Spg031391 (gene) Sponge gourd (cylindrica) v1

Overview
NameSpg031391
Typegene
OrganismLuffa cylindrica (Sponge gourd (cylindrica) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationscaffold11: 42897268 .. 42898365 (+)
RNA-Seq ExpressionSpg031391
SyntenySpg031391
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACATGGCACTCACTTACTCCACCGCCATCTTCTTCAAAACCAACCACCGAATTCTCAGCCCACCACCAACAATCATCTTCTTCTTCTTCTCCAATGGCGGAATCCCTCTCTGCAACTGCCACAACCTCCATTATCGATCCCTTCGAGCATCTCAACATCAAGCTCAACCCAGACGGCTCAATCACCAGACCCAACCAATTCCCCACACTACCCCCAACACAAACTCCTATTCCCAATATTGACCCTGCAATTTCCAAAGACCTTCCCTTAAAACCTTCCAACAATACCTTCATTCGCCTCTACAAGCCCCACAATATTCCTCAAAACGCGATCAATAAACTTCCCATTGTGGTCTACGGCCATGGCGGCGGCTTCCTTTTCTTCAGCGTCGCATCAGAGCCATTTCACCACTGGTGTTCTCGCATGTCGGCTCATCTTCCAGCCCTCATCGTGTCGTTCGAGTACCGCCTAGCCCCCGAGCACCGTCTCCCGGCGGCGTACGACGACGCCGTCGAGGCCATCGGGTGGCTCCGTCGCCAGGCCGATGGAATCGACAGCGGCGATTGCGAGCCGTGGTTGAAAGAGTGTGCTGACTTTTCGCGGTGTTTCTTGTTCGGCAGTAGCTCCGGCGGGAACATCACGTTCAATGCTGCTGTACGAGCACTGGACTGCGATCTCTCGCCGGTTAAAATCGTGGGGCATGTTTTTAACCAGCCGTTTTTCGGTGGGATTGAACGGACGGAGTCGGAGCTCCGGTCGGCGAACGACCGGATCTTGCCGTTGCCGGTGAATGATTTAATGTGGGGTTTGGCGCTGCCGGAGGATTGTGATCGGAGTCACTGGTTTTGTGATCCGACGGTGGAGGGGCCGCACCAAGAGAAGATCGGACGGCTGCGGAGGTGCTTGGTGAGAGGACATGGTGGTGATCCGTTGATTGACAGGCAGAAGGAGTTTGTGAAGCTGCTGGAGGCACGTGGGGTGCACGTGGAGTCCAGATTTCACGAGGGAGGGTTTCATGGCGTGGAGCTATTTGATCCGTCCAAACTTCAAACGTTAGTGGATGATGTGAAAGATTTCATTGATGCTTCTAAGTAG

mRNA sequence

ATGACATGGCACTCACTTACTCCACCGCCATCTTCTTCAAAACCAACCACCGAATTCTCAGCCCACCACCAACAATCATCTTCTTCTTCTTCTCCAATGGCGGAATCCCTCTCTGCAACTGCCACAACCTCCATTATCGATCCCTTCGAGCATCTCAACATCAAGCTCAACCCAGACGGCTCAATCACCAGACCCAACCAATTCCCCACACTACCCCCAACACAAACTCCTATTCCCAATATTGACCCTGCAATTTCCAAAGACCTTCCCTTAAAACCTTCCAACAATACCTTCATTCGCCTCTACAAGCCCCACAATATTCCTCAAAACGCGATCAATAAACTTCCCATTGTGGTCTACGGCCATGGCGGCGGCTTCCTTTTCTTCAGCGTCGCATCAGAGCCATTTCACCACTGGTGTTCTCGCATGTCGGCTCATCTTCCAGCCCTCATCGTGTCGTTCGAGTACCGCCTAGCCCCCGAGCACCGTCTCCCGGCGGCGTACGACGACGCCGTCGAGGCCATCGGGTGGCTCCGTCGCCAGGCCGATGGAATCGACAGCGGCGATTGCGAGCCGTGGTTGAAAGAGTGTGCTGACTTTTCGCGGTGTTTCTTGTTCGGCAGTAGCTCCGGCGGGAACATCACGTTCAATGCTGCTGTACGAGCACTGGACTGCGATCTCTCGCCGGTTAAAATCGTGGGGCATGTTTTTAACCAGCCGTTTTTCGGTGGGATTGAACGGACGGAGTCGGAGCTCCGGTCGGCGAACGACCGGATCTTGCCGTTGCCGGTGAATGATTTAATGTGGGGTTTGGCGCTGCCGGAGGATTGTGATCGGAGTCACTGGTTTTGTGATCCGACGGTGGAGGGGCCGCACCAAGAGAAGATCGGACGGCTGCGGAGGTGCTTGGTGAGAGGACATGGTGGTGATCCGTTGATTGACAGGCAGAAGGAGTTTGTGAAGCTGCTGGAGGCACGTGGGGTGCACGTGGAGTCCAGATTTCACGAGGGAGGGTTTCATGGCGTGGAGCTATTTGATCCGTCCAAACTTCAAACGTTAGTGGATGATGTGAAAGATTTCATTGATGCTTCTAAGTAG

Coding sequence (CDS)

ATGACATGGCACTCACTTACTCCACCGCCATCTTCTTCAAAACCAACCACCGAATTCTCAGCCCACCACCAACAATCATCTTCTTCTTCTTCTCCAATGGCGGAATCCCTCTCTGCAACTGCCACAACCTCCATTATCGATCCCTTCGAGCATCTCAACATCAAGCTCAACCCAGACGGCTCAATCACCAGACCCAACCAATTCCCCACACTACCCCCAACACAAACTCCTATTCCCAATATTGACCCTGCAATTTCCAAAGACCTTCCCTTAAAACCTTCCAACAATACCTTCATTCGCCTCTACAAGCCCCACAATATTCCTCAAAACGCGATCAATAAACTTCCCATTGTGGTCTACGGCCATGGCGGCGGCTTCCTTTTCTTCAGCGTCGCATCAGAGCCATTTCACCACTGGTGTTCTCGCATGTCGGCTCATCTTCCAGCCCTCATCGTGTCGTTCGAGTACCGCCTAGCCCCCGAGCACCGTCTCCCGGCGGCGTACGACGACGCCGTCGAGGCCATCGGGTGGCTCCGTCGCCAGGCCGATGGAATCGACAGCGGCGATTGCGAGCCGTGGTTGAAAGAGTGTGCTGACTTTTCGCGGTGTTTCTTGTTCGGCAGTAGCTCCGGCGGGAACATCACGTTCAATGCTGCTGTACGAGCACTGGACTGCGATCTCTCGCCGGTTAAAATCGTGGGGCATGTTTTTAACCAGCCGTTTTTCGGTGGGATTGAACGGACGGAGTCGGAGCTCCGGTCGGCGAACGACCGGATCTTGCCGTTGCCGGTGAATGATTTAATGTGGGGTTTGGCGCTGCCGGAGGATTGTGATCGGAGTCACTGGTTTTGTGATCCGACGGTGGAGGGGCCGCACCAAGAGAAGATCGGACGGCTGCGGAGGTGCTTGGTGAGAGGACATGGTGGTGATCCGTTGATTGACAGGCAGAAGGAGTTTGTGAAGCTGCTGGAGGCACGTGGGGTGCACGTGGAGTCCAGATTTCACGAGGGAGGGTTTCATGGCGTGGAGCTATTTGATCCGTCCAAACTTCAAACGTTAGTGGATGATGTGAAAGATTTCATTGATGCTTCTAAGTAG

Protein sequence

MTWHSLTPPPSSSKPTTEFSAHHQQSSSSSSPMAESLSATATTSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYRLAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGGNITFNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPEDCDRSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFHEGGFHGVELFDPSKLQTLVDDVKDFIDASK
Homology
BLAST of Spg031391 vs. NCBI nr
Match: XP_023526606.1 (probable carboxylesterase 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 570.1 bits (1468), Expect = 1.4e-158
Identity = 268/335 (80.00%), Postives = 295/335 (88.06%), Query Frame = 0

Query: 33  MAESLSATATTSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIP---NIDPAISKDL 92
           MAESLSATATTSIIDPFEHLNIKLNPDGSITR NQFPT+PPT+TP P   N+    SKD+
Sbjct: 1   MAESLSATATTSIIDPFEHLNIKLNPDGSITRHNQFPTVPPTETPTPNTNNLSTCCSKDI 60

Query: 93  PLKPSNNTFIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPA 152
           PL PSNNTFIRL+KPHNIPQN  NKLPI++  HGGGF+ FS A++ FH  C R+SAHLPA
Sbjct: 61  PLNPSNNTFIRLFKPHNIPQNPTNKLPILIDAHGGGFILFSAATQLFHDTCCRLSAHLPA 120

Query: 153 LIVSFEYRLAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSS 212
           LIVSFEYRLAPEHRLPAAYDDA+EA+ WL RQA+ I  GDC+PWLK+CADFSRCFL G+S
Sbjct: 121 LIVSFEYRLAPEHRLPAAYDDALEALRWLCRQANQIHGGDCDPWLKDCADFSRCFLLGTS 180

Query: 213 SGGNITFNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMW 272
           +GGNITF AA+RALD DLSPVKIVGH++NQPFFGGI+RTESELR  NDRILPL VNDLMW
Sbjct: 181 AGGNITFYAALRALDSDLSPVKIVGHIYNQPFFGGIKRTESELRCVNDRILPLSVNDLMW 240

Query: 273 GLALPEDCDRSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVH 332
            LALPE CDR HWFCDPTVEGPHQEKI RL+RCLVRGH GDPLIDRQKEFVKLLEARGV 
Sbjct: 241 SLALPEGCDRDHWFCDPTVEGPHQEKIRRLQRCLVRGHVGDPLIDRQKEFVKLLEARGVK 300

Query: 333 VESRFHEGGFHGVELFDPSKLQTLVDDVKDFIDAS 365
           V++RF EGG+HGV+LFDPSKLQ L DDVKDFIDAS
Sbjct: 301 VDARFREGGYHGVDLFDPSKLQILEDDVKDFIDAS 335

BLAST of Spg031391 vs. NCBI nr
Match: XP_022924288.1 (probable carboxylesterase 8 [Cucurbita moschata])

HSP 1 Score: 566.2 bits (1458), Expect = 2.0e-157
Identity = 266/335 (79.40%), Postives = 293/335 (87.46%), Query Frame = 0

Query: 33  MAESLSATATTSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIP---NIDPAISKDL 92
           MAESLSATAT SIIDPFEHLNIKLNPDGSITR NQ PT+PPT+TP P   N+    SKD+
Sbjct: 1   MAESLSATATASIIDPFEHLNIKLNPDGSITRHNQLPTVPPTETPTPNTNNLSTCCSKDI 60

Query: 93  PLKPSNNTFIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPA 152
           PL PSNNTFIRL+KPHNIPQN  NKLPI++  HGGGF+ FS A++ FH  C R+SAHLPA
Sbjct: 61  PLNPSNNTFIRLFKPHNIPQNPTNKLPILIDAHGGGFILFSAATQLFHETCCRLSAHLPA 120

Query: 153 LIVSFEYRLAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSS 212
           LIVSFEYRLAPEHRLPAAYDDA+EA+ WL RQA+ I  GDC+PWLK+CADFSRCFL G+S
Sbjct: 121 LIVSFEYRLAPEHRLPAAYDDALEALRWLCRQANQIHGGDCDPWLKDCADFSRCFLLGTS 180

Query: 213 SGGNITFNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMW 272
           +GGNITF AA+RALD DLSPVKIVGH++NQPFFGGI+RTESELR  NDRILPL VNDLMW
Sbjct: 181 AGGNITFYAALRALDSDLSPVKIVGHIYNQPFFGGIKRTESELRCVNDRILPLSVNDLMW 240

Query: 273 GLALPEDCDRSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVH 332
            LALPE CDR HWFCDPTVEGPHQEKI RL+RCLVRGH GDPLIDRQKEFVKLLEARGV 
Sbjct: 241 SLALPEGCDRDHWFCDPTVEGPHQEKIRRLQRCLVRGHVGDPLIDRQKEFVKLLEARGVK 300

Query: 333 VESRFHEGGFHGVELFDPSKLQTLVDDVKDFIDAS 365
           V++RF EGG+HGV+LFDPSKLQ L DDVKDFIDAS
Sbjct: 301 VDARFREGGYHGVDLFDPSKLQILEDDVKDFIDAS 335

BLAST of Spg031391 vs. NCBI nr
Match: KAG6582576.1 (putative carboxylesterase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 511.5 bits (1316), Expect = 5.9e-141
Identity = 246/332 (74.10%), Postives = 268/332 (80.72%), Query Frame = 0

Query: 33  MAESLSATATTSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLK 92
           MAESLSATATTSIIDPFEHLNIKLNPDGSITR NQFPT                      
Sbjct: 1   MAESLSATATTSIIDPFEHLNIKLNPDGSITRHNQFPT---------------------- 60

Query: 93  PSNNTFIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIV 152
                      PHNIPQN  NKLPI++  HGGGF+ FS A++ FH  C R+SAHLPALIV
Sbjct: 61  -----------PHNIPQNPTNKLPILIDAHGGGFILFSAATQLFHETCCRLSAHLPALIV 120

Query: 153 SFEYRLAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGG 212
           SFEYRLAPEHRLPAAYDDA+EA+ WL RQA+ I  GDC+PWLK+CADFSRCFL G+S+GG
Sbjct: 121 SFEYRLAPEHRLPAAYDDALEALRWLCRQANQIHGGDCDPWLKDCADFSRCFLLGTSAGG 180

Query: 213 NITFNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLA 272
           NITF AA+RALD DLSPVKIVGH++NQPFFGGI+RTESELR  NDRILPL VNDLMW LA
Sbjct: 181 NITFYAALRALDSDLSPVKIVGHIYNQPFFGGIKRTESELRCVNDRILPLSVNDLMWSLA 240

Query: 273 LPEDCDRSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVES 332
           LPE CDR HWFCDPTVEGPHQEKI RL+RCLVRGH GDPLIDRQKEFVKLLEARGV V++
Sbjct: 241 LPEGCDRDHWFCDPTVEGPHQEKIRRLQRCLVRGHVGDPLIDRQKEFVKLLEARGVKVDA 299

Query: 333 RFHEGGFHGVELFDPSKLQTLVDDVKDFIDAS 365
           RF EGG+HGV+LFDPSKLQ L DDVKDFIDAS
Sbjct: 301 RFREGGYHGVDLFDPSKLQILEDDVKDFIDAS 299

BLAST of Spg031391 vs. NCBI nr
Match: XP_030930396.1 (probable carboxylesterase 8 [Quercus lobata])

HSP 1 Score: 422.5 bits (1085), Expect = 3.6e-114
Identity = 201/326 (61.66%), Postives = 242/326 (74.23%), Query Frame = 0

Query: 43  TSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDP-----AISKDLPLKPSNNT 102
           TS IDP++ LNI  NPDGS+TR   F   P +  P P+ DP     A+SKD+PL P   T
Sbjct: 7   TSNIDPYKFLNISPNPDGSLTRLTPFS--PASTNPEPSKDPSTQLLALSKDIPLNPKTKT 66

Query: 103 FIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYR 162
           F+RL+ PH +PQN   KLP+++Y HGGGF+ FS AS+PFH  CS M+A  PALIVS EYR
Sbjct: 67  FLRLFLPHPVPQNQNQKLPVIIYFHGGGFVLFSAASQPFHDSCSNMAAQFPALIVSVEYR 126

Query: 163 LAPEHRLPAAYDDAVEAIGWLRRQA--DGIDSGDCEPWLKECADFSRCFLFGSSSGGNIT 222
           LAPEHRLPAAYDDA++AI W+R QA  D  D   C+ WL+  ADFSRCFL GSS+GGNI 
Sbjct: 127 LAPEHRLPAAYDDAMDAIMWIRNQAKKDEDDINGCDAWLRRYADFSRCFLMGSSAGGNIV 186

Query: 223 FNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPE 282
           ++A +RALD D+ PV+I G + NQP+FGG++RT SE+R  NDRILPL  NDLMW LALP+
Sbjct: 187 YHAGLRALDVDIKPVEIKGLILNQPYFGGVQRTGSEIRLINDRILPLTANDLMWALALPK 246

Query: 283 DCDRSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFH 342
           D DR H +C+PTV G H  KIGRL RCLVRG+GGDPL DRQKE VK+LEARGVHV + F 
Sbjct: 247 DADRDHEYCNPTVGGSHDAKIGRLPRCLVRGYGGDPLQDRQKELVKILEARGVHVVAEFD 306

Query: 343 EGGFHGVELFDPSKLQTLVDDVKDFI 362
           E GFH VELFDP+K Q L   ++DFI
Sbjct: 307 EEGFHAVELFDPTKAQALFKGIRDFI 330

BLAST of Spg031391 vs. NCBI nr
Match: XP_023928443.1 (probable carboxylesterase 8 [Quercus suber])

HSP 1 Score: 421.4 bits (1082), Expect = 8.0e-114
Identity = 203/328 (61.89%), Postives = 246/328 (75.00%), Query Frame = 0

Query: 43  TSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDP-----AISKDLPLKPSNNT 102
           TS IDP++ LNI  NPDGS+TR   FP  P +  P P+ DP     A+SKD+PL P   T
Sbjct: 7   TSNIDPYKFLNISPNPDGSLTRLTPFP--PASTNPEPSKDPSTQLLALSKDIPLNPKTKT 66

Query: 103 FIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYR 162
           F+RL+ PH +PQN   KLP+++Y HGGGF+FFS AS+PFH  CS M+A  PALIVS EYR
Sbjct: 67  FLRLFLPHPVPQN--QKLPVIIYFHGGGFVFFSAASQPFHDSCSNMAAQFPALIVSVEYR 126

Query: 163 LAPEHRLPAAYDDAVEAIGWLRRQA----DGIDSGDCEPWLKECADFSRCFLFGSSSGGN 222
           LAPEHRLPAAYDDA++AI WLR QA    DGI+   C+ WL++  DFSRCFL GSS+GGN
Sbjct: 127 LAPEHRLPAAYDDAMDAIMWLRNQARKDEDGING--CDAWLRQYVDFSRCFLMGSSAGGN 186

Query: 223 ITFNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLAL 282
           I ++A +RALD D+ PV+I G + NQP+FGG++RT SE+R  +DRILPL  NDLMW LAL
Sbjct: 187 IVYHAGLRALDVDIKPVEIKGLILNQPYFGGVQRTGSEIRLIDDRILPLTANDLMWALAL 246

Query: 283 PEDCDRSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESR 342
           P+D DR H +C+PTV G H  KIGRL RCLVRG+GGDPL DRQKE VK+LEARGVHV + 
Sbjct: 247 PKDADRDHEYCNPTVGGSHDAKIGRLPRCLVRGYGGDPLQDRQKELVKILEARGVHVVAE 306

Query: 343 FHEGGFHGVELFDPSKLQTLVDDVKDFI 362
           F E GFH VELFDP+K Q L   ++DFI
Sbjct: 307 FDEEGFHAVELFDPTKAQALFKGIRDFI 328

BLAST of Spg031391 vs. ExPASy Swiss-Prot
Match: O64640 (Probable carboxylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=CXE8 PE=2 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.7e-98
Identity = 172/318 (54.09%), Postives = 226/318 (71.07%), Query Frame = 0

Query: 47  DPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYKPHN 106
           DP++ LNI LN DGS+TR   FP LPPT+          SKD+PL  +NNTFIR++KP N
Sbjct: 9   DPYKFLNITLNSDGSLTRHRDFPKLPPTEQ---------SKDIPLNQTNNTFIRIFKPRN 68

Query: 107 IPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYRLAPEHRLPA 166
           IP    +KLPI+VY HGGGF+ +S AS PFH  C++M+  L  +I+S EYRLAPEHRLPA
Sbjct: 69  IPPE--SKLPILVYFHGGGFILYSAASAPFHESCTKMADRLQTIILSVEYRLAPEHRLPA 128

Query: 167 AYDDAVEAIGWLRRQADG-IDSGDCEPWLKECADFSRCFLFGSSSGGNITFNAAVRALDC 226
           AY+DAVEAI WLR QA G I+ GDC+ WLK+  DFS+C++ GSSSGGNI +N A+R +D 
Sbjct: 129 AYEDAVEAILWLRDQARGPINGGDCDTWLKDGVDFSKCYVMGSSSGGNIVYNVALRVVDT 188

Query: 227 DLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPEDCDRSHWFCD 286
           DLSPVKI G + NQ FFGG+E ++SE R  +D+I PLP   L+W L LP+  DR H + +
Sbjct: 189 DLSPVKIQGLIMNQAFFGGVEPSDSESRLKDDKICPLPATHLLWSLCLPDGVDRDHVYSN 248

Query: 287 P-TVEGPHQ-EKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFHEGGFHGVE 346
           P    GP + +K+GR    L+ G+GGDPL+DRQ+   ++L+ RGVHVE+RF + GFH  E
Sbjct: 249 PIKSSGPQEKDKMGRFPSTLINGYGGDPLVDRQRHVAEMLKGRGVHVETRFDKDGFHACE 308

Query: 347 LFDPSKLQTLVDDVKDFI 362
           LFD +K + L + V+ F+
Sbjct: 309 LFDGNKAKALYETVEAFM 315

BLAST of Spg031391 vs. ExPASy Swiss-Prot
Match: O64641 (Probable carboxylesterase 9 OS=Arabidopsis thaliana OX=3702 GN=CXE9 PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 7.3e-86
Identity = 150/317 (47.32%), Postives = 216/317 (68.14%), Query Frame = 0

Query: 47  DPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYKPHN 106
           DP++HLNI +NP+GS TR   +P + P   P P    A SKD+ +       +R+++P N
Sbjct: 10  DPYKHLNITINPNGSCTRHFVWPRVEPDPDPCPG-KLAASKDVTINHETGVSVRIFRPTN 69

Query: 107 IP--QNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYRLAPEHRL 166
           +P   NA+ +LPI+++ HG G++ +   S      CS+M++ L  ++VS  YRL PEHRL
Sbjct: 70  LPSNDNAVARLPIIIHLHGSGWILYPANSAANDRCCSQMASELTVIVVSVHYRLPPEHRL 129

Query: 167 PAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGGNITFNAAVRALD 226
           PA YDDA++A+ W+++Q   +DS + EPWLK+ ADFSRC++ GSS+G NI F  A+R+LD
Sbjct: 130 PAQYDDALDALLWVKQQV--VDSTNGEPWLKDYADFSRCYICGSSNGANIAFQLALRSLD 189

Query: 227 CDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPEDCDRSHWFC 286
            DL+P++I G VF QP FGG  RT+SEL++  D ++P+P  D MW L+LP   DR H +C
Sbjct: 190 HDLTPLQIDGCVFYQPLFGGKTRTKSELKNFADPVMPVPAVDAMWELSLPVGVDRDHRYC 249

Query: 287 DPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFHEGGFHGVEL 346
           +P    P +EK+GRL RCLV G+GGD  +DRQ++FV LL A GV VE+RF + GFH +EL
Sbjct: 250 NPLGYLPQKEKVGRLGRCLVIGYGGDTSLDRQQDFVNLLVAAGVRVEARFDDAGFHSIEL 309

Query: 347 FDPSKLQTLVDDVKDFI 362
            DP +   L++ ++DFI
Sbjct: 310 VDPRRAVALLNMIRDFI 323

BLAST of Spg031391 vs. ExPASy Swiss-Prot
Match: Q9LVB8 (Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.3e-78
Identity = 157/330 (47.58%), Postives = 215/330 (65.15%), Query Frame = 0

Query: 45  IIDPFEHLNIKLNPDGSITRP-NQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYK 104
           I DP+ +LNI  NPDGSITR  + FP    T  P P ++PA+SKDLP+    +T++RLY 
Sbjct: 7   IADPYAYLNIVNNPDGSITRDLSNFPCTAATPDPSP-LNPAVSKDLPVNQLKSTWLRLY- 66

Query: 105 PHNIPQNAIN-------KLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEY 164
              +P +A+N       KLPIVVY HGGGF+  SV  + FH +CS ++  L A++VS  Y
Sbjct: 67  ---LPSSAVNEGNVSSQKLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSY 126

Query: 165 RLAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGGNITF 224
           RLAPEHRLPAAYDD VEA+ W++   D         W+K  ADFS  FL G+S+GGN+ +
Sbjct: 127 RLAPEHRLPAAYDDGVEALDWIKTSDD--------EWIKSHADFSNVFLMGTSAGGNLAY 186

Query: 225 NAAVRALD--CDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALP 284
           N  +R++D   DLSP++I G + + PFFGG ER+ESE+R  ND++ P  V D+MW L+LP
Sbjct: 187 NVGLRSVDSVSDLSPLQIRGLILHHPFFGGEERSESEIRLMNDQVCPPIVTDVMWDLSLP 246

Query: 285 EDCDRSHWFCDPTV-EGPHQ-EKIGRLR-RCLVRGHGGDPLIDRQKEFVKLLEARGVHVE 344
              DR H + +PTV +G  + EKIGRLR + ++ G   DP+ID QK+  KL++ +GV V 
Sbjct: 247 VGVDRDHEYSNPTVGDGSEKLEKIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVEVV 306

Query: 345 SRFHEGGFHGVELFDPSKLQTLVDDVKDFI 362
             +  G  HG E+ DPSK +TL   +K+FI
Sbjct: 307 EHYTGGHVHGAEIRDPSKRKTLFLSIKNFI 323

BLAST of Spg031391 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.4e-75
Identity = 154/330 (46.67%), Postives = 205/330 (62.12%), Query Frame = 0

Query: 42  TTSIIDP----FEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNT 101
           TT   DP     ++L I LNPD +ITRP Q P+   +  P  +  P ++KDL L P +NT
Sbjct: 8   TTGSSDPNTNLLKYLPIVLNPDRTITRPIQIPSTAASPDPTSS-SPVLTKDLALNPLHNT 67

Query: 102 FIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYR 161
           F+RL+ P +   N+  KLP+VVY HGGGF+ FS AS  FH +C  M+ H   +I S +YR
Sbjct: 68  FVRLFLPRHALYNSA-KLPLVVYFHGGGFILFSAASTIFHDFCCEMAVHAGVVIASVDYR 127

Query: 162 LAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGGNITFN 221
           LAPEHRLPAAYDDA+EA+ W++      DS D   WL   ADFS CF+ G S+GGNI ++
Sbjct: 128 LAPEHRLPAAYDDAMEALQWIK------DSRD--EWLTNFADFSNCFIMGESAGGNIAYH 187

Query: 222 AAVR--ALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPE 281
           A +R  A+  +L P+KI G V ++P FGG +RT SELR AND  LP  V DL+W L+LP 
Sbjct: 188 AGLRAAAVADELLPLKIKGLVLDEPGFGGSKRTGSELRLANDSRLPTFVLDLIWELSLPM 247

Query: 282 DCDRSHWFCDPTVEGPHQEKIGRLR----RCLVRGHGGDPLIDRQKEFVKLLEARGVHVE 341
             DR H +C+PT E        ++R    R +V G  GDP+IDRQ E  + LE +GV V 
Sbjct: 248 GADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGDPMIDRQMELAERLEKKGVDVV 307

Query: 342 SRFHEGGFHGVELFDPSKLQTLVDDVKDFI 362
           ++F  GG+H V+L DP K +     +K F+
Sbjct: 308 AQFDVGGYHAVKLEDPEKAKQFFVILKKFV 327

BLAST of Spg031391 vs. ExPASy Swiss-Prot
Match: I3PLR2 (3-O-acetylpapaveroxine carboxylesterase CXE1 OS=Papaver somniferum OX=3469 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 9.9e-75
Identity = 140/321 (43.61%), Postives = 199/321 (61.99%), Query Frame = 0

Query: 47  DPFEHLNIKLNP-DGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYKP- 106
           DP+E L    NP + ++TR    P  P  Q          +KD+ L P   T +R+++P 
Sbjct: 3   DPYEFLMCIHNPEEDTLTRNFPIPATPLDQN---------TKDISLNPDRKTSLRIFRPP 62

Query: 107 -HNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYRLAPEHR 166
               P      LPI++Y HGGGF+ F+  S   H +C  ++ H+PAL+VS +YRLAPE+R
Sbjct: 63  TKEPPVTKNKLLPIIIYFHGGGFILFNADSTMNHDFCQSIATHIPALVVSVDYRLAPENR 122

Query: 167 LPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGGNITFNAAVRAL 226
           LPAAYDDAV+A+ W++ Q  G    + E WLKE  DFS+CF+ G SSG N+ ++A++RA+
Sbjct: 123 LPAAYDDAVDALNWVKDQGLG-KLNNSEVWLKEYGDFSKCFIMGCSSGANVAYHASLRAI 182

Query: 227 DCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPEDCDRSHWF 286
           + DL P KI G + + PFFG +ERTES+ +  N++ LPL V D+MW LALP    R H +
Sbjct: 183 EMDLEPAKINGLILHCPFFGSLERTESDSKVINNQDLPLAVRDVMWELALPLGSTRDHVY 242

Query: 287 CDPTVE---GPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFHEGGFH 346
           C+P ++         +G + RC V G  GDPLIDRQ + VK+LE +GV VE+   +GG+H
Sbjct: 243 CNPNIDHDGSSSGNMVGLIERCFVVGFYGDPLIDRQIQLVKMLEEKGVKVETWIEQGGYH 302

Query: 347 GVELFDPSKLQTLVDDVKDFI 362
           GV  FDP   +T ++ +K FI
Sbjct: 303 GVLCFDPMIRETFLEKLKHFI 313

BLAST of Spg031391 vs. ExPASy TrEMBL
Match: A0A6J1EC01 (probable carboxylesterase 8 OS=Cucurbita moschata OX=3662 GN=LOC111431817 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 9.7e-158
Identity = 266/335 (79.40%), Postives = 293/335 (87.46%), Query Frame = 0

Query: 33  MAESLSATATTSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIP---NIDPAISKDL 92
           MAESLSATAT SIIDPFEHLNIKLNPDGSITR NQ PT+PPT+TP P   N+    SKD+
Sbjct: 1   MAESLSATATASIIDPFEHLNIKLNPDGSITRHNQLPTVPPTETPTPNTNNLSTCCSKDI 60

Query: 93  PLKPSNNTFIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPA 152
           PL PSNNTFIRL+KPHNIPQN  NKLPI++  HGGGF+ FS A++ FH  C R+SAHLPA
Sbjct: 61  PLNPSNNTFIRLFKPHNIPQNPTNKLPILIDAHGGGFILFSAATQLFHETCCRLSAHLPA 120

Query: 153 LIVSFEYRLAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSS 212
           LIVSFEYRLAPEHRLPAAYDDA+EA+ WL RQA+ I  GDC+PWLK+CADFSRCFL G+S
Sbjct: 121 LIVSFEYRLAPEHRLPAAYDDALEALRWLCRQANQIHGGDCDPWLKDCADFSRCFLLGTS 180

Query: 213 SGGNITFNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMW 272
           +GGNITF AA+RALD DLSPVKIVGH++NQPFFGGI+RTESELR  NDRILPL VNDLMW
Sbjct: 181 AGGNITFYAALRALDSDLSPVKIVGHIYNQPFFGGIKRTESELRCVNDRILPLSVNDLMW 240

Query: 273 GLALPEDCDRSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVH 332
            LALPE CDR HWFCDPTVEGPHQEKI RL+RCLVRGH GDPLIDRQKEFVKLLEARGV 
Sbjct: 241 SLALPEGCDRDHWFCDPTVEGPHQEKIRRLQRCLVRGHVGDPLIDRQKEFVKLLEARGVK 300

Query: 333 VESRFHEGGFHGVELFDPSKLQTLVDDVKDFIDAS 365
           V++RF EGG+HGV+LFDPSKLQ L DDVKDFIDAS
Sbjct: 301 VDARFREGGYHGVDLFDPSKLQILEDDVKDFIDAS 335

BLAST of Spg031391 vs. ExPASy TrEMBL
Match: A0A7N2MDV0 (Abhydrolase_3 domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 1.7e-114
Identity = 201/326 (61.66%), Postives = 242/326 (74.23%), Query Frame = 0

Query: 43  TSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDP-----AISKDLPLKPSNNT 102
           TS IDP++ LNI  NPDGS+TR   F   P +  P P+ DP     A+SKD+PL P   T
Sbjct: 7   TSNIDPYKFLNISPNPDGSLTRLTPFS--PASTNPEPSKDPSTQLLALSKDIPLNPKTKT 66

Query: 103 FIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYR 162
           F+RL+ PH +PQN   KLP+++Y HGGGF+ FS AS+PFH  CS M+A  PALIVS EYR
Sbjct: 67  FLRLFLPHPVPQNQNQKLPVIIYFHGGGFVLFSAASQPFHDSCSNMAAQFPALIVSVEYR 126

Query: 163 LAPEHRLPAAYDDAVEAIGWLRRQA--DGIDSGDCEPWLKECADFSRCFLFGSSSGGNIT 222
           LAPEHRLPAAYDDA++AI W+R QA  D  D   C+ WL+  ADFSRCFL GSS+GGNI 
Sbjct: 127 LAPEHRLPAAYDDAMDAIMWIRNQAKKDEDDINGCDAWLRRYADFSRCFLMGSSAGGNIV 186

Query: 223 FNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPE 282
           ++A +RALD D+ PV+I G + NQP+FGG++RT SE+R  NDRILPL  NDLMW LALP+
Sbjct: 187 YHAGLRALDVDIKPVEIKGLILNQPYFGGVQRTGSEIRLINDRILPLTANDLMWALALPK 246

Query: 283 DCDRSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFH 342
           D DR H +C+PTV G H  KIGRL RCLVRG+GGDPL DRQKE VK+LEARGVHV + F 
Sbjct: 247 DADRDHEYCNPTVGGSHDAKIGRLPRCLVRGYGGDPLQDRQKELVKILEARGVHVVAEFD 306

Query: 343 EGGFHGVELFDPSKLQTLVDDVKDFI 362
           E GFH VELFDP+K Q L   ++DFI
Sbjct: 307 EEGFHAVELFDPTKAQALFKGIRDFI 330

BLAST of Spg031391 vs. ExPASy TrEMBL
Match: A0A6P4AD25 (probable carboxylesterase 8 OS=Ziziphus jujuba OX=326968 GN=LOC107427168 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.5e-110
Identity = 201/339 (59.29%), Postives = 258/339 (76.11%), Query Frame = 0

Query: 33  MAESLSATATTSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDPA---ISKDL 92
           MAE  S+++++S ID ++ L+I  NPDGS+TR   FP +PP+ + I N +P+   ISKDL
Sbjct: 1   MAEPPSSSSSSSSIDAYKLLHIVPNPDGSLTRLTPFPVVPPSDS-ITNTNPSPFTISKDL 60

Query: 93  PLKPSNNTFIRLYKPHNIPQNAINK--LPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHL 152
           PL  +N TF+RL+KP N+P N  N   LP+++Y HGGGF+ FS AS PFH  CSRM+ HL
Sbjct: 61  PLNSTNKTFLRLFKPLNLPTNPNNNNLLPLIIYFHGGGFVLFSAASLPFHESCSRMALHL 120

Query: 153 PALIVSFEYRLAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFG 212
           PALI+S EYRLAPEHRLPAAYDDAVEAI W+R+QA  I+   C+PWL++CADFSRCFL G
Sbjct: 121 PALILSLEYRLAPEHRLPAAYDDAVEAILWVRQQAKDING--CDPWLRDCADFSRCFLMG 180

Query: 213 SSSGGNITFNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDL 272
           SS+GGNI ++AA+RALD DLSP+ I G + NQP+FGG++RTESE+R  +DRILPLP NDL
Sbjct: 181 SSAGGNIAYHAALRALDLDLSPLDIRGMILNQPYFGGVQRTESEIRLFDDRILPLPANDL 240

Query: 273 MWGLALPEDCDRSHWFCDP--TVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEA 332
           +W LALPE CDR H + +P  T  G    KIGRL+RCLVRG+GGDPL+DRQ+EFV++L+ 
Sbjct: 241 LWELALPEGCDRDHEYSNPMATNGGSSGGKIGRLQRCLVRGYGGDPLVDRQREFVEMLKG 300

Query: 333 RGVHVESRFHEGGFHGVELFDPSKLQTLVDDVKDFIDAS 365
           RGV V + F EGG HG ELFD +K +      ++FI+ S
Sbjct: 301 RGVEVVAEFDEGGSHGAELFDVAKAKAFYIISQNFINNS 336

BLAST of Spg031391 vs. ExPASy TrEMBL
Match: A0A1Q3C963 (Abhydrolase_3 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_20135 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 3.4e-110
Identity = 195/329 (59.27%), Postives = 245/329 (74.47%), Query Frame = 0

Query: 38  SATATTSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDP--AISKDLPLKPSN 97
           S  A TS IDP+  L I  NPDGS+TR    PT+PPT     +  P  A+SKD+PL P N
Sbjct: 5   SPPADTS-IDPYASLKISPNPDGSVTRYTPIPTVPPTPQLSDSNSPQLALSKDIPLNPKN 64

Query: 98  NTFIRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFE 157
           NTF+RLYKP      + NKLP+V+Y HGGGF+ FS AS  FH  CSRM++  PA I+S +
Sbjct: 65  NTFLRLYKPLCNNPPSTNKLPLVIYFHGGGFVVFSAASMIFHESCSRMASQFPAFILSVD 124

Query: 158 YRLAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGGNIT 217
           YRLAPEHRLPAAYDDA++AI W+  QA  ID   C+PWL++C DFS+CFL GSSSGGNI 
Sbjct: 125 YRLAPEHRLPAAYDDALDAIMWVHNQAKDIDG--CDPWLRDCVDFSKCFLMGSSSGGNIV 184

Query: 218 FNAAVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPE 277
           ++A +R+LD +LSPVKIVG + NQPFFGG+ RTESE+R  ND+I+PL V+DL+W L+LP+
Sbjct: 185 YHAGLRSLDVNLSPVKIVGLIMNQPFFGGVIRTESEMRLINDKIVPLCVSDLLWALSLPK 244

Query: 278 DCDRSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFH 337
           D +R H FCDPTV G H EKIGRL  CLV G+GGDPL+DRQKEFVK+L++R VHVE +F 
Sbjct: 245 DANRDHEFCDPTVHGSHHEKIGRLPSCLVIGYGGDPLVDRQKEFVKMLKSREVHVEEQFD 304

Query: 338 EGGFHGVELFDPSKLQTLVDDVKDFIDAS 365
           + G+H VE+FDP K Q      K++ID++
Sbjct: 305 DDGYHAVEVFDPLKAQATYMICKEYIDST 330

BLAST of Spg031391 vs. ExPASy TrEMBL
Match: A0A5N6QDB2 (Abhydrolase_3 domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_001830 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 2.2e-109
Identity = 192/323 (59.44%), Postives = 243/323 (75.23%), Query Frame = 0

Query: 39  ATATTSIIDPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTF 98
           A  T+SI DP++ L I  NPDGS+TR   FPT   T T   +   A+SKD+PL P+ NTF
Sbjct: 2   ADQTSSIGDPYKFLKISPNPDGSLTRLAPFPT-ASTNTESNSAQLALSKDIPLNPTTNTF 61

Query: 99  IRLYKPHNIPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYRL 158
           +RL+ PH  P N   KLP+++Y HGGGF+ FS  S+PFH  CSRM+A++PA++ S EYRL
Sbjct: 62  LRLFLPHPPPPN--QKLPLIIYFHGGGFVLFSATSQPFHDSCSRMAAYIPAVVASVEYRL 121

Query: 159 APEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGGNITFNA 218
           APEHRLPAAYDDAV+A+ W+R QA  +   +C+ WL+E  DF+RCFL GSS+GGNI ++A
Sbjct: 122 APEHRLPAAYDDAVDAVMWVRNQAKDV---NCDDWLREYVDFTRCFLMGSSAGGNIAYHA 181

Query: 219 AVRALDCDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPEDCD 278
            +R LD DL PV+I G + NQP+FGG+ERT SE+R  NDRILPL  NDLMW LALP+D D
Sbjct: 182 GLRVLDVDLEPVEIQGLILNQPYFGGVERTGSEMRFINDRILPLAANDLMWALALPKDAD 241

Query: 279 RSHWFCDPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFHEGG 338
           R H +C+  V G H  KIGRL RCLVRG+GGDPL+D+QKE V++LEAR VHV ++F + G
Sbjct: 242 RDHEYCNSMVGGSHGGKIGRLPRCLVRGYGGDPLVDKQKELVRVLEARQVHVVAQFADEG 301

Query: 339 FHGVELFDPSKLQTLVDDVKDFI 362
           FHGVELFDP+K Q+L+ DVK FI
Sbjct: 302 FHGVELFDPAKAQSLLIDVKSFI 318

BLAST of Spg031391 vs. TAIR 10
Match: AT2G45600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.2e-99
Identity = 172/318 (54.09%), Postives = 226/318 (71.07%), Query Frame = 0

Query: 47  DPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYKPHN 106
           DP++ LNI LN DGS+TR   FP LPPT+          SKD+PL  +NNTFIR++KP N
Sbjct: 9   DPYKFLNITLNSDGSLTRHRDFPKLPPTEQ---------SKDIPLNQTNNTFIRIFKPRN 68

Query: 107 IPQNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYRLAPEHRLPA 166
           IP    +KLPI+VY HGGGF+ +S AS PFH  C++M+  L  +I+S EYRLAPEHRLPA
Sbjct: 69  IPPE--SKLPILVYFHGGGFILYSAASAPFHESCTKMADRLQTIILSVEYRLAPEHRLPA 128

Query: 167 AYDDAVEAIGWLRRQADG-IDSGDCEPWLKECADFSRCFLFGSSSGGNITFNAAVRALDC 226
           AY+DAVEAI WLR QA G I+ GDC+ WLK+  DFS+C++ GSSSGGNI +N A+R +D 
Sbjct: 129 AYEDAVEAILWLRDQARGPINGGDCDTWLKDGVDFSKCYVMGSSSGGNIVYNVALRVVDT 188

Query: 227 DLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPEDCDRSHWFCD 286
           DLSPVKI G + NQ FFGG+E ++SE R  +D+I PLP   L+W L LP+  DR H + +
Sbjct: 189 DLSPVKIQGLIMNQAFFGGVEPSDSESRLKDDKICPLPATHLLWSLCLPDGVDRDHVYSN 248

Query: 287 P-TVEGPHQ-EKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFHEGGFHGVE 346
           P    GP + +K+GR    L+ G+GGDPL+DRQ+   ++L+ RGVHVE+RF + GFH  E
Sbjct: 249 PIKSSGPQEKDKMGRFPSTLINGYGGDPLVDRQRHVAEMLKGRGVHVETRFDKDGFHACE 308

Query: 347 LFDPSKLQTLVDDVKDFI 362
           LFD +K + L + V+ F+
Sbjct: 309 LFDGNKAKALYETVEAFM 315

BLAST of Spg031391 vs. TAIR 10
Match: AT2G45610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 5.2e-87
Identity = 150/317 (47.32%), Postives = 216/317 (68.14%), Query Frame = 0

Query: 47  DPFEHLNIKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYKPHN 106
           DP++HLNI +NP+GS TR   +P + P   P P    A SKD+ +       +R+++P N
Sbjct: 10  DPYKHLNITINPNGSCTRHFVWPRVEPDPDPCPG-KLAASKDVTINHETGVSVRIFRPTN 69

Query: 107 IP--QNAINKLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYRLAPEHRL 166
           +P   NA+ +LPI+++ HG G++ +   S      CS+M++ L  ++VS  YRL PEHRL
Sbjct: 70  LPSNDNAVARLPIIIHLHGSGWILYPANSAANDRCCSQMASELTVIVVSVHYRLPPEHRL 129

Query: 167 PAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGGNITFNAAVRALD 226
           PA YDDA++A+ W+++Q   +DS + EPWLK+ ADFSRC++ GSS+G NI F  A+R+LD
Sbjct: 130 PAQYDDALDALLWVKQQV--VDSTNGEPWLKDYADFSRCYICGSSNGANIAFQLALRSLD 189

Query: 227 CDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPEDCDRSHWFC 286
            DL+P++I G VF QP FGG  RT+SEL++  D ++P+P  D MW L+LP   DR H +C
Sbjct: 190 HDLTPLQIDGCVFYQPLFGGKTRTKSELKNFADPVMPVPAVDAMWELSLPVGVDRDHRYC 249

Query: 287 DPTVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFVKLLEARGVHVESRFHEGGFHGVEL 346
           +P    P +EK+GRL RCLV G+GGD  +DRQ++FV LL A GV VE+RF + GFH +EL
Sbjct: 250 NPLGYLPQKEKVGRLGRCLVIGYGGDTSLDRQQDFVNLLVAAGVRVEARFDDAGFHSIEL 309

Query: 347 FDPSKLQTLVDDVKDFI 362
            DP +   L++ ++DFI
Sbjct: 310 VDPRRAVALLNMIRDFI 323

BLAST of Spg031391 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 293.5 bits (750), Expect = 2.3e-79
Identity = 157/330 (47.58%), Postives = 215/330 (65.15%), Query Frame = 0

Query: 45  IIDPFEHLNIKLNPDGSITRP-NQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYK 104
           I DP+ +LNI  NPDGSITR  + FP    T  P P ++PA+SKDLP+    +T++RLY 
Sbjct: 7   IADPYAYLNIVNNPDGSITRDLSNFPCTAATPDPSP-LNPAVSKDLPVNQLKSTWLRLY- 66

Query: 105 PHNIPQNAIN-------KLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEY 164
              +P +A+N       KLPIVVY HGGGF+  SV  + FH +CS ++  L A++VS  Y
Sbjct: 67  ---LPSSAVNEGNVSSQKLPIVVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSY 126

Query: 165 RLAPEHRLPAAYDDAVEAIGWLRRQADGIDSGDCEPWLKECADFSRCFLFGSSSGGNITF 224
           RLAPEHRLPAAYDD VEA+ W++   D         W+K  ADFS  FL G+S+GGN+ +
Sbjct: 127 RLAPEHRLPAAYDDGVEALDWIKTSDD--------EWIKSHADFSNVFLMGTSAGGNLAY 186

Query: 225 NAAVRALD--CDLSPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALP 284
           N  +R++D   DLSP++I G + + PFFGG ER+ESE+R  ND++ P  V D+MW L+LP
Sbjct: 187 NVGLRSVDSVSDLSPLQIRGLILHHPFFGGEERSESEIRLMNDQVCPPIVTDVMWDLSLP 246

Query: 285 EDCDRSHWFCDPTV-EGPHQ-EKIGRLR-RCLVRGHGGDPLIDRQKEFVKLLEARGVHVE 344
              DR H + +PTV +G  + EKIGRLR + ++ G   DP+ID QK+  KL++ +GV V 
Sbjct: 247 VGVDRDHEYSNPTVGDGSEKLEKIGRLRWKVMMIGGEDDPMIDLQKDVAKLMKKKGVEVV 306

Query: 345 SRFHEGGFHGVELFDPSKLQTLVDDVKDFI 362
             +  G  HG E+ DPSK +TL   +K+FI
Sbjct: 307 EHYTGGHVHGAEIRDPSKRKTLFLSIKNFI 323

BLAST of Spg031391 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.7e-42
Identity = 115/296 (38.85%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 54  IKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYKPHNIPQNAIN 113
           ++L  +G++ R      L   Q P  N    + KD      NN  +RLYKP  I  +   
Sbjct: 18  LQLLSNGTVLRSESI-DLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKP--ISASNRT 77

Query: 114 KLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYRLAPEHRLPAAYDDAVE 173
            LP+VV+ HGGGF F S +   FH++C  +++ L AL+VS +YRLAPEHRLPAA++DA  
Sbjct: 78  ALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFEDAEA 137

Query: 174 AIGWLRRQA--DGIDSGDCEPWLKE--CADFSRCFLFGSSSGGNITFNAAVR--ALDCDL 233
            + WL  QA  DG++      W ++    DF R F+ G SSGGNI    AVR  +   +L
Sbjct: 138 VLTWLWDQAVSDGVNH-----WFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSIEL 197

Query: 234 SPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPEDCDRSHWFCDP- 293
           +PV++ G+V   PFFGG ERT SE    ++ +L L + D  W L+LP    R H   +P 
Sbjct: 198 TPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMANPF 257

Query: 294 TVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFV-KLLEARGVHVESRFHEGGFHG 342
               P  E I  L   LV   G + L DR KE+  KL +  G  V+    E   HG
Sbjct: 258 GPTSPTLESIS-LEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHG 303

BLAST of Spg031391 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.7e-42
Identity = 115/296 (38.85%), Postives = 157/296 (53.04%), Query Frame = 0

Query: 54  IKLNPDGSITRPNQFPTLPPTQTPIPNIDPAISKDLPLKPSNNTFIRLYKPHNIPQNAIN 113
           ++L  +G++ R      L   Q P  N    + KD      NN  +RLYKP  I  +   
Sbjct: 18  LQLLSNGTVLRSESI-DLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKP--ISASNRT 77

Query: 114 KLPIVVYGHGGGFLFFSVASEPFHHWCSRMSAHLPALIVSFEYRLAPEHRLPAAYDDAVE 173
            LP+VV+ HGGGF F S +   FH++C  +++ L AL+VS +YRLAPEHRLPAA++DA  
Sbjct: 78  ALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAFEDAEA 137

Query: 174 AIGWLRRQA--DGIDSGDCEPWLKE--CADFSRCFLFGSSSGGNITFNAAVR--ALDCDL 233
            + WL  QA  DG++      W ++    DF R F+ G SSGGNI    AVR  +   +L
Sbjct: 138 VLTWLWDQAVSDGVNH-----WFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGSGSIEL 197

Query: 234 SPVKIVGHVFNQPFFGGIERTESELRSANDRILPLPVNDLMWGLALPEDCDRSHWFCDP- 293
           +PV++ G+V   PFFGG ERT SE    ++ +L L + D  W L+LP    R H   +P 
Sbjct: 198 TPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHHMANPF 257

Query: 294 TVEGPHQEKIGRLRRCLVRGHGGDPLIDRQKEFV-KLLEARGVHVESRFHEGGFHG 342
               P  E I  L   LV   G + L DR KE+  KL +  G  V+    E   HG
Sbjct: 258 GPTSPTLESIS-LEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHG 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023526606.11.4e-15880.00probable carboxylesterase 8 [Cucurbita pepo subsp. pepo][more]
XP_022924288.12.0e-15779.40probable carboxylesterase 8 [Cucurbita moschata][more]
KAG6582576.15.9e-14174.10putative carboxylesterase 8, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_030930396.13.6e-11461.66probable carboxylesterase 8 [Quercus lobata][more]
XP_023928443.18.0e-11461.89probable carboxylesterase 8 [Quercus suber][more]
Match NameE-valueIdentityDescription
O646401.7e-9854.09Probable carboxylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=CXE8 PE=2 SV=1[more]
O646417.3e-8647.32Probable carboxylesterase 9 OS=Arabidopsis thaliana OX=3702 GN=CXE9 PE=2 SV=1[more]
Q9LVB83.3e-7847.58Probable carboxylesterase 120 OS=Arabidopsis thaliana OX=3702 GN=CXE20 PE=2 SV=1[more]
Q0ZPV73.4e-7546.67Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
I3PLR29.9e-7543.613-O-acetylpapaveroxine carboxylesterase CXE1 OS=Papaver somniferum OX=3469 GN=CX... [more]
Match NameE-valueIdentityDescription
A0A6J1EC019.7e-15879.40probable carboxylesterase 8 OS=Cucurbita moschata OX=3662 GN=LOC111431817 PE=4 S... [more]
A0A7N2MDV01.7e-11461.66Abhydrolase_3 domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1[more]
A0A6P4AD251.5e-11059.29probable carboxylesterase 8 OS=Ziziphus jujuba OX=326968 GN=LOC107427168 PE=4 SV... [more]
A0A1Q3C9633.4e-11059.27Abhydrolase_3 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CF... [more]
A0A5N6QDB22.2e-10959.44Abhydrolase_3 domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_... [more]
Match NameE-valueIdentityDescription
AT2G45600.11.2e-9954.09alpha/beta-Hydrolases superfamily protein [more]
AT2G45610.15.2e-8747.32alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.12.3e-7947.58carboxyesterase 20 [more]
AT5G06570.11.7e-4238.85alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.21.7e-4238.85alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (cylindrica) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 118..342
e-value: 1.3E-46
score: 159.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 49..364
e-value: 5.1E-75
score: 255.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 72..362
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..39
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 42..361
NoneNo IPR availablePANTHERPTHR23024:SF491ALPHA/BETA HYDROLASE FOLD PROTEINcoord: 42..361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spg031391.1Spg031391.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity