Spg031439 (gene) Sponge gourd (cylindrica) v1

Overview


Name	Spg031439
Type	gene
Organism	Luffa cylindrica (Sponge gourd (cylindrica) v1)
Description	Pectate lyase
Location	scaffold11: 41250075 .. 41254831 (-)
RNA-Seq Expression	Spg031439
Synteny	Spg031439

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATGGCGGAATCTGGAGGATGGATTCGGTTGTTTCTTCTCGTGGTGTTCATGTTGTTCTTCGTCGGAACAATTGCCAGAGTTCCCAAAGAGATTCCTGAAATTGAGTATGAATCTCTCTCTATCTCTCTAGAATTCGCGGAGTTTCTGTTTTGTTTATCTTAATTCTCATCTTCGTCGTCTGTCGGCGATTTTCCCTACTTTTCTGTTATTTGCTCTGTATTTACGGGATCCGAGTTCTCTCTGTTGTTCTCCCTGATTCGGTATGGATTTTAGCCACATTATGTGAATAGACGATCGATTTCGTTGCAATTTTAGAGTGTTGTTGTTTTCTAATTTCGTAGGCTCTTCAATTTATCGTCTGCGGTTGTGTTTTCTCAGTCCCATTGCATTCATGCTGTTTCACTCAGAGTTTTTCTTCTTTCTTTTCGCTTCTCATTTACGACTTCTGTCCTTTGAGCCGCTGAATTTACGTTATGGTTCGTCCTTGGAACCAATTTCGGTAATATAGTATTGTTATTGTCTTGTGTTGTACATTTCGACCTACCAAACATTTCAGTACGGAGACGGAGAGAATGCGAGGGAAGATGGAGAGTGCAATAAGCTCTTCCTTCAAACTTTAATTTCTCTGTTTTTCTAAACTTGTGAGTTCAATAAATTTTGCAGGAATGGGGCAACTGAGGTGTTGCAGAGCTCAAGCAACTCATCAATGGCAGTGAGGTGAATTATTTTTTCTCTTTTCTTCTCAATGAATAAGTTCAGTTGAAAGAATAAATGCGGCAGAATCAGTACCTAACCAAACTCCTTCGGACGTTAATACTTAATACCCTCCCACCTTCCCCTATTCTTAACTGATCTCAGCCGTCTGTTTTTTGGCCTTCTTTCTCTGTTTATTTACACTATTGGGCTTTTAACTTGTACTATTTTTCTCCCAACTCTTGTGCCCATCTCCATCGTCTAGATCTAGATCCCTGGATCTGAACCGTTTATTTCGTTACCCGTTACTTCTTAGGATTCCTCTCCTCCAGTAGCAAATTTGGGCGTTCCTCCCAGATTTGCAACCTTTTCTCTTTTATTATGTTATTTGGAGTGACAAAAAGATTATGACATTTTCATTTGTGAGATTGTTTGTGAAAATGGACTTTTCCCGCCGTTCTCTATTCTATATTCGCTCTTAGAAAAGGCCCTTTTGACGGTTTTTTCGTAGCGAGCGAGCTGTTCGAGTATCTTTCCACCTTGAGTGATGCACAAAATTCACTGTATTGGACACTGTAGTACGTGATGTGATAAGAAAAAACGCGTTACACCACTCGCTTTTTTGCACTCGCTTTGTGTGTTTGCCACGTTTTGCTTTATGATTTGCTGCCTACTGGAGTTTCCCCAAATTGCATGTGCCTCTGTCCTGGTCACAATGCATTATTGTCCCTGCCAAAAATCGATATCGTGCCTATCAACTGTCGGTTCTAAATTATTATTCGTTTCTTCTTTTCTTTTGGGGTCAATTTTTTTTCTTATTGGATCTGATAAATGGGGATGGTAATTTCAGGTTGGACGAGGTTGAGGCTCCCGGTAATAAGCATGCCGTTGACAACCCAGAAGAGATCGCTGCAATGGTTGACCTGTGGGTCCTCTATTCCCCTTCGTATAGATTATAAAATTGTGAAATAGTACTATGTATAGTCTAGAATTTGAATTATCTGTAGGGAAACTATCTGGGTGTGGATTAGGCTCAACGGTCCTGAAGCAGGTCTTCTGGGATCAAATAAGGAAAATTAAGTAAACCTGTCCATCTGACCAAATTTCACTACCCGACCTTAAAAAGATCGCTAATAATATGCACATGGCCACTTTTTTGCCCTTCATTGCCAGTTTTTCGGTAAATTGAATCTTTGACTCCAGAGTTGAAATCCCCCATTTTATCTTTCGTCTCTCCTTAATTAACGACGCCTTAGATTTACAACTAGGTGTCTGTCCATAGCCCTGTCTATTTCATTCTGTTTTTGCCTTTTGGTCTTATTTCGGAACAGGACAGTTCAGTGTGGATATGTTTATTTTCTAGGATCTTGTTATCCAGTCCATGTGGACTTTGTAAAATGAGGCAAATGAAATTTAAGTTGATTTGAACGAGTTTATTGCAAGTTGCAACTGCCCTTGAGTGGCCCAATTGACGACAAGGACCCGAAAAAAATGCCACCAAACTTTTACCCATCAAACTGAAGAGTCTTCCACAAATTGACAAATGGGATTGCTTTATTCTATGGCTCCCATCATCCCGTTCTTGTTTTGGTCTAAATTTAATTATTTAAGAAATTTACCCCACCGACGCTGCACGCAAGAGTTTCAGCGGTTATTTCTTTCTATAATGTGCTTGTTATAGTTGCTTAATTCTGTGAACGTTTTATAATATCGAAGGTTAACAGTTAAACAATATACTTGATCAGGAGTATCCGCAATGCCACTGCGAGGAGAGAGCTTGGTTTCTTCTCATGTGGAACCGGCAATCCTATTGATGACTGCTGGCGTTGCGACCCCAATTGGCAACAGAACCGCAAACGTCTGGCAGATTGTGGCATTGGCTTTGGCCGAAATGCCATCGGCGGTCGCGATGGTCGATTCTACGTCATCACTGATTCTGGTGATGACGACCCTGTCAACCCAAGGCCAGGCACTTTGCGCCATGCCGTCATCCAGGATGAACCTCTCTGGATTGTGTTCAAAAGAGACATGGTGATACAGCTCAAACAGGAGCTGATCATGAATAGTTTCAAGACAATTGATGCTCGTGGGACCAATGTTCACATTGCTAATGGTGCTTGCCTCACAATCCAGTTTGTGACCAATGTTATTGTCCATGGTCTTAACATCCATGACTGCAAGCCCACTGGAAACGCCATGGTAAGGAGCTCTCCCAATCACGTTGGGTGGAGAACAATGGCTGATGGGGATGCCATTTCCATTTTCGGGTCAAGTCATATTTGGATCGATCACAATTCACTCTCTAATTGTGCTGATGGCCTTGTCGATGCTGTCATGGGATCCACTGCAATTACCATCACCAACAATTACTTCACCCACCACAATGAGGTATCTTCACTTCATCCATCTACTCAGTTCGTATTTTTGCCATTTTTCAGAGACATTGCATCACGTTGTGTGTTTAATGAACAGGTAATGTTATTGGGACATAGTGATTCCTACGTTAGAGATAAACAAATGCAAGTGACCATTGCTTACAATCACTTTGGAGAAGGACTCATCCAAAGAATGCCAAGGTACAATCATTCATCATTATTGTCTTTTATTCATTTCTAGCAAGCACGGTTTTATGAAATGTCAGCTTCATGCTGCAATGTCTTGATGAGAGACCAAGACCATAGATAGATATTGGCTTAAATCTCTAGACTGCTAAACGACAATGTTTGGTCACTTATAAGCAAAAGAACAATAACAACATCCGAACCCGTTTCTAATCTTACAAAATGGAATCAATTTATCCACTATGTAGGTGTAGGCACGGATACTTCCATGTGGTGAACAATGATTATACTCACTGGGAGATGTACGCCATTGGTGGAAGTGCTAATCCAACCATCAACAGCCAGGGTAACAGATATGCTGCTCCGACCAACCCTTTTGCAAAAGAGGTACTAAATAATACCGCATCACCAATTAGTTTAAACTTTTAAACTTTTAAGTTCAGCAATAATTTAACATGATATTCGAGTAAGAACTATGATCTAGTTTATCAGATGCTGATGCAACTAATCTTCGTCGACATTCGTTAGGTGACCAAGAGGGTTGAGACAGCTGAATCTCAGTGGAAAAACTGGAACTGGAGATCAGAGGGAGACCTATTGTTGAATGGTGCCTTTTTCACTCCATCTGGTGCAGGGGCCTCGGCAAGCTATGCCAGAGCCTCAAGTTTGGGAGCTAAATCCTCATCCATGGTTGCTTCCATAACATCCAGCGCCGGTGCGCTTTCCTGCCGGAGAGGCCATCCCTGCTAGCATTCCGATCCCGTCGCCGATCACAGAACACCCCCTCTATCATTTTTCTGTGAATTTTTCAAATATTTACTTACAAATAGCCTTACCCCAATATCCATATTCTATCAATTGATATCTCCTTCCAACTCCTTCTCTCTCTCTCTCTCTATCATATCTAAAAACAAGTGTACATTTTCTCTCGTCTCATTACAGAGACGTTTCCTATTCGACTCGTCCCAGTTTGCTGTTCGTTGAGACACTTCGAAATTGCTCGACCTCGGCCTGCTTCAACTTGAAAAAGACACCATTTGCCATTCAGCAAGCAGGTGCGGAAGCGTTGTTCTTGTGTTCATTTATATTCCCTTCTCAGTTGGCCACTTTTCTTCTTCCCAATCCATTCCTCGTCTTGCTTCAGCTATCATTGTTACTCTCTATGTGTACTGTTCTGTCTTCAGGTTCTTATAGGATTTGAATGATTGGGTGTTTTTTTTTTCCTTTTTTTGTATTTTTTTTTCCTTTTTCTTCTTTTACACTTTGTTCTGTTTAGTGATACAAGCTATAGTATGTTTGTCAAACATTGAATTATCAAAGCAAAAGTTTGCTAAAGATGATAGCAAATATTGTTGATAATATGCCCTTGTCTATCTTTCACCTACTTTTTCCCTTTTTTAATTGTTCTTGGAGGTAGAGTTGTGATGTAGGGTCGAGAAGTGATGTGAAATAAATTCATTTAGTTGAATTTTAAAACCCATATATTTTTCTTTCACC

mRNA sequence

ATGGCAATGGCGGAATCTGGAGGATGGATTCGGTTGTTTCTTCTCGTGGTGTTCATGTTGTTCTTCGTCGGAACAATTGCCAGAGTTCCCAAAGAGATTCCTGAAATTGAGTATGAATCTCTCTCTATCTCTCTAGAATTCGCGGAGTTTCTGTTTTCCGCTGAATTTACGTTATGGAATGGGGCAACTGAGGTGTTGCAGAGCTCAAGCAACTCATCAATGGCAGTGAGGTTGGACGAGGTTGAGGCTCCCGGTAATAAGCATGCCGTTGACAACCCAGAAGAGATCGCTGCAATGGTTGACCTGAGTATCCGCAATGCCACTGCGAGGAGAGAGCTTGGTTTCTTCTCATGTGGAACCGGCAATCCTATTGATGACTGCTGGCGTTGCGACCCCAATTGGCAACAGAACCGCAAACGTCTGGCAGATTGTGGCATTGGCTTTGGCCGAAATGCCATCGGCGGTCGCGATGGTCGATTCTACGTCATCACTGATTCTGGTGATGACGACCCTGTCAACCCAAGGCCAGGCACTTTGCGCCATGCCGTCATCCAGGATGAACCTCTCTGGATTGTGTTCAAAAGAGACATGGTGATACAGCTCAAACAGGAGCTGATCATGAATAGTTTCAAGACAATTGATGCTCGTGGGACCAATGTTCACATTGCTAATGGTGCTTGCCTCACAATCCAGTTTGTGACCAATGTTATTGTCCATGGTCTTAACATCCATGACTGCAAGCCCACTGGAAACGCCATGGTAAGGAGCTCTCCCAATCACGTTGGGTGGAGAACAATGGCTGATGGGGATGCCATTTCCATTTTCGGGTCAAGTCATATTTGGATCGATCACAATTCACTCTCTAATTGTGCTGATGGCCTTGTCGATGCTGTCATGGGATCCACTGCAATTACCATCACCAACAATTACTTCACCCACCACAATGAGGTATCTTCACTTCATCCATCTACTCAGTTCGTATTTTTGCCATTTTTCAGAGACATTGCATCACGTTGTGTGTTTAATGAACAGGTAATGTTATTGGGACATAGTGATTCCTACGTTAGAGATAAACAAATGCAAGTGACCATTGCTTACAATCACTTTGGAGAAGGACTCATCCAAAGAATGCCAAGGTGTAGGCACGGATACTTCCATGTGGTGAACAATGATTATACTCACTGGGAGATGTACGCCATTGGTGGAAGTGCTAATCCAACCATCAACAGCCAGGGTAACAGATATGCTGCTCCGACCAACCCTTTTGCAAAAGAGGTGACCAAGAGGGTTGAGACAGCTGAATCTCAGTGGAAAAACTGGAACTGGAGATCAGAGGGAGACCTATTGTTGAATGGTGCCTTTTTCACTCCATCTGGTGCAGGGGCCTCGGCAAGCTATGCCAGAGCCTCAAGTTTGGGAGCTAAATCCTCATCCATGGTTGCTTCCATAACATCCAGCGCCGGTGCGCTTTCCTGCCGGAGAGGCCATCCCTGCTAG

Coding sequence (CDS)

ATGGCAATGGCGGAATCTGGAGGATGGATTCGGTTGTTTCTTCTCGTGGTGTTCATGTTGTTCTTCGTCGGAACAATTGCCAGAGTTCCCAAAGAGATTCCTGAAATTGAGTATGAATCTCTCTCTATCTCTCTAGAATTCGCGGAGTTTCTGTTTTCCGCTGAATTTACGTTATGGAATGGGGCAACTGAGGTGTTGCAGAGCTCAAGCAACTCATCAATGGCAGTGAGGTTGGACGAGGTTGAGGCTCCCGGTAATAAGCATGCCGTTGACAACCCAGAAGAGATCGCTGCAATGGTTGACCTGAGTATCCGCAATGCCACTGCGAGGAGAGAGCTTGGTTTCTTCTCATGTGGAACCGGCAATCCTATTGATGACTGCTGGCGTTGCGACCCCAATTGGCAACAGAACCGCAAACGTCTGGCAGATTGTGGCATTGGCTTTGGCCGAAATGCCATCGGCGGTCGCGATGGTCGATTCTACGTCATCACTGATTCTGGTGATGACGACCCTGTCAACCCAAGGCCAGGCACTTTGCGCCATGCCGTCATCCAGGATGAACCTCTCTGGATTGTGTTCAAAAGAGACATGGTGATACAGCTCAAACAGGAGCTGATCATGAATAGTTTCAAGACAATTGATGCTCGTGGGACCAATGTTCACATTGCTAATGGTGCTTGCCTCACAATCCAGTTTGTGACCAATGTTATTGTCCATGGTCTTAACATCCATGACTGCAAGCCCACTGGAAACGCCATGGTAAGGAGCTCTCCCAATCACGTTGGGTGGAGAACAATGGCTGATGGGGATGCCATTTCCATTTTCGGGTCAAGTCATATTTGGATCGATCACAATTCACTCTCTAATTGTGCTGATGGCCTTGTCGATGCTGTCATGGGATCCACTGCAATTACCATCACCAACAATTACTTCACCCACCACAATGAGGTATCTTCACTTCATCCATCTACTCAGTTCGTATTTTTGCCATTTTTCAGAGACATTGCATCACGTTGTGTGTTTAATGAACAGGTAATGTTATTGGGACATAGTGATTCCTACGTTAGAGATAAACAAATGCAAGTGACCATTGCTTACAATCACTTTGGAGAAGGACTCATCCAAAGAATGCCAAGGTGTAGGCACGGATACTTCCATGTGGTGAACAATGATTATACTCACTGGGAGATGTACGCCATTGGTGGAAGTGCTAATCCAACCATCAACAGCCAGGGTAACAGATATGCTGCTCCGACCAACCCTTTTGCAAAAGAGGTGACCAAGAGGGTTGAGACAGCTGAATCTCAGTGGAAAAACTGGAACTGGAGATCAGAGGGAGACCTATTGTTGAATGGTGCCTTTTTCACTCCATCTGGTGCAGGGGCCTCGGCAAGCTATGCCAGAGCCTCAAGTTTGGGAGCTAAATCCTCATCCATGGTTGCTTCCATAACATCCAGCGCCGGTGCGCTTTCCTGCCGGAGAGGCCATCCCTGCTAG

Protein sequence

MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWNGATEVLQSSSNSSMAVRLDEVEAPGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC

Homology

[Close]

BLAST of Spg031439 vs. NCBI nr
Match: XP_022924851.1 (probable pectate lyase 8 [Cucurbita moschata] >KAG6582448.1 putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018839.1 putative pectate lyase 15 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 850.5 bits (2196), Expect = 7.3e-243
Identity = 430/500 (86.00%), Postives = 441/500 (88.20%), Query Frame = 0


Query: 1   MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWN 60
           MAMAESGGWIRLFLLVVFML FVG IARVPKEIPEI                       N
Sbjct: 1   MAMAESGGWIRLFLLVVFML-FVGAIARVPKEIPEIH----------------------N 60

Query: 61  GATEVLQSSSNSSMAVRLDEVEA--PGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSC 120
            AT  LQSSSNSSMAVRL+E EA  PGNKHAVDNP+EIAAMVD+SIRNATARRELGFFSC
Sbjct: 61  EATGKLQSSSNSSMAVRLEEPEAETPGNKHAVDNPDEIAAMVDMSIRNATARRELGFFSC 120

Query: 121 GTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGT 180
           GTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGT
Sbjct: 121 GTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGT 180

Query: 181 LRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIV 240
           LRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARG+NVHIANGAC+TIQFVTNVIV
Sbjct: 181 LRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGSNVHIANGACITIQFVTNVIV 240

Query: 241 HGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAV 300
           HGLNIHDCKPTGNAMVRSSPNHVGWRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAV
Sbjct: 241 HGLNIHDCKPTGNAMVRSSPNHVGWRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAV 300

Query: 301 MGSTAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDK 360
           MGSTAITITNNYFTHHNE                            VMLLGHSDSYVRDK
Sbjct: 301 MGSTAITITNNYFTHHNE----------------------------VMLLGHSDSYVRDK 360

Query: 361 QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP 420
           QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP
Sbjct: 361 QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP 420

Query: 421 TNPFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSS 480
           TNPFAKEVTKRVETAES+WKNWNWRSEGDLL+NGAFFTPSGAGASASYARASSLGAKSSS
Sbjct: 421 TNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSS 449

Query: 481 MVASITSSAGALSCRRGHPC 499
           MVA+IT++AGALSCRRGHPC
Sbjct: 481 MVATITATAGALSCRRGHPC 449

[Close]

BLAST of Spg031439 vs. NCBI nr
Match: XP_023527818.1 (probable pectate lyase 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 846.7 bits (2186), Expect = 1.0e-241
Identity = 426/500 (85.20%), Postives = 440/500 (88.00%), Query Frame = 0


Query: 1   MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWN 60
           M MAESGGWIRLFLLVVFML FVG IAR+PK+IPEI+                      N
Sbjct: 1   MTMAESGGWIRLFLLVVFML-FVGAIARIPKDIPEID----------------------N 60

Query: 61  GATEVLQSSSNSSMAVRLDEVEA--PGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSC 120
            AT  LQSSSNSSMAVRL+E EA  PGNKHAVDNP+EIAAMVD+SIRNAT RRELGFFSC
Sbjct: 61  EATGKLQSSSNSSMAVRLEEPEAETPGNKHAVDNPDEIAAMVDMSIRNATTRRELGFFSC 120

Query: 121 GTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGT 180
           GTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGT
Sbjct: 121 GTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGT 180

Query: 181 LRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIV 240
           LRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARG+NVHIANGAC+TIQFVTNVIV
Sbjct: 181 LRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGSNVHIANGACITIQFVTNVIV 240

Query: 241 HGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAV 300
           HGLNIHDCKPTGNAMVRSSPNHVGWRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAV
Sbjct: 241 HGLNIHDCKPTGNAMVRSSPNHVGWRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAV 300

Query: 301 MGSTAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDK 360
           MGSTAITITNNYFTHHNE                            VMLLGHSDSYVRDK
Sbjct: 301 MGSTAITITNNYFTHHNE----------------------------VMLLGHSDSYVRDK 360

Query: 361 QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP 420
           QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP
Sbjct: 361 QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP 420

Query: 421 TNPFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSS 480
           TNPFAKEVTKRVETAES+WKNWNWRSEGDLL+NGAFFTPSGAGASASYARASSLGAKSSS
Sbjct: 421 TNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSS 449

Query: 481 MVASITSSAGALSCRRGHPC 499
           MVA+IT++AGALSCRRGHPC
Sbjct: 481 MVATITATAGALSCRRGHPC 449

[Close]

BLAST of Spg031439 vs. NCBI nr
Match: XP_022974867.1 (probable pectate lyase 8 [Cucurbita maxima])

HSP 1 Score: 845.9 bits (2184), Expect = 1.8e-241
Identity = 423/498 (84.94%), Postives = 441/498 (88.55%), Query Frame = 0


Query: 1   MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWN 60
           MAMAESGGWIRLFLLVVFML FV TIARVPKEIPEIE                      N
Sbjct: 1   MAMAESGGWIRLFLLVVFML-FVETIARVPKEIPEIE----------------------N 60

Query: 61  GATEVLQSSSNSSMAVRLDEVEAPGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGT 120
            ATE+L+SSSN+SMA RL++VEA GNKHAVDNPEE+AA VD+SIRNATARRELGFFSCGT
Sbjct: 61  RATEILRSSSNASMAERLEDVEALGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGT 120

Query: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLR 180
           GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGR+YV+TDSG+DDP++PRPGTLR
Sbjct: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRYYVVTDSGNDDPISPRPGTLR 180

Query: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHG 240
           HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGAC+TIQFVTNVIVHG
Sbjct: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHG 240

Query: 241 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300
           LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG
Sbjct: 241 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300

Query: 301 STAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQM 360
           STAITITNNYFTHHNE                            VMLLGHSDSYVRDKQM
Sbjct: 301 STAITITNNYFTHHNE----------------------------VMLLGHSDSYVRDKQM 360

Query: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420
           QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN
Sbjct: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420

Query: 421 PFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 480
           PFAKEVTKRVETAES+WKNWNWRSEGDLL+NGAFFTPSGAGASASYARASSLGAKSSSMV
Sbjct: 421 PFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSSMV 447

Query: 481 ASITSSAGALSCRRGHPC 499
           ASIT++AGAL+CRRGH C
Sbjct: 481 ASITANAGALNCRRGHAC 447

[Close]

BLAST of Spg031439 vs. NCBI nr
Match: KAG6597311.1 (putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 844.3 bits (2180), Expect = 5.2e-241
Identity = 423/498 (84.94%), Postives = 441/498 (88.55%), Query Frame = 0


Query: 1   MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWN 60
           MAMAESGGWIRLFLLVVFML FV TIARVPKEIPEIE                      N
Sbjct: 1   MAMAESGGWIRLFLLVVFML-FVETIARVPKEIPEIE----------------------N 60

Query: 61  GATEVLQSSSNSSMAVRLDEVEAPGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGT 120
            ATE+L+SSSN+SMA RL++VEA GNKHAVDNPEE+AA VD+SIRNATARRELGFFSCGT
Sbjct: 61  RATEILRSSSNASMAERLEDVEALGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGT 120

Query: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLR 180
           GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGR+YV+TDSG+D+PV+PRPGTLR
Sbjct: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRYYVVTDSGNDNPVSPRPGTLR 180

Query: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHG 240
           HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGAC+TIQFVTNVIVHG
Sbjct: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHG 240

Query: 241 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300
           LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG
Sbjct: 241 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300

Query: 301 STAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQM 360
           STAITITNNYFTHHNE                            VMLLGHSDSYVRDKQM
Sbjct: 301 STAITITNNYFTHHNE----------------------------VMLLGHSDSYVRDKQM 360

Query: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420
           QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN
Sbjct: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420

Query: 421 PFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 480
           PFAKEVTKRVETAES+WKNWNWRSEGDLL+NGAFFTPSGAGASASYARASSLGAKSSSMV
Sbjct: 421 PFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSSMV 447

Query: 481 ASITSSAGALSCRRGHPC 499
           ASIT++AGAL+CRRGH C
Sbjct: 481 ASITANAGALNCRRGHAC 447

[Close]

BLAST of Spg031439 vs. NCBI nr
Match: XP_038895013.1 (probable pectate lyase 8 [Benincasa hispida])

HSP 1 Score: 844.0 bits (2179), Expect = 6.8e-241
Identity = 423/498 (84.94%), Postives = 437/498 (87.75%), Query Frame = 0


Query: 1   MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWN 60
           M MAESGGW RLFLLVVFML FVGTIARVPKEIPEI+                      N
Sbjct: 1   MTMAESGGWTRLFLLVVFML-FVGTIARVPKEIPEIQ----------------------N 60

Query: 61  GATEVLQSSSNSSMAVRLDEVEAPGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGT 120
           GATE ++SSSNSSMAVRLDE EA GNKHAVDNPEEIAAMVD+SIRNATARRELGFFSCGT
Sbjct: 61  GATEKMRSSSNSSMAVRLDEAEALGNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGT 120

Query: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLR 180
           GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFY++TDSGDD+PVNP+PGTLR
Sbjct: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYMVTDSGDDNPVNPKPGTLR 180

Query: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHG 240
           HAVIQDEPLWIVFKRDMVI LKQELIMNSFKTIDARGTNVHIANGAC+TIQFVTNVIVHG
Sbjct: 181 HAVIQDEPLWIVFKRDMVIHLKQELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHG 240

Query: 241 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300
           LNIHDCKPTGNAMVRSSPNHVGWRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG
Sbjct: 241 LNIHDCKPTGNAMVRSSPNHVGWRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300

Query: 301 STAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQM 360
           STAITI+NNYFTHHNE                            VMLLGHSDSYVRDK M
Sbjct: 301 STAITISNNYFTHHNE----------------------------VMLLGHSDSYVRDKLM 360

Query: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420
           QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN
Sbjct: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420

Query: 421 PFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 480
           PFAKEVTKRVETAE++WKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV
Sbjct: 421 PFAKEVTKRVETAETEWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 447

Query: 481 ASITSSAGALSCRRGHPC 499
            +ITS AGALSCRRGH C
Sbjct: 481 GTITSDAGALSCRRGHRC 447

[Close]

BLAST of Spg031439 vs. ExPASy Swiss-Prot
Match: Q944R1 (Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 2.0e-195
Identity = 334/441 (75.74%), Postives = 371/441 (84.13%), Query Frame = 0


Query: 63  TEVLQSSSNSSMAVRLDEVEAPGNKHAV-DNP----EEIAAMVDLSIRNATARRELGFFS 122
           TE  QS ++S+MA    + E    +HAV D+P    +E+A +V +S +N TARR+LGFFS
Sbjct: 58  TEEFQSLNSSTMAATRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFS 117

Query: 123 CGTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPG 182
           CGTGNPIDDCWRCD NW +NRKRLADCGIGFGRNAIGGRDGRFY++TD  D+D VNP+PG
Sbjct: 118 CGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPG 177

Query: 183 TLRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVI 242
           TLRHAVIQ+EPLWIVFKRDMVI+LKQELIMNSFKTIDARG+NVHIANGAC+TIQF+TNVI
Sbjct: 178 TLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVI 237

Query: 243 VHGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDA 302
           +HGL+IHDCKPTGNAMVRSSP+H GWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDA
Sbjct: 238 IHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDA 297

Query: 303 VMGSTAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRD 362
           VMGSTAIT++NN+FTHHNE                            VMLLGHSDSY +D
Sbjct: 298 VMGSTAITVSNNHFTHHNE----------------------------VMLLGHSDSYTKD 357

Query: 363 KQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAA 422
           K MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRYAA
Sbjct: 358 KLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAA 417

Query: 423 PTNPFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSS 482
           P + FAKEVTKRVET  S+WK WNWRSEGDLLLNGAFF PSGAGASASY RASSL AK S
Sbjct: 418 PMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAKPS 470

Query: 483 SMVASITSSAGALSCRRGHPC 499
           SMV +ITS+AGAL CR+G PC
Sbjct: 478 SMVDTITSTAGALGCRKGRPC 470

[Close]

BLAST of Spg031439 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 680.6 bits (1755), Expect = 1.3e-194
Identity = 334/432 (77.31%), Postives = 358/432 (82.87%), Query Frame = 0


Query: 71  NSSMAVRLDEVEAPGNKHAVDNPEEIA----AMVDLSIRNATARRELGFFSCGTGNPIDD 130
           N S   R DE E   N+HAV NP+E+A    A+ ++S+RN T RR+LG+F+CGTGNPIDD
Sbjct: 28  NISSLPRSDETE--WNQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDD 87

Query: 131 CWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQD 190
           CWRCDPNW +NRKRLADCGIGFGRNAIGGRDGRFYV+TD  DD+PVNPRPGTLRHAVIQD
Sbjct: 88  CWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQD 147

Query: 191 EPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDC 250
            PLWIVFKRDMVIQLKQELI+NSFKTID RG NVHIANG C+TIQFVTNVIVHGL+IHDC
Sbjct: 148 RPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDC 207

Query: 251 KPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI 310
           KPTGNAMVRSS  H GWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITI
Sbjct: 208 KPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITI 267

Query: 311 TNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQMQVTIAY 370
           +NN+ THHNE                            VMLLGHSDSY+RDK MQVTIAY
Sbjct: 268 SNNHLTHHNE----------------------------VMLLGHSDSYMRDKAMQVTIAY 327

Query: 371 NHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEV 430
           NHFG GLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP NPFAKEV
Sbjct: 328 NHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPKNPFAKEV 387

Query: 431 TKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMVASITSS 490
           TKRV+T  S WK WNWRSEGDLL NGA+FT SGA AS SYARASSL AKSSS+V  ITS 
Sbjct: 388 TKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGSYARASSLSAKSSSLVGHITSD 429

Query: 491 AGALSCRRGHPC 499
           AGAL CRRG  C
Sbjct: 448 AGALPCRRGRQC 429

[Close]

BLAST of Spg031439 vs. ExPASy Swiss-Prot
Match: Q9M8Z8 (Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 7.2e-193
Identity = 323/413 (78.21%), Postives = 353/413 (85.47%), Query Frame = 0


Query: 86  NKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 145
           ++HAV+NP+E+AAMVD+SIRN+T RR LG+FSC TGNPIDDCWRCD  WQ  RKRLADC 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 146 IGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 205
           IGFGRNAIGGRDGRFYV+TD GDDDPVNP PGTLRHAVIQDEPLWI+FKRDMVI LKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 206 IMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 265
           IMNSFKTID RG NVHIANGACLTIQ+VTN+IVHG+++HDCKPTGNAMVRSSP+H G+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 266 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVSSLHPSTQ 325
           MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT++NN+FTHHNE         
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNE--------- 271

Query: 326 FVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF 385
                              VMLLGHSDSY RDK MQVTIAYNHFGEGLIQRMPRCRHGYF
Sbjct: 272 -------------------VMLLGHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYF 331

Query: 386 HVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESQWKNWNWRSE 445
           HVVNNDYTHWEMYAIGGSA PTINSQGNR+ AP NPFAKEVTKR  T ES+WK+WNWRSE
Sbjct: 332 HVVNNDYTHWEMYAIGGSAGPTINSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSE 391

Query: 446 GDLLLNGAFFTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC 499
           GDL LNGAFFT SGAGA A+YARASSL AKSSS+V ++TS +GAL+CR G  C
Sbjct: 392 GDLFLNGAFFTRSGAGAGANYARASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

[Close]

BLAST of Spg031439 vs. ExPASy Swiss-Prot
Match: Q93WF1 (Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.1e-188
Identity = 315/413 (76.27%), Postives = 348/413 (84.26%), Query Frame = 0


Query: 86  NKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 145
           ++HAV+NPEE+AAMVD+SIRN+TARR LG+FSC TGNPIDDCWRCD  WQ  RK LA+C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 146 IGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 205
           IGFGRNAIGGRDGR+YV++D  DD+PVNP+PGTLRHAVIQ+EPLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 206 IMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 265
           IMNSFKTID RG NVHIANGAC+TIQFVTN+I+HG++IHDC+PTGNAMVRSSP+H GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 266 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVSSLHPSTQ 325
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITI+NNYFTHHNE         
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNE--------- 272

Query: 326 FVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF 385
                              VMLLGHSD+Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYF
Sbjct: 273 -------------------VMLLGHSDTYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYF 332

Query: 386 HVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESQWKNWNWRSE 445
           HVVNNDYTHWEMYAIGGSA+PTINSQGNRY AP N FAKEVTKR    + QW++WNWRSE
Sbjct: 333 HVVNNDYTHWEMYAIGGSASPTINSQGNRYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSE 392

Query: 446 GDLLLNGAFFTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC 499
           GDL LNGAFFT SG+G  ASYARASSL AKSSS+V  IT +AGAL+CR G  C
Sbjct: 393 GDLFLNGAFFTRSGSGLGASYARASSLAAKSSSLVGVITYNAGALNCRGGRRC 417

[Close]

BLAST of Spg031439 vs. ExPASy Swiss-Prot
Match: Q9LJ42 (Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 658.7 bits (1698), Expect = 5.3e-188
Identity = 318/431 (73.78%), Postives = 352/431 (81.67%), Query Frame = 0


Query: 68  SSSNSSMAVRLDEVEAPGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGTGNPIDDC 127
           SSSNSS A +L   +   N+HAV NPEE+AAMVD+ I+N+T RR LGFFSC TGNPIDDC
Sbjct: 38  SSSNSSTANKLPNDDGAWNEHAVKNPEEVAAMVDMKIKNSTERRRLGFFSCATGNPIDDC 97

Query: 128 WRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQDE 187
           WRCD NW   RKRLA+C IGFGRNAIGGRDGR+YV+TD  D D VNPRPGTLRHAVIQD 
Sbjct: 98  WRCDRNWHLRRKRLANCAIGFGRNAIGGRDGRYYVVTDPSDHDAVNPRPGTLRHAVIQDR 157

Query: 188 PLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCK 247
           PLWIVFKRDMVI L QELIMNSFKTID RG NV IA GAC+TIQ+VTN+I+HG+N+HDC+
Sbjct: 158 PLWIVFKRDMVITLTQELIMNSFKTIDGRGVNVAIAGGACITIQYVTNIIIHGINVHDCR 217

Query: 248 PTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITIT 307
            TGNAMVRSSP+H GWRTMADGDAISIFGSSHIWIDHNSLSNCADGL+DA+MGSTAITI+
Sbjct: 218 RTGNAMVRSSPSHYGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLIDAIMGSTAITIS 277

Query: 308 NNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQMQVTIAYN 367
           NNY THHNE                            VML+GHSDSY RDK MQVTIAYN
Sbjct: 278 NNYMTHHNE----------------------------VMLMGHSDSYTRDKLMQVTIAYN 337

Query: 368 HFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVT 427
           HFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSANPTINSQGNR+ AP NPFAKEVT
Sbjct: 338 HFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFAKEVT 397

Query: 428 KRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMVASITSSA 487
           KRV + + +WK WNWRS+GDL+LNGA+FT SGA A ASYARASSLGAK +S+V+ +T S+
Sbjct: 398 KRVGSWQGEWKQWNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPASVVSMLTYSS 440

Query: 488 GALSCRRGHPC 499
           GAL CR G  C
Sbjct: 458 GALKCRIGMRC 440

[Close]

BLAST of Spg031439 vs. ExPASy TrEMBL
Match: A0A6J1EAL4 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111432214 PE=3 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 3.5e-243
Identity = 430/500 (86.00%), Postives = 441/500 (88.20%), Query Frame = 0


Query: 1   MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWN 60
           MAMAESGGWIRLFLLVVFML FVG IARVPKEIPEI                       N
Sbjct: 1   MAMAESGGWIRLFLLVVFML-FVGAIARVPKEIPEIH----------------------N 60

Query: 61  GATEVLQSSSNSSMAVRLDEVEA--PGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSC 120
            AT  LQSSSNSSMAVRL+E EA  PGNKHAVDNP+EIAAMVD+SIRNATARRELGFFSC
Sbjct: 61  EATGKLQSSSNSSMAVRLEEPEAETPGNKHAVDNPDEIAAMVDMSIRNATARRELGFFSC 120

Query: 121 GTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGT 180
           GTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGT
Sbjct: 121 GTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGT 180

Query: 181 LRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIV 240
           LRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARG+NVHIANGAC+TIQFVTNVIV
Sbjct: 181 LRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGSNVHIANGACITIQFVTNVIV 240

Query: 241 HGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAV 300
           HGLNIHDCKPTGNAMVRSSPNHVGWRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAV
Sbjct: 241 HGLNIHDCKPTGNAMVRSSPNHVGWRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAV 300

Query: 301 MGSTAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDK 360
           MGSTAITITNNYFTHHNE                            VMLLGHSDSYVRDK
Sbjct: 301 MGSTAITITNNYFTHHNE----------------------------VMLLGHSDSYVRDK 360

Query: 361 QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP 420
           QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP
Sbjct: 361 QMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP 420

Query: 421 TNPFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSS 480
           TNPFAKEVTKRVETAES+WKNWNWRSEGDLL+NGAFFTPSGAGASASYARASSLGAKSSS
Sbjct: 421 TNPFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSS 449

Query: 481 MVASITSSAGALSCRRGHPC 499
           MVA+IT++AGALSCRRGHPC
Sbjct: 481 MVATITATAGALSCRRGHPC 449

[Close]

BLAST of Spg031439 vs. ExPASy TrEMBL
Match: A0A6J1IBF4 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111473637 PE=3 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 8.7e-242
Identity = 423/498 (84.94%), Postives = 441/498 (88.55%), Query Frame = 0


Query: 1   MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWN 60
           MAMAESGGWIRLFLLVVFML FV TIARVPKEIPEIE                      N
Sbjct: 1   MAMAESGGWIRLFLLVVFML-FVETIARVPKEIPEIE----------------------N 60

Query: 61  GATEVLQSSSNSSMAVRLDEVEAPGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGT 120
            ATE+L+SSSN+SMA RL++VEA GNKHAVDNPEE+AA VD+SIRNATARRELGFFSCGT
Sbjct: 61  RATEILRSSSNASMAERLEDVEALGNKHAVDNPEEVAAAVDMSIRNATARRELGFFSCGT 120

Query: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLR 180
           GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGR+YV+TDSG+DDP++PRPGTLR
Sbjct: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRYYVVTDSGNDDPISPRPGTLR 180

Query: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHG 240
           HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGAC+TIQFVTNVIVHG
Sbjct: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACITIQFVTNVIVHG 240

Query: 241 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300
           LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG
Sbjct: 241 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300

Query: 301 STAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQM 360
           STAITITNNYFTHHNE                            VMLLGHSDSYVRDKQM
Sbjct: 301 STAITITNNYFTHHNE----------------------------VMLLGHSDSYVRDKQM 360

Query: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420
           QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN
Sbjct: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420

Query: 421 PFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 480
           PFAKEVTKRVETAES+WKNWNWRSEGDLL+NGAFFTPSGAGASASYARASSLGAKSSSMV
Sbjct: 421 PFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSSMV 447

Query: 481 ASITSSAGALSCRRGHPC 499
           ASIT++AGAL+CRRGH C
Sbjct: 481 ASITANAGALNCRRGHAC 447

[Close]

BLAST of Spg031439 vs. ExPASy TrEMBL
Match: A0A6J1CZC3 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111016150 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 4.3e-241
Identity = 426/498 (85.54%), Postives = 438/498 (87.95%), Query Frame = 0


Query: 1   MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWN 60
           +AMA SG WIRL LLVVFML FVGTIARVPKEI + +                      N
Sbjct: 66  VAMAASGKWIRLVLLVVFML-FVGTIARVPKEISQTQ----------------------N 125

Query: 61  GATEVLQSSSNSSMAVRLDEVEAPGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGT 120
           GATE+LQSSSNSSMAVRLDEVEA  NKHAVDNPEEIAAMVD+SIRNATARRELGFFSCGT
Sbjct: 126 GATEMLQSSSNSSMAVRLDEVEALDNKHAVDNPEEIAAMVDMSIRNATARRELGFFSCGT 185

Query: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLR 180
           GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYV+TDSGDD+PVNP PGTLR
Sbjct: 186 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPMPGTLR 245

Query: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHG 240
           HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARG NVHIANGAC+TIQFVTNVIVHG
Sbjct: 246 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGANVHIANGACITIQFVTNVIVHG 305

Query: 241 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300
           LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG
Sbjct: 306 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 365

Query: 301 STAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQM 360
           STAITIT+NYFTHHNE                            VMLLGHSDSYVRDKQM
Sbjct: 366 STAITITSNYFTHHNE----------------------------VMLLGHSDSYVRDKQM 425

Query: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420
           QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN
Sbjct: 426 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 485

Query: 421 PFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 480
           PFAKEVTKRVETAE++WK+WNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV
Sbjct: 486 PFAKEVTKRVETAETEWKSWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 512

Query: 481 ASITSSAGALSCRRGHPC 499
           ASITSSAGALSCRRGHPC
Sbjct: 546 ASITSSAGALSCRRGHPC 512

[Close]

BLAST of Spg031439 vs. ExPASy TrEMBL
Match: A0A6J1IVV8 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111479711 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 9.6e-241
Identity = 425/498 (85.34%), Postives = 439/498 (88.15%), Query Frame = 0


Query: 3   MAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWNGA 62
           MAESGGWIRLFLLVVF+L FVG IARVPK+IPEI+                      N A
Sbjct: 1   MAESGGWIRLFLLVVFLL-FVGAIARVPKDIPEID----------------------NEA 60

Query: 63  TEVLQSSSNSSMAVRLDEVEA--PGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGT 122
           T  LQSSSNSSMAVRL+E EA  P NKHAVDNP+EIAAMVD+SIRNATARRELGFFSCGT
Sbjct: 61  TGKLQSSSNSSMAVRLEEPEAETPVNKHAVDNPDEIAAMVDMSIRNATARRELGFFSCGT 120

Query: 123 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLR 182
           GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLR 180

Query: 183 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHG 242
           HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARG+NVHIANGAC+TIQFVTNVIVHG
Sbjct: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGSNVHIANGACITIQFVTNVIVHG 240

Query: 243 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 302
           LNIHDCKPTGNAMVRSSPNHVGWRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG
Sbjct: 241 LNIHDCKPTGNAMVRSSPNHVGWRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300

Query: 303 STAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQM 362
           STAITITNNYFTHHNE                            VMLLGHSDSYVRDKQM
Sbjct: 301 STAITITNNYFTHHNE----------------------------VMLLGHSDSYVRDKQM 360

Query: 363 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 422
           QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN
Sbjct: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420

Query: 423 PFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 482
           PFAKEVTKRVETAES+WKNWNWRSEGDLL+NGAFFTPSGAGASASYARASSLGAKSSSMV
Sbjct: 421 PFAKEVTKRVETAESEWKNWNWRSEGDLLMNGAFFTPSGAGASASYARASSLGAKSSSMV 447

Query: 483 ASITSSAGALSCRRGHPC 499
           A+IT++AGALSCRRGHPC
Sbjct: 481 ATITATAGALSCRRGHPC 447

[Close]

BLAST of Spg031439 vs. ExPASy TrEMBL
Match: A0A5D3D285 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002440 PE=3 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 3.1e-239
Identity = 421/498 (84.54%), Postives = 435/498 (87.35%), Query Frame = 0


Query: 1   MAMAESGGWIRLFLLVVFMLFFVGTIARVPKEIPEIEYESLSISLEFAEFLFSAEFTLWN 60
           MAMAESG WIRL LLV+FML FVG IARVPKEIPE E                      N
Sbjct: 1   MAMAESGKWIRLILLVMFML-FVGAIARVPKEIPETE----------------------N 60

Query: 61  GATEVLQSSSNSSMAVRLDEVEAPGNKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGT 120
           G +E LQSSSNS+MAVRLDE +  GN+HAVDNPEEIAAMVD+SIRNATARRELGFFSCGT
Sbjct: 61  GGSEKLQSSSNSTMAVRLDETKDLGNEHAVDNPEEIAAMVDMSIRNATARRELGFFSCGT 120

Query: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLR 180
           GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYV+TDSGDD+PVNP+PGTLR
Sbjct: 121 GNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVVTDSGDDNPVNPKPGTLR 180

Query: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHG 240
           HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHG
Sbjct: 181 HAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHG 240

Query: 241 LNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300
           LNIHDCKPTGNAMVRSSPNHVGWRT+ADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG
Sbjct: 241 LNIHDCKPTGNAMVRSSPNHVGWRTIADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMG 300

Query: 301 STAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQM 360
           STAITI+NNYFTHHNE                            VMLLGHSDSYVRDK M
Sbjct: 301 STAITISNNYFTHHNE----------------------------VMLLGHSDSYVRDKLM 360

Query: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420
           QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN
Sbjct: 361 QVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTN 420

Query: 421 PFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 480
           PFAKEVTKRVETAES+WK WNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV
Sbjct: 421 PFAKEVTKRVETAESEWKGWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMV 447

Query: 481 ASITSSAGALSCRRGHPC 499
            +ITS+AGALSCRRGHPC
Sbjct: 481 GTITSNAGALSCRRGHPC 447

[Close]

BLAST of Spg031439 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.4e-196
Identity = 334/441 (75.74%), Postives = 371/441 (84.13%), Query Frame = 0


Query: 63  TEVLQSSSNSSMAVRLDEVEAPGNKHAV-DNP----EEIAAMVDLSIRNATARRELGFFS 122
           TE  QS ++S+MA    + E    +HAV D+P    +E+A +V +S +N TARR+LGFFS
Sbjct: 58  TEEFQSLNSSTMAATRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFS 117

Query: 123 CGTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPG 182
           CGTGNPIDDCWRCD NW +NRKRLADCGIGFGRNAIGGRDGRFY++TD  D+D VNP+PG
Sbjct: 118 CGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPG 177

Query: 183 TLRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVI 242
           TLRHAVIQ+EPLWIVFKRDMVI+LKQELIMNSFKTIDARG+NVHIANGAC+TIQF+TNVI
Sbjct: 178 TLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVI 237

Query: 243 VHGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDA 302
           +HGL+IHDCKPTGNAMVRSSP+H GWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDA
Sbjct: 238 IHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDA 297

Query: 303 VMGSTAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRD 362
           VMGSTAIT++NN+FTHHNE                            VMLLGHSDSY +D
Sbjct: 298 VMGSTAITVSNNHFTHHNE----------------------------VMLLGHSDSYTKD 357

Query: 363 KQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAA 422
           K MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRYAA
Sbjct: 358 KLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAA 417

Query: 423 PTNPFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSS 482
           P + FAKEVTKRVET  S+WK WNWRSEGDLLLNGAFF PSGAGASASY RASSL AK S
Sbjct: 418 PMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAKPS 470

Query: 483 SMVASITSSAGALSCRRGHPC 499
           SMV +ITS+AGAL CR+G PC
Sbjct: 478 SMVDTITSTAGALGCRKGRPC 470

[Close]

BLAST of Spg031439 vs. TAIR 10
Match: AT4G13710.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.4e-196
Identity = 334/441 (75.74%), Postives = 371/441 (84.13%), Query Frame = 0


Query: 63  TEVLQSSSNSSMAVRLDEVEAPGNKHAV-DNP----EEIAAMVDLSIRNATARRELGFFS 122
           TE  QS ++S+MA    + E    +HAV D+P    +E+A +V +S +N TARR+LGFFS
Sbjct: 39  TEEFQSLNSSTMAATRLDGEPQQQQHAVADDPDMVADEVAKLVQMSEQNRTARRKLGFFS 98

Query: 123 CGTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPG 182
           CGTGNPIDDCWRCD NW +NRKRLADCGIGFGRNAIGGRDGRFY++TD  D+D VNP+PG
Sbjct: 99  CGTGNPIDDCWRCDRNWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPG 158

Query: 183 TLRHAVIQDEPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVI 242
           TLRHAVIQ+EPLWIVFKRDMVI+LKQELIMNSFKTIDARG+NVHIANGAC+TIQF+TNVI
Sbjct: 159 TLRHAVIQEEPLWIVFKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVI 218

Query: 243 VHGLNIHDCKPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDA 302
           +HGL+IHDCKPTGNAMVRSSP+H GWRTMADGDA+SIFGSSHIWIDHNSLS+CADGLVDA
Sbjct: 219 IHGLHIHDCKPTGNAMVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDA 278

Query: 303 VMGSTAITITNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRD 362
           VMGSTAIT++NN+FTHHNE                            VMLLGHSDSY +D
Sbjct: 279 VMGSTAITVSNNHFTHHNE----------------------------VMLLGHSDSYTKD 338

Query: 363 KQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAA 422
           K MQVTIAYNHFGEGL+QRMPRCRHGYFHVVNNDYTHWEMYAIGGSA PTINSQGNRYAA
Sbjct: 339 KLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYAA 398

Query: 423 PTNPFAKEVTKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSS 482
           P + FAKEVTKRVET  S+WK WNWRSEGDLLLNGAFF PSGAGASASY RASSL AK S
Sbjct: 399 PMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSGAGASASYGRASSLAAKPS 451

Query: 483 SMVASITSSAGALSCRRGHPC 499
           SMV +ITS+AGAL CR+G PC
Sbjct: 459 SMVDTITSTAGALGCRKGRPC 451

[Close]

BLAST of Spg031439 vs. TAIR 10
Match: AT1G04680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 680.6 bits (1755), Expect = 9.3e-196
Identity = 334/432 (77.31%), Postives = 358/432 (82.87%), Query Frame = 0


Query: 71  NSSMAVRLDEVEAPGNKHAVDNPEEIA----AMVDLSIRNATARRELGFFSCGTGNPIDD 130
           N S   R DE E   N+HAV NP+E+A    A+ ++S+RN T RR+LG+F+CGTGNPIDD
Sbjct: 28  NISSLPRSDETE--WNQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDD 87

Query: 131 CWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQD 190
           CWRCDPNW +NRKRLADCGIGFGRNAIGGRDGRFYV+TD  DD+PVNPRPGTLRHAVIQD
Sbjct: 88  CWRCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQD 147

Query: 191 EPLWIVFKRDMVIQLKQELIMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDC 250
            PLWIVFKRDMVIQLKQELI+NSFKTID RG NVHIANG C+TIQFVTNVIVHGL+IHDC
Sbjct: 148 RPLWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDC 207

Query: 251 KPTGNAMVRSSPNHVGWRTMADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITI 310
           KPTGNAMVRSS  H GWRTMADGDAISIFGSSH+WIDHNSLS+CADGLVDAVMGSTAITI
Sbjct: 208 KPTGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITI 267

Query: 311 TNNYFTHHNEVSSLHPSTQFVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQMQVTIAY 370
           +NN+ THHNE                            VMLLGHSDSY+RDK MQVTIAY
Sbjct: 268 SNNHLTHHNE----------------------------VMLLGHSDSYMRDKAMQVTIAY 327

Query: 371 NHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEV 430
           NHFG GLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAP NPFAKEV
Sbjct: 328 NHFGVGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPKNPFAKEV 387

Query: 431 TKRVETAESQWKNWNWRSEGDLLLNGAFFTPSGAGASASYARASSLGAKSSSMVASITSS 490
           TKRV+T  S WK WNWRSEGDLL NGA+FT SGA AS SYARASSL AKSSS+V  ITS 
Sbjct: 388 TKRVDTPASHWKGWNWRSEGDLLQNGAYFTSSGAAASGSYARASSLSAKSSSLVGHITSD 429

Query: 491 AGALSCRRGHPC 499
           AGAL CRRG  C
Sbjct: 448 AGALPCRRGRQC 429

[Close]

BLAST of Spg031439 vs. TAIR 10
Match: AT3G07010.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 674.9 bits (1740), Expect = 5.1e-194
Identity = 323/413 (78.21%), Postives = 353/413 (85.47%), Query Frame = 0


Query: 86  NKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 145
           ++HAV+NP+E+AAMVD+SIRN+T RR LG+FSC TGNPIDDCWRCD  WQ  RKRLADC 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 146 IGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 205
           IGFGRNAIGGRDGRFYV+TD GDDDPVNP PGTLRHAVIQDEPLWI+FKRDMVI LKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 206 IMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 265
           IMNSFKTID RG NVHIANGACLTIQ+VTN+IVHG+++HDCKPTGNAMVRSSP+H G+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 266 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVSSLHPSTQ 325
           MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVM STAIT++NN+FTHHNE         
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNE--------- 271

Query: 326 FVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF 385
                              VMLLGHSDSY RDK MQVTIAYNHFGEGLIQRMPRCRHGYF
Sbjct: 272 -------------------VMLLGHSDSYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYF 331

Query: 386 HVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESQWKNWNWRSE 445
           HVVNNDYTHWEMYAIGGSA PTINSQGNR+ AP NPFAKEVTKR  T ES+WK+WNWRSE
Sbjct: 332 HVVNNDYTHWEMYAIGGSAGPTINSQGNRFLAPVNPFAKEVTKREYTGESKWKHWNWRSE 391

Query: 446 GDLLLNGAFFTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC 499
           GDL LNGAFFT SGAGA A+YARASSL AKSSS+V ++TS +GAL+CR G  C
Sbjct: 392 GDLFLNGAFFTRSGAGAGANYARASSLSAKSSSLVGTMTSYSGALNCRAGRRC 416

[Close]

BLAST of Spg031439 vs. TAIR 10
Match: AT5G48900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 661.0 bits (1704), Expect = 7.6e-190
Identity = 315/413 (76.27%), Postives = 348/413 (84.26%), Query Frame = 0


Query: 86  NKHAVDNPEEIAAMVDLSIRNATARRELGFFSCGTGNPIDDCWRCDPNWQQNRKRLADCG 145
           ++HAV+NPEE+AAMVD+SIRN+TARR LG+FSC TGNPIDDCWRCD  WQ  RK LA+C 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 146 IGFGRNAIGGRDGRFYVITDSGDDDPVNPRPGTLRHAVIQDEPLWIVFKRDMVIQLKQEL 205
           IGFGRNAIGGRDGR+YV++D  DD+PVNP+PGTLRHAVIQ+EPLWIVFKRDMVI LK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 206 IMNSFKTIDARGTNVHIANGACLTIQFVTNVIVHGLNIHDCKPTGNAMVRSSPNHVGWRT 265
           IMNSFKTID RG NVHIANGAC+TIQFVTN+I+HG++IHDC+PTGNAMVRSSP+H GWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 266 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMGSTAITITNNYFTHHNEVSSLHPSTQ 325
           MADGD ISIFGSSHIWIDHNSLSNCADGL+DAVM STAITI+NNYFTHHNE         
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNE--------- 272

Query: 326 FVFLPFFRDIASRCVFNEQVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF 385
                              VMLLGHSD+Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYF
Sbjct: 273 -------------------VMLLGHSDTYTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYF 332

Query: 386 HVVNNDYTHWEMYAIGGSANPTINSQGNRYAAPTNPFAKEVTKRVETAESQWKNWNWRSE 445
           HVVNNDYTHWEMYAIGGSA+PTINSQGNRY AP N FAKEVTKR    + QW++WNWRSE
Sbjct: 333 HVVNNDYTHWEMYAIGGSASPTINSQGNRYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSE 392

Query: 446 GDLLLNGAFFTPSGAGASASYARASSLGAKSSSMVASITSSAGALSCRRGHPC 499
           GDL LNGAFFT SG+G  ASYARASSL AKSSS+V  IT +AGAL+CR G  C
Sbjct: 393 GDLFLNGAFFTRSGSGLGASYARASSLAAKSSSLVGVITYNAGALNCRGGRRC 417

The following BLAST results are available for this feature:

BLAST of Spg031439 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022924851.1	7.3e-243	86.00	probable pectate lyase 8 [Cucurbita moschata] >KAG6582448.1 putative pectate lya...	[more]
XP_023527818.1	1.0e-241	85.20	probable pectate lyase 8 [Cucurbita pepo subsp. pepo]	[more]
XP_022974867.1	1.8e-241	84.94	probable pectate lyase 8 [Cucurbita maxima]	[more]
KAG6597311.1	5.2e-241	84.94	putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038895013.1	6.8e-241	84.94	probable pectate lyase 8 [Benincasa hispida]	[more]

BLAST of Spg031439 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q944R1	2.0e-195	75.74	Probable pectate lyase 15 OS=Arabidopsis thaliana OX=3702 GN=At4g13710 PE=2 SV=1	[more]
Q940Q1	1.3e-194	77.31	Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2	[more]
Q9M8Z8	7.2e-193	78.21	Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1	[more]
Q93WF1	1.1e-188	76.27	Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1	[more]
Q9LJ42	5.3e-188	73.78	Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2	[more]

BLAST of Spg031439 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EAL4	3.5e-243	86.00	Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111432214 PE=3 SV=1	[more]
A0A6J1IBF4	8.7e-242	84.94	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111473637 PE=3 SV=1	[more]
A0A6J1CZC3	4.3e-241	85.54	Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111016150 PE=3 SV=1	[more]
A0A6J1IVV8	9.6e-241	85.34	Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111479711 PE=3 SV=1	[more]
A0A5D3D285	3.1e-239	84.54	Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002440...	[more]

BLAST of Spg031439 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13710.1	1.4e-196	75.74	Pectin lyase-like superfamily protein	[more]
AT4G13710.2	1.4e-196	75.74	Pectin lyase-like superfamily protein	[more]
AT1G04680.1	9.3e-196	77.31	Pectin lyase-like superfamily protein	[more]
AT3G07010.1	5.1e-194	78.21	Pectin lyase-like superfamily protein	[more]
AT5G48900.1	7.6e-190	76.27	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018082	AmbAllergen	PRINTS	PR00807	AMBALLERGEN	coord: 144..161 score: 54.76 coord: 204..220 score: 63.87 coord: 168..193 score: 68.68 coord: 268..289 score: 69.48
IPR002022	Pectate lyase	SMART	SM00656	amb_all	coord: 196..421 e-value: 1.2E-91 score: 320.4
IPR002022	Pectate lyase	PFAM	PF00544	Pectate_lyase_4	coord: 341..413 e-value: 1.0E-7 score: 31.8 coord: 207..317 e-value: 6.5E-6 score: 25.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 121..491 e-value: 4.0E-111 score: 373.3
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 77..323
IPR045032	Pectin lyase family	PANTHER	PTHR31683	PECTATE LYASE 18-RELATED	coord: 342..495
None	No IPR available	PANTHER	PTHR31683:SF81	PECTATE LYASE	coord: 77..323 coord: 342..495
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 98..493

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spg031439.1	Spg031439.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030570	pectate lyase activity