Spg031497 (gene) Sponge gourd (cylindrica) v1

BLAST of Spg031497 vs. NCBI nr
Match: XP_022980030.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980031.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980033.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980034.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 600/724 (82.87%), Postives = 646/724 (89.23%), Query Frame = 0

Query: 5   MGSQIPKSLRFGQPTSDFHDKYGGYQN-SRLSPRSILECIQRRFSSASESFRRRVMNLGF 64
           MGSQIPKS RFG PTSDFHDKYG +QN SRLSP SILE I+R+FSSA +S RR + +L F
Sbjct: 1   MGSQIPKSARFGHPTSDFHDKYGSHQNRSRLSPGSILESIERKFSSAYKSVRRIITHLSF 60

Query: 65  KNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLT 124
           K+ GDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPV D E +CLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDGERQCLT 120

Query: 125 RDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSN 184
            D QLMIIA VLRS +DLFY+LHMIFEFRTGFLPP+LPVFGSGELI+DPAAIAKRY+ SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLLSN 180

Query: 185 FLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRT 244
           FLIDILS+LPLPQLLVL I+PAAKGP+PLKTK+VVKIAVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCG 304
            GILTETAWSGAAFNLLIYMQ SHVVGAIWYLLS+E+QAKCW++AC+ +K C  + LYCG
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDK-CKDNFLYCG 300

Query: 305 DHSKKIYPFIEKYCPPKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVG 364
           +  ++I P+I++YC PKE ED KTFNFGMY+EAL+FQLT TTSFRRKFFYSFWWALRNV 
Sbjct: 301 NQRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHW 424
           SSGQNLQVSN+MGEVFFAVFIAILGLVLFA LISNIQKYLQSATVK+EQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPWSVT 544
           S M+KQLLD++CEYLKPVLFTEKSFILQEGD ID+MLFIMKG LA I T GWKND +S  
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKNDTFSAV 540

Query: 545 LKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNS 604
           L AGDFCGEELVQWA DPTSTSLPIS+R VKSLTEVEAFALKANELKSVTSQFHFQRLNS
Sbjct: 541 LNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQRLNS 600

Query: 605 KQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRG-GGNGRFQDAL 664
           KQFQLSVR             FYSHQW VWAAYKIQEAW +YRERK+RG  G+GRFQ AL
Sbjct: 601 KQFQLSVR-------------FYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQAAL 660

Query: 665 AETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQDRPNFT 724
           A+T GAS  FGATLYASIFISHLLQ VQRDQH +T QLTRVMTLPPPPKPDDE DRPN T
Sbjct: 661 AKTAGASVGFGATLYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVT 710

Query: 725 ILNL 727
           ILNL
Sbjct: 721 ILNL 710

BLAST of Spg031497 vs. NCBI nr
Match: XP_022924449.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >XP_022924450.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >KAG6582669.1 Cyclic nucleotide-gated ion channel 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 599/723 (82.85%), Postives = 647/723 (89.49%), Query Frame = 0

Query: 5   MGSQIPKSLRFGQPTSDFHDKYGGYQN-SRLSPRSILECIQRRFSSASESFRRRVMNLGF 64
           MGSQIPKS RFG PTSDFHDKYG YQN SRLSP SILE I+R+F SA +SFRR + +L F
Sbjct: 1   MGSQIPKSTRFGHPTSDFHDKYGSYQNGSRLSPGSILESIERKFLSAYKSFRRIITHLSF 60

Query: 65  KNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLT 124
           K+ GDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPV + E +CLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQCLT 120

Query: 125 RDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSN 184
            D QLMIIA VLRS +DLFY+LHMIFEFRTGFLPP+LPVFGSGELI+DPAAIAKRY+ SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLFSN 180

Query: 185 FLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRT 244
           FLIDILS+LPLPQLLVL I+PAA+GPVPLKTK++VKIAVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCG 304
            GILTETAWSGAAFNLLIYMQ SHVVGAIWYLLS+E+QAKCW++AC+ +K C  + LYCG
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDK-CKDNFLYCG 300

Query: 305 DHSKKIYPFIEKYCPPKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVG 364
           +  ++I P+I++YC PKESED KTFNFGMY+EAL+FQLT TTSFRRKFFYSFWWALRNV 
Sbjct: 301 NQRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHW 424
           SSGQNLQVSN+MGEVFFAVFIAILGLVLFA LISNIQKYLQSATVK+EQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPWSVT 544
           S M+KQLLD++CEYLKPVLFTEKSFILQEGD ID++LFIMKG LA I T GWKND  S  
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKNDTLSGV 540

Query: 545 LKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNS 604
           L AGDFCGEELVQWAMDPTSTSLPIS+R VKSLTEVEAFALKANELKSVTSQFHFQ LNS
Sbjct: 541 LNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQHLNS 600

Query: 605 KQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRG-GGNGRFQDAL 664
           KQFQLSVR             FYSHQW VWAAYK+QEAW DYRERK+RG  G+GRFQDAL
Sbjct: 601 KQFQLSVR-------------FYSHQWNVWAAYKVQEAWQDYRERKRRGVRGDGRFQDAL 660

Query: 665 AETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQDRPNFT 724
           A+T GAS  FGATLYASIFISHLLQ VQRDQH +TTQLT+VMTLPPPPKPDDE D PNFT
Sbjct: 661 AKTAGASVGFGATLYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFT 709

Query: 725 ILN 726
           ILN
Sbjct: 721 ILN 709

BLAST of Spg031497 vs. NCBI nr
Match: XP_023526877.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526878.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526879.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 592/723 (81.88%), Postives = 642/723 (88.80%), Query Frame = 0

Query: 5   MGSQIPKSLRFGQPTSDFHDKYGGYQN-SRLSPRSILECIQRRFSSASESFRRRVMNLGF 64
           MGSQIPKS RFG PTSDFHDKYG YQN SRLSP SILE I+R+FSSA +S RR + +  F
Sbjct: 1   MGSQIPKSTRFGHPTSDFHDKYGSYQNGSRLSPGSILESIERKFSSAYKSSRRIITHFSF 60

Query: 65  KNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLT 124
           K+ GDQSVKKPKF KKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPV + E +CLT
Sbjct: 61  KHPGDQSVKKPKFRKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQCLT 120

Query: 125 RDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSN 184
            D QLMIIA VLRS +DLFY+LHMIFEFRTGFLPP+LPVFGSGELI+DPAAIAKRY+ SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLFSN 180

Query: 185 FLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRT 244
           FLIDILS+LPLPQLLVL I+PAAKGP+PLKTK+VVKIAVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCG 304
            GILTETAWSGAAFNLLIYMQ SHVVGAIWYLLS+E+QAKCW++AC+  K C  + LYCG
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKGK-CKDNFLYCG 300

Query: 305 DHSKKIYPFIEKYCPPKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVG 364
           +  ++I+P+I++YC PKESED+KTF FGM++EAL+FQLT TTSFRRKFFYSFWWALRNV 
Sbjct: 301 NQRRQIHPYIDQYCSPKESEDEKTFKFGMFVEALKFQLTSTTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHW 424
           SSGQNLQVSN+MG+VFFAVFIAILGLVLFA LISNIQKYLQSATVK+EQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGDVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPWSVT 544
           S M+KQLLD++CEYLKPVLFTEKSFILQEGD ID+MLFIMKG LA   T GWKN+ +S  
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATSITYGWKNNTFSGV 540

Query: 545 LKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNS 604
           L AGDFCGEELVQWA DPTSTSLPIS+R VKSLTEVEAFALKANELKSVTSQFHFQRLNS
Sbjct: 541 LNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQRLNS 600

Query: 605 KQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRG-GGNGRFQDAL 664
           KQFQLSVR             FYSHQW VWAAYKIQEAW +YRERK+RG  G+GRFQDAL
Sbjct: 601 KQFQLSVR-------------FYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQDAL 660

Query: 665 AETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQDRPNFT 724
           A+  GAS  FGATLYASIFISHLLQ VQRDQ  +T QLTRVMTLPPPPKPDDE D PNFT
Sbjct: 661 AKMAGASVGFGATLYASIFISHLLQVVQRDQPQETAQLTRVMTLPPPPKPDDEPDSPNFT 709

Query: 725 ILN 726
           ILN
Sbjct: 721 ILN 709

BLAST of Spg031497 vs. NCBI nr
Match: XP_038891280.1 (cyclic nucleotide-gated ion channel 1-like [Benincasa hispida])

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 594/730 (81.37%), Postives = 643/730 (88.08%), Query Frame = 0

Query: 1   MSKFMGSQIPKSLRFGQPTSDFHDKYGGYQ-NSRLSPRSILECIQRRFSSASESFRRRVM 60
           M KFMGSQIPK +RFG PT+DFHD YG YQ  SRLSP SILE I+RRFSS S+SFRR + 
Sbjct: 1   MPKFMGSQIPKFVRFGHPTADFHDNYGCYQVRSRLSPGSILESIERRFSSVSDSFRRILT 60

