Spg031450 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031450
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
DescriptionWRKY transcription factor
Locationscaffold11: 36917052 .. 36918975 (-)
RNA-Seq ExpressionSpg031450
SyntenySpg031450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTGTTTCTATGCCCACTGTTTACCGGCACCTGTCGGTCACCATCCTAGGTGGGAAAACTTACACCCCAAGCTGTTCTCCAAAATCCCAAACACTCTTTTCTTTTTCTCTTACACTCTCCAACAAAAGAATATTATTATTATTATTATGTCTTAAACAAAAAAAAAAAAAAATTCCGATCGATCCCAATGGAGAAGAAATTTGAAGACTCATTGATGGGTTCTGAAAATTCCGCCGGATTTTACTCTCCGGCCCTGTTCTCCGACGACTTTCCGGCCACTGGGTTTGGTTTTTCCGGTATGTTCGACATGCCATGTGACGATGATCATAAAGCTTCTTGTTCTTCGAATTCCTTTGGAATTGGAATCCACGATCTTTATAATCCTTCTTCTTTGTTCGATTTGCTTTCCACGGCGGCGCCGCCGCTGCAGCAGCCGCTTTCCTCGCCGGCCTCCACCGTGCCCGAGTCGTCCGAGGTGCTCAATGCTCCGGCCACGCCGAATTCTTCGTCGGTTTCGAATTCCTCCTCCAATGAAGCTGCGGCCATTGAAGAGGTCGACAAGAACAACAACACTGACAAGACTTCTAAAGTGTAAGAGATTATTGTTCGATCTCTCGTTTTGAATTTCGGGTTTAGATTTGTGAAATGTTGTAGTGGAATCGACCTGGGGTCGGGTTTTTTTTTTTTTTTTTTGTCTTCACAATAGTATGATATTGTCGATTTGGATCTCAACTCGAGATTTGAGATATTGTTGGTATTTTTGTACTATTGGTGTTTGTTTTTGTTGATGATGATGAGGAAATTATGGGGGAAAAGGCATGTGATTAATGGGGTTTTGTTGATTTGATTTAGTGGTGTAATTTTTGAGCTTGATGATTCTTCTTCTTTTTTTTGCTTCTTTTTGGACAAAAAGAAATGGAAAAAGTGAAGCTTTTTTTTTTTTTTGGGTGTGTGTTTGTTTTTGTTTTTAATATTGTTGTTGGGTTGGTTTTAGGTTGAAGCCAAAGAAGAAGAACCAGAAGAAGCAAAGAGAACCCAGATTTGCTTTCATGACAAAGAGTGATATCGATCATCTCGACGACGGTTACCGGTGGCGAAAGTACGGCCAAAAGGCCGTGAAGAACAGTCCTTATCCCAGGTTCGTAACGATACGATATTATTCACTTTGAATTAACTTTCTACGACTTTGATTTTAGTAAAAGGTATCGTACTAATGAAGATAGTTATATCATACAGAAGCTATTATCGTTGCACTACGGCAGGGTGTGGAGTGAAGAAGAGGGTGGAGCGGTCATCCGGCGACCATTCGATCGTCATCACAACGTACGAAGGTCAACACACCCACCAAAGTCCGGTGATGCCCCGAGGAAGCATCCGAGTCCTACCGGAATCCACCAACTGCCTCGTCGCTAATCACGACGCCGCCACCCCCGGTCTTCTATTCCAACACAACACTCCCCAGCCCTTCATGTACAGCCCTCCGCCGCCATTTCTAACGATCGACTCGTCGTCGGTGAGTCCCCATCCTCCGTCGTCCCTCCCACCGCGGTCGTCTCGAGATTCGTTGCTTCGAGACCATGGGCTTCTGCAGGACTTGGTGCCATTGCAAATGAGGAAGGAACCAAAAGATGAGCAAAATGGATGAATAATCAGAGGGAGAGTTTGAACTAATTTGAATATTATCTCTTTTTTGGTACGTTCAGAATTCAGATCAGCCATTTTCCATCGTTTTTTGAGCAGACGATTCTGATCTCATGATTATGAAACGCTTTCTCCTTAAGCGTTTTTTTTAGAGGGATGATTATATTAGTATTAGCTGTGCTTTATTAGTGAGTTTATTATCATTCTTTGTAACTAATTAATCTTAGCTAAAAACCAAGTTGTTTATTATGGGATATAATACGATCCAATCTTTTTTAGCTCA

mRNA sequence

ATGGAGAAGAAATTTGAAGACTCATTGATGGGTTCTGAAAATTCCGCCGGATTTTACTCTCCGGCCCTGTTCTCCGACGACTTTCCGGCCACTGGGTTTGGTTTTTCCGGTATGTTCGACATGCCATGTGACGATGATCATAAAGCTTCTTGTTCTTCGAATTCCTTTGGAATTGGAATCCACGATCTTTATAATCCTTCTTCTTTGTTCGATTTGCTTTCCACGGCGGCGCCGCCGCTGCAGCAGCCGCTTTCCTCGCCGGCCTCCACCGTGCCCGAGTCGTCCGAGGTGCTCAATGCTCCGGCCACGCCGAATTCTTCGTCGGTTTCGAATTCCTCCTCCAATGAAGCTGCGGCCATTGAAGAGGTCGACAAGAACAACAACACTGACAAGACTTCTAAAGTGTTGAAGCCAAAGAAGAAGAACCAGAAGAAGCAAAGAGAACCCAGATTTGCTTTCATGACAAAGAGTGATATCGATCATCTCGACGACGGTTACCGGTGGCGAAAGTACGGCCAAAAGGCCGTGAAGAACAGTCCTTATCCCAGAAGCTATTATCGTTGCACTACGGCAGGGTGTGGAGTGAAGAAGAGGGTGGAGCGGTCATCCGGCGACCATTCGATCGTCATCACAACGTACGAAGGTCAACACACCCACCAAAGTCCGGTGATGCCCCGAGGAAGCATCCGAGTCCTACCGGAATCCACCAACTGCCTCGTCGCTAATCACGACGCCGCCACCCCCGGTCTTCTATTCCAACACAACACTCCCCAGCCCTTCATGTACAGCCCTCCGCCGCCATTTCTAACGATCGACTCGTCGTCGGTGAGTCCCCATCCTCCGTCGTCCCTCCCACCGCGGTCGTCTCGAGATTCGTTGCTTCGAGACCATGGGCTTCTGCAGGACTTGGTGCCATTGCAAATGAGGAAGGAACCAAAAGATGAGCAAAATGGATGA

Coding sequence (CDS)

ATGGAGAAGAAATTTGAAGACTCATTGATGGGTTCTGAAAATTCCGCCGGATTTTACTCTCCGGCCCTGTTCTCCGACGACTTTCCGGCCACTGGGTTTGGTTTTTCCGGTATGTTCGACATGCCATGTGACGATGATCATAAAGCTTCTTGTTCTTCGAATTCCTTTGGAATTGGAATCCACGATCTTTATAATCCTTCTTCTTTGTTCGATTTGCTTTCCACGGCGGCGCCGCCGCTGCAGCAGCCGCTTTCCTCGCCGGCCTCCACCGTGCCCGAGTCGTCCGAGGTGCTCAATGCTCCGGCCACGCCGAATTCTTCGTCGGTTTCGAATTCCTCCTCCAATGAAGCTGCGGCCATTGAAGAGGTCGACAAGAACAACAACACTGACAAGACTTCTAAAGTGTTGAAGCCAAAGAAGAAGAACCAGAAGAAGCAAAGAGAACCCAGATTTGCTTTCATGACAAAGAGTGATATCGATCATCTCGACGACGGTTACCGGTGGCGAAAGTACGGCCAAAAGGCCGTGAAGAACAGTCCTTATCCCAGAAGCTATTATCGTTGCACTACGGCAGGGTGTGGAGTGAAGAAGAGGGTGGAGCGGTCATCCGGCGACCATTCGATCGTCATCACAACGTACGAAGGTCAACACACCCACCAAAGTCCGGTGATGCCCCGAGGAAGCATCCGAGTCCTACCGGAATCCACCAACTGCCTCGTCGCTAATCACGACGCCGCCACCCCCGGTCTTCTATTCCAACACAACACTCCCCAGCCCTTCATGTACAGCCCTCCGCCGCCATTTCTAACGATCGACTCGTCGTCGGTGAGTCCCCATCCTCCGTCGTCCCTCCCACCGCGGTCGTCTCGAGATTCGTTGCTTCGAGACCATGGGCTTCTGCAGGACTTGGTGCCATTGCAAATGAGGAAGGAACCAAAAGATGAGCAAAATGGATGA

Protein sequence

MEKKFEDSLMGSENSAGFYSPALFSDDFPATGFGFSGMFDMPCDDDHKASCSSNSFGIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSSNEAAAIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPESTNCLVANHDAATPGLLFQHNTPQPFMYSPPPPFLTIDSSSVSPHPPSSLPPRSSRDSLLRDHGLLQDLVPLQMRKEPKDEQNG
Homology
BLAST of Spg031450 vs. NCBI nr
Match: XP_022942903.1 (probable WRKY transcription factor 48 [Cucurbita moschata])

HSP 1 Score: 496.9 bits (1278), Expect = 1.3e-136
Identity = 268/328 (81.71%), Postives = 284/328 (86.59%), Query Frame = 0

Query: 1   MEKKFEDSLMGSENSAGFYSPALFSDDFPATGFGFSGMFDMPCDDDHKASCSSNSF---- 60
           MEKKFEDSLMGS  S G YS  +FSD+F   G GFSG+FD+PCDDDHKASCSS+S     
Sbjct: 1   MEKKFEDSLMGSATSTGIYSQTVFSDEFSVGGSGFSGVFDLPCDDDHKASCSSSSSFDLF 60

Query: 61  --GIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSS 120
             GIG HD YNPSS+FDLLST APPLQ PLSSP+STVPESSEVLNAP TPNSSSVSNSSS
Sbjct: 61  GGGIGFHDFYNPSSMFDLLSTTAPPLQPPLSSPSSTVPESSEVLNAPTTPNSSSVSNSSS 120

Query: 121 NEAAAIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQK 180
           NEAA IEEVDKN    KTSKVLKP KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQK
Sbjct: 121 NEAAPIEEVDKN---AKTSKVLKP-KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQK 180

Query: 181 AVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPE 240
           AVKNSPYPRSYYRCTT+ CGVKKRVERSS DHSIVITTYEGQHTHQSPVMPRGSIR+L E
Sbjct: 181 AVKNSPYPRSYYRCTTSQCGVKKRVERSSSDHSIVITTYEGQHTHQSPVMPRGSIRILSE 240

Query: 241 STNCLVANHDAATPGLLFQHNTP-QPFMYSPPPPFLTIDSSSVSPH-PPSSLPP--RSSR 300
           STNCLVA+HD AT G+LFQHN P QPFMY+PP PFLT DSS +SP  PPSSLPP   SSR
Sbjct: 241 STNCLVADHDVATTGILFQHNIPGQPFMYNPPQPFLTNDSSFLSPRPPPSSLPPPSLSSR 300

Query: 301 DSLLRDHGLLQDLVPLQMRKEPKDEQNG 319
            SLL+D+GLLQDLVPLQ+RKEPKDEQNG
Sbjct: 301 ASLLQDNGLLQDLVPLQVRKEPKDEQNG 324

BLAST of Spg031450 vs. NCBI nr
Match: XP_023540261.1 (probable WRKY transcription factor 48 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 489.2 bits (1258), Expect = 2.7e-134
Identity = 265/328 (80.79%), Postives = 284/328 (86.59%), Query Frame = 0

Query: 1   MEKKFEDSLMGSENSAGFYSPALFSDDFPATGFGFSGMFDMPCDDDHKAS-CSSNSF--- 60
           MEKKFEDSLMGS  S G YS  +FSD+FP  G GFSG+FD+PCDDDHKAS CSS+SF   
Sbjct: 1   MEKKFEDSLMGSATSTGIYSQTVFSDEFPVGGSGFSGVFDLPCDDDHKASCCSSSSFDLF 60

Query: 61  --GIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSS 120
             GIG HD YNPSS+FDLLST APPLQ PLSSP+STVPESSEVLNAP TPNSSSVSNSSS
Sbjct: 61  GGGIGFHDFYNPSSMFDLLSTTAPPLQPPLSSPSSTVPESSEVLNAPTTPNSSSVSNSSS 120

Query: 121 NEAAAIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQK 180
           NEA+ IEEVDKN    KTSK+LKP KKNQKKQRE RFAFMTKSDIDHLDDGYRWRKYGQK
Sbjct: 121 NEASPIEEVDKN----KTSKMLKP-KKNQKKQRERRFAFMTKSDIDHLDDGYRWRKYGQK 180

Query: 181 AVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPE 240
           AVKNSPYPRSYYRCTT+ CGVKKRVERSS DHSIVITTYEGQHTHQSPVMPRGSIR+L E
Sbjct: 181 AVKNSPYPRSYYRCTTSQCGVKKRVERSSSDHSIVITTYEGQHTHQSPVMPRGSIRILSE 240

Query: 241 STNCLVANHDAATPGLLFQHNTP-QPFMYSPPPPFLTIDSSSVSPH-PPSSLPP--RSSR 300
           STNCLVA+HD AT G+LFQHN P QPFMY+P  PFLT DSS +SP  PPSS+PP   SSR
Sbjct: 241 STNCLVADHDVATTGILFQHNVPGQPFMYNPLQPFLTNDSSFLSPRPPPSSVPPPSLSSR 300

