Spg031462 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031462
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionProtein of unknown function (DUF668)
Locationscaffold11: 40185600 .. 40188358 (-)
RNA-Seq ExpressionSpg031462
SyntenySpg031462
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCACACTTCTTGAACCAATTCCTCCCTTCAATCTCCATGATTTCAGAATTTCCTATCCAACTGCCATGGATTCATTCCTTTGAGAGCTCTCAAAATGGGGAACAGCCCAAGTTCCAATCTTCCCCACAAGCCACCTCCAAATCGCACCGCCACAATCGGCATTCTTTCCTTCGAAATCGCCAATGTAATTTCCCGAGCCATCCACCTCCACAACACCCTCAAACACCTCCAATTCTCCAATCTCCGCACCCAAATTCTCACCGCCGAGGGAATTACAACCCTCATCTCCTCCGACGAGAATCGCCTCCTCCGACTGGTCCTGGCCGAGAAAGTCCATGACTTGAACCAGGTGGCCGGCTCTGTCTCCAGACTCGGGAAGAGATGCTCTGTTCCGGCCCTGCAGGGGTTCGAGCATATATACGCCGACGTCGTCAGTGGGAATCTCGAGGGGAAGAAGCTTGGGATTTTGACTAAGGATATGGATGGAATTGTCAAGAAAATGGAAAGCTACGTGAAACGGACGGCGAATTTGTACAGTGCGATCGAGGTCTCGAATGGTCATAAATGGCAGACAAAGAACAATATCAAGCATCTCAAACACATCTCACTTTGGAACAAAAGCTACAACAAAGTGGTTCAACTTTTGGGTAGAAATCATAAAAACTTAACTCAACCTTATAGTATAGGTTGCCCTTTTTCTTCATCTTTTTATTCTTATTCTTTTTAACTCTCATCAGCTTGTGGATTGTGAATTAGCACAAATTCCAATTCGTGTCGAACAAACTTTTGAAAAATTATTGGATGTGACGACGTTTCCGTTTAAAATATGTATGCACACACTAAATAATATTCACCATGAATATATCTTCCAACGGTCTACCATTAAATATTTGAAGCTAGTTACATTTTTTTAAATTTTAAAAGAAAAAATCTTCTTCCTATTAACTAGTTAATATATATTTCGTACTCTCAAATATTTATATAAAATTATTTGCGCCAAGATTATTGTTTCACCCAATCACTTGAATATATATAATATAATGTTAAATCACCATTAAACTAAAAAAAACATGGAAGGGGTTTGAGGTTTGACTTCAGCATCTTAAACCTCAATCTAGTTTTAAGAAATTTCATATTAGAATGCATGCTCAAAAAATCTAGGTATAAAAAACCTATATCTCGAGTTTAGATCTAGTAAGTCTAGATTTAAGAAAATAGTATCAAAAAATGATTTTGATGTAAGGGACAATAGACACGATATTTGTCCACTTTGGACATAAGCTCTTGTGGCTTTGCTTTTGTTCACCCCAAAAGGCCTCATTAAGATACTTGTCCTCCCTTATAAGTCCATGATCCTCCTATTATCTAGTCAATGTGGGACTTTGGTCGCACTCCCTACAATCTTTTCCTCGAACAAAATACCACCGAGTCTCTCCTCAAATAGTCTTCTATCCGTCAAACTCAGAACTCTCCTCTACCAAGAGTCCAGCCGTTGTCCCTTACAATTGAATTCTATGAATTTGTCTCAACCCTAAACTTCACAAACTCATAAGACAACACACAAGTTCAACATCTTAGGTTAAACCATCCAAATCTTGACCCCTCTTGTAAGTTTTATAAATGTTTCTATATTTAATAATTTATTTTGACAGGTAAAATGGTAGTGTTGCTATACGCAAGAATCAGTCTAGTATTCCACGACCACCTTGCCGGAACGGACGTCAAATCATCCATCCACGCCGACTCCTCCACCGTCGGCGGCGCGGCTCTAACCGTCCGTTACGCCAACATCATAATCATAACAGAGAAATTCCTCCGCCAACCCCATCTCGTCGGCAACGACGCCAGAGATGACTTGTACGAGATGCTAACCACAAGCTTAAAGTCATCTCTAAGAACCCACTTGAAATGCAACGCCAGAGCCCAATCTATCTACGACGCCACCGTCTGCAGGTACTGGAAACAGGCCGCCGATCGGATTCTGGAGTGGCTGGCGCCGGTGGCCCACGACACGATCCGGTGGCAGAACGAGCGTGGGTTCGAACAGTATCGTCAAATTGGGAAGATATCGACGAACGTTGCGCTGGTTCAGACGCTGTACTTTGCTGACCGTGAGAAGACTGAAGCGTCCATTTGCAAGGTTCTTGTCGCTTTGAATTATCTTTGTCGGTATGAGAATCAACAGGTTGCGTTGTTGGATTGTGTTAGTAGTAGCTTTGATATTGAGGATCGAGTGGATTGGGAATTGCACGTCAAAGGTTCTTGATTTTCCTTAGTGGGCTTCCATGTATAGAGAAAACCTTGGAGAGGTTTTAGGTAAATTATTAGGTATGAGACTCACTCTTTAAAACCGTGGAATGTACATCTTCAAAGTTGTTTGTCCCTAATAACTGTTGGATACAAAATGAAGGAACTTTAACATTTCTCTCTTATGTCTAACTGTGCAAGACTACCTACTTCATTATACTAAGATCACCTAATAATTTTAGAAGAGAAATTATGGGATGAGAATGGTAATTTTGCATAGTTGGATATAAGAGAGAAATTGTAATAGTACCTCTCGATTTTGTATTGAACGTTATTAGGGGACAAACAAATTTAAAGATCTGCATCCGATGATTTTAAAGAGACAACCGCATTGTGACGGTCTTATCGAATAATTTTTAGTTTTAGAGTTTCTTATTGCATGAATTTTTTTTGTTTGTTTTTTTTTTAATTTTATTTTTTAGAAAAGGAATACTTTATTGAGAAACGTAT

mRNA sequence

ATGGGGAACAGCCCAAGTTCCAATCTTCCCCACAAGCCACCTCCAAATCGCACCGCCACAATCGGCATTCTTTCCTTCGAAATCGCCAATGTAATTTCCCGAGCCATCCACCTCCACAACACCCTCAAACACCTCCAATTCTCCAATCTCCGCACCCAAATTCTCACCGCCGAGGGAATTACAACCCTCATCTCCTCCGACGAGAATCGCCTCCTCCGACTGGTCCTGGCCGAGAAAGTCCATGACTTGAACCAGGTGGCCGGCTCTGTCTCCAGACTCGGGAAGAGATGCTCTGTTCCGGCCCTGCAGGGGTTCGAGCATATATACGCCGACGTCGTCAGTGGGAATCTCGAGGGGAAGAAGCTTGGGATTTTGACTAAGGATATGGATGGAATTGTCAAGAAAATGGAAAGCTACGTGAAACGGACGGCGAATTTGTACAGTGCGATCGAGGTCTCGAATGGTCATAAATGGCAGACAAAGAACAATATCAAGCATCTCAAACACATCTCACTTTGGAACAAAAGCTACAACAAAGTGGTTCAACTTTTGGGTAAAATGGTAGTGTTGCTATACGCAAGAATCAGTCTAGTATTCCACGACCACCTTGCCGGAACGGACGTCAAATCATCCATCCACGCCGACTCCTCCACCGTCGGCGGCGCGGCTCTAACCGTCCGTTACGCCAACATCATAATCATAACAGAGAAATTCCTCCGCCAACCCCATCTCGTCGGCAACGACGCCAGAGATGACTTGTACGAGATGCTAACCACAAGCTTAAAGTCATCTCTAAGAACCCACTTGAAATGCAACGCCAGAGCCCAATCTATCTACGACGCCACCGTCTGCAGGTACTGGAAACAGGCCGCCGATCGGATTCTGGAGTGGCTGGCGCCGGTGGCCCACGACACGATCCGGTGGCAGAACGAGCGTGGGTTCGAACAGTATCGTCAAATTGGGAAGATATCGACGAACGTTGCGCTGGTTCAGACGCTGTACTTTGCTGACCGTGAGAAGACTGAAGCGTCCATTTGCAAGGTTCTTGTCGCTTTGAATTATCTTTGTCGGTATGAGAATCAACAGGTTGCGTTGTTGGATTGTGTTAGTAGTAGCTTTGATATTGAGGATCGAGTGGATTGGGAATTGCACGTCAAAGGTTCTTGA

Coding sequence (CDS)

ATGGGGAACAGCCCAAGTTCCAATCTTCCCCACAAGCCACCTCCAAATCGCACCGCCACAATCGGCATTCTTTCCTTCGAAATCGCCAATGTAATTTCCCGAGCCATCCACCTCCACAACACCCTCAAACACCTCCAATTCTCCAATCTCCGCACCCAAATTCTCACCGCCGAGGGAATTACAACCCTCATCTCCTCCGACGAGAATCGCCTCCTCCGACTGGTCCTGGCCGAGAAAGTCCATGACTTGAACCAGGTGGCCGGCTCTGTCTCCAGACTCGGGAAGAGATGCTCTGTTCCGGCCCTGCAGGGGTTCGAGCATATATACGCCGACGTCGTCAGTGGGAATCTCGAGGGGAAGAAGCTTGGGATTTTGACTAAGGATATGGATGGAATTGTCAAGAAAATGGAAAGCTACGTGAAACGGACGGCGAATTTGTACAGTGCGATCGAGGTCTCGAATGGTCATAAATGGCAGACAAAGAACAATATCAAGCATCTCAAACACATCTCACTTTGGAACAAAAGCTACAACAAAGTGGTTCAACTTTTGGGTAAAATGGTAGTGTTGCTATACGCAAGAATCAGTCTAGTATTCCACGACCACCTTGCCGGAACGGACGTCAAATCATCCATCCACGCCGACTCCTCCACCGTCGGCGGCGCGGCTCTAACCGTCCGTTACGCCAACATCATAATCATAACAGAGAAATTCCTCCGCCAACCCCATCTCGTCGGCAACGACGCCAGAGATGACTTGTACGAGATGCTAACCACAAGCTTAAAGTCATCTCTAAGAACCCACTTGAAATGCAACGCCAGAGCCCAATCTATCTACGACGCCACCGTCTGCAGGTACTGGAAACAGGCCGCCGATCGGATTCTGGAGTGGCTGGCGCCGGTGGCCCACGACACGATCCGGTGGCAGAACGAGCGTGGGTTCGAACAGTATCGTCAAATTGGGAAGATATCGACGAACGTTGCGCTGGTTCAGACGCTGTACTTTGCTGACCGTGAGAAGACTGAAGCGTCCATTTGCAAGGTTCTTGTCGCTTTGAATTATCTTTGTCGGTATGAGAATCAACAGGTTGCGTTGTTGGATTGTGTTAGTAGTAGCTTTGATATTGAGGATCGAGTGGATTGGGAATTGCACGTCAAAGGTTCTTGA

Protein sequence

MGNSPSSNLPHKPPPNRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTLISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLGILTKDMDGIVKKMESYVKRTANLYSAIEVSNGHKWQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVFHDHLAGTDVKSSIHADSSTVGGAALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNARAQSIYDATVCRYWKQAADRILEWLAPVAHDTIRWQNERGFEQYRQIGKISTNVALVQTLYFADREKTEASICKVLVALNYLCRYENQQVALLDCVSSSFDIEDRVDWELHVKGS
Homology
BLAST of Spg031462 vs. NCBI nr
Match: KAG2306699.1 (hypothetical protein Bca52824_026447 [Brassica carinata])

HSP 1 Score: 372.5 bits (955), Expect = 4.5e-99
Identity = 208/468 (44.44%), Postives = 288/468 (61.54%), Query Frame = 0

Query: 6   SSNLPH---------KPPPNRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILT 65
           S+NL H         KP P +  TIGILSFE+AN++S+ +HLH +L   + S L++++  
Sbjct: 11  STNLKHALLLESSSKKPSPKQ--TIGILSFEVANIMSKTVHLHRSLSDAELSKLKSEVFL 70

Query: 66  AEGITTLISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGN 125
           ++G+T L+SSDEN LL L ++EK+ DL++VA  VSRLGK+C+ PALQGFEH+Y D+V+G 
Sbjct: 71  SQGVTKLVSSDENHLLDLAVSEKLDDLSRVASVVSRLGKKCNEPALQGFEHVYEDIVNGV 130

Query: 126 LEGKKLGILTKDMDGIVKKMESYVKRTANLYSAIEVSN-------------GHK------ 185
           ++ +KLG L KDM+ +VKKME +V  T NLY  +EV N              H+      
Sbjct: 131 IDFRKLGFLVKDMESMVKKMERFVNATCNLYCEMEVMNELEQAVVKLQRSGQHQGSVKAF 190

Query: 186 -----WQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVFHD-HLAGTD---- 245
                WQ + ++K L+  SLWN++Y+KVV++L + V  +Y RI  VF    L GT+    
Sbjct: 191 EQKLMWQ-RQDVKSLRDASLWNQTYDKVVEMLARTVCTIYGRIETVFGGLGLRGTEEFTR 250

Query: 246 ---------------------VKSSI--------------------------------HA 305
                                + +S+                                HA
Sbjct: 251 AADFGFPCGTNPGRMFMECLPMNNSVSRSYDDEEEDARITFPRTTRSSKFGSKSRLTQHA 310

Query: 306 DSSTVGGAALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNAR 365
            +ST+GG+AL++ YAN++I+ EK L+ PHL+G +ARDDLY+ML TSLK+SL+ +L+   +
Sbjct: 311 SASTIGGSALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSLKTSLKANLRSYLK 370

Query: 366 AQSIYDATVCRYWKQAADRILEWLAPVAHDTIRWQNERGFEQYRQIGKISTNVALVQTLY 383
             SIYDA +   WK+  D IL WLAP+AH+ IRWQ+ER FEQ+ QI K  TNV L+QTLY
Sbjct: 371 NISIYDAPLAHDWKETIDGILSWLAPLAHNMIRWQSERNFEQHNQIVK-RTNVLLLQTLY 430

BLAST of Spg031462 vs. NCBI nr
Match: XP_022138835.1 (uncharacterized protein LOC111009906 [Momordica charantia])

HSP 1 Score: 369.8 bits (948), Expect = 2.9e-98
Identity = 227/564 (40.25%), Postives = 296/564 (52.48%), Query Frame = 0

Query: 1   MGNSPSSNLPH-------------KP--PPNRTATIGILSFEIANVISRAIHLHNTLKHL 60
           MGN  SSNL H             KP    N   TIGILSFE+ANV+S+ I+LH +L H 
Sbjct: 10  MGNQVSSNLKHALLEPSKNNKNPKKPEISSNTKQTIGILSFEVANVMSKTIYLHKSLSHS 69

Query: 61  QFSNLRTQILTAEGITTLISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGF 120
             S L+ +IL+++G+  L+SSDE  LL L +AEK+ DLN+VA  VSRLGK+CS PALQGF
Sbjct: 70  AISKLKNEILSSDGVKNLVSSDELHLLELAVAEKLEDLNRVANVVSRLGKKCSQPALQGF 129

Query: 121 EHIYADVVSGNLEGKKLGILTKDMDGIVKKMESYVKRTANLYSAIEV------------S 180
           +H+Y D+V+G +  K+LG L KDM+G+++KME YV  TANLY+ +EV            +
Sbjct: 130 QHVYLDIVNGVINVKELGFLVKDMEGMMRKMERYVNATANLYTEMEVLNELEQAAKKFQN 189

Query: 181 NGHK-----------WQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVFHDH 240
           N H+           WQ K  + HLK ISLWN++Y+KVV+LL + V  +YARI LVF D 
Sbjct: 190 NQHEESKKAYEQKLVWQ-KQGVGHLKEISLWNQTYDKVVELLARTVCTVYARIHLVFGDS 249

Query: 241 LAGTDVKSSI-------------------------------------------------- 300
               DV  ++                                                  
Sbjct: 250 FLKKDVNENVVEPLESKRASIDKCPRPRRGSSFKSRVSLRRGEVPLFTPDDFNFPCGTNP 309

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 310 GRLLMDCLSLSSSVSKLDDEDEDEDGYADRDDRSCQISGRSIRNSGSSHFSSFSQVQFSV 369

Query: 361 ----------------------------HADSSTVGGAALTVRYANIIIITEKFLRQPHL 389
                                       +A  STVGG+AL + YANIII+ EK LR PHL
Sbjct: 370 PFGVDQRQNNSVMSNSGGNFGFKSRLSGYAPVSTVGGSALALHYANIIIVIEKLLRYPHL 429

BLAST of Spg031462 vs. NCBI nr
Match: XP_018486131.1 (PREDICTED: uncharacterized protein LOC108856751 [Raphanus sativus])

HSP 1 Score: 368.6 bits (945), Expect = 6.5e-98
Identity = 207/468 (44.23%), Postives = 283/468 (60.47%), Query Frame = 0

Query: 5   PSSNLPHKPPPNRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTLI 64
           PSS+    P P +  TIGILSFE+AN++S+ +HLH +L     SNL++ +  ++G+TTL+
Sbjct: 22  PSSSNKKTPLPKQ--TIGILSFEVANIMSKTVHLHRSLSDTDLSNLKSHVFLSQGVTTLV 81

Query: 65  SSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLGI 124
           SSD N LL L ++EK+ DL +VA  VSRLGK+C+ PALQGFEH+Y D+V+G ++ +KLG 
Sbjct: 82  SSDVNHLLDLAVSEKLDDLTRVATVVSRLGKKCNEPALQGFEHVYEDIVNGAIDFRKLGF 141

Query: 125 LTKDMDGIVKKMESYVKRTANLYSAIEVSN----------------GHK----------- 184
           L KDM+ +VKKME +V  T +LY  +EV N                 H+           
Sbjct: 142 LVKDMESMVKKMERFVNATCSLYCEMEVMNELEQAVLKLRRSGQGQQHQGSVKAFEQKLM 201

Query: 185 WQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVF-----------------H 244
           WQ + ++K L+  SLWN++Y+KVV++L + V  +Y RI  VF                  
Sbjct: 202 WQ-RQDVKSLRDASLWNQTYDKVVEMLARTVCTIYGRIETVFGGLGLRGRKDSSLKREVE 261

Query: 245 DHLAGTD------------------VKSSI----------------------------HA 304
           +   G D                  + SS+                            HA
Sbjct: 262 EFTRGADFSFPCGTNPGRMFMECLAMNSSVSRSYDEEVITFPRTMKSTKFGSKSRLTQHA 321

Query: 305 DSSTVGGAALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNAR 364
            +STVGG+AL++ YAN++I+ EK L+ PHL+G +ARDDLY+ML TSLK+SL+ +L+   +
Sbjct: 322 SASTVGGSALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSLKTSLKANLRSYLK 381

Query: 365 AQSIYDATVCRYWKQAADRILEWLAPVAHDTIRWQNERGFEQYRQIGKISTNVALVQTLY 383
             SIYDA +   WK+  D IL WLAP+AH+ IRWQ+ER FEQ+ QI K  TNV L+QTLY
Sbjct: 382 NISIYDAPLAHDWKETIDGILSWLAPLAHNMIRWQSERNFEQHNQIVK-RTNVLLLQTLY 441

BLAST of Spg031462 vs. NCBI nr
Match: KAF8098691.1 (hypothetical protein N665_0260s0001 [Sinapis alba])

HSP 1 Score: 367.9 bits (943), Expect = 1.1e-97
Identity = 208/479 (43.42%), Postives = 285/479 (59.50%), Query Frame = 0

Query: 1   MGNSPSSNLPH---------KPPPNRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLR 60
           M N  S NL H         K P     TIGILSFE+ANV+S+ +HLH +L   + S L+
Sbjct: 10  MRNQVSINLKHALLLESSSSKKPSFPKQTIGILSFEVANVMSKTVHLHRSLSDTELSKLK 69

Query: 61  TQILTAEGITTLISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYAD 120
           +++  ++G+  L+SSDEN LL L ++EK+ DL++VA  VSRLGK+C+ PALQGFEH+Y D
Sbjct: 70  SEVFLSQGVIKLVSSDENHLLDLAVSEKLDDLSRVASVVSRLGKKCNEPALQGFEHVYED 129

Query: 121 VVSGNLEGKKLGILTKDMDGIVKKMESYVKRTANLYSAIEV-------------SNGHK- 180
           +V+G ++ +KLG L KDM+ +VKKME +V  T NLY  +EV             S  H+ 
Sbjct: 130 IVNGVIDFRKLGFLVKDMESMVKKMERFVNATCNLYCEMEVMSELEQAVMKLQRSGQHQG 189

Query: 181 ----------WQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVF-------- 240
                     WQ + ++K L+  SLWN++Y+KVV++L + V  +Y RI  VF        
Sbjct: 190 SVKAFEQKLMWQ-RQDVKSLRDASLWNQTYDKVVEMLARTVCTIYGRIETVFGGLALRGK 249

Query: 241 -------------------------------------------HDHLA------------ 300
                                                      +D  A            
Sbjct: 250 KDSALKREAEEFTREADFSFPCGTNPGRMFMECLPMNSSVSRSYDDDARITFPRAMRSSK 309

Query: 301 -GTDVKSSIHADSSTVGGAALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKS 360
            G+  + + HA +ST+GG+AL++ YAN++I+ EK L+ PHL+G +ARDDLY+ML TSLK+
Sbjct: 310 FGSKSRLTQHASASTIGGSALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSLKT 369

Query: 361 SLRTHLKCNARAQSIYDATVCRYWKQAADRILEWLAPVAHDTIRWQNERGFEQYRQIGKI 383
           SL+ +L+   +  SIYDA +   WK+  D IL WLAP+AH+ IRWQ+ER FEQ+ QI K 
Sbjct: 370 SLKANLRSYLKNISIYDAPLAHDWKETIDGILSWLAPLAHNMIRWQSERNFEQHNQIVK- 429

BLAST of Spg031462 vs. NCBI nr
Match: KAG5378480.1 (hypothetical protein IGI04_026322 [Brassica rapa subsp. trilocularis])

HSP 1 Score: 367.1 bits (941), Expect = 1.9e-97
Identity = 203/461 (44.03%), Postives = 275/461 (59.65%), Query Frame = 0

Query: 13  PPPNRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTLISSDENRLL 72
           PPP    TIGILSFE+AN++S+ +HLH +L     SNL++ +  + G++TL+SSD N LL
Sbjct: 31  PPPLPKQTIGILSFEVANIMSKTVHLHRSLSDSDLSNLKSHVFPSPGVSTLVSSDSNHLL 90

Query: 73  RLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLGILTKDMDGI 132
            L ++EK+ DL++VA  VSRLGK+C+ PALQGFEH+Y D+VSG ++ +K+G L KDM+ +
Sbjct: 91  DLAVSEKLDDLSRVASVVSRLGKKCTEPALQGFEHVYEDIVSGVIDFRKVGFLVKDMESM 150

Query: 133 VKKMESYVKRTANLYSAIEVSN-------------GHK-----------WQTKNNIKHLK 192
           VKKME  V  T  LY  +EV N              H+           WQ + ++K L+
Sbjct: 151 VKKMERLVNATCTLYCEMEVMNELEQAVVKLRRSGQHQGSVKAFEQKLMWQ-RQDVKSLR 210

Query: 193 HISLWNKSYNKVVQLLGKMVVLLYARISLVF----------------------------- 252
             SLWN++Y+KVV++L + V  +Y RI  VF                             
Sbjct: 211 EASLWNQTYDKVVEMLARTVCTIYGRIETVFGGLGVRGKKDSSLKRREAEGDDFSFPCGT 270

Query: 253 ------------------HD--------------------HLAGTDVKSSIHADSSTVGG 312
                             HD                       G+  + + HA  STVGG
Sbjct: 271 NPGRMFMECLPMNVSSRSHDDDDEEEEEARSITFPRGMRSSKFGSKSRLTQHASPSTVGG 330

Query: 313 AALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNARAQSIYDA 372
           +AL++ YAN++I+ EK L+ PHL+G +ARDDLY+ML TSLK+SL+ +L+   +  SIYDA
Sbjct: 331 SALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSLKTSLKANLRSYLKNVSIYDA 390

Query: 373 TVCRYWKQAADRILEWLAPVAHDTIRWQNERGFEQYRQIGKISTNVALVQTLYFADREKT 383
            +   WK+  D IL WLAP+AH+ IRWQ+ER FEQ+ QI K  TNV L+QTLYFADREKT
Sbjct: 391 PLAHDWKETIDGILSWLAPLAHNMIRWQSERNFEQHNQIVK-RTNVLLLQTLYFADREKT 450

BLAST of Spg031462 vs. ExPASy Swiss-Prot
Match: Q9XID5 (Protein PSK SIMULATOR 1 OS=Arabidopsis thaliana OX=3702 GN=PSI1 PE=1 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 4.8e-27
Identity = 102/379 (26.91%), Postives = 174/379 (45.91%), Query Frame = 0

Query: 21  IGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTLISSDENRLLRLVLAEKV 80
           I ILSFE+AN I +  +L ++L     ++L+  +L +EG+  LIS D + LLR+  A+K 
Sbjct: 155 ISILSFEVANTIVKGANLMHSLSKDSITHLKEVVLPSEGVQNLISKDMDELLRIAAADKR 214

Query: 81  HDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLGILTKDMDGIVKKMESYV 140
            +L   +G V R G RC  P     +  + D +      +K   L ++ + I+ +M S+V
Sbjct: 215 EELRIFSGEVVRFGNRCKDPQYHNLDRFF-DRLGSEFTPQK--HLKQEAETIMHQMMSFV 274

Query: 141 KRTANLYSAI---------------EVSNGHKWQ----------------TKNNIKHLKH 200
             TA+LY  +               E  N    Q                 K ++++LK 
Sbjct: 275 HFTADLYHELHALDRFEQDYQRKIQEEENPSTAQRGVGDTLAILRTELKSQKKHVRNLKK 334

Query: 201 ISLWNKSYNKVVQLLGKMVVLLYARISLVFHDHLAGTDVKSSIH---ADSSTVGGAALTV 260
            SLW++   +V++ L  +V  L+  I    H+   G D     +    +   +G A L +
Sbjct: 335 KSLWSRILEEVMEKLVDVVHFLHLEI----HEAFGGADPDKPANDPPINHKKLGSAGLAL 394

Query: 261 RYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNARAQSIYDATVCRY 320
            YANII   +  + +   +    RD LY+ L  S+KS+LR+ +    ++  + +      
Sbjct: 395 HYANIITQIDTLVSRSSTMPASTRDALYQGLPPSIKSALRSRI----QSFQVKEELTVPQ 454

Query: 321 WKQAADRILEWLAPVAHDTIR----------WQNERGFEQYRQIGKISTNVALVQTLYFA 356
            K   ++ L+WL PVA +T +          W +       R  G+    +  + TL+ A
Sbjct: 455 IKAEMEKTLQWLVPVATNTTKAHHGFGWVGEWASSGSEANQRPAGQ---TILRIDTLHHA 514

BLAST of Spg031462 vs. ExPASy Swiss-Prot
Match: P0DO24 (Protein PSK SIMULATOR 3 OS=Arabidopsis thaliana OX=3702 GN=PSI3 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 3.4e-25
Identity = 101/371 (27.22%), Postives = 173/371 (46.63%), Query Frame = 0

Query: 21  IGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTLISSDENRLLRLVLAEKV 80
           +GIL+FE+AN I ++ +L  +L      +L+  IL +EG+  L+S+D + LLRLV A+K 
Sbjct: 149 LGILAFEVANTIVKSSNLIESLSKRNIEHLKGTILYSEGVQNLVSNDFDELLRLVAADKR 208

Query: 81  HDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLGILTKDMDGIVKKMESYV 140
            +L   +G V R G R         +  Y D +S  L  ++   L +D   +V ++   V
Sbjct: 209 QELQVFSGEVVRFGNRSKDFQWHNLQR-YFDRISKELTPQRQ--LKEDAVLVVDQLMVLV 268

Query: 141 KRTANLYSAIEV------------------SNGHKWQ-----------TKNNIKHLKHIS 200
           + TA LY  ++V                  +N  K              +  +K LK  S
Sbjct: 269 QYTAELYQELQVLYRLEKDYEQKRREEENSANSSKGDGLAILKTELKAQRKVVKSLKKKS 328

Query: 201 LWNKSYNKVVQLLGKMVVLLYARISLVFHDHLAGTDVKSSIHADSSTVGGAALTVRYANI 260
           LW++ + +V++ L  +V  L   I  +F         K +   D   +G A L + YANI
Sbjct: 329 LWSRGFEEVMEKLVDIVHFLLLEIHNIFGGADDQPSKKGAAEYD-KRLGPAGLALHYANI 388

Query: 261 IIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNARAQSIYDATVCRYWKQAA 320
           I+  +  + +   + ++ARD LY+ L   +K +LR+ +K     + +    +    K   
Sbjct: 389 IVQIDTLVARASSITSNARDSLYQSLPPGIKLALRSKIKSFNVDKELSVTQI----KDEM 448

Query: 321 DRILEWLAPVAHDTIRWQNERGF-EQYRQIGKIST------NVALVQTLYFADREKTEAS 356
           +R L WL PVA +T +  +  G+  ++   G   T      ++  ++TLY A +EKTE  
Sbjct: 449 ERTLHWLVPVAGNTTKAHHGFGWVGEWANTGTDFTSKPSGGDILRIETLYHASKEKTEIY 508

BLAST of Spg031462 vs. ExPASy TrEMBL
Match: A0A6J1CAM1 (uncharacterized protein LOC111009906 OS=Momordica charantia OX=3673 GN=LOC111009906 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 1.4e-98
Identity = 227/564 (40.25%), Postives = 296/564 (52.48%), Query Frame = 0

Query: 1   MGNSPSSNLPH-------------KP--PPNRTATIGILSFEIANVISRAIHLHNTLKHL 60
           MGN  SSNL H             KP    N   TIGILSFE+ANV+S+ I+LH +L H 
Sbjct: 10  MGNQVSSNLKHALLEPSKNNKNPKKPEISSNTKQTIGILSFEVANVMSKTIYLHKSLSHS 69

Query: 61  QFSNLRTQILTAEGITTLISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGF 120
             S L+ +IL+++G+  L+SSDE  LL L +AEK+ DLN+VA  VSRLGK+CS PALQGF
Sbjct: 70  AISKLKNEILSSDGVKNLVSSDELHLLELAVAEKLEDLNRVANVVSRLGKKCSQPALQGF 129

Query: 121 EHIYADVVSGNLEGKKLGILTKDMDGIVKKMESYVKRTANLYSAIEV------------S 180
           +H+Y D+V+G +  K+LG L KDM+G+++KME YV  TANLY+ +EV            +
Sbjct: 130 QHVYLDIVNGVINVKELGFLVKDMEGMMRKMERYVNATANLYTEMEVLNELEQAAKKFQN 189

Query: 181 NGHK-----------WQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVFHDH 240
           N H+           WQ K  + HLK ISLWN++Y+KVV+LL + V  +YARI LVF D 
Sbjct: 190 NQHEESKKAYEQKLVWQ-KQGVGHLKEISLWNQTYDKVVELLARTVCTVYARIHLVFGDS 249

Query: 241 LAGTDVKSSI-------------------------------------------------- 300
               DV  ++                                                  
Sbjct: 250 FLKKDVNENVVEPLESKRASIDKCPRPRRGSSFKSRVSLRRGEVPLFTPDDFNFPCGTNP 309

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 310 GRLLMDCLSLSSSVSKLDDEDEDEDGYADRDDRSCQISGRSIRNSGSSHFSSFSQVQFSV 369

Query: 361 ----------------------------HADSSTVGGAALTVRYANIIIITEKFLRQPHL 389
                                       +A  STVGG+AL + YANIII+ EK LR PHL
Sbjct: 370 PFGVDQRQNNSVMSNSGGNFGFKSRLSGYAPVSTVGGSALALHYANIIIVIEKLLRYPHL 429

BLAST of Spg031462 vs. ExPASy TrEMBL
Match: A0A6J0NPR6 (uncharacterized protein LOC108856751 OS=Raphanus sativus OX=3726 GN=LOC108856751 PE=4 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 3.2e-98
Identity = 207/468 (44.23%), Postives = 283/468 (60.47%), Query Frame = 0

Query: 5   PSSNLPHKPPPNRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTLI 64
           PSS+    P P +  TIGILSFE+AN++S+ +HLH +L     SNL++ +  ++G+TTL+
Sbjct: 22  PSSSNKKTPLPKQ--TIGILSFEVANIMSKTVHLHRSLSDTDLSNLKSHVFLSQGVTTLV 81

Query: 65  SSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLGI 124
           SSD N LL L ++EK+ DL +VA  VSRLGK+C+ PALQGFEH+Y D+V+G ++ +KLG 
Sbjct: 82  SSDVNHLLDLAVSEKLDDLTRVATVVSRLGKKCNEPALQGFEHVYEDIVNGAIDFRKLGF 141

Query: 125 LTKDMDGIVKKMESYVKRTANLYSAIEVSN----------------GHK----------- 184
           L KDM+ +VKKME +V  T +LY  +EV N                 H+           
Sbjct: 142 LVKDMESMVKKMERFVNATCSLYCEMEVMNELEQAVLKLRRSGQGQQHQGSVKAFEQKLM 201

Query: 185 WQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVF-----------------H 244
           WQ + ++K L+  SLWN++Y+KVV++L + V  +Y RI  VF                  
Sbjct: 202 WQ-RQDVKSLRDASLWNQTYDKVVEMLARTVCTIYGRIETVFGGLGLRGRKDSSLKREVE 261

Query: 245 DHLAGTD------------------VKSSI----------------------------HA 304
           +   G D                  + SS+                            HA
Sbjct: 262 EFTRGADFSFPCGTNPGRMFMECLAMNSSVSRSYDEEVITFPRTMKSTKFGSKSRLTQHA 321

Query: 305 DSSTVGGAALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNAR 364
            +STVGG+AL++ YAN++I+ EK L+ PHL+G +ARDDLY+ML TSLK+SL+ +L+   +
Sbjct: 322 SASTVGGSALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSLKTSLKANLRSYLK 381

Query: 365 AQSIYDATVCRYWKQAADRILEWLAPVAHDTIRWQNERGFEQYRQIGKISTNVALVQTLY 383
             SIYDA +   WK+  D IL WLAP+AH+ IRWQ+ER FEQ+ QI K  TNV L+QTLY
Sbjct: 382 NISIYDAPLAHDWKETIDGILSWLAPLAHNMIRWQSERNFEQHNQIVK-RTNVLLLQTLY 441

BLAST of Spg031462 vs. ExPASy TrEMBL
Match: A0A6J1H3Z9 (uncharacterized protein LOC111459942 OS=Cucurbita moschata OX=3662 GN=LOC111459942 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 1.2e-97
Identity = 218/526 (41.44%), Postives = 297/526 (56.46%), Query Frame = 0

Query: 1   MGNSPSSNL--------PHKPPPNRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLRT 60
           MGN  S+NL         +K   N   TIGILSFE+AN++S+ I+LH +L     S L+ 
Sbjct: 10  MGNQVSANLKQALLEPSKNKNNGNNKQTIGILSFEVANIMSKTIYLHKSLSDSAISKLKN 69

Query: 61  QILTAEGITTLISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADV 120
           +IL+++G+  L+SSD+  LL LV+ EK+ DLN+VA  VSR G++CS PALQGF+H+Y D+
Sbjct: 70  EILSSDGVKNLVSSDDVHLLELVVLEKLEDLNRVANVVSRFGRKCSQPALQGFKHVYLDI 129

Query: 121 VSGNLEGKKLGILTKDMDGIVKKMESYVKRTANLYSAIEV------------SNGHK--- 180
           ++G +  K+LG L KDM+G+++KME +V  TANLY+ +EV            +N H+   
Sbjct: 130 INGAINVKELGFLVKDMEGMMRKMERFVNVTANLYTEMEVLNELEQAAKKFQNNQHEQTR 189

Query: 181 --------WQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARI-------------- 240
                   WQ K ++ HLK ISLWN++++KVV+LL + V  +YARI              
Sbjct: 190 KAYEQKLLWQ-KQDVGHLKEISLWNQTFDKVVELLVRTVCTVYARIHHVRIDAESMESKR 249

Query: 241 ---------------------------------------------------------SLV 300
                                                                    S V
Sbjct: 250 VFIERGHGARRGSSFKSQVSSKRSEVPLFDDFNFPCGTNPGRVLMDCLSLSSSGDEDSYV 309

Query: 301 FHDHLA----------------------GTDVKS--------------SIHADSSTVGGA 360
            HD L+                      G D++               S++A  STVGG+
Sbjct: 310 DHDDLSCQRSGRSIRNSGSSQVQFSVPFGVDLRQANNGGNFGFKSRLLSVYAPVSTVGGS 369

Query: 361 ALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNARAQSIYDAT 389
           AL + YANIII+ EK LR PHLVG++ARDDLYEML TSL+SSL+THLK   +  +IYDA 
Sbjct: 370 ALALHYANIIIVIEKLLRYPHLVGDEARDDLYEMLPTSLRSSLKTHLKSYVKNMAIYDAP 429

BLAST of Spg031462 vs. ExPASy TrEMBL
Match: A0A3P6B3U1 (Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRAA07T28732Z PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 1.6e-97
Identity = 205/470 (43.62%), Postives = 276/470 (58.72%), Query Frame = 0

Query: 4   SPSSNLPHKPPPNRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTL 63
           S     P  PPP    TIGILSFE+ANV+S+ +HLH +L     SNL + +  + G++TL
Sbjct: 23  SSKKGAPPPPPPLPKQTIGILSFEVANVMSKTVHLHRSLSDSDLSNLTSHVFPSPGVSTL 82

Query: 64  ISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLG 123
           +SSD N LL L ++EK+ DL++VA  VSRLGK+C+ PALQGFEH+Y D+VSG ++ +K+G
Sbjct: 83  VSSDSNHLLDLAVSEKLDDLSRVASVVSRLGKKCTEPALQGFEHVYEDIVSGVIDFRKVG 142

Query: 124 ILTKDMDGIVKKMESYVKRTANLYSAIEVSN-------------GHK-----------WQ 183
            L KDM+ +VKKME  V  T  LY  +EV N              H+           WQ
Sbjct: 143 FLVKDMESMVKKMERLVNATCTLYCEMEVMNELEQAVVKLRRSGQHQGSVKAFEQKLMWQ 202

Query: 184 TKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVF-------------------- 243
            + +++ L+  SLWN++Y+KVV++L + V  +Y RI  VF                    
Sbjct: 203 -RQDVRSLREASLWNQTYDKVVEMLARTVCTIYGRIESVFGGLGVRGKKDSSLKRREAEG 262

Query: 244 ---------------------------HD--------------------HLAGTDVKSSI 303
                                      HD                       G+  + + 
Sbjct: 263 ADFSFPCGTNPGRMFMECLPMNVSSRSHDDDDEEEEEARSITFPRGMRSSKFGSKSRLTQ 322

Query: 304 HADSSTVGGAALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCN 363
           HA  STVGG+AL++ YAN++I+ EK L+ PHL+G +ARDDLY+ML TSLK+SL+ +L+  
Sbjct: 323 HASPSTVGGSALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSLKTSLKANLRSY 382

Query: 364 ARAQSIYDATVCRYWKQAADRILEWLAPVAHDTIRWQNERGFEQYRQIGKISTNVALVQT 383
            +  SIYDA +   WK+  D IL WLAP+AH+ IRWQ+ER FEQ+ QI K  TNV L+QT
Sbjct: 383 LKNVSIYDAPLAHDWKETIDGILSWLAPLAHNMIRWQSERNFEQHNQIVK-RTNVLLLQT 442

BLAST of Spg031462 vs. ExPASy TrEMBL
Match: A0A3P6EFT0 (Uncharacterized protein OS=Brassica oleracea OX=3712 GN=BOLC7T41936H PE=4 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 3.5e-97
Identity = 206/481 (42.83%), Postives = 280/481 (58.21%), Query Frame = 0

Query: 6   SSNLPH--------KPPPNRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTA 65
           S+NL H        K  P    TIGILSFE+AN++S+ +HLH +L   + S L++ +  +
Sbjct: 11  STNLKHALLLEPSSKKAPLPKQTIGILSFEVANIMSKTVHLHRSLSDSELSKLKSDVFLS 70

Query: 66  EGITTLISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNL 125
           +G+T L+SSD N LL L ++EK+ DL++VA  VSRLGK+C+ PALQGFEH+Y D+V+G +
Sbjct: 71  QGVTKLVSSDSNHLLDLAVSEKLDDLSRVASVVSRLGKKCNEPALQGFEHVYEDIVNGVI 130

Query: 126 EGKKLGILTKDMDGIVKKMESYVKRTANLYSAIEV------------SNGH--------- 185
           + +KLG L KDM+ +VKKME +V  T  LY  +EV              GH         
Sbjct: 131 DFRKLGFLVKDMESMVKKMERFVNATCTLYCEMEVLTELEQAVVKLQRTGHHQGSVKAFE 190

Query: 186 ---KWQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVF-------------- 245
               WQ + ++K L+  SLWN++Y+KVV++L + V  +Y RI  VF              
Sbjct: 191 QKLMWQ-RQDVKSLREASLWNQTYDKVVEMLARTVCTIYGRIETVFGGLGLRGKKDSTLK 250

Query: 246 ---------------------------------------HDHLAGTDVKSSI-------- 305
                                                  HD    ++   SI        
Sbjct: 251 RREAEGFTREADFSFPCGTNPGRMFMECLPMNSSVSSRSHDDEEDSEEARSITFPRAMRS 310

Query: 306 -----------HADSSTVGGAALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSL 365
                      HA  ST+GG+AL++ YAN++I+ EK L+ PHL+G +ARDDLY+ML TSL
Sbjct: 311 SKFGSKSRLTQHASPSTIGGSALSLHYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSL 370

Query: 366 KSSLRTHLKCNARAQSIYDATVCRYWKQAADRILEWLAPVAHDTIRWQNERGFEQYRQIG 383
           K+SL+ +L+   +  SIYDA +   WK+A D IL WLAP+AH+ IRWQ+ER FEQ+ QI 
Sbjct: 371 KTSLKANLRSYLKNVSIYDAPLAHDWKEAIDGILSWLAPLAHNMIRWQSERNFEQHNQIV 430

BLAST of Spg031462 vs. TAIR 10
Match: AT3G23160.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 349.4 bits (895), Expect = 3.8e-96
Identity = 207/516 (40.12%), Postives = 280/516 (54.26%), Query Frame = 0

Query: 1   MGNSPSSNLPH-------------KPPPNRTATIGILSFEIANVISRAIHLHNTLKHLQF 60
           M N  SSNL H             KP      TIGILSFE+ANV+S+ IHLH +L   + 
Sbjct: 10  MRNQVSSNLKHALLLESSSTKKTPKPVSLPKQTIGILSFEVANVMSKTIHLHRSLSDTEI 69

Query: 61  SNLRTQILTAEGITTLISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEH 120
           S L+ ++  +EG+  L+SSDEN LL L ++EK+ DL++VA  VSRLGK+C+ PALQGFEH
Sbjct: 70  SKLKAEVFHSEGVRKLVSSDENHLLDLSVSEKLDDLSRVASVVSRLGKKCNEPALQGFEH 129

Query: 121 IYADVVSGNLEGKKLGILTKDMDGIVKKMESYVKRTANLYSAIEVSN------------- 180
           +Y D+V+G ++ +KLG L KDM+ +VKKME +V  T +LY  +EV N             
Sbjct: 130 VYEDIVNGAIDFRKLGFLVKDMESMVKKMERFVNATCSLYCEMEVMNELEQAIVKLQRSQ 189

Query: 181 GHK-----------WQTKNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVF---- 240
            H+           WQ + ++K L+  SLWN++Y+KVV++L + V  +Y RI  VF    
Sbjct: 190 QHQESVKAFEQKLMWQ-RQDVKSLRDGSLWNQTYDKVVEMLARTVCTIYGRIETVFGGLG 249

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 250 LRGKKDVTLKRDRSKNEASKAVNSRSVAGFKDSRRSEADEFTRAGDFNFPCGTNPGRMFM 309

Query: 301 ---------------------------------HDHLAGTDVKSSIHADSSTVGGAALTV 360
                                              +  G   + + HA +ST+GG+AL++
Sbjct: 310 ECLAMNRTIGDDDDDDDEDGGRITFPLSTARMIRSNKFGFKSRLTQHASASTIGGSALSL 369

Query: 361 RYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNARAQSIYDATVCRY 383
            YAN++I+ EK L+ PHL+G +ARDDLY+ML TSLK++L+  L+   +  SIYDA +   
Sbjct: 370 HYANVVIVVEKLLKYPHLIGEEARDDLYQMLPTSLKTTLKASLRSYLKNISIYDAPLAHD 429

BLAST of Spg031462 vs. TAIR 10
Match: AT5G51670.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 216.9 bits (551), Expect = 3.0e-56
Identity = 149/449 (33.18%), Postives = 220/449 (49.00%), Query Frame = 0

Query: 1   MGNSPSSNLPHKPPP---NRTATIGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTA 60
           + + P+S  PH+  P     T+++G+LSFE+A V+++ +HL ++L        R   L+ 
Sbjct: 14  ISSKPTSRRPHRSSPPISTTTSSVGVLSFEVARVMTKLLHLTHSLTDSNLLTPRDHSLSL 73

Query: 61  EGITTLISSDENRLLRLVLAEKVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNL 120
           EG+T +++ DE   L LV AE    L   A SVSRL  RC+  +L+ F  ++ +      
Sbjct: 74  EGLTKIVNGDETFHLSLVCAELADSLAHAANSVSRLSNRCTTASLRSFHRLFHEFADMGR 133

Query: 121 EGKKLGILTKDMDGIVKKMESYVKRTANLYSAIE----VSNGHKWQT------------- 180
           +     +  KD +   KK+E YV  T  LY  +E    + N  + Q+             
Sbjct: 134 DPHGWVMNCKDTEAKNKKIERYVSVTTALYREMEEMAILENSLRKQSLQIGIEFEEEEDY 193

Query: 181 -------------------KNNIKHLKHISLWNKSYNKVVQLLGKMVVLLYARISLVFHD 240
                              K ++K+LK  SLWNKS++ VV +L + V    AR+  VF  
Sbjct: 194 ENKKDVMKVIDLQNKIERQKQHVKYLKDRSLWNKSFDTVVLILARSVFTALARLKSVFSS 253

Query: 241 -----HLAGTDVKS---SIHADSS------------------------------------ 300
                ++  T V S   S+ + SS                                    
Sbjct: 254 AAATGYMVPTVVSSLPRSLSSSSSSMNLVHPSPNDEERDKTTTSSAFLEESSRLLKPPET 313

Query: 301 TVGGAALTVRYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNARAQS 360
           T+GGA + + YAN+I++ EK ++QP LVG DARDDLY ML  S++SSLR+ LK      +
Sbjct: 314 TLGGAGVALHYANLIVVMEKMIKQPQLVGLDARDDLYSMLPASVRSSLRSRLK--GVGFT 373

Query: 361 IYDATVCRYWKQAADRILEWLAPVAHDTIRWQNERGFEQYRQIGKIST--NVALVQTLYF 365
             D  +   WK A  RIL WL P+A + IRWQ+ER FEQ       ++   V LVQTL F
Sbjct: 374 ATDGGLATEWKAALGRILRWLLPLAQNMIRWQSERSFEQQHMATATNSQNRVMLVQTLVF 433

BLAST of Spg031462 vs. TAIR 10
Match: AT5G04550.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 174.9 bits (442), Expect = 1.3e-43
Identity = 140/567 (24.69%), Postives = 231/567 (40.74%), Query Frame = 0

Query: 19  ATIGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTLISSDENRLLRLVLAE 78
           A +G+L+FE+A+++S+ +HL  +L     + LR +I  + GI  L+S D++ ++RL+  E
Sbjct: 24  AVLGVLAFEVASLLSKLVHLWQSLSDKNVARLRDEITHSTGIKKLVSEDDDFIVRLIRDE 83

Query: 79  KVHDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLGILTKDMDGIVKKMES 138
            + ++  VA +V+RL ++C+ P L+ FE+ ++D++    +        K MD   KKME 
Sbjct: 84  MMENVENVAKAVARLARKCNDPKLKCFENCFSDMMKTGADPYGWQFGWKKMDKKAKKMER 143

Query: 139 YVKRTANLYSAIEV------------------SNGHKWQTK-----NNIKHLKHISLWNK 198
           ++   A+LY   E+                   N  ++Q K     + +K+L+ +SLWN+
Sbjct: 144 FISSNASLYQETEILADLEQTFKRMKSNESATDNLLEYQKKVTWKRHEVKNLRDVSLWNR 203

Query: 199 SYNKVVQLLGKMVVLLYARISLVF------------------------------------ 258
           +Y+  V LL + V  + +R   VF                                    
Sbjct: 204 TYDYTVILLVRSVFTILSRTKHVFGISYRVEASDVSSADSDFIGRSHSVSTILTPVSHKS 263

Query: 259 -------------------------------HDHLAGT---------------------- 318
                                           D L+G+                      
Sbjct: 264 ESSGLPRFASGPLGRFTGPASGSAATRSTKMSDFLSGSLSAESPKSGPLVAEKHKRFKFY 323

Query: 319 ------------------------------------------------------------ 372
                                                                       
Sbjct: 324 SGQLGKITSKSGPLIGMGKNNKKMGQTPERPSISSVKKQLKANRLTQVGPFKGCMVSHDG 383

BLAST of Spg031462 vs. TAIR 10
Match: AT1G34320.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 123.6 bits (309), Expect = 3.4e-28
Identity = 102/379 (26.91%), Postives = 174/379 (45.91%), Query Frame = 0

Query: 21  IGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTLISSDENRLLRLVLAEKV 80
           I ILSFE+AN I +  +L ++L     ++L+  +L +EG+  LIS D + LLR+  A+K 
Sbjct: 155 ISILSFEVANTIVKGANLMHSLSKDSITHLKEVVLPSEGVQNLISKDMDELLRIAAADKR 214

Query: 81  HDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLGILTKDMDGIVKKMESYV 140
            +L   +G V R G RC  P     +  + D +      +K   L ++ + I+ +M S+V
Sbjct: 215 EELRIFSGEVVRFGNRCKDPQYHNLDRFF-DRLGSEFTPQK--HLKQEAETIMHQMMSFV 274

Query: 141 KRTANLYSAI---------------EVSNGHKWQ----------------TKNNIKHLKH 200
             TA+LY  +               E  N    Q                 K ++++LK 
Sbjct: 275 HFTADLYHELHALDRFEQDYQRKIQEEENPSTAQRGVGDTLAILRTELKSQKKHVRNLKK 334

Query: 201 ISLWNKSYNKVVQLLGKMVVLLYARISLVFHDHLAGTDVKSSIH---ADSSTVGGAALTV 260
            SLW++   +V++ L  +V  L+  I    H+   G D     +    +   +G A L +
Sbjct: 335 KSLWSRILEEVMEKLVDVVHFLHLEI----HEAFGGADPDKPANDPPINHKKLGSAGLAL 394

Query: 261 RYANIIIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNARAQSIYDATVCRY 320
            YANII   +  + +   +    RD LY+ L  S+KS+LR+ +    ++  + +      
Sbjct: 395 HYANIITQIDTLVSRSSTMPASTRDALYQGLPPSIKSALRSRI----QSFQVKEELTVPQ 454

Query: 321 WKQAADRILEWLAPVAHDTIR----------WQNERGFEQYRQIGKISTNVALVQTLYFA 356
            K   ++ L+WL PVA +T +          W +       R  G+    +  + TL+ A
Sbjct: 455 IKAEMEKTLQWLVPVATNTTKAHHGFGWVGEWASSGSEANQRPAGQ---TILRIDTLHHA 514

BLAST of Spg031462 vs. TAIR 10
Match: AT5G08660.1 (Protein of unknown function (DUF668) )

HSP 1 Score: 117.5 bits (293), Expect = 2.4e-26
Identity = 101/371 (27.22%), Postives = 173/371 (46.63%), Query Frame = 0

Query: 21  IGILSFEIANVISRAIHLHNTLKHLQFSNLRTQILTAEGITTLISSDENRLLRLVLAEKV 80
           +GIL+FE+AN I ++ +L  +L      +L+  IL +EG+  L+S+D + LLRLV A+K 
Sbjct: 149 LGILAFEVANTIVKSSNLIESLSKRNIEHLKGTILYSEGVQNLVSNDFDELLRLVAADKR 208

Query: 81  HDLNQVAGSVSRLGKRCSVPALQGFEHIYADVVSGNLEGKKLGILTKDMDGIVKKMESYV 140
            +L   +G V R G R         +  Y D +S  L  ++   L +D   +V ++   V
Sbjct: 209 QELQVFSGEVVRFGNRSKDFQWHNLQR-YFDRISKELTPQRQ--LKEDAVLVVDQLMVLV 268

Query: 141 KRTANLYSAIEV------------------SNGHKWQ-----------TKNNIKHLKHIS 200
           + TA LY  ++V                  +N  K              +  +K LK  S
Sbjct: 269 QYTAELYQELQVLYRLEKDYEQKRREEENSANSSKGDGLAILKTELKAQRKVVKSLKKKS 328

Query: 201 LWNKSYNKVVQLLGKMVVLLYARISLVFHDHLAGTDVKSSIHADSSTVGGAALTVRYANI 260
           LW++ + +V++ L  +V  L   I  +F         K +   D   +G A L + YANI
Sbjct: 329 LWSRGFEEVMEKLVDIVHFLLLEIHNIFGGADDQPSKKGAAEYD-KRLGPAGLALHYANI 388

Query: 261 IIITEKFLRQPHLVGNDARDDLYEMLTTSLKSSLRTHLKCNARAQSIYDATVCRYWKQAA 320
           I+  +  + +   + ++ARD LY+ L   +K +LR+ +K     + +    +    K   
Sbjct: 389 IVQIDTLVARASSITSNARDSLYQSLPPGIKLALRSKIKSFNVDKELSVTQI----KDEM 448

Query: 321 DRILEWLAPVAHDTIRWQNERGF-EQYRQIGKIST------NVALVQTLYFADREKTEAS 356
           +R L WL PVA +T +  +  G+  ++   G   T      ++  ++TLY A +EKTE  
Sbjct: 449 ERTLHWLVPVAGNTTKAHHGFGWVGEWANTGTDFTSKPSGGDILRIETLYHASKEKTEIY 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG2306699.14.5e-9944.44hypothetical protein Bca52824_026447 [Brassica carinata][more]
XP_022138835.12.9e-9840.25uncharacterized protein LOC111009906 [Momordica charantia][more]
XP_018486131.16.5e-9844.23PREDICTED: uncharacterized protein LOC108856751 [Raphanus sativus][more]
KAF8098691.11.1e-9743.42hypothetical protein N665_0260s0001 [Sinapis alba][more]
KAG5378480.11.9e-9744.03hypothetical protein IGI04_026322 [Brassica rapa subsp. trilocularis][more]
Match NameE-valueIdentityDescription
Q9XID54.8e-2726.91Protein PSK SIMULATOR 1 OS=Arabidopsis thaliana OX=3702 GN=PSI1 PE=1 SV=2[more]
P0DO243.4e-2527.22Protein PSK SIMULATOR 3 OS=Arabidopsis thaliana OX=3702 GN=PSI3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CAM11.4e-9840.25uncharacterized protein LOC111009906 OS=Momordica charantia OX=3673 GN=LOC111009... [more]
A0A6J0NPR63.2e-9844.23uncharacterized protein LOC108856751 OS=Raphanus sativus OX=3726 GN=LOC108856751... [more]
A0A6J1H3Z91.2e-9741.44uncharacterized protein LOC111459942 OS=Cucurbita moschata OX=3662 GN=LOC1114599... [more]
A0A3P6B3U11.6e-9743.62Uncharacterized protein OS=Brassica campestris OX=3711 GN=BRAA07T28732Z PE=4 SV=... [more]
A0A3P6EFT03.5e-9742.83Uncharacterized protein OS=Brassica oleracea OX=3712 GN=BOLC7T41936H PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G23160.13.8e-9640.12Protein of unknown function (DUF668) [more]
AT5G51670.13.0e-5633.18Protein of unknown function (DUF668) [more]
AT5G04550.11.3e-4324.69Protein of unknown function (DUF668) [more]
AT1G34320.13.4e-2826.91Protein of unknown function (DUF668) [more]
AT5G08660.12.4e-2627.22Protein of unknown function (DUF668) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021864Domain of unknown function DUF3475PFAMPF11961DUF3475coord: 23..79
e-value: 2.4E-19
score: 69.1
IPR007700Domain of unknown function DUF668PFAMPF05003DUF668coord: 218..307
e-value: 5.4E-28
score: 97.3
NoneNo IPR availablePANTHERPTHR31371:SF2PLANT/PROTEIN (DUF668)coord: 209..384
coord: 12..207
NoneNo IPR availablePANTHERPTHR31371BNAC09G50660D PROTEINcoord: 209..384
coord: 12..207

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031462.1Spg031462.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045927 positive regulation of growth