Query: 61  NLGFKNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEH 120
            LGFK+SGDQSVKKPKFWKKILDP KPFLQQWNKIFVLSSVIAVAVDPLFFY+PVFD +H
Sbjct: 61  RLGFKHSGDQSVKKPKFWKKILDPHKPFLQQWNKIFVLSSVIAVAVDPLFFYVPVFDGKH 120

Query: 121 RCLTRDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRY 180
           +CLT D QLMI+A VLRSFIDLFYLLHMIFEFRTGFLPP+LPVFGSGELIKDPA IAK+Y
Sbjct: 121 QCLTMDRQLMIVACVLRSFIDLFYLLHMIFEFRTGFLPPSLPVFGSGELIKDPAKIAKKY 180

Query: 181 ISSNFLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYRE 240
           + SNFLID LSILPLPQLLVL I+PAAKGP+PLK K+  K+AV+LQYIPRL RIYPLYRE
Sbjct: 181 LFSNFLIDFLSILPLPQLLVLAIIPAAKGPIPLKMKDATKMAVILQYIPRLLRIYPLYRE 240

Query: 241 VTRTSGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACK-DNKDCIGD 300
           VTRTSGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIE+QAKCW+ ACK D+ +C G+
Sbjct: 241 VTRTSGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIERQAKCWIHACKEDDTNCTGN 300

Query: 301 VLYCGDHSKKIYPFIEKYCPPKESEDDKT-FNFGMYLEALQFQLTDTTSFRRKFFYSFWW 360
            LYCG+    +YP I K C PKES+D K  F FGMY+EAL+F LTDT SFRRKF YSFWW
Sbjct: 301 FLYCGNPKSHMYPSINKTCSPKESKDGKNGFEFGMYVEALKFNLTDTMSFRRKFCYSFWW 360

Query: 361 ALRNVGSSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINR 420
           ALRNVGSSGQNL+VS++MGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVK+EQMRI+R
Sbjct: 361 ALRNVGSSGQNLEVSHYMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKVEQMRISR 420

Query: 421 RDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALL 480
           RDAEHWMAHRMLP++LR RIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLA L
Sbjct: 421 RDAEHWMAHRMLPDELRHRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLAHL 480

Query: 481 KKVPLFSSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKN 540
           KKVPLFSSM+KQLLD++CEYLKPVLFTEKSFILQEGD ID+MLFIMKG LA I    WKN
Sbjct: 481 KKVPLFSSMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIIGCDWKN 540

Query: 541 DPWSVTLKAGDFCGEELVQWAMDPTS-TSLPISTRKVKSLTEVEAFALKANELKSVTSQF 600
           D +S TL AGDFCGEELV WAMDPTS TSLPIS R VK+LTEVEAF+LKANELKSVT+QF
Sbjct: 541 DLYSATLNAGDFCGEELVHWAMDPTSTTSLPISKRIVKTLTEVEAFSLKANELKSVTNQF 600

Query: 601 HFQRLNSKQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRGGGNG 660
           HFQRL+SKQFQLSVR             FYSHQWKVWAAYKIQEAWHD+RERK  G GNG
Sbjct: 601 HFQRLHSKQFQLSVR-------------FYSHQWKVWAAYKIQEAWHDFRERKLTGVGNG 660

Query: 661 RFQDALAETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQ 720
           RFQDALA+TVG S SF ATLYASIFISHLLQ VQRDQ+ QT   TRVMTLP PPKPDDEQ
Sbjct: 661 RFQDALAKTVGPSVSFSATLYASIFISHLLQTVQRDQYQQTPHFTRVMTLPAPPKPDDEQ 717

Query: 721 DRPNFTILNL 727
           D+PNFTIL+L
Sbjct: 721 DKPNFTILDL 717

BLAST of Spg031497 vs. NCBI nr
Match: XP_022147438.1 (cyclic nucleotide-gated ion channel 1-like [Momordica charantia])

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 587/728 (80.63%), Postives = 643/728 (88.32%), Query Frame = 0

Query: 1   MSKFMGSQIPKSLRFGQPTSDFHDKYGGYQN-SRLSPRSILECIQRRFSSASESFRRRVM 60
           MSKFMGS  PKSLRFG PTSD H +YGGYQN SRLSP SILE IQR+ SSASE FRR V 
Sbjct: 1   MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA 60

Query: 61  NLGFKNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEH 120
           NLGFK+  DQS KKP+FWKKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPV D++H
Sbjct: 61  NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH 120

Query: 121 RCLTRDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRY 180
           +CLT D  LMIIA VLRSFID+FYLLH+IFEFRTGFLPP+LPVFGSGEL++DPAAIAKRY
Sbjct: 121 QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 180

Query: 181 ISSNFLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYRE 240
           +SSNF+IDILSI+PLPQLL LVI+PAAKGP+PLKTKN +K+AVLLQY+PRLFRIYPLYRE
Sbjct: 181 LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE 240

Query: 241 VTRTSGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDV 300
           VTRTSGILTETAWSGAAFNLLIYM ASHVVGAIWYL S+EQ+++CW +AC+ N +C    
Sbjct: 241 VTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSVEQESRCWHEACRKN-NCNSKY 300

Query: 301 LYCGDHSKKIYPFIEKYCPPKESEDDKTFNFGMYLEALQFQLTD-TTSFRRKFFYSFWWA 360
           LYCGDHS+  Y FI++YCP KESEDDKTFNFG+YLEAL+F+LTD TT+F  KFFY FWWA
Sbjct: 301 LYCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWA 360

Query: 361 LRNVGSSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRR 420
           LRNVGSSGQNLQ+SN++GE+FFAVFIAILGLVLFA LISNIQKYLQS TVK+EQMRINRR
Sbjct: 361 LRNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRR 420

Query: 421 DAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLK 480
           DAEHWM HRMLPEDLRQRIRRYDQYKWQLNRGV EEELISNLPKDLRRDIKRHLCLALLK
Sbjct: 421 DAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLK 480

Query: 481 KVPLFSSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKND 540
           KVPLFSSM+KQLLD++CEYLKPVLFTEKS ILQEGD +D+MLFIMKGNLA I T GWKND
Sbjct: 481 KVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKND 540

Query: 541 PWSVTLKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHF 600
             S  LKAGDFCGEELV WAMDP STSLPISTR VK+LTEVEAFALKA EL+SV S F F
Sbjct: 541 SHSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLFRF 600

Query: 601 QRLNSKQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRGGGNGRF 660
           QR NS+QFQLSVR             FYSHQWKVWAAYKIQEAWHDY ERK+RGGG+ RF
Sbjct: 601 QRFNSQQFQLSVR-------------FYSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRF 660

Query: 661 QDALAETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQDR 720
           Q ALA+TVGASASFGATLYASIFISHLLQ VQRDQH QT+QL+RVMTLPPPPKP +    
Sbjct: 661 QGALAKTVGASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIER--- 710

Query: 721 PNFTILNL 727
            +FTIL+L
Sbjct: 721 -DFTILDL 710

BLAST of Spg031497 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 7.0e-197
Identity = 368/693 (53.10%), Postives = 492/693 (71.00%), Query Frame = 0

Query: 38  SILECIQRRFSSAS---ESFRRRVMNLGFKNSGDQSVKKPKFWKKILDPQKPFLQQWNKI 97
           SI +   R F S+S   + F+R   +  FK +  + +       KILDPQ PFLQ+WNKI
Sbjct: 42  SISDKFYRSFESSSARIKLFKRSYKSYSFKEAVSKGIGST---HKILDPQGPFLQRWNKI 101

Query: 98  FVLSSVIAVAVDPLFFYIPVFDKEHRCLTRDGQLMIIASVLRSFIDLFYLLHMIFEFRTG 157
           FVL+ +IAV++DPLFFY+P+ D   +CL  D ++ I ASVLRSF D+FY+LH+IF+FRTG
Sbjct: 102 FVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTG 161

Query: 158 FLPPTLPVFGSGELIKDPAAIAKRYISSNFLIDILSILPLPQLLVLVIVPAAKGPVPLKT 217
           F+ P+  VFG G L++D   IAKRY+SS+F+IDIL++LPLPQ+++L+I+P  +G   L T
Sbjct: 162 FIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLNT 221

Query: 218 KNVVKIAVLLQYIPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYMQASHVVGAIWY 277
           KN++K  V  QYIPR  RIYPLY+EVTRTSGILTETAW+GAAFNL +YM ASHV GA WY
Sbjct: 222 KNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFWY 281

Query: 278 LLSIEQQAKCWLDAC-KDNKDCIGDVLYCGDHSKKIYPFIEKYCPPKESEDDKTFNFGMY 337
           L SIE++  CW  AC ++N  CI  +LYC   +     F+ + C P ++ +   F+FG++
Sbjct: 282 LFSIERETVCWKQACERNNPPCISKLLYCDPETAGGNAFLNESC-PIQTPNTTLFDFGIF 341

Query: 338 LEALQFQLTDTTSFRRKFFYSFWWALRNVGSSGQNLQVSNHMGEVFFAVFIAILGLVLFA 397
           L+ALQ  + ++  F +KFFY FWW L+N+ S GQNL+ S ++ E+ FAVFI+I GLVLF+
Sbjct: 342 LDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLVLFS 401

Query: 398 FLISNIQKYLQSATVKIEQMRINRRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKE 457
           FLI N+Q YLQS T ++E+MR+ RRDAE WM+HR+LPE+LR+RIRRY+QYKWQ  RGV E
Sbjct: 402 FLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRGVDE 461

Query: 458 EELISNLPKDLRRDIKRHLCLALLKKVPLFSSMNKQLLDSVCEYLKPVLFTEKSFILQEG 517
           E L+SNLPKDLRRDIKRHLCLALL +VP+F  M++QLLD++C+ L+PVL+TE+S+I++EG
Sbjct: 462 ENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMDEQLLDALCDRLQPVLYTEESYIVREG 521

Query: 518 DQIDVMLFIMKGNLAAITTNGWKNDPW-SVTLKAGDFCGEELVQWAMDP-TSTSLPISTR 577
           D +D MLFIM+G L  ITTNG +     S  L AGDFCGEEL+ WA+DP +S++LPISTR
Sbjct: 522 DPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLGAGDFCGEELLTWALDPHSSSNLPISTR 581

Query: 578 KVKSLTEVEAFALKANELKSVTSQFHFQRLNSKQFQLSVRHVLTELMNLIYTLFYSHQWK 637
            V++L EVEAFALKA++LK V SQ  F+RL+SKQ + + R             +YS QWK
Sbjct: 582 TVRALMEVEAFALKADDLKFVASQ--FRRLHSKQLRHTFR-------------YYSQQWK 641

Query: 638 VWAAYKIQEAWHDYRERKKRGG---GNGRFQDALA-ETVGASASFGATLYASIFISHLLQ 697
            WAA  IQ AW  Y ++K          R QDALA E  G+S S GAT+YAS F +++L+
Sbjct: 642 TWAACFIQAAWRRYIKKKLEESLKEEENRLQDALAKEACGSSPSLGATIYASRFAANILR 701

Query: 698 AVQRDQHHQTTQLTR---VMTLPPPPKPDDEQD 718
            ++R    +  ++      M L  P +PD   D
Sbjct: 702 TIRRSGSVRKPRMPERMPPMLLQKPAEPDFNSD 715

BLAST of Spg031497 vs. ExPASy Swiss-Prot
Match: Q9LNJ0 (Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 GN=CNGC10 PE=2 SV=2)

HSP 1 Score: 653.7 bits (1685), Expect = 2.5e-186
Identity = 355/723 (49.10%), Postives = 481/723 (66.53%), Query Frame = 0

Query: 13  LRFGQPTSDFHDKYGGYQNSRLSPRSILECIQRRFSSASESFRRRVMNLGFKNSGDQSVK 72
           +RF         +YG  + +R S       + R F +    F++    L F +  D   K
Sbjct: 9   VRFKDEGKPLSSEYGYGRKARPS-------LDRVFKNVKWGFKK---PLSFPSHKDPDHK 68

Query: 73  KPK--FWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLTRDGQLMI 132
           +      K I++PQ  FLQ WNKIF+ + V+A+A+DPLFFYIP+ D    CLT D +L I
Sbjct: 69  ETSSVTRKNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEI 128

Query: 133 IASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSNFLIDILS 192
            AS+LR+ ID FY++H++F+FRT ++ P+  VFG GEL+ D  AIA +Y+SS F+ID+LS
Sbjct: 129 AASLLRTLIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLS 188

Query: 193 ILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRTSGILTET 252
           ILPLPQ++VL ++P+   PV L TK+ +K +++ QY+PR+ R+YPLY EVTRTSGI+TET
Sbjct: 189 ILPLPQIVVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTET 248

Query: 253 AWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCGDHSKKIY 312
           AW+GAA+NL +YM ASHV GA+WYL+S+E++ +CW +AC+  K C    LYC +      
Sbjct: 249 AWAGAAWNLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSN 308

Query: 313 PFIEKYCP---PKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVGSSGQ 372
            F+   CP   P +  +   FNFG++ +AL+  + ++  F +KFFY FWW LRN+ + GQ
Sbjct: 309 NFLTTSCPFLDPGDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQ 368

Query: 373 NLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHWMAHR 432
           NLQ S  +GE+ FA+ I I GLVLFA LI N+QKYL+S TV+ E+MR+ +RDAE WM+HR
Sbjct: 369 NLQTSKFVGEIIFAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHR 428

Query: 433 MLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMN 492
           MLPEDLR+RIRRY+QY+WQ  RGV+EE L+ NLPKDLRRDIKRHLCL LLKKVPLF  M+
Sbjct: 429 MLPEDLRKRIRRYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMD 488

Query: 493 KQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPW-SVTLKA 552
           +QLLD+VC+ L+PVL+TE S++++EGD +  MLF+M+G L + TTNG ++  + +V LKA
Sbjct: 489 EQLLDAVCDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKA 548

Query: 553 GDFCGEELVQWAMDPTSTS-LPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNSKQ 612
            DFCGE+L+ WA+DP S+S  PISTR V++LTEVEAFAL A +LKSV SQ  F+RL+SKQ
Sbjct: 549 SDFCGEDLLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQ--FRRLHSKQ 608

Query: 613 FQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRER---KKRGGGNGRFQDAL 672
            Q + R             FYS QW+ W+   IQ AW  Y  R   K       R ++AL
Sbjct: 609 LQHTFR-------------FYSVQWRTWSVSFIQAAWRRYCRRKLAKSLRDEEDRLREAL 668

Query: 673 AE--------TVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDD 718
           A         TV +S S G  LYAS F S+ L  ++    H  + L    TLP  P+   
Sbjct: 669 ASQDKEHNAATVSSSLSLGGALYASRFASNALHNLR----HNISNLPPRYTLPLLPQKPT 702

BLAST of Spg031497 vs. ExPASy Swiss-Prot
Match: Q9LD40 (Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 GN=CNGC13 PE=3 SV=2)

HSP 1 Score: 644.0 bits (1660), Expect = 2.0e-183
Identity = 357/715 (49.93%), Postives = 478/715 (66.85%), Query Frame = 0

Query: 25  KYG-GYQNSRLSPRSILECIQRRFSSASESFRRRVMNLGFKNSGDQSVKKPKFWKKILDP 84
           +YG G   +R S  ++L+ ++R          ++ ++ G  N    S       K I++P
Sbjct: 20  EYGYGRNKARPSLNTVLKNVRRGL--------KKPLSFGSHNKKRDSNSSTTTQKNIINP 79

Query: 85  QKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLTRDGQLMIIASVLRSFIDLFY 144
           Q  FLQ WNKIF+ +SVIA+A+DPLFFYIP+ D E  CL     L I ASVLR+FID FY
Sbjct: 80  QGSFLQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFY 139

Query: 145 LLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSNFLIDILSILPLPQLLVLVIV 204
           ++H++F+FRT ++ P+  VFG GEL+ DP AIA +Y+SS F+ID+LSILPLPQL+VL ++
Sbjct: 140 IIHIVFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVI 199

Query: 205 PAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYM 264
           P    PV L TK+ +   +  QYIPR+ RIYPLY EVTRTSGI+TETAW+GAA+NL +YM
Sbjct: 200 PNVNKPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYM 259

Query: 265 QASHVVGAIWYLLSIEQQAKCWLDAC-KDNKDCIGDVLYCGDHSKKIYPFIEKYCP---P 324
            ASHV GA+WYL+S+E++ +CW +AC K  + C    LYC  +S     F+   CP   P
Sbjct: 260 LASHVFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTSCPFINP 319

Query: 325 KESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVGSSGQNLQVSNHMGEVF 384
            +  +   FNFG++ +AL+  + ++  F +KFFY FWW LRN+ + GQNL  S  +GE+ 
Sbjct: 320 DDITNSTVFNFGIFTDALKSGIVESDDFWKKFFYCFWWGLRNLSALGQNLNTSKFVGEII 379

Query: 385 FAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHWMAHRMLPEDLRQRIRR 444
           FAV I I GLVLFA LI N+QKYL+S TV+ E+MR+ +RDAE WM+HRMLP+DLR+RIRR
Sbjct: 380 FAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDDLRKRIRR 439

Query: 445 YDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMNKQLLDSVCEYLK 504
           Y+QYKWQ  RGV+EE L+ NLPKDLRRDIKRH CL LLKKVPLF  M++QLLD+VC+ LK
Sbjct: 440 YEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPLFEIMDEQLLDAVCDKLK 499

Query: 505 PVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPW-SVTLKAGDFCGEELVQWA 564
           PVL+TE S+ ++EGD ++ MLF+M+G L + TTNG +   + +V LK  DFCGE+L+ WA
Sbjct: 500 PVLYTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCGEDLLTWA 559

Query: 565 MDPTSTS-LPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNSKQFQLSVRHVLTEL 624
           +DP S+S  PISTR V++LTEVEAFAL A++LK V SQ  F+RL+SKQ Q + R      
Sbjct: 560 LDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVASQ--FRRLHSKQLQHTFR------ 619

Query: 625 MNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRGG---GNGRFQDALAE---TVGASAS 684
                  FYS QW+ W A  IQ AW  +  RK          RF++A+ +      +S+S
Sbjct: 620 -------FYSVQWRTWGASFIQAAWRRHCRRKLARSLTEEEDRFRNAITKRERNAASSSS 679

Query: 685 FGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLP-PPPKPDDEQDRPNFTILN 726
             ATLYAS F S+ L+ +            R   LP  PPKP +    P+F++ N
Sbjct: 680 LVATLYASRFASNALRNL------------RTNNLPLLPPKPSE----PDFSLRN 695

BLAST of Spg031497 vs. ExPASy Swiss-Prot
Match: Q9SKD7 (Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 GN=CNGC3 PE=2 SV=2)

HSP 1 Score: 626.7 bits (1615), Expect = 3.3e-178
Identity = 340/720 (47.22%), Postives = 473/720 (65.69%), Query Frame = 0

Query: 5   MGSQIPKSLRFGQPTSDFHDKYGGYQNSRLSPRSILECIQRRFSSASESFRRRVMNLGFK 64
           M  Q  K +RF     +F  K      +R S  S+++ ++R F   SE  R     L   
Sbjct: 2   MNPQRNKFVRFNGNDDEFSTK-----TTRPSVSSVMKTVRRSFEKGSEKIRTFKRPLSVH 61

Query: 65  NSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLTR 124
           ++ ++   K K   ++++P   +LQ WNKIF+L SV+A+A DPLFFYIP    E  CL  
Sbjct: 62  SNKNKENNKKKKILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPERFCLNL 121

Query: 125 DGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSNF 184
           D +L  IA V R+FID FY++HM+F+F TGF+ P+   FG GEL +    IA RY+ S F
Sbjct: 122 DKKLQTIACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALRYLGSYF 181

Query: 185 LIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRTS 244
           LID+LSILP+PQ++VL IVP  + P  L  K ++K  +  QY+PR+ RIYPL++EVTRTS
Sbjct: 182 LIDLLSILPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKEVTRTS 241

Query: 245 GILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCGD 304
           G++TETAW+GAA NL +YM ASHV G+ WYL+SIE++ +CW +AC   ++C    LYC  
Sbjct: 242 GLVTETAWAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAYLYCSP 301

Query: 305 HSKKIYPFIEKYCP---PKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRN 364
             +    F+   CP   P+E  +   FNFG++ +ALQ  + ++  F +KFFY FWW LRN
Sbjct: 302 TGEDNRLFLNGSCPLIDPEEITNSTVFNFGIFADALQSGVVESRDFPKKFFYCFWWGLRN 361

Query: 365 VGSSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAE 424
           + + GQNL+ S   GE+ FA+ I I GLVLFA LI N+QKYLQS TV++E+MR+ RRDAE
Sbjct: 362 LSALGQNLKTSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMRVKRRDAE 421

Query: 425 HWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVP 484
            WM+HRMLP+DLR+RIR+Y+QYKWQ  +GV+EE L+S+LPKDLR+DIKRHLCL LLKKVP
Sbjct: 422 QWMSHRMLPDDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLCLKLLKKVP 481

Query: 485 LFSSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPW- 544
            F +M+ +LLD++C  LK VL+TEKS+I++EG+ ++ MLFIM+GNL + TT G +   + 
Sbjct: 482 WFQAMDDRLLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYGGRTGFFN 541

Query: 545 SVTLKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQR 604
           SV L AGDFCG +L+ WA+DP S+  PIS+R V++LTEVE F L A++LK V +Q  ++R
Sbjct: 542 SVDLVAGDFCG-DLLTWALDPLSSQFPISSRTVQALTEVEGFLLSADDLKFVATQ--YRR 601

Query: 605 LNSKQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRGG---GNGR 664
           L+SKQ    +RH+           FYS QW+ WAA  IQ AW  +  RK         G+
Sbjct: 602 LHSKQ----LRHMFR---------FYSVQWQTWAACFIQAAWKRHCRRKLSKALREEEGK 661

Query: 665 FQDALA--ETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDE 716
             + L   ++ G   + GA +YAS F SH L+ ++ +   + ++   ++TL P    D E
Sbjct: 662 LHNTLQNDDSGGNKLNLGAAIYASRFASHALRNLRANAAARNSRFPHMLTLLPQKPADPE 700

BLAST of Spg031497 vs. ExPASy Swiss-Prot
Match: Q8RWS9 (Probable cyclic nucleotide-gated ion channel 5 OS=Arabidopsis thaliana OX=3702 GN=CNGC5 PE=2 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 9.5e-170
Identity = 313/650 (48.15%), Postives = 435/650 (66.92%), Query Frame = 0

Query: 78  KKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLTRDGQLMIIASVLRS 137
           KKI DPQ  FL   NK+FV S +++V VDP FFY+PV + E +CL  D +L I AS LR+
Sbjct: 86  KKIFDPQDKFLLYCNKLFVASCILSVFVDPFFFYLPVINAESKCLGIDRKLAITASTLRT 145

Query: 138 FIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSNFLIDILSILPLPQL 197
           FID+FYL HM  + RT ++ P+  VFG GEL+ DPA IAKRY+   F+ID LS+LPLPQ+
Sbjct: 146 FIDVFYLAHMALQLRTAYIAPSSRVFGRGELVIDPAQIAKRYLQRWFIIDFLSVLPLPQI 205

Query: 198 LVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRTSGILTETAWSGAAF 257
           +V   + ++ G   L TK  +   VL+QYIPR  R+ PL  E+ RT+G+  ETAW+GAA+
Sbjct: 206 VVWRFLQSSNGSDVLATKQALLFIVLVQYIPRFLRVLPLTSELKRTAGVFAETAWAGAAY 265

Query: 258 NLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCGDHSKKIY------- 317
            LL+YM ASH+VGA WYLL++E+   CW +AC D  +C  D LYCG+ +   Y       
Sbjct: 266 YLLLYMLASHIVGAFWYLLALERNDACWQEACIDAGNCSTDFLYCGNQNMDGYAVWNRAK 325

Query: 318 -PFIEKYCPPKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVGSSGQNL 377
              ++  C     +++  F+FG+Y +AL   +  + +F  K+ Y  WW L+N+ + GQ L
Sbjct: 326 ESVLKSKCRADLDDNNPPFDFGIYTQALSSGIVSSQNFIVKYCYCLWWGLQNLSTLGQGL 385

Query: 378 QVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHWMAHRML 437
           + S +  E+ F++ +AI GL+LFA LI N+Q YLQS T+++E+MR+ RRD+E WM HRML
Sbjct: 386 ETSTYPMEIIFSISLAISGLILFALLIGNMQTYLQSLTIRLEEMRVKRRDSEQWMHHRML 445

Query: 438 PEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMNKQ 497
           P+DLR+R+RRYDQYKW   RGV EE L+ NLPKDLRRDIKRHLCLAL+++VPLF SM+ +
Sbjct: 446 PQDLRERVRRYDQYKWLETRGVDEEYLVQNLPKDLRRDIKRHLCLALVRRVPLFKSMDDK 505

Query: 498 LLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPWSVT-LKAGD 557
           LLD++C  LKP LFTE +++++EGD +D MLFI++G L ++TT+G ++  ++ + LK G+
Sbjct: 506 LLDAICMRLKPCLFTESTYLVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRSLLKEGE 565

Query: 558 FCGEELVQWAMDPTS-TSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNSKQFQ 617
           FCGEEL+ WA+DP S  +LP STR VK+LTEVEAFAL + ELK V SQ  F+RL+S+Q Q
Sbjct: 566 FCGEELLTWALDPKSGVNLPSSTRTVKALTEVEAFALTSEELKFVASQ--FRRLHSRQVQ 625

Query: 618 LSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRGGGNGRFQDALAETVG 677
            + R             FYSHQW+ WAA  IQ AW  Y +RKK            + T G
Sbjct: 626 HTFR-------------FYSHQWRTWAACFIQAAWRRYCKRKKMEEAEAEAAAVSSSTAG 685

Query: 678 ASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQD 718
            S S GA   A+ F ++ L+ + R+++   T++  ++ L  PP+PD   D
Sbjct: 686 PSYSIGAAFLATKFAANALRTIHRNRN---TKIRDLVKLQKPPEPDFTAD 717

BLAST of Spg031497 vs. ExPASy TrEMBL
Match: A0A6J1IV06 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479553 PE=4 SV=1)

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 600/724 (82.87%), Postives = 646/724 (89.23%), Query Frame = 0

Query: 5   MGSQIPKSLRFGQPTSDFHDKYGGYQN-SRLSPRSILECIQRRFSSASESFRRRVMNLGF 64
           MGSQIPKS RFG PTSDFHDKYG +QN SRLSP SILE I+R+FSSA +S RR + +L F
Sbjct: 1   MGSQIPKSARFGHPTSDFHDKYGSHQNRSRLSPGSILESIERKFSSAYKSVRRIITHLSF 60

Query: 65  KNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLT 124
           K+ GDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPV D E +CLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDGERQCLT 120

Query: 125 RDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSN 184
            D QLMIIA VLRS +DLFY+LHMIFEFRTGFLPP+LPVFGSGELI+DPAAIAKRY+ SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLLSN 180

Query: 185 FLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRT 244
           FLIDILS+LPLPQLLVL I+PAAKGP+PLKTK+VVKIAVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCG 304
            GILTETAWSGAAFNLLIYMQ SHVVGAIWYLLS+E+QAKCW++AC+ +K C  + LYCG
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDK-CKDNFLYCG 300

Query: 305 DHSKKIYPFIEKYCPPKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVG 364
           +  ++I P+I++YC PKE ED KTFNFGMY+EAL+FQLT TTSFRRKFFYSFWWALRNV 
Sbjct: 301 NQRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHW 424
           SSGQNLQVSN+MGEVFFAVFIAILGLVLFA LISNIQKYLQSATVK+EQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPWSVT 544
           S M+KQLLD++CEYLKPVLFTEKSFILQEGD ID+MLFIMKG LA I T GWKND +S  
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKNDTFSAV 540

Query: 545 LKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNS 604
           L AGDFCGEELVQWA DPTSTSLPIS+R VKSLTEVEAFALKANELKSVTSQFHFQRLNS
Sbjct: 541 LNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQRLNS 600

Query: 605 KQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRG-GGNGRFQDAL 664
           KQFQLSVR             FYSHQW VWAAYKIQEAW +YRERK+RG  G+GRFQ AL
Sbjct: 601 KQFQLSVR-------------FYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQAAL 660

Query: 665 AETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQDRPNFT 724
           A+T GAS  FGATLYASIFISHLLQ VQRDQH +T QLTRVMTLPPPPKPDDE DRPN T
Sbjct: 661 AKTAGASVGFGATLYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVT 710

Query: 725 ILNL 727
           ILNL
Sbjct: 721 ILNL 710

BLAST of Spg031497 vs. ExPASy TrEMBL
Match: A0A6J1EF20 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431950 PE=4 SV=1)

HSP 1 Score: 1181.0 bits (3054), Expect = 0.0e+00
Identity = 599/723 (82.85%), Postives = 647/723 (89.49%), Query Frame = 0

Query: 5   MGSQIPKSLRFGQPTSDFHDKYGGYQN-SRLSPRSILECIQRRFSSASESFRRRVMNLGF 64
           MGSQIPKS RFG PTSDFHDKYG YQN SRLSP SILE I+R+F SA +SFRR + +L F
Sbjct: 1   MGSQIPKSTRFGHPTSDFHDKYGSYQNGSRLSPGSILESIERKFLSAYKSFRRIITHLSF 60

Query: 65  KNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLT 124
           K+ GDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPV + E +CLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQCLT 120

Query: 125 RDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSN 184
            D QLMIIA VLRS +DLFY+LHMIFEFRTGFLPP+LPVFGSGELI+DPAAIAKRY+ SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLFSN 180

Query: 185 FLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRT 244
           FLIDILS+LPLPQLLVL I+PAA+GPVPLKTK++VKIAVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCG 304
            GILTETAWSGAAFNLLIYMQ SHVVGAIWYLLS+E+QAKCW++AC+ +K C  + LYCG
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDK-CKDNFLYCG 300

Query: 305 DHSKKIYPFIEKYCPPKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVG 364
           +  ++I P+I++YC PKESED KTFNFGMY+EAL+FQLT TTSFRRKFFYSFWWALRNV 
Sbjct: 301 NQRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHW 424
           SSGQNLQVSN+MGEVFFAVFIAILGLVLFA LISNIQKYLQSATVK+EQMRINRRDAEHW
Sbjct: 361 SSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPWSVT 544
           S M+KQLLD++CEYLKPVLFTEKSFILQEGD ID++LFIMKG LA I T GWKND  S  
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKNDTLSGV 540

Query: 545 LKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNS 604
           L AGDFCGEELVQWAMDPTSTSLPIS+R VKSLTEVEAFALKANELKSVTSQFHFQ LNS
Sbjct: 541 LNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQHLNS 600

Query: 605 KQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRG-GGNGRFQDAL 664
           KQFQLSVR             FYSHQW VWAAYK+QEAW DYRERK+RG  G+GRFQDAL
Sbjct: 601 KQFQLSVR-------------FYSHQWNVWAAYKVQEAWQDYRERKRRGVRGDGRFQDAL 660

Query: 665 AETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQDRPNFT 724
           A+T GAS  FGATLYASIFISHLLQ VQRDQH +TTQLT+VMTLPPPPKPDDE D PNFT
Sbjct: 661 AKTAGASVGFGATLYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFT 709

Query: 725 ILN 726
           ILN
Sbjct: 721 ILN 709

BLAST of Spg031497 vs. ExPASy TrEMBL
Match: A0A6J1D1B4 (cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC111016363 PE=4 SV=1)

HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 587/728 (80.63%), Postives = 643/728 (88.32%), Query Frame = 0

Query: 1   MSKFMGSQIPKSLRFGQPTSDFHDKYGGYQN-SRLSPRSILECIQRRFSSASESFRRRVM 60
           MSKFMGS  PKSLRFG PTSD H +YGGYQN SRLSP SILE IQR+ SSASE FRR V 
Sbjct: 1   MSKFMGSLKPKSLRFGYPTSDIHVRYGGYQNRSRLSPASILESIQRKISSASEGFRRMVA 60

Query: 61  NLGFKNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEH 120
           NLGFK+  DQS KKP+FWKKILDPQKPFLQQWNKIFVLS V+AVAVDPLFFYIPV D++H
Sbjct: 61  NLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIPVMDRQH 120

Query: 121 RCLTRDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRY 180
           +CLT D  LMIIA VLRSFID+FYLLH+IFEFRTGFLPP+LPVFGSGEL++DPAAIAKRY
Sbjct: 121 QCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPAAIAKRY 180

Query: 181 ISSNFLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYRE 240
           +SSNF+IDILSI+PLPQLL LVI+PAAKGP+PLKTKN +K+AVLLQY+PRLFRIYPLYRE
Sbjct: 181 LSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRIYPLYRE 240

Query: 241 VTRTSGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDV 300
           VTRTSGILTETAWSGAAFNLLIYM ASHVVGAIWYL S+EQ+++CW +AC+ N +C    
Sbjct: 241 VTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSVEQESRCWHEACRKN-NCNSKY 300

Query: 301 LYCGDHSKKIYPFIEKYCPPKESEDDKTFNFGMYLEALQFQLTD-TTSFRRKFFYSFWWA 360
           LYCGDHS+  Y FI++YCP KESEDDKTFNFG+YLEAL+F+LTD TT+F  KFFY FWWA
Sbjct: 301 LYCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFYCFWWA 360

Query: 361 LRNVGSSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRR 420
           LRNVGSSGQNLQ+SN++GE+FFAVFIAILGLVLFA LISNIQKYLQS TVK+EQMRINRR
Sbjct: 361 LRNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQMRINRR 420

Query: 421 DAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLK 480
           DAEHWM HRMLPEDLRQRIRRYDQYKWQLNRGV EEELISNLPKDLRRDIKRHLCLALLK
Sbjct: 421 DAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLCLALLK 480

Query: 481 KVPLFSSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKND 540
           KVPLFSSM+KQLLD++CEYLKPVLFTEKS ILQEGD +D+MLFIMKGNLA I T GWKND
Sbjct: 481 KVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITYGWKND 540

Query: 541 PWSVTLKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHF 600
             S  LKAGDFCGEELV WAMDP STSLPISTR VK+LTEVEAFALKA EL+SV S F F
Sbjct: 541 SHSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVVSLFRF 600

Query: 601 QRLNSKQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRGGGNGRF 660
           QR NS+QFQLSVR             FYSHQWKVWAAYKIQEAWHDY ERK+RGGG+ RF
Sbjct: 601 QRFNSQQFQLSVR-------------FYSHQWKVWAAYKIQEAWHDYCERKRRGGGDSRF 660

Query: 661 QDALAETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQDR 720
           Q ALA+TVGASASFGATLYASIFISHLLQ VQRDQH QT+QL+RVMTLPPPPKP +    
Sbjct: 661 QGALAKTVGASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIER--- 710

Query: 721 PNFTILNL 727
            +FTIL+L
Sbjct: 721 -DFTILDL 710

BLAST of Spg031497 vs. ExPASy TrEMBL
Match: A0A6J1IV11 (cyclic nucleotide-gated ion channel 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479553 PE=4 SV=1)

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 572/724 (79.01%), Postives = 617/724 (85.22%), Query Frame = 0

Query: 5   MGSQIPKSLRFGQPTSDFHDKYGGYQN-SRLSPRSILECIQRRFSSASESFRRRVMNLGF 64
           MGSQIPKS RFG PTSDFHDKYG +QN SRLSP SILE I+R+FSSA +S RR + +L F
Sbjct: 1   MGSQIPKSARFGHPTSDFHDKYGSHQNRSRLSPGSILESIERKFSSAYKSVRRIITHLSF 60

Query: 65  KNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLT 124
           K+ GDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPV D E +CLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDGERQCLT 120

Query: 125 RDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSN 184
            D QLMIIA VLRS +DLFY+LHMIFEFRTGFLPP+LPVFGSGELI+DPAAIAKRY+ SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLLSN 180

Query: 185 FLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRT 244
           FLIDILS+LPLPQLLVL I+PAAKGP+PLKTK+VVKIAVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCG 304
            GILTETAWSGAAFNLLIYMQ SHVVGAIWYLLS+E+QAKCW++AC+ +K C  + LYCG
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDK-CKDNFLYCG 300

Query: 305 DHSKKIYPFIEKYCPPKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVG 364
           +  ++I P+I++YC PKE ED KTFNFGMY+EAL+FQLT TTSFRRKFFYSFWWALRNV 
Sbjct: 301 NQRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHW 424
           SSGQNL                              QKYLQSATVK+EQMRINRRDAEHW
Sbjct: 361 SSGQNL------------------------------QKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPWSVT 544
           S M+KQLLD++CEYLKPVLFTEKSFILQEGD ID+MLFIMKG LA I T GWKND +S  
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKNDTFSAV 540

Query: 545 LKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNS 604
           L AGDFCGEELVQWA DPTSTSLPIS+R VKSLTEVEAFALKANELKSVTSQFHFQRLNS
Sbjct: 541 LNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQRLNS 600

Query: 605 KQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRG-GGNGRFQDAL 664
           KQFQLSVR             FYSHQW VWAAYKIQEAW +YRERK+RG  G+GRFQ AL
Sbjct: 601 KQFQLSVR-------------FYSHQWNVWAAYKIQEAWQEYRERKRRGVRGDGRFQAAL 660

Query: 665 AETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQDRPNFT 724
           A+T GAS  FGATLYASIFISHLLQ VQRDQH +T QLTRVMTLPPPPKPDDE DRPN T
Sbjct: 661 AKTAGASVGFGATLYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVT 680

Query: 725 ILNL 727
           ILNL
Sbjct: 721 ILNL 680

BLAST of Spg031497 vs. ExPASy TrEMBL
Match: A0A6J1E9H2 (cyclic nucleotide-gated ion channel 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431950 PE=4 SV=1)

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 571/723 (78.98%), Postives = 618/723 (85.48%), Query Frame = 0

Query: 5   MGSQIPKSLRFGQPTSDFHDKYGGYQN-SRLSPRSILECIQRRFSSASESFRRRVMNLGF 64
           MGSQIPKS RFG PTSDFHDKYG YQN SRLSP SILE I+R+F SA +SFRR + +L F
Sbjct: 1   MGSQIPKSTRFGHPTSDFHDKYGSYQNGSRLSPGSILESIERKFLSAYKSFRRIITHLSF 60

Query: 65  KNSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLT 124
           K+ GDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPV + E +CLT
Sbjct: 61  KHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVINGERQCLT 120

Query: 125 RDGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSN 184
            D QLMIIA VLRS +DLFY+LHMIFEFRTGFLPP+LPVFGSGELI+DPAAIAKRY+ SN
Sbjct: 121 IDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAKRYLFSN 180

Query: 185 FLIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRT 244
           FLIDILS+LPLPQLLVL I+PAA+GPVPLKTK++VKIAVLLQY+PRL RIYPLYREVTRT
Sbjct: 181 FLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLYREVTRT 240

Query: 245 SGILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCG 304
            GILTETAWSGAAFNLLIYMQ SHVVGAIWYLLS+E+QAKCW++AC+ +K C  + LYCG
Sbjct: 241 VGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDK-CKDNFLYCG 300

Query: 305 DHSKKIYPFIEKYCPPKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVG 364
           +  ++I P+I++YC PKESED KTFNFGMY+EAL+FQLT TTSFRRKFFYSFWWALRNV 
Sbjct: 301 NQRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWALRNVS 360

Query: 365 SSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHW 424
           SSGQNL                              QKYLQSATVK+EQMRINRRDAEHW
Sbjct: 361 SSGQNL------------------------------QKYLQSATVKVEQMRINRRDAEHW 420

Query: 425 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLF 484
           MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLKKVPLF
Sbjct: 421 MAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLKKVPLF 480

Query: 485 SSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPWSVT 544
           S M+KQLLD++CEYLKPVLFTEKSFILQEGD ID++LFIMKG LA I T GWKND  S  
Sbjct: 481 SGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKNDTLSGV 540

Query: 545 LKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNS 604
           L AGDFCGEELVQWAMDPTSTSLPIS+R VKSLTEVEAFALKANELKSVTSQFHFQ LNS
Sbjct: 541 LNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHFQHLNS 600

Query: 605 KQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRG-GGNGRFQDAL 664
           KQFQLSVR             FYSHQW VWAAYK+QEAW DYRERK+RG  G+GRFQDAL
Sbjct: 601 KQFQLSVR-------------FYSHQWNVWAAYKVQEAWQDYRERKRRGVRGDGRFQDAL 660

Query: 665 AETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQDRPNFT 724
           A+T GAS  FGATLYASIFISHLLQ VQRDQH +TTQLT+VMTLPPPPKPDDE D PNFT
Sbjct: 661 AKTAGASVGFGATLYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFT 679

Query: 725 ILN 726
           ILN
Sbjct: 721 ILN 679

BLAST of Spg031497 vs. TAIR 10
Match: AT5G53130.1 (cyclic nucleotide gated channel 1 )

HSP 1 Score: 688.7 bits (1776), Expect = 5.0e-198
Identity = 368/693 (53.10%), Postives = 492/693 (71.00%), Query Frame = 0

Query: 38  SILECIQRRFSSAS---ESFRRRVMNLGFKNSGDQSVKKPKFWKKILDPQKPFLQQWNKI 97
           SI +   R F S+S   + F+R   +  FK +  + +       KILDPQ PFLQ+WNKI
Sbjct: 42  SISDKFYRSFESSSARIKLFKRSYKSYSFKEAVSKGIGST---HKILDPQGPFLQRWNKI 101

Query: 98  FVLSSVIAVAVDPLFFYIPVFDKEHRCLTRDGQLMIIASVLRSFIDLFYLLHMIFEFRTG 157
           FVL+ +IAV++DPLFFY+P+ D   +CL  D ++ I ASVLRSF D+FY+LH+IF+FRTG
Sbjct: 102 FVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTG 161

Query: 158 FLPPTLPVFGSGELIKDPAAIAKRYISSNFLIDILSILPLPQLLVLVIVPAAKGPVPLKT 217
           F+ P+  VFG G L++D   IAKRY+SS+F+IDIL++LPLPQ+++L+I+P  +G   L T
Sbjct: 162 FIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLNT 221

Query: 218 KNVVKIAVLLQYIPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYMQASHVVGAIWY 277
           KN++K  V  QYIPR  RIYPLY+EVTRTSGILTETAW+GAAFNL +YM ASHV GA WY
Sbjct: 222 KNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFWY 281

Query: 278 LLSIEQQAKCWLDAC-KDNKDCIGDVLYCGDHSKKIYPFIEKYCPPKESEDDKTFNFGMY 337
           L SIE++  CW  AC ++N  CI  +LYC   +     F+ + C P ++ +   F+FG++
Sbjct: 282 LFSIERETVCWKQACERNNPPCISKLLYCDPETAGGNAFLNESC-PIQTPNTTLFDFGIF 341

Query: 338 LEALQFQLTDTTSFRRKFFYSFWWALRNVGSSGQNLQVSNHMGEVFFAVFIAILGLVLFA 397
           L+ALQ  + ++  F +KFFY FWW L+N+ S GQNL+ S ++ E+ FAVFI+I GLVLF+
Sbjct: 342 LDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLVLFS 401

Query: 398 FLISNIQKYLQSATVKIEQMRINRRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKE 457
           FLI N+Q YLQS T ++E+MR+ RRDAE WM+HR+LPE+LR+RIRRY+QYKWQ  RGV E
Sbjct: 402 FLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRGVDE 461

Query: 458 EELISNLPKDLRRDIKRHLCLALLKKVPLFSSMNKQLLDSVCEYLKPVLFTEKSFILQEG 517
           E L+SNLPKDLRRDIKRHLCLALL +VP+F  M++QLLD++C+ L+PVL+TE+S+I++EG
Sbjct: 462 ENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMDEQLLDALCDRLQPVLYTEESYIVREG 521

Query: 518 DQIDVMLFIMKGNLAAITTNGWKNDPW-SVTLKAGDFCGEELVQWAMDP-TSTSLPISTR 577
           D +D MLFIM+G L  ITTNG +     S  L AGDFCGEEL+ WA+DP +S++LPISTR
Sbjct: 522 DPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLGAGDFCGEELLTWALDPHSSSNLPISTR 581

Query: 578 KVKSLTEVEAFALKANELKSVTSQFHFQRLNSKQFQLSVRHVLTELMNLIYTLFYSHQWK 637
            V++L EVEAFALKA++LK V SQ  F+RL+SKQ + + R             +YS QWK
Sbjct: 582 TVRALMEVEAFALKADDLKFVASQ--FRRLHSKQLRHTFR-------------YYSQQWK 641

Query: 638 VWAAYKIQEAWHDYRERKKRGG---GNGRFQDALA-ETVGASASFGATLYASIFISHLLQ 697
            WAA  IQ AW  Y ++K          R QDALA E  G+S S GAT+YAS F +++L+
Sbjct: 642 TWAACFIQAAWRRYIKKKLEESLKEEENRLQDALAKEACGSSPSLGATIYASRFAANILR 701

Query: 698 AVQRDQHHQTTQLTR---VMTLPPPPKPDDEQD 718
            ++R    +  ++      M L  P +PD   D
Sbjct: 702 TIRRSGSVRKPRMPERMPPMLLQKPAEPDFNSD 715

BLAST of Spg031497 vs. TAIR 10
Match: AT1G01340.1 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 655.2 bits (1689), Expect = 6.1e-188
Identity = 342/656 (52.13%), Postives = 458/656 (69.82%), Query Frame = 0

Query: 78  KKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLTRDGQLMIIASVLRS 137
           K I++PQ  FLQ WNKIF+ + V+A+A+DPLFFYIP+ D    CLT D +L I AS+LR+
Sbjct: 61  KNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRT 120

Query: 138 FIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSNFLIDILSILPLPQL 197
            ID FY++H++F+FRT ++ P+  VFG GEL+ D  AIA +Y+SS F+ID+LSILPLPQ+
Sbjct: 121 LIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQI 180

Query: 198 LVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRTSGILTETAWSGAAF 257
           +VL ++P+   PV L TK+ +K +++ QY+PR+ R+YPLY EVTRTSGI+TETAW+GAA+
Sbjct: 181 VVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAW 240

Query: 258 NLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCGDHSKKIYPFIEKYC 317
           NL +YM ASHV GA+WYL+S+E++ +CW +AC+  K C    LYC +       F+   C
Sbjct: 241 NLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSC 300

Query: 318 P---PKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVGSSGQNLQVSNH 377
           P   P +  +   FNFG++ +AL+  + ++  F +KFFY FWW LRN+ + GQNLQ S  
Sbjct: 301 PFLDPGDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQNLQTSKF 360

Query: 378 MGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHWMAHRMLPEDLR 437
           +GE+ FA+ I I GLVLFA LI N+QKYL+S TV+ E+MR+ +RDAE WM+HRMLPEDLR
Sbjct: 361 VGEIIFAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLR 420

Query: 438 QRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMNKQLLDSV 497
           +RIRRY+QY+WQ  RGV+EE L+ NLPKDLRRDIKRHLCL LLKKVPLF  M++QLLD+V
Sbjct: 421 KRIRRYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAV 480

Query: 498 CEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPW-SVTLKAGDFCGEE 557
           C+ L+PVL+TE S++++EGD +  MLF+M+G L + TTNG ++  + +V LKA DFCGE+
Sbjct: 481 CDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGED 540

Query: 558 LVQWAMDPTSTS-LPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNSKQFQLSVRH 617
           L+ WA+DP S+S  PISTR V++LTEVEAFAL A +LKSV SQ  F+RL+SKQ Q + R 
Sbjct: 541 LLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQ--FRRLHSKQLQHTFR- 600

Query: 618 VLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRER---KKRGGGNGRFQDALAE----- 677
                       FYS QW+ W+   IQ AW  Y  R   K       R ++ALA      
Sbjct: 601 ------------FYSVQWRTWSVSFIQAAWRRYCRRKLAKSLRDEEDRLREALASQDKEH 660

Query: 678 ---TVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDEQD 718
              TV +S S G  LYAS F S+ L  ++    H  + L    TLP  P+   E D
Sbjct: 661 NAATVSSSLSLGGALYASRFASNALHNLR----HNISNLPPRYTLPLLPQKPTEPD 697

BLAST of Spg031497 vs. TAIR 10
Match: AT1G01340.2 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 653.7 bits (1685), Expect = 1.8e-187
Identity = 355/723 (49.10%), Postives = 481/723 (66.53%), Query Frame = 0

Query: 13  LRFGQPTSDFHDKYGGYQNSRLSPRSILECIQRRFSSASESFRRRVMNLGFKNSGDQSVK 72
           +RF         +YG  + +R S       + R F +    F++    L F +  D   K
Sbjct: 9   VRFKDEGKPLSSEYGYGRKARPS-------LDRVFKNVKWGFKK---PLSFPSHKDPDHK 68

Query: 73  KPK--FWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLTRDGQLMI 132
           +      K I++PQ  FLQ WNKIF+ + V+A+A+DPLFFYIP+ D    CLT D +L I
Sbjct: 69  ETSSVTRKNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEI 128

Query: 133 IASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSNFLIDILS 192
            AS+LR+ ID FY++H++F+FRT ++ P+  VFG GEL+ D  AIA +Y+SS F+ID+LS
Sbjct: 129 AASLLRTLIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLS 188

Query: 193 ILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRTSGILTET 252
           ILPLPQ++VL ++P+   PV L TK+ +K +++ QY+PR+ R+YPLY EVTRTSGI+TET
Sbjct: 189 ILPLPQIVVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTET 248

Query: 253 AWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCGDHSKKIY 312
           AW+GAA+NL +YM ASHV GA+WYL+S+E++ +CW +AC+  K C    LYC +      
Sbjct: 249 AWAGAAWNLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSN 308

Query: 313 PFIEKYCP---PKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVGSSGQ 372
            F+   CP   P +  +   FNFG++ +AL+  + ++  F +KFFY FWW LRN+ + GQ
Sbjct: 309 NFLTTSCPFLDPGDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQ 368

Query: 373 NLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHWMAHR 432
           NLQ S  +GE+ FA+ I I GLVLFA LI N+QKYL+S TV+ E+MR+ +RDAE WM+HR
Sbjct: 369 NLQTSKFVGEIIFAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHR 428

Query: 433 MLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMN 492
           MLPEDLR+RIRRY+QY+WQ  RGV+EE L+ NLPKDLRRDIKRHLCL LLKKVPLF  M+
Sbjct: 429 MLPEDLRKRIRRYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMD 488

Query: 493 KQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPW-SVTLKA 552
           +QLLD+VC+ L+PVL+TE S++++EGD +  MLF+M+G L + TTNG ++  + +V LKA
Sbjct: 489 EQLLDAVCDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKA 548

Query: 553 GDFCGEELVQWAMDPTSTS-LPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNSKQ 612
            DFCGE+L+ WA+DP S+S  PISTR V++LTEVEAFAL A +LKSV SQ  F+RL+SKQ
Sbjct: 549 SDFCGEDLLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQ--FRRLHSKQ 608

Query: 613 FQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRER---KKRGGGNGRFQDAL 672
            Q + R             FYS QW+ W+   IQ AW  Y  R   K       R ++AL
Sbjct: 609 LQHTFR-------------FYSVQWRTWSVSFIQAAWRRYCRRKLAKSLRDEEDRLREAL 668

Query: 673 AE--------TVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDD 718
           A         TV +S S G  LYAS F S+ L  ++    H  + L    TLP  P+   
Sbjct: 669 ASQDKEHNAATVSSSLSLGGALYASRFASNALHNLR----HNISNLPPRYTLPLLPQKPT 702

BLAST of Spg031497 vs. TAIR 10
Match: AT4G01010.1 (cyclic nucleotide-gated channel 13 )

HSP 1 Score: 644.0 bits (1660), Expect = 1.4e-184
Identity = 357/715 (49.93%), Postives = 478/715 (66.85%), Query Frame = 0

Query: 25  KYG-GYQNSRLSPRSILECIQRRFSSASESFRRRVMNLGFKNSGDQSVKKPKFWKKILDP 84
           +YG G   +R S  ++L+ ++R          ++ ++ G  N    S       K I++P
Sbjct: 20  EYGYGRNKARPSLNTVLKNVRRGL--------KKPLSFGSHNKKRDSNSSTTTQKNIINP 79

Query: 85  QKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLTRDGQLMIIASVLRSFIDLFY 144
           Q  FLQ WNKIF+ +SVIA+A+DPLFFYIP+ D E  CL     L I ASVLR+FID FY
Sbjct: 80  QGSFLQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFY 139

Query: 145 LLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSNFLIDILSILPLPQLLVLVIV 204
           ++H++F+FRT ++ P+  VFG GEL+ DP AIA +Y+SS F+ID+LSILPLPQL+VL ++
Sbjct: 140 IIHIVFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVI 199

Query: 205 PAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRTSGILTETAWSGAAFNLLIYM 264
           P    PV L TK+ +   +  QYIPR+ RIYPLY EVTRTSGI+TETAW+GAA+NL +YM
Sbjct: 200 PNVNKPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYM 259

Query: 265 QASHVVGAIWYLLSIEQQAKCWLDAC-KDNKDCIGDVLYCGDHSKKIYPFIEKYCP---P 324
            ASHV GA+WYL+S+E++ +CW +AC K  + C    LYC  +S     F+   CP   P
Sbjct: 260 LASHVFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTSCPFINP 319

Query: 325 KESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRNVGSSGQNLQVSNHMGEVF 384
            +  +   FNFG++ +AL+  + ++  F +KFFY FWW LRN+ + GQNL  S  +GE+ 
Sbjct: 320 DDITNSTVFNFGIFTDALKSGIVESDDFWKKFFYCFWWGLRNLSALGQNLNTSKFVGEII 379

Query: 385 FAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAEHWMAHRMLPEDLRQRIRR 444
           FAV I I GLVLFA LI N+QKYL+S TV+ E+MR+ +RDAE WM+HRMLP+DLR+RIRR
Sbjct: 380 FAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDDLRKRIRR 439

Query: 445 YDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMNKQLLDSVCEYLK 504
           Y+QYKWQ  RGV+EE L+ NLPKDLRRDIKRH CL LLKKVPLF  M++QLLD+VC+ LK
Sbjct: 440 YEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPLFEIMDEQLLDAVCDKLK 499

Query: 505 PVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPW-SVTLKAGDFCGEELVQWA 564
           PVL+TE S+ ++EGD ++ MLF+M+G L + TTNG +   + +V LK  DFCGE+L+ WA
Sbjct: 500 PVLYTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCGEDLLTWA 559

Query: 565 MDPTSTS-LPISTRKVKSLTEVEAFALKANELKSVTSQFHFQRLNSKQFQLSVRHVLTEL 624
           +DP S+S  PISTR V++LTEVEAFAL A++LK V SQ  F+RL+SKQ Q + R      
Sbjct: 560 LDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVASQ--FRRLHSKQLQHTFR------ 619

Query: 625 MNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRGG---GNGRFQDALAE---TVGASAS 684
                  FYS QW+ W A  IQ AW  +  RK          RF++A+ +      +S+S
Sbjct: 620 -------FYSVQWRTWGASFIQAAWRRHCRRKLARSLTEEEDRFRNAITKRERNAASSSS 679

Query: 685 FGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLP-PPPKPDDEQDRPNFTILN 726
             ATLYAS F S+ L+ +            R   LP  PPKP +    P+F++ N
Sbjct: 680 LVATLYASRFASNALRNL------------RTNNLPLLPPKPSE----PDFSLRN 695

BLAST of Spg031497 vs. TAIR 10
Match: AT2G46430.1 (cyclic nucleotide gated channel 3 )

HSP 1 Score: 626.7 bits (1615), Expect = 2.3e-179
Identity = 340/720 (47.22%), Postives = 473/720 (65.69%), Query Frame = 0

Query: 5   MGSQIPKSLRFGQPTSDFHDKYGGYQNSRLSPRSILECIQRRFSSASESFRRRVMNLGFK 64
           M  Q  K +RF     +F  K      +R S  S+++ ++R F   SE  R     L   
Sbjct: 2   MNPQRNKFVRFNGNDDEFSTK-----TTRPSVSSVMKTVRRSFEKGSEKIRTFKRPLSVH 61

Query: 65  NSGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVFDKEHRCLTR 124
           ++ ++   K K   ++++P   +LQ WNKIF+L SV+A+A DPLFFYIP    E  CL  
Sbjct: 62  SNKNKENNKKKKILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKPERFCLNL 121

Query: 125 DGQLMIIASVLRSFIDLFYLLHMIFEFRTGFLPPTLPVFGSGELIKDPAAIAKRYISSNF 184
           D +L  IA V R+FID FY++HM+F+F TGF+ P+   FG GEL +    IA RY+ S F
Sbjct: 122 DKKLQTIACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIALRYLGSYF 181

Query: 185 LIDILSILPLPQLLVLVIVPAAKGPVPLKTKNVVKIAVLLQYIPRLFRIYPLYREVTRTS 244
           LID+LSILP+PQ++VL IVP  + P  L  K ++K  +  QY+PR+ RIYPL++EVTRTS
Sbjct: 182 LIDLLSILPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLFKEVTRTS 241

Query: 245 GILTETAWSGAAFNLLIYMQASHVVGAIWYLLSIEQQAKCWLDACKDNKDCIGDVLYCGD 304
           G++TETAW+GAA NL +YM ASHV G+ WYL+SIE++ +CW +AC   ++C    LYC  
Sbjct: 242 GLVTETAWAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTHAYLYCSP 301

Query: 305 HSKKIYPFIEKYCP---PKESEDDKTFNFGMYLEALQFQLTDTTSFRRKFFYSFWWALRN 364
             +    F+   CP   P+E  +   FNFG++ +ALQ  + ++  F +KFFY FWW LRN
Sbjct: 302 TGEDNRLFLNGSCPLIDPEEITNSTVFNFGIFADALQSGVVESRDFPKKFFYCFWWGLRN 361

Query: 365 VGSSGQNLQVSNHMGEVFFAVFIAILGLVLFAFLISNIQKYLQSATVKIEQMRINRRDAE 424
           + + GQNL+ S   GE+ FA+ I I GLVLFA LI N+QKYLQS TV++E+MR+ RRDAE
Sbjct: 362 LSALGQNLKTSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMRVKRRDAE 421

Query: 425 HWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVP 484
            WM+HRMLP+DLR+RIR+Y+QYKWQ  +GV+EE L+S+LPKDLR+DIKRHLCL LLKKVP
Sbjct: 422 QWMSHRMLPDDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLCLKLLKKVP 481

Query: 485 LFSSMNKQLLDSVCEYLKPVLFTEKSFILQEGDQIDVMLFIMKGNLAAITTNGWKNDPW- 544
            F +M+ +LLD++C  LK VL+TEKS+I++EG+ ++ MLFIM+GNL + TT G +   + 
Sbjct: 482 WFQAMDDRLLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYGGRTGFFN 541

Query: 545 SVTLKAGDFCGEELVQWAMDPTSTSLPISTRKVKSLTEVEAFALKANELKSVTSQFHFQR 604
           SV L AGDFCG +L+ WA+DP S+  PIS+R V++LTEVE F L A++LK V +Q  ++R
Sbjct: 542 SVDLVAGDFCG-DLLTWALDPLSSQFPISSRTVQALTEVEGFLLSADDLKFVATQ--YRR 601

Query: 605 LNSKQFQLSVRHVLTELMNLIYTLFYSHQWKVWAAYKIQEAWHDYRERKKRGG---GNGR 664
           L+SKQ    +RH+           FYS QW+ WAA  IQ AW  +  RK         G+
Sbjct: 602 LHSKQ----LRHMFR---------FYSVQWQTWAACFIQAAWKRHCRRKLSKALREEEGK 661

Query: 665 FQDALA--ETVGASASFGATLYASIFISHLLQAVQRDQHHQTTQLTRVMTLPPPPKPDDE 716
             + L   ++ G   + GA +YAS F SH L+ ++ +   + ++   ++TL P    D E
Sbjct: 662 LHNTLQNDDSGGNKLNLGAAIYASRFASHALRNLRANAAARNSRFPHMLTLLPQKPADPE 700