Query: 301 DSLLRDHGLLQDLVPLQMRKEPKDEQNG 319
            SLL+D+GLLQDLVPLQ+RKEPKDEQNG
Sbjct: 301 ASLLQDNGLLQDLVPLQVRKEPKDEQNG 323

BLAST of Spg031450 vs. NCBI nr
Match: XP_022972028.1 (probable WRKY transcription factor 48 [Cucurbita maxima])

HSP 1 Score: 482.6 bits (1241), Expect = 2.5e-132
Identity = 259/327 (79.20%), Postives = 277/327 (84.71%), Query Frame = 0

Query: 1   MEKKFEDSLMGSENSAGFYSPALFSDDFPATGFGFSGMFDMPCDDDHKASCSSNSF---- 60
           MEKKFEDSLMGS  S G YS  +FSD+FP  G GFSG+FD+PCDD   + CSS+SF    
Sbjct: 1   MEKKFEDSLMGSATSTGIYSQTVFSDEFPVGGSGFSGVFDLPCDDHKASCCSSSSFDLFG 60

Query: 61  -GIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSSN 120
            GIG HD YNPSS+FDLLST  PPLQ PLSSP+STVPESSEVLNAP TPNSSSVSNSSSN
Sbjct: 61  GGIGFHDFYNPSSIFDLLSTTVPPLQPPLSSPSSTVPESSEVLNAPPTPNSSSVSNSSSN 120

Query: 121 EAAAIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKA 180
           EAA IEEVDKN    KTSKVLKP KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQK 
Sbjct: 121 EAAPIEEVDKN---AKTSKVLKP-KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKT 180

Query: 181 VKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPES 240
           VKNSPYPRSYYRCTT+ CGVKKRVERSS DHSIVITTYEGQHTHQSPVMPRGSIR+L ES
Sbjct: 181 VKNSPYPRSYYRCTTSQCGVKKRVERSSSDHSIVITTYEGQHTHQSPVMPRGSIRILSES 240

Query: 241 TNCLVANHDAATPGLLFQHNTP-QPFMYSPPPPFLTIDSSSVSPHPPS---SLPPRSSRD 300
           TNCLVA+HD AT G+LFQHN P QPFMY+PP PFLT +SS +SP PP    S P  SSR 
Sbjct: 241 TNCLVADHDVATTGILFQHNIPGQPFMYNPPQPFLTNESSFLSPRPPPPSLSPPLLSSRA 300

Query: 301 SLLRDHGLLQDLVPLQMRKEPKDEQNG 319
           SLL+D+GLLQDLVPLQ+RKEPKDEQNG
Sbjct: 301 SLLQDNGLLQDLVPLQVRKEPKDEQNG 323

BLAST of Spg031450 vs. NCBI nr
Match: KAG6597580.1 (putative WRKY transcription factor 48, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 472.6 bits (1215), Expect = 2.6e-129
Identity = 256/319 (80.25%), Postives = 273/319 (85.58%), Query Frame = 0

Query: 10  MGSENSAGFYSPALFSDDFPATGFGFSGMFDMPCDDDHKASCSSNSF------GIGIHDL 69
           MGS  S G YS  +FSD+FP  G GFSG+FD+PCDDDHKASCSS+S       GIG HD 
Sbjct: 1   MGSATSTGIYSQTVFSDEFPVGGSGFSGVFDLPCDDDHKASCSSSSSFDLFGGGIGFHDF 60

Query: 70  YNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSSNEAAAIEEV 129
           YNPSS+FDLLST A PLQ PLSSP+STVPESSEVLNAP TPNSSSVSNSSSNEAA IEEV
Sbjct: 61  YNPSSMFDLLSTTALPLQPPLSSPSSTVPESSEVLNAPTTPNSSSVSNSSSNEAAPIEEV 120

Query: 130 DKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPR 189
           D+N    KTSKVLKP KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPR
Sbjct: 121 DRN---AKTSKVLKP-KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPR 180

Query: 190 SYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPESTNCLVANH 249
           SYYRCTT+ CGVKKRVERSS DHSIVITTYEGQHTHQSPVMPRGSIR+L ESTNCLVA+H
Sbjct: 181 SYYRCTTSQCGVKKRVERSSSDHSIVITTYEGQHTHQSPVMPRGSIRILSESTNCLVADH 240

Query: 250 DAATPGLLFQHNTP-QPFMYSPPPPFLTIDSSSVSPH-PPSSLPP--RSSRDSLLRDHGL 309
           D  T G+LFQ N P QPFMY+PP PFLT DSS +SP  PPSSLPP   SSR SLL+D+GL
Sbjct: 241 DVVTTGILFQPNIPGQPFMYNPPQPFLTNDSSFLSPRPPPSSLPPPSLSSRASLLQDNGL 300

Query: 310 LQDLVPLQMRKEPKDEQNG 319
           LQDLVPLQ+RKEPKDEQNG
Sbjct: 301 LQDLVPLQVRKEPKDEQNG 315

BLAST of Spg031450 vs. NCBI nr
Match: KAG7029024.1 (putative WRKY transcription factor 48, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 470.3 bits (1209), Expect = 1.3e-128
Identity = 254/318 (79.87%), Postives = 271/318 (85.22%), Query Frame = 0

Query: 10  MGSENSAGFYSPALFSDDFPATGFGFSGMFDMPCDDDHKASCSSNSF------GIGIHDL 69
           MGS  S G YS  +FSD+FP  G GFSG+FD+PCDDDHKASCSS+S       GIG HD 
Sbjct: 1   MGSATSTGIYSQTVFSDEFPVGGSGFSGVFDLPCDDDHKASCSSSSSFDLFGGGIGFHDF 60

Query: 70  YNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSSNEAAAIEEV 129
           YNPSS+FDLLST  PPLQ PLSSP+STVPESSEVLNAP TPNSSSVSNSSSNEAA IEEV
Sbjct: 61  YNPSSMFDLLSTTTPPLQPPLSSPSSTVPESSEVLNAPTTPNSSSVSNSSSNEAAPIEEV 120

Query: 130 DKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPR 189
           D+N    KTSKVLKP KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPR
Sbjct: 121 DRN---AKTSKVLKP-KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPR 180

Query: 190 SYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPESTNCLVANH 249
           SYYRCTT+ CGVKKRVERSS DHSIVITTYEGQHTHQSPVMPRGSIR+L ESTNCLVA+H
Sbjct: 181 SYYRCTTSQCGVKKRVERSSSDHSIVITTYEGQHTHQSPVMPRGSIRILSESTNCLVADH 240

Query: 250 DAATPGLLFQHNTPQPFMYSPPPPFLTIDSSSVSPH-PPSSLPP--RSSRDSLLRDHGLL 309
           D  T G+LFQ    QPFMY+PP PFLT DSS +SP  PPSSLPP   SSR SLL+D+GLL
Sbjct: 241 DVVTTGILFQPG--QPFMYNPPQPFLTNDSSFLSPRPPPSSLPPPSLSSRASLLQDNGLL 300

Query: 310 QDLVPLQMRKEPKDEQNG 319
           QDLVPLQ+RKEPKDEQNG
Sbjct: 301 QDLVPLQVRKEPKDEQNG 312

BLAST of Spg031450 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 144/306 (47.06%), Postives = 173/306 (56.54%), Query Frame = 0

Query: 46  DHKASCSSNSFGIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLN-APATP 105
           DH+ + SSNS          P++  +       PL Q          ESSEV+N  P +P
Sbjct: 101 DHQFASSSNSSSFSFDAFPLPNNNNNTSFFTDLPLPQ---------AESSEVVNTTPTSP 160

Query: 106 NSSSVSNSSSNEAA------------AIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFA 165
           NS+SVS SSSNEAA              EE D+      T   LK KKKNQKK RE RFA
Sbjct: 161 NSTSVS-SSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQLKAKKKNQKKAREARFA 220

Query: 166 FMTKSDIDHLDDGYRWRKYGQKAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITT 225
           F+TKSDID+LDDGYRWRKYGQKAVKNSPYPRSYYRCTT GCGVKKRVERSS D SIV+TT
Sbjct: 221 FLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDDPSIVMTT 280

Query: 226 YEGQHTHQSPVMPRGSIRVLPESTNCLVANHDAATPG-----------LLFQHNTPQPFM 285
           YEGQHTH  P+ PRG I +L       + +H A T             LL QH+ P    
Sbjct: 281 YEGQHTHPFPMTPRGHIGMLTSP----ILDHGATTASSSSFSIPQPRYLLTQHHQPYNMY 340

Query: 286 YSPPPPFL---TIDSSSVSPHPPSSLP--------PRSSRDSLLRDHGLLQDLVPLQMRK 317
            +     +   + D + V+P P SS P          +S  S +RDHGLLQD++P Q+R 
Sbjct: 341 NNNSLSMINRRSSDGTFVNPGPSSSFPGFGYDMSQASTSTSSSIRDHGLLQDILPSQIRS 392

BLAST of Spg031450 vs. ExPASy Swiss-Prot
Match: O22900 (WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.5e-39
Identity = 118/271 (43.54%), Postives = 153/271 (56.46%), Query Frame = 0

Query: 66  PSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSS----NEAAAIE 125
           PS+        APP +Q ++S   ++     ++N PATPNSSS+S++SS     E    E
Sbjct: 68  PSAKLSSSIIQAPPSEQLVTSKVESLCSDHLLINPPATPNSSSISSASSEALNEEKPKTE 127

Query: 126 EVDKNNNTDK-----TSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAV 185
           + ++    D+     T K LK KK NQK+QRE R AFMTKS++DHL+DGYRWRKYGQKAV
Sbjct: 128 DNEEEGGEDQQEKSHTKKQLKAKKNNQKRQREARVAFMTKSEVDHLEDGYRWRKYGQKAV 187

Query: 186 KNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPR--------GS 245
           KNSP+PRSYYRCTTA C VKKRVERS  D S V+TTYEGQHTH SP+  R        GS
Sbjct: 188 KNSPFPRSYYRCTTASCNVKKRVERSFRDPSTVVTTYEGQHTHISPLTSRPISTGGFFGS 247

Query: 246 IRVLPESTN-CLVANHDAAT-------PGLLFQHNTPQPFMYSPPPPFLTIDSSSVSPHP 305
                   N C     D +T         + + H   Q  + S         +S  + + 
Sbjct: 248 SGAASSLGNGCFGFPIDGSTLISPQFQQLVQYHHQQQQQELMSCFGGVNEYLNSHANEYG 307

Query: 306 PSSLPPRSSRDSLLRDHGLLQDLVPLQMRKE 312
             +   +S    L++D+GLLQD+VP  M KE
Sbjct: 308 DDNRVKKSR--VLVKDNGLLQDVVPSHMLKE 336

BLAST of Spg031450 vs. ExPASy Swiss-Prot
Match: Q9C983 (Probable WRKY transcription factor 57 OS=Arabidopsis thaliana OX=3702 GN=WRKY57 PE=2 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 4.2e-37
Identity = 113/266 (42.48%), Postives = 147/266 (55.26%), Query Frame = 0

Query: 46  DHKASCSSNSFGIGI-HDLYNPSSL-FDLLSTAAPPLQQPLSSPASTVPESSEVLNAPAT 105
           D   S   + FG  + H   +P SL FD   T    ++   ++  S+   S+ V  A  +
Sbjct: 30  DRDTSNILSDFGWNLHHSSDHPHSLRFDSDLTQTTGVKP--TTVTSSCSSSAAVSVAVTS 89

Query: 106 PNSSSVSNSSSNEAAAIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLD 165
            N++  + SSS+E  A         T      +K KKK QK+ R+PRFAFMTKSD+D+L+
Sbjct: 90  TNNNPSATSSSSEDPAENSTASAEKTPPPETPVKEKKKAQKRIRQPRFAFMTKSDVDNLE 149

Query: 166 DGYRWRKYGQKAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPV 225
           DGYRWRKYGQKAVKNSP+PRSYYRCT + C VKKRVERSS D SIVITTYEGQH HQ+  
Sbjct: 150 DGYRWRKYGQKAVKNSPFPRSYYRCTNSRCTVKKRVERSSDDPSIVITTYEGQHCHQTIG 209

Query: 226 MPRGSIRVLPESTNCLVANHDAATPGLLFQHNTPQPFMYSPPPPFLTIDSSSVSPHPPSS 285
            PRG I +     +   ++H    P        P P+ Y      L  D+++ SP  P  
Sbjct: 210 FPRGGI-LTAHDPHSFTSHHHLPPP-------LPNPYYYQELLHQLHRDNNAPSPRLPRP 269

Query: 286 LPPRSSRDSLLRDHGLLQDLVPLQMR 310
               +   S   + GLL D+VP  MR
Sbjct: 270 TTEDTPAVSTPSEEGLLGDIVPQTMR 285

BLAST of Spg031450 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 6.3e-33
Identity = 111/244 (45.49%), Postives = 138/244 (56.56%), Query Frame = 0

Query: 83  PLSSPASTVPESSEVLNAPATPNSSSVSNSSSNEAAAIEEV-DKNNNTD----------K 142
           P SSP  +V  S        T NS  VS SSSNE    E   DK++  +          +
Sbjct: 44  PYSSPFQSVSPSVNNPYLNLTSNSPVVS-SSSNEGEPKENTNDKSDQMEDNEGDLHGVGE 103

Query: 143 TSKVL--KPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPRSYYRCT 202
           +SK L  + KKK +KK+RE R AFMTKS+IDHL+DGYRWRKYGQKAVKNSPYPRSYYRCT
Sbjct: 104 SSKQLTKQGKKKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCT 163

Query: 203 TAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPESTNCLVANHDAATPG 262
           T  C VKKRVERS  D SIVITTYEG+H H  P   RG++     + + LV  H      
Sbjct: 164 TQKCNVKKRVERSFQDPSIVITTYEGKHNHPIPSTLRGTV----AAEHLLV--HRGGGGS 223

Query: 263 LLF---QHNTPQPFMYSPPPPFLTIDSSSVSPHPPSSLPPRSSRDSLLRDHGLLQDLVPL 311
           LL    +H+     M   P  + ++ S S   H   +     + +  + D+GLLQD+VP 
Sbjct: 224 LLHSFPRHHQDFLMMKHSPANYQSVGSLSYE-HGHGTSSYNFNNNQPVVDYGLLQDIVPS 279

BLAST of Spg031450 vs. ExPASy Swiss-Prot
Match: Q8VWJ2 (WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 5.3e-32
Identity = 101/238 (42.44%), Postives = 129/238 (54.20%), Query Frame = 0

Query: 23  LFSDDFPATGFGFSGMFDMPCDDDHKASCSSNSFG-IGIHDLYNPS--SLFDLLSTAAPP 82
           L++  +P++ F    M ++P       + + +S+G +   + +NPS  S  D L ++   
Sbjct: 8   LYNYQYPSS-FSLHEMMNLP-------TSNPSSYGNLPSQNGFNPSTYSFTDCLQSSPAA 67

Query: 83  LQQPLSSPASTVPESSEVLNAP--------------------ATPNSSSVSNSSSNEAAA 142
            +  L       P SSEV N+                      T    S SNSSS+EA  
Sbjct: 68  YESLLQKTFGLSPSSSEVFNSSIDQEPNRDVTNDVINGGACNETETRVSPSNSSSSEADH 127

Query: 143 IEEVDKNNNTDK---------TSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRK 202
             E    +   +         + KV K KK   KKQREPR +FMTKS++DHL+DGYRWRK
Sbjct: 128 PGEDSGKSRRKRELVGEEDQISKKVGKTKKTEVKKQREPRVSFMTKSEVDHLEDGYRWRK 187

Query: 203 YGQKAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGS 229
           YGQKAVKNSPYPRSYYRCTT  C VKKRVERS  D ++VITTYEGQH H  P   RGS
Sbjct: 188 YGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPTVVITTYEGQHNHPIPTNLRGS 237

BLAST of Spg031450 vs. ExPASy TrEMBL
Match: A0A6J1FVX6 (probable WRKY transcription factor 48 OS=Cucurbita moschata OX=3662 GN=LOC111447790 PE=4 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 6.3e-137
Identity = 268/328 (81.71%), Postives = 284/328 (86.59%), Query Frame = 0

Query: 1   MEKKFEDSLMGSENSAGFYSPALFSDDFPATGFGFSGMFDMPCDDDHKASCSSNSF---- 60
           MEKKFEDSLMGS  S G YS  +FSD+F   G GFSG+FD+PCDDDHKASCSS+S     
Sbjct: 1   MEKKFEDSLMGSATSTGIYSQTVFSDEFSVGGSGFSGVFDLPCDDDHKASCSSSSSFDLF 60

Query: 61  --GIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSS 120
             GIG HD YNPSS+FDLLST APPLQ PLSSP+STVPESSEVLNAP TPNSSSVSNSSS
Sbjct: 61  GGGIGFHDFYNPSSMFDLLSTTAPPLQPPLSSPSSTVPESSEVLNAPTTPNSSSVSNSSS 120

Query: 121 NEAAAIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQK 180
           NEAA IEEVDKN    KTSKVLKP KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQK
Sbjct: 121 NEAAPIEEVDKN---AKTSKVLKP-KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQK 180

Query: 181 AVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPE 240
           AVKNSPYPRSYYRCTT+ CGVKKRVERSS DHSIVITTYEGQHTHQSPVMPRGSIR+L E
Sbjct: 181 AVKNSPYPRSYYRCTTSQCGVKKRVERSSSDHSIVITTYEGQHTHQSPVMPRGSIRILSE 240

Query: 241 STNCLVANHDAATPGLLFQHNTP-QPFMYSPPPPFLTIDSSSVSPH-PPSSLPP--RSSR 300
           STNCLVA+HD AT G+LFQHN P QPFMY+PP PFLT DSS +SP  PPSSLPP   SSR
Sbjct: 241 STNCLVADHDVATTGILFQHNIPGQPFMYNPPQPFLTNDSSFLSPRPPPSSLPPPSLSSR 300

Query: 301 DSLLRDHGLLQDLVPLQMRKEPKDEQNG 319
            SLL+D+GLLQDLVPLQ+RKEPKDEQNG
Sbjct: 301 ASLLQDNGLLQDLVPLQVRKEPKDEQNG 324

BLAST of Spg031450 vs. ExPASy TrEMBL
Match: A0A6J1I8P7 (probable WRKY transcription factor 48 OS=Cucurbita maxima OX=3661 GN=LOC111470683 PE=4 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 1.2e-132
Identity = 259/327 (79.20%), Postives = 277/327 (84.71%), Query Frame = 0

Query: 1   MEKKFEDSLMGSENSAGFYSPALFSDDFPATGFGFSGMFDMPCDDDHKASCSSNSF---- 60
           MEKKFEDSLMGS  S G YS  +FSD+FP  G GFSG+FD+PCDD   + CSS+SF    
Sbjct: 1   MEKKFEDSLMGSATSTGIYSQTVFSDEFPVGGSGFSGVFDLPCDDHKASCCSSSSFDLFG 60

Query: 61  -GIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSSN 120
            GIG HD YNPSS+FDLLST  PPLQ PLSSP+STVPESSEVLNAP TPNSSSVSNSSSN
Sbjct: 61  GGIGFHDFYNPSSIFDLLSTTVPPLQPPLSSPSSTVPESSEVLNAPPTPNSSSVSNSSSN 120

Query: 121 EAAAIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKA 180
           EAA IEEVDKN    KTSKVLKP KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQK 
Sbjct: 121 EAAPIEEVDKN---AKTSKVLKP-KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKT 180

Query: 181 VKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPES 240
           VKNSPYPRSYYRCTT+ CGVKKRVERSS DHSIVITTYEGQHTHQSPVMPRGSIR+L ES
Sbjct: 181 VKNSPYPRSYYRCTTSQCGVKKRVERSSSDHSIVITTYEGQHTHQSPVMPRGSIRILSES 240

Query: 241 TNCLVANHDAATPGLLFQHNTP-QPFMYSPPPPFLTIDSSSVSPHPPS---SLPPRSSRD 300
           TNCLVA+HD AT G+LFQHN P QPFMY+PP PFLT +SS +SP PP    S P  SSR 
Sbjct: 241 TNCLVADHDVATTGILFQHNIPGQPFMYNPPQPFLTNESSFLSPRPPPPSLSPPLLSSRA 300

Query: 301 SLLRDHGLLQDLVPLQMRKEPKDEQNG 319
           SLL+D+GLLQDLVPLQ+RKEPKDEQNG
Sbjct: 301 SLLQDNGLLQDLVPLQVRKEPKDEQNG 323

BLAST of Spg031450 vs. ExPASy TrEMBL
Match: A0A6J1IUK6 (probable WRKY transcription factor 48 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479433 PE=4 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 2.3e-118
Identity = 254/337 (75.37%), Postives = 275/337 (81.60%), Query Frame = 0

Query: 1   MEKKFEDSLMGSENSAGFYSPALFSDDFPATGFG-FSGMFDMPCDDDHK--ASCSSNSF- 60
           M+KK ED++MGSENSAG YSP +FSD+FPAT FG + G+FDMPC DDHK  ASCSSNSF 
Sbjct: 1   MDKKLEDTMMGSENSAGKYSPVVFSDEFPATSFGSYPGVFDMPC-DDHKAAASCSSNSFD 60

Query: 61  --GIGIHDLYNP-SSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSS 120
             G GI D YNP SSLFDL+ST AP     LSSPASTVPESSEV+NAP TPNSSSVS S 
Sbjct: 61  LLGSGI-DFYNPLSSLFDLVSTTAP----LLSSPASTVPESSEVVNAPPTPNSSSVS-SY 120

Query: 121 SNEAAAIEEVDKNNNTDK--TSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKY 180
           SN+AAAIEEVDK +NT+K  +S VLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKY
Sbjct: 121 SNDAAAIEEVDK-SNTEKPSSSNVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKY 180

Query: 181 GQKAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRV 240
           GQKAVKNSPYPRSYYRCTT GCGVKKRVER S D S V+TTYEGQHTHQSP++PRGSIRV
Sbjct: 181 GQKAVKNSPYPRSYYRCTTVGCGVKKRVERLSNDQSTVVTTYEGQHTHQSPIIPRGSIRV 240

Query: 241 LPESTNCLVANHD-AATPGLLFQHNTPQPFMYSPPPPFLTIDSSSVSPHPPSSL------ 300
           LPES      N D  +T GLLFQ NTPQ FMYSPPPPFLTI+S S +P PPSS       
Sbjct: 241 LPES------NLDVTSTTGLLFQPNTPQSFMYSPPPPFLTINSLSTNPRPPSSFPPPPPP 300

Query: 301 ---PPRSSRDSLLRDHGLLQDLVPLQMRKEPKDEQNG 319
              PP SS+  +LRDHGLLQDLVPLQMRKEPKDEQNG
Sbjct: 301 PPPPPPSSQAFMLRDHGLLQDLVPLQMRKEPKDEQNG 323

BLAST of Spg031450 vs. ExPASy TrEMBL
Match: A0A5D3CWI4 (Putative WRKY transcription factor 48 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001620 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.6e-116
Identity = 249/334 (74.55%), Postives = 273/334 (81.74%), Query Frame = 0

Query: 1   MEKKF-EDSLMGSENSAGFYSPALFSDDFPATGF-GFSGMFDMPCDDDHKASCSSNSF-- 60
           MEKKF E+S+MG ++SAG YSPA+FSDDFP++ F  FS +FDMPC D HKAS     +  
Sbjct: 1   MEKKFEEESMMGFDDSAGLYSPAVFSDDFPSSNFDSFSSIFDMPC-DAHKASNFDFYYYN 60

Query: 61  GIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSSNE 120
               ++  NPSS FDLLSTAAP    PLSSPASTVPESSEV+NAP TPNSSSVS SSSNE
Sbjct: 61  NSSNNNSDNPSSFFDLLSTAAP----PLSSPASTVPESSEVVNAPTTPNSSSVS-SSSNE 120

Query: 121 AAAIEEVDKNNNT-DK--TSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQ 180
           AAAIEEV+ +  T DK  +SKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQ
Sbjct: 121 AAAIEEVNNSTTTHDKPSSSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQ 180

Query: 181 KAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLP 240
           KAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDH+IV+TTYEGQHTHQSP+MPRGS+RVLP
Sbjct: 181 KAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHTIVVTTYEGQHTHQSPIMPRGSLRVLP 240

Query: 241 EST-NCLVANHDAATPGLLFQHNT-PQPFMY-SPPPPFLTIDSSSVS------PHPPSSL 300
           EST N L  +HD  T GLLFQHNT  QPFMY SPPPPFLTI+SSSV+      P PP S 
Sbjct: 241 ESTHNSLTVDHDTTTTGLLFQHNTSSQPFMYSSPPPPFLTINSSSVAATSTPHPPPPISF 300

Query: 301 PPRSSRDSLLRDHGLLQDLVPLQMRKEPKDEQNG 319
            P S   + +RDHGLLQDLVPLQMR EPKDEQNG
Sbjct: 301 QPPSLPQASVRDHGLLQDLVPLQMRNEPKDEQNG 328

BLAST of Spg031450 vs. ExPASy TrEMBL
Match: A0A0A0L834 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G168410 PE=4 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 1.4e-115
Identity = 248/330 (75.15%), Postives = 270/330 (81.82%), Query Frame = 0

Query: 1   MEKKF-EDSLMGSENSAGFYSPALFSDDFPATGF-GFSGMFDMPCDDDHKASCSSNSF-- 60
           MEKKF E+S MG ++SAGFYSPA+FSDDFP++ F  FS +FDMPC D HKAS     +  
Sbjct: 1   MEKKFEEESRMGFDDSAGFYSPAVFSDDFPSSNFDSFSSIFDMPC-DAHKASNFDFYYYN 60

Query: 61  GIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSSNE 120
               +   NPSS FDLLSTAAP    PLSSPASTVPESSEV+NAP TPNSSSVS SSSNE
Sbjct: 61  NNNSNSNNNPSSFFDLLSTAAP----PLSSPASTVPESSEVVNAPTTPNSSSVS-SSSNE 120

Query: 121 AAAIEEVDKNNNT-DK--TSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQ 180
           AAAIEEV+ +  T DK   SKVLKP KKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQ
Sbjct: 121 AAAIEEVNNSTTTHDKPSASKVLKPIKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQ 180

Query: 181 KAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLP 240
           KAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDH+IV+TTYEGQHTHQSP+MPRGS+RVLP
Sbjct: 181 KAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHTIVVTTYEGQHTHQSPIMPRGSLRVLP 240

Query: 241 EST-NCLVANHDAATPGLLFQHNTPQPFMY-SPPPPFLTIDSSSVSPH---PPSSLPPRS 300
           EST N L  +HD    GLLFQHNT QPFMY +PPPPFLTI+SSSV+     PP S  P S
Sbjct: 241 ESTNNSLTVDHDTTATGLLFQHNTSQPFMYIAPPPPFLTINSSSVAASHNPPPISFQPPS 300

Query: 301 SRDSLLRDHGLLQDLVPLQMRKEPKDEQNG 319
            + S +RDHGLLQDLVPLQMRKEPKDEQNG
Sbjct: 301 PQAS-VRDHGLLQDLVPLQMRKEPKDEQNG 323

BLAST of Spg031450 vs. TAIR 10
Match: AT5G49520.1 (WRKY DNA-binding protein 48 )

HSP 1 Score: 201.8 bits (512), Expect = 8.1e-52
Identity = 144/306 (47.06%), Postives = 173/306 (56.54%), Query Frame = 0

Query: 46  DHKASCSSNSFGIGIHDLYNPSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLN-APATP 105
           DH+ + SSNS          P++  +       PL Q          ESSEV+N  P +P
Sbjct: 101 DHQFASSSNSSSFSFDAFPLPNNNNNTSFFTDLPLPQ---------AESSEVVNTTPTSP 160

Query: 106 NSSSVSNSSSNEAA------------AIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFA 165
           NS+SVS SSSNEAA              EE D+      T   LK KKKNQKK RE RFA
Sbjct: 161 NSTSVS-SSSNEAANDNNSGKEVTVKDQEEGDQQQEQKGTKPQLKAKKKNQKKAREARFA 220

Query: 166 FMTKSDIDHLDDGYRWRKYGQKAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITT 225
           F+TKSDID+LDDGYRWRKYGQKAVKNSPYPRSYYRCTT GCGVKKRVERSS D SIV+TT
Sbjct: 221 FLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVERSSDDPSIVMTT 280

Query: 226 YEGQHTHQSPVMPRGSIRVLPESTNCLVANHDAATPG-----------LLFQHNTPQPFM 285
           YEGQHTH  P+ PRG I +L       + +H A T             LL QH+ P    
Sbjct: 281 YEGQHTHPFPMTPRGHIGMLTSP----ILDHGATTASSSSFSIPQPRYLLTQHHQPYNMY 340

Query: 286 YSPPPPFL---TIDSSSVSPHPPSSLP--------PRSSRDSLLRDHGLLQDLVPLQMRK 317
            +     +   + D + V+P P SS P          +S  S +RDHGLLQD++P Q+R 
Sbjct: 341 NNNSLSMINRRSSDGTFVNPGPSSSFPGFGYDMSQASTSTSSSIRDHGLLQDILPSQIRS 392

BLAST of Spg031450 vs. TAIR 10
Match: AT2G47260.1 (WRKY DNA-binding protein 23 )

HSP 1 Score: 164.9 bits (416), Expect = 1.1e-40
Identity = 118/271 (43.54%), Postives = 153/271 (56.46%), Query Frame = 0

Query: 66  PSSLFDLLSTAAPPLQQPLSSPASTVPESSEVLNAPATPNSSSVSNSSS----NEAAAIE 125
           PS+        APP +Q ++S   ++     ++N PATPNSSS+S++SS     E    E
Sbjct: 68  PSAKLSSSIIQAPPSEQLVTSKVESLCSDHLLINPPATPNSSSISSASSEALNEEKPKTE 127

Query: 126 EVDKNNNTDK-----TSKVLKPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAV 185
           + ++    D+     T K LK KK NQK+QRE R AFMTKS++DHL+DGYRWRKYGQKAV
Sbjct: 128 DNEEEGGEDQQEKSHTKKQLKAKKNNQKRQREARVAFMTKSEVDHLEDGYRWRKYGQKAV 187

Query: 186 KNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPR--------GS 245
           KNSP+PRSYYRCTTA C VKKRVERS  D S V+TTYEGQHTH SP+  R        GS
Sbjct: 188 KNSPFPRSYYRCTTASCNVKKRVERSFRDPSTVVTTYEGQHTHISPLTSRPISTGGFFGS 247

Query: 246 IRVLPESTN-CLVANHDAAT-------PGLLFQHNTPQPFMYSPPPPFLTIDSSSVSPHP 305
                   N C     D +T         + + H   Q  + S         +S  + + 
Sbjct: 248 SGAASSLGNGCFGFPIDGSTLISPQFQQLVQYHHQQQQQELMSCFGGVNEYLNSHANEYG 307

Query: 306 PSSLPPRSSRDSLLRDHGLLQDLVPLQMRKE 312
             +   +S    L++D+GLLQD+VP  M KE
Sbjct: 308 DDNRVKKSR--VLVKDNGLLQDVVPSHMLKE 336

BLAST of Spg031450 vs. TAIR 10
Match: AT1G69310.1 (WRKY DNA-binding protein 57 )

HSP 1 Score: 156.8 bits (395), Expect = 3.0e-38
Identity = 113/266 (42.48%), Postives = 147/266 (55.26%), Query Frame = 0

Query: 46  DHKASCSSNSFGIGI-HDLYNPSSL-FDLLSTAAPPLQQPLSSPASTVPESSEVLNAPAT 105
           D   S   + FG  + H   +P SL FD   T    ++   ++  S+   S+ V  A  +
Sbjct: 30  DRDTSNILSDFGWNLHHSSDHPHSLRFDSDLTQTTGVKP--TTVTSSCSSSAAVSVAVTS 89

Query: 106 PNSSSVSNSSSNEAAAIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLD 165
            N++  + SSS+E  A         T      +K KKK QK+ R+PRFAFMTKSD+D+L+
Sbjct: 90  TNNNPSATSSSSEDPAENSTASAEKTPPPETPVKEKKKAQKRIRQPRFAFMTKSDVDNLE 149

Query: 166 DGYRWRKYGQKAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPV 225
           DGYRWRKYGQKAVKNSP+PRSYYRCT + C VKKRVERSS D SIVITTYEGQH HQ+  
Sbjct: 150 DGYRWRKYGQKAVKNSPFPRSYYRCTNSRCTVKKRVERSSDDPSIVITTYEGQHCHQTIG 209

Query: 226 MPRGSIRVLPESTNCLVANHDAATPGLLFQHNTPQPFMYSPPPPFLTIDSSSVSPHPPSS 285
            PRG I +     +   ++H    P        P P+ Y      L  D+++ SP  P  
Sbjct: 210 FPRGGI-LTAHDPHSFTSHHHLPPP-------LPNPYYYQELLHQLHRDNNAPSPRLPRP 269

Query: 286 LPPRSSRDSLLRDHGLLQDLVPLQMR 310
               +   S   + GLL D+VP  MR
Sbjct: 270 TTEDTPAVSTPSEEGLLGDIVPQTMR 285

BLAST of Spg031450 vs. TAIR 10
Match: AT1G69310.2 (WRKY DNA-binding protein 57 )

HSP 1 Score: 156.8 bits (395), Expect = 3.0e-38
Identity = 113/266 (42.48%), Postives = 147/266 (55.26%), Query Frame = 0

Query: 46  DHKASCSSNSFGIGI-HDLYNPSSL-FDLLSTAAPPLQQPLSSPASTVPESSEVLNAPAT 105
           D   S   + FG  + H   +P SL FD   T    ++   ++  S+   S+ V  A  +
Sbjct: 30  DRDTSNILSDFGWNLHHSSDHPHSLRFDSDLTQTTGVKP--TTVTSSCSSSAAVSVAVTS 89

Query: 106 PNSSSVSNSSSNEAAAIEEVDKNNNTDKTSKVLKPKKKNQKKQREPRFAFMTKSDIDHLD 165
            N++  + SSS+E  A         T      +K KKK QK+ R+PRFAFMTKSD+D+L+
Sbjct: 90  TNNNPSATSSSSEDPAENSTASAEKTPPPETPVKEKKKAQKRIRQPRFAFMTKSDVDNLE 149

Query: 166 DGYRWRKYGQKAVKNSPYPRSYYRCTTAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPV 225
           DGYRWRKYGQKAVKNSP+PRSYYRCT + C VKKRVERSS D SIVITTYEGQH HQ+  
Sbjct: 150 DGYRWRKYGQKAVKNSPFPRSYYRCTNSRCTVKKRVERSSDDPSIVITTYEGQHCHQTIG 209

Query: 226 MPRGSIRVLPESTNCLVANHDAATPGLLFQHNTPQPFMYSPPPPFLTIDSSSVSPHPPSS 285
            PRG I +     +   ++H    P        P P+ Y      L  D+++ SP  P  
Sbjct: 210 FPRGGI-LTAHDPHSFTSHHHLPPP-------LPNPYYYQELLHQLHRDNNAPSPRLPRP 269

Query: 286 LPPRSSRDSLLRDHGLLQDLVPLQMR 310
               +   S   + GLL D+VP  MR
Sbjct: 270 TTEDTPAVSTPSEEGLLGDIVPQTMR 285

BLAST of Spg031450 vs. TAIR 10
Match: AT1G29860.1 (WRKY DNA-binding protein 71 )

HSP 1 Score: 142.9 bits (359), Expect = 4.4e-34
Identity = 111/244 (45.49%), Postives = 138/244 (56.56%), Query Frame = 0

Query: 83  PLSSPASTVPESSEVLNAPATPNSSSVSNSSSNEAAAIEEV-DKNNNTD----------K 142
           P SSP  +V  S        T NS  VS SSSNE    E   DK++  +          +
Sbjct: 44  PYSSPFQSVSPSVNNPYLNLTSNSPVVS-SSSNEGEPKENTNDKSDQMEDNEGDLHGVGE 103

Query: 143 TSKVL--KPKKKNQKKQREPRFAFMTKSDIDHLDDGYRWRKYGQKAVKNSPYPRSYYRCT 202
           +SK L  + KKK +KK+RE R AFMTKS+IDHL+DGYRWRKYGQKAVKNSPYPRSYYRCT
Sbjct: 104 SSKQLTKQGKKKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCT 163

Query: 203 TAGCGVKKRVERSSGDHSIVITTYEGQHTHQSPVMPRGSIRVLPESTNCLVANHDAATPG 262
           T  C VKKRVERS  D SIVITTYEG+H H  P   RG++     + + LV  H      
Sbjct: 164 TQKCNVKKRVERSFQDPSIVITTYEGKHNHPIPSTLRGTV----AAEHLLV--HRGGGGS 223

Query: 263 LLF---QHNTPQPFMYSPPPPFLTIDSSSVSPHPPSSLPPRSSRDSLLRDHGLLQDLVPL 311
           LL    +H+     M   P  + ++ S S   H   +     + +  + D+GLLQD+VP 
Sbjct: 224 LLHSFPRHHQDFLMMKHSPANYQSVGSLSYE-HGHGTSSYNFNNNQPVVDYGLLQDIVPS 279

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022942903.11.3e-13681.71probable WRKY transcription factor 48 [Cucurbita moschata][more]
XP_023540261.12.7e-13480.79probable WRKY transcription factor 48 [Cucurbita pepo subsp. pepo][more]
XP_022972028.12.5e-13279.20probable WRKY transcription factor 48 [Cucurbita maxima][more]
KAG6597580.12.6e-12980.25putative WRKY transcription factor 48, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7029024.11.3e-12879.87putative WRKY transcription factor 48, partial [Cucurbita argyrosperma subsp. ar... [more]
Match NameE-valueIdentityDescription
Q9FGZ41.1e-5047.06Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 ... [more]
O229001.5e-3943.54WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1[more]
Q9C9834.2e-3742.48Probable WRKY transcription factor 57 OS=Arabidopsis thaliana OX=3702 GN=WRKY57 ... [more]
Q93WV46.3e-3345.49WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1[more]
Q8VWJ25.3e-3242.44WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FVX66.3e-13781.71probable WRKY transcription factor 48 OS=Cucurbita moschata OX=3662 GN=LOC111447... [more]
A0A6J1I8P71.2e-13279.20probable WRKY transcription factor 48 OS=Cucurbita maxima OX=3661 GN=LOC11147068... [more]
A0A6J1IUK62.3e-11875.37probable WRKY transcription factor 48 isoform X1 OS=Cucurbita maxima OX=3661 GN=... [more]
A0A5D3CWI41.6e-11674.55Putative WRKY transcription factor 48 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A0A0L8341.4e-11575.15WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G168410 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT5G49520.18.1e-5247.06WRKY DNA-binding protein 48 [more]
AT2G47260.11.1e-4043.54WRKY DNA-binding protein 23 [more]
AT1G69310.13.0e-3842.48WRKY DNA-binding protein 57 [more]
AT1G69310.23.0e-3842.48WRKY DNA-binding protein 57 [more]
AT1G29860.14.4e-3445.49WRKY DNA-binding protein 71 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 162..221
e-value: 4.2E-39
score: 145.9
IPR003657WRKY domainPFAMPF03106WRKYcoord: 163..220
e-value: 9.0E-26
score: 89.9
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 157..222
score: 32.39732
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 146..223
e-value: 5.8E-34
score: 117.7
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 154..222
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 271..293
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..122
NoneNo IPR availablePANTHERPTHR31221:SF238WRKY TRANSCRIPTION FACTOR 48-RELATEDcoord: 80..271
IPR044810WRKY transcription factor, plantPANTHERPTHR31221WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATEDcoord: 80..271

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031450.1Spg031450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding