Spg031480 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031480
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionDEAD-box ATP-dependent RNA helicase 50
Locationscaffold11: 36355016 .. 36362898 (+)
RNA-Seq ExpressionSpg031480
SyntenySpg031480
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATGATATTTGTCCACTTCGAGCATTAGCCCCCATGACTTTGCTTTTGGTTTCACCCAAAAGGTCTCATAGCAATAGAGATAGTTGTCCTCACTTATATACCCATGATCTTCTTCTTGTCTAGCCAATGTAGGACTTTGTTTGCATTCCCAACAATCCTCCCCTTGAACAAAGTATCACCGAACCTCCTCTCAAATGGTCATCTGTCCATCTAGCTCTAATCTAGGCTGACTCCTTTTCTCTGGAGTACATCGCCTGATAGAGCTCCACCATGGATCACATTGTGGGTGGAGCACATTGTGGGTCATAGGTGTTGACCACTATTTATTGCACCCTTAGCACAAATTTAGAGTTTTCGGTTATGTATTAAATGGTTGATTTAATATCCAAGTTTCTCTTTGATCTTCATATTTACATTTTGGAAGACCAAAAAGTAATGTCAAGGCATCTGGTGGGTGTTTGGTTTCAAGTAAATAGCATTAGAGAAAATGTAATGCCATTCTCATCTCTATCTATTTCATTTTGGTTGATCTTCATTTTAATTTTTTGTCATTCTCTTTCCCGTTAAGTAAACGGGACCAGATGGGCTTGGAATGGACAAAAGATTCAGTTTTACATGATGCATGGGATACTAAAGTAATATAGTGATAGATTGCTTTATTCAGTATATAAATTTCATAACTAGAAAAGAAGTATCTTATTGCTCACTAACAAATTTGTTGCAAAGGGTTATAAACTTATAATAAAAGAAATTATCACAGTGCTTATATACTTCTATATGAATAATGAATAATGGCAGGCCAAGGCATTTGCATCAGTTATTGAAGGAAAGAGTTGTATCATTTCTGATCAAAGTGGATCTGGTAAGACATTAGCATATCTTGCACCATTAATCCAGCGTCTGAGGCAGGAAGAACTCGAAGGACTTCGAAAATCTTCTTCACAAAGTCCCCAAATTGTTATCATTGTGCCAACAGCAGAATTGGCTTCTCAGGTGGATTATTCTTGTTCCGTATTCTAACTGTGCTTCACAGTGTTCTTCCAATTGCACATTTCTGATACATTTTTTAGCGTATAGTTTTACTTTCTGTTTACTTCAAAAAATCCGCTTTTGTGTGGATGCAAAATGAAGAATTAGCTTCAAAAAATAAATTTTCTGTCATGAATTGATCAGATACTGCTTCAGATTAATGAGTGTTTTGCATCAACCAAGACCAATGAATTTTCTGTAGTAGAAATCTATTACTGGAGAACTACTTTACATACTTGAATCGATAACTCTCTCATGCTATGGAATGATGGGGAGAAGACTATTCTGCATTTGCTCATGGCTATAGTTTAGTGTCTTTTTCTTTTTTTGCCAACATGTAGGGGTGAGGGGATTGAACCTCTGACCTCTTGGTCAGAATGCAAGCTTCATGCCAGTTGAGCCATGCTCTTGTTGGTGCTAAAGTTTAGTGTATTTGACTATGGTCTTGTGGTTAGAAAATCACATTTTTTACTTTTTTGGGGTGTTTTACATCTCTCTTTTTCTGATTGTATGAGACTGGATGTCATGCAAGTACATCGCGTCGGTGTACTTGATGGGCAGTTTAGACAACTATAACCTTGACCTACATTAGGAGGGAAGGGATAAAGAATGGATTGTTATCATTCAAACATTAGGTATGATTAAAACCTATAAAGCTATAATTAGCCTCTAATATAAACCTTATGTGCTAAGGGAAATGGAAAAAGAATATTAGCATATCTAGTAACTTAAAAGGGGCTCATAAAGAACTAAATCACAGAAAAAGACCCTGCAGGCACCAACGGCCAATTTTGCATCATTTATAATTTGGAGAATTTATGCTGGTATTTTTCTTCATTGGGTGGATAATGTTTGACAACAATGCGAATTTCACAGGTATTACAGAATTGCCGATCCATTTCAAAATTTGGGGTGCCATTTAGGTCTATGGTTGTGACAGGTGGTTTCCGGCAGAGGACTCAACTTGAAAATCTACAGGAAGGTGTTGATGTTCTAATAGCAACACCTGGTCGTTTGATGATTCTAATAAATGAAGGCTTCTTGCAGTTATCAAATCTAAGATGGTAATCCTGTTTTTCTAAGAAGTTTTTTATTACTTTCCATTGAGTCGCCTTTTATTTCTAATGGTGGGTCCTAATTTATTTTGACATAGTAAGAATGTTTTCTTAAACTTGATTAAGCATTCTTTTTTTTCAAGATTTTACATCTACATATTACATCAAAACTGAAGTATTTTTGCTAGAGATGCATAATTACTAGATTTATCAATGTCTTTTGGAACCATGTTGTTGATTTGTCCAAGTGTGGAAAACTATGTATATTAAACGACAAATGCAATGCTGAGATCCATTGTCCAGGCATTGGCCAACTCAGGATTAGATACTCTTAACTACCAAAGCCTCACATTGAATATTTATTTTAAGTTTCAATTAAGTTTAAAGTTGACTATAAGTCTCTTTAATTTTCATCAATTATCCATCTGAAAATTTGCATAACATTTATATAAAAGCACATGATTAATTCACAGCAGCAGAAAACTGTTCTAATCTTTGATATATGTATCTTGATTTTTAGTTTTCATGGTGGGGTTCAATCCTGCATATAAGCAGTACTGTTTATATTATGATTAAGTTAAATATCTGTATCCTGTGCTTGTTTGCTGAAAATCAAAGTATTATTGTGAAGTAGTGAATAATTTGTGCAGTTTCAGCCCCTCTAGCAGGGTCATTGTATGTTTTTATGCCTATAGGAAGACTTTGTATATTTTATTTGGATATATGGAACAGAGGAGACATACATATTAGAGTAAAAAAAGGTTGGGAAAGGACTTTTCAAAAAAAAAGAAAAAAGGTTGGGGAAGGAGGGACAAAAAATATAGGATACATGTCTGATGTCTCAGGAAGGACTTTGAGTTGGATGTCATAAAATACTTCAATATTCTGTTCGATATTGTTATTCAGATAATGAATCAGTTATTGCCTCTTAGTTTCTGACCTTTATCCATATTAATAACCCAGCTGTATGCTGGTTTGATTGTGAGTATTAGTGCTCTGGACTTCATTGAAATTACCGTTGCTCTTTCTATTCCCATAATGAATTATTTCCTACTTTCTGCTATAAGAGTTATTTATATGACGTTGTTCCTTTACTGATAAACAACCTTGTGCTATAGTGCTGTAATGGACGAGGTAGATATCCTTTTTAATGATGAGGACTTTGAAGGTGCACTGCGGACTTTAATGAAATCTGCACCTGTTAACACCCAATATCTATTTGTGACTGCAACTTTACCAGTTGACATATACAATACACTAGTAGAAAATTTTCCTGATTGTGAAGTGATCATGGGACCTGGAGTTCACCGGATAAGTCCTTTCCTTGAAGAGGTTGGTCTTCAATCAATATCAGTTTATCTCTTTTATACTTTTCCTGAGGTGATCAGTGATGGATGGACTATAATGCTGTTTTATTGATACTACATATGTACTAGCATATTGGAAATTGGTCCATTCTCGAGTAAGTTTTATTTTTAGTACAAACCATGCATTTTGCTATTATAAATGTTAGGTCTTTTGAACTTAAGGTGGCCTAGCAATGTCTCAGAAGTATAGATAGAAAATTCTAATCTATACACAGACCATTCGGAAGGGATGAAGGTGGAGAAGGGGGAGATGCGGTAGGGAGGAGGGAAGAGGAGAGAGAGTGGTGGGGCATGAGTAGTTTTAGTTTATTTTTTCTTCTTCAAATCATGCAATTTGTTTGGTATAAATGTTCGATCACTTCAACGTAAGGTGGCTCACCAATGTCTCAGTACTTGATATGCTTACTATTAGGATATAAATAGAAAATTCTAATCTATTTATGGACCACTCTAATTTGACTCATTCATGTTTTTCTCATCCAAGAAATATAGAATGATATTTTGCCTTTTCCTGTTAGTGTAAAGTGACGAGATTTATTATTTTTTCTCCTCTTCTTTGCTTGGGCTGTCGACACTACTGAACCATGACAATCAAATTGCAAATCCAGGTTCTTGTAGATTGCAGTGGTGAAGACGAGCAGAACAAAACACCGGATGCTGCTTTCTCGAATAAAAAAGATGCCCTCCTGCAGATTGCAGAAGGAACTCCAGTCTCAAAAACTATTGTTTTCTGTAACAAGGTATATTCAATTTTGGTTTAGGCTCTGGGATATTCCTAAGTTCCTGTATTGTGCGCATTTAAATTTTCCACCATTTACCAGTAATTGAAAGGGCTTCCTTAGTGAGGACTATACAGGGATGCGAAGATGTCAACTTTAAACTTGTTTGGTTTTGCAAGTCTGGGCTCTTTGTTACCTTTTGATTTTTGAGTTTCCTTGCAATGTAGCTAATGAAACTAGAAGAGCAGACAAACATATTTTGTAGGAGGTGGTGGATGACAGCGAAAAAGTTTTCATATTTTGAAGATTAGGAAAGAAGAGCAGTAGAGTGATGAAATTTATTTATTATTTTTTTTCAAATAAAGCAATTTAGGTAAATGTTGAGGAGACTTTCAAATGAGCCATAATCTCTTTGAGTTTCCAATGAAATTCAAAATAATGACCGAGTTAATAGATTGTCATGGGGGCTATTAGATAACATGAGTTCCATCTCAAAACCAATTGGCAATGAAAGAAGTAGCCTATGTATCTTATAAATTGTGAGGTCTCCACCCATCTTTTCAATGTGAGATCCTCAACATGCTCCCTCGAGATGGTGCCTCTTTGGGTTCACCATTCTTGGATCAGATCCCTTATTCTTTTCATTTGGTCCAAATACCTGTTTGGGTTTTTTGGGCTCTGATATCATATTAGATAACATGAGTTTCATCTCAAAACTAATTGGCGAAAGGAGTAGCTCATGTATCTTATGAATTGTGAGGTTTCCACTCATCTTTTCAATATGGGATCCTCAATAGGGGTGCATAGGGAATTTTGCATAAATATATTTCTCAAGGTATTTTGAATCCTTGGTAGAAAATTGTTTTTGGTAATGTTTTAAGAATTCTGTGGGAATAATTTTTGGATTAAGCTTCAAAAGGGAAGTGTTGGAATTGTGTCATAATAAAGATCTTCTCAATTAATGCAGCAAAATACAAGAAAGTGGCCCCTGGGATAATGCGAGAGACCCCAAATTCTTCCTTTTCTTGAGACTCCAAAATCTACCTTTTAACCATTACATCGTCCATGTGTGTTTCTCTCTTTCCCTAACTAACTTATTCCTGACATAACTCTTCTTGTTCTCTACCCCTATCAGATGTAACTACTAAGAGAGTAAAAGACCCCGCATTACAAGTCAGTTTCCGTATTACTCTCAAGGTTCATCCTTCCTTTTCTTCTATGGTGTACTTGGAGCATGTGGGGTCAACCATGTCCTTTCAACTACTTGGGTGACTAGAATGAAACAAACGGATTTTTGGAAGATAAATTTGATGACTATGAGTTAATTTGGATTAAAGTACTACTGTAATTATTAAGTAAAATACTAGAATAGTGGCCTCATTCTTTTTTCTATTTTTTGTAAGTTTGTTTTGCCGATGTAGTTTTTCATGTAATTTATTTTTCTTTTTTGAGAATGCTTAATATTCTTATTCTGTTTCCATTTTCAATCAATATAGTTTTGTTTTTGACCAAGGAAAAAAAACTAACGAACAGAAAAATTCCAATTTTTCCGAAGTTCCTACTCTTATAGTAGCTTATTCGTTGAATTTTGCAGATTGAGACATGTAGAAGAGTTGAAAATGCATTGCAGCGTTTTGATAAAAAAGGTAGCCATTTGCAAGTCTTTCCGTTTCATGCTGCCCTAGCAAGAGAGTCGCGGCTTGCAAATATGGAAGCATTCACCAATTCCCATTCTAACCAAGTATCCCAGTTCTTGGTTTGCACTGATAGGTACTTCTTACTATTCCAAAACGTACTACAAAATAGTGACAAAATTCACTGAATTGATAAGAGCTTTCTTCCTGCCCTTAAATTACAACCAATTTCCATTAGACGTTGTTTGATAGAATACACGGTTCAACTCATGGAATCCCAAAAGACTGAGTCCTTTGTTCCACGAGAAACCAGCATAGGTCAACACAGACACAAATGATCCGCATCTTTCCAATCACAATCGCACGTATAACAAGGAAAATTTGTTAGCAACATATGATTTGATATTTATTCTCTTACGAGTTTGATTCCAAATCAGCACCTGAACCATTTTAGAACTTTTTCCGATCAAATGAACCTAAGAAGAGCTATTTCTGGAGCCATATTATACGACAAAAGATATAGGAATGACCCACTGGAAAAACTGCATGTAGGACTCTAGATTATTTTTTGGTAAGAACAACTCAAGATGAAACCCTTTAAAATGGACAAATAATTAATAAGATTAAAGTTTTCCTTTCATTCAAATTTCAAAAGAATTAAAATGTCAGCATGTTAGAAATACTATCAGGGTATACAGTAGAATTAGCTAAGGAGTTTGTTAGTCATTATAGTAGGTTAAAGCAGCTTAAGGCAAGCAGATCTTAAGAATGGTTTGTGGAACAATCCAACCCATCTTGAAAATTTGTAATTTTCCTTTCTTATAGGCTATAGTAATAGCATTTTCTATTTATTTTTACTCCACTTTCGGGTCCCTGTCACAATGGTTCTAGTTTCCGTCTACTTTTTATCAACTTCTATTGCGATTTGCGAAATCTTGTCTCCCGTAAACCACTAAAGAAACAGTTGGTCTTGTTTAACTTGGATACATAATTTGAATAGAGTTTTTATGGGAAAAGTAAAGAATAAGTATTACATTCTGATACTGATGGGCCTCCGGTGCATTTTACTTATGCAAGGAGAAAAATATAGTTGGTTTTGTTGTATCACATATTTGCAATATGGACATGTACTACTCTATGCTGTTCAGTAATCAGTTGGTTGTGTTTGTTAGGAGTTGCTCAAAAGTTCGTCAAGAGTTGTTAGAAATGTGAATCAGTTTAGTTTGGTTTTATACCAAACCAACTGAATGGATTGGTTCAATCTCATGGAACACACAAACCTAGATAAATCAGTTGTAAATATGAATTTCTGAGAACTAATGTCATCTAACAAGTTAATATTTGGTTTATTTAAGCCCTATGTTGCTAACACCCTGTAAATGGGAGTTTACTTTTAAGATCATATATCAGACACCATGAAAACACGAAATGAATATGAACTTCCTGATCAGAAGACTTCAATTTTGATAGAAGGATGTACAATGAGCATAATGATTTGAGTTTGAACATCTAAAGAACAGGAAATTGTTGTAAGAAATGAATCTTTTGAAATTATTGACAGGGCATCACGTGGAATTGATTTTCCAAATGTGGATCACGTCATACTCTTCGACTTCCCCCGTGATCCTAGCGAGTATGTTCGCCGTGTTGGAAGAACTGCCAGAGGTGCTACAGGTAAAGGAAAAGCATTCATCTTCGTGGTCGGAAAACAGGTATCCCTTGCGCGGAGGATAGTTGAAAGAAACAGGAAAGGTCACCCGTTACATGATGTACCATCTGCTTATGAGCTTACATACTAAATAACGGCTTGATAAAAGAAAAGCAGATGCAATTACTGGAAGATGTTGCTGAATTGACTAGCTGGAAATTCAGCTTAATGGAATGGAGTTGCCTCTCCTGCTATCAGATAATAAGGTTTTATTCTCTCTCTCTCTCATATTAATCTCTCTGAATGTGTCAAGGGACTGACAAATGCATTAGTAAATTTCAGCTTAGTCTTATCGTCTCTTAGGGTCCGTTTGGAATGCCTTTTAGTTTAAATGTTTTTCAAGAATGCATTTTATTTTAAATATTTT

mRNA sequence

ATGGCCAAGGCATTTGCATCAGTTATTGAAGGAAAGAGTTGTATCATTTCTGATCAAAGTGGATCTGGTAAGACATTAGCATATCTTGCACCATTAATCCAGCGTCTGAGGCAGGAAGAACTCGAAGGACTTCGAAAATCTTCTTCACAAAGTCCCCAAATTGTTATCATTGTGCCAACAGCAGAATTGGCTTCTCAGGTATTACAGAATTGCCGATCCATTTCAAAATTTGGGGTGCCATTTAGGTCTATGGTTGTGACAGGTGGTTTCCGGCAGAGGACTCAACTTGAAAATCTACAGGAAGGTGTTGATGTTCTAATAGCAACACCTGGTCGTTTGATGATTCTAATAAATGAAGGCTTCTTGCAGTTATCAAATCTAAGATGTGCTGTAATGGACGAGGTAGATATCCTTTTTAATGATGAGGACTTTGAAGGTGCACTGCGGACTTTAATGAAATCTGCACCTGTTAACACCCAATATCTATTTGTGACTGCAACTTTACCAGTTGACATATACAATACACTAGTAGAAAATTTTCCTGATTGTGAAGTGATCATGGGACCTGGAGTTCACCGGATAAGTCCTTTCCTTGAAGAGGTTCTTGTAGATTGCAGTGGTGAAGACGAGCAGAACAAAACACCGGATGCTGCTTTCTCGAATAAAAAAGATGCCCTCCTGCAGATTGCAGAAGGAACTCCAGTCTCAAAAACTATTGTTTTCTGTAACAAGATTGAGACATGTAGAAGAGTTGAAAATGCATTGCAGCGTTTTGATAAAAAAGGTAGCCATTTGCAAGTCTTTCCGTTTCATGCTGCCCTAGCAAGAGAGTCGCGGCTTGCAAATATGGAAGCATTCACCAATTCCCATTCTAACCAAGTATCCCAGTTCTTGGTTTGCACTGATAGGGCATCACGTGGAATTGATTTTCCAAATGTGGATCACGTCATACTCTTCGACTTCCCCCGTGATCCTAGCGAGTATGTTCGCCGTGTTGGAAGAACTGCCAGAGGTGCTACAGGTAAAGGAAAAGCATTCATCTTCGTGGTCGGAAAACAGGTATCCCTTGCGCGGAGGATAGTTGAAAGAAACAGGAAAGGTCACCCGTTACATGATGTACCATCTGCTTATGAGCTTACATACTAA

Coding sequence (CDS)

ATGGCCAAGGCATTTGCATCAGTTATTGAAGGAAAGAGTTGTATCATTTCTGATCAAAGTGGATCTGGTAAGACATTAGCATATCTTGCACCATTAATCCAGCGTCTGAGGCAGGAAGAACTCGAAGGACTTCGAAAATCTTCTTCACAAAGTCCCCAAATTGTTATCATTGTGCCAACAGCAGAATTGGCTTCTCAGGTATTACAGAATTGCCGATCCATTTCAAAATTTGGGGTGCCATTTAGGTCTATGGTTGTGACAGGTGGTTTCCGGCAGAGGACTCAACTTGAAAATCTACAGGAAGGTGTTGATGTTCTAATAGCAACACCTGGTCGTTTGATGATTCTAATAAATGAAGGCTTCTTGCAGTTATCAAATCTAAGATGTGCTGTAATGGACGAGGTAGATATCCTTTTTAATGATGAGGACTTTGAAGGTGCACTGCGGACTTTAATGAAATCTGCACCTGTTAACACCCAATATCTATTTGTGACTGCAACTTTACCAGTTGACATATACAATACACTAGTAGAAAATTTTCCTGATTGTGAAGTGATCATGGGACCTGGAGTTCACCGGATAAGTCCTTTCCTTGAAGAGGTTCTTGTAGATTGCAGTGGTGAAGACGAGCAGAACAAAACACCGGATGCTGCTTTCTCGAATAAAAAAGATGCCCTCCTGCAGATTGCAGAAGGAACTCCAGTCTCAAAAACTATTGTTTTCTGTAACAAGATTGAGACATGTAGAAGAGTTGAAAATGCATTGCAGCGTTTTGATAAAAAAGGTAGCCATTTGCAAGTCTTTCCGTTTCATGCTGCCCTAGCAAGAGAGTCGCGGCTTGCAAATATGGAAGCATTCACCAATTCCCATTCTAACCAAGTATCCCAGTTCTTGGTTTGCACTGATAGGGCATCACGTGGAATTGATTTTCCAAATGTGGATCACGTCATACTCTTCGACTTCCCCCGTGATCCTAGCGAGTATGTTCGCCGTGTTGGAAGAACTGCCAGAGGTGCTACAGGTAAAGGAAAAGCATTCATCTTCGTGGTCGGAAAACAGGTATCCCTTGCGCGGAGGATAGTTGAAAGAAACAGGAAAGGTCACCCGTTACATGATGTACCATCTGCTTATGAGCTTACATACTAA

Protein sequence

MAKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTAELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGFLQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFPDCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVFCNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCTDRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIVERNRKGHPLHDVPSAYELTY
Homology
BLAST of Spg031480 vs. NCBI nr
Match: XP_008439020.1 (PREDICTED: DEAD-box ATP-dependent RNA helicase 50 [Cucumis melo] >KAA0052139.1 DEAD-box ATP-dependent RNA helicase 50 [Cucumis melo var. makuwa] >TYK05106.1 DEAD-box ATP-dependent RNA helicase 50 [Cucumis melo var. makuwa])

HSP 1 Score: 724.5 bits (1869), Expect = 4.6e-205
Identity = 363/380 (95.53%), Postives = 372/380 (97.89%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFASVIEGKSCIISDQSGSGKTLAYL PLIQRLRQEELEG+RKSSS+SPQIVIIVPTA
Sbjct: 269 AKAFASVIEGKSCIISDQSGSGKTLAYLVPLIQRLRQEELEGVRKSSSKSPQIVIIVPTA 328

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQL+NLQEGVDVLIATPGRLM+LINEGF
Sbjct: 329 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLDNLQEGVDVLIATPGRLMLLINEGF 388

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           L LSNLRCAVMDEVDILFNDEDFE ALR+LMKSAPVNTQYLFVTATLPVDIYNTLVENFP
Sbjct: 389 LLLSNLRCAVMDEVDILFNDEDFEVALRSLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 448

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSGEDEQNKTPDAAF NKKDALLQIAEGTPVSKTIVF
Sbjct: 449 DCEVIMGPGVHRISPSLEEVLVDCSGEDEQNKTPDAAFLNKKDALLQIAEGTPVSKTIVF 508

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCR+VENALQRFDKKGS LQVFPFHAALARESRLANMEAFTNSHSNQVS+FLVCT
Sbjct: 509 CNKIETCRKVENALQRFDKKGSRLQVFPFHAALARESRLANMEAFTNSHSNQVSKFLVCT 568

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQV LARRI+
Sbjct: 569 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVPLARRII 628

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 629 ERNRKGHPLHDVPSAYELTY 648

BLAST of Spg031480 vs. NCBI nr
Match: XP_022979728.1 (DEAD-box ATP-dependent RNA helicase 50 isoform X2 [Cucurbita maxima])

HSP 1 Score: 722.2 bits (1863), Expect = 2.3e-204
Identity = 362/380 (95.26%), Postives = 371/380 (97.63%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEEL+GL+K SSQSPQIVIIVPTA
Sbjct: 265 AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELQGLQKPSSQSPQIVIIVPTA 324

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQL+NLQEGVDVLIATPGRLM+LINEGF
Sbjct: 325 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLDNLQEGVDVLIATPGRLMLLINEGF 384

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           LQLSNLRCAVMDEVDILFNDEDFE AL+TLMKSAPVN QYLFVTATLPVDIYNTLVE FP
Sbjct: 385 LQLSNLRCAVMDEVDILFNDEDFEVALQTLMKSAPVNAQYLFVTATLPVDIYNTLVEKFP 444

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSGEDEQNKTPDAAF NKKDALLQIAEGTPV KTIVF
Sbjct: 445 DCEVIMGPGVHRISPSLEEVLVDCSGEDEQNKTPDAAFLNKKDALLQIAEGTPVLKTIVF 504

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCRRVENALQRFDKKGS LQVFPFHAALAR+SRLANMEAFTNSHSNQVS+FLVCT
Sbjct: 505 CNKIETCRRVENALQRFDKKGSRLQVFPFHAALARDSRLANMEAFTNSHSNQVSKFLVCT 564

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRI+
Sbjct: 565 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRII 624

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 625 ERNRKGHPLHDVPSAYELTY 644

BLAST of Spg031480 vs. NCBI nr
Match: XP_022979726.1 (DEAD-box ATP-dependent RNA helicase 50 isoform X1 [Cucurbita maxima] >XP_022979727.1 DEAD-box ATP-dependent RNA helicase 50 isoform X1 [Cucurbita maxima])

HSP 1 Score: 722.2 bits (1863), Expect = 2.3e-204
Identity = 362/380 (95.26%), Postives = 371/380 (97.63%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEEL+GL+K SSQSPQIVIIVPTA
Sbjct: 270 AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELQGLQKPSSQSPQIVIIVPTA 329

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQL+NLQEGVDVLIATPGRLM+LINEGF
Sbjct: 330 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLDNLQEGVDVLIATPGRLMLLINEGF 389

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           LQLSNLRCAVMDEVDILFNDEDFE AL+TLMKSAPVN QYLFVTATLPVDIYNTLVE FP
Sbjct: 390 LQLSNLRCAVMDEVDILFNDEDFEVALQTLMKSAPVNAQYLFVTATLPVDIYNTLVEKFP 449

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSGEDEQNKTPDAAF NKKDALLQIAEGTPV KTIVF
Sbjct: 450 DCEVIMGPGVHRISPSLEEVLVDCSGEDEQNKTPDAAFLNKKDALLQIAEGTPVLKTIVF 509

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCRRVENALQRFDKKGS LQVFPFHAALAR+SRLANMEAFTNSHSNQVS+FLVCT
Sbjct: 510 CNKIETCRRVENALQRFDKKGSRLQVFPFHAALARDSRLANMEAFTNSHSNQVSKFLVCT 569

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRI+
Sbjct: 570 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRII 629

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 630 ERNRKGHPLHDVPSAYELTY 649

BLAST of Spg031480 vs. NCBI nr
Match: XP_022137867.1 (DEAD-box ATP-dependent RNA helicase 50 [Momordica charantia])

HSP 1 Score: 720.7 bits (1859), Expect = 6.6e-204
Identity = 362/380 (95.26%), Postives = 371/380 (97.63%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFA VIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQ+PQIVIIVPTA
Sbjct: 259 AKAFAPVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQNPQIVIIVPTA 318

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQLENLQEGVDVLIATPGRLM+LINEGF
Sbjct: 319 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLENLQEGVDVLIATPGRLMLLINEGF 378

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           L+LSNLRCAVMDEVDILFNDEDFE AL+TLMKSAPVNTQYLFVTATLPV+IYNTLVENFP
Sbjct: 379 LKLSNLRCAVMDEVDILFNDEDFEVALQTLMKSAPVNTQYLFVTATLPVEIYNTLVENFP 438

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSG DEQNKTPDAAF NKKDALLQIAE TPV +TIVF
Sbjct: 439 DCEVIMGPGVHRISPCLEEVLVDCSGVDEQNKTPDAAFLNKKDALLQIAEQTPVLRTIVF 498

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNS SNQVSQFLVCT
Sbjct: 499 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSQSNQVSQFLVCT 558

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQV+LARRI+
Sbjct: 559 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVTLARRII 618

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 619 ERNRKGHPLHDVPSAYELTY 638

BLAST of Spg031480 vs. NCBI nr
Match: XP_023528025.1 (DEAD-box ATP-dependent RNA helicase 50 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 720.3 bits (1858), Expect = 8.7e-204
Identity = 361/380 (95.00%), Postives = 370/380 (97.37%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEEL+GL+KSS QSPQIVIIVPTA
Sbjct: 270 AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELQGLQKSSPQSPQIVIIVPTA 329

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQL+NLQEGVDVLIATPGRLM+LINEGF
Sbjct: 330 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLDNLQEGVDVLIATPGRLMLLINEGF 389

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           LQLSNLRCAVMDEVDILFNDEDFE AL+TLMKSAPVN QYLFVTATLPVDIYNTLVE FP
Sbjct: 390 LQLSNLRCAVMDEVDILFNDEDFEVALQTLMKSAPVNAQYLFVTATLPVDIYNTLVEKFP 449

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSGEDEQNKTPDAAF NKKDALLQIAEGTPV KTIVF
Sbjct: 450 DCEVIMGPGVHRISPSLEEVLVDCSGEDEQNKTPDAAFLNKKDALLQIAEGTPVLKTIVF 509

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCRRVENALQRFDKKGS LQVFPFHAALAR+SRLANMEAFTN HSNQVS+FLVCT
Sbjct: 510 CNKIETCRRVENALQRFDKKGSRLQVFPFHAALARDSRLANMEAFTNPHSNQVSKFLVCT 569

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRI+
Sbjct: 570 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRII 629

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 630 ERNRKGHPLHDVPSAYELTY 649

BLAST of Spg031480 vs. ExPASy Swiss-Prot
Match: Q8GUG7 (DEAD-box ATP-dependent RNA helicase 50 OS=Arabidopsis thaliana OX=3702 GN=RH50 PE=2 SV=2)

HSP 1 Score: 579.3 bits (1492), Expect = 3.1e-164
Identity = 282/379 (74.41%), Postives = 322/379 (84.96%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           A AF+ VI+GKSCII+DQSGSGKTLAYL P+IQRLR+EEL+G  KSS   P+++++VPTA
Sbjct: 402 AMAFSPVIDGKSCIIADQSGSGKTLAYLVPVIQRLREEELQGHSKSSPGCPRVIVLVPTA 461

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISK GVPFRSMVVTGGFRQRTQLENL++GVDVLIATPGR   L+NEG 
Sbjct: 462 ELASQVLANCRSISKSGVPFRSMVVTGGFRQRTQLENLEQGVDVLIATPGRFTYLMNEGI 521

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           L LSNLRCA++DEVDILF D++FE AL+ L+ S+PV  QYLFVTATLP++IYN LVE FP
Sbjct: 522 LGLSNLRCAILDEVDILFGDDEFEAALQNLINSSPVTAQYLFVTATLPLEIYNKLVEVFP 581

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEV+MGP VHR+S  LEE LVDCSG+D   KTP+ AF NKK ALLQI E  PVSKTI+F
Sbjct: 582 DCEVVMGPRVHRVSNALEEFLVDCSGDDNAEKTPETAFQNKKTALLQIMEENPVSKTIIF 641

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCR+VEN  +R D+K   L V PFHAAL++ESRL NM+ FT+S   + S FLVCT
Sbjct: 642 CNKIETCRKVENIFKRVDRKERQLHVLPFHAALSQESRLTNMQEFTSSQPEENSLFLVCT 701

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDF  VDHV+LFDFPRDPSEYVRRVGRTARGA GKGKAFIFVVGKQV LARRI+
Sbjct: 702 DRASRGIDFSGVDHVVLFDFPRDPSEYVRRVGRTARGARGKGKAFIFVVGKQVGLARRII 761

Query: 362 ERNRKGHPLHDVPSAYELT 381
           ERN KGHP+HDVP+AYE T
Sbjct: 762 ERNEKGHPVHDVPNAYEFT 780

BLAST of Spg031480 vs. ExPASy Swiss-Prot
Match: Q0DVX2 (DEAD-box ATP-dependent RNA helicase 50 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0108600 PE=2 SV=2)

HSP 1 Score: 546.2 bits (1406), Expect = 2.9e-154
Identity = 260/373 (69.71%), Postives = 315/373 (84.45%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           A A+  V+EGKSCII DQSGSGKTLAYL P++Q LR+EE+EGL +SS ++P++V++ PTA
Sbjct: 268 AMAYRPVLEGKSCIIGDQSGSGKTLAYLCPVVQNLRKEEVEGLHRSSPRNPRVVVLTPTA 327

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISK GVPFRSMV TGGFRQ+TQLE+L + +DVLIATPGR + L+ EGF
Sbjct: 328 ELASQVLNNCRSISKSGVPFRSMVATGGFRQKTQLESLDQELDVLIATPGRFLYLLQEGF 387

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           +QL+NLRC V+DEVDIL+ +E FE  L  L+  AP+ TQYLFVTATLP+DIYN +VE FP
Sbjct: 388 VQLNNLRCVVLDEVDILYGEESFEQVLHQLITVAPLTTQYLFVTATLPLDIYNKVVETFP 447

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCE+IMGPGVHR S  LEE+LVDCSG+D + K P+ AFSNKK AL++I E +PV KTI+F
Sbjct: 448 DCELIMGPGVHRTSSRLEEILVDCSGDDNEEKNPETAFSNKKSALVKIIEESPVRKTIIF 507

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCR+VENAL+R D+K S ++V PFHAAL ++ R+AN++ F N  +   S FLVCT
Sbjct: 508 CNKIETCRKVENALRRVDRKASQIKVLPFHAALDQQQRIANIKEFLNKQTAD-SMFLVCT 567

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDF NV+HV+LFD+PRDPSEYVRRVGRTARGA+G GKAF+F VGKQVSLARR++
Sbjct: 568 DRASRGIDFANVNHVVLFDYPRDPSEYVRRVGRTARGASGNGKAFVFAVGKQVSLARRVM 627

Query: 362 ERNRKGHPLHDVP 375
           ERN KGHPLHDVP
Sbjct: 628 ERNIKGHPLHDVP 639

BLAST of Spg031480 vs. ExPASy Swiss-Prot
Match: Q56X76 (DEAD-box ATP-dependent RNA helicase 39 OS=Arabidopsis thaliana OX=3702 GN=RH39 PE=2 SV=2)

HSP 1 Score: 192.2 bits (487), Expect = 1.1e-47
Identity = 125/379 (32.98%), Postives = 201/379 (53.03%), Query Frame = 0

Query: 7   SVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTAELASQ 66
           +V+E KS ++   +GSGKTLAYL P++Q +R++E    +K+  + P+ V++ PT EL+ Q
Sbjct: 145 AVMERKSVVLGSHTGSGKTLAYLLPIVQLMREDEANLGKKTKPRRPRTVVLCPTRELSEQ 204

Query: 67  VLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGFLQLSN 126
           V +  +SIS     FRS++V+GG R R Q ++L   +D+++ TPGR++  I EG +   +
Sbjct: 205 VYRVAKSISHH-ARFRSILVSGGSRIRPQEDSLNNAIDMVVGTPGRILQHIEEGNMVYGD 264

Query: 127 LRCAVMDEVDILFNDEDFEGALRTLMKSAPVNT----------QYLFVTATLPVDIYNTL 186
           +   V+DE D +F D  F   +R  +  AP+N           Q + VTAT+ + +   +
Sbjct: 265 IAYLVLDEADTMF-DRGFGPEIRKFL--APLNQRALKTNDQGFQTVLVTATMTMAVQKLV 324

Query: 187 VENFPDCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPV- 246
            E F   E +    +H+         +  SG ++           K +ALLQ+ E +   
Sbjct: 325 DEEFQGIEHLRTSTLHKKIANARHDFIKLSGGED-----------KLEALLQVLEPSLAK 384

Query: 247 -SKTIVFCNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQV 306
            SK +VFCN + + R V++ L       + +    +H  +  E R+ N++ F +   +  
Sbjct: 385 GSKVMVFCNTLNSSRAVDHYLSE-----NQISTVNYHGEVPAEQRVENLKKFKDEEGD-- 444

Query: 307 SQFLVCTDRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQV 366
              LVCTD A+RG+D  +VDHV++FDFP++  +Y+ R GRTAR    KGK    V  K  
Sbjct: 445 CPTLVCTDLAARGLDL-DVDHVVMFDFPKNSIDYLHRTGRTAR-MGAKGKVTSLVSRKDQ 499

Query: 367 SLARRIVERNRKGHPLHDV 374
            LA RI E  R    L  +
Sbjct: 505 MLAARIEEAMRNNESLESL 499

BLAST of Spg031480 vs. ExPASy Swiss-Prot
Match: Q5VRY0 (DEAD-box ATP-dependent RNA helicase 39 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0184500 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.9e-45
Identity = 124/374 (33.16%), Postives = 190/374 (50.80%), Query Frame = 0

Query: 7   SVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTAELASQ 66
           +V+ G S ++   +GSGKTLAYL PL+Q LR++E         + P+ V++ PT EL  Q
Sbjct: 143 AVLAGTSVVLGSHTGSGKTLAYLLPLVQLLRRDEAMLGMSMKPRRPRAVVLCPTRELTEQ 202

Query: 67  VLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGFLQLSN 126
           V +  +SIS     FRS +V+GG R R Q ++L   VD+++ TPGR++  I +G +   +
Sbjct: 203 VFRVAKSISHH-ARFRSTMVSGGSRIRPQEDSLNMPVDMVVGTPGRILDHIKDGNMVYGD 262

Query: 127 LRCAVMDEVDILFNDEDFEGALRTLM--------KSAPVNTQYLFVTATLPVDIYNTLVE 186
           ++  V+DE D +F D+ F   +R  +        K      Q + VTAT+   +   + E
Sbjct: 263 IKYLVLDEADTMF-DQGFGPDIRKFLAPLKNRAAKPGDQGFQTVLVTATMTKAVQKLIDE 322

Query: 187 NFPDCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPV--S 246
            F     +      +         +  SG +           NK +ALLQ+ E +    +
Sbjct: 323 EFEGIVHLRTTTFQKRVATARHDFIKLSGSE-----------NKLEALLQVLEPSLAKGN 382

Query: 247 KTIVFCNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQ 306
           K +VFCN + + R V++ L       + +    +H  +  E R+ N+  F N   +    
Sbjct: 383 KVMVFCNTLNSSRAVDHFLTE-----NQISTVNYHGEVPAEERVENLNKFRNEEGD--CP 442

Query: 307 FLVCTDRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSL 366
            LVCTD A+RG+D  +VDHVI+FDFP +  +Y+ R GRTAR    KGK    V  K V+L
Sbjct: 443 TLVCTDLAARGLDL-DVDHVIMFDFPSNSIDYLHRTGRTAR-MGAKGKVTSLVAKKDVTL 494

Query: 367 ARRIVERNRKGHPL 371
           A RI E  +K   L
Sbjct: 503 ATRIEEAMKKNESL 494

BLAST of Spg031480 vs. ExPASy Swiss-Prot
Match: Q0UR48 (ATP-dependent RNA helicase HAS1 OS=Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) OX=321614 GN=HAS1 PE=3 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.0e-37
Identity = 113/363 (31.13%), Postives = 178/363 (49.04%), Query Frame = 0

Query: 3   KAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTAE 62
           KA   ++ GK  + + ++GSGKTLA+L P I     E L  +R        ++++ PT E
Sbjct: 160 KAIPPLLSGKDVLGAAKTGSGKTLAFLIPAI-----EMLSSMRFKPRNGTGVIVVSPTRE 219

Query: 63  LASQVLQNCRSI-SKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILIN--E 122
           LA Q+    R +  K    F   +V GG  +R + E L +GV++LIATPGRL+  ++  +
Sbjct: 220 LALQIFGVARELMEKHSQTFG--IVIGGANRRAEAEKLAKGVNLLIATPGRLLDHLHNTQ 279

Query: 123 GFLQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVEN 182
           GF+   NL+  ++DE D +  +  FE  +R+++K  P + Q +  +AT       T VE+
Sbjct: 280 GFV-FKNLKSLIIDEADRIL-EVGFEDEMRSIIKILPTDRQTMLFSAT-----QTTKVED 339

Query: 183 FPDCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTI 242
                +  GP    +    E   V+   +       D  F      L    +     K I
Sbjct: 340 LARISLKAGPLYINVDYRKEHSTVEGLEQGYVICDSDTRFR----LLFSFLKKHQKKKVI 399

Query: 243 VFCNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLV 302
           VF +   + +     L   D     L V   H  L +++R      F N+ S      L+
Sbjct: 400 VFFSSCNSVKFYAELLNYID-----LPVLELHGKLKQQARTNRFFEFCNAQSGT----LI 459

Query: 303 CTDRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARR 362
           CTD A+RG+D P VD VI FD P DP +Y+ RVGRTARG+ GKG++ +F++  ++   + 
Sbjct: 460 CTDVAARGLDIPEVDWVIQFDPPDDPRDYIHRVGRTARGSEGKGRSLMFLLPSEIGFLKL 495

BLAST of Spg031480 vs. ExPASy TrEMBL
Match: A0A5A7UF84 (DEAD-box ATP-dependent RNA helicase 50 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1769G00100 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.2e-205
Identity = 363/380 (95.53%), Postives = 372/380 (97.89%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFASVIEGKSCIISDQSGSGKTLAYL PLIQRLRQEELEG+RKSSS+SPQIVIIVPTA
Sbjct: 269 AKAFASVIEGKSCIISDQSGSGKTLAYLVPLIQRLRQEELEGVRKSSSKSPQIVIIVPTA 328

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQL+NLQEGVDVLIATPGRLM+LINEGF
Sbjct: 329 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLDNLQEGVDVLIATPGRLMLLINEGF 388

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           L LSNLRCAVMDEVDILFNDEDFE ALR+LMKSAPVNTQYLFVTATLPVDIYNTLVENFP
Sbjct: 389 LLLSNLRCAVMDEVDILFNDEDFEVALRSLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 448

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSGEDEQNKTPDAAF NKKDALLQIAEGTPVSKTIVF
Sbjct: 449 DCEVIMGPGVHRISPSLEEVLVDCSGEDEQNKTPDAAFLNKKDALLQIAEGTPVSKTIVF 508

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCR+VENALQRFDKKGS LQVFPFHAALARESRLANMEAFTNSHSNQVS+FLVCT
Sbjct: 509 CNKIETCRKVENALQRFDKKGSRLQVFPFHAALARESRLANMEAFTNSHSNQVSKFLVCT 568

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQV LARRI+
Sbjct: 569 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVPLARRII 628

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 629 ERNRKGHPLHDVPSAYELTY 648

BLAST of Spg031480 vs. ExPASy TrEMBL
Match: A0A1S3AXT4 (DEAD-box ATP-dependent RNA helicase 50 OS=Cucumis melo OX=3656 GN=LOC103483937 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.2e-205
Identity = 363/380 (95.53%), Postives = 372/380 (97.89%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFASVIEGKSCIISDQSGSGKTLAYL PLIQRLRQEELEG+RKSSS+SPQIVIIVPTA
Sbjct: 269 AKAFASVIEGKSCIISDQSGSGKTLAYLVPLIQRLRQEELEGVRKSSSKSPQIVIIVPTA 328

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQL+NLQEGVDVLIATPGRLM+LINEGF
Sbjct: 329 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLDNLQEGVDVLIATPGRLMLLINEGF 388

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           L LSNLRCAVMDEVDILFNDEDFE ALR+LMKSAPVNTQYLFVTATLPVDIYNTLVENFP
Sbjct: 389 LLLSNLRCAVMDEVDILFNDEDFEVALRSLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 448

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSGEDEQNKTPDAAF NKKDALLQIAEGTPVSKTIVF
Sbjct: 449 DCEVIMGPGVHRISPSLEEVLVDCSGEDEQNKTPDAAFLNKKDALLQIAEGTPVSKTIVF 508

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCR+VENALQRFDKKGS LQVFPFHAALARESRLANMEAFTNSHSNQVS+FLVCT
Sbjct: 509 CNKIETCRKVENALQRFDKKGSRLQVFPFHAALARESRLANMEAFTNSHSNQVSKFLVCT 568

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQV LARRI+
Sbjct: 569 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVPLARRII 628

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 629 ERNRKGHPLHDVPSAYELTY 648

BLAST of Spg031480 vs. ExPASy TrEMBL
Match: A0A6J1IRK9 (DEAD-box ATP-dependent RNA helicase 50 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479352 PE=4 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.1e-204
Identity = 362/380 (95.26%), Postives = 371/380 (97.63%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEEL+GL+K SSQSPQIVIIVPTA
Sbjct: 270 AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELQGLQKPSSQSPQIVIIVPTA 329

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQL+NLQEGVDVLIATPGRLM+LINEGF
Sbjct: 330 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLDNLQEGVDVLIATPGRLMLLINEGF 389

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           LQLSNLRCAVMDEVDILFNDEDFE AL+TLMKSAPVN QYLFVTATLPVDIYNTLVE FP
Sbjct: 390 LQLSNLRCAVMDEVDILFNDEDFEVALQTLMKSAPVNAQYLFVTATLPVDIYNTLVEKFP 449

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSGEDEQNKTPDAAF NKKDALLQIAEGTPV KTIVF
Sbjct: 450 DCEVIMGPGVHRISPSLEEVLVDCSGEDEQNKTPDAAFLNKKDALLQIAEGTPVLKTIVF 509

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCRRVENALQRFDKKGS LQVFPFHAALAR+SRLANMEAFTNSHSNQVS+FLVCT
Sbjct: 510 CNKIETCRRVENALQRFDKKGSRLQVFPFHAALARDSRLANMEAFTNSHSNQVSKFLVCT 569

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRI+
Sbjct: 570 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRII 629

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 630 ERNRKGHPLHDVPSAYELTY 649

BLAST of Spg031480 vs. ExPASy TrEMBL
Match: A0A6J1IX45 (DEAD-box ATP-dependent RNA helicase 50 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479352 PE=4 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.1e-204
Identity = 362/380 (95.26%), Postives = 371/380 (97.63%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEEL+GL+K SSQSPQIVIIVPTA
Sbjct: 265 AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELQGLQKPSSQSPQIVIIVPTA 324

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQL+NLQEGVDVLIATPGRLM+LINEGF
Sbjct: 325 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLDNLQEGVDVLIATPGRLMLLINEGF 384

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           LQLSNLRCAVMDEVDILFNDEDFE AL+TLMKSAPVN QYLFVTATLPVDIYNTLVE FP
Sbjct: 385 LQLSNLRCAVMDEVDILFNDEDFEVALQTLMKSAPVNAQYLFVTATLPVDIYNTLVEKFP 444

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSGEDEQNKTPDAAF NKKDALLQIAEGTPV KTIVF
Sbjct: 445 DCEVIMGPGVHRISPSLEEVLVDCSGEDEQNKTPDAAFLNKKDALLQIAEGTPVLKTIVF 504

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCRRVENALQRFDKKGS LQVFPFHAALAR+SRLANMEAFTNSHSNQVS+FLVCT
Sbjct: 505 CNKIETCRRVENALQRFDKKGSRLQVFPFHAALARDSRLANMEAFTNSHSNQVSKFLVCT 564

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRI+
Sbjct: 565 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRII 624

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 625 ERNRKGHPLHDVPSAYELTY 644

BLAST of Spg031480 vs. ExPASy TrEMBL
Match: A0A6J1C9F7 (DEAD-box ATP-dependent RNA helicase 50 OS=Momordica charantia OX=3673 GN=LOC111009193 PE=4 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 3.2e-204
Identity = 362/380 (95.26%), Postives = 371/380 (97.63%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           AKAFA VIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQ+PQIVIIVPTA
Sbjct: 259 AKAFAPVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQNPQIVIIVPTA 318

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISKFGVPFRSMVVTGGFRQ+TQLENLQEGVDVLIATPGRLM+LINEGF
Sbjct: 319 ELASQVLSNCRSISKFGVPFRSMVVTGGFRQKTQLENLQEGVDVLIATPGRLMLLINEGF 378

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           L+LSNLRCAVMDEVDILFNDEDFE AL+TLMKSAPVNTQYLFVTATLPV+IYNTLVENFP
Sbjct: 379 LKLSNLRCAVMDEVDILFNDEDFEVALQTLMKSAPVNTQYLFVTATLPVEIYNTLVENFP 438

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEVIMGPGVHRISP LEEVLVDCSG DEQNKTPDAAF NKKDALLQIAE TPV +TIVF
Sbjct: 439 DCEVIMGPGVHRISPCLEEVLVDCSGVDEQNKTPDAAFLNKKDALLQIAEQTPVLRTIVF 498

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNS SNQVSQFLVCT
Sbjct: 499 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSQSNQVSQFLVCT 558

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQV+LARRI+
Sbjct: 559 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVTLARRII 618

Query: 362 ERNRKGHPLHDVPSAYELTY 382
           ERNRKGHPLHDVPSAYELTY
Sbjct: 619 ERNRKGHPLHDVPSAYELTY 638

BLAST of Spg031480 vs. TAIR 10
Match: AT3G06980.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 579.3 bits (1492), Expect = 2.2e-165
Identity = 282/379 (74.41%), Postives = 322/379 (84.96%), Query Frame = 0

Query: 2   AKAFASVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTA 61
           A AF+ VI+GKSCII+DQSGSGKTLAYL P+IQRLR+EEL+G  KSS   P+++++VPTA
Sbjct: 402 AMAFSPVIDGKSCIIADQSGSGKTLAYLVPVIQRLREEELQGHSKSSPGCPRVIVLVPTA 461

Query: 62  ELASQVLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGF 121
           ELASQVL NCRSISK GVPFRSMVVTGGFRQRTQLENL++GVDVLIATPGR   L+NEG 
Sbjct: 462 ELASQVLANCRSISKSGVPFRSMVVTGGFRQRTQLENLEQGVDVLIATPGRFTYLMNEGI 521

Query: 122 LQLSNLRCAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYNTLVENFP 181
           L LSNLRCA++DEVDILF D++FE AL+ L+ S+PV  QYLFVTATLP++IYN LVE FP
Sbjct: 522 LGLSNLRCAILDEVDILFGDDEFEAALQNLINSSPVTAQYLFVTATLPLEIYNKLVEVFP 581

Query: 182 DCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIVF 241
           DCEV+MGP VHR+S  LEE LVDCSG+D   KTP+ AF NKK ALLQI E  PVSKTI+F
Sbjct: 582 DCEVVMGPRVHRVSNALEEFLVDCSGDDNAEKTPETAFQNKKTALLQIMEENPVSKTIIF 641

Query: 242 CNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVCT 301
           CNKIETCR+VEN  +R D+K   L V PFHAAL++ESRL NM+ FT+S   + S FLVCT
Sbjct: 642 CNKIETCRKVENIFKRVDRKERQLHVLPFHAALSQESRLTNMQEFTSSQPEENSLFLVCT 701

Query: 302 DRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQVSLARRIV 361
           DRASRGIDF  VDHV+LFDFPRDPSEYVRRVGRTARGA GKGKAFIFVVGKQV LARRI+
Sbjct: 702 DRASRGIDFSGVDHVVLFDFPRDPSEYVRRVGRTARGARGKGKAFIFVVGKQVGLARRII 761

Query: 362 ERNRKGHPLHDVPSAYELT 381
           ERN KGHP+HDVP+AYE T
Sbjct: 762 ERNEKGHPVHDVPNAYEFT 780

BLAST of Spg031480 vs. TAIR 10
Match: AT4G09730.1 (RH39 )

HSP 1 Score: 192.2 bits (487), Expect = 7.7e-49
Identity = 125/379 (32.98%), Postives = 201/379 (53.03%), Query Frame = 0

Query: 7   SVIEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTAELASQ 66
           +V+E KS ++   +GSGKTLAYL P++Q +R++E    +K+  + P+ V++ PT EL+ Q
Sbjct: 145 AVMERKSVVLGSHTGSGKTLAYLLPIVQLMREDEANLGKKTKPRRPRTVVLCPTRELSEQ 204

Query: 67  VLQNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGFLQLSN 126
           V +  +SIS     FRS++V+GG R R Q ++L   +D+++ TPGR++  I EG +   +
Sbjct: 205 VYRVAKSISHH-ARFRSILVSGGSRIRPQEDSLNNAIDMVVGTPGRILQHIEEGNMVYGD 264

Query: 127 LRCAVMDEVDILFNDEDFEGALRTLMKSAPVNT----------QYLFVTATLPVDIYNTL 186
           +   V+DE D +F D  F   +R  +  AP+N           Q + VTAT+ + +   +
Sbjct: 265 IAYLVLDEADTMF-DRGFGPEIRKFL--APLNQRALKTNDQGFQTVLVTATMTMAVQKLV 324

Query: 187 VENFPDCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPV- 246
            E F   E +    +H+         +  SG ++           K +ALLQ+ E +   
Sbjct: 325 DEEFQGIEHLRTSTLHKKIANARHDFIKLSGGED-----------KLEALLQVLEPSLAK 384

Query: 247 -SKTIVFCNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQV 306
            SK +VFCN + + R V++ L       + +    +H  +  E R+ N++ F +   +  
Sbjct: 385 GSKVMVFCNTLNSSRAVDHYLSE-----NQISTVNYHGEVPAEQRVENLKKFKDEEGD-- 444

Query: 307 SQFLVCTDRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTARGATGKGKAFIFVVGKQV 366
              LVCTD A+RG+D  +VDHV++FDFP++  +Y+ R GRTAR    KGK    V  K  
Sbjct: 445 CPTLVCTDLAARGLDL-DVDHVVMFDFPKNSIDYLHRTGRTAR-MGAKGKVTSLVSRKDQ 499

Query: 367 SLARRIVERNRKGHPLHDV 374
            LA RI E  R    L  +
Sbjct: 505 MLAARIEEAMRNNESLESL 499

BLAST of Spg031480 vs. TAIR 10
Match: AT3G61240.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 136.3 bits (342), Expect = 5.0e-32
Identity = 102/337 (30.27%), Postives = 159/337 (47.18%), Query Frame = 0

Query: 9   IEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTAELASQVL 68
           + G   +   ++G+GKT A+  P+++++           ++   Q +I+VPT ELA Q  
Sbjct: 159 LTGSDILARAKNGTGKTGAFCIPVLEKI---------DPNNNVIQAMILVPTRELALQTS 218

Query: 69  QNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGFLQLSNLR 128
           Q C+ +SK+ +  + MV TGG   R  +  L + V +L+ TPGR++ L  +G   L +  
Sbjct: 219 QVCKELSKY-LNIQVMVTTGGTSLRDDIMRLHQPVHLLVGTPGRILDLTKKGVCVLKDCA 278

Query: 129 CAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDI--------YNTLVENF 188
             VMDE D L + E F+ +L  L++  P N Q+L  +AT PV +            V N 
Sbjct: 279 MLVMDEADKLLSAE-FQPSLEELIQFLPQNRQFLMFSATFPVTVKAFKDRHLRKPYVINL 338

Query: 189 PDCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIV 248
            D   +M  GV +   F+EE                     K   L  +     ++++I+
Sbjct: 339 MDQLTLM--GVTQYYAFVEE-------------------RQKVHCLNTLFSKLQINQSII 398

Query: 249 FCNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVC 308
           FCN +    RVE   ++  + G     F  HA + ++ R      F N       + LVC
Sbjct: 399 FCNSV---NRVELLAKKITELG--YSCFYIHAKMVQDHRNRVFHEFRNG----ACRNLVC 454

Query: 309 TDRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTAR 338
           TD  +RGID   V+ VI FDFPR    Y+ RVGR+ R
Sbjct: 459 TDLFTRGIDIQAVNVVINFDFPRTSESYLHRVGRSGR 454

BLAST of Spg031480 vs. TAIR 10
Match: AT3G61240.2 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 136.3 bits (342), Expect = 5.0e-32
Identity = 102/337 (30.27%), Postives = 159/337 (47.18%), Query Frame = 0

Query: 9   IEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTAELASQVL 68
           + G   +   ++G+GKT A+  P+++++           ++   Q +I+VPT ELA Q  
Sbjct: 159 LTGSDILARAKNGTGKTGAFCIPVLEKI---------DPNNNVIQAMILVPTRELALQTS 218

Query: 69  QNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGFLQLSNLR 128
           Q C+ +SK+ +  + MV TGG   R  +  L + V +L+ TPGR++ L  +G   L +  
Sbjct: 219 QVCKELSKY-LNIQVMVTTGGTSLRDDIMRLHQPVHLLVGTPGRILDLTKKGVCVLKDCA 278

Query: 129 CAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDI--------YNTLVENF 188
             VMDE D L + E F+ +L  L++  P N Q+L  +AT PV +            V N 
Sbjct: 279 MLVMDEADKLLSAE-FQPSLEELIQFLPQNRQFLMFSATFPVTVKAFKDRHLRKPYVINL 338

Query: 189 PDCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIV 248
            D   +M  GV +   F+EE                     K   L  +     ++++I+
Sbjct: 339 MDQLTLM--GVTQYYAFVEE-------------------RQKVHCLNTLFSKLQINQSII 398

Query: 249 FCNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVC 308
           FCN +    RVE   ++  + G     F  HA + ++ R      F N       + LVC
Sbjct: 399 FCNSV---NRVELLAKKITELG--YSCFYIHAKMVQDHRNRVFHEFRNG----ACRNLVC 454

Query: 309 TDRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTAR 338
           TD  +RGID   V+ VI FDFPR    Y+ RVGR+ R
Sbjct: 459 TDLFTRGIDIQAVNVVINFDFPRTSESYLHRVGRSGR 454

BLAST of Spg031480 vs. TAIR 10
Match: AT2G45810.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 132.1 bits (331), Expect = 9.4e-31
Identity = 101/337 (29.97%), Postives = 157/337 (46.59%), Query Frame = 0

Query: 9   IEGKSCIISDQSGSGKTLAYLAPLIQRLRQEELEGLRKSSSQSPQIVIIVPTAELASQVL 68
           + G   +   ++G+GKT A+  P ++++  E         +   Q VI+VPT ELA Q  
Sbjct: 189 LTGSDILARAKNGTGKTGAFCIPTLEKIDPE---------NNVIQAVILVPTRELALQTS 248

Query: 69  QNCRSISKFGVPFRSMVVTGGFRQRTQLENLQEGVDVLIATPGRLMILINEGFLQLSNLR 128
           Q C+ +SK+ +    MV TGG   R  +  L + V +L+ TPGR++ L  +G   L +  
Sbjct: 249 QVCKELSKY-LKIEVMVTTGGTSLRDDIMRLYQPVHLLVGTPGRILDLAKKGVCVLKDCA 308

Query: 129 CAVMDEVDILFNDEDFEGALRTLMKSAPVNTQYLFVTATLPVDIYN--------TLVENF 188
             VMDE D L + E F+ ++  L++  P + Q L  +AT PV + +          + N 
Sbjct: 309 MLVMDEADKLLSVE-FQPSIEELIQFLPESRQILMFSATFPVTVKSFKDRYLKKPYIINL 368

Query: 189 PDCEVIMGPGVHRISPFLEEVLVDCSGEDEQNKTPDAAFSNKKDALLQIAEGTPVSKTIV 248
            D   +M  GV +   F+EE                     K   L  +     ++++I+
Sbjct: 369 MDQLTLM--GVTQYYAFVEE-------------------RQKVHCLNTLFSKLQINQSII 428

Query: 249 FCNKIETCRRVENALQRFDKKGSHLQVFPFHAALARESRLANMEAFTNSHSNQVSQFLVC 308
           FCN +    RVE   ++  + G     F  HA + ++ R      F N       + LVC
Sbjct: 429 FCNSV---NRVELLAKKITELG--YSCFYIHAKMVQDHRNRVFHDFRNG----ACRNLVC 484

Query: 309 TDRASRGIDFPNVDHVILFDFPRDPSEYVRRVGRTAR 338
           TD  +RGID   V+ VI FDFPR    Y+ RVGR+ R
Sbjct: 489 TDLFTRGIDIQAVNVVINFDFPRTSESYLHRVGRSGR 484

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008439020.14.6e-20595.53PREDICTED: DEAD-box ATP-dependent RNA helicase 50 [Cucumis melo] >KAA0052139.1 D... [more]
XP_022979728.12.3e-20495.26DEAD-box ATP-dependent RNA helicase 50 isoform X2 [Cucurbita maxima][more]
XP_022979726.12.3e-20495.26DEAD-box ATP-dependent RNA helicase 50 isoform X1 [Cucurbita maxima] >XP_0229797... [more]
XP_022137867.16.6e-20495.26DEAD-box ATP-dependent RNA helicase 50 [Momordica charantia][more]
XP_023528025.18.7e-20495.00DEAD-box ATP-dependent RNA helicase 50 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8GUG73.1e-16474.41DEAD-box ATP-dependent RNA helicase 50 OS=Arabidopsis thaliana OX=3702 GN=RH50 P... [more]
Q0DVX22.9e-15469.71DEAD-box ATP-dependent RNA helicase 50 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q56X761.1e-4732.98DEAD-box ATP-dependent RNA helicase 39 OS=Arabidopsis thaliana OX=3702 GN=RH39 P... [more]
Q5VRY02.9e-4533.16DEAD-box ATP-dependent RNA helicase 39 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q0UR481.0e-3731.13ATP-dependent RNA helicase HAS1 OS=Phaeosphaeria nodorum (strain SN15 / ATCC MYA... [more]
Match NameE-valueIdentityDescription
A0A5A7UF842.2e-20595.53DEAD-box ATP-dependent RNA helicase 50 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3AXT42.2e-20595.53DEAD-box ATP-dependent RNA helicase 50 OS=Cucumis melo OX=3656 GN=LOC103483937 P... [more]
A0A6J1IRK91.1e-20495.26DEAD-box ATP-dependent RNA helicase 50 isoform X1 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1IX451.1e-20495.26DEAD-box ATP-dependent RNA helicase 50 isoform X2 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1C9F73.2e-20495.26DEAD-box ATP-dependent RNA helicase 50 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
Match NameE-valueIdentityDescription
AT3G06980.12.2e-16574.41DEA(D/H)-box RNA helicase family protein [more]
AT4G09730.17.7e-4932.98RH39 [more]
AT3G61240.15.0e-3230.27DEA(D/H)-box RNA helicase family protein [more]
AT3G61240.25.0e-3230.27DEA(D/H)-box RNA helicase family protein [more]
AT2G45810.19.4e-3129.97DEA(D/H)-box RNA helicase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 249..339
e-value: 2.3E-20
score: 83.6
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 222..338
e-value: 4.5E-24
score: 84.9
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 221..381
score: 17.903339
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 2..201
e-value: 4.2E-31
score: 119.3
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 5..186
score: 25.05184
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 2..189
e-value: 2.8E-45
score: 156.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 198..372
e-value: 1.2E-36
score: 127.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 51..360
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 2..172
e-value: 7.4E-35
score: 120.3
NoneNo IPR availablePANTHERPTHR47960:SF1DEAD-BOX ATP-DEPENDENT RNA HELICASE 50coord: 2..379
NoneNo IPR availablePANTHERPTHR47960DEAD-BOX ATP-DEPENDENT RNA HELICASE 50coord: 2..379
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 221..349
e-value: 1.036E-37
score: 130.705
NoneNo IPR availableCDDcd00268DEADccoord: 2..186
e-value: 4.09778E-59
score: 188.42
IPR014013Helicase superfamily 1/2, ATP-binding domain, DinG/Rad3-typePROSITEPS51193HELICASE_ATP_BIND_2coord: 1..290
score: 8.583851

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031480.1Spg031480.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006968 cellular defense response
biological_process GO:0071369 cellular response to ethylene stimulus
biological_process GO:0071395 cellular response to jasmonic acid stimulus
biological_process GO:0071446 cellular response to salicylic acid stimulus
biological_process GO:0050832 defense response to fungus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003724 RNA helicase activity