGCAAGATTGACTGAAGAAGCTCTAGAGAAGATGCAGTTTCTCACTGCAACTATTAATGAAACTCTTAGACTCTATCCTGCTGTTCCAGTGGATGCAAAGATTTGCTTTTCTGACGATACATTGCCAGATGGATTCAGTGTGAGAAAAGGAGATATGGTGTCATACCAGCCATATGCAATGGGTAGAATGAAATTCATATGGGGTGAAGATGCCGAGGAATTCCGACCAGAGAGATGGTTTGATGAAGATGGAAATTTCCAGCCTCAGAGCCCTTTCAAATTTACAGCCTTTCAGGCAGGGCCACGCATTTGTCTGGGAAGAGACTTTGCTTACAGGCAGATGAAGATCCTCTCATCTCTATTATTGGTCTTTTTTAATTTCAAAATGAGTGATGAGAAGAAAATTGTTAAGTATCAACCAATGATCAATCTTCTCATAGATGGAGGCCTACAAGTTTGTGCCTTTCCAAGACCTCGTAAGTAG

Coding sequence (CDS)

ATGATCGCTGTGAAATTTCTCTCAAACCCCATTTCAGTTGCAGCTCTATTGCTACTGATTTCCATTGTTGTAAGGCAGATTCTCAATGGAAAATCGAGGAGAAAGAAATATCATCCAATTGCAGGAACTGTATTTCACCAACTCCTCAATTTCCATCGATTGCATGATTATATGACTGATCTTGCTAGAAAGTATAAAACATATCGGCTTCTTGGTCCTTTCAGAAATGAGATTTACACTTCAGACCCACCAAATGTTGAGTATATACTCAGAACAAACTTCGAGAATTTTGGAAAGGGGTCACATAACCACAGAATTCTCAAGGATCTTCTTGGTGATGGAATTTTCACCGTTGATGGTGAGAAGTGGCGTCAGCAGAGGAAGATTTCAAGCTATGAGTTCTCTACAAAAGTTCTGAGGGATTTTAGCAGTGTCATATTCCAGAAATCTGCTGTTAAACTAGTTCATATCGTGTCCGAAGCTGCTATTTCAAATCGAGTAATTGATATTCAAGATCTCTTTATGAAAACAACTCTAGATTCAATTTTTCATGTATCATTTGGAATTGATCTGGATAGCTTGCATGGATCAAATGAAGAAATAAACAGGTTTTGCTCTGCTTTTGAAGCATCAAGTACAATGACACTCTGGCGATATGTTGATGTAATCTGGCCAATCAAGAAGATTCTGAATATTGGGTCTGAAGCTACCTTGAAAAAAAACATGAAAATTGTTGATGAGTACATATATAAGCTCATTCACAACGCACTCGAACAGCGACAGAAGTTAGGCGAGGACACTTTGATGACAAAAGATAACATAGTGTCGAGGTTTCTGCAAGCGGAAAAGACCAACCCGACGTATCTAAGAGATATTATTCTTAATTTTGTCATAGCTGGAAAGGACACAACAGCAGTTACACTTGCTTGGTTCATGTACGTGCTTTGCAAGCATCCTGACGTGCAGGAAAGAGTTGCTAGAGAGATCAAGGAAGTGACAAATGTTGAAGATGTTAACAATTTTGCAGAGTTTGCTGCAAGATTGACTGAAGAAGCTCTAGAGAAGATGCAGTTTCTCACTGCAACTATTAATGAAACTCTTAGACTCTATCCTGCTGTTCCAGTGGATGCAAAGATTTGCTTTTCTGACGATACATTGCCAGATGGATTCAGTGTGAGAAAAGGAGATATGGTGTCATACCAGCCATATGCAATGGGTAGAATGAAATTCATATGGGGTGAAGATGCCGAGGAATTCCGACCAGAGAGATGGTTTGATGAAGATGGAAATTTCCAGCCTCAGAGCCCTTTCAAATTTACAGCCTTTCAGGCAGGGCCACGCATTTGTCTGGGAAGAGACTTTGCTTACAGGCAGATGAAGATCCTCTCATCTCTATTATTGGTCTTTTTTAATTTCAAAATGAGTGATGAGAAGAAAATTGTTAAGTATCAACCAATGATCAATCTTCTCATAGATGGAGGCCTACAAGTTTGTGCCTTTCCAAGACCTCGTAAGTAG

Protein sequence

MIAVKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTDLARKYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEKWRQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKKNMKIVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAGKDTTAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFRPERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKIVKYQPMINLLIDGGLQVCAFPRPRK

Homology

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BLAST of Spg031521 vs. NCBI nr
Match: KAA0049016.1 (cytochrome P450 704C1-like protein [Cucumis melo var. makuwa] >TYK17548.1 cytochrome P450 704C1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 883.6 bits (2282), Expect = 7.8e-253
Identity = 434/499 (86.97%), Postives = 472/499 (94.59%), Query Frame = 0


Query: 4   VKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTDLAR 63
           ++FLS PI VAA+LLLISIVVR IL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTDLAR
Sbjct: 1   MEFLSKPILVAAVLLLISIVVRLILTHKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLAR 60

Query: 64  KYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEKW 123
           K+KTY+LLGPFR+E+YTSDPPNVEY+LRTNFENFGKGSHNHRIL+DLLGDGIFTVDGEKW
Sbjct: 61  KHKTYKLLGPFRDEVYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKW 120

Query: 124 RQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIF 183
           R+QRKISSYEFSTKVLRD+SSVIF+K+AVKL +IVSEAAISNRVIDIQDLFMK+TLDSIF
Sbjct: 121 REQRKISSYEFSTKVLRDYSSVIFRKTAVKLANIVSEAAISNRVIDIQDLFMKSTLDSIF 180

Query: 184 HVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKKNMK 243
           +VSFGIDLDSLHGSNEEI++FCSAFEASST TLWRYVDV WPIKK LNIGSEA LK+NMK
Sbjct: 181 YVSFGIDLDSLHGSNEEISKFCSAFEASSTNTLWRYVDVSWPIKKALNIGSEAVLKENMK 240

Query: 244 IVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAGKDT 303
           IVDEYIYKLIH+ALEQRQKLG+D LM ++NIVSRFLQAE T+P YLRDIILNFVIAGKDT
Sbjct: 241 IVDEYIYKLIHSALEQRQKLGDDALMRRENIVSRFLQAETTDPKYLRDIILNFVIAGKDT 300

Query: 304 TAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLTATI 363
           TAVTLAWF+Y+LCKHP VQERVAREI+EVTNV DV +F+EFAARLTEEALEKMQFL ATI
Sbjct: 301 TAVTLAWFIYMLCKHPSVQERVAREIEEVTNVGDVTDFSEFAARLTEEALEKMQFLIATI 360

Query: 364 NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFRPER 423
           NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKF+WG+DAEEFRPER
Sbjct: 361 NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFVWGDDAEEFRPER 420

Query: 424 WFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKIVKY 483
           W D DG FQPQSPFKFTAFQAGPRICLG++FAYRQ+KI S+LLLV+F FKMSDEKKIV+Y
Sbjct: 421 WLDGDGKFQPQSPFKFTAFQAGPRICLGKEFAYRQIKIFSALLLVYFKFKMSDEKKIVEY 480

Query: 484 QPMINLLIDGGLQVCAFPR 503
           QPMINLLI GGLQVC  PR
Sbjct: 481 QPMINLLIKGGLQVCVIPR 499

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BLAST of Spg031521 vs. NCBI nr
Match: XP_022924418.1 (cytochrome P450 704C1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 882.9 bits (2280), Expect = 1.3e-252
Identity = 435/502 (86.65%), Postives = 473/502 (94.22%), Query Frame = 0


Query: 1   MIAVKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTD 60
           M+A KFLSNPISVAALLLLISIVVRQIL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTD
Sbjct: 8   MVAAKFLSNPISVAALLLLISIVVRQILARKLRKKKYHPIAGTVFHQLLNFHRLHDYMTD 67

Query: 61  LARKYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDG 120
           LARK+KTYRLLGPFRNEIYTSDPPNVEY+LRTNFENFGKGSHNHRIL+DLLGDGIFTVDG
Sbjct: 68  LARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDG 127

Query: 121 EKWRQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLD 180
           EKWRQQRKISSYEFST+VLR++SSVIF++SAVKL  IVS+A+ S + IDIQDLFMK+TLD
Sbjct: 128 EKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSKASTSGQAIDIQDLFMKSTLD 187

Query: 181 SIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKK 240
           SIFHVSFGIDLDSLHG NEE  +FC+AFEASSTMTLWRYVDV WP+KKILNIGSEATLK+
Sbjct: 188 SIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKE 247

Query: 241 NMKIVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAG 300
           N+KIVDEYIYKLIHNALEQRQKLG+ T M ++NIVSRFLQAE T+P YLRDIILNFVIAG
Sbjct: 248 NIKIVDEYIYKLIHNALEQRQKLGDKTSMRRENIVSRFLQAETTDPKYLRDIILNFVIAG 307

Query: 301 KDTTAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLT 360
           KDTTAVTLAWF+Y+LCKHP VQERVA+EI+EVTNVEDV +FAEFAARLTEE+LEKMQFLT
Sbjct: 308 KDTTAVTLAWFIYMLCKHPAVQERVAKEIEEVTNVEDVTDFAEFAARLTEESLEKMQFLT 367

Query: 361 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFR 420
           ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG+DAEEFR
Sbjct: 368 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFR 427

Query: 421 PERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKI 480
           PERW D DGN QPQSPFKFTAFQAGPRIC+G++FAYRQ+KI+ S+L+  F FKMSDE+KI
Sbjct: 428 PERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEEKI 487

Query: 481 VKYQPMINLLIDGGLQVCAFPR 503
           VKYQPMINLLI GGLQVCA PR
Sbjct: 488 VKYQPMINLLIKGGLQVCATPR 509

[Close]

BLAST of Spg031521 vs. NCBI nr
Match: KAG6582575.1 (Cytochrome P450 704C1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018959.1 Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 882.1 bits (2278), Expect = 2.3e-252
Identity = 434/502 (86.45%), Postives = 472/502 (94.02%), Query Frame = 0


Query: 1   MIAVKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTD 60
           M+A KFLSNPISVAALLLLISIVVRQIL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTD
Sbjct: 8   MVAAKFLSNPISVAALLLLISIVVRQILTRKLRKKKYHPIAGTVFHQLLNFHRLHDYMTD 67

Query: 61  LARKYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDG 120
           LARK+KTYRLLGPFRNEIYTSDPPNVEY+LRTNFENFGKGSHNHRIL+DLLGDGIFTVDG
Sbjct: 68  LARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDG 127

Query: 121 EKWRQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLD 180
           EKWRQQRKISSYEFST+VLR++SSVIF++SAVKL  IVS+A+ S + IDIQDLFMK+TLD
Sbjct: 128 EKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSKASTSGQAIDIQDLFMKSTLD 187

Query: 181 SIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKK 240
           SIFHV FGIDLDSLHG NEE  +FC+AFEASSTMTLWRYVDV WP+KKILNIGSEATLK+
Sbjct: 188 SIFHVLFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKE 247

Query: 241 NMKIVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAG 300
           N+KIVDEYIYKLIHNALEQRQKLG+ T M ++NIVSRFLQAE T+P YLRDIILNFVIAG
Sbjct: 248 NIKIVDEYIYKLIHNALEQRQKLGDKTSMRRENIVSRFLQAETTDPKYLRDIILNFVIAG 307

Query: 301 KDTTAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLT 360
           KDTTAVTLAWF+Y+LCKHP VQERVA+EI+EVTNVEDV +FAEFAARLTEE+LEKMQFLT
Sbjct: 308 KDTTAVTLAWFIYMLCKHPAVQERVAKEIEEVTNVEDVTDFAEFAARLTEESLEKMQFLT 367

Query: 361 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFR 420
           ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG+DAEEFR
Sbjct: 368 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFR 427

Query: 421 PERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKI 480
           PERW D DGN QPQSPFKFTAFQAGPRIC+G++FAYRQ+KI+ S+L+  F FKMSDE+KI
Sbjct: 428 PERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEEKI 487

Query: 481 VKYQPMINLLIDGGLQVCAFPR 503
           VKYQPMINLLI GGLQVCA PR
Sbjct: 488 VKYQPMINLLIKGGLQVCAIPR 509

[Close]

BLAST of Spg031521 vs. NCBI nr
Match: XP_008438128.1 (PREDICTED: cytochrome P450 704C1-like [Cucumis melo])

HSP 1 Score: 881.3 bits (2276), Expect = 3.9e-252
Identity = 433/499 (86.77%), Postives = 471/499 (94.39%), Query Frame = 0


Query: 4   VKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTDLAR 63
           ++FLS PI VAA+LLLISIVVR IL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTDLAR
Sbjct: 1   MEFLSKPILVAAVLLLISIVVRLILTHKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLAR 60

Query: 64  KYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEKW 123
           K+KTY+LLGPFR+E+YTSDPPNVEY+LRTNFENFGKGSHNHRIL+DLLGDGIFTVDGEKW
Sbjct: 61  KHKTYKLLGPFRDEVYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKW 120

Query: 124 RQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIF 183
           R+QRKISSYEFSTKVLRD+SSVIF+K+AVKL +IVSEAAISNRVIDIQDLFMK+TLDSIF
Sbjct: 121 REQRKISSYEFSTKVLRDYSSVIFRKTAVKLANIVSEAAISNRVIDIQDLFMKSTLDSIF 180

Query: 184 HVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKKNMK 243
           +VSFGIDLDSLHGSNEEI++FCSAFEASST TLWRYVDV WPIKK LNIGSEA LK+NMK
Sbjct: 181 YVSFGIDLDSLHGSNEEISKFCSAFEASSTNTLWRYVDVSWPIKKALNIGSEAVLKENMK 240

Query: 244 IVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAGKDT 303
           IVDEYIYKLIH+ALEQRQKLG+D LM ++NIVSRFLQAE T+P YLRDIILNFVIAGKDT
Sbjct: 241 IVDEYIYKLIHSALEQRQKLGDDALMRRENIVSRFLQAETTDPKYLRDIILNFVIAGKDT 300

Query: 304 TAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLTATI 363
           TAVTLAWF+Y+LCKHP VQERVAREI+E TNV DV +F+EFAARLTEEALEKMQFL ATI
Sbjct: 301 TAVTLAWFIYMLCKHPSVQERVAREIEEETNVGDVTDFSEFAARLTEEALEKMQFLIATI 360

Query: 364 NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFRPER 423
           NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKF+WG+DAEEFRPER
Sbjct: 361 NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFVWGDDAEEFRPER 420

Query: 424 WFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKIVKY 483
           W D DG FQPQSPFKFTAFQAGPRICLG++FAYRQ+KI S+LLLV+F FKMSDEKKIV+Y
Sbjct: 421 WLDGDGKFQPQSPFKFTAFQAGPRICLGKEFAYRQIKIFSALLLVYFKFKMSDEKKIVEY 480

Query: 484 QPMINLLIDGGLQVCAFPR 503
           QPMINLLI GGLQVC  PR
Sbjct: 481 QPMINLLIKGGLQVCVIPR 499

[Close]

BLAST of Spg031521 vs. NCBI nr
Match: XP_022980270.1 (cytochrome P450 704C1 [Cucurbita maxima])

HSP 1 Score: 880.9 bits (2275), Expect = 5.1e-252
Identity = 435/502 (86.65%), Postives = 470/502 (93.63%), Query Frame = 0


Query: 1   MIAVKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTD 60
           M+A KFLSNPISVAALLLLISIVVRQIL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTD
Sbjct: 8   MVAAKFLSNPISVAALLLLISIVVRQILTRKQRKKKYHPIAGTVFHQLLNFHRLHDYMTD 67

Query: 61  LARKYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDG 120
           LARK+KTYRLLG FRNEIYTSDPPNVEYILRTNFENFGKGSHNHRIL+DLLGDGIFTVDG
Sbjct: 68  LARKHKTYRLLGLFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILRDLLGDGIFTVDG 127

Query: 121 EKWRQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLD 180
           EKWRQQRKISSYEFST+VLR++SSVIF++SAVKL  IVSEA+ S + IDIQDLFMK+TLD
Sbjct: 128 EKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSEASTSGQAIDIQDLFMKSTLD 187

Query: 181 SIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKK 240
           SIFHVSFGIDLDSLHG NEE  +FC+AFEASSTMTLWRYVDV WP+KKILNIGSEATLK+
Sbjct: 188 SIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKE 247

Query: 241 NMKIVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAG 300
           N+KIVDEYIYKLIHN LEQRQKL + T M ++NIVSRFLQAE T+P YLRDIILNFVIAG
Sbjct: 248 NIKIVDEYIYKLIHNTLEQRQKLCDKTSMRRENIVSRFLQAETTDPKYLRDIILNFVIAG 307

Query: 301 KDTTAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLT 360
           KDTTAVTLAWF+Y+LCKHP VQERVA+EIKEVTNVEDV +FA+FAARLTEE+LEKMQFLT
Sbjct: 308 KDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDVTDFAQFAARLTEESLEKMQFLT 367

Query: 361 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFR 420
           ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG+DAEEFR
Sbjct: 368 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFR 427

Query: 421 PERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKI 480
           PERW D DGN QPQSPFKFTAFQAGPRIC+G++FAYRQ+KI+ S+L+  F FKMSDEKKI
Sbjct: 428 PERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEKKI 487

Query: 481 VKYQPMINLLIDGGLQVCAFPR 503
           VKYQPMINLLI GGLQVCA PR
Sbjct: 488 VKYQPMINLLIKGGLQVCAIPR 509

[Close]

BLAST of Spg031521 vs. ExPASy Swiss-Prot
Match: Q50EK3 (Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.8e-135
Identity = 246/516 (47.67%), Postives = 362/516 (70.16%), Query Frame = 0


Query: 4   VKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTDLAR 63
           V  L+  ++V+AL L  S+ +   L    ++  Y P+ GT+ +  +NF RLHDY TD A+
Sbjct: 3   VNILTMFVTVSALALACSLWIASYLRNWRKKGVYPPVVGTMLNHAINFERLHDYHTDQAQ 62

Query: 64  KYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEKW 123
           +YKT+R++ P  + ++T+DP NVE+IL+TNF N+ KG+ N+ I+KDLLGDGIF VDG+KW
Sbjct: 63  RYKTFRVVYPTCSYVFTTDPVNVEHILKTNFANYDKGTFNYDIMKDLLGDGIFNVDGDKW 122

Query: 124 RQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIF 183
           RQQRK++S EF++KVL+DFSS +F  +A KL +I+++AA  N  +++QDLFM+++LDSI 
Sbjct: 123 RQQRKLASSEFASKVLKDFSSGVFCNNAAKLANILAQAAKLNLSVEMQDLFMRSSLDSIC 182

Query: 184 HVSFGIDLDSLHGSNEEIN---RFCSAFEASSTMTLWRY-VDVIWPIKKILNIGSEATLK 243
            V FGID++SL  S  E      F  AF+ ++ M   R+ V   W +++  N+GSEA L+
Sbjct: 183 KVVFGIDINSLSSSKAESGPEASFAKAFDVANAMVFHRHMVGSFWKVQRFFNVGSEAILR 242

Query: 244 KNMKIVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAE------KTNPTYLRDII 303
            N+K+VD+++YK+IH    +++    +    + +I+SR++         K +  YLRD+I
Sbjct: 243 DNIKMVDDFLYKVIH--FRRQEMFSAEKENVRPDILSRYIIISDKETDGKVSDKYLRDVI 302

Query: 304 LNFVIAGKDTTAVTLAWFMYVLCKHPDVQERVAREIKEVTNVE------DVNNFAEFAAR 363
           LNF++A +DTTA+ L+WF+Y+LCKH  VQE++  EI   T+V       + N+ A FA  
Sbjct: 303 LNFMVAARDTTAIALSWFIYMLCKHQHVQEKLLEEIISSTSVHEDQYSTECNDIASFAQS 362

Query: 364 LTEEALEKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGR 423
           LT+EAL KM +L A+++ETLRLYPA+PVD K   ++DTLPDGF V+KGD V++ PYAMGR
Sbjct: 363 LTDEALGKMHYLHASLSETLRLYPALPVDGKYVVNEDTLPDGFKVKKGDSVNFLPYAMGR 422

Query: 424 MKFIWGEDAEEFRPERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLL 483
           M ++WG+DA+EF+PERW  +DG F P+SPFKF AFQAGPR CLG+DFAY QMKI++++L+
Sbjct: 423 MSYLWGDDAKEFKPERWI-QDGIFHPKSPFKFPAFQAGPRTCLGKDFAYLQMKIVAAVLV 482

Query: 484 VFFNFKMSDEKKIVKYQPMINL-LIDGGLQVCAFPR 503
            FF F+    K+ V+Y+ M+ L + + GL V   PR
Sbjct: 483 RFFKFEAVKTKE-VRYRTMLTLHMNEDGLNVQVTPR 514

[Close]

BLAST of Spg031521 vs. ExPASy Swiss-Prot
Match: Q9C788 (Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 2.1e-107
Identity = 212/493 (43.00%), Postives = 305/493 (61.87%), Query Frame = 0


Query: 36  KYHPIAGTVFHQLLNFHRLHDYMTDLARKYKTYRLLGPFRNEIYTSDPPNVEYILRTNFE 95
           K  P+ G    QL NF R+HD++ +     +T  +  PF    Y +DP NVEY+L+TNF 
Sbjct: 29  KTWPLVGAAIEQLTNFDRMHDWLVEYLYNSRTVVVPMPFTTYTYIADPINVEYVLKTNFS 88

Query: 96  NFGKGSHNHRILKDLLGDGIFTVDGEKWRQQRKISSYEFSTKVLRDFSSVIFQKSAVKLV 155
           N+ KG   H  ++ LLGDGIF  DGE WR+QRK +S+EF++K LRDFS+V+F++ ++KL 
Sbjct: 89  NYPKGETYHSYMEVLLGDGIFNSDGELWRKQRKTASFEFASKNLRDFSTVVFKEYSLKLF 148

Query: 156 HIVSEAAISNRVIDIQDLFMKTTLDSIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMT 215
            I+S+A+   + +D+Q+L M+ TLDSI  V FG+++ +L     E N F  AF+ ++ + 
Sbjct: 149 TILSQASFKEQQVDMQELLMRMTLDSICKVGFGVEIGTLAPELPE-NHFAKAFDTANIIV 208

Query: 216 LWRYVDVIWPIKKILNIGSEATLKKNMKIVDEYIY--------KLIHNALEQRQKLGEDT 275
             R++D +W +KK LNIGSEA L K++K+V+++ Y        +L+   +        + 
Sbjct: 209 TLRFIDPLWKMKKFLNIGSEALLGKSIKVVNDFTYSVIRRRKAELLEAQISPTNNNNNNN 268

Query: 276 LMTKDNIVSRFLQA-----EKTNPTYLRDIILNFVIAGKDTTAVTLAWFMYVLCKHPDVQ 335
              K +I+SRF++       K     LRDI+LNFVIAG+DTTA TL W +Y++  + +V 
Sbjct: 269 NKVKHDILSRFIEISDDPDSKETEKSLRDIVLNFVIAGRDTTATTLTWAIYMIMMNENVA 328

Query: 336 ERVAREIKEVT--------------NVEDVNNF----AEFAARLTEEALEKMQFLTATIN 395
           E++  E++E+               + ED N+F     EFA  L  ++L K+ +L A I 
Sbjct: 329 EKLYSELQELEKESAEATNTSLHQYDTEDFNSFNEKVTEFAGLLNYDSLGKLHYLHAVIT 388

Query: 396 ETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFRPERW 455
           ETLRLYPAVP D K    DD LP+G  V+ G MV+Y PY+MGRM++ WG DA  F+PERW
Sbjct: 389 ETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMVTYVPYSMGRMEYNWGSDAALFKPERW 448

Query: 456 FDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKIVKYQ 498
             +DG FQ  SPFKFTAFQAGPRICLG+D AY QMK+  ++L  F+ F +      VKY+
Sbjct: 449 L-KDGVFQNASPFKFTAFQAGPRICLGKDSAYLQMKMAMAILCRFYKFHLVPNHP-VKYR 508

[Close]

BLAST of Spg031521 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.1e-79
Identity = 181/519 (34.87%), Postives = 300/519 (57.80%), Query Frame = 0


Query: 11  ISVAALLLLISIVVRQILNGKSRRKKYH--------PIAGTVFHQLLNFHRLHDYMTDLA 70
           + V+  +++++IV   +L  KS  K           P+ G++   + N +R+H+++ D  
Sbjct: 1   MEVSTTMMIVAIVAAYLLWFKSITKSMKGPKGPTMWPVVGSLPGLIENGNRMHEWIADNL 60

Query: 71  R----KYKTYRLLGPF--RNEI--YTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDG 130
           R     Y+T     PF  R ++   T DP N+E+IL+  F+N+ KG     +  DLLG+G
Sbjct: 61  RACSGTYQTCICAIPFLARKQLVTVTCDPKNLEHILKVRFDNYPKGPTWQAVFHDLLGEG 120

Query: 131 IFTVDGEKWRQQRKISSYEFSTKVLRD-FSSVIFQKSAVKLVHIVSEAAISNRVIDIQDL 190
           IF  DG+ W  QRK ++ EF+T+ LR      + +    +   I+  A +  + +D+QDL
Sbjct: 121 IFNSDGDTWLFQRKTAALEFTTRTLRQAMGRWVNRAIKNRFCPILEMAQVQGKPVDLQDL 180

Query: 191 FMKTTLDSIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYV--DVIWPIKKILN 250
            ++ T D+I  ++FG D ++L     E N F ++F+ ++  TL R++  + +W +KK+L 
Sbjct: 181 LLRLTFDNICGLAFGKDPETLSPELPE-NNFATSFDRATEATLHRFIMPEFVWKLKKMLG 240

Query: 251 IGSEATLKKNMKIVDEYIYKLIHN---ALEQRQKLGEDTLMTKDNIVSRFL-QAEKTNPT 310
           +G E +L  ++K VD Y+  +I+     L   Q  G       D+++SRF+ + E  +  
Sbjct: 241 LGLEVSLNNSLKQVDNYMTDVINTRKLELLNHQNGGPQ----HDDLLSRFMKKKESYSDK 300

Query: 311 YLRDIILNFVIAGKDTTAVTLAWFMYVLCKHPDVQERVAREIKEV---TNVEDVNNFAEF 370
           +L+ + LNF++AG+DT++V L+WF +++  +P V+E++  EI  +   T   D + + E 
Sbjct: 301 FLQHVALNFILAGRDTSSVALSWFFWLVSSNPRVEEKILVEICTILAETRGNDTSKWLE- 360

Query: 371 AARLTEEALEKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYA 430
              L  E ++++ +L A ++ETLRLYP+VP D+K   SDD LPDG  V  G  ++Y  Y+
Sbjct: 361 -EPLVFEEVDQLMYLKAALSETLRLYPSVPEDSKHVISDDYLPDGTFVPAGSNITYSIYS 420

Query: 431 MGRMKFIWGEDAEEFRPERWFDEDGN-FQPQSPFKFTAFQAGPRICLGRDFAYRQMKILS 490
            GRMKFIWGED  EF+PERW  +DG+ FQ Q  F+F AF AGPRICLG+D AY QMK ++
Sbjct: 421 TGRMKFIWGEDCLEFKPERWMSQDGDKFQVQDTFRFVAFNAGPRICLGKDLAYLQMKSIA 480

Query: 491 SLLLVFFNFKMSDEKKIVKYQPMINLLIDGGLQVCAFPR 503
           + +L+     ++   K V+ +  + L +  GL +   PR
Sbjct: 481 AAVLLRHRLAVAPGHK-VEQKMSLTLFMKDGLVMNVTPR 511

[Close]

BLAST of Spg031521 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.4e-79
Identity = 180/508 (35.43%), Postives = 298/508 (58.66%), Query Frame = 0


Query: 13  VAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNF------HRLHDYMTD-LARKY 72
           V  L LLI+I V   ++   R+KKY  +    F  +L          ++++++D L  + 
Sbjct: 36  VQILELLIAIFVFVAIHA-LRQKKYQGLPVWPFLGMLPSLAFGLRGNIYEWLSDVLCLQN 95

Query: 73  KTYRLLGPF---RNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEK 132
            T++  GP+    N   T DP NVE++L+  F  F KGS+    L+DLLGDGIF  D E 
Sbjct: 96  GTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGSYFRDNLRDLLGDGIFNADDET 155

Query: 133 WRQQRKISSYEFSTKVLRDFSS-VIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDS 192
           W++QRK +S EF +   R  ++  +F+    +L+ ++  +  S+  ID+QD+ ++ T D+
Sbjct: 156 WQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLETSVKSSSPIDLQDVLLRLTFDN 215

Query: 193 IFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYV--DVIWPIKKILNIGSEATLK 252
           +  ++FG+D   L G ++ +  F  AFE ++   + R+V    +W   + L+IG+E  LK
Sbjct: 216 VCMIAFGVDPGCL-GPDQPVIPFAKAFEDATEAAVVRFVMPTCVWKFMRYLDIGTEKKLK 275

Query: 253 KNMKIVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFL-----QAEKTNPTYLRDIIL 312
           +++K VD++  ++I    ++    GE T   + ++++ F+     + E  +  +LRDI +
Sbjct: 276 ESIKGVDDFADEVIRTRKKELSLEGETT--KRSDLLTVFMGLRDEKGESFSDKFLRDICV 335

Query: 313 NFVIAGKDTTAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAE---FAARLTEE 372
           NF++AG+DT++V L+WF ++L K+P+V+E++  E+ ++    D +  AE   +      E
Sbjct: 336 NFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKILRQRDDHGNAEKSDYEPVFGPE 395

Query: 373 ALEKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFI 432
            ++KM +L A ++E LRLYP+VPVD K    DD  PDG  ++KGD V Y  YAMGRM+ I
Sbjct: 396 EIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGTMLKKGDKVIYAIYAMGRMEAI 455

Query: 433 WGEDAEEFRPERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFN 491
           WG+D  EFRPERW   DG F  +S +KFTAF  GPR+CLG+DFAY QMK  ++ ++  + 
Sbjct: 456 WGKDCLEFRPERWL-RDGRFMSESAYKFTAFNGGPRLCLGKDFAYYQMKSTAAAIVYRYK 515

[Close]

BLAST of Spg031521 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.0e-78
Identity = 175/477 (36.69%), Postives = 286/477 (59.96%), Query Frame = 0


Query: 12  SVAALLLLISIVVRQILNGKSRRKKYH-----PIAGTVFHQLLNFHRLHDYMTDLAR--- 71
           S AA++L  +++V  I+   S R+ Y      P+ G++   + N HR+HD++ D  R   
Sbjct: 4   STAAIIL--TLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMCG 63

Query: 72  -KYKTYRLLGPF-----RNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFT 131
             Y+T     PF      +   T DP N+E+IL+T F+N+ KG     +  DLLGDGIF 
Sbjct: 64  GTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIFN 123

Query: 132 VDGEKWRQQRKISSYEFSTKVLRD-FSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMK 191
            DG+ WR QRK ++ EF+T+ LR   +  + +    +LV I+  A      ID+QD+ ++
Sbjct: 124 SDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLLR 183

Query: 192 TTLDSIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYV--DVIWPIKKILNIGS 251
            T D+I  ++FG D  +L     E N F  AF+ ++  TL R++  + IW I+K L +G 
Sbjct: 184 LTFDNICGLTFGKDPRTLSPEFPE-NGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLGL 243

Query: 252 EATLKKNMKIVDEYIYKLIHNALEQRQKLGEDTLMTK-DNIVSRFL-QAEKTNPTYLRDI 311
           E  + +++  VD Y+ ++I+    + + LG+    ++ D+++SRF+ + E  +  YL+ +
Sbjct: 244 EDDMSRSISHVDNYLSEIINT--RKLELLGQQQDESRHDDLLSRFMKKKESYSDKYLKYV 303

Query: 312 ILNFVIAGKDTTAVTLAWFMYVLCKHPDVQERVAREIKEV-TNVEDVNNFAEFAARLTEE 371
            LNF++AG+DT++V ++WF +++  +P V+E++  EI  +     D N        LT +
Sbjct: 304 ALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDEPLTFD 363

Query: 372 ALEKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFI 431
            ++++ +L A ++ETLRLYP+VP D+K   ++D LPDG  V  G  V+Y  Y++GRMKFI
Sbjct: 364 EIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFI 423

Query: 432 WGEDAEEFRPERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLV 469
           WGED  EF+PERW +E  + +  + +KF AF AGPRICLG+D AY QMK +++ +L+
Sbjct: 424 WGEDCLEFKPERWLEESRD-EKCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILL 474

[Close]

BLAST of Spg031521 vs. ExPASy TrEMBL
Match: A0A5D3D249 (Cytochrome P450 704C1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004180 PE=3 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 3.8e-253
Identity = 434/499 (86.97%), Postives = 472/499 (94.59%), Query Frame = 0


Query: 4   VKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTDLAR 63
           ++FLS PI VAA+LLLISIVVR IL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTDLAR
Sbjct: 1   MEFLSKPILVAAVLLLISIVVRLILTHKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLAR 60

Query: 64  KYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEKW 123
           K+KTY+LLGPFR+E+YTSDPPNVEY+LRTNFENFGKGSHNHRIL+DLLGDGIFTVDGEKW
Sbjct: 61  KHKTYKLLGPFRDEVYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKW 120

Query: 124 RQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIF 183
           R+QRKISSYEFSTKVLRD+SSVIF+K+AVKL +IVSEAAISNRVIDIQDLFMK+TLDSIF
Sbjct: 121 REQRKISSYEFSTKVLRDYSSVIFRKTAVKLANIVSEAAISNRVIDIQDLFMKSTLDSIF 180

Query: 184 HVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKKNMK 243
           +VSFGIDLDSLHGSNEEI++FCSAFEASST TLWRYVDV WPIKK LNIGSEA LK+NMK
Sbjct: 181 YVSFGIDLDSLHGSNEEISKFCSAFEASSTNTLWRYVDVSWPIKKALNIGSEAVLKENMK 240

Query: 244 IVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAGKDT 303
           IVDEYIYKLIH+ALEQRQKLG+D LM ++NIVSRFLQAE T+P YLRDIILNFVIAGKDT
Sbjct: 241 IVDEYIYKLIHSALEQRQKLGDDALMRRENIVSRFLQAETTDPKYLRDIILNFVIAGKDT 300

Query: 304 TAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLTATI 363
           TAVTLAWF+Y+LCKHP VQERVAREI+EVTNV DV +F+EFAARLTEEALEKMQFL ATI
Sbjct: 301 TAVTLAWFIYMLCKHPSVQERVAREIEEVTNVGDVTDFSEFAARLTEEALEKMQFLIATI 360

Query: 364 NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFRPER 423
           NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKF+WG+DAEEFRPER
Sbjct: 361 NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFVWGDDAEEFRPER 420

Query: 424 WFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKIVKY 483
           W D DG FQPQSPFKFTAFQAGPRICLG++FAYRQ+KI S+LLLV+F FKMSDEKKIV+Y
Sbjct: 421 WLDGDGKFQPQSPFKFTAFQAGPRICLGKEFAYRQIKIFSALLLVYFKFKMSDEKKIVEY 480

Query: 484 QPMINLLIDGGLQVCAFPR 503
           QPMINLLI GGLQVC  PR
Sbjct: 481 QPMINLLIKGGLQVCVIPR 499

[Close]

BLAST of Spg031521 vs. ExPASy TrEMBL
Match: A0A6J1ECD4 (cytochrome P450 704C1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431924 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 6.5e-253
Identity = 435/502 (86.65%), Postives = 473/502 (94.22%), Query Frame = 0


Query: 1   MIAVKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTD 60
           M+A KFLSNPISVAALLLLISIVVRQIL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTD
Sbjct: 8   MVAAKFLSNPISVAALLLLISIVVRQILARKLRKKKYHPIAGTVFHQLLNFHRLHDYMTD 67

Query: 61  LARKYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDG 120
           LARK+KTYRLLGPFRNEIYTSDPPNVEY+LRTNFENFGKGSHNHRIL+DLLGDGIFTVDG
Sbjct: 68  LARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDG 127

Query: 121 EKWRQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLD 180
           EKWRQQRKISSYEFST+VLR++SSVIF++SAVKL  IVS+A+ S + IDIQDLFMK+TLD
Sbjct: 128 EKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSKASTSGQAIDIQDLFMKSTLD 187

Query: 181 SIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKK 240
           SIFHVSFGIDLDSLHG NEE  +FC+AFEASSTMTLWRYVDV WP+KKILNIGSEATLK+
Sbjct: 188 SIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKE 247

Query: 241 NMKIVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAG 300
           N+KIVDEYIYKLIHNALEQRQKLG+ T M ++NIVSRFLQAE T+P YLRDIILNFVIAG
Sbjct: 248 NIKIVDEYIYKLIHNALEQRQKLGDKTSMRRENIVSRFLQAETTDPKYLRDIILNFVIAG 307

Query: 301 KDTTAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLT 360
           KDTTAVTLAWF+Y+LCKHP VQERVA+EI+EVTNVEDV +FAEFAARLTEE+LEKMQFLT
Sbjct: 308 KDTTAVTLAWFIYMLCKHPAVQERVAKEIEEVTNVEDVTDFAEFAARLTEESLEKMQFLT 367

Query: 361 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFR 420
           ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG+DAEEFR
Sbjct: 368 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFR 427

Query: 421 PERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKI 480
           PERW D DGN QPQSPFKFTAFQAGPRIC+G++FAYRQ+KI+ S+L+  F FKMSDE+KI
Sbjct: 428 PERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEEKI 487

Query: 481 VKYQPMINLLIDGGLQVCAFPR 503
           VKYQPMINLLI GGLQVCA PR
Sbjct: 488 VKYQPMINLLIKGGLQVCATPR 509

[Close]

BLAST of Spg031521 vs. ExPASy TrEMBL
Match: A0A1S3AW87 (cytochrome P450 704C1-like OS=Cucumis melo OX=3656 GN=LOC103483328 PE=3 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 1.9e-252
Identity = 433/499 (86.77%), Postives = 471/499 (94.39%), Query Frame = 0


Query: 4   VKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTDLAR 63
           ++FLS PI VAA+LLLISIVVR IL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTDLAR
Sbjct: 1   MEFLSKPILVAAVLLLISIVVRLILTHKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLAR 60

Query: 64  KYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEKW 123
           K+KTY+LLGPFR+E+YTSDPPNVEY+LRTNFENFGKGSHNHRIL+DLLGDGIFTVDGEKW
Sbjct: 61  KHKTYKLLGPFRDEVYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKW 120

Query: 124 RQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIF 183
           R+QRKISSYEFSTKVLRD+SSVIF+K+AVKL +IVSEAAISNRVIDIQDLFMK+TLDSIF
Sbjct: 121 REQRKISSYEFSTKVLRDYSSVIFRKTAVKLANIVSEAAISNRVIDIQDLFMKSTLDSIF 180

Query: 184 HVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKKNMK 243
           +VSFGIDLDSLHGSNEEI++FCSAFEASST TLWRYVDV WPIKK LNIGSEA LK+NMK
Sbjct: 181 YVSFGIDLDSLHGSNEEISKFCSAFEASSTNTLWRYVDVSWPIKKALNIGSEAVLKENMK 240

Query: 244 IVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAGKDT 303
           IVDEYIYKLIH+ALEQRQKLG+D LM ++NIVSRFLQAE T+P YLRDIILNFVIAGKDT
Sbjct: 241 IVDEYIYKLIHSALEQRQKLGDDALMRRENIVSRFLQAETTDPKYLRDIILNFVIAGKDT 300

Query: 304 TAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLTATI 363
           TAVTLAWF+Y+LCKHP VQERVAREI+E TNV DV +F+EFAARLTEEALEKMQFL ATI
Sbjct: 301 TAVTLAWFIYMLCKHPSVQERVAREIEEETNVGDVTDFSEFAARLTEEALEKMQFLIATI 360

Query: 364 NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFRPER 423
           NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKF+WG+DAEEFRPER
Sbjct: 361 NETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFVWGDDAEEFRPER 420

Query: 424 WFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKIVKY 483
           W D DG FQPQSPFKFTAFQAGPRICLG++FAYRQ+KI S+LLLV+F FKMSDEKKIV+Y
Sbjct: 421 WLDGDGKFQPQSPFKFTAFQAGPRICLGKEFAYRQIKIFSALLLVYFKFKMSDEKKIVEY 480

Query: 484 QPMINLLIDGGLQVCAFPR 503
           QPMINLLI GGLQVC  PR
Sbjct: 481 QPMINLLIKGGLQVCVIPR 499

[Close]

BLAST of Spg031521 vs. ExPASy TrEMBL
Match: A0A6J1IVT5 (cytochrome P450 704C1 OS=Cucurbita maxima OX=3661 GN=LOC111479691 PE=3 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 2.5e-252
Identity = 435/502 (86.65%), Postives = 470/502 (93.63%), Query Frame = 0


Query: 1   MIAVKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTD 60
           M+A KFLSNPISVAALLLLISIVVRQIL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTD
Sbjct: 8   MVAAKFLSNPISVAALLLLISIVVRQILTRKQRKKKYHPIAGTVFHQLLNFHRLHDYMTD 67

Query: 61  LARKYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDG 120
           LARK+KTYRLLG FRNEIYTSDPPNVEYILRTNFENFGKGSHNHRIL+DLLGDGIFTVDG
Sbjct: 68  LARKHKTYRLLGLFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILRDLLGDGIFTVDG 127

Query: 121 EKWRQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLD 180
           EKWRQQRKISSYEFST+VLR++SSVIF++SAVKL  IVSEA+ S + IDIQDLFMK+TLD
Sbjct: 128 EKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSEASTSGQAIDIQDLFMKSTLD 187

Query: 181 SIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKK 240
           SIFHVSFGIDLDSLHG NEE  +FC+AFEASSTMTLWRYVDV WP+KKILNIGSEATLK+
Sbjct: 188 SIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKE 247

Query: 241 NMKIVDEYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFLQAEKTNPTYLRDIILNFVIAG 300
           N+KIVDEYIYKLIHN LEQRQKL + T M ++NIVSRFLQAE T+P YLRDIILNFVIAG
Sbjct: 248 NIKIVDEYIYKLIHNTLEQRQKLCDKTSMRRENIVSRFLQAETTDPKYLRDIILNFVIAG 307

Query: 301 KDTTAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKMQFLT 360
           KDTTAVTLAWF+Y+LCKHP VQERVA+EIKEVTNVEDV +FA+FAARLTEE+LEKMQFLT
Sbjct: 308 KDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDVTDFAQFAARLTEESLEKMQFLT 367

Query: 361 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFR 420
           ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG+DAEEFR
Sbjct: 368 ATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFR 427

Query: 421 PERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKI 480
           PERW D DGN QPQSPFKFTAFQAGPRIC+G++FAYRQ+KI+ S+L+  F FKMSDEKKI
Sbjct: 428 PERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEKKI 487

Query: 481 VKYQPMINLLIDGGLQVCAFPR 503
           VKYQPMINLLI GGLQVCA PR
Sbjct: 488 VKYQPMINLLIKGGLQVCAIPR 509

[Close]

BLAST of Spg031521 vs. ExPASy TrEMBL
Match: A0A6J1E8W4 (cytochrome P450 704C1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431924 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 1.6e-251
Identity = 435/506 (85.97%), Postives = 473/506 (93.48%), Query Frame = 0


Query: 1   MIAVKFLSNPISVAALLLLISIVVRQILNGKSRRKKYHPIAGTVFHQLLNFHRLHDYMTD 60
           M+A KFLSNPISVAALLLLISIVVRQIL  K R+KKYHPIAGTVFHQLLNFHRLHDYMTD
Sbjct: 8   MVAAKFLSNPISVAALLLLISIVVRQILARKLRKKKYHPIAGTVFHQLLNFHRLHDYMTD 67

Query: 61  LARKYKTYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDG 120
           LARK+KTYRLLGPFRNEIYTSDPPNVEY+LRTNFENFGKGSHNHRIL+DLLGDGIFTVDG
Sbjct: 68  LARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDG 127

Query: 121 EKWRQQRKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLD 180
           EKWRQQRKISSYEFST+VLR++SSVIF++SAVKL  IVS+A+ S + IDIQDLFMK+TLD
Sbjct: 128 EKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSKASTSGQAIDIQDLFMKSTLD 187

Query: 181 SIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKK 240
           SIFHVSFGIDLDSLHG NEE  +FC+AFEASSTMTLWRYVDV WP+KKILNIGSEATLK+
Sbjct: 188 SIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKE 247

Query: 241 NMKIVDEYIYKLIHNALEQRQKLGE----DTLMTKDNIVSRFLQAEKTNPTYLRDIILNF 300
           N+KIVDEYIYKLIHNALEQRQKLG+    D  M ++NIVSRFLQAE T+P YLRDIILNF
Sbjct: 248 NIKIVDEYIYKLIHNALEQRQKLGDKTSVDEFMRRENIVSRFLQAETTDPKYLRDIILNF 307

Query: 301 VIAGKDTTAVTLAWFMYVLCKHPDVQERVAREIKEVTNVEDVNNFAEFAARLTEEALEKM 360
           VIAGKDTTAVTLAWF+Y+LCKHP VQERVA+EI+EVTNVEDV +FAEFAARLTEE+LEKM
Sbjct: 308 VIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIEEVTNVEDVTDFAEFAARLTEESLEKM 367

Query: 361 QFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDA 420
           QFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG+DA
Sbjct: 368 QFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDA 427

Query: 421 EEFRPERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSD 480
           EEFRPERW D DGN QPQSPFKFTAFQAGPRIC+G++FAYRQ+KI+ S+L+  F FKMSD
Sbjct: 428 EEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSD 487

Query: 481 EKKIVKYQPMINLLIDGGLQVCAFPR 503
           E+KIVKYQPMINLLI GGLQVCA PR
Sbjct: 488 EEKIVKYQPMINLLIKGGLQVCATPR 513

[Close]

BLAST of Spg031521 vs. TAIR 10
Match: AT2G45510.1 (cytochrome P450, family 704, subfamily A, polypeptide 2 )

HSP 1 Score: 506.9 bits (1304), Expect = 1.9e-143
Identity = 260/497 (52.31%), Postives = 348/497 (70.02%), Query Frame = 0


Query: 16  LLLLISIVVRQILNGKSRR-KKYHPIAGTVFHQLLNFHRLHDYMTDLARKYKTYRLLGPF 75
           L   I +++R I  GKSR  K+Y P+  TVF  L +   L+DY T++AR+  TYR L P 
Sbjct: 19  LCFTIYLMIR-IFTGKSRNDKRYAPVHATVFDLLFHSDELYDYETEIAREKPTYRFLSPG 78

Query: 76  RNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEKWRQQRKISSYEF 135
           ++EI T+DP NVE+IL+T F+N+ KG  +   + DLLG GIF VDGEKWRQQRK+SS+EF
Sbjct: 79  QSEILTADPRNVEHILKTRFDNYSKGHSSRENMADLLGHGIFAVDGEKWRQQRKLSSFEF 138

Query: 136 STKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIFHVSFGIDLDSL 195
           ST+VLRDFS  +F+++A KLV  VSE A+S +  D QDL M+ TLDSIF V FG++L  L
Sbjct: 139 STRVLRDFSCSVFRRNASKLVGFVSEFALSGKAFDAQDLLMRCTLDSIFKVGFGVELKCL 198

Query: 196 HGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKKNMKIVDEYIYKLIH 255
            G ++E   F  AF+  +  T  R++D +W +K   NIGS++ LKK++  +D+++Y LI 
Sbjct: 199 DGFSKEGQEFMEAFDEGNVATSSRFIDPLWKLKWFFNIGSQSKLKKSIATIDKFVYSLI- 258

Query: 256 NALEQRQKLGEDTLMTKDNIVSRFL-----QAEKTNPTYLRDIILNFVIAGKDTTAVTLA 315
              ++++   E   + +++I+SRFL       E  N  YLRDIILNF+IAGKDTTA  L+
Sbjct: 259 -TTKRKELAKEQNTVVREDILSRFLVESEKDPENMNDKYLRDIILNFMIAGKDTTAALLS 318

Query: 316 WFMYVLCKHPDVQERVAREIKEVT----NVEDVNNFAEFAARLTEEALEKMQFLTATINE 375
           WF+Y+LCK+P VQE++ +EI++VT       DVN F E    + EEAL++M +L A ++E
Sbjct: 319 WFLYMLCKNPLVQEKIVQEIRDVTFSHEKTTDVNGFVE---SINEEALDEMHYLHAALSE 378

Query: 376 TLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFRPERWF 435
           TLRLYP VPVD +   +DD LPDG  V KGD + Y  YAMGRM +IWG+DAEEF+PERW 
Sbjct: 379 TLRLYPPVPVDMRCAENDDVLPDGHRVSKGDNIYYIAYAMGRMTYIWGQDAEEFKPERWL 438

Query: 436 DEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKIVKYQP 495
            +DG FQP+SPFKF +F AGPRICLG+DFAYRQMKI+S  LL FF FKM+DE   V Y+ 
Sbjct: 439 -KDGLFQPESPFKFISFHAGPRICLGKDFAYRQMKIVSMALLHFFRFKMADENSKVYYKR 498

Query: 496 MINLLIDGGLQVCAFPR 503
           M+ L +DGGL +CA PR
Sbjct: 499 MLTLHVDGGLHLCAIPR 508

[Close]

BLAST of Spg031521 vs. TAIR 10
Match: AT2G44890.1 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 488.8 bits (1257), Expect = 5.2e-138
Identity = 254/505 (50.30%), Postives = 344/505 (68.12%), Query Frame = 0


Query: 11  ISVAALLLLISIVVR---QILNGKSRR-KKYHPIAGTVFHQLLNFHRLHDYMTDLARKYK 70
           I V  + +L+S  +    +I  GKSR  K+Y P+  T+F    + H+L+DY T++AR   
Sbjct: 4   IVVTTIFILLSFALYLTIRIFTGKSRNDKRYTPVHATIFDLFFHSHKLYDYETEIARTKP 63

Query: 71  TYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEKWRQQ 130
           T+R L P ++EI+T+DP NVE+IL+T F N+ KG      L DLLG GIF VDGEKW+QQ
Sbjct: 64  TFRFLSPGQSEIFTADPRNVEHILKTRFHNYSKGPVGTVNLADLLGHGIFAVDGEKWKQQ 123

Query: 131 RKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIFHVS 190
           RK+ S+EFST+VLR+FS  +F+ SA KLV  ++E A+S +  D QD+ MK TLDSIF V 
Sbjct: 124 RKLVSFEFSTRVLRNFSYSVFRTSASKLVGFIAEFALSGKSFDFQDMLMKCTLDSIFKVG 183

Query: 191 FGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKKNMKIVD 250
           FG++L  L G ++E   F  AF+  +  T  R  D  W +K  LNIGSE+ LKK++ I+D
Sbjct: 184 FGVELGCLDGFSKEGEEFMKAFDEGNGATSSRVTDPFWKLKCFLNIGSESRLKKSIAIID 243

Query: 251 EYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFL-----QAEKTNPTYLRDIILNFVIAGK 310
           +++Y LI    ++  K  E     +++I+S+FL       E  N  YLRDIILN ++AGK
Sbjct: 244 KFVYSLITTKRKELSK--EQNTSVREDILSKFLLESEKDPENMNDKYLRDIILNVMVAGK 303

Query: 311 DTTAVTLAWFMYVLCKHPDVQERVAREIKEVTN----VEDVNNFAEFAARLTEEALEKMQ 370
           DTTA +L+WF+Y+LCK+P VQE++ +EI++VT+      DVN F E    +TEEAL +MQ
Sbjct: 304 DTTAASLSWFLYMLCKNPLVQEKIVQEIRDVTSSHEKTTDVNGFIE---SVTEEALAQMQ 363

Query: 371 FLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAE 430
           +L A ++ET+RLYP VP   +   +DD LPDG  V KGD + Y  YAMGRM +IWG+DAE
Sbjct: 364 YLHAALSETMRLYPPVPEHMRCAENDDVLPDGHRVSKGDNIYYISYAMGRMTYIWGQDAE 423

Query: 431 EFRPERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDE 490
           EF+PERW  +DG FQP+S FKF +F AGPRIC+G+DFAYRQMKI+S  LL FF FKM+DE
Sbjct: 424 EFKPERWL-KDGVFQPESQFKFISFHAGPRICIGKDFAYRQMKIVSMALLHFFRFKMADE 483

Query: 491 KKIVKYQPMINLLIDGGLQVCAFPR 503
              V Y+ M+ L +DGGL +CA PR
Sbjct: 484 NSKVSYKKMLTLHVDGGLHLCAIPR 502

[Close]

BLAST of Spg031521 vs. TAIR 10
Match: AT2G44890.2 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 458.0 bits (1177), Expect = 9.9e-129
Identity = 246/505 (48.71%), Postives = 335/505 (66.34%), Query Frame = 0


Query: 11  ISVAALLLLISIVVR---QILNGKSRR-KKYHPIAGTVFHQLLNFHRLHDYMTDLARKYK 70
           I V  + +L+S  +    +I  GKSR  K+Y P+  T+F    + H+L+DY T++AR   
Sbjct: 10  IVVTTIFILLSFALYLTIRIFTGKSRNDKRYTPVHATIFDLFFHSHKLYDYETEIARTKP 69

Query: 71  TYRLLGPFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILKDLLGDGIFTVDGEKWRQQ 130
           T+R L P ++EI+T+DP NVE+IL+T F N+ KG      L DLLG GIF VDGEKW+QQ
Sbjct: 70  TFRFLSPGQSEIFTADPRNVEHILKTRFHNYSKGPVGTVNLADLLGHGIFAVDGEKWKQQ 129

Query: 131 RKISSYEFSTKVLRDFSSVIFQKSAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIFHVS 190
           RK+ S+EFST+VLR+FS  +F+ SA KLV  ++E A+S +  D QD+ MK TLDSIF V 
Sbjct: 130 RKLVSFEFSTRVLRNFSYSVFRTSASKLVGFIAEFALSGKSFDFQDMLMKCTLDSIFKVG 189

Query: 191 FGIDLDSLHGSNEEINRFCSAFEASSTMTLWRYVDVIWPIKKILNIGSEATLKKNMKIVD 250
           FG++L  L G ++E   F  AF+  +  T  R  D  W +K  LNIGSE+ LKK++ I+D
Sbjct: 190 FGVELGCLDGFSKEGEEFMKAFDEGNGATSSRVTDPFWKLKCFLNIGSESRLKKSIAIID 249

Query: 251 EYIYKLIHNALEQRQKLGEDTLMTKDNIVSRFL-----QAEKTNPTYLRDIILNFVIAGK 310
           +++Y LI     +R++L ++      +I+S+FL       E  N  YLRDIILN ++AGK
Sbjct: 250 KFVYSLI---TTKRKELSKE---QNTDILSKFLLESEKDPENMNDKYLRDIILNVMVAGK 309

Query: 311 DTTAVTLAWFMYVLCKHPDVQERVAREIKEVTN----VEDVNNFAEFAARLTEEALEKMQ 370
           DTTA +L+WF+Y+LCK+P VQE++ +EI++VT+      DVN F E    +TEEAL +MQ
Sbjct: 310 DTTAASLSWFLYMLCKNPLVQEKIVQEIRDVTSSHEKTTDVNGFIE---SVTEEALAQMQ 369

Query: 371 FLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAE 430
           +L A ++ET+RLYP VP   +   +DD LPDG  V KGD + Y  YAMGRM +IWG+DAE
Sbjct: 370 YLHAALSETMRLYPPVPEHMRCAENDDVLPDGHRVSKGDNIYYISYAMGRMTYIWGQDAE 429

Query: 431 EFRPERWFDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDE 490
           EF+PERW  +DG              AGPRIC+G+DFAYRQMKI+S  LL FF FKM+DE
Sbjct: 430 EFKPERWL-KDG--------------AGPRICIGKDFAYRQMKIVSMALLHFFRFKMADE 489

Query: 491 KKIVKYQPMINLLIDGGLQVCAFPR 503
              V Y+ M+ L +DGGL +CA PR
Sbjct: 490 NSKVSYKKMLTLHVDGGLHLCAIPR 490

[Close]

BLAST of Spg031521 vs. TAIR 10
Match: AT1G69500.1 (cytochrome P450, family 704, subfamily B, polypeptide 1 )

HSP 1 Score: 391.0 bits (1003), Expect = 1.5e-108
Identity = 212/493 (43.00%), Postives = 305/493 (61.87%), Query Frame = 0


Query: 36  KYHPIAGTVFHQLLNFHRLHDYMTDLARKYKTYRLLGPFRNEIYTSDPPNVEYILRTNFE 95
           K  P+ G    QL NF R+HD++ +     +T  +  PF    Y +DP NVEY+L+TNF 
Sbjct: 29  KTWPLVGAAIEQLTNFDRMHDWLVEYLYNSRTVVVPMPFTTYTYIADPINVEYVLKTNFS 88

Query: 96  NFGKGSHNHRILKDLLGDGIFTVDGEKWRQQRKISSYEFSTKVLRDFSSVIFQKSAVKLV 155
           N+ KG   H  ++ LLGDGIF  DGE WR+QRK +S+EF++K LRDFS+V+F++ ++KL 
Sbjct: 89  NYPKGETYHSYMEVLLGDGIFNSDGELWRKQRKTASFEFASKNLRDFSTVVFKEYSLKLF 148

Query: 156 HIVSEAAISNRVIDIQDLFMKTTLDSIFHVSFGIDLDSLHGSNEEINRFCSAFEASSTMT 215
            I+S+A+   + +D+Q+L M+ TLDSI  V FG+++ +L     E N F  AF+ ++ + 
Sbjct: 149 TILSQASFKEQQVDMQELLMRMTLDSICKVGFGVEIGTLAPELPE-NHFAKAFDTANIIV 208

Query: 216 LWRYVDVIWPIKKILNIGSEATLKKNMKIVDEYIY--------KLIHNALEQRQKLGEDT 275
             R++D +W +KK LNIGSEA L K++K+V+++ Y        +L+   +        + 
Sbjct: 209 TLRFIDPLWKMKKFLNIGSEALLGKSIKVVNDFTYSVIRRRKAELLEAQISPTNNNNNNN 268

Query: 276 LMTKDNIVSRFLQA-----EKTNPTYLRDIILNFVIAGKDTTAVTLAWFMYVLCKHPDVQ 335
              K +I+SRF++       K     LRDI+LNFVIAG+DTTA TL W +Y++  + +V 
Sbjct: 269 NKVKHDILSRFIEISDDPDSKETEKSLRDIVLNFVIAGRDTTATTLTWAIYMIMMNENVA 328

Query: 336 ERVAREIKEVT--------------NVEDVNNF----AEFAARLTEEALEKMQFLTATIN 395
           E++  E++E+               + ED N+F     EFA  L  ++L K+ +L A I 
Sbjct: 329 EKLYSELQELEKESAEATNTSLHQYDTEDFNSFNEKVTEFAGLLNYDSLGKLHYLHAVIT 388

Query: 396 ETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFRPERW 455
           ETLRLYPAVP D K    DD LP+G  V+ G MV+Y PY+MGRM++ WG DA  F+PERW
Sbjct: 389 ETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMVTYVPYSMGRMEYNWGSDAALFKPERW 448

Query: 456 FDEDGNFQPQSPFKFTAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKIVKYQ 498
             +DG FQ  SPFKFTAFQAGPRICLG+D AY QMK+  ++L  F+ F +      VKY+
Sbjct: 449 L-KDGVFQNASPFKFTAFQAGPRICLGKDSAYLQMKMAMAILCRFYKFHLVPNHP-VKYR 508

[Close]

BLAST of Spg031521 vs. TAIR 10
Match: AT3G56630.1 (cytochrome P450, family 94, subfamily D, polypeptide 2 )

HSP 1 Score: 326.6 bits (836), Expect = 3.4e-89
Identity = 187/478 (39.12%), Postives = 288/478 (60.25%), Query Frame = 0


Query: 36  KYHPIAGTVFHQLLNFHRLHDYMTDLARKYKTYRLLGPFRNE-----IYTSDPPNVEYIL 95
           K +PI G++   + N HR  D+  +   +  T   +  FR       + T++P NVEY+L
Sbjct: 32  KSYPIVGSLPGLVNNRHRFLDWTVETLSRCPTQTAI--FRRPGKLQFVMTANPANVEYML 91

Query: 96  RTNFENFGKGSHNHRILKDLLGDGIFTVDGEKWRQQRKISSYEFSTKVLRDF-SSVIFQK 155
           +T FE+F KG     IL+D LG GIF  DGE W +QRK +SYEFSTK LRDF  S +  +
Sbjct: 92  KTKFESFPKGERFISILEDFLGRGIFNSDGEMWWKQRKTASYEFSTKSLRDFVMSNVTVE 151

Query: 156 SAVKLVHIVSEAAISNRVIDIQDLFMKTTLDSIFHVSFGIDLDSLHGSNEEINRFCSAFE 215
              +LV +++EAA + ++ID+QD+  +   D+I  ++F +D   L         F  AFE
Sbjct: 152 INTRLVPVLAEAATNGKLIDLQDILERFAFDNICKLAFNVDSACLGDDGAAGVNFMQAFE 211

Query: 216 ASSTMTLWRYVDVI---WPIKKILNIGSEATLKKNMKIVDEYIYKLIHNALEQRQKLGED 275
            ++T+   R+  VI   W IKK LNIGSE  L++++ IV ++  +++ N +EQ  K+ + 
Sbjct: 212 TAATIISQRFQSVISYSWKIKKKLNIGSERVLRESIMIVHKFADEIVRNRIEQ-GKVSDH 271

Query: 276 TLMTKDNIVSRFLQAEKTN-PTYLRDIILNFVIAGKDTTAVTLAWFMYVLCKHPDVQERV 335
               K++++SRF+  E+ N P  LRDI+++F++AG+DTT+  L+WF ++L  HP+V++++
Sbjct: 272 ----KEDLLSRFISKEEMNSPEILRDIVISFILAGRDTTSSALSWFFWLLSMHPEVKDKI 331

Query: 336 AREIKEV-----TNVEDVNNFAEFAARLTEEALEKMQFLTATINETLRLYPAVPVDAKIC 395
            +E+  +       + +V  F         E L+ M +L A I E+LRLYP VPVD   C
Sbjct: 332 LQELNSIRERTGKRIGEVYGF---------EDLKLMNYLHAAITESLRLYPPVPVDTMSC 391

Query: 396 FSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGEDAEEFRPERWFDE-DGNFQPQSPFKF 455
             D+ LPDG  + K   +SY  YAMGRM+ IWG+D + F PERW DE +G F+ ++P+KF
Sbjct: 392 AEDNVLPDGTFIGKDWGISYNAYAMGRMESIWGKDCDRFDPERWIDETNGGFRGENPYKF 451

Query: 456 TAFQAGPRICLGRDFAYRQMKILSSLLLVFFNFKMSDEKKIVKYQPMINLLIDGGLQV 498
            AF AGPR+CLG++ AY QMK + + +L  F  ++  +K+  +    + L I GGL V
Sbjct: 452 PAFHAGPRMCLGKEMAYIQMKSIVAAVLERFVVEVPGKKERPEILMSVTLRIRGGLNV 493

The following BLAST results are available for this feature:

BLAST of Spg031521 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0049016.1	7.8e-253	86.97	cytochrome P450 704C1-like protein [Cucumis melo var. makuwa] >TYK17548.1 cytoch...	[more]
XP_022924418.1	1.3e-252	86.65	cytochrome P450 704C1 isoform X2 [Cucurbita moschata]	[more]
KAG6582575.1	2.3e-252	86.45	Cytochrome P450 704C1, partial [Cucurbita argyrosperma subsp. sororia] >KAG70189...	[more]
XP_008438128.1	3.9e-252	86.77	PREDICTED: cytochrome P450 704C1-like [Cucumis melo]	[more]
XP_022980270.1	5.1e-252	86.65	cytochrome P450 704C1 [Cucurbita maxima]	[more]

BLAST of Spg031521 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q50EK3	1.8e-135	47.67	Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1	[more]
Q9C788	2.1e-107	43.00	Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1	[more]
B3RFJ6	1.1e-79	34.87	Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1	[more]
Q9FMY1	1.4e-79	35.43	Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1	[more]
Q9CAD6	2.0e-78	36.69	Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1	[more]

BLAST of Spg031521 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D249	3.8e-253	86.97	Cytochrome P450 704C1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1ECD4	6.5e-253	86.65	cytochrome P450 704C1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431924 P...	[more]
A0A1S3AW87	1.9e-252	86.77	cytochrome P450 704C1-like OS=Cucumis melo OX=3656 GN=LOC103483328 PE=3 SV=1	[more]
A0A6J1IVT5	2.5e-252	86.65	cytochrome P450 704C1 OS=Cucurbita maxima OX=3661 GN=LOC111479691 PE=3 SV=1	[more]
A0A6J1E8W4	1.6e-251	85.97	cytochrome P450 704C1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431924 P...	[more]

BLAST of Spg031521 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45510.1	1.9e-143	52.31	cytochrome P450, family 704, subfamily A, polypeptide 2	[more]
AT2G44890.1	5.2e-138	50.30	cytochrome P450, family 704, subfamily A, polypeptide 1	[more]
AT2G44890.2	9.9e-129	48.71	cytochrome P450, family 704, subfamily A, polypeptide 1	[more]
AT1G69500.1	1.5e-108	43.00	cytochrome P450, family 704, subfamily B, polypeptide 1	[more]
AT3G56630.1	3.4e-89	39.12	cytochrome P450, family 94, subfamily D, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 360..378 score: 33.28 coord: 439..449 score: 46.58 coord: 308..334 score: 32.26 coord: 449..472 score: 25.31 coord: 288..305 score: 29.78
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 361..372 score: 48.59 coord: 440..449 score: 48.47 coord: 449..460 score: 35.35 coord: 299..316 score: 39.34
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 38..489 e-value: 6.7E-77 score: 259.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 30..499 e-value: 8.3E-104 score: 349.7
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 36..502
None	No IPR available	PANTHER	PTHR24296:SF210	CYTOCHROME P450-RELATED	coord: 11..503
None	No IPR available	PANTHER	PTHR24296	CYTOCHROME P450	coord: 11..503

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031521.1	Spg031521.